Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g21853 FvH4_3g21921 FvH4_3g21922 |
| prunus_persica | Prupe.4G025900_v2.0.a1 |
| pyrus_communis | pycom03g15910 |
| rosa_roxburghii | Rroxscaffold_6G00411980 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 285 |
| AciI | CCGC | 2 cut(s) | 283, 285 |
| AclWI | GGATC | 1 cut(s) | 373 |
| AcoI | YGGCCR | 1 cut(s) | 448 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| AgsI | TTSAA | 2 cut(s) | 116, 248 |
| AjnI | CCWGG | 1 cut(s) | 267 |
| AluBI | AGCT | 3 cut(s) | 120, 211, 439 |
| AluI | AGCT | 3 cut(s) | 120, 211, 439 |
| Alw26I | GTCTC | 1 cut(s) | 409 |
| AlwI | GGATC | 1 cut(s) | 373 |
| AoxI | GGCC | 1 cut(s) | 448 |
| ApeKI | GCWGC | 2 cut(s) | 208, 436 |
| AspS9I | GGNCC | 1 cut(s) | 178 |
| AsuC2I | CCSGG | 1 cut(s) | 176 |
| AsuHPI | GGTGA | 3 cut(s) | 55, 94, 257 |
| AvaII | GGWCC | 1 cut(s) | 178 |
| BbsI | GAAGAC | 1 cut(s) | 314 |
| BbvI | GCAGC | 2 cut(s) | 220, 448 |
| BccI | CCATC | 3 cut(s) | 107, 212, 440 |
| BceAI | ACGGC | 1 cut(s) | 435 |
| BciT130I | CCWGG | 1 cut(s) | 269 |
| BciVI | GTATCC | 1 cut(s) | 407 |
| BcnI | CCSGG | 1 cut(s) | 176 |
| BcoDI | GTCTC | 1 cut(s) | 409 |
| BfaI | CTAG | 1 cut(s) | 35 |
| BfuI | GTATCC | 1 cut(s) | 407 |
| BglII | AGATCT | 1 cut(s) | 69 |
| BisI | GCNGC | 2 cut(s) | 209, 437 |
| BlsI | GCNGC | 2 cut(s) | 210, 438 |
| Bme1390I | CCNGG | 2 cut(s) | 176, 269 |
| Bme18I | GGWCC | 1 cut(s) | 178 |
| BmgT120I | GGNCC | 1 cut(s) | 178 |
| BmrFI | CCNGG | 2 cut(s) | 176, 269 |
| BmsI | GCATC | 1 cut(s) | 177 |
| BpiI | GAAGAC | 1 cut(s) | 314 |
| BpuEI | CTTGAG | 3 cut(s) | 128, 233, 461 |
| BpuMI | CCSGG | 1 cut(s) | 176 |
| BsaJI | CCNNGG | 4 cut(s) | 174, 283, 333, 456 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| Bse1I | ACTGG | 1 cut(s) | 94 |
| Bse3DI | GCAATG | 1 cut(s) | 315 |
| BseBI | CCWGG | 1 cut(s) | 269 |
| BseDI | CCNNGG | 4 cut(s) | 174, 283, 333, 456 |
| BseGI | GGATG | 3 cut(s) | 168, 223, 451 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| BseMI | GCAATG | 1 cut(s) | 315 |
| BseMII | CTCAG | 1 cut(s) | 289 |
| BseNI | ACTGG | 1 cut(s) | 94 |
| BseXI | GCAGC | 2 cut(s) | 220, 448 |
| BseYI | CCCAGC | 1 cut(s) | 428 |
| Bsh1236I | CGCG | 1 cut(s) | 285 |
| BshFI | GGCC | 1 cut(s) | 450 |
| BsiSI | CCGG | 1 cut(s) | 176 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| BsmAI | GTCTC | 1 cut(s) | 409 |
| BsnI | GGCC | 1 cut(s) | 450 |
| Bsp143I | GATC | 3 cut(s) | 60, 69, 378 |
| BspACI | CCGC | 2 cut(s) | 283, 285 |
| BspANI | GGCC | 1 cut(s) | 450 |
| BspCNI | CTCAG | 1 cut(s) | 288 |
| BspFNI | CGCG | 1 cut(s) | 285 |
| BspPI | GGATC | 1 cut(s) | 373 |
| BsrDI | GCAATG | 1 cut(s) | 315 |
| BsrI | ACTGG | 1 cut(s) | 94 |
| BssECI | CCNNGG | 4 cut(s) | 174, 283, 333, 456 |
| BssMI | GATC | 3 cut(s) | 60, 69, 378 |
| BssT1I | CCWWGG | 2 cut(s) | 333, 456 |
| Bst2UI | CCWGG | 1 cut(s) | 269 |
| Bst6I | CTCTTC | 1 cut(s) | 267 |
| BstDEI | CTNAG | 1 cut(s) | 275 |
| BstDSI | CCRYGG | 1 cut(s) | 283 |
| BstF5I | GGATG | 3 cut(s) | 168, 223, 451 |
| BstFNI | CGCG | 1 cut(s) | 285 |
| BstKTI | GATC | 3 cut(s) | 63, 72, 381 |
| BstMAI | GTCTC | 1 cut(s) | 409 |
| BstMBI | GATC | 3 cut(s) | 60, 69, 378 |
| BstNI | CCWGG | 1 cut(s) | 269 |
| BstSCI | CCNGG | 2 cut(s) | 174, 267 |
| BstUI | CGCG | 1 cut(s) | 285 |
| BstV1I | GCAGC | 2 cut(s) | 220, 448 |
| BstV2I | GAAGAC | 1 cut(s) | 314 |
| BstX2I | RGATCY | 2 cut(s) | 69, 378 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 334 |
| BstYI | RGATCY | 2 cut(s) | 69, 378 |
| BsuI | GTATCC | 1 cut(s) | 407 |
| BsuRI | GGCC | 1 cut(s) | 450 |
| BtgI | CCRYGG | 1 cut(s) | 283 |
| BtsCI | GGATG | 3 cut(s) | 168, 223, 451 |
| BtsIMutI | CAGTG | 1 cut(s) | 87 |
| Cfr13I | GGNCC | 1 cut(s) | 178 |
| Cfr42I | CCGCGG | 1 cut(s) | 286 |
| CsiI | ACCWGGT | 1 cut(s) | 267 |
| CviJI | RGCY | 7 cut(s) | 120, 146, 190, 211, 439, 450, 461 |
| CviKI_1 | RGCY | 7 cut(s) | 120, 146, 190, 211, 439, 450, 461 |
| DdeI | CTNAG | 1 cut(s) | 275 |
| DpnI | GATC | 3 cut(s) | 62, 71, 380 |
| DpnII | GATC | 3 cut(s) | 60, 69, 378 |
| EaeI | YGGCCR | 1 cut(s) | 448 |
| Eam1104I | CTCTTC | 1 cut(s) | 267 |
| EarI | CTCTTC | 1 cut(s) | 267 |
| Eco130I | CCWWGG | 2 cut(s) | 333, 456 |
| Eco47I | GGWCC | 1 cut(s) | 178 |
| EcoRII | CCWGG | 1 cut(s) | 267 |
| EcoT14I | CCWWGG | 2 cut(s) | 333, 456 |
| ErhI | CCWWGG | 2 cut(s) | 333, 456 |
| FaiI | YATR | 4 cut(s) | 20, 47, 293, 329 |
| Fnu4HI | GCNGC | 2 cut(s) | 209, 437 |
| FokI | GGATG | 3 cut(s) | 155, 230, 458 |
| Fsp4HI | GCNGC | 2 cut(s) | 209, 437 |
| FspBI | CTAG | 1 cut(s) | 35 |
| GluI | GCNGC | 2 cut(s) | 209, 437 |
| GsaI | CCCAGC | 1 cut(s) | 432 |
| HaeIII | GGCC | 1 cut(s) | 450 |
| HapII | CCGG | 1 cut(s) | 176 |
| HincII | GTYRAC | 1 cut(s) | 235 |
| HindII | GTYRAC | 1 cut(s) | 235 |
| HinfI | GANTC | 5 cut(s) | 82, 254, 259, 279, 310 |
| HpaII | CCGG | 1 cut(s) | 176 |
| HphI | GGTGA | 3 cut(s) | 55, 94, 257 |
| Hpy166II | GTNNAC | 2 cut(s) | 10, 235 |
| Hpy188I | TCNGA | 3 cut(s) | 60, 124, 278 |
| Hpy188III | TCNNGA | 1 cut(s) | 79 |
| Hpy8I | GTNNAC | 2 cut(s) | 10, 235 |
| HpyAV | CCTTC | 3 cut(s) | 158, 364, 386 |
| HpyCH4IV | ACGT | 1 cut(s) | 366 |
| HpyCH4V | TGCA | 1 cut(s) | 295 |
| HpyF3I | CTNAG | 1 cut(s) | 275 |
| HpySE526I | ACGT | 1 cut(s) | 366 |
| KspI | CCGCGG | 1 cut(s) | 286 |
| Kzo9I | GATC | 3 cut(s) | 60, 69, 378 |
| LpnPI | CCDG | 8 cut(s) | 75, 186, 189, 254, 281, 414, 417, 422 |
| Lsp1109I | GCAGC | 2 cut(s) | 220, 448 |
| LweI | GCATC | 1 cut(s) | 177 |
| MabI | ACCWGGT | 1 cut(s) | 267 |
| MaeI | CTAG | 1 cut(s) | 35 |
| MaeII | ACGT | 1 cut(s) | 366 |
| MalI | GATC | 3 cut(s) | 62, 71, 380 |
| MboI | GATC | 3 cut(s) | 60, 69, 378 |
| MboII | GAAGA | 3 cut(s) | 64, 254, 319 |
| MflI | RGATCY | 2 cut(s) | 69, 378 |
| MluCI | AATT | 1 cut(s) | 26 |
| MlyI | GAGTC | 4 cut(s) | 76, 253, 273, 304 |
| MnlI | CCTC | 5 cut(s) | 60, 103, 208, 331, 436 |
| MspA1I | CMGCKG | 1 cut(s) | 285 |
| MspI | CCGG | 1 cut(s) | 176 |
| MspR9I | CCNGG | 2 cut(s) | 176, 269 |
| MvaI | CCWGG | 1 cut(s) | 269 |
| MvnI | CGCG | 1 cut(s) | 285 |
| NciI | CCSGG | 1 cut(s) | 176 |
| NdeII | GATC | 3 cut(s) | 60, 69, 378 |
| PfeI | GAWTC | 1 cut(s) | 254 |
| PkrI | GCNGC | 2 cut(s) | 210, 438 |
| PleI | GAGTC | 4 cut(s) | 76, 253, 273, 304 |
| PpsI | GAGTC | 4 cut(s) | 76, 253, 273, 304 |
| Psp6I | CCWGG | 1 cut(s) | 267 |
| PspFI | CCCAGC | 1 cut(s) | 428 |
| PspGI | CCWGG | 1 cut(s) | 267 |
| PspPI | GGNCC | 1 cut(s) | 178 |
| PsuI | RGATCY | 2 cut(s) | 69, 378 |
| SacII | CCGCGG | 1 cut(s) | 286 |
| SatI | GCNGC | 2 cut(s) | 209, 437 |
| Sau3AI | GATC | 3 cut(s) | 60, 69, 378 |
| Sau96I | GGNCC | 1 cut(s) | 178 |
| SchI | GAGTC | 4 cut(s) | 76, 253, 273, 304 |
| ScrFI | CCNGG | 2 cut(s) | 176, 269 |
| SexAI | ACCWGGT | 1 cut(s) | 267 |
| SfaNI | GCATC | 1 cut(s) | 177 |
| Sfr303I | CCGCGG | 1 cut(s) | 286 |
| SgrBI | CCGCGG | 1 cut(s) | 286 |
| SinI | GGWCC | 1 cut(s) | 178 |
| SmlI | CTYRAG | 3 cut(s) | 107, 212, 440 |
| SmoI | CTYRAG | 3 cut(s) | 107, 212, 440 |
| Sse9I | AATT | 1 cut(s) | 26 |
| SsiI | CCGC | 2 cut(s) | 283, 285 |
| SspI | AATATT | 1 cut(s) | 244 |
| SspMI | CTAG | 1 cut(s) | 35 |
| StyD4I | CCNGG | 2 cut(s) | 174, 267 |
| StyI | CCWWGG | 2 cut(s) | 333, 456 |
| TaiI | ACGT | 1 cut(s) | 369 |
| TaqI | TCGA | 1 cut(s) | 257 |
| TasI | AATT | 1 cut(s) | 26 |
| TfiI | GAWTC | 1 cut(s) | 254 |
| TscAI | CASTG | 1 cut(s) | 94 |
| TseI | GCWGC | 2 cut(s) | 208, 436 |
| TspRI | CASTG | 1 cut(s) | 94 |
| VpaK11BI | GGWCC | 1 cut(s) | 178 |
| XspI | CTAG | 1 cut(s) | 35 |