RLG00000035223

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019211
Physical Location & Seq
Reverse (-)
63162755 .. 63163288
534 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035223

Sequence Viewer

Length: 534 bp
ATGGCCTTGGCCAGTGACTACTGGAAAGTGGTGGGTGACACTTGCTGCTATACATATCTTGAGGGCATTATCGAACCTGAAATACGGACCATGTTCTTGATGGATTTGAAGGCCACAATCAGACGCCTCCACTCTGGTGATGTTCACGAGGAAGAAATCATAGCAGACAACGTCCGGATTAGCGAGAACCAGGACTCCATTGAACTCCAGTATAACCCCAATGAAAAGTTCCTTCGAGACTTGCAAGGTGTTGCTCTATCCAAGAAGCTTCAAGAGCTGCAAGTTCCGATTGAGGCTCGTAGCTCCTTGGTGAATACCATAATGTTCAAGGCCTCTGACACTGAATGGAAATATCAAGCTAGCCGCAAGATTTTGCGAATGAGGGTAGAGGTTGATCTCTATGTTATTGATGATGACACAGATGGTGACGATGGTACTATTGATGATTATGGTGTGGCTAGGTTTGTGCCGGCAAGTGCACTGCCCATTGAGAAACTCCGGAGGACCAGAGCCGAAGTGCCATCGACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

178

Amino Acids

20.25

Weight (kDa)

4.73

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019211)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21620
rosa_laevigata RLG00000035223
rosa_multiflora Rmu_sc0000367.1_g000123
rosa_roxburghii Rroxscaffold_1G00022070
rosa_rugosa Rorug05G0315000
rosa_samantha Rh5CG417200 Rh5DG408200
rosa_wichuraiana Rw5G035940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 174, 498
AciI CCGC 1 cut(s) 364
AcoI YGGCCR 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 124
AfaI GTAC 1 cut(s) 436
AgsI TTSAA 4 cut(s) 109, 203, 272, 328
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AleI CACNNNNGTG 1 cut(s) 135
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 268, 277, 303, 359, 531
AluI AGCT 5 cut(s) 268, 277, 303, 359, 531
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 477
Aor13HI TCCGGA 2 cut(s) 174, 498
AoxI GGCC 4 cut(s) 3, 9, 111, 330
ApaLI GTGCAC 1 cut(s) 477
ApeKI GCWGC 2 cut(s) 45, 277
AspS9I GGNCC 2 cut(s) 87, 504
AsuHPI GGTGA 4 cut(s) 47, 149, 322, 437
AsuNHI GCTAGC 1 cut(s) 359
AvaII GGWCC 2 cut(s) 87, 504
BaeGI GKGCMC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 11
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 2 cut(s) 32, 264
BccI CCATC 4 cut(s) 94, 416, 425, 529
BciT130I CCWGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 3 cut(s) 360, 459, 532
BisI GCNGC 3 cut(s) 46, 278, 364
BlsI GCNGC 3 cut(s) 47, 279, 365
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 2 cut(s) 87, 504
BmgT120I GGNCC 2 cut(s) 87, 504
BmrFI CCNGG 1 cut(s) 191
BmtI GCTAGC 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 124
BsaJI CCNNGG 2 cut(s) 6, 306
BsaWI WCCGGW 2 cut(s) 174, 498
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 3 cut(s) 12, 26, 208
BseAI TCCGGA 2 cut(s) 174, 498
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 6, 306
BseNI ACTGG 3 cut(s) 12, 26, 208
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 2 cut(s) 32, 264
BshFI GGCC 4 cut(s) 5, 11, 113, 332
BsiHKAI GWGCWC 1 cut(s) 481
BsiSI CCGG 3 cut(s) 175, 470, 499
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 4 cut(s) 5, 11, 113, 332
Bsp1286I GDGCHC 1 cut(s) 481
Bsp13I TCCGGA 2 cut(s) 174, 498
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 1 cut(s) 364
BspANI GGCC 4 cut(s) 5, 11, 113, 332
BspEI TCCGGA 2 cut(s) 174, 498
BspOI GCTAGC 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 469
BsrI ACTGG 3 cut(s) 12, 26, 208
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 2 cut(s) 6, 306
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 6, 306
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 361, 471
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 32, 264
BsuRI GGCC 4 cut(s) 5, 11, 113, 332
BtsI GCAGTG 1 cut(s) 479
BtsIMutI CAGTG 3 cut(s) 19, 339, 479
Cac8I GCNNGC 2 cut(s) 361, 471
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 2 cut(s) 87, 504
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 435
CviAII CATG 1 cut(s) 91
CviQI GTAC 1 cut(s) 435
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 6, 306
Eco147I AGGCCT 1 cut(s) 332
Eco47I GGWCC 2 cut(s) 87, 504
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 2 cut(s) 6, 306
ErhI CCWWGG 2 cut(s) 6, 306
FaeI CATG 1 cut(s) 94
FaiI YATR 8 cut(s) 51, 55, 92, 161, 213, 320, 402, 450
FatI CATG 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 46, 278, 364
Fsp4HI GCNGC 3 cut(s) 46, 278, 364
FspBI CTAG 3 cut(s) 360, 459, 532
GluI GCNGC 3 cut(s) 46, 278, 364
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 4 cut(s) 5, 11, 113, 332
HapII CCGG 3 cut(s) 175, 470, 499
HgaI GACGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 124
Hin1II CATG 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 1 cut(s) 194
HpaII CCGG 3 cut(s) 175, 470, 499
HphI GGTGA 4 cut(s) 47, 149, 322, 437
Hpy166II GTNNAC 2 cut(s) 145, 479
Hpy188I TCNGA 3 cut(s) 122, 288, 337
Hpy188III TCNNGA 7 cut(s) 59, 97, 146, 175, 236, 272, 499
Hpy8I GTNNAC 2 cut(s) 145, 479
HpyAV CCTTC 2 cut(s) 103, 242
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 244, 280, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 124
Hsp92II CATG 1 cut(s) 94
Kpn2I TCCGGA 2 cut(s) 174, 498
KroI GCCGGC 1 cut(s) 469
KroNI GCCGGC 1 cut(s) 471
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 2 cut(s) 32, 264
MaeI CTAG 3 cut(s) 360, 459, 532
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 14, 35, 425
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 1 cut(s) 164
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 11
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 8 cut(s) 55, 137, 142, 286, 343, 375, 382, 495
Mox20I TGGCCA 1 cut(s) 11
MroI TCCGGA 2 cut(s) 174, 498
MroNI GCCGGC 1 cut(s) 469
MscI TGGCCA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 3 cut(s) 175, 470, 499
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 274
NaeI GCCGGC 1 cut(s) 471
NdeII GATC 1 cut(s) 394
NgoMIV GCCGGC 1 cut(s) 469
NheI GCTAGC 1 cut(s) 359
NlaIII CATG 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 14, 35, 425
OliI CACNNNNGTG 1 cut(s) 135
PceI AGGCCT 1 cut(s) 332
PdiI GCCGGC 1 cut(s) 471
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 47, 279, 365
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspPI GGNCC 2 cut(s) 87, 504
PsrI GAACNNNNNNTAC 2 cut(s) 66, 98
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 135
SatI GCNGC 3 cut(s) 46, 278, 364
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 2 cut(s) 87, 504
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 481
SinI GGWCC 2 cut(s) 87, 504
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 1 cut(s) 364
SspMI CTAG 3 cut(s) 360, 459, 532
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 6, 306
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 72, 235, 524
TauI GCSGC 1 cut(s) 366
TscAI CASTG 3 cut(s) 19, 346, 486
TseFI GTSAC 3 cut(s) 14, 35, 425
TseI GCWGC 2 cut(s) 45, 277
Tsp45I GTSAC 3 cut(s) 14, 35, 425
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 3 cut(s) 19, 346, 486
Tth111I GACNNNGTC 1 cut(s) 170
VneI GTGCAC 1 cut(s) 477
VpaK11BI GGWCC 2 cut(s) 87, 504
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XspI CTAG 3 cut(s) 360, 459, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.