Rroxscaffold_1G00022070

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019211
Physical Location & Seq
Forward (+)
27157722 .. 27158321
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022070.1

Sequence Viewer

Length: 600 bp
ATGGCCTTGGCCAGTGACTACTGGAAAGTGGTGGGCGACACTTGCTGCTATACGTATCTTGAGGGCATTAAAGAACCTGAAAACCAGTTGCTACGGACCATGTTCTTGATGGATTTGAAGTCCACAATCAGACGCCTCCACTCTGGTGATGTTCACGAGGAAGAAATCATAGCAGACAACGTCCGGATTAGCGAGAACCAGGACTCCATTGAACTCCAGTATAACCCCAATGAAAAGTTCCTTCGAGACTTGCAAGGTGTTGCTCTATCCAAGAAGCTTCAAGAGCTGCAAGTTCCGATTGAGGCTCGTAGCTCCTTGGTGGATACCATAATGTTCAAGGCCTCTGACACTGAATGGAAATATCAAGCTAGCCGCAAGATTTTGCGAATGAGGGTAGAGATTGATCTCTATGTTATTGATGATGATACAGATGGTGACGATGGTGCTATTGATGATTATGGTGTGGCTAGGTTTGTGGCGGCAAGTGCACTGCCCATTGAGAAACTCGGGAGGACTAGAGCCGAAGTGCCATCGACAATGGGTTCGGAAGCTACTCTCATGCCTTGTTCACATCGTTATAATGGGGGCTGCGTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

199

Amino Acids

22.5

Weight (kDa)

4.77

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019211)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21620
rosa_laevigata RLG00000035223
rosa_multiflora Rmu_sc0000367.1_g000123
rosa_roxburghii Rroxscaffold_1G00022070
rosa_rugosa Rorug05G0315000
rosa_samantha Rh5CG417200 Rh5DG408200
rosa_wichuraiana Rw5G035940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 2 cut(s) 373, 479
AcoI YGGCCR 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 133
AgsI TTSAA 4 cut(s) 118, 212, 281, 337
AjnI CCWGG 1 cut(s) 198
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AleI CACNNNNGTG 1 cut(s) 144
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 5 cut(s) 277, 286, 312, 368, 551
AluI AGCT 5 cut(s) 277, 286, 312, 368, 551
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 1 cut(s) 240
Alw44I GTGCAC 1 cut(s) 486
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 3 cut(s) 3, 9, 339
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 3 cut(s) 45, 286, 588
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 158, 446
AsuNHI GCTAGC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 490
BalI TGGCCA 1 cut(s) 11
BauI CACGAG 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 490
BbvI GCAGC 3 cut(s) 32, 273, 575
BccI CCATC 4 cut(s) 103, 425, 434, 538
BciT130I CCWGG 1 cut(s) 200
BciVI GTATCC 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 3 cut(s) 369, 468, 516
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 5 cut(s) 46, 287, 373, 480, 589
BlsI GCNGC 5 cut(s) 47, 288, 374, 481, 590
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 96
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 200
BmtI GCTAGC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 80
BsaAI YACGTR 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 6, 315
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse1I ACTGG 4 cut(s) 12, 26, 85, 217
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 6, 315
BseNI ACTGG 4 cut(s) 12, 26, 85, 217
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 3 cut(s) 32, 273, 575
BshFI GGCC 3 cut(s) 5, 11, 341
BsiHKAI GWGCWC 1 cut(s) 490
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 240
BsnI GGCC 3 cut(s) 5, 11, 341
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 490
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 2 cut(s) 373, 479
BspANI GGCC 3 cut(s) 5, 11, 341
BspEI TCCGGA 1 cut(s) 183
BspOI GCTAGC 1 cut(s) 372
BsrI ACTGG 4 cut(s) 12, 26, 85, 217
BssECI CCNNGG 2 cut(s) 6, 315
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 2 cut(s) 6, 315
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 133
BstBAI YACGTR 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 370
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 3 cut(s) 42, 283, 485
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 490
BstSNI TACGTA 1 cut(s) 54
BstV1I GCAGC 3 cut(s) 32, 273, 575
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 5, 11, 341
BtsI GCAGTG 1 cut(s) 488
BtsIMutI CAGTG 3 cut(s) 19, 348, 488
Cac8I GCNNGC 1 cut(s) 370
Cfr13I GGNCC 1 cut(s) 96
CseI GACGC 1 cut(s) 141
CviAII CATG 2 cut(s) 100, 559
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 6, 315
Eco147I AGGCCT 1 cut(s) 341
Eco47I GGWCC 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 6, 315
ErhI CCWWGG 2 cut(s) 6, 315
FaeI CATG 2 cut(s) 103, 562
FatI CATG 2 cut(s) 99, 558
Fnu4HI GCNGC 5 cut(s) 46, 287, 373, 480, 589
Fsp4HI GCNGC 5 cut(s) 46, 287, 373, 480, 589
FspBI CTAG 3 cut(s) 369, 468, 516
GluI GCNGC 5 cut(s) 46, 287, 373, 480, 589
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 3 cut(s) 5, 11, 341
HapII CCGG 1 cut(s) 184
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 2 cut(s) 103, 562
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 158, 446
Hpy166II GTNNAC 4 cut(s) 123, 154, 488, 569
Hpy188I TCNGA 4 cut(s) 131, 297, 346, 547
Hpy188III TCNNGA 7 cut(s) 59, 106, 155, 184, 245, 281, 508
Hpy8I GTNNAC 4 cut(s) 123, 154, 488, 569
HpyAV CCTTC 1 cut(s) 251
HpyCH4IV ACGT 2 cut(s) 53, 180
HpyCH4V TGCA 3 cut(s) 253, 289, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 283, 485
HpySE526I ACGT 2 cut(s) 53, 180
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 2 cut(s) 103, 562
Kpn2I TCCGGA 1 cut(s) 183
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 9 cut(s) 7, 25, 90, 98, 129, 185, 197, 212, 230
Lsp1109I GCAGC 3 cut(s) 32, 273, 575
MaeI CTAG 3 cut(s) 369, 468, 516
MaeII ACGT 2 cut(s) 53, 180
MaeIII GTNAC 2 cut(s) 14, 434
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 490
MlsI TGGCCA 1 cut(s) 11
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 7 cut(s) 55, 146, 151, 295, 352, 384, 504
Mox20I TGGCCA 1 cut(s) 11
MroI TCCGGA 1 cut(s) 183
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 42, 283, 485
NdeII GATC 1 cut(s) 403
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 2 cut(s) 103, 562
NmuCI GTSAC 2 cut(s) 14, 434
OliI CACNNNNGTG 1 cut(s) 144
PceI AGGCCT 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 5 cut(s) 47, 288, 374, 481, 590
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Ppu21I YACGTR 1 cut(s) 54
PsiI TTATAA 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspPI GGNCC 1 cut(s) 96
PsyI GACNNNGTC 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 144
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 5 cut(s) 46, 287, 373, 480, 589
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 96
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 490
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SnaBI TACGTA 1 cut(s) 54
SseBI AGGCCT 1 cut(s) 341
SsiI CCGC 2 cut(s) 373, 479
SspMI CTAG 3 cut(s) 369, 468, 516
StuI AGGCCT 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 6, 315
TaiI ACGT 2 cut(s) 56, 183
TaqI TCGA 2 cut(s) 244, 533
TauI GCSGC 2 cut(s) 375, 482
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 3 cut(s) 19, 355, 495
TseFI GTSAC 2 cut(s) 14, 434
TseI GCWGC 3 cut(s) 45, 286, 588
Tsp45I GTSAC 2 cut(s) 14, 434
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 3 cut(s) 19, 355, 495
Tth111I GACNNNGTC 1 cut(s) 179
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 1 cut(s) 96
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 3 cut(s) 369, 468, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.