FvH4_3g21280

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0021044
Physical Location & Seq
Reverse (-)
14262482 .. 14265989
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21280.t1

Sequence Viewer

Length: 1146 bp
ATGGATTACATGTGCAGAAACTTAGAGAAAAATGAGCTCACAGGCTCGGTTCCTGCCGGGCTTATTGCAAAAAGAAATGATGGTTTGTCATTGAGTTTGTGTGGAAATCCAAGTTTAACGGGGCAGGTTCCTTGCGAGAAGAAGAGGAACCAGTTTGTTATTCCGGTCGTTGTGTCAATTATTGTCATGTTATTGGTCTCATCCATTGCAGCAGCTATATTGTGGAACTCTAAGAGGTTTCGTGCTCATTTGAAGTACTGGAGAGTTGGTGAAGCAGGTGCAATTACTGAATCTGAGATACATAATAGAAAAGGACACGATGTGTTTTTGAGTTTTAGAGGTGAGGATACACGCACAGGTTTTGCACGTTATCTATACGAAGCTTTAGAGAAAGAAGGAATCAAGACATTTTTTGATGATAAAAAGCTCAAGAAAGGAGATGAAATAGCAGTTACACTAGTCGAAGCAATAGAAGCATCAGAGATTTCTATCATTCTATTCTCAGAAAACTATGTTTCGTCAAGTTGGTGTTTGGCTGAACTTCTACAAATCCTTGAATGTAGAAAGACAAAAGATCAACGAGTCATCCCAGTTTTTTACAAAGTAGATCCATCACACGTACGACATCAAACTGATAGTTTTTCCCTCAACAAGTGGATAGACAAAAGGCATGTCAATGTTCCCGTATGGGAGAAAGCTCTTACAGAAGTAGCGAGTTTGGTGGGATTTCATTACAAGGACGGGTATGAATTTGAAACGATCAACAAGATTGTTAAAGAAACATCAGATGCCAAGGAGCAAAAGAAGGAAAAATCACTTGAAAGGTTCGTCTCAATACGCCCACCAGGTGGAAATGACGAAATAGTCATCTACATGACTTCATCAGCTAAGGACAAGAATTTCCAGAATTGCCAGTTAATGCTGCAGAGCTTAAGCCGATTTGACCCGCTGAAGCTTTGGCAGCAGCGTGACGTTTCAGAGAAAAATTTCAGACAAGAGTTGAAAATGATGATGAAAAAAGAGCTGCCTCCTCCAAGAATGTTTTGTGAAAGGAAAATATGTGGGTGGGACGTGGTGCAACAAGCCGGGGCAATCATCAGCGTCTCAGAAACAGAGATCAATGGACGTGGTGGTTCACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

382

Amino Acids

43.51

Weight (kDa)

8.73

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 106 - 271 9.1e-46 TIR domain
TIR_2 PF13676 108 - 223 3.9e-20 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21280
rosa_laevigata RLG00000033693 RLG00000033696
rosa_multiflora Rmu_sc0000712.1_g000004
rosa_rugosa Rorug05G0156600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
AasI GACNNNNNNGTC 1 cut(s) 863
Acc36I ACCTGC 2 cut(s) 115, 266
AccB7I CCANNNNNTGG 1 cut(s) 848
AciI CCGC 1 cut(s) 947
AclWI GGATC 1 cut(s) 602
AcsI RAATTY 3 cut(s) 749, 898, 985
AcuI CTGAAG 1 cut(s) 971
AdeI CACNNNGTG 2 cut(s) 322, 848
AfaI GTAC 2 cut(s) 257, 621
AfiI CCNNNNNNNGG 1 cut(s) 848
AflII CTTAAG 1 cut(s) 931
AflIII ACRYGT 2 cut(s) 9, 616
AgsI TTSAA 5 cut(s) 253, 557, 755, 821, 1003
AhlI ACTAGT 1 cut(s) 457
AjiI CACGTC 2 cut(s) 1072, 1127
AjnI CCWGG 1 cut(s) 844
AluBI AGCT 9 cut(s) 37, 215, 383, 427, 698, 887, 930, 955, 1024
AluI AGCT 9 cut(s) 37, 215, 383, 427, 698, 887, 930, 955, 1024
Alw21I GWGCWC 2 cut(s) 39, 247
Alw26I GTCTC 3 cut(s) 202, 835, 1108
AlwI GGATC 1 cut(s) 602
ApeKI GCWGC 6 cut(s) 209, 212, 922, 961, 964, 1024
ApoI RAATTY 3 cut(s) 749, 898, 985
ArsI GACNNNNNNTTYG 2 cut(s) 657, 689
Asp700I GAANNNNTTC 1 cut(s) 986
AsuC2I CCSGG 2 cut(s) 58, 1087
AsuHPI GGTGA 3 cut(s) 281, 353, 1128
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 39, 247
BbvI GCAGC 6 cut(s) 221, 224, 909, 973, 976, 1011
BccI CCATC 2 cut(s) 74, 619
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 1 cut(s) 340
BcnI CCSGG 2 cut(s) 58, 1087
BcoDI GTCTC 3 cut(s) 202, 835, 1108
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 923
BfrI CTTAAG 1 cut(s) 931
BfuAI ACCTGC 2 cut(s) 115, 266
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 6 cut(s) 210, 213, 923, 962, 965, 1025
BlsI GCNGC 6 cut(s) 211, 214, 924, 963, 966, 1026
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 3 cut(s) 58, 846, 1087
BmgBI CACGTC 2 cut(s) 1072, 1127
BmiI GGNNCC 3 cut(s) 51, 129, 149
BmrFI CCNGG 3 cut(s) 58, 846, 1087
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 2 cut(s) 485, 778
BmuI ACTGGG 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 2 cut(s) 58, 1087
BsaAI YACGTR 1 cut(s) 619
BsaBI GATNNNNATC 1 cut(s) 488
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 792, 1086
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 5 cut(s) 151, 263, 590, 913, 1139
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 488
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 792, 1086
BseGI GGATG 2 cut(s) 200, 585
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 3 cut(s) 285, 516, 1119
BseNI ACTGG 5 cut(s) 151, 263, 590, 913, 1139
BseRI GAGGAG 1 cut(s) 1020
BseXI GCAGC 6 cut(s) 221, 224, 909, 973, 976, 1011
BsgI GTGCAG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 39, 247
BsiSI CCGG 3 cut(s) 57, 164, 1086
BsiWI CGTACG 1 cut(s) 619
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 3 cut(s) 202, 835, 1108
BsmBI CGTCTC 2 cut(s) 835, 1108
BsmFI GGGAC 1 cut(s) 1082
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 39, 247
Bsp143I GATC 4 cut(s) 574, 607, 759, 1116
BspACI CCGC 1 cut(s) 947
BspCNI CTCAG 3 cut(s) 286, 515, 1118
BspLI GGNNCC 3 cut(s) 51, 129, 149
BspMAI CTGCAG 1 cut(s) 927
BspMI ACCTGC 2 cut(s) 115, 266
BspPI GGATC 1 cut(s) 602
BspTI CTTAAG 1 cut(s) 931
BspTNI GGTCTC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 5 cut(s) 151, 263, 590, 913, 1139
BssECI CCNNGG 2 cut(s) 792, 1086
BssMI GATC 4 cut(s) 574, 607, 759, 1116
BssT1I CCWWGG 1 cut(s) 792
Bst2UI CCWGG 1 cut(s) 846
Bst6I CTCTTC 1 cut(s) 137
BstAFI CTTAAG 1 cut(s) 931
BstBAI YACGTR 1 cut(s) 619
BstDEI CTNAG 6 cut(s) 22, 231, 294, 502, 888, 1105
BstF5I GGATG 2 cut(s) 200, 585
BstKTI GATC 4 cut(s) 577, 610, 762, 1119
BstMAI GTCTC 3 cut(s) 202, 835, 1108
BstMBI GATC 4 cut(s) 574, 607, 759, 1116
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 473, 961
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 2 cut(s) 13, 674
BstSCI CCNGG 3 cut(s) 56, 844, 1085
BstSFI CTRYAG 1 cut(s) 923
BstV1I GCAGC 6 cut(s) 221, 224, 909, 973, 976, 1011
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BsuI GTATCC 1 cut(s) 340
BtrI CACGTC 2 cut(s) 1072, 1127
BtsCI GGATG 2 cut(s) 200, 585
BveI ACCTGC 2 cut(s) 115, 266
CseI GACGC 1 cut(s) 1090
CsiI ACCWGGT 1 cut(s) 844
Csp6I GTAC 2 cut(s) 256, 620
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CviAII CATG 4 cut(s) 10, 187, 671, 874
CviQI GTAC 2 cut(s) 256, 620
DdeI CTNAG 6 cut(s) 22, 231, 294, 502, 888, 1105
DpnI GATC 4 cut(s) 576, 609, 761, 1118
DpnII GATC 4 cut(s) 574, 607, 759, 1116
DraIII CACNNNGTG 2 cut(s) 322, 848
DrdI GACNNNNNNGTC 1 cut(s) 863
DseDI GACNNNNNNGTC 1 cut(s) 863
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 971
EcoICRI GAGCTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 792
Esp3I CGTCTC 2 cut(s) 835, 1108
FaeI CATG 4 cut(s) 13, 190, 674, 877
FaqI GGGAC 1 cut(s) 1082
FatI CATG 4 cut(s) 9, 186, 670, 873
FauI CCCGC 1 cut(s) 954
Fnu4HI GCNGC 6 cut(s) 210, 213, 923, 962, 965, 1025
FokI GGATG 2 cut(s) 187, 572
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 6 cut(s) 210, 213, 923, 962, 965, 1025
FspBI CTAG 1 cut(s) 458
GluI GCNGC 6 cut(s) 210, 213, 923, 962, 965, 1025
GsuI CTGGAG 1 cut(s) 280
HapII CCGG 3 cut(s) 57, 164, 1086
HgaI GACGC 1 cut(s) 1090
Hin1II CATG 4 cut(s) 13, 190, 674, 877
HindIII AAGCTT 2 cut(s) 381, 953
HinfI GANTC 3 cut(s) 290, 399, 582
HpaII CCGG 3 cut(s) 57, 164, 1086
HphI GGTGA 3 cut(s) 281, 353, 1128
Hpy166II GTNNAC 1 cut(s) 1136
Hpy188I TCNGA 7 cut(s) 295, 481, 505, 787, 979, 992, 1108
Hpy188III TCNNGA 3 cut(s) 403, 430, 904
Hpy8I GTNNAC 1 cut(s) 1136
HpyAV CCTTC 2 cut(s) 389, 799
HpyCH4IV ACGT 5 cut(s) 367, 618, 972, 1071, 1126
HpyCH4V TGCA 7 cut(s) 15, 68, 209, 281, 365, 925, 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 961
HpyF3I CTNAG 6 cut(s) 22, 231, 294, 502, 888, 1105
HpySE526I ACGT 5 cut(s) 367, 618, 972, 1071, 1126
Hsp92II CATG 4 cut(s) 13, 190, 674, 877
Kzo9I GATC 4 cut(s) 574, 607, 759, 1116
LmnI GCTCC 1 cut(s) 796
Lsp1109I GCAGC 6 cut(s) 221, 224, 909, 973, 976, 1011
LweI GCATC 2 cut(s) 485, 778
MabI ACCWGGT 1 cut(s) 844
MaeI CTAG 1 cut(s) 458
MaeII ACGT 5 cut(s) 367, 618, 972, 1071, 1126
MaeIII GTNAC 2 cut(s) 451, 968
MalI GATC 4 cut(s) 576, 609, 761, 1118
MboI GATC 4 cut(s) 574, 607, 759, 1116
MboII GAAGA 2 cut(s) 151, 154
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 2 cut(s) 39, 247
MluCI AATT 6 cut(s) 177, 282, 749, 898, 907, 985
MlyI GAGTC 1 cut(s) 591
MnlI CCTC 7 cut(s) 138, 228, 332, 337, 656, 1038, 1041
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 4 cut(s) 116, 774, 917, 932
MslI CAYNNNNRTG 2 cut(s) 675, 872
MspA1I CMGCKG 1 cut(s) 949
MspCI CTTAAG 1 cut(s) 931
MspI CCGG 3 cut(s) 57, 164, 1086
MspR9I CCNGG 3 cut(s) 58, 846, 1087
MvaI CCWGG 1 cut(s) 846
MwoI GCNNNNNNNGC 2 cut(s) 473, 961
NciI CCSGG 2 cut(s) 58, 1087
NdeII GATC 4 cut(s) 574, 607, 759, 1116
NlaIII CATG 4 cut(s) 13, 190, 674, 877
NlaIV GGNNCC 3 cut(s) 51, 129, 149
NmuCI GTSAC 1 cut(s) 968
NspI RCATGY 2 cut(s) 13, 674
PaqCI CACCTGC 1 cut(s) 266
PciI ACATGT 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 2 cut(s) 290, 399
Pfl23II CGTACG 1 cut(s) 619
PflMI CCANNNNNTGG 1 cut(s) 848
PkrI GCNGC 6 cut(s) 211, 214, 924, 963, 966, 1026
PleI GAGTC 1 cut(s) 590
PpsI GAGTC 1 cut(s) 590
Ppu21I YACGTR 1 cut(s) 619
PscI ACATGT 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspLI CGTACG 1 cut(s) 619
PspN4I GGNNCC 3 cut(s) 51, 129, 149
PstI CTGCAG 1 cut(s) 927
PsuI RGATCY 1 cut(s) 607
RsaI GTAC 2 cut(s) 257, 621
RsaNI GTAC 2 cut(s) 256, 620
RseI CAYNNNNRTG 2 cut(s) 675, 872
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 116, 774, 917, 932
SatI GCNGC 6 cut(s) 210, 213, 923, 962, 965, 1025
Sau3AI GATC 4 cut(s) 574, 607, 759, 1116
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 1 cut(s) 591
ScrFI CCNGG 3 cut(s) 58, 846, 1087
SduI GDGCHC 2 cut(s) 39, 247
SexAI ACCWGGT 1 cut(s) 844
SfaNI GCATC 2 cut(s) 485, 778
SfcI CTRYAG 1 cut(s) 923
SmiMI CAYNNNNRTG 2 cut(s) 675, 872
SmlI CTYRAG 2 cut(s) 428, 931
SmoI CTYRAG 2 cut(s) 428, 931
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 6 cut(s) 177, 282, 749, 898, 907, 985
SsiI CCGC 1 cut(s) 947
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 56, 844, 1085
StyI CCWWGG 1 cut(s) 792
TaiI ACGT 5 cut(s) 370, 621, 975, 1074, 1129
TaqI TCGA 1 cut(s) 462
TasI AATT 6 cut(s) 177, 282, 749, 898, 907, 985
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 2 cut(s) 290, 399
Tru1I TTAA 4 cut(s) 116, 774, 917, 932
Tru9I TTAA 4 cut(s) 116, 774, 917, 932
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 6 cut(s) 209, 212, 922, 961, 964, 1024
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 5 cut(s) 456, 719, 762, 870, 1028
Van91I CCANNNNNTGG 1 cut(s) 848
Vha464I CTTAAG 1 cut(s) 931
XapI RAATTY 3 cut(s) 749, 898, 985
XceI RCATGY 2 cut(s) 13, 674
XmnI GAANNNNTTC 1 cut(s) 986
XspI CTAG 1 cut(s) 458
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.