MD05G1363200.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008724
Physical Location & Seq
Forward (+)
47798049 .. 47803692
5644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1363200.v1.1.491

Sequence Viewer

Length: 1875 bp
ATGAAGTTTATGAAAATTGGAACCAAGCCGGACACCTTCTACACTGAAGATGCTACCAGGACCGTGATTTCAGATGTACCCAGCGACTTTGTCATACAAATTAACGACATAAGTTATCTTCTCCATACGCTGCAGTTTCCACTTCTTCCAAAATTTGGCCTCTTACAACGGCTTTGCTCTCACTCTGGTGATTCGGAAAAGATCAGCATAGAGCTTCACGATATTCCAGGAGGTGAAGATGCGTTCGAAATGTGTGCTAAGTTCTGTTACGGAATTATGATTAATCTCAGCGCCTATAACTTTGTACCTGCATTTTGTGCTGCTAAGTTCCTCCGAATGACTGAGTCAGTAGAGAAGGGAAATTTTGTTCCGAAACTTGAGGCTTTCTTCAATTCGTGCATTCTTGAAGGTTGGAAAGACTCTATTGCTGCACTACAAACTACAGTGAAGTTTCCTGAGTGGTCTGAGAATCTTGGGATCATCAGAAAGTGCATTGATTCAATTGTTGAGAAAATCCATACTCCTACAGCAAAGGTTTCATGGTCCTACACTTATACCAGGCCTGGCTACACCAAAAAGCAACATCATTCTGTCCCAAAGGATTGGTGGACTGAGGATATATCAGACCTTGATGTAGACCTGTTTCGATGTATAATTACTGCTGTTAGATCCACGTCTATGCTGCCACCGCAGCTCATTGGGGAAGCCTTGCACGTCTATGCTTGTCGTTGGCTGCCAGACACCACAAGAACACCTCCACAAACAGGTGAGGAATTTATGGAAAAGAATCGAAGAATTGTTGATAGCATTGTGAACATGATTCCCGAAGACAAGGGAGCAGTTTCTGTTGGTTTCTTGCTAAGGCTTATTGTCATTGCCAATTACTTGGGAGTGTCCCCAGTGACAAAGACAGAATTGTTAAGGAGGTCCGGTCTACAACTCGAAGAGGCAACGGTGGATGACCTGATTTTTCCTTCACACTCTCCCGCTAACCCAGAATTTTACGATATTGACTTGGTTTTGGCAGTGTTAGAAAGTTTCTTGGTGCTATGGAGAAGACAATCCCCTGCAGCTGCTTCTGAATTGAACAGAAGCGCTCAGGTTTTAGGAACAATGAGAAAGGTTGGGAAACTCATTGATTCGTACCTTCAAGTCGTTGCCAAGGATGTCAACATGCCAGTTTCAAAAGTGGTATCACTGGCTGAAGCTTTGCCGGATATTGCAAGGGAAGACCATGACAGCATCTACAAGGCTATTAACATTTATCTGAAGGAGCATGGGGATCTGAGCAAGGCAGACAAGAAGCGCCTGTGCCACATTTTAGACTGCCAGAAGTTGTCGCCTGAGGTACGTGCTCACGCTGTGAAAAATGAGCGGCTACCATTGAGGATTGTTGTGCAAGTCCTCTTCTTTGAACAAGAGAGAGACAGAGATTCCAACTCCAAGGCAGCAGCAGCAACTCCTGATCGTCGCAAACATAAACCACTGCCGGTTACACCCTCCCAGTCCCAGGAGCCAGGGAAACAAACAGTGCCAACTAGCAGAGAATCAGATAGTCATAGTCATGATAAGGTAAAATTTGGAGCAGCAAATGTTCATGATAAATTCAGTAGTAGTACCACTGGAAAGAGAGATTATGCGCTGCTGCAGATGCAAAACAAAAGATCAGACGGAAGGTTAACAGTCGAAAACGAAAGGAAAGGTGTCAGATCGGAAACGCAACAAGTAGATCATCAAGGACATAATACCAGAAGGGAAATCCTAAGAGAAGGAGCATCTGGATCTGCAGGAGCAGGGAGCAAGTTGGACGCTAAGAAGATGATCCAAAGGGGAAGTAGGCCGGACCATGGCCGCGACAAAGGTAAAGATAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

625

Amino Acids

70.22

Weight (kDa)

8.48

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 201 - 449 6.7e-80 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 1375
AccI GTMKAC 2 cut(s) 636, 934
AccII CGCG 1 cut(s) 1854
AciI CCGC 4 cut(s) 689, 987, 1375, 1852
AclWI GGATC 5 cut(s) 485, 663, 1290, 1789, 1816
AcoI YGGCCR 1 cut(s) 1849
AcsI RAATTY 6 cut(s) 152, 361, 773, 998, 1577, 1604
AcuI CTGAAG 3 cut(s) 66, 1224, 1289
AdeI CACNNNGTG 1 cut(s) 1363
AfaI GTAC 5 cut(s) 78, 306, 1145, 1350, 1618
AfeI AGCGCT 1 cut(s) 1096
AfiI CCNNNNNNNGG 4 cut(s) 155, 764, 1510, 1847
AgsI TTSAA 7 cut(s) 391, 407, 501, 1087, 1151, 1185, 1415
AjiI CACGTC 2 cut(s) 675, 715
AjnI CCWGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
AleI CACNNNNGTG 1 cut(s) 186
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 4 cut(s) 214, 694, 1073, 1208
AluI AGCT 4 cut(s) 214, 694, 1073, 1208
Alw21I GWGCWC 1 cut(s) 1357
Alw26I GTCTC 1 cut(s) 1419
AlwI GGATC 5 cut(s) 485, 663, 1290, 1789, 1816
AlwNI CAGNNNCTG 1 cut(s) 845
Aor51HI AGCGCT 1 cut(s) 1096
AoxI GGCC 4 cut(s) 157, 560, 1838, 1849
ApoI RAATTY 6 cut(s) 152, 361, 773, 998, 1577, 1604
AseI ATTAAT 1 cut(s) 282
AspLEI GCGC 4 cut(s) 293, 1097, 1308, 1642
AspS9I GGNCC 4 cut(s) 60, 543, 927, 1843
AsuHPI GGTGA 3 cut(s) 200, 245, 779
AsuII TTCGAA 1 cut(s) 246
AvaII GGWCC 4 cut(s) 60, 543, 927, 1843
AxyI CCTNAGG 1 cut(s) 1344
BbsI GAAGAC 3 cut(s) 834, 1063, 1236
Bbv12I GWGCWC 1 cut(s) 1357
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
BcoDI GTCTC 1 cut(s) 1419
BfaI CTAG 1 cut(s) 1539
BfmI CTRYAG 6 cut(s) 131, 441, 525, 1068, 1646, 1785
BfoI RGCGCY 3 cut(s) 294, 1098, 1309
BfuAI ACCTGC 1 cut(s) 316
Bme1390I CCNGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
Bme18I GGWCC 4 cut(s) 60, 543, 927, 1843
BmgBI CACGTC 2 cut(s) 675, 715
BmgT120I GGNCC 4 cut(s) 60, 543, 927, 1843
BmiI GGNNCC 2 cut(s) 22, 1515
BmrFI CCNGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
BmrI ACTGGG 2 cut(s) 893, 1498
BmsI GCATC 5 cut(s) 40, 229, 1251, 1641, 1784
BmuI ACTGGG 2 cut(s) 893, 1498
BpiI GAAGAC 3 cut(s) 834, 1063, 1236
Bpu10I CCTNAGC 2 cut(s) 860, 1098
Bpu14I TTCGAA 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 1352
BsaJI CCNNGG 5 cut(s) 1161, 1443, 1509, 1517, 1846
BsaWI WCCGGW 1 cut(s) 929
BsaXI ACNNNNNCTCC 2 cut(s) 916, 946
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 764, 1510, 1847
Bse118I RCCGGY 1 cut(s) 1489
Bse1I ACTGG 5 cut(s) 899, 1178, 1203, 1504, 1627
Bse21I CCTNAGG 1 cut(s) 1344
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
BseDI CCNNGG 5 cut(s) 1161, 1443, 1509, 1517, 1846
BseGI GGATG 2 cut(s) 964, 1171
BseLI CCNNNNNNNGG 4 cut(s) 155, 764, 1510, 1847
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 8 cut(s) 301, 333, 447, 456, 603, 1112, 1277, 1335
BseNI ACTGG 5 cut(s) 899, 1178, 1203, 1504, 1627
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 1854
BshFI GGCC 4 cut(s) 159, 562, 1840, 1851
BsiHKAI GWGCWC 1 cut(s) 1357
BsiSI CCGG 5 cut(s) 29, 930, 1214, 1490, 1841
BslFI GGGAC 3 cut(s) 578, 880, 1492
BslI CCNNNNNNNGG 4 cut(s) 155, 764, 1510, 1847
BsmAI GTCTC 1 cut(s) 1419
BsmFI GGGAC 3 cut(s) 578, 880, 1492
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 4 cut(s) 159, 562, 1840, 1851
Bsp119I TTCGAA 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 1357
Bsp19I CCATGG 1 cut(s) 1846
BspACI CCGC 4 cut(s) 689, 987, 1375, 1852
BspANI GGCC 4 cut(s) 159, 562, 1840, 1851
BspCNI CTCAG 8 cut(s) 300, 334, 448, 457, 604, 1111, 1278, 1336
BspFNI CGCG 1 cut(s) 1854
BspHI TCATGA 2 cut(s) 1564, 1597
BspLI GGNNCC 2 cut(s) 22, 1515
BspMAI CTGCAG 4 cut(s) 135, 1072, 1650, 1789
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 5 cut(s) 485, 663, 1290, 1789, 1816
BspT104I TTCGAA 1 cut(s) 246
BsrBI CCGCTC 1 cut(s) 1375
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 1489
BsrI ACTGG 5 cut(s) 899, 1178, 1203, 1504, 1627
BssAI RCCGGY 1 cut(s) 1489
BssECI CCNNGG 5 cut(s) 1161, 1443, 1509, 1517, 1846
BssT1I CCWWGG 3 cut(s) 1161, 1443, 1846
Bst2UI CCWGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
Bst4CI ACNGT 5 cut(s) 64, 445, 955, 1531, 1684
Bst6I CTCTTC 2 cut(s) 939, 1412
BstAPI GCANNNNNTGC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 1352
BstBI TTCGAA 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 1846
BstF5I GGATG 2 cut(s) 964, 1171
BstFNI CGCG 1 cut(s) 1854
BstH2I RGCGCY 3 cut(s) 294, 1098, 1309
BstHHI GCGC 4 cut(s) 293, 1097, 1308, 1642
BstMAI GTCTC 1 cut(s) 1419
BstMWI GCNNNNNNNGC 5 cut(s) 317, 688, 691, 1454, 1651
BstNI CCWGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
BstNSI RCATGY 1 cut(s) 1177
BstSCI CCNGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
BstSFI CTRYAG 6 cut(s) 131, 441, 525, 1068, 1646, 1785
BstUI CGCG 1 cut(s) 1854
BstV2I GAAGAC 3 cut(s) 834, 1063, 1236
BstX2I RGATCY 3 cut(s) 668, 1282, 1781
BstXI CCANNNNNNTGG 2 cut(s) 603, 886
BstYI RGATCY 3 cut(s) 668, 1282, 1781
Bsu36I CCTNAGG 1 cut(s) 1344
BsuRI GGCC 4 cut(s) 159, 562, 1840, 1851
BtgI CCRYGG 1 cut(s) 1846
BtrI CACGTC 2 cut(s) 675, 715
BtsCI GGATG 2 cut(s) 964, 1171
BtsI GCAGTG 2 cut(s) 1032, 1484
BtsIMutI CAGTG 8 cut(s) 42, 450, 906, 1032, 1196, 1484, 1536, 1620
BveI ACCTGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 2 cut(s) 1564, 1597
CfoI GCGC 4 cut(s) 293, 1097, 1308, 1642
Cfr10I RCCGGY 1 cut(s) 1489
Cfr13I GGNCC 4 cut(s) 60, 543, 927, 1843
CseI GACGC 1 cut(s) 1817
Csp6I GTAC 5 cut(s) 77, 305, 1144, 1349, 1617
CviAII CATG 8 cut(s) 540, 817, 1174, 1235, 1277, 1565, 1598, 1847
CviQI GTAC 5 cut(s) 77, 305, 1144, 1349, 1617
DraIII CACNNNGTG 1 cut(s) 1363
EaeI YGGCCR 1 cut(s) 1849
Eam1104I CTCTTC 2 cut(s) 939, 1412
EarI CTCTTC 2 cut(s) 939, 1412
Eco130I CCWWGG 3 cut(s) 1161, 1443, 1846
Eco147I AGGCCT 1 cut(s) 562
Eco47I GGWCC 4 cut(s) 60, 543, 927, 1843
Eco47III AGCGCT 1 cut(s) 1096
Eco57I CTGAAG 3 cut(s) 66, 1224, 1289
Eco81I CCTNAGG 1 cut(s) 1344
EcoRII CCWGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
EcoT14I CCWWGG 3 cut(s) 1161, 1443, 1846
ErhI CCWWGG 3 cut(s) 1161, 1443, 1846
FaeI CATG 8 cut(s) 543, 820, 1177, 1238, 1280, 1568, 1601, 1850
FaqI GGGAC 3 cut(s) 578, 880, 1492
FatI CATG 8 cut(s) 539, 816, 1173, 1234, 1276, 1564, 1597, 1846
FauI CCCGC 1 cut(s) 994
FblI GTMKAC 2 cut(s) 636, 934
FokI GGATG 2 cut(s) 971, 1178
FspBI CTAG 1 cut(s) 1539
GlaI GCGC 4 cut(s) 292, 1096, 1307, 1641
GsaI CCCAGC 1 cut(s) 84
HaeII RGCGCY 3 cut(s) 294, 1098, 1309
HaeIII GGCC 4 cut(s) 159, 562, 1840, 1851
HapII CCGG 5 cut(s) 29, 930, 1214, 1490, 1841
HgaI GACGC 1 cut(s) 1817
HhaI GCGC 4 cut(s) 293, 1097, 1308, 1642
Hin1II CATG 8 cut(s) 543, 820, 1177, 1238, 1280, 1568, 1601, 1850
Hin6I GCGC 4 cut(s) 291, 1095, 1306, 1640
HinP1I GCGC 4 cut(s) 291, 1095, 1306, 1640
HincII GTYRAC 2 cut(s) 1171, 1680
HindII GTYRAC 2 cut(s) 1171, 1680
HindIII AAGCTT 1 cut(s) 1206
HpaI GTTAAC 1 cut(s) 1680
HpaII CCGG 5 cut(s) 29, 930, 1214, 1490, 1841
HphI GGTGA 3 cut(s) 200, 245, 779
Hpy166II GTNNAC 6 cut(s) 609, 637, 814, 935, 1171, 1680
Hpy188III TCNNGA 8 cut(s) 218, 404, 455, 824, 1463, 1565, 1598, 1779
Hpy8I GTNNAC 6 cut(s) 609, 637, 814, 935, 1171, 1680
Hpy99I CGWCG 1 cut(s) 1473
HpyAV CCTTC 9 cut(s) 46, 349, 401, 984, 1157, 1264, 1668, 1746, 1763
HpyCH4III ACNGT 5 cut(s) 64, 445, 955, 1531, 1684
HpyCH4IV ACGT 3 cut(s) 674, 714, 1351
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 688, 691, 1454, 1651
HpySE526I ACGT 3 cut(s) 674, 714, 1351
Hsp92II CATG 8 cut(s) 543, 820, 1177, 1238, 1280, 1568, 1601, 1850
HspAI GCGC 4 cut(s) 291, 1095, 1306, 1640
KspAI GTTAAC 1 cut(s) 1680
LmnI GCTCC 7 cut(s) 836, 1273, 1513, 1583, 1772, 1790, 1797
LweI GCATC 5 cut(s) 40, 229, 1251, 1641, 1784
MaeI CTAG 1 cut(s) 1539
MaeII ACGT 3 cut(s) 674, 714, 1351
MaeIII GTNAC 3 cut(s) 266, 901, 1492
MbiI CCGCTC 1 cut(s) 1375
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 3 cut(s) 668, 1282, 1781
MhlI GDGCHC 1 cut(s) 1357
MlyI GAGTC 2 cut(s) 353, 413
MmeI TCCRAC 3 cut(s) 392, 1461, 1785
MseI TTAA 5 cut(s) 102, 282, 920, 1257, 1679
MslI CAYNNNNRTG 4 cut(s) 186, 677, 717, 1563
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 5 cut(s) 29, 930, 1214, 1490, 1841
MspR9I CCNGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
MvnI CGCG 1 cut(s) 1854
MwoI GCNNNNNNNGC 5 cut(s) 317, 688, 691, 1454, 1651
NcoI CCATGG 1 cut(s) 1846
NlaIII CATG 8 cut(s) 543, 820, 1177, 1238, 1280, 1568, 1601, 1850
NlaIV GGNNCC 2 cut(s) 22, 1515
NmuCI GTSAC 1 cut(s) 901
NspI RCATGY 1 cut(s) 1177
NspV TTCGAA 1 cut(s) 246
OliI CACNNNNGTG 1 cut(s) 186
PagI TCATGA 2 cut(s) 1564, 1597
PceI AGGCCT 1 cut(s) 562
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 8 cut(s) 191, 469, 497, 787, 820, 1139, 1433, 1547
PflFI GACNNNGTC 2 cut(s) 89, 343
PflMI CCANNNNNTGG 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 226
PleI GAGTC 2 cut(s) 352, 413
PpsI GAGTC 2 cut(s) 352, 413
Ppu21I YACGTR 1 cut(s) 1352
PshBI ATTAAT 1 cut(s) 282
Psp6I CCWGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
PspN4I GGNNCC 2 cut(s) 22, 1515
PspPI GGNCC 4 cut(s) 60, 543, 927, 1843
PstI CTGCAG 4 cut(s) 135, 1072, 1650, 1789
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 3 cut(s) 668, 1282, 1781
PsyI GACNNNGTC 2 cut(s) 89, 343
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 5 cut(s) 78, 306, 1145, 1350, 1618
RsaNI GTAC 5 cut(s) 77, 305, 1144, 1349, 1617
RseI CAYNNNNRTG 4 cut(s) 186, 677, 717, 1563
SaqAI TTAA 5 cut(s) 102, 282, 920, 1257, 1679
Sau96I GGNCC 4 cut(s) 60, 543, 927, 1843
SchI GAGTC 2 cut(s) 353, 413
ScrFI CCNGG 6 cut(s) 58, 228, 559, 564, 1511, 1518
SduI GDGCHC 1 cut(s) 1357
SfaNI GCATC 5 cut(s) 40, 229, 1251, 1641, 1784
SfcI CTRYAG 6 cut(s) 131, 441, 525, 1068, 1646, 1785
SfuI TTCGAA 1 cut(s) 246
SinI GGWCC 4 cut(s) 60, 543, 927, 1843
SmiMI CAYNNNNRTG 4 cut(s) 186, 677, 717, 1563
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 4 cut(s) 689, 987, 1375, 1852
SspMI CTAG 1 cut(s) 1539
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 6 cut(s) 56, 226, 557, 562, 1509, 1516
StyI CCWWGG 3 cut(s) 1161, 1443, 1846
TaaI ACNGT 5 cut(s) 64, 445, 955, 1531, 1684
TaiI ACGT 3 cut(s) 677, 717, 1354
TaqI TCGA 5 cut(s) 246, 646, 790, 942, 1686
TauI GCSGC 2 cut(s) 1378, 1854
TfiI GAWTC 8 cut(s) 191, 469, 497, 787, 820, 1139, 1433, 1547
Tru1I TTAA 5 cut(s) 102, 282, 920, 1257, 1679
Tru9I TTAA 5 cut(s) 102, 282, 920, 1257, 1679
TscAI CASTG 8 cut(s) 49, 450, 906, 1032, 1203, 1491, 1536, 1627
TseFI GTSAC 1 cut(s) 901
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 4 cut(s) 17, 26, 528, 1586
TspGWI ACGGA 2 cut(s) 285, 1686
TspRI CASTG 8 cut(s) 49, 450, 906, 1032, 1203, 1491, 1536, 1627
Tth111I GACNNNGTC 2 cut(s) 89, 343
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 4 cut(s) 60, 543, 927, 1843
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 6 cut(s) 152, 361, 773, 998, 1577, 1604
XceI RCATGY 1 cut(s) 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 603
XmiI GTMKAC 2 cut(s) 636, 934
XspI CTAG 1 cut(s) 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.