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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g04910 FvH4_3g04910 FvH4_3g04910 FvH4_3g04910 |
| malus_domestica | MD05G1314800.v1.1 MD10G1296200.v1.1 |
| prunus_persica | Prupe.4G044400_v2.0.a1 Prupe.4G044400_v2.0.a1 |
| pyrus_communis | pycom05g29260 pycom10g24870 |
| rosa_chinensis | RchiOBHm_Chr5g0008071 |
| rosa_laevigata | RLG00000031533 |
| rosa_multiflora | Rmu_sc0001626.1_g000002 Rmu_sc0017465.1_g000003 |
| rosa_roxburghii | Rroxscaffold_1G00068410 |
| rosa_rugosa | Rorug04G0433200 |
| rosa_samantha | Rh5AG061200 Rh5BG058000 Rh5CG068100 Rh5DG057200 |
| rosa_wichuraiana | Rw5G005940 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1775 |
| AccB1I | GGYRCC | 2 cut(s) | 1653, 2169 |
| AccBSI | CCGCTC | 1 cut(s) | 1504 |
| AccI | GTMKAC | 1 cut(s) | 2029 |
| AccII | CGCG | 2 cut(s) | 296, 2026 |
| AciI | CCGC | 6 cut(s) | 73, 171, 605, 674, 728, 1504 |
| AclWI | GGATC | 1 cut(s) | 216 |
| AcoI | YGGCCR | 1 cut(s) | 1578 |
| AcsI | RAATTY | 3 cut(s) | 919, 1342, 1475 |
| AcvI | CACGTG | 1 cut(s) | 1752 |
| AcyI | GRCGYC | 1 cut(s) | 608 |
| AfaI | GTAC | 4 cut(s) | 740, 1224, 1523, 1796 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 197, 666, 1678, 2042 |
| AflIII | ACRYGT | 2 cut(s) | 1749, 1751 |
| AgsI | TTSAA | 4 cut(s) | 514, 961, 1116, 2112 |
| AhlI | ACTAGT | 1 cut(s) | 1715 |
| AjnI | CCWGG | 2 cut(s) | 542, 1894 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 32, 64, 84, 116 |
| AluBI | AGCT | 8 cut(s) | 140, 165, 214, 468, 988, 1214, 1904, 2158 |
| AluI | AGCT | 8 cut(s) | 140, 165, 214, 468, 988, 1214, 1904, 2158 |
| Alw21I | GWGCWC | 2 cut(s) | 142, 1873 |
| Alw26I | GTCTC | 1 cut(s) | 1289 |
| Alw44I | GTGCAC | 1 cut(s) | 1869 |
| AlwI | GGATC | 1 cut(s) | 216 |
| AoxI | GGCC | 7 cut(s) | 228, 277, 1123, 1304, 1578, 1585, 2033 |
| ApaLI | GTGCAC | 1 cut(s) | 1869 |
| ApeKI | GCWGC | 4 cut(s) | 1901, 1931, 2065, 2068 |
| ApoI | RAATTY | 3 cut(s) | 919, 1342, 1475 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2011, 2043 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1770 |
| AspS9I | GGNCC | 5 cut(s) | 657, 1123, 1304, 1585, 1892 |
| AsuHPI | GGTGA | 5 cut(s) | 45, 410, 845, 1036, 2068 |
| AvaII | GGWCC | 2 cut(s) | 657, 1892 |
| BaeGI | GKGCMC | 1 cut(s) | 1873 |
| BanI | GGYRCC | 2 cut(s) | 1653, 2169 |
| BanII | GRGCYC | 2 cut(s) | 142, 1837 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1642, 1674 |
| BbrPI | CACGTG | 1 cut(s) | 1752 |
| BbsI | GAAGAC | 2 cut(s) | 438, 1257 |
| Bbv12I | GWGCWC | 2 cut(s) | 142, 1873 |
| BbvI | GCAGC | 4 cut(s) | 1913, 1918, 2052, 2080 |
| BccI | CCATC | 8 cut(s) | 479, 608, 874, 1114, 1244, 1559, 1811, 1992 |
| BceAI | ACGGC | 3 cut(s) | 184, 1565, 2048 |
| BciT130I | CCWGG | 2 cut(s) | 544, 1896 |
| BclI | TGATCA | 3 cut(s) | 772, 835, 1357 |
| BcoDI | GTCTC | 1 cut(s) | 1289 |
| BcuI | ACTAGT | 1 cut(s) | 1715 |
| BfaI | CTAG | 6 cut(s) | 275, 303, 654, 783, 1716, 2037 |
| BfmI | CTRYAG | 4 cut(s) | 234, 1085, 2012, 2066 |
| BfuAI | ACCTGC | 1 cut(s) | 1775 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1593 |
| BisI | GCNGC | 6 cut(s) | 172, 606, 1902, 1932, 2066, 2069 |
| BlpI | GCTNAGC | 1 cut(s) | 2159 |
| BlsI | GCNGC | 6 cut(s) | 173, 607, 1903, 1933, 2067, 2070 |
| Bme1390I | CCNGG | 2 cut(s) | 544, 1896 |
| Bme18I | GGWCC | 2 cut(s) | 657, 1892 |
| BmgT120I | GGNCC | 5 cut(s) | 657, 1123, 1304, 1585, 1892 |
| BmiI | GGNNCC | 3 cut(s) | 333, 1655, 2171 |
| BmrFI | CCNGG | 2 cut(s) | 544, 1896 |
| BmsI | GCATC | 5 cut(s) | 1742, 1900, 1939, 1975, 2083 |
| BpiI | GAAGAC | 2 cut(s) | 438, 1257 |
| BpmI | CTGGAG | 1 cut(s) | 1917 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2159 |
| BpuEI | CTTGAG | 2 cut(s) | 166, 1223 |
| Bsa29I | ATCGAT | 1 cut(s) | 243 |
| BsaAI | YACGTR | 1 cut(s) | 1752 |
| BsaHI | GRCGYC | 1 cut(s) | 608 |
| BsaJI | CCNNGG | 4 cut(s) | 358, 543, 1470, 1581 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 197, 666, 1678, 2042 |
| Bse118I | RCCGGY | 1 cut(s) | 197 |
| Bse1I | ACTGG | 3 cut(s) | 660, 973, 1806 |
| Bse3DI | GCAATG | 2 cut(s) | 1105, 2047 |
| BseBI | CCWGG | 2 cut(s) | 544, 1896 |
| BseCI | ATCGAT | 1 cut(s) | 243 |
| BseDI | CCNNGG | 4 cut(s) | 358, 543, 1470, 1581 |
| BseGI | GGATG | 2 cut(s) | 1360, 1423 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 197, 666, 1678, 2042 |
| BseMI | GCAATG | 2 cut(s) | 1105, 2047 |
| BseMII | CTCAG | 4 cut(s) | 199, 947, 1322, 2145 |
| BseNI | ACTGG | 3 cut(s) | 660, 973, 1806 |
| BseRI | GAGGAG | 6 cut(s) | 118, 433, 478, 634, 647, 1034 |
| BseSI | GKGCMC | 1 cut(s) | 1873 |
| BseXI | GCAGC | 4 cut(s) | 1913, 1918, 2052, 2080 |
| BseYI | CCCAGC | 1 cut(s) | 174 |
| Bsh1236I | CGCG | 2 cut(s) | 296, 2026 |
| BshFI | GGCC | 7 cut(s) | 230, 279, 1125, 1306, 1580, 1587, 2035 |
| BshNI | GGYRCC | 2 cut(s) | 1653, 2169 |
| BshVI | ATCGAT | 1 cut(s) | 243 |
| BsiHKAI | GWGCWC | 2 cut(s) | 142, 1873 |
| BsiSI | CCGG | 2 cut(s) | 198, 335 |
| BslFI | GGGAC | 2 cut(s) | 191, 424 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 197, 666, 1678, 2042 |
| BsmAI | GTCTC | 1 cut(s) | 1289 |
| BsmFI | GGGAC | 2 cut(s) | 191, 424 |
| BsmI | GAATGC | 1 cut(s) | 1704 |
| BsnI | GGCC | 7 cut(s) | 230, 279, 1125, 1306, 1580, 1587, 2035 |
| Bsp1286I | GDGCHC | 3 cut(s) | 142, 1837, 1873 |
| Bsp1407I | TGTACA | 1 cut(s) | 1521 |
| Bsp143I | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2159 |
| Bsp68I | TCGCGA | 1 cut(s) | 296 |
| BspACI | CCGC | 6 cut(s) | 73, 171, 605, 674, 728, 1504 |
| BspANI | GGCC | 7 cut(s) | 230, 279, 1125, 1306, 1580, 1587, 2035 |
| BspCNI | CTCAG | 4 cut(s) | 198, 946, 1321, 2146 |
| BspDI | ATCGAT | 1 cut(s) | 243 |
| BspFNI | CGCG | 2 cut(s) | 296, 2026 |
| BspHI | TCATGA | 1 cut(s) | 1092 |
| BspLI | GGNNCC | 3 cut(s) | 333, 1655, 2171 |
| BspMAI | CTGCAG | 2 cut(s) | 1089, 2070 |
| BspMI | ACCTGC | 1 cut(s) | 1775 |
| BspPI | GGATC | 1 cut(s) | 216 |
| BspQI | GCTCTTC | 1 cut(s) | 1494 |
| BspT107I | GGYRCC | 2 cut(s) | 1653, 2169 |
| BsrBI | CCGCTC | 1 cut(s) | 1504 |
| BsrDI | GCAATG | 2 cut(s) | 1105, 2047 |
| BsrFI | RCCGGY | 1 cut(s) | 197 |
| BsrGI | TGTACA | 1 cut(s) | 1521 |
| BsrI | ACTGG | 3 cut(s) | 660, 973, 1806 |
| BssAI | RCCGGY | 1 cut(s) | 197 |
| BssECI | CCNNGG | 4 cut(s) | 358, 543, 1470, 1581 |
| BssMI | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| BssNI | GRCGYC | 1 cut(s) | 608 |
| BssT1I | CCWWGG | 2 cut(s) | 358, 1470 |
| Bst2UI | CCWGG | 2 cut(s) | 544, 1896 |
| Bst4CI | ACNGT | 7 cut(s) | 556, 842, 1043, 1133, 1542, 1875, 1923 |
| Bst6I | CTCTTC | 4 cut(s) | 74, 1117, 1494, 1752 |
| BstACI | GRCGYC | 1 cut(s) | 608 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1985 |
| BstAUI | TGTACA | 1 cut(s) | 1521 |
| BstBAI | YACGTR | 1 cut(s) | 1752 |
| BstC8I | GCNNGC | 4 cut(s) | 199, 990, 1506, 2073 |
| BstDEI | CTNAG | 6 cut(s) | 185, 414, 933, 1308, 2154, 2159 |
| BstDSI | CCRYGG | 1 cut(s) | 1581 |
| BstF5I | GGATG | 2 cut(s) | 1360, 1423 |
| BstFNI | CGCG | 2 cut(s) | 296, 2026 |
| BstKTI | GATC | 8 cut(s) | 211, 720, 775, 838, 1360, 1456, 1515, 2024 |
| BstMAI | GTCTC | 1 cut(s) | 1289 |
| BstMBI | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 171, 1593, 1910, 1985, 2023, 2032 |
| BstNI | CCWGG | 2 cut(s) | 544, 1896 |
| BstNSI | RCATGY | 2 cut(s) | 260, 2152 |
| BstSCI | CCNGG | 2 cut(s) | 542, 1894 |
| BstSFI | CTRYAG | 4 cut(s) | 234, 1085, 2012, 2066 |
| BstSLI | GKGCMC | 1 cut(s) | 1873 |
| BstUI | CGCG | 2 cut(s) | 296, 2026 |
| BstV1I | GCAGC | 4 cut(s) | 1913, 1918, 2052, 2080 |
| BstV2I | GAAGAC | 2 cut(s) | 438, 1257 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 328 |
| Bsu15I | ATCGAT | 1 cut(s) | 243 |
| BsuRI | GGCC | 7 cut(s) | 230, 279, 1125, 1306, 1580, 1587, 2035 |
| BsuTUI | ATCGAT | 1 cut(s) | 243 |
| BtgI | CCRYGG | 1 cut(s) | 1581 |
| BtsCI | GGATG | 2 cut(s) | 1360, 1423 |
| BtsIMutI | CAGTG | 2 cut(s) | 667, 1048 |
| BtuMI | TCGCGA | 1 cut(s) | 296 |
| BveI | ACCTGC | 1 cut(s) | 1775 |
| Cac8I | GCNNGC | 4 cut(s) | 199, 990, 1506, 2073 |
| CciI | TCATGA | 1 cut(s) | 1092 |
| Cfr10I | RCCGGY | 1 cut(s) | 197 |
| Cfr13I | GGNCC | 5 cut(s) | 657, 1123, 1304, 1585, 1892 |
| ClaI | ATCGAT | 1 cut(s) | 243 |
| CseI | GACGC | 2 cut(s) | 597, 2015 |
| Csp6I | GTAC | 4 cut(s) | 739, 1223, 1522, 1795 |
| CviAII | CATG | 9 cut(s) | 257, 1093, 1127, 1613, 1954, 1976, 2005, 2145, 2149 |
| CviQI | GTAC | 4 cut(s) | 739, 1223, 1522, 1795 |
| DdeI | CTNAG | 6 cut(s) | 185, 414, 933, 1308, 2154, 2159 |
| DpnI | GATC | 8 cut(s) | 210, 719, 774, 837, 1359, 1455, 1514, 2023 |
| DpnII | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| EaeI | YGGCCR | 1 cut(s) | 1578 |
| Eam1104I | CTCTTC | 4 cut(s) | 74, 1117, 1494, 1752 |
| EarI | CTCTTC | 4 cut(s) | 74, 1117, 1494, 1752 |
| Ecl136II | GAGCTC | 1 cut(s) | 140 |
| Eco130I | CCWWGG | 2 cut(s) | 358, 1470 |
| Eco147I | AGGCCT | 1 cut(s) | 279 |
| Eco24I | GRGCYC | 2 cut(s) | 142, 1837 |
| Eco47I | GGWCC | 2 cut(s) | 657, 1892 |
| Eco53kI | GAGCTC | 1 cut(s) | 140 |
| Eco72I | CACGTG | 1 cut(s) | 1752 |
| EcoICRI | GAGCTC | 1 cut(s) | 140 |
| EcoRI | GAATTC | 1 cut(s) | 1475 |
| EcoRII | CCWGG | 2 cut(s) | 542, 1894 |
| EcoT14I | CCWWGG | 2 cut(s) | 358, 1470 |
| EcoT22I | ATGCAT | 1 cut(s) | 2150 |
| EcoT38I | GRGCYC | 2 cut(s) | 142, 1837 |
| ErhI | CCWWGG | 2 cut(s) | 358, 1470 |
| FaeI | CATG | 9 cut(s) | 260, 1096, 1130, 1616, 1957, 1979, 2008, 2148, 2152 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1758, 1790, 2082, 2114 |
| FaqI | GGGAC | 2 cut(s) | 191, 424 |
| FatI | CATG | 9 cut(s) | 256, 1092, 1126, 1612, 1953, 1975, 2004, 2144, 2148 |
| FauI | CCCGC | 1 cut(s) | 1497 |
| FauNDI | CATATG | 1 cut(s) | 1200 |
| FbaI | TGATCA | 3 cut(s) | 772, 835, 1357 |
| FblI | GTMKAC | 1 cut(s) | 2029 |
| Fnu4HI | GCNGC | 6 cut(s) | 172, 606, 1902, 1932, 2066, 2069 |
| FokI | GGATG | 2 cut(s) | 1367, 1430 |
| FriOI | GRGCYC | 2 cut(s) | 142, 1837 |
| Fsp4HI | GCNGC | 6 cut(s) | 172, 606, 1902, 1932, 2066, 2069 |
| FspBI | CTAG | 6 cut(s) | 275, 303, 654, 783, 1716, 2037 |
| GluI | GCNGC | 6 cut(s) | 172, 606, 1902, 1932, 2066, 2069 |
| GsaI | CCCAGC | 1 cut(s) | 178 |
| GsuI | CTGGAG | 1 cut(s) | 1917 |
| HaeIII | GGCC | 7 cut(s) | 230, 279, 1125, 1306, 1580, 1587, 2035 |
| HapII | CCGG | 2 cut(s) | 198, 335 |
| HgaI | GACGC | 2 cut(s) | 597, 2015 |
| Hin1I | GRCGYC | 1 cut(s) | 608 |
| Hin1II | CATG | 9 cut(s) | 260, 1096, 1130, 1616, 1957, 1979, 2008, 2148, 2152 |
| HindIII | AAGCTT | 2 cut(s) | 986, 1212 |
| HpaII | CCGG | 2 cut(s) | 198, 335 |
| HphI | GGTGA | 5 cut(s) | 45, 410, 845, 1036, 2068 |
| Hpy166II | GTNNAC | 3 cut(s) | 1382, 1871, 2030 |
| Hpy188III | TCNNGA | 7 cut(s) | 111, 295, 935, 1093, 1334, 1368, 1640 |
| Hpy8I | GTNNAC | 3 cut(s) | 1382, 1871, 2030 |
| Hpy99I | CGWCG | 1 cut(s) | 197 |
| HpyAV | CCTTC | 3 cut(s) | 1616, 1644, 2047 |
| HpyCH4III | ACNGT | 7 cut(s) | 556, 842, 1043, 1133, 1542, 1875, 1923 |
| HpyCH4IV | ACGT | 1 cut(s) | 1751 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 171, 1593, 1910, 1985, 2023, 2032 |
| HpyF3I | CTNAG | 6 cut(s) | 185, 414, 933, 1308, 2154, 2159 |
| HpySE526I | ACGT | 1 cut(s) | 1751 |
| Hsp92I | GRCGYC | 1 cut(s) | 608 |
| Hsp92II | CATG | 9 cut(s) | 260, 1096, 1130, 1616, 1957, 1979, 2008, 2148, 2152 |
| KroI | GCCGGC | 1 cut(s) | 197 |
| KroNI | GCCGGC | 1 cut(s) | 199 |
| Ksp22I | TGATCA | 3 cut(s) | 772, 835, 1357 |
| Kzo9I | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| LguI | GCTCTTC | 1 cut(s) | 1494 |
| LmnI | GCTCC | 3 cut(s) | 465, 621, 1898 |
| Lsp1109I | GCAGC | 4 cut(s) | 1913, 1918, 2052, 2080 |
| LweI | GCATC | 5 cut(s) | 1742, 1900, 1939, 1975, 2083 |
| MaeI | CTAG | 6 cut(s) | 275, 303, 654, 783, 1716, 2037 |
| MaeII | ACGT | 1 cut(s) | 1751 |
| MaeIII | GTNAC | 3 cut(s) | 1045, 1438, 1667 |
| MalI | GATC | 8 cut(s) | 210, 719, 774, 837, 1359, 1455, 1514, 2023 |
| MbiI | CCGCTC | 1 cut(s) | 1504 |
| MboI | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| MfeI | CAATTG | 4 cut(s) | 585, 1361, 1812, 1935 |
| MhlI | GDGCHC | 3 cut(s) | 142, 1837, 1873 |
| MlyI | GAGTC | 1 cut(s) | 197 |
| Mph1103I | ATGCAT | 1 cut(s) | 2150 |
| MreI | CGCCGGCG | 1 cut(s) | 197 |
| MroNI | GCCGGC | 1 cut(s) | 197 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1770 |
| MseI | TTAA | 7 cut(s) | 404, 756, 1314, 1628, 1746, 2100, 2182 |
| MslI | CAYNNNNRTG | 2 cut(s) | 326, 1556 |
| MspI | CCGG | 2 cut(s) | 198, 335 |
| MspR9I | CCNGG | 2 cut(s) | 544, 1896 |
| MunI | CAATTG | 4 cut(s) | 585, 1361, 1812, 1935 |
| Mva1269I | GAATGC | 1 cut(s) | 1704 |
| MvaI | CCWGG | 2 cut(s) | 544, 1896 |
| MvnI | CGCG | 2 cut(s) | 296, 2026 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 171, 1593, 1910, 1985, 2023, 2032 |
| NaeI | GCCGGC | 1 cut(s) | 199 |
| NdeI | CATATG | 1 cut(s) | 1200 |
| NdeII | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| NgoMIV | GCCGGC | 1 cut(s) | 197 |
| NlaIII | CATG | 9 cut(s) | 260, 1096, 1130, 1616, 1957, 1979, 2008, 2148, 2152 |
| NlaIV | GGNNCC | 3 cut(s) | 333, 1655, 2171 |
| NmuCI | GTSAC | 1 cut(s) | 1667 |
| NruI | TCGCGA | 1 cut(s) | 296 |
| NsiI | ATGCAT | 1 cut(s) | 2150 |
| NspI | RCATGY | 2 cut(s) | 260, 2152 |
| PagI | TCATGA | 1 cut(s) | 1092 |
| PceI | AGGCCT | 1 cut(s) | 279 |
| PciSI | GCTCTTC | 1 cut(s) | 1494 |
| PctI | GAATGC | 1 cut(s) | 1704 |
| PdiI | GCCGGC | 1 cut(s) | 199 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1770 |
| PkrI | GCNGC | 6 cut(s) | 173, 607, 1903, 1933, 2067, 2070 |
| PleI | GAGTC | 1 cut(s) | 197 |
| PmaCI | CACGTG | 1 cut(s) | 1752 |
| PmlI | CACGTG | 1 cut(s) | 1752 |
| PpsI | GAGTC | 1 cut(s) | 197 |
| Ppu21I | YACGTR | 1 cut(s) | 1752 |
| Psp124BI | GAGCTC | 1 cut(s) | 142 |
| Psp6I | CCWGG | 2 cut(s) | 542, 1894 |
| PspCI | CACGTG | 1 cut(s) | 1752 |
| PspFI | CCCAGC | 1 cut(s) | 174 |
| PspGI | CCWGG | 2 cut(s) | 542, 1894 |
| PspN4I | GGNNCC | 3 cut(s) | 333, 1655, 2171 |
| PspPI | GGNCC | 5 cut(s) | 657, 1123, 1304, 1585, 1892 |
| PstI | CTGCAG | 2 cut(s) | 1089, 2070 |
| RruI | TCGCGA | 1 cut(s) | 296 |
| RsaI | GTAC | 4 cut(s) | 740, 1224, 1523, 1796 |
| RsaNI | GTAC | 4 cut(s) | 739, 1223, 1522, 1795 |
| RseI | CAYNNNNRTG | 2 cut(s) | 326, 1556 |
| SacI | GAGCTC | 1 cut(s) | 142 |
| SapI | GCTCTTC | 1 cut(s) | 1494 |
| SaqAI | TTAA | 7 cut(s) | 404, 756, 1314, 1628, 1746, 2100, 2182 |
| SatI | GCNGC | 6 cut(s) | 172, 606, 1902, 1932, 2066, 2069 |
| Sau3AI | GATC | 8 cut(s) | 208, 717, 772, 835, 1357, 1453, 1512, 2021 |
| Sau96I | GGNCC | 5 cut(s) | 657, 1123, 1304, 1585, 1892 |
| SchI | GAGTC | 1 cut(s) | 197 |
| ScrFI | CCNGG | 2 cut(s) | 544, 1896 |
| SduI | GDGCHC | 3 cut(s) | 142, 1837, 1873 |
| SfaNI | GCATC | 5 cut(s) | 1742, 1900, 1939, 1975, 2083 |
| SfcI | CTRYAG | 4 cut(s) | 234, 1085, 2012, 2066 |
| SgrAI | CRCCGGYG | 1 cut(s) | 197 |
| SinI | GGWCC | 2 cut(s) | 657, 1892 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 326, 1556 |
| SmlI | CTYRAG | 2 cut(s) | 145, 1238 |
| SmoI | CTYRAG | 2 cut(s) | 145, 1238 |
| SpeI | ACTAGT | 1 cut(s) | 1715 |
| SseBI | AGGCCT | 1 cut(s) | 279 |
| SsiI | CCGC | 6 cut(s) | 73, 171, 605, 674, 728, 1504 |
| SspMI | CTAG | 6 cut(s) | 275, 303, 654, 783, 1716, 2037 |
| SstI | GAGCTC | 1 cut(s) | 142 |
| StuI | AGGCCT | 1 cut(s) | 279 |
| StyD4I | CCNGG | 2 cut(s) | 542, 1894 |
| StyI | CCWWGG | 2 cut(s) | 358, 1470 |
| TaaI | ACNGT | 7 cut(s) | 556, 842, 1043, 1133, 1542, 1875, 1923 |
| TaiI | ACGT | 1 cut(s) | 1754 |
| TaqI | TCGA | 2 cut(s) | 243, 1947 |
| TaqII | GACCGA | 1 cut(s) | 931 |
| TatI | WGTACW | 3 cut(s) | 738, 1521, 1794 |
| TauI | GCSGC | 2 cut(s) | 174, 608 |
| Tru1I | TTAA | 7 cut(s) | 404, 756, 1314, 1628, 1746, 2100, 2182 |
| Tru9I | TTAA | 7 cut(s) | 404, 756, 1314, 1628, 1746, 2100, 2182 |
| TscAI | CASTG | 2 cut(s) | 667, 1048 |
| TseFI | GTSAC | 1 cut(s) | 1667 |
| TseI | GCWGC | 4 cut(s) | 1901, 1931, 2065, 2068 |
| Tsp45I | GTSAC | 1 cut(s) | 1667 |
| TspGWI | ACGGA | 3 cut(s) | 121, 450, 1856 |
| TspRI | CASTG | 2 cut(s) | 667, 1048 |
| VneI | GTGCAC | 1 cut(s) | 1869 |
| VpaK11BI | GGWCC | 2 cut(s) | 657, 1892 |
| XapI | RAATTY | 3 cut(s) | 919, 1342, 1475 |
| XceI | RCATGY | 2 cut(s) | 260, 2152 |
| XmiI | GTMKAC | 1 cut(s) | 2029 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1770 |
| XspI | CTAG | 6 cut(s) | 275, 303, 654, 783, 1716, 2037 |
| Zsp2I | ATGCAT | 1 cut(s) | 2150 |