FvH4_3g16360

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018399
Physical Location & Seq
Reverse (-)
10310074 .. 10312847
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16360.t1

Sequence Viewer

Length: 651 bp
ATGGCGGATGAGGTGGTGCTGCTGGGATTCTTACCGAGCCCATTTGCGGCGAGGTTGAAGATTGCTTTGGCTGAGAAGGGGGTCGAGTATGAGTACAAGGAGGAGGACTTGAGGAGCAAGAGTCCACTACTTGTGCAGATGAACCCGGTTCACATGAAGGTTCCGGTTCTCATTCACAATGGCAAACCGGTCTGCGAGTCCCTCATTGCGCTGCAGTACATCGATGAGGTTTGGAAAGATAAAGCTCCACTGTTGCCCTCCGATCCTGGCCTTCGAGCCCAAGCCATGTTCTGGGCAGACTTCATTGACAAGAAGGAGAAGGTATGGGCAGCCAAAGAAGAAGAACATGAGGCAGCGAAGGAGTTTTTTTATTGCATTGGGGTGTTAGAAGGAGAGCTCGGAGACAAAGCTTTCTTTGGGGGTGATACTCTTGGGTTTGTGGATGTGGCAGTTATACCATTCTATAGCTGGCTTTCTGTGTTTGAAAAATATGGAAATTGCAGTGTAGAAAACTCGCATCCAAAGTTCACTGCCTGGACTAAGAGGTGCATGGAGAAGGAGAGTGTCTCCAAGTCTCTTCCTGATCAGCAACTGGTCTATGACTTCAAACTTGGGGGCATAATAAAGTTACCAATCCATCAAGACCTGTAA

Protein Analysis

217

Amino Acids

24.46

Weight (kDa)

5.22

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 2e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 76 7.6e-12 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 1.9e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 113 - 186 4.6e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 118 - 182 1.3e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g16360
malus_domestica MD05G1211300.v1.1
rosa_chinensis RchiOBHm_Chr5g0027401
rosa_laevigata RLG00000033017
rosa_multiflora Rmu_co8135500.1_g000001 Rmu_sc0007802.1_g000014
rosa_roxburghii Rroxscaffold_1G00052120
rosa_samantha Rh5AG192900 Rh5DG192300
rosa_wichuraiana Rw5G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 2 cut(s) 5, 47
AclWI GGATC 1 cut(s) 257
AfaI GTAC 2 cut(s) 95, 218
AfiI CCNNNNNNNGG 2 cut(s) 46, 291
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 3 cut(s) 58, 485, 607
AjnI CCWGG 2 cut(s) 265, 533
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 4 cut(s) 245, 397, 410, 468
AluI AGCT 4 cut(s) 245, 397, 410, 468
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 396, 571, 579
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 592
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 4 cut(s) 19, 211, 329, 353
AsiGI ACCGGT 1 cut(s) 187
AspLEI GCGC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 434
BanII GRGCYC 3 cut(s) 41, 280, 399
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 4 cut(s) 6, 198, 341, 365
BccI CCATC 1 cut(s) 645
BciT130I CCWGG 2 cut(s) 267, 535
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 3 cut(s) 396, 571, 579
BfmI CTRYAG 2 cut(s) 212, 463
BisI GCNGC 5 cut(s) 20, 48, 212, 330, 354
BlsI GCNGC 5 cut(s) 21, 49, 213, 331, 355
Bme1390I CCNGG 3 cut(s) 146, 267, 535
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 3 cut(s) 146, 267, 535
BmsI GCATC 1 cut(s) 526
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpuEI CTTGAG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 163, 187
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 291
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 2 cut(s) 267, 535
BseCI ATCGAT 1 cut(s) 222
BseGI GGATG 3 cut(s) 13, 448, 517
BseLI CCNNNNNNNGG 2 cut(s) 46, 291
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 2 cut(s) 116, 127
BseXI GCAGC 4 cut(s) 6, 198, 341, 365
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 1 cut(s) 270
BshTI ACCGGT 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 146, 164, 188
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 46, 291
BsmAI GTCTC 3 cut(s) 396, 571, 579
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 270
Bsp1286I GDGCHC 3 cut(s) 41, 280, 399
Bsp143I GATC 2 cut(s) 262, 583
BspACI CCGC 2 cut(s) 5, 47
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 162
BspMAI CTGCAG 1 cut(s) 216
BspPI GGATC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 187
BssMI GATC 2 cut(s) 262, 583
Bst2UI CCWGG 2 cut(s) 267, 535
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 72, 540
BstF5I GGATG 3 cut(s) 13, 448, 517
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 2 cut(s) 265, 586
BstMAI GTCTC 3 cut(s) 396, 571, 579
BstMBI GATC 2 cut(s) 262, 583
BstNI CCWGG 2 cut(s) 267, 535
BstSCI CCNGG 3 cut(s) 144, 265, 533
BstSFI CTRYAG 2 cut(s) 212, 463
BstV1I GCAGC 4 cut(s) 6, 198, 341, 365
Bsu15I ATCGAT 1 cut(s) 222
BsuRI GGCC 1 cut(s) 270
BsuTUI ATCGAT 1 cut(s) 222
BtsCI GGATG 3 cut(s) 13, 448, 517
BtsI GCAGTG 2 cut(s) 508, 528
BtsIMutI CAGTG 3 cut(s) 248, 508, 528
Cac8I GCNNGC 1 cut(s) 470
CaiI CAGNNNCTG 1 cut(s) 592
CfoI GCGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 222
Csp6I GTAC 2 cut(s) 94, 217
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 4 cut(s) 154, 286, 347, 550
CviQI GTAC 2 cut(s) 94, 217
DdeI CTNAG 2 cut(s) 72, 540
DpnI GATC 2 cut(s) 264, 585
DpnII GATC 2 cut(s) 262, 583
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 397
Eco24I GRGCYC 3 cut(s) 41, 280, 399
Eco53kI GAGCTC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 265, 533
EcoT38I GRGCYC 3 cut(s) 41, 280, 399
FaeI CATG 4 cut(s) 157, 289, 350, 553
FaqI GGGAC 1 cut(s) 184
FatI CATG 4 cut(s) 153, 285, 346, 549
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 5 cut(s) 20, 48, 212, 330, 354
FokI GGATG 3 cut(s) 20, 455, 504
FriOI GRGCYC 3 cut(s) 41, 280, 399
Fsp4HI GCNGC 5 cut(s) 20, 48, 212, 330, 354
GlaI GCGC 1 cut(s) 210
GluI GCNGC 5 cut(s) 20, 48, 212, 330, 354
GsaI CCCAGC 1 cut(s) 26
HaeIII GGCC 1 cut(s) 270
HapII CCGG 3 cut(s) 146, 164, 188
HhaI GCGC 1 cut(s) 211
Hin1II CATG 4 cut(s) 157, 289, 350, 553
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 3 cut(s) 27, 121, 197
HpaII CCGG 3 cut(s) 146, 164, 188
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 3 cut(s) 125, 151, 528
Hpy188I TCNGA 2 cut(s) 262, 401
Hpy188III TCNNGA 2 cut(s) 581, 641
Hpy8I GTNNAC 3 cut(s) 125, 151, 528
HpyAV CCTTC 8 cut(s) 70, 151, 281, 307, 313, 352, 383, 550
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 5 cut(s) 136, 214, 375, 501, 549
HpyF3I CTNAG 2 cut(s) 72, 540
Hsp92II CATG 4 cut(s) 157, 289, 350, 553
HspAI GCGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 2 cut(s) 262, 583
LmnI GCTCC 2 cut(s) 114, 250
Lsp1109I GCAGC 4 cut(s) 6, 198, 341, 365
LweI GCATC 1 cut(s) 526
MaeIII GTNAC 1 cut(s) 627
MalI GATC 2 cut(s) 264, 585
MboI GATC 2 cut(s) 262, 583
MboII GAAGA 4 cut(s) 70, 350, 353, 569
MhlI GDGCHC 3 cut(s) 41, 280, 399
MluCI AATT 1 cut(s) 496
MlyI GAGTC 2 cut(s) 130, 206
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 3 cut(s) 146, 164, 188
MspR9I CCNGG 3 cut(s) 146, 267, 535
MvaI CCWGG 2 cut(s) 267, 535
NciI CCSGG 1 cut(s) 146
NdeII GATC 2 cut(s) 262, 583
NlaIII CATG 4 cut(s) 157, 289, 350, 553
NlaIV GGNNCC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 291
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 5 cut(s) 21, 49, 213, 331, 355
PleI GAGTC 2 cut(s) 129, 205
PpsI GAGTC 2 cut(s) 129, 205
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 265, 533
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 2 cut(s) 265, 533
PspN4I GGNNCC 1 cut(s) 162
PstI CTGCAG 1 cut(s) 216
PstNI CAGNNNCTG 1 cut(s) 592
RsaI GTAC 2 cut(s) 95, 218
RsaNI GTAC 2 cut(s) 94, 217
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 399
SatI GCNGC 5 cut(s) 20, 48, 212, 330, 354
Sau3AI GATC 2 cut(s) 262, 583
SchI GAGTC 2 cut(s) 130, 206
ScrFI CCNGG 3 cut(s) 146, 267, 535
SduI GDGCHC 3 cut(s) 41, 280, 399
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 2 cut(s) 212, 463
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 496
SsiI CCGC 2 cut(s) 5, 47
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 144, 265, 533
TaaI ACNGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 84, 222, 274
TasI AATT 1 cut(s) 496
TatI WGTACW 2 cut(s) 93, 216
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 27
TscAI CASTG 3 cut(s) 255, 508, 535
TseI GCWGC 4 cut(s) 19, 211, 329, 353
TspDTI ATGAA 3 cut(s) 155, 170, 292
TspRI CASTG 3 cut(s) 255, 508, 535
Van91I CCANNNNNTGG 1 cut(s) 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.