MD15G1042800.v1.1

steroid-binding protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017145
Physical Location & Seq
Reverse (-)
2984660 .. 2984962
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1042800.v1.1.491

Sequence Viewer

Length: 303 bp
ATGGAGTTTACTGCCCAGCAGCTGATCCAGTACAACGGCACCGACCCATCGAAGCCCATATACGTGGCGCTCAAAGGCCGCGTCTTCGACGTCACGGACGGCAAGTCGTTTTACGGTCCCGGAGGGCCGTACGAGATGTTCGCCGGCAAGGACGCCAGCAGAGCTCTGGCGAAGATGAGCAAGAACGACGAGGACATCACCGCCTCCCTGGACGGCCTCACGGAGAAGGAGATCGGCGTCCTCACCGACTGGGAAAAGAAGTTCGAAGCTAAGTACCCAGTTGTCGGCCGCGTCGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0012505 GO:0016020 GO:0020037 GO:0044424 GO:0044444 GO:0044464 GO:0046906 GO:0048037 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

101

Amino Acids

10.99

Weight (kDa)

5.09

Isoelectric Point (pI)

27.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 5 - 86 6.3e-13 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24940
fragaria_vesca FvH4_2g41261
malus_domestica MD15G1042800.v1.1
prunus_persica Prupe.1G396100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0299251
rosa_laevigata RLG00000011449
rosa_multiflora Rmu_sc0001434.1_g000006
rosa_roxburghii Rroxscaffold_7G00168870
rosa_rugosa Rorug06G0286600
rosa_samantha Rh6AG397900 Rh6CG412500 Rh6DG399200
rosa_wichuraiana Rw6G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 2 cut(s) 81, 291
AciI CCGC 3 cut(s) 79, 201, 289
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 286
AcyI GRCGYC 3 cut(s) 90, 153, 237
AfaI GTAC 3 cut(s) 32, 131, 275
AfiI CCNNNNNNNGG 1 cut(s) 284
AhdI GACNNNNNGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 207
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 3 cut(s) 22, 164, 269
AluI AGCT 3 cut(s) 22, 164, 269
Alw21I GWGCWC 1 cut(s) 166
AlwI GGATC 1 cut(s) 19
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 4 cut(s) 76, 125, 214, 286
ApeKI GCWGC 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 116, 125
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 190, 235
AsuII TTCGAA 1 cut(s) 264
AvaII GGWCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 166
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 1 cut(s) 31
BccI CCATC 1 cut(s) 55
BceAI ACGGC 4 cut(s) 52, 112, 115, 229
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 3 cut(s) 20, 79, 289
BlsI GCNGC 3 cut(s) 21, 80, 290
Bme1390I CCNGG 2 cut(s) 120, 209
Bme18I GGWCC 1 cut(s) 116
BmeRI GACNNNNNGTC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 116, 125
BmiI GGNNCC 2 cut(s) 40, 118
BmrFI CCNGG 2 cut(s) 120, 209
BmrI ACTGGG 2 cut(s) 259, 272
BmuI ACTGGG 2 cut(s) 259, 272
BpiI GAAGAC 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 3 cut(s) 90, 153, 237
BsaJI CCNNGG 1 cut(s) 207
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 28, 254, 278
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 3 cut(s) 28, 254, 278
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 15
Bsh1236I CGCG 2 cut(s) 81, 291
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 4 cut(s) 78, 127, 216, 288
BshNI GGYRCC 1 cut(s) 38
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 2 cut(s) 120, 144
BsiWI CGTACG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 4 cut(s) 78, 127, 216, 288
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 2 cut(s) 24, 231
BspACI CCGC 3 cut(s) 79, 201, 289
BspANI GGCC 4 cut(s) 78, 127, 216, 288
BspFNI CGCG 2 cut(s) 81, 291
BspLI GGNNCC 2 cut(s) 40, 118
BspPI GGATC 1 cut(s) 19
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 3 cut(s) 28, 254, 278
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 2 cut(s) 24, 231
BssNI GRCGYC 3 cut(s) 90, 153, 237
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 116
BstACI GRCGYC 3 cut(s) 90, 153, 237
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 145, 157
BstDEI CTNAG 1 cut(s) 270
BstFNI CGCG 2 cut(s) 81, 291
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 2 cut(s) 27, 234
BstMBI GATC 2 cut(s) 24, 231
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 118, 207
BstUI CGCG 2 cut(s) 81, 291
BstV1I GCAGC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 76
BstXI CCANNNNNNTGG 1 cut(s) 64
BstZI CGGCCG 1 cut(s) 286
BsuRI GGCC 4 cut(s) 78, 127, 216, 288
Cac8I GCNNGC 2 cut(s) 145, 157
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 116, 125
CseI GACGC 4 cut(s) 70, 161, 226, 280
Csp6I GTAC 3 cut(s) 31, 130, 274
CviJI RGCY 8 cut(s) 22, 55, 78, 127, 164, 216, 269, 288
CviKI_1 RGCY 8 cut(s) 22, 55, 78, 127, 164, 216, 269, 288
CviQI GTAC 3 cut(s) 31, 130, 274
DdeI CTNAG 1 cut(s) 270
DpnI GATC 2 cut(s) 26, 233
DpnII GATC 2 cut(s) 24, 231
DriI GACNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 286
EagI CGGCCG 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 116
Eco52I CGGCCG 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 207
EcoT38I GRGCYC 1 cut(s) 166
FaiI YATR 2 cut(s) 59, 61
FaqI GGGAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 20, 79, 289
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 3 cut(s) 20, 79, 289
GlaI GCGC 1 cut(s) 69
GluI GCNGC 3 cut(s) 20, 79, 289
GsaI CCCAGC 1 cut(s) 19
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 4 cut(s) 78, 127, 216, 288
HapII CCGG 2 cut(s) 120, 144
HgaI GACGC 4 cut(s) 70, 161, 226, 280
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 3 cut(s) 90, 153, 237
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HpaII CCGG 2 cut(s) 120, 144
HphI GGTGA 2 cut(s) 190, 235
Hpy166II GTNNAC 1 cut(s) 9
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 3 cut(s) 92, 191, 296
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 2 cut(s) 63, 90
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 2 cut(s) 63, 90
Hsp92I GRCGYC 3 cut(s) 90, 153, 237
HspAI GCGC 1 cut(s) 68
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 24, 231
Lsp1109I GCAGC 1 cut(s) 31
MaeII ACGT 2 cut(s) 63, 90
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 26, 233
MboI GATC 2 cut(s) 24, 231
MboII GAAGA 2 cut(s) 76, 184
MhlI GDGCHC 1 cut(s) 166
MnlI CCTC 5 cut(s) 116, 184, 214, 227, 251
MroNI GCCGGC 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 2 cut(s) 120, 144
MspR9I CCNGG 2 cut(s) 120, 209
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 2 cut(s) 81, 291
MwoI GCNNNNNNNGC 1 cut(s) 161
NaeI GCCGGC 1 cut(s) 145
NciI CCSGG 1 cut(s) 120
NdeII GATC 2 cut(s) 24, 231
NgoMIV GCCGGC 1 cut(s) 143
NlaIV GGNNCC 2 cut(s) 40, 118
NmuCI GTSAC 1 cut(s) 91
NspV TTCGAA 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 145
Pfl23II CGTACG 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 3 cut(s) 21, 80, 290
Ppu21I YACGTR 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 207
PspLI CGTACG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 40, 118
PspPI GGNCC 2 cut(s) 116, 125
PsrI GAACNNNNNNTAC 2 cut(s) 122, 154
PstNI CAGNNNCTG 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 3 cut(s) 32, 131, 275
RsaNI GTAC 3 cut(s) 31, 130, 274
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 166
SatI GCNGC 3 cut(s) 20, 79, 289
Sau3AI GATC 2 cut(s) 24, 231
Sau96I GGNCC 2 cut(s) 116, 125
ScrFI CCNGG 2 cut(s) 120, 209
SduI GDGCHC 1 cut(s) 166
SetI ASST 5 cut(s) 24, 66, 93, 166, 271
SfuI TTCGAA 1 cut(s) 264
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 62
SsiI CCGC 3 cut(s) 79, 201, 289
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 118, 207
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 2 cut(s) 66, 93
TaqI TCGA 3 cut(s) 50, 87, 264
TatI WGTACW 1 cut(s) 30
TauI GCSGC 2 cut(s) 81, 291
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 91
TspGWI ACGGA 2 cut(s) 110, 236
VpaK11BI GGWCC 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.