Rorug05G0156600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0021044
Physical Location & Seq
Forward (+)
14603967 .. 14605035
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0156600.1

Sequence Viewer

Length: 990 bp
ATGTCCTCAGCCGAGTCAAACCGAGTTGCGTTTTTCGTGGACATGTTTTGTACATCCATGATCGACGTTGCCAACGAGCTTGAGATTCCTTGTTATATGTATTTCGCTTCTCCGGCGACGTTTCTGAGCTTCATGTTTCATATTCCGGTCTTGGACGCCGAAATTTCAACCGACTTTGGCGACTTGGATACTGAGTTGACTATACCTGGTTTTGCTAACTCGGTGCCTCCGCTGGTGTTGCCTACGGCGCTGTTGAGTAAGAAGGGGGATACGTACCCTAGCTATTTATCCCATACGGCAAGGTACAAGGAACCCAAGGGTATTGTTGTCAATACGTTCAAGGAATTGGAGCCACACGCGCTTAGCTCGCTGGCTGCGAGTGATGTGCCACGAGTTCACGCTATTGGGCCGATTCTTGATCTTAATGGGCCGGGCCAATGGCAGGATCCCGACCGGTACGAGAGCGTGGTGAAGTGGCTTGATGACCAGCCTCCATCGTCTGTGGTGCTGTTGTGTTTTGGGAGCATGGGGAGTCTTAGTGGGCCTCAGGTGAGGGAGATAGCATTTGGGCTGGAAAGGGCTGGGTTTCGGTTCTTATGGGCATTGCGTGAACCGCCCAAGTCCGCTATGTACCTCCCAAGCGACCCCGCAAACGTAGACGAGGTATTGCCACACGGGTTTTTAGAACGGACATGCAAATTGGGCTTAGTGTGCGGGTGGGTTCCGAGGGTTCGTATCTCATTGCGGGTGGAGCTCTATTTTGGAGAGCTTGTGGTATGGGTGGAGATTAGGCTGGACTATAGGGAGGGAAGTGATTTGGTGTCGGCGGAGGAGGTGGAGAGAAGCATAAAGAGCTTGATGGACGGTGATCATGTGGTGAGGACTAAGGTGAAAGACATGAGAGAGAAGAGTAGGGGAGCATTGATGGAAAATGGGTCTGCATACAAAGCACTAGAAGGTTTAATTGAGGAGCTAATGCCCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

329

Amino Acids

36.67

Weight (kDa)

4.95

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21280
rosa_laevigata RLG00000033693 RLG00000033696
rosa_multiflora Rmu_sc0000712.1_g000004
rosa_rugosa Rorug05G0156600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 359
AciI CCGC 7 cut(s) 230, 614, 624, 648, 714, 745, 827
AclWI GGATC 2 cut(s) 440, 453
AcsI RAATTY 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 5 cut(s) 52, 275, 305, 458, 632
AfiI CCNNNNNNNGG 1 cut(s) 442
AflIII ACRYGT 1 cut(s) 42
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 168, 340
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 8 cut(s) 79, 129, 282, 366, 754, 769, 855, 973
AluI AGCT 8 cut(s) 79, 129, 282, 366, 754, 769, 855, 973
Alw21I GWGCWC 1 cut(s) 756
AlwI GGATC 2 cut(s) 440, 453
AoxI GGCC 4 cut(s) 407, 428, 433, 542
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 162
AsiGI ACCGGT 1 cut(s) 453
AspLEI GCGC 2 cut(s) 250, 361
AspS9I GGNCC 4 cut(s) 407, 428, 433, 542
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 5 cut(s) 481, 562, 878, 889, 901
AxyI CCTNAGG 1 cut(s) 546
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 756
BauI CACGAG 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 756
BbvCI CCTCAGC 1 cut(s) 7
BbvI GCAGC 1 cut(s) 361
BccI CCATC 3 cut(s) 502, 853, 919
BceAI ACGGC 2 cut(s) 261, 312
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 2 cut(s) 181, 262
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 1 cut(s) 432
BfaI CTAG 2 cut(s) 279, 953
BfmI CTRYAG 1 cut(s) 799
BfoI RGCGCY 1 cut(s) 251
BfuI GTATCC 2 cut(s) 181, 262
BisI GCNGC 1 cut(s) 375
BlpI GCTNAGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 207, 432
BmgT120I GGNCC 4 cut(s) 407, 428, 433, 542
BmiI GGNNCC 5 cut(s) 225, 312, 351, 447, 723
BmrFI CCNGG 2 cut(s) 207, 432
Bpu10I CCTNAGC 1 cut(s) 7
Bpu1102I GCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 315, 725
BsaWI WCCGGW 2 cut(s) 145, 453
BsaXI ACNNNNNCTCC 4 cut(s) 523, 553, 756, 786
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 453
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 2 cut(s) 602, 740
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 315, 725
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 2 cut(s) 602, 740
BseMII CTCAG 4 cut(s) 21, 116, 183, 560
BseRI GAGGAG 2 cut(s) 845, 983
BseXI GCAGC 1 cut(s) 361
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 4 cut(s) 409, 430, 435, 544
BshNI GGYRCC 1 cut(s) 223
BshTI ACCGGT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 4 cut(s) 113, 146, 431, 454
BslI CCNNNNNNNGG 1 cut(s) 442
BsnI GGCC 4 cut(s) 409, 430, 435, 544
Bsp1286I GDGCHC 1 cut(s) 756
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 4 cut(s) 60, 418, 445, 868
Bsp1720I GCTNAGC 1 cut(s) 362
BspACI CCGC 7 cut(s) 230, 614, 624, 648, 714, 745, 827
BspANI GGCC 4 cut(s) 409, 430, 435, 544
BspCNI CTCAG 4 cut(s) 20, 117, 184, 559
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 5 cut(s) 225, 312, 351, 447, 723
BspPI GGATC 2 cut(s) 440, 453
BspT107I GGYRCC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 602, 740
BsrFI RCCGGY 1 cut(s) 453
BsrGI TGTACA 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 2 cut(s) 315, 725
BssMI GATC 4 cut(s) 60, 418, 445, 868
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 866
Bst6I CTCTTC 1 cut(s) 902
BstACI GRCGYC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 273
BstC8I GCNNGC 2 cut(s) 368, 372
BstDEI CTNAG 8 cut(s) 7, 125, 192, 362, 536, 546, 706, 885
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 250, 361
BstKTI GATC 4 cut(s) 63, 421, 448, 871
BstMBI GATC 4 cut(s) 60, 418, 445, 868
BstMCI CGRYCG 1 cut(s) 454
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 2 cut(s) 46, 696
BstSCI CCNGG 2 cut(s) 205, 430
BstSFI CTRYAG 1 cut(s) 799
BstSNI TACGTA 1 cut(s) 273
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 2 cut(s) 181, 262
BsuRI GGCC 4 cut(s) 409, 430, 435, 544
BtsCI GGATG 1 cut(s) 53
Cac8I GCNNGC 2 cut(s) 368, 372
CfoI GCGC 2 cut(s) 250, 361
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 4 cut(s) 407, 428, 433, 542
CseI GACGC 1 cut(s) 164
CsiI ACCWGGT 1 cut(s) 205
Csp6I GTAC 5 cut(s) 51, 274, 304, 457, 631
CspAI ACCGGT 1 cut(s) 453
CviAII CATG 7 cut(s) 43, 58, 133, 526, 693, 872, 898
CviQI GTAC 5 cut(s) 51, 274, 304, 457, 631
DdeI CTNAG 8 cut(s) 7, 125, 192, 362, 536, 546, 706, 885
DpnI GATC 4 cut(s) 62, 420, 447, 870
DpnII GATC 4 cut(s) 60, 418, 445, 868
Eam1104I CTCTTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 902
EciI GGCGGA 1 cut(s) 842
Ecl136II GAGCTC 1 cut(s) 754
Eco105I TACGTA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 756
Eco53kI GAGCTC 1 cut(s) 754
Eco81I CCTNAGG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 754
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 7 cut(s) 46, 61, 136, 529, 696, 875, 901
FatI CATG 7 cut(s) 42, 57, 132, 525, 692, 871, 897
FauI CCCGC 3 cut(s) 655, 707, 738
FbaI TGATCA 1 cut(s) 868
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 2 cut(s) 279, 953
GlaI GCGC 2 cut(s) 249, 360
GluI GCNGC 1 cut(s) 375
GsaI CCCAGC 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 4 cut(s) 409, 430, 435, 544
HapII CCGG 4 cut(s) 113, 146, 431, 454
HgaI GACGC 1 cut(s) 164
HhaI GCGC 2 cut(s) 250, 361
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 7 cut(s) 46, 61, 136, 529, 696, 875, 901
Hin6I GCGC 2 cut(s) 248, 359
HinP1I GCGC 2 cut(s) 248, 359
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 4 cut(s) 14, 85, 412, 532
HpaII CCGG 4 cut(s) 113, 146, 431, 454
HphI GGTGA 5 cut(s) 481, 562, 878, 889, 901
Hpy166II GTNNAC 5 cut(s) 40, 198, 397, 611, 658
Hpy188I TCNGA 2 cut(s) 126, 726
Hpy188III TCNNGA 2 cut(s) 416, 449
Hpy8I GTNNAC 5 cut(s) 40, 198, 397, 611, 658
Hpy99I CGWCG 2 cut(s) 68, 121
HpyAV CCTTC 2 cut(s) 256, 950
HpyCH4III ACNGT 1 cut(s) 866
HpyCH4IV ACGT 5 cut(s) 66, 119, 272, 335, 654
HpyCH4V TGCA 2 cut(s) 696, 941
HpyF3I CTNAG 8 cut(s) 7, 125, 192, 362, 536, 546, 706, 885
HpySE526I ACGT 5 cut(s) 66, 119, 272, 335, 654
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 7 cut(s) 46, 61, 136, 529, 696, 875, 901
HspAI GCGC 2 cut(s) 248, 359
Ksp22I TGATCA 1 cut(s) 868
Kzo9I GATC 4 cut(s) 60, 418, 445, 868
LmnI GCTCC 5 cut(s) 349, 522, 751, 917, 970
Lsp1109I GCAGC 1 cut(s) 361
MabI ACCWGGT 1 cut(s) 205
MaeI CTAG 2 cut(s) 279, 953
MaeII ACGT 5 cut(s) 66, 119, 272, 335, 654
MalI GATC 4 cut(s) 62, 420, 447, 870
MboI GATC 4 cut(s) 60, 418, 445, 868
MboII GAAGA 1 cut(s) 919
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 4 cut(s) 162, 344, 698, 963
MlyI GAGTC 2 cut(s) 23, 541
MseI TTAA 2 cut(s) 423, 962
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 4 cut(s) 113, 146, 431, 454
MspR9I CCNGG 2 cut(s) 207, 432
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 359
NciI CCSGG 1 cut(s) 432
NdeII GATC 4 cut(s) 60, 418, 445, 868
NlaIII CATG 7 cut(s) 46, 61, 136, 529, 696, 875, 901
NlaIV GGNNCC 5 cut(s) 225, 312, 351, 447, 723
NmeAIII GCCGAG 1 cut(s) 37
NspI RCATGY 2 cut(s) 46, 696
PciI ACATGT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 374
PfeI GAWTC 2 cut(s) 85, 412
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 2 cut(s) 22, 540
PpsI GAGTC 2 cut(s) 22, 540
Ppu21I YACGTR 1 cut(s) 273
PscI ACATGT 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 756
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 5 cut(s) 225, 312, 351, 447, 723
PspPI GGNCC 4 cut(s) 407, 428, 433, 542
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 5 cut(s) 52, 275, 305, 458, 632
RsaNI GTAC 5 cut(s) 51, 274, 304, 457, 631
SacI GAGCTC 1 cut(s) 756
SaqAI TTAA 2 cut(s) 423, 962
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 4 cut(s) 60, 418, 445, 868
Sau96I GGNCC 4 cut(s) 407, 428, 433, 542
SchI GAGTC 2 cut(s) 23, 541
ScrFI CCNGG 2 cut(s) 207, 432
SduI GDGCHC 1 cut(s) 756
SexAI ACCWGGT 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 799
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SnaBI TACGTA 1 cut(s) 273
Sse9I AATT 4 cut(s) 162, 344, 698, 963
SsiI CCGC 7 cut(s) 230, 614, 624, 648, 714, 745, 827
SspMI CTAG 2 cut(s) 279, 953
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 205, 430
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 866
TaiI ACGT 5 cut(s) 69, 122, 275, 338, 657
TaqI TCGA 1 cut(s) 63
TasI AATT 4 cut(s) 162, 344, 698, 963
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 2 cut(s) 85, 412
Tru1I TTAA 2 cut(s) 423, 962
Tru9I TTAA 2 cut(s) 423, 962
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 2 cut(s) 121, 128
TspGWI ACGGA 1 cut(s) 703
XapI RAATTY 1 cut(s) 162
XceI RCATGY 2 cut(s) 46, 696
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 2 cut(s) 279, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.