RLG00000033693

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0021044
Physical Location & Seq
Reverse (-)
30950995 .. 30952382
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033693

Sequence Viewer

Length: 867 bp
ATGTGCAGAAACTTGGAGAAAAATGAGCTCACAGGTTCAGTTCCTGCCGGGCTTATAGCAAAAACAAAGGATGGTTTGTCGTTGAGTTTGTGCGAAAATCCAAGTTTATCAGGACAGGTTCCTTGCAAGAAGAAGAAGAACAAGTTTGCTATTTCGGTCGTTGTGTCAATTATTGTAATGTTATTGGTCTCATCCATTGCGGCAGCTATATTGTGGAAAGTAAAAAAGGGTCATGCTGTGTGGAAGTATTGGAGAGTTGAAGTGGGACATGATGTGTTTTTGAGTTTTAGAGGTGAGGATACACGCAAAGGTTTTGCGCGTAATCTGTACGAAGCTTTAGAGGAAAAAGGAATCAAGACATTTTTTGATGATGAAACGCTCAAGCCGGGAGATGAAATAGCACCTGCACTTCTCGATGCAATAGAAGCATCAAAGATTTCGGTCATTATAATCTCAGAAAACTATGCTTCGTCAAGTTGGTGTTTGGGGGAACTTGTTAAGATCCTTGATTGTAAAAAGTCAAAGCATCAACGTGTTATCCCAGTTTTTTACAATGTGGATCCATCACACGTACGACATCAATCTGGTAGTTTTTCCCTTCAGAAGTGGATAGAGAAAAAGGAGCTTCCCGCATGGCAGAAAGCTCTTACCGAAGTAGCAAGTTTGGCGGGAATTCATAAAACGAACGAGTATGAATCTAAAACGATTGGCGACATTGTTAAACAAATATCAGATGTCAACGAGCAAAAGAAGAAAGAAGCTGGAAACTCAGTTGATGGGTTCGACAAAATATCCCCACAAGCGGTGGCCGGGTCAGTCAGCGTCTCAGAAAAAAAGAGCGATGGATCTGGTGGTTCACCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

289

Amino Acids

31.57

Weight (kDa)

8.89

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 90 - 253 6.4e-52 TIR domain
TIR_2 PF13676 92 - 189 6.9e-21 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21280
rosa_laevigata RLG00000033693 RLG00000033696
rosa_multiflora Rmu_sc0000712.1_g000004
rosa_rugosa Rorug05G0156600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
AccII CGCG 1 cut(s) 319
AciI CCGC 4 cut(s) 200, 630, 668, 803
AclWI GGATC 4 cut(s) 496, 554, 567, 853
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 1 cut(s) 672
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 2 cut(s) 329, 573
AfiI CCNNNNNNNGG 1 cut(s) 802
AflIII ACRYGT 2 cut(s) 532, 568
AgsI TTSAA 1 cut(s) 260
AluBI AGCT 6 cut(s) 28, 206, 335, 625, 644, 761
AluI AGCT 6 cut(s) 28, 206, 335, 625, 644, 761
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 193, 829
AlwI GGATC 4 cut(s) 496, 554, 567, 853
AlwNI CAGNNNCTG 1 cut(s) 44
AoxI GGCC 1 cut(s) 807
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 1 cut(s) 672
AspLEI GCGC 1 cut(s) 319
AsuC2I CCSGG 3 cut(s) 49, 387, 811
AsuHPI GGTGA 2 cut(s) 305, 849
BamHI GGATCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 215
BccI CCATC 4 cut(s) 65, 571, 770, 836
BciVI GTATCC 1 cut(s) 292
BcnI CCSGG 3 cut(s) 49, 387, 811
BcoDI GTCTC 2 cut(s) 193, 829
BfuAI ACCTGC 1 cut(s) 412
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 2 cut(s) 201, 204
BlsI GCNGC 2 cut(s) 202, 205
Bme1390I CCNGG 3 cut(s) 49, 387, 811
BmiI GGNNCC 2 cut(s) 120, 561
BmrFI CCNGG 3 cut(s) 49, 387, 811
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 3 cut(s) 406, 437, 535
BmuI ACTGGG 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 3 cut(s) 49, 387, 811
BsaAI YACGTR 1 cut(s) 571
BsaI GGTCTC 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse1I ACTGG 2 cut(s) 542, 860
Bse3DI GCAATG 1 cut(s) 195
BseGI GGATG 2 cut(s) 76, 191
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 3 cut(s) 468, 783, 840
BseNI ACTGG 2 cut(s) 542, 860
BseXI GCAGC 1 cut(s) 215
BsgI GTGCAG 2 cut(s) 25, 390
Bsh1236I CGCG 1 cut(s) 319
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 1 cut(s) 809
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 3 cut(s) 48, 386, 810
BsiWI CGTACG 1 cut(s) 571
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 2 cut(s) 193, 829
BsmBI CGTCTC 1 cut(s) 829
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 1 cut(s) 809
Bso31I GGTCTC 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 3 cut(s) 501, 559, 845
BspACI CCGC 4 cut(s) 200, 630, 668, 803
BspANI GGCC 1 cut(s) 809
BspCNI CTCAG 3 cut(s) 467, 782, 839
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 120, 561
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 4 cut(s) 496, 554, 567, 853
BspTNI GGTCTC 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 2 cut(s) 542, 860
BssMI GATC 3 cut(s) 501, 559, 845
BstBAI YACGTR 1 cut(s) 571
BstDEI CTNAG 3 cut(s) 454, 769, 826
BstF5I GGATG 2 cut(s) 76, 191
BstFNI CGCG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 3 cut(s) 504, 562, 848
BstMAI GTCTC 2 cut(s) 193, 829
BstMBI GATC 3 cut(s) 501, 559, 845
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 2 cut(s) 425, 665
BstSCI CCNGG 3 cut(s) 47, 385, 809
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 3 cut(s) 501, 559, 845
BstYI RGATCY 3 cut(s) 501, 559, 845
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 1 cut(s) 809
BtgZI GCGATG 1 cut(s) 855
BtsCI GGATG 2 cut(s) 76, 191
BveI ACCTGC 1 cut(s) 412
CaiI CAGNNNCTG 1 cut(s) 44
CfoI GCGC 1 cut(s) 319
CseI GACGC 1 cut(s) 811
Csp6I GTAC 2 cut(s) 328, 572
CviAII CATG 3 cut(s) 233, 269, 633
CviJI RGCY 9 cut(s) 28, 52, 206, 335, 385, 625, 644, 761, 809
CviKI_1 RGCY 9 cut(s) 28, 52, 206, 335, 385, 625, 644, 761, 809
CviQI GTAC 2 cut(s) 328, 572
DdeI CTNAG 3 cut(s) 454, 769, 826
DpnI GATC 3 cut(s) 503, 561, 847
DpnII GATC 3 cut(s) 501, 559, 845
EaeI YGGCCR 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 672
EcoT38I GRGCYC 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 829
FaeI CATG 3 cut(s) 236, 272, 636
FaiI YATR 9 cut(s) 56, 209, 234, 270, 449, 465, 634, 678, 693
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 232, 268, 632
FauI CCCGC 2 cut(s) 637, 661
Fnu4HI GCNGC 2 cut(s) 201, 204
FokI GGATG 2 cut(s) 83, 178
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 201, 204
GlaI GCGC 1 cut(s) 318
GluI GCNGC 2 cut(s) 201, 204
HaeIII GGCC 1 cut(s) 809
HapII CCGG 3 cut(s) 48, 386, 810
HgaI GACGC 1 cut(s) 811
HhaI GCGC 1 cut(s) 319
Hin1II CATG 3 cut(s) 236, 272, 636
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 2 cut(s) 351, 695
HpaII CCGG 3 cut(s) 48, 386, 810
HphI GGTGA 2 cut(s) 305, 849
Hpy166II GTNNAC 2 cut(s) 739, 857
Hpy188I TCNGA 4 cut(s) 457, 603, 733, 829
Hpy188III TCNNGA 3 cut(s) 111, 355, 413
Hpy8I GTNNAC 2 cut(s) 739, 857
HpyAV CCTTC 1 cut(s) 608
HpyCH4IV ACGT 2 cut(s) 532, 570
HpyCH4V TGCA 4 cut(s) 6, 126, 407, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 665
HpyF3I CTNAG 3 cut(s) 454, 769, 826
HpySE526I ACGT 2 cut(s) 532, 570
Hsp92II CATG 3 cut(s) 236, 272, 636
HspAI GCGC 1 cut(s) 317
Kzo9I GATC 3 cut(s) 501, 559, 845
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 3 cut(s) 406, 437, 535
MaeII ACGT 2 cut(s) 532, 570
MalI GATC 3 cut(s) 503, 561, 847
MboI GATC 3 cut(s) 501, 559, 845
MboII GAAGA 4 cut(s) 142, 145, 148, 763
MflI RGATCY 3 cut(s) 501, 559, 845
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 168, 672
MnlI CCTC 3 cut(s) 284, 289, 334
MseI TTAA 3 cut(s) 498, 720, 865
MslI CAYNNNNRTG 1 cut(s) 531
MspI CCGG 3 cut(s) 48, 386, 810
MspR9I CCNGG 3 cut(s) 49, 387, 811
MvnI CGCG 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 425, 665
NciI CCSGG 3 cut(s) 49, 387, 811
NdeII GATC 3 cut(s) 501, 559, 845
NlaIII CATG 3 cut(s) 236, 272, 636
NlaIV GGNNCC 2 cut(s) 120, 561
PaqCI CACCTGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 351, 695
Pfl23II CGTACG 1 cut(s) 571
PkrI GCNGC 2 cut(s) 202, 205
Ppu21I YACGTR 1 cut(s) 571
PsiI TTATAA 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 30
PspLI CGTACG 1 cut(s) 571
PspN4I GGNNCC 2 cut(s) 120, 561
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 3 cut(s) 501, 559, 845
RsaI GTAC 2 cut(s) 329, 573
RsaNI GTAC 2 cut(s) 328, 572
RseI CAYNNNNRTG 1 cut(s) 531
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 3 cut(s) 498, 720, 865
SatI GCNGC 2 cut(s) 201, 204
Sau3AI GATC 3 cut(s) 501, 559, 845
ScrFI CCNGG 3 cut(s) 49, 387, 811
SduI GDGCHC 1 cut(s) 30
SfaNI GCATC 3 cut(s) 406, 437, 535
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 2 cut(s) 168, 672
SsiI CCGC 4 cut(s) 200, 630, 668, 803
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 47, 385, 809
TaiI ACGT 2 cut(s) 535, 573
TaqI TCGA 2 cut(s) 414, 783
TaqII GACCGA 2 cut(s) 145, 430
TasI AATT 2 cut(s) 168, 672
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 2 cut(s) 351, 695
Tru1I TTAA 3 cut(s) 498, 720, 865
Tru9I TTAA 3 cut(s) 498, 720, 865
TseI GCWGC 1 cut(s) 203
TspDTI ATGAA 4 cut(s) 387, 408, 665, 708
XapI RAATTY 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.