pycom10g24870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010537
Physical Location & Seq
Forward (+)
26599961 .. 26601280
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24870.3

Sequence Viewer

Length: 1068 bp
ATGGATCATAATAATCACACTGAAACGTGGGAATCCCCTGAAAGTCTACTTGATTCTGTAGATTTCGGTGTCAGAACAAAACAGATGGACAATAAAGCTTCCCTTGTACAAGAACAAATGCTGAAGCAATCTAATGGGGTTTACAGACCACCTAATATCAGGGGGAAAAATGTGAATGGAACTTATGAGTCTCATAGTAGAGAAGAGCATGTAAATCGCAATGACGATTCTCATTTGGCTGATCACCTGTCTGGACATGAGAGTGAACTACCTTCCCTTTCATCTTCGTTGGTTTCGGATGATATATTGTTTGATAGGTATGTTACGGAAGCTAATGATCTTCTAAAACAAGCCAAGGAATTTGTAAGGGTCGGACACAATGAAGAGCGTGCAGAGATAGTATTGTACAGGTCAGCCAAGTTGCTGTCCAAAGCCATATCTATGAAGCCCATGAGTTTGCTGGCAGTGGGCCAGTTGGGCAACACTTGCCTTCTTCATGGAGAATTAAAATTAAGGATTAGTCGCGAGTTGAGAATGCATCTTTCGAGAAGTGACCCCTTATCTGTTGAGAAATGGATTAGAATTCAGGATAAAGTCACCAGTAAAGATGACATTGCATCACTCCTCATTATTGTGTGTGAAGAGTGTGAGGAACTTCTTGTGGAAGCAGGCAGAAGGTATAGAATGGCGTTATCAATTGACGGGAATGATACGAGAGCCCTATATAATTGGGGTCTTGCTCTCACCTTCCGTGCGCAGTTGATTGCAGATATCGGGCCGGAAGCAGCTTTTGATGCTGACGAACTGTTCTTGGCTGCAATCGATAAGTTCAATGCCATGATGTCAAAGGGAAATGTTTATGCACCAAATGCTCTGCTTCGATGGGGTGTGGCACTACAGCAAAGATCCCGCCTACGGCCTAGCAATGGTAAAGAGAAGGTGAAGTTACTACAGCAGGCAAAGAGGCTGTATGAAGATGCACTCAAGATGGATTCGAACAATGTCCACGTGAGAGAAGCCTTATCATCGTGTGCGTCGGAGCTTGGCAGTAGGCGACTTTACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

40.13

Weight (kDa)

6.29

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 756
AccI GTMKAC 1 cut(s) 46
AccII CGCG 1 cut(s) 525
AciI CCGC 1 cut(s) 910
AclWI GGATC 2 cut(s) 12, 900
AcsI RAATTY 2 cut(s) 359, 582
AcuI CTGAAG 1 cut(s) 143
AcvI CACGTG 1 cut(s) 1009
AfaI GTAC 2 cut(s) 108, 407
AfiI CCNNNNNNNGG 2 cut(s) 915, 926
AgsI TTSAA 1 cut(s) 832
AluBI AGCT 4 cut(s) 98, 332, 788, 1042
AluI AGCT 4 cut(s) 98, 332, 788, 1042
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 2 cut(s) 12, 900
AoxI GGCC 3 cut(s) 469, 776, 917
ApeKI GCWGC 2 cut(s) 785, 815
ApoI RAATTY 2 cut(s) 359, 582
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 2 cut(s) 469, 776
AsuHPI GGTGA 4 cut(s) 236, 589, 736, 952
AsuII TTCGAA 1 cut(s) 995
BanII GRGCYC 1 cut(s) 721
BbrPI CACGTG 1 cut(s) 1009
BbvI GCAGC 2 cut(s) 797, 802
BccI CCATC 3 cut(s) 79, 876, 982
BceAI ACGGC 1 cut(s) 932
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BclI TGATCA 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 3 cut(s) 57, 896, 950
BglI GCCNNNNNGGC 1 cut(s) 477
BisI GCNGC 2 cut(s) 786, 816
BlsI GCNGC 2 cut(s) 787, 817
BmgT120I GGNCC 2 cut(s) 469, 776
BmsI GCATC 4 cut(s) 547, 626, 784, 967
Bpu14I TTCGAA 1 cut(s) 995
BpuEI CTTGAG 1 cut(s) 968
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 1 cut(s) 1009
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 915, 926
Bse1I ACTGG 2 cut(s) 472, 600
Bse3DI GCAATG 3 cut(s) 226, 612, 931
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 915, 926
BseMI GCAATG 3 cut(s) 226, 612, 931
BseNI ACTGG 2 cut(s) 472, 600
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 2 cut(s) 797, 802
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 3 cut(s) 471, 778, 919
BshVI ATCGAT 1 cut(s) 822
BsiSI CCGG 1 cut(s) 779
BslI CCNNNNNNNGG 2 cut(s) 915, 926
BsmAI GTCTC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 3 cut(s) 471, 778, 919
Bsp119I TTCGAA 1 cut(s) 995
Bsp1286I GDGCHC 1 cut(s) 721
Bsp1407I TGTACA 2 cut(s) 106, 405
Bsp143I GATC 4 cut(s) 4, 241, 337, 905
Bsp68I TCGCGA 1 cut(s) 525
BspACI CCGC 1 cut(s) 910
BspANI GGCC 3 cut(s) 471, 778, 919
BspDI ATCGAT 1 cut(s) 822
BspFNI CGCG 1 cut(s) 525
BspPI GGATC 2 cut(s) 12, 900
BspQI GCTCTTC 2 cut(s) 198, 378
BspT104I TTCGAA 1 cut(s) 995
BsrDI GCAATG 3 cut(s) 226, 612, 931
BsrGI TGTACA 2 cut(s) 106, 405
BsrI ACTGG 2 cut(s) 472, 600
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 4 cut(s) 4, 241, 337, 905
BssT1I CCWWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 807
Bst6I CTCTTC 3 cut(s) 198, 378, 636
BstAPI GCANNNNNTGC 2 cut(s) 486, 869
BstAUI TGTACA 2 cut(s) 106, 405
BstBAI YACGTR 1 cut(s) 1009
BstBI TTCGAA 1 cut(s) 995
BstC8I GCNNGC 4 cut(s) 390, 462, 670, 957
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 1 cut(s) 525
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 4 cut(s) 7, 244, 340, 908
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 4 cut(s) 4, 241, 337, 905
BstMWI GCNNNNNNNGC 4 cut(s) 477, 486, 794, 869
BstNSI RCATGY 1 cut(s) 212
BstSFI CTRYAG 3 cut(s) 57, 896, 950
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 797, 802
BstX2I RGATCY 1 cut(s) 905
BstYI RGATCY 1 cut(s) 905
Bsu15I ATCGAT 1 cut(s) 822
BsuRI GGCC 3 cut(s) 471, 778, 919
BsuTUI ATCGAT 1 cut(s) 822
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 2 cut(s) 18, 471
BtuMI TCGCGA 1 cut(s) 525
Cac8I GCNNGC 4 cut(s) 390, 462, 670, 957
CfoI GCGC 1 cut(s) 757
Cfr13I GGNCC 2 cut(s) 469, 776
ClaI ATCGAT 1 cut(s) 822
CseI GACGC 1 cut(s) 1023
Csp6I GTAC 2 cut(s) 107, 406
CviAII CATG 5 cut(s) 209, 257, 451, 497, 838
CviQI GTAC 2 cut(s) 107, 406
DpnI GATC 4 cut(s) 6, 243, 339, 907
DpnII GATC 4 cut(s) 4, 241, 337, 905
Eam1104I CTCTTC 3 cut(s) 198, 378, 636
EarI CTCTTC 3 cut(s) 198, 378, 636
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 721
Eco32I GATATC 1 cut(s) 772
Eco57I CTGAAG 1 cut(s) 143
Eco72I CACGTG 1 cut(s) 1009
EcoRI GAATTC 1 cut(s) 582
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 5 cut(s) 212, 260, 454, 500, 841
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FatI CATG 5 cut(s) 208, 256, 450, 496, 837
FauI CCCGC 1 cut(s) 917
FbaI TGATCA 1 cut(s) 241
FblI GTMKAC 1 cut(s) 46
Fnu4HI GCNGC 2 cut(s) 786, 816
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 2 cut(s) 786, 816
FspBI CTAG 1 cut(s) 921
FspI TGCGCA 1 cut(s) 756
GlaI GCGC 1 cut(s) 756
GluI GCNGC 2 cut(s) 786, 816
HaeIII GGCC 3 cut(s) 471, 778, 919
HapII CCGG 1 cut(s) 779
HgaI GACGC 1 cut(s) 1023
HhaI GCGC 1 cut(s) 757
Hin1II CATG 5 cut(s) 212, 260, 454, 500, 841
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 5 cut(s) 32, 53, 188, 227, 992
HpaII CCGG 1 cut(s) 779
HphI GGTGA 4 cut(s) 236, 589, 736, 952
Hpy166II GTNNAC 4 cut(s) 47, 142, 266, 1006
Hpy188I TCNGA 4 cut(s) 74, 298, 374, 1039
Hpy188III TCNNGA 5 cut(s) 252, 524, 546, 587, 985
Hpy8I GTNNAC 4 cut(s) 47, 142, 266, 1006
Hpy99I CGWCG 1 cut(s) 1039
HpyAV CCTTC 5 cut(s) 282, 500, 669, 757, 931
HpyCH4III ACNGT 1 cut(s) 807
HpyCH4IV ACGT 2 cut(s) 26, 1008
HpyCH4V TGCA 7 cut(s) 392, 538, 617, 767, 818, 863, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 477, 486, 794, 869
HpySE526I ACGT 2 cut(s) 26, 1008
Hsp92II CATG 5 cut(s) 212, 260, 454, 500, 841
HspAI GCGC 1 cut(s) 755
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 4 cut(s) 4, 241, 337, 905
LguI GCTCTTC 2 cut(s) 198, 378
LmnI GCTCC 1 cut(s) 1039
Lsp1109I GCAGC 2 cut(s) 797, 802
LweI GCATC 4 cut(s) 547, 626, 784, 967
MaeI CTAG 1 cut(s) 921
MaeII ACGT 2 cut(s) 26, 1008
MaeIII GTNAC 4 cut(s) 322, 551, 595, 945
MalI GATC 4 cut(s) 6, 243, 339, 907
MboI GATC 4 cut(s) 4, 241, 337, 905
MboII GAAGA 7 cut(s) 215, 276, 332, 395, 485, 653, 986
MfeI CAATTG 1 cut(s) 696
MflI RGATCY 1 cut(s) 905
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 6 cut(s) 359, 503, 509, 582, 696, 727
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 352, 1017
MnlI CCTC 3 cut(s) 635, 643, 957
Mph1103I ATGCAT 1 cut(s) 540
MseI TTAA 3 cut(s) 506, 512, 1066
MslI CAYNNNNRTG 3 cut(s) 261, 440, 632
MspI CCGG 1 cut(s) 779
MunI CAATTG 1 cut(s) 696
Mva1269I GAATGC 1 cut(s) 540
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 4 cut(s) 477, 486, 794, 869
NdeII GATC 4 cut(s) 4, 241, 337, 905
NlaIII CATG 5 cut(s) 212, 260, 454, 500, 841
NmuCI GTSAC 2 cut(s) 551, 595
NruI TCGCGA 1 cut(s) 525
NsbI TGCGCA 1 cut(s) 756
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 212
NspV TTCGAA 1 cut(s) 995
PciSI GCTCTTC 2 cut(s) 198, 378
PctI GAATGC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 32, 53, 227, 992
PkrI GCNGC 2 cut(s) 787, 817
PleI GAGTC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 1009
PmlI CACGTG 1 cut(s) 1009
PpsI GAGTC 1 cut(s) 196
Ppu21I YACGTR 1 cut(s) 1009
PspCI CACGTG 1 cut(s) 1009
PspPI GGNCC 2 cut(s) 469, 776
PsuI RGATCY 1 cut(s) 905
RruI TCGCGA 1 cut(s) 525
RsaI GTAC 2 cut(s) 108, 407
RsaNI GTAC 2 cut(s) 107, 406
RseI CAYNNNNRTG 3 cut(s) 261, 440, 632
SapI GCTCTTC 2 cut(s) 198, 378
SaqAI TTAA 3 cut(s) 506, 512, 1066
SatI GCNGC 2 cut(s) 786, 816
Sau3AI GATC 4 cut(s) 4, 241, 337, 905
Sau96I GGNCC 2 cut(s) 469, 776
SchI GAGTC 1 cut(s) 197
SduI GDGCHC 1 cut(s) 721
SfaNI GCATC 4 cut(s) 547, 626, 784, 967
SfcI CTRYAG 3 cut(s) 57, 896, 950
SfuI TTCGAA 1 cut(s) 995
SmiMI CAYNNNNRTG 3 cut(s) 261, 440, 632
SmlI CTYRAG 1 cut(s) 983
SmoI CTYRAG 1 cut(s) 983
Sse9I AATT 6 cut(s) 359, 503, 509, 582, 696, 727
SsiI CCGC 1 cut(s) 910
SspMI CTAG 1 cut(s) 921
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 807
TaiI ACGT 2 cut(s) 29, 1011
TaqI TCGA 4 cut(s) 545, 822, 880, 995
TasI AATT 6 cut(s) 359, 503, 509, 582, 696, 727
TatI WGTACW 2 cut(s) 106, 405
TfiI GAWTC 4 cut(s) 32, 53, 227, 992
Tru1I TTAA 3 cut(s) 506, 512, 1066
Tru9I TTAA 3 cut(s) 506, 512, 1066
TscAI CASTG 2 cut(s) 25, 471
TseFI GTSAC 2 cut(s) 551, 595
TseI GCWGC 2 cut(s) 785, 815
Tsp45I GTSAC 2 cut(s) 551, 595
TspDTI ATGAA 5 cut(s) 270, 396, 458, 485, 987
TspGWI ACGGA 2 cut(s) 341, 740
TspRI CASTG 2 cut(s) 25, 471
XapI RAATTY 2 cut(s) 359, 582
XceI RCATGY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 46
XspI CTAG 1 cut(s) 921
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.