Rw6G034830

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017145
Physical Location & Seq
Forward (+)
59455169 .. 59456360
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034830.1

Sequence Viewer

Length: 309 bp
ATGGCGATGGAGCTAAAAGCAGAGGAGCTGAGACAGTACAACGGCACTGACCCATCGAAGCCGATATACGTGGCGGTGAAGAGCCGCATCTTCGACGTGACCAACGGCAAGTCGTTTTACGGTCCTGGAGGTCCCTACGCCATGTTCGCAGGCAAGGACGCCAGCCGAGCTCTGGCCAAGATGACCAAGAACGAAGAAGACATCACCGCCGACCTCCAAGGCCTCTCCGACAAGGAGATCGGCGTCCTCACCGACTGGGAGAACAAGTTCGAGGCTAAGTACCCCGTTGTTGGCCGTGTTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0012505 GO:0016020 GO:0020037 GO:0044424 GO:0044444 GO:0044464 GO:0046906 GO:0048037 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

102

Amino Acids

11.25

Weight (kDa)

5.44

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 7 - 73 1.7e-12 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24940
fragaria_vesca FvH4_2g41261
malus_domestica MD15G1042800.v1.1
prunus_persica Prupe.1G396100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0299251
rosa_laevigata RLG00000011449
rosa_multiflora Rmu_sc0001434.1_g000006
rosa_roxburghii Rroxscaffold_7G00168870
rosa_rugosa Rorug06G0286600
rosa_samantha Rh6AG397900 Rh6CG412500 Rh6DG399200
rosa_wichuraiana Rw6G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 74, 85, 207
AcoI YGGCCR 2 cut(s) 174, 292
AcyI GRCGYC 2 cut(s) 159, 243
AfaI GTAC 2 cut(s) 38, 281
AfiI CCNNNNNNNGG 2 cut(s) 172, 290
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 124
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 3 cut(s) 13, 28, 170
AluI AGCT 3 cut(s) 13, 28, 170
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 25
AoxI GGCC 3 cut(s) 174, 220, 292
Asp700I GAANNNNTTC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 122, 131
AsuHPI GGTGA 3 cut(s) 88, 196, 241
AvaII GGWCC 2 cut(s) 122, 131
BalI TGGCCA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 172
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 172
BccI CCATC 1 cut(s) 61
BceAI ACGGC 3 cut(s) 58, 121, 279
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 25
BisI GCNGC 1 cut(s) 85
BlsI GCNGC 1 cut(s) 86
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 122, 131
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 122, 131
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 126
BmrI ACTGGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 265
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 2 cut(s) 159, 243
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 290
Bse1I ACTGG 1 cut(s) 260
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 172, 290
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 1 cut(s) 38
BshFI GGCC 3 cut(s) 176, 222, 294
BsiHKAI GWGCWC 1 cut(s) 172
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 172, 290
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 3 cut(s) 176, 222, 294
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 3 cut(s) 74, 85, 207
BspANI GGCC 3 cut(s) 176, 222, 294
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 74
BsrI ACTGG 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 237
BssNI GRCGYC 2 cut(s) 159, 243
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 36, 122
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 2 cut(s) 159, 243
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 151, 163
BstDEI CTNAG 2 cut(s) 29, 276
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 2 cut(s) 146, 167
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 204
BsuRI GGCC 3 cut(s) 176, 222, 294
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 2 cut(s) 151, 163
Cfr13I GGNCC 2 cut(s) 122, 131
CseI GACGC 2 cut(s) 167, 232
Csp6I GTAC 2 cut(s) 37, 280
CviAII CATG 1 cut(s) 142
CviQI GTAC 2 cut(s) 37, 280
DdeI CTNAG 2 cut(s) 29, 276
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EaeI YGGCCR 2 cut(s) 174, 292
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 122, 131
Eco53kI GAGCTC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 1 cut(s) 145
FaiI YATR 2 cut(s) 67, 143
FaqI GGGAC 1 cut(s) 117
FatI CATG 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 3 cut(s) 176, 222, 294
HgaI GACGC 2 cut(s) 167, 232
Hin1I GRCGYC 2 cut(s) 159, 243
Hin1II CATG 1 cut(s) 145
HphI GGTGA 3 cut(s) 88, 196, 241
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 1 cut(s) 306
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 2 cut(s) 36, 122
HpyCH4IV ACGT 2 cut(s) 69, 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 167
HpyF3I CTNAG 2 cut(s) 29, 276
HpySE526I ACGT 2 cut(s) 69, 96
Hsp92I GRCGYC 2 cut(s) 159, 243
Hsp92II CATG 1 cut(s) 145
Kzo9I GATC 1 cut(s) 237
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 2 cut(s) 10, 25
LpnPI CCDG 6 cut(s) 111, 135, 138, 158, 175, 241
LweI GCATC 1 cut(s) 96
MaeII ACGT 2 cut(s) 69, 96
MaeIII GTNAC 1 cut(s) 97
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 4 cut(s) 82, 91, 206, 209
MhlI GDGCHC 1 cut(s) 172
MlsI TGGCCA 1 cut(s) 176
MluNI TGGCCA 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 6 cut(s) 16, 122, 224, 233, 257, 265
Mox20I TGGCCA 1 cut(s) 176
MroXI GAANNNNTTC 1 cut(s) 266
MscI TGGCCA 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 146, 167
NdeII GATC 1 cut(s) 237
NlaIII CATG 1 cut(s) 145
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 97
PceI AGGCCT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 2 cut(s) 122, 131
PspPPI RGGWCCY 1 cut(s) 131
RsaI GTAC 2 cut(s) 38, 281
RsaNI GTAC 2 cut(s) 37, 280
SacI GAGCTC 1 cut(s) 172
SapI GCTCTTC 1 cut(s) 74
SatI GCNGC 1 cut(s) 85
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 2 cut(s) 122, 131
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 172
SetI ASST 7 cut(s) 15, 30, 72, 99, 133, 172, 216
SfaNI GCATC 1 cut(s) 96
SinI GGWCC 2 cut(s) 122, 131
SseBI AGGCCT 1 cut(s) 222
SsiI CCGC 3 cut(s) 74, 85, 207
SstI GAGCTC 1 cut(s) 172
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 36, 122
TaiI ACGT 2 cut(s) 72, 99
TaqI TCGA 3 cut(s) 56, 93, 270
TatI WGTACW 1 cut(s) 36
TauI GCSGC 1 cut(s) 87
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 97
TspRI CASTG 1 cut(s) 52
VpaK11BI GGWCC 2 cut(s) 122, 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.