RLG00000033696

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0021044
Physical Location & Seq
Reverse (-)
31026223 .. 31028898
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033696

Sequence Viewer

Length: 726 bp
ATGTGCAGAAACTTGGAGAAAAATGAGCTCACAGGTTCAGTTCCTGCCGGGCTTATAGCAAAAGGAAAGGATGGTTTGTCATTGAGTTTGTGCGGAAATCCAAGTTTATCAAAGCAGGTTCCTTGCGAGAAGAAGAAGAAGAACGAGTTTGCTGTTCCGGTCGTTGTGTCAATTATTGTAATGTTGTTGGTCTCATCCATTGCAGCAGCTATATTGTGGAGATTAAAAAAGGATCATGCTGTTTGGAAGTACTGGAGAGTTGATAAGGGACATGATGTGTTTTTGAGTTTTAGAGGTGAGGATACACGCAAAGGCTTTGCACGTAGTCTGTACGAAGCTTTAGAGGAAAAAGGAATCAAGACATTTTTTGATGATGAAACGCAGAAGGCGGGAGATGAAATAGCACTTGCACTTCCCGATGCAATAGAAGCATCAAAGATTTCGGCAACAATCCGCCGCCACCACCATCTTGGCCACGTCGACGGAATAGATCAGCCGCCTGCCTTAAGTTCACCCCATTCAGCCACCGCAAATCTGGATGGTGTCGACGAACCCGTCACCCACGTCCCACATCAAACCAATCTGTTATGCCGCCAAGCAAGCGCCCAGCTCAGGGCGCACTCGTCGCGGGACATGGAAATCAATTTGAGAAACGAACTGACAGTTGCTGCAGAGATCGATTCAAGACCCTCGATCGGTCCAAAACTCGACGGAAAAACCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

242

Amino Acids

26.45

Weight (kDa)

8.31

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 91 - 149 4.4e-13 TIR domain
TIR_2 PF13676 93 - 147 2.2e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021044)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21280
rosa_laevigata RLG00000033693 RLG00000033696
rosa_multiflora Rmu_sc0000712.1_g000004
rosa_rugosa Rorug05G0156600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 106
AccI GTMKAC 2 cut(s) 480, 546
AccII CGCG 1 cut(s) 628
AciI CCGC 8 cut(s) 93, 389, 454, 457, 497, 528, 592, 628
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 472
AfaI GTAC 2 cut(s) 251, 332
AfiI CCNNNNNNNGG 3 cut(s) 612, 613, 695
AflII CTTAAG 1 cut(s) 505
AgsI TTSAA 1 cut(s) 684
AjiI CACGTC 2 cut(s) 478, 565
AluBI AGCT 4 cut(s) 28, 209, 338, 610
AluI AGCT 4 cut(s) 28, 209, 338, 610
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 240
AlwNI CAGNNNCTG 2 cut(s) 44, 668
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 3 cut(s) 203, 206, 668
AspLEI GCGC 2 cut(s) 605, 619
AspS9I GGNCC 1 cut(s) 698
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 3 cut(s) 308, 504, 550
AvaII GGWCC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 474
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 215, 218, 655
BccI CCATC 3 cut(s) 65, 474, 533
BciVI GTATCC 1 cut(s) 295
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 196
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 606
BfrI CTTAAG 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 106
BfuI GTATCC 1 cut(s) 295
BisI GCNGC 6 cut(s) 204, 207, 457, 497, 592, 669
BlsI GCNGC 6 cut(s) 205, 208, 458, 498, 593, 670
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 49
Bme18I GGWCC 1 cut(s) 698
BmgBI CACGTC 2 cut(s) 478, 565
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 49
BmsI GCATC 2 cut(s) 409, 440
BpmI CTGGAG 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 49
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 3 cut(s) 612, 613, 695
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 678
BseGI GGATG 3 cut(s) 76, 194, 544
BseLI CCNNNNNNNGG 3 cut(s) 612, 613, 695
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 625
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 3 cut(s) 215, 218, 655
BseYI CCCAGC 1 cut(s) 606
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 628
Bsh1285I CGRYCG 2 cut(s) 162, 696
BshFI GGCC 1 cut(s) 474
BshVI ATCGAT 1 cut(s) 678
BsiEI CGRYCG 2 cut(s) 162, 696
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 48, 158
BslFI GGGAC 3 cut(s) 282, 551, 644
BslI CCNNNNNNNGG 3 cut(s) 612, 613, 695
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 282, 551, 644
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 4 cut(s) 232, 490, 675, 693
BspACI CCGC 8 cut(s) 93, 389, 454, 457, 497, 528, 592, 628
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 624
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 1 cut(s) 628
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 505
BspTNI GGTCTC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 257
BssMI GATC 4 cut(s) 232, 490, 675, 693
Bst4CI ACNGT 1 cut(s) 664
BstAFI CTTAAG 1 cut(s) 505
BstBAI YACGTR 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 501, 601
BstDEI CTNAG 1 cut(s) 611
BstF5I GGATG 3 cut(s) 76, 194, 544
BstFNI CGCG 1 cut(s) 628
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 2 cut(s) 605, 619
BstKTI GATC 4 cut(s) 235, 493, 678, 696
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 4 cut(s) 232, 490, 675, 693
BstMCI CGRYCG 2 cut(s) 162, 696
BstMWI GCNNNNNNNGC 4 cut(s) 428, 600, 616, 625
BstSCI CCNGG 1 cut(s) 47
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 628
BstV1I GCAGC 3 cut(s) 215, 218, 655
BstXI CCANNNNNNTGG 1 cut(s) 470
Bsu15I ATCGAT 1 cut(s) 678
BsuI GTATCC 1 cut(s) 295
BsuRI GGCC 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 678
BtrI CACGTC 2 cut(s) 478, 565
BtsCI GGATG 3 cut(s) 76, 194, 544
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 501, 601
CaiI CAGNNNCTG 2 cut(s) 44, 668
CfoI GCGC 2 cut(s) 605, 619
Cfr13I GGNCC 1 cut(s) 698
ClaI ATCGAT 1 cut(s) 678
Csp6I GTAC 2 cut(s) 250, 331
CviAII CATG 3 cut(s) 236, 272, 634
CviJI RGCY 9 cut(s) 28, 52, 209, 315, 338, 474, 496, 524, 610
CviKI_1 RGCY 9 cut(s) 28, 52, 209, 315, 338, 474, 496, 524, 610
CviQI GTAC 2 cut(s) 250, 331
DdeI CTNAG 1 cut(s) 611
DpnI GATC 4 cut(s) 234, 492, 677, 695
DpnII GATC 4 cut(s) 232, 490, 675, 693
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 472
EciI GGCGGA 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 698
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 30
FaeI CATG 3 cut(s) 239, 275, 637
FaiI YATR 6 cut(s) 56, 212, 237, 273, 589, 635
FaqI GGGAC 3 cut(s) 282, 551, 644
FatI CATG 3 cut(s) 235, 271, 633
FauI CCCGC 2 cut(s) 382, 621
FblI GTMKAC 2 cut(s) 480, 546
Fnu4HI GCNGC 6 cut(s) 204, 207, 457, 497, 592, 669
FokI GGATG 3 cut(s) 83, 181, 551
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 6 cut(s) 204, 207, 457, 497, 592, 669
GlaI GCGC 2 cut(s) 604, 618
GluI GCNGC 6 cut(s) 204, 207, 457, 497, 592, 669
GsaI CCCAGC 1 cut(s) 610
GsuI CTGGAG 1 cut(s) 274
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 1 cut(s) 474
HapII CCGG 2 cut(s) 48, 158
HhaI GCGC 2 cut(s) 605, 619
Hin1II CATG 3 cut(s) 239, 275, 637
Hin6I GCGC 2 cut(s) 603, 617
HinP1I GCGC 2 cut(s) 603, 617
HincII GTYRAC 2 cut(s) 481, 547
HindII GTYRAC 2 cut(s) 481, 547
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 2 cut(s) 354, 680
HpaII CCGG 2 cut(s) 48, 158
HphI GGTGA 3 cut(s) 308, 504, 550
Hpy166II GTNNAC 3 cut(s) 481, 512, 547
Hpy188III TCNNGA 4 cut(s) 358, 416, 536, 684
Hpy8I GTNNAC 3 cut(s) 481, 512, 547
Hpy99I CGWCG 5 cut(s) 482, 485, 551, 628, 713
HpyAV CCTTC 1 cut(s) 379
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 3 cut(s) 322, 477, 564
HpyCH4V TGCA 6 cut(s) 6, 203, 320, 410, 422, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 600, 616, 625
HpyF3I CTNAG 1 cut(s) 611
HpySE526I ACGT 3 cut(s) 322, 477, 564
Hsp92II CATG 3 cut(s) 239, 275, 637
HspAI GCGC 2 cut(s) 603, 617
Kzo9I GATC 4 cut(s) 232, 490, 675, 693
Lsp1109I GCAGC 3 cut(s) 215, 218, 655
LweI GCATC 2 cut(s) 409, 440
MaeII ACGT 3 cut(s) 322, 477, 564
MaeIII GTNAC 1 cut(s) 556
MalI GATC 4 cut(s) 234, 492, 677, 695
MboI GATC 4 cut(s) 232, 490, 675, 693
MboII GAAGA 4 cut(s) 142, 145, 148, 151
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 2 cut(s) 171, 643
MluNI TGGCCA 1 cut(s) 474
MnlI CCTC 4 cut(s) 287, 292, 337, 700
Mox20I TGGCCA 1 cut(s) 474
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 224, 506
Msp20I TGGCCA 1 cut(s) 474
MspCI CTTAAG 1 cut(s) 505
MspI CCGG 2 cut(s) 48, 158
MspR9I CCNGG 1 cut(s) 49
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 4 cut(s) 428, 600, 616, 625
NciI CCSGG 1 cut(s) 49
NdeII GATC 4 cut(s) 232, 490, 675, 693
NlaIII CATG 3 cut(s) 239, 275, 637
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PfeI GAWTC 2 cut(s) 354, 680
PkrI GCNGC 6 cut(s) 205, 208, 458, 498, 593, 670
Ple19I CGATCG 1 cut(s) 696
Ppu21I YACGTR 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 606
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 698
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 2 cut(s) 44, 668
PvuI CGATCG 1 cut(s) 696
RsaI GTAC 2 cut(s) 251, 332
RsaNI GTAC 2 cut(s) 250, 331
SacI GAGCTC 1 cut(s) 30
SalI GTCGAC 2 cut(s) 479, 545
SaqAI TTAA 2 cut(s) 224, 506
SatI GCNGC 6 cut(s) 204, 207, 457, 497, 592, 669
Sau3AI GATC 4 cut(s) 232, 490, 675, 693
Sau96I GGNCC 1 cut(s) 698
ScaI AGTACT 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 1 cut(s) 30
SfaNI GCATC 2 cut(s) 409, 440
SfcI CTRYAG 1 cut(s) 669
SgrDI CGTCGACG 1 cut(s) 479
SinI GGWCC 1 cut(s) 698
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 2 cut(s) 171, 643
SsiI CCGC 8 cut(s) 93, 389, 454, 457, 497, 528, 592, 628
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 47
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 3 cut(s) 325, 480, 567
TaqI TCGA 5 cut(s) 480, 546, 678, 692, 708
TaqII GACCGA 1 cut(s) 686
TasI AATT 2 cut(s) 171, 643
TatI WGTACW 1 cut(s) 249
TauI GCSGC 3 cut(s) 459, 499, 594
TfiI GAWTC 2 cut(s) 354, 680
Tru1I TTAA 2 cut(s) 224, 506
Tru9I TTAA 2 cut(s) 224, 506
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 3 cut(s) 203, 206, 668
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 2 cut(s) 390, 411
TspGWI ACGGA 2 cut(s) 498, 726
Vha464I CTTAAG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 698
XcmI CCANNNNNNNNNTGG 2 cut(s) 467, 532
XmiI GTMKAC 2 cut(s) 480, 546
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.