FvH4_3g21620

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0019211
Physical Location & Seq
Forward (+)
14705546 .. 14706594
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21620.t1

Sequence Viewer

Length: 597 bp
ATGGCCTGGGCCAGTGACTACTGGACAGTGGTGGGCGACACTCTCTGCCATACATATCTTGAGGCCATTATCGAACCTGAAAACCAATTGCTACGCACCATGTTCTTGATGGATTTGAATGCCACAATCAGACGCCTCCAATCTAGTGATGTTCATGAGGAAGAAATCATAGAAGACAACGTCCGCATTAGCGAGAACCAAGACTCCATTGAACTCCAGTATAACCCTAATGACAACTTCCTTCGAGAGCTTCAAGGTGTTGCCCTATCCAAGAAGCTTCAAGAGCTGCAAGTTCCGATTGAGGCTCGTGGCTCCTTGGTGGATACCATACTGTTCAGGGCCTCTGAGTTCGAATGGAAATATCAAGCTAGCCGCAAGATTTTGCGAATGAGGGTAGAGGTTGATCTTTATGTTATTGATGATGACACAGATGGTGGAGTTGAAACATGTGATTATGGTGTGTCTAGGTTTGTACCGGCCAGTGCACTGCCCGTTGAGAAACTTAGGAGGACAAGAGCCGAAGTGTCATCAACAGTTGGTTCGGAAGCAACTCTCATGCCTTGTTCACATCTTTACCATGGGCACTGCATCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

199

Amino Acids

22.65

Weight (kDa)

4.71

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019211)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21620
rosa_laevigata RLG00000035223
rosa_multiflora Rmu_sc0000367.1_g000123
rosa_roxburghii Rroxscaffold_1G00022070
rosa_rugosa Rorug05G0315000
rosa_samantha Rh5CG417200 Rh5DG408200
rosa_wichuraiana Rw5G035940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 184, 373
AcoI YGGCCR 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 1 cut(s) 474
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 5 cut(s) 118, 212, 254, 281, 443
AjnI CCWGG 1 cut(s) 5
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 4 cut(s) 250, 277, 286, 368
AluI AGCT 4 cut(s) 250, 277, 286, 368
Alw21I GWGCWC 1 cut(s) 487
Alw44I GTGCAC 1 cut(s) 483
AoxI GGCC 5 cut(s) 3, 9, 63, 339, 477
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 9, 339
AsuII TTCGAA 1 cut(s) 351
AsuNHI GCTAGC 1 cut(s) 368
BaeGI GKGCMC 2 cut(s) 487, 585
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 273
BccI CCATC 2 cut(s) 103, 425
BciT130I CCWGG 1 cut(s) 7
BciVI GTATCC 1 cut(s) 316
BfaI CTAG 3 cut(s) 144, 369, 465
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 2 cut(s) 287, 373
BlsI GCNGC 2 cut(s) 288, 374
Bme1390I CCNGG 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 9, 339
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 7
BmtI GCTAGC 1 cut(s) 372
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 133
BsaJI CCNNGG 3 cut(s) 6, 315, 577
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 4 cut(s) 12, 26, 217, 480
BseBI CCWGG 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 6, 315, 577
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 4 cut(s) 12, 26, 217, 480
BseSI GKGCMC 2 cut(s) 487, 585
BseXI GCAGC 1 cut(s) 273
BshFI GGCC 5 cut(s) 5, 11, 65, 341, 479
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 1 cut(s) 476
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 5 cut(s) 5, 11, 65, 341, 479
Bsp119I TTCGAA 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 487, 585
Bsp143I GATC 1 cut(s) 403
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 2 cut(s) 184, 373
BspANI GGCC 5 cut(s) 5, 11, 65, 341, 479
BspCNI CTCAG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 313
BspOI GCTAGC 1 cut(s) 372
BspT104I TTCGAA 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 475
BsrI ACTGG 4 cut(s) 12, 26, 217, 480
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 3 cut(s) 6, 315, 577
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 315, 577
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 3 cut(s) 28, 333, 535
BstACI GRCGYC 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 345, 503
BstDSI CCRYGG 1 cut(s) 577
BstKTI GATC 1 cut(s) 406
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 7
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 5
BstSLI GKGCMC 2 cut(s) 487, 585
BstV1I GCAGC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 180
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 5, 11, 65, 341, 479
BtgI CCRYGG 1 cut(s) 577
BtsI GCAGTG 2 cut(s) 485, 583
BtsIMutI CAGTG 5 cut(s) 19, 33, 485, 487, 583
Cac8I GCNNGC 1 cut(s) 370
CciI TCATGA 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 9, 339
CseI GACGC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 473
CviAII CATG 5 cut(s) 100, 155, 447, 556, 578
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 2 cut(s) 345, 503
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 315, 577
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 5
EcoT14I CCWWGG 2 cut(s) 315, 577
ErhI CCWWGG 2 cut(s) 315, 577
FaeI CATG 5 cut(s) 103, 158, 450, 559, 581
FatI CATG 5 cut(s) 99, 154, 446, 555, 577
Fnu4HI GCNGC 2 cut(s) 287, 373
Fsp4HI GCNGC 2 cut(s) 287, 373
FspBI CTAG 3 cut(s) 144, 369, 465
GluI GCNGC 2 cut(s) 287, 373
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 5 cut(s) 5, 11, 65, 341, 479
HapII CCGG 1 cut(s) 476
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 5 cut(s) 103, 158, 450, 559, 581
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 476
Hpy166II GTNNAC 2 cut(s) 485, 566
Hpy188I TCNGA 4 cut(s) 131, 297, 346, 544
Hpy188III TCNNGA 5 cut(s) 59, 106, 155, 245, 281
Hpy8I GTNNAC 2 cut(s) 485, 566
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 3 cut(s) 28, 333, 535
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 289, 485, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 2 cut(s) 345, 503
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 5 cut(s) 103, 158, 450, 559, 581
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 8 cut(s) 7, 19, 25, 90, 230, 322, 489, 493
Lsp1109I GCAGC 1 cut(s) 273
MaeI CTAG 3 cut(s) 144, 369, 465
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 14
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 2 cut(s) 173, 185
MfeI CAATTG 1 cut(s) 86
MhlI GDGCHC 2 cut(s) 487, 585
MluCI AATT 1 cut(s) 86
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 8 cut(s) 55, 146, 151, 295, 352, 384, 391, 501
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 7
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 283
NcoI CCATGG 1 cut(s) 577
NdeII GATC 1 cut(s) 403
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 5 cut(s) 103, 158, 450, 559, 581
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 14
NspI RCATGY 1 cut(s) 450
NspV TTCGAA 1 cut(s) 351
PagI TCATGA 1 cut(s) 154
PciI ACATGT 1 cut(s) 446
PctI GAATGC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 2 cut(s) 288, 374
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PscI ACATGT 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 5
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 2 cut(s) 9, 339
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SatI GCNGC 2 cut(s) 287, 373
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 2 cut(s) 9, 339
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 2 cut(s) 487, 585
SetI ASST 9 cut(s) 79, 183, 252, 259, 279, 288, 370, 402, 470
SfuI TTCGAA 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 86
SsiI CCGC 2 cut(s) 184, 373
SspMI CTAG 3 cut(s) 144, 369, 465
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 2 cut(s) 315, 577
TaaI ACNGT 3 cut(s) 28, 333, 535
TaiI ACGT 1 cut(s) 183
TaqI TCGA 3 cut(s) 72, 244, 351
TasI AATT 1 cut(s) 86
TauI GCSGC 1 cut(s) 375
TscAI CASTG 5 cut(s) 19, 33, 487, 492, 590
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 1 cut(s) 286
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 5 cut(s) 19, 33, 487, 492, 590
Tth111I GACNNNGTC 1 cut(s) 179
VneI GTGCAC 1 cut(s) 483
XceI RCATGY 1 cut(s) 450
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 3 cut(s) 144, 369, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.