FvH4_3g00220

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008724
Physical Location & Seq
Forward (+)
123235 .. 129411
6177 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00220.t1

Sequence Viewer

Length: 1893 bp
ATGAAGTTTATGAAAATTGGGACTAAGCCGGACACCTTCTACACTGAACGAGCTACCAGGTCTGTAGCTTCAGATGTACCCAGCGACCTTCTCATACAAATCAACAACGTCAGTTATCTTCTCCATAAGCTGCAGTTTCCATTGCTTCCAAAATGTGGGCTGTTACAGCAGCTTTGCTGTGATTCTGGTGGTCCAGAAAAAGTGAACATAGAGCTTCACGACATTCCTGGAGGCGAAGAAGCTTTTGAGCTGTGTGCTAAGTTCTGTTATGGAATAAAAATAAATCTCAGTGCCTATAACATTGTACCTGCATATTGTGCTGCTAAATTCCTTGGAATGACTGAATCTGTAGAGAAGGGAAATTTTGTCCTAAAACTCGAGGCTTTCCTCAACTCATGCATTCTCGAAGGTTGGAAAGACTCCATCATCACACTACAAAGTACTGTTAAGTTGCCTGACTGGTCTGAGAATCTTGGAATCATCAGAAAGTGCATCGATTCAATTGTTGAAAAAATCCTAACACCCCCACCAAAGGTGACATGGTCCTACACATATACCAGACCTGGTTACAACAAAAAGCATCATCATCCTGTCCCCAAGGATTGGTGGACAGAGGATATATCAGACCTTGATATAGACCTGTTTCGATGCATAGTTACTGCTGTTAGATCAACATATATGCTGCCACCACAGCTCATTGGTGAGGCTTTGCATGTATACGCTTGTCGTTGGCTACCAGACACCACAAACATTAGACCTCCTCTGGATCAAAATAGCGATTCTAATCAGGATGAAACATTAATGGAAAAGAATCGACGGATTGTTGATAACATTGCTAGCATGATTCCTGGAGATCAGGGATCGGTTTCAGTTGGTTTCTTGCTAAGGCTTCTTAATATTGCAAACTATTTAGGTGGAGTGTCTCCGGTGACAAAGACAGAACTCCTGAGGAGATCCAGTTTACAACTTGAAGAGGCGACCGTGAATGACCTGCTTATTCCTTCACACTCACCCACTGACCCTGAATTTTATGATATTGACTTGGTATTGTCACTCTTGCAAAGTTTCTTGATGAGATGGAAAAGACAATCCCCTGCAGCAGCTGCAGAAAACAGCCACCACTTTTTCAGGACAATTAGAAAGGTTGGGAAGATCATTGATTCTTACCTACAAGTCGTCGCAACAGATGCCAACATGCCAGTATCAAAAATGGCAACTCTTGCAGAAGCTTTGCCAGATATTGCTAGGGAAGACCATGATGACATCTACAAGGCTATCAACATTTATCTAAAGGAGCATTCTGATCTGAGCAAGGCAGACAAGAAGCGGCTGTGCCGCATTTTAGACTGCCAGAAGTTGTCACCAGAGGTACGTGCTCATGCTGTCAAAAATGAGAGGCTACCATTGAGGATTGTTGTGCAAGTCCTCTTCTTTGAGCAAGAGAGAGGGTCCAACTCTAAGGCAGCAGTGACATCTCATAGACTACCAGACCGGCTGGTGGGGCAACTTTCCAGCTCAGGAAAACAAACATCAACTACTAGCAGATCTGAAGATCATGCAGCTAAGCTAAGAACGGGTGGAGATGATGAAAAACTCAGTATGGGGAAGGGCACGAGAAGAACCAATAATGCAGTTTCTGAAAGCACTAATGCTCTTGGAACGAGAGGTATTTATGAGCAAAGGAAAAGATCAGAAGCAAAGTCTGCAGCGGAAATCAAGGAAAGGAAGGCAGTGAAAAGGGGTGAGATACAAGAAGTGGAAGAAGTAGATTTGGGAAGAGAAATCAGGGAGCTGGGAATAATATCAGCTGGGAGCAAGTTGGACGCTCACAAGATGATAAACAAGGGAAGTAGATCATCAGACCATGGCCGCGACAAAGCCAAAGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

631

Amino Acids

70.67

Weight (kDa)

8.42

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 201 - 456 3.6e-80 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 316, 999
AccB7I CCANNNNNTGG 2 cut(s) 155, 603
AccI GTMKAC 1 cut(s) 717
AccII CGCG 1 cut(s) 1872
AciI CCGC 4 cut(s) 1327, 1336, 1709, 1870
AclWI GGATC 3 cut(s) 774, 868, 948
AcoI YGGCCR 1 cut(s) 1867
AcsI RAATTY 3 cut(s) 326, 361, 1025
AcuI CTGAAG 2 cut(s) 54, 1569
AfaI GTAC 4 cut(s) 78, 306, 442, 1371
AfiI CCNNNNNNNGG 4 cut(s) 155, 532, 603, 1498
AgsI TTSAA 3 cut(s) 501, 509, 971
AjnI CCWGG 4 cut(s) 56, 226, 562, 847
Alw21I GWGCWC 1 cut(s) 1378
Alw26I GTCTC 1 cut(s) 927
AlwI GGATC 3 cut(s) 774, 868, 948
AlwNI CAGNNNCTG 2 cut(s) 1103, 1637
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 1 cut(s) 1867
ApoI RAATTY 3 cut(s) 326, 361, 1025
AseI ATTAAT 1 cut(s) 800
AspS9I GGNCC 3 cut(s) 191, 543, 1449
AsuHPI GGTGA 6 cut(s) 547, 713, 940, 1002, 1353, 1754
AsuNHI GCTAGC 1 cut(s) 836
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 191, 543, 1449
AxyI CCTNAGG 1 cut(s) 947
BaeGI GKGCMC 1 cut(s) 1613
BauI CACGAG 1 cut(s) 1612
BbsI GAAGAC 1 cut(s) 1257
Bbv12I GWGCWC 1 cut(s) 1378
BccI CCATC 2 cut(s) 431, 1071
BciT130I CCWGG 4 cut(s) 58, 228, 564, 849
BcoDI GTCTC 1 cut(s) 927
BfaI CTAG 3 cut(s) 837, 1245, 1539
BfmI CTRYAG 6 cut(s) 63, 131, 348, 1095, 1104, 1704
BfuAI ACCTGC 2 cut(s) 316, 999
BglII AGATCT 1 cut(s) 1544
BlpI GCTNAGC 1 cut(s) 1563
BmcAI AGTACT 1 cut(s) 442
Bme1390I CCNGG 4 cut(s) 58, 228, 564, 849
Bme18I GGWCC 3 cut(s) 191, 543, 1449
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 3 cut(s) 191, 543, 1449
BmiI GGNNCC 1 cut(s) 1450
BmrFI CCNGG 4 cut(s) 58, 228, 564, 849
BmsI GCATC 4 cut(s) 501, 589, 638, 1177
BmtI GCTAGC 1 cut(s) 840
BpiI GAAGAC 1 cut(s) 1257
BpmI CTGGAG 2 cut(s) 249, 870
Bpu10I CCTNAGC 2 cut(s) 884, 1516
Bpu1102I GCTNAGC 1 cut(s) 1563
Bsa29I ATCGAT 1 cut(s) 495
BsaAI YACGTR 1 cut(s) 1373
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 3 cut(s) 331, 597, 1864
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 2 cut(s) 943, 973
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 532, 603, 1498
Bse118I RCCGGY 1 cut(s) 1491
Bse1I ACTGG 3 cut(s) 464, 957, 1199
Bse21I CCTNAGG 1 cut(s) 947
Bse3DI GCAATG 2 cut(s) 140, 831
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 4 cut(s) 58, 228, 564, 849
BseCI ATCGAT 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 331, 597, 1864
BseGI GGATG 2 cut(s) 586, 796
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 4 cut(s) 155, 532, 603, 1498
BseMI GCAATG 2 cut(s) 140, 831
BseMII CTCAG 6 cut(s) 301, 456, 938, 1298, 1530, 1609
BseNI ACTGG 3 cut(s) 464, 957, 1199
BseRI GAGGAG 2 cut(s) 750, 964
BseSI GKGCMC 1 cut(s) 1613
BseYI CCCAGC 3 cut(s) 80, 1792, 1808
Bsh1236I CGCG 1 cut(s) 1872
Bsh1285I CGRYCG 1 cut(s) 981
BshFI GGCC 1 cut(s) 1869
BshVI ATCGAT 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 981
BsiHKAI GWGCWC 1 cut(s) 1378
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 3 cut(s) 29, 926, 1492
BslFI GGGAC 2 cut(s) 34, 578
BslI CCNNNNNNNGG 4 cut(s) 155, 532, 603, 1498
BsmAI GTCTC 1 cut(s) 927
BsmFI GGGAC 2 cut(s) 34, 578
BsmI GAATGC 2 cut(s) 399, 1297
BsnI GGCC 1 cut(s) 1869
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 1378, 1613
Bsp1720I GCTNAGC 1 cut(s) 1563
Bsp19I CCATGG 1 cut(s) 1864
BspACI CCGC 4 cut(s) 1327, 1336, 1709, 1870
BspANI GGCC 1 cut(s) 1869
BspCNI CTCAG 6 cut(s) 300, 457, 939, 1299, 1529, 1608
BspDI ATCGAT 1 cut(s) 495
BspFNI CGCG 1 cut(s) 1872
BspLI GGNNCC 1 cut(s) 1450
BspMAI CTGCAG 4 cut(s) 135, 1099, 1108, 1708
BspMI ACCTGC 2 cut(s) 316, 999
BspOI GCTAGC 1 cut(s) 840
BspPI GGATC 3 cut(s) 774, 868, 948
BsrDI GCAATG 2 cut(s) 140, 831
BsrFI RCCGGY 1 cut(s) 1491
BsrI ACTGG 3 cut(s) 464, 957, 1199
BssAI RCCGGY 1 cut(s) 1491
BssECI CCNNGG 3 cut(s) 331, 597, 1864
BssNAI GTATAC 1 cut(s) 718
BssSI CACGAG 1 cut(s) 1612
BssT1I CCWWGG 3 cut(s) 331, 597, 1864
Bst1107I GTATAC 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 1612
Bst2UI CCWGG 4 cut(s) 58, 228, 564, 849
Bst4CI ACNGT 2 cut(s) 445, 982
Bst6I CTCTTC 3 cut(s) 966, 1433, 1771
BstAPI GCANNNNNTGC 5 cut(s) 317, 1103, 1187, 1220, 1703
BstBAI YACGTR 1 cut(s) 1373
BstC8I GCNNGC 1 cut(s) 838
BstDSI CCRYGG 1 cut(s) 1864
BstF5I GGATG 2 cut(s) 586, 796
BstFNI CGCG 1 cut(s) 1872
BstMAI GTCTC 1 cut(s) 927
BstMCI CGRYCG 1 cut(s) 981
BstMWI GCNNNNNNNGC 8 cut(s) 166, 317, 691, 1103, 1187, 1220, 1501, 1703
BstNI CCWGG 4 cut(s) 58, 228, 564, 849
BstNSI RCATGY 2 cut(s) 716, 1198
BstSCI CCNGG 4 cut(s) 56, 226, 562, 847
BstSFI CTRYAG 6 cut(s) 63, 131, 348, 1095, 1104, 1704
BstSLI GKGCMC 1 cut(s) 1613
BstUI CGCG 1 cut(s) 1872
BstV2I GAAGAC 1 cut(s) 1257
BstX2I RGATCY 2 cut(s) 953, 1544
BstYI RGATCY 2 cut(s) 953, 1544
BstZ17I GTATAC 1 cut(s) 718
Bsu15I ATCGAT 1 cut(s) 495
Bsu36I CCTNAGG 1 cut(s) 947
BsuRI GGCC 1 cut(s) 1869
BsuTUI ATCGAT 1 cut(s) 495
BtgI CCRYGG 1 cut(s) 1864
BtsCI GGATG 2 cut(s) 586, 796
BtsI GCAGTG 2 cut(s) 1473, 1737
BtsIMutI CAGTG 5 cut(s) 42, 295, 1014, 1473, 1737
BveI ACCTGC 2 cut(s) 316, 999
Cac8I GCNNGC 1 cut(s) 838
CaiI CAGNNNCTG 2 cut(s) 1103, 1637
Cfr10I RCCGGY 1 cut(s) 1491
Cfr13I GGNCC 3 cut(s) 191, 543, 1449
ClaI ATCGAT 1 cut(s) 495
CseI GACGC 1 cut(s) 1832
CsiI ACCWGGT 2 cut(s) 56, 562
Csp6I GTAC 4 cut(s) 77, 305, 441, 1370
CviAII CATG 9 cut(s) 396, 540, 713, 841, 1195, 1256, 1379, 1556, 1865
CviQI GTAC 4 cut(s) 77, 305, 441, 1370
EaeI YGGCCR 1 cut(s) 1867
Eam1104I CTCTTC 3 cut(s) 966, 1433, 1771
EarI CTCTTC 3 cut(s) 966, 1433, 1771
Eco130I CCWWGG 3 cut(s) 331, 597, 1864
Eco47I GGWCC 3 cut(s) 191, 543, 1449
Eco57I CTGAAG 2 cut(s) 54, 1569
Eco81I CCTNAGG 1 cut(s) 947
Eco88I CYCGRG 1 cut(s) 377
EcoRII CCWGG 4 cut(s) 56, 226, 562, 847
EcoT14I CCWWGG 3 cut(s) 331, 597, 1864
EcoT22I ATGCAT 2 cut(s) 401, 653
ErhI CCWWGG 3 cut(s) 331, 597, 1864
FaeI CATG 9 cut(s) 399, 543, 716, 844, 1198, 1259, 1382, 1559, 1868
FaqI GGGAC 2 cut(s) 34, 578
FatI CATG 9 cut(s) 395, 539, 712, 840, 1194, 1255, 1378, 1555, 1864
FblI GTMKAC 1 cut(s) 717
FokI GGATG 2 cut(s) 573, 803
FspBI CTAG 3 cut(s) 837, 1245, 1539
GsaI CCCAGC 3 cut(s) 84, 1796, 1812
GsuI CTGGAG 2 cut(s) 249, 870
HaeIII GGCC 1 cut(s) 1869
HapII CCGG 3 cut(s) 29, 926, 1492
HgaI GACGC 1 cut(s) 1832
Hin1II CATG 9 cut(s) 399, 543, 716, 844, 1198, 1259, 1382, 1559, 1868
HindIII AAGCTT 2 cut(s) 240, 1227
HpaII CCGG 3 cut(s) 29, 926, 1492
HphI GGTGA 6 cut(s) 547, 713, 940, 1002, 1353, 1754
Hpy166II GTNNAC 4 cut(s) 205, 609, 718, 962
Hpy188III TCNNGA 9 cut(s) 194, 218, 404, 764, 788, 946, 1069, 1129, 1518
Hpy8I GTNNAC 4 cut(s) 205, 609, 718, 962
Hpy99I CGWCG 2 cut(s) 819, 1181
HpyAV CCTTC 7 cut(s) 46, 98, 349, 401, 1011, 1600, 1720
HpyCH4III ACNGT 2 cut(s) 445, 982
HpyCH4IV ACGT 2 cut(s) 108, 1372
HpyF10VI GCNNNNNNNGC 8 cut(s) 166, 317, 691, 1103, 1187, 1220, 1501, 1703
HpySE526I ACGT 2 cut(s) 108, 1372
Hsp92II CATG 9 cut(s) 399, 543, 716, 844, 1198, 1259, 1382, 1559, 1868
LmnI GCTCC 3 cut(s) 1294, 1789, 1812
LweI GCATC 4 cut(s) 501, 589, 638, 1177
MabI ACCWGGT 2 cut(s) 56, 562
MaeI CTAG 3 cut(s) 837, 1245, 1539
MaeII ACGT 2 cut(s) 108, 1372
MaeIII GTNAC 8 cut(s) 162, 535, 566, 655, 928, 1050, 1359, 1468
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 953, 1544
MhlI GDGCHC 2 cut(s) 1378, 1613
MluCI AATT 6 cut(s) 15, 326, 361, 501, 1025, 1134
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 3 cut(s) 392, 1476, 1800
Mph1103I ATGCAT 2 cut(s) 401, 653
MseI TTAA 3 cut(s) 447, 800, 894
MspA1I CMGCKG 3 cut(s) 1103, 1709, 1808
MspI CCGG 3 cut(s) 29, 926, 1492
MspR9I CCNGG 4 cut(s) 58, 228, 564, 849
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 399, 1297
MvaI CCWGG 4 cut(s) 58, 228, 564, 849
MvnI CGCG 1 cut(s) 1872
MwoI GCNNNNNNNGC 8 cut(s) 166, 317, 691, 1103, 1187, 1220, 1501, 1703
NcoI CCATGG 1 cut(s) 1864
NheI GCTAGC 1 cut(s) 836
NlaIII CATG 9 cut(s) 399, 543, 716, 844, 1198, 1259, 1382, 1559, 1868
NlaIV GGNNCC 1 cut(s) 1450
NmuCI GTSAC 5 cut(s) 535, 928, 1050, 1359, 1468
NsiI ATGCAT 2 cut(s) 401, 653
NspI RCATGY 2 cut(s) 716, 1198
PaeR7I CTCGAG 1 cut(s) 377
PctI GAATGC 2 cut(s) 399, 1297
PfeI GAWTC 9 cut(s) 182, 344, 469, 477, 497, 779, 811, 844, 1160
PflFI GACNNNGTC 1 cut(s) 541
PflMI CCANNNNNTGG 2 cut(s) 155, 603
PfoI TCCNGGA 2 cut(s) 226, 847
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Ppu21I YACGTR 1 cut(s) 1373
PshBI ATTAAT 1 cut(s) 800
Psp6I CCWGG 4 cut(s) 56, 226, 562, 847
PspFI CCCAGC 3 cut(s) 80, 1792, 1808
PspGI CCWGG 4 cut(s) 56, 226, 562, 847
PspN4I GGNNCC 1 cut(s) 1450
PspPI GGNCC 3 cut(s) 191, 543, 1449
PspXI VCTCGAGB 1 cut(s) 377
PstI CTGCAG 4 cut(s) 135, 1099, 1108, 1708
PstNI CAGNNNCTG 2 cut(s) 1103, 1637
PsuI RGATCY 2 cut(s) 953, 1544
PsyI GACNNNGTC 1 cut(s) 541
PvuII CAGCTG 2 cut(s) 1103, 1808
RsaI GTAC 4 cut(s) 78, 306, 442, 1371
RsaNI GTAC 4 cut(s) 77, 305, 441, 1370
SaqAI TTAA 3 cut(s) 447, 800, 894
Sau96I GGNCC 3 cut(s) 191, 543, 1449
ScaI AGTACT 1 cut(s) 442
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 4 cut(s) 58, 228, 564, 849
SduI GDGCHC 2 cut(s) 1378, 1613
SexAI ACCWGGT 2 cut(s) 56, 562
SfaNI GCATC 4 cut(s) 501, 589, 638, 1177
SfcI CTRYAG 6 cut(s) 63, 131, 348, 1095, 1104, 1704
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 3 cut(s) 191, 543, 1449
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 15, 326, 361, 501, 1025, 1134
SsiI CCGC 4 cut(s) 1327, 1336, 1709, 1870
SspI AATATT 1 cut(s) 898
SspMI CTAG 3 cut(s) 837, 1245, 1539
StyD4I CCNGG 4 cut(s) 56, 226, 562, 847
StyI CCWWGG 3 cut(s) 331, 597, 1864
TaaI ACNGT 2 cut(s) 445, 982
TaiI ACGT 2 cut(s) 111, 1375
TaqI TCGA 5 cut(s) 378, 405, 495, 646, 814
TasI AATT 6 cut(s) 15, 326, 361, 501, 1025, 1134
TatI WGTACW 1 cut(s) 440
TauI GCSGC 3 cut(s) 1330, 1338, 1872
TfiI GAWTC 9 cut(s) 182, 344, 469, 477, 497, 779, 811, 844, 1160
Tru1I TTAA 3 cut(s) 447, 800, 894
Tru9I TTAA 3 cut(s) 447, 800, 894
TscAI CASTG 5 cut(s) 49, 295, 1021, 1473, 1737
TseFI GTSAC 5 cut(s) 535, 928, 1050, 1359, 1468
Tsp45I GTSAC 5 cut(s) 535, 928, 1050, 1359, 1468
TspDTI ATGAA 4 cut(s) 17, 26, 807, 1602
TspGWI ACGGA 1 cut(s) 832
TspRI CASTG 5 cut(s) 49, 295, 1021, 1473, 1737
Tth111I GACNNNGTC 1 cut(s) 541
Van91I CCANNNNNTGG 2 cut(s) 155, 603
VpaK11BI GGWCC 3 cut(s) 191, 543, 1449
VspI ATTAAT 1 cut(s) 800
XapI RAATTY 3 cut(s) 326, 361, 1025
XceI RCATGY 2 cut(s) 716, 1198
XcmI CCANNNNNNNNNTGG 1 cut(s) 537
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 717
XspI CTAG 3 cut(s) 837, 1245, 1539
ZrmI AGTACT 1 cut(s) 442
Zsp2I ATGCAT 2 cut(s) 401, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.