FvH4_2g41261

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017145
Physical Location & Seq
Reverse (-)
29236440 .. 29236742
303 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g41261.t1

Sequence Viewer

Length: 303 bp
ATGGAGCTAACAGCGGAGGAGCTGATACAATACAACGGCACGGACCCATCGAAGCCCATATACGTGGCAGTCAAGGGCCGCGTCTTCGACGTGAGCAACGGCAAGTCGTTTTACGGTCCTGGAGGTCCCTACGCCATGTTTGCCGGCAAGGACGCCAGCCGAGCTCTGGCCAAGATGACCAAGAACGAAGAAGACATCACCGCCGACCTCCAGGGCCTCTCCGACAAGGAGATCGGCGTCCTCACCGACTGGGAGAACAAGTTCGTAGCTAAGTACCCTGTTGTTGGCCGTGTTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0012505 GO:0016020 GO:0020037 GO:0044424 GO:0044444 GO:0044464 GO:0046906 GO:0048037 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

101

Amino Acids

10.89

Weight (kDa)

5.07

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 4 - 97 1.8e-13 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24940
fragaria_vesca FvH4_2g41261
malus_domestica MD15G1042800.v1.1
prunus_persica Prupe.1G396100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0299251
rosa_laevigata RLG00000011449
rosa_multiflora Rmu_sc0001434.1_g000006
rosa_roxburghii Rroxscaffold_7G00168870
rosa_rugosa Rorug06G0286600
rosa_samantha Rh6AG397900 Rh6CG412500 Rh6DG399200
rosa_wichuraiana Rw6G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 81
AciI CCGC 3 cut(s) 14, 79, 201
AcoI YGGCCR 2 cut(s) 168, 286
AcyI GRCGYC 2 cut(s) 153, 237
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 2 cut(s) 166, 284
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 2 cut(s) 118, 210
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 4 cut(s) 7, 22, 164, 269
AluI AGCT 4 cut(s) 7, 22, 164, 269
Alw21I GWGCWC 1 cut(s) 166
AoxI GGCC 4 cut(s) 76, 168, 214, 286
Asp700I GAANNNNTTC 1 cut(s) 260
AspS9I GGNCC 5 cut(s) 43, 76, 116, 125, 214
AsuHPI GGTGA 2 cut(s) 190, 235
AvaII GGWCC 3 cut(s) 43, 116, 125
BalI TGGCCA 1 cut(s) 170
BanII GRGCYC 1 cut(s) 166
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BbsI GAAGAC 2 cut(s) 76, 198
Bbv12I GWGCWC 1 cut(s) 166
BccI CCATC 1 cut(s) 55
BceAI ACGGC 3 cut(s) 52, 115, 273
BciT130I CCWGG 2 cut(s) 120, 212
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 2 cut(s) 120, 212
Bme18I GGWCC 3 cut(s) 43, 116, 125
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 5 cut(s) 43, 76, 116, 125, 214
BmiI GGNNCC 2 cut(s) 45, 127
BmrFI CCNGG 2 cut(s) 120, 212
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpiI GAAGAC 2 cut(s) 76, 198
BpmI CTGGAG 2 cut(s) 141, 194
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 2 cut(s) 153, 237
BsaJI CCNNGG 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 284
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 254
BseBI CCWGG 2 cut(s) 120, 212
BseDI CCNNGG 1 cut(s) 211
BseLI CCNNNNNNNGG 2 cut(s) 166, 284
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 78, 170, 216, 288
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 166, 284
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 4 cut(s) 78, 170, 216, 288
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 3 cut(s) 14, 79, 201
BspANI GGCC 4 cut(s) 78, 170, 216, 288
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 45, 127
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 2 cut(s) 153, 237
Bst2UI CCWGG 2 cut(s) 120, 212
Bst4CI ACNGT 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 153, 237
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 2 cut(s) 145, 157
BstDEI CTNAG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 1 cut(s) 234
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 140, 161
BstNI CCWGG 2 cut(s) 120, 212
BstSCI CCNGG 2 cut(s) 118, 210
BstUI CGCG 1 cut(s) 81
BstV2I GAAGAC 2 cut(s) 76, 198
BstXI CCANNNNNNTGG 1 cut(s) 64
BsuRI GGCC 4 cut(s) 78, 170, 216, 288
BtrI CACGTC 1 cut(s) 91
Cac8I GCNNGC 2 cut(s) 145, 157
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 5 cut(s) 43, 76, 116, 125, 214
CseI GACGC 3 cut(s) 70, 161, 226
Csp6I GTAC 1 cut(s) 274
CviAII CATG 1 cut(s) 136
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 270
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
EaeI YGGCCR 2 cut(s) 168, 286
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 166
Eco47I GGWCC 3 cut(s) 43, 116, 125
Eco53kI GAGCTC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 164
EcoO109I RGGNCCY 2 cut(s) 125, 214
EcoRII CCWGG 2 cut(s) 118, 210
EcoT38I GRGCYC 1 cut(s) 166
FaeI CATG 1 cut(s) 139
FaiI YATR 3 cut(s) 59, 61, 137
FaqI GGGAC 1 cut(s) 111
FatI CATG 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 79
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 2 cut(s) 141, 194
HaeIII GGCC 4 cut(s) 78, 170, 216, 288
HapII CCGG 1 cut(s) 144
HgaI GACGC 3 cut(s) 70, 161, 226
Hin1I GRCGYC 2 cut(s) 153, 237
Hin1II CATG 1 cut(s) 139
HpaII CCGG 1 cut(s) 144
HphI GGTGA 2 cut(s) 190, 235
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 2 cut(s) 63, 90
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 161
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 2 cut(s) 63, 90
Hsp92I GRCGYC 2 cut(s) 153, 237
Hsp92II CATG 1 cut(s) 139
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 4, 19
LpnPI CCDG 9 cut(s) 105, 132, 152, 157, 169, 197, 224, 235, 291
MaeII ACGT 2 cut(s) 63, 90
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 3 cut(s) 76, 200, 203
MhlI GDGCHC 1 cut(s) 166
MlsI TGGCCA 1 cut(s) 170
MluNI TGGCCA 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 5 cut(s) 10, 116, 218, 227, 251
Mox20I TGGCCA 1 cut(s) 170
MroNI GCCGGC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 260
MscI TGGCCA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 62
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 120, 212
MvaI CCWGG 2 cut(s) 120, 212
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 140, 161
NaeI GCCGGC 1 cut(s) 145
NdeII GATC 1 cut(s) 231
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 2 cut(s) 45, 127
NmeAIII GCCGAG 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 64
PpuMI RGGWCCY 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 118, 210
PspGI CCWGG 2 cut(s) 118, 210
PspN4I GGNNCC 2 cut(s) 45, 127
PspPI GGNCC 5 cut(s) 43, 76, 116, 125, 214
PspPPI RGGWCCY 1 cut(s) 125
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 166
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 5 cut(s) 43, 76, 116, 125, 214
ScrFI CCNGG 2 cut(s) 120, 212
SduI GDGCHC 1 cut(s) 166
SetI ASST 8 cut(s) 9, 24, 66, 93, 127, 166, 210, 271
SinI GGWCC 3 cut(s) 43, 116, 125
SmiMI CAYNNNNRTG 1 cut(s) 62
SsiI CCGC 3 cut(s) 14, 79, 201
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 118, 210
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 2 cut(s) 66, 93
TaqI TCGA 2 cut(s) 50, 87
TauI GCSGC 1 cut(s) 81
TspGWI ACGGA 1 cut(s) 56
VpaK11BI GGWCC 3 cut(s) 43, 116, 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.