RLG00000033017

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018399
Physical Location & Seq
Reverse (-)
22299949 .. 22301054
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033017

Sequence Viewer

Length: 654 bp
ATGGCGGATGAGGTGGTGTTGTTGGGATTCTTGCCGAGCCCATTTGCGGCGAGGTTGAAGATTGCACTGGCTGAGAAGGGGGTCGAGTATGAGTACAAGGAGGAGGACTTGAGGAGCAAGAGTCCACTACTTGTGCAGATGAACCCGGTTCGCAAGAAAGTTCCGGTTCTCATTCACAATGGCAAACCGATTTGTGAGTCCCTTATTGCGCTTCAGTACATCGATGAGGTTTGGAACGATAAGGCTCCACTGTTGCCCTCCGATCCTGGCCTTCGGGCCCAAGCCAGGTTCTGGGCAGACTTTATTGACAAGAAGGAGATGATATGGACAGCCAAAGAAGAAGAACATGAGGCAGCGAAGGAATTTTTTGATTGCATTGGGGTGTTAGAAGGACAGCTTGGAGACAAAGCTTTCTTTGGGGGTGAGACTCTTGGGTTTGTGGATGTGGCAGTTATTCCATTCTATAGCTGGCTTTCTGTGTTTGAAAAATATGGAAATTGCAGTGTAGAAGCCAAGCATCCAAAGTTCACTGCATGGACTAAGAGGTGCATGGAGAAGGAGAGTGTGTCAAAGTCTCTTCCTGATCAGCAAAGGGTCTATGACTTTAAGGTTGGGGGCATATTAACCAAGTTACCAATCGATCAAGACCTGTAA

Protein Analysis

218

Amino Acids

24.62

Weight (kDa)

5.26

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 2.2e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 76 8.1e-13 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 3.3e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 112 - 186 1.4e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 117 - 182 1e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g16360
malus_domestica MD05G1211300.v1.1
rosa_chinensis RchiOBHm_Chr5g0027401
rosa_laevigata RLG00000033017
rosa_multiflora Rmu_co8135500.1_g000001 Rmu_sc0007802.1_g000014
rosa_roxburghii Rroxscaffold_1G00052120
rosa_samantha Rh5AG192900 Rh5DG192300
rosa_wichuraiana Rw5G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 2 cut(s) 5, 47
AclWI GGATC 1 cut(s) 257
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 2 cut(s) 95, 218
AfiI CCNNNNNNNGG 3 cut(s) 46, 285, 291
AgsI TTSAA 2 cut(s) 58, 485
AjnI CCWGG 2 cut(s) 265, 284
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 3 cut(s) 397, 410, 468
AluI AGCT 3 cut(s) 397, 410, 468
Alw26I GTCTC 3 cut(s) 396, 419, 579
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 2 cut(s) 268, 276
ApaI GGGCCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 362
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 276, 277
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 280
BanII GRGCYC 2 cut(s) 41, 280
BbvI GCAGC 1 cut(s) 365
BciT130I CCWGG 2 cut(s) 267, 286
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 3 cut(s) 396, 419, 579
BfmI CTRYAG 1 cut(s) 463
BisI GCNGC 2 cut(s) 48, 354
BlsI GCNGC 2 cut(s) 49, 355
Bme1390I CCNGG 3 cut(s) 146, 267, 286
BmgT120I GGNCC 2 cut(s) 276, 277
BmiI GGNNCC 2 cut(s) 246, 278
BmrFI CCNGG 3 cut(s) 146, 267, 286
BmsI GCATC 1 cut(s) 526
BpuEI CTTGAG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 146
Bsa29I ATCGAT 2 cut(s) 222, 639
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 4 cut(s) 106, 136, 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 285, 291
Bse1I ACTGG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 267, 286
BseCI ATCGAT 2 cut(s) 222, 639
BseGI GGATG 3 cut(s) 13, 448, 517
BseLI CCNNNNNNNGG 3 cut(s) 46, 285, 291
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 116, 127
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 2 cut(s) 270, 278
BshVI ATCGAT 2 cut(s) 222, 639
BsiSI CCGG 2 cut(s) 146, 164
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 46, 285, 291
BsmAI GTCTC 3 cut(s) 396, 419, 579
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 2 cut(s) 270, 278
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 41, 280
Bsp143I GATC 3 cut(s) 262, 583, 640
BspACI CCGC 2 cut(s) 5, 47
BspANI GGCC 2 cut(s) 270, 278
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 2 cut(s) 222, 639
BspLI GGNNCC 2 cut(s) 246, 278
BspPI GGATC 1 cut(s) 257
BsrI ACTGG 1 cut(s) 72
BssMI GATC 3 cut(s) 262, 583, 640
Bst2UI CCWGG 2 cut(s) 267, 286
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 72, 540
BstF5I GGATG 3 cut(s) 13, 448, 517
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 3 cut(s) 265, 586, 643
BstMAI GTCTC 3 cut(s) 396, 419, 579
BstMBI GATC 3 cut(s) 262, 583, 640
BstNI CCWGG 2 cut(s) 267, 286
BstSCI CCNGG 3 cut(s) 144, 265, 284
BstSFI CTRYAG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 365
Bsu15I ATCGAT 2 cut(s) 222, 639
BsuRI GGCC 2 cut(s) 270, 278
BsuTUI ATCGAT 2 cut(s) 222, 639
BtsCI GGATG 3 cut(s) 13, 448, 517
BtsI GCAGTG 2 cut(s) 508, 528
BtsIMutI CAGTG 4 cut(s) 65, 248, 508, 528
Cac8I GCNNGC 1 cut(s) 470
CaiI CAGNNNCTG 1 cut(s) 291
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 276, 277
ClaI ATCGAT 2 cut(s) 222, 639
Csp6I GTAC 2 cut(s) 94, 217
CviAII CATG 3 cut(s) 347, 534, 550
CviQI GTAC 2 cut(s) 94, 217
DdeI CTNAG 2 cut(s) 72, 540
DpnI GATC 3 cut(s) 264, 585, 642
DpnII GATC 3 cut(s) 262, 583, 640
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 2 cut(s) 41, 280
Eco57I CTGAAG 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 265, 284
EcoT38I GRGCYC 2 cut(s) 41, 280
FaeI CATG 3 cut(s) 350, 537, 553
FaiI YATR 9 cut(s) 90, 325, 348, 465, 492, 535, 551, 600, 620
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 346, 533, 549
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 2 cut(s) 48, 354
FokI GGATG 3 cut(s) 20, 455, 504
FriOI GRGCYC 2 cut(s) 41, 280
Fsp4HI GCNGC 2 cut(s) 48, 354
GlaI GCGC 1 cut(s) 210
GluI GCNGC 2 cut(s) 48, 354
HaeIII GGCC 2 cut(s) 270, 278
HapII CCGG 2 cut(s) 146, 164
HhaI GCGC 1 cut(s) 211
Hin1II CATG 3 cut(s) 350, 537, 553
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 4 cut(s) 27, 121, 197, 427
HpaII CCGG 2 cut(s) 146, 164
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 125, 528
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 581, 644
Hpy8I GTNNAC 2 cut(s) 125, 528
HpyAV CCTTC 6 cut(s) 70, 281, 307, 352, 383, 550
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 6 cut(s) 65, 136, 375, 501, 533, 549
HpyF3I CTNAG 2 cut(s) 72, 540
Hsp92II CATG 3 cut(s) 350, 537, 553
HspAI GCGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 3 cut(s) 262, 583, 640
LmnI GCTCC 2 cut(s) 114, 250
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 1 cut(s) 526
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 264, 585, 642
MboI GATC 3 cut(s) 262, 583, 640
MboII GAAGA 4 cut(s) 70, 350, 353, 569
MhlI GDGCHC 2 cut(s) 41, 280
MluCI AATT 2 cut(s) 362, 496
MlyI GAGTC 3 cut(s) 130, 206, 421
MnlI CCTC 9 cut(s) 4, 45, 94, 97, 105, 220, 268, 343, 537
MseI TTAA 2 cut(s) 606, 623
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 3 cut(s) 146, 267, 286
MvaI CCWGG 2 cut(s) 267, 286
NciI CCSGG 1 cut(s) 146
NdeII GATC 3 cut(s) 262, 583, 640
NlaIII CATG 3 cut(s) 350, 537, 553
NlaIV GGNNCC 2 cut(s) 246, 278
NmeAIII GCCGAG 1 cut(s) 60
PfeI GAWTC 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 291
PkrI GCNGC 2 cut(s) 49, 355
PleI GAGTC 3 cut(s) 129, 205, 421
PpsI GAGTC 3 cut(s) 129, 205, 421
Psp6I CCWGG 2 cut(s) 265, 284
PspGI CCWGG 2 cut(s) 265, 284
PspN4I GGNNCC 2 cut(s) 246, 278
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 2 cut(s) 276, 277
PstNI CAGNNNCTG 1 cut(s) 291
RsaI GTAC 2 cut(s) 95, 218
RsaNI GTAC 2 cut(s) 94, 217
RseI CAYNNNNRTG 1 cut(s) 380
SaqAI TTAA 2 cut(s) 606, 623
SatI GCNGC 2 cut(s) 48, 354
Sau3AI GATC 3 cut(s) 262, 583, 640
Sau96I GGNCC 2 cut(s) 276, 277
SchI GAGTC 3 cut(s) 130, 206, 421
ScrFI CCNGG 3 cut(s) 146, 267, 286
SduI GDGCHC 2 cut(s) 41, 280
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 2 cut(s) 362, 496
SsiI CCGC 2 cut(s) 5, 47
StyD4I CCNGG 3 cut(s) 144, 265, 284
TaaI ACNGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 84, 222, 639
TasI AATT 2 cut(s) 362, 496
TatI WGTACW 2 cut(s) 93, 216
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 2 cut(s) 606, 623
Tru9I TTAA 2 cut(s) 606, 623
TscAI CASTG 4 cut(s) 72, 255, 508, 535
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 155
TspRI CASTG 4 cut(s) 72, 255, 508, 535
Van91I CCANNNNNTGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.