FvH4_3g25890

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009910
Physical Location & Seq
Forward (+)
18777336 .. 18782833
5498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25890.t1

Sequence Viewer

Length: 441 bp
ATGACGATTGGTTCTGCCGGATCTAGTATCGTCGTGCCACGAAATTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGCATCGGAGATGGTACTGTCAGTTATGGAATGGATGATGGAGATGACATTTATATGCGCTCCTGGACGGGTACTATAATTGGCCCACATAATACTGTTCACGAGGGGCGCATATATCAACTCAAATTGTTCTGCGACAAAGATTATCCAGAGAAGCCACCAAGTGTACGATTTCATACACGCATCAAAATGACTTGTGTCAATCATGAAACTGGAGTGGTTGATCCAAAGAAGTTCGGAATGCTGGCAAATTGGCAGCGGGACTTTACCATGGAAAACATATTGATGCAGCTGAAGAAGGAAATGACAGCGTCACACAACCGGAAACTTGTCCAACCACCTGAAGGTACATATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

147

Amino Acids

16.68

Weight (kDa)

6.96

Isoelectric Point (pI)

22.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 44 - 133 2.6e-14 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 340
AclWI GGATC 2 cut(s) 28, 299
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 395
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 4 cut(s) 97, 154, 249, 430
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 1 cut(s) 68
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 1 cut(s) 373
AluI AGCT 1 cut(s) 373
AlwI GGATC 2 cut(s) 28, 299
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 2 cut(s) 337, 370
ApoI RAATTY 1 cut(s) 43
AspLEI GCGC 2 cut(s) 141, 192
AspS9I GGNCC 1 cut(s) 164
BauI CACGAG 1 cut(s) 182
BbvI GCAGC 2 cut(s) 349, 382
BccI CCATC 2 cut(s) 86, 113
BciT130I CCWGG 1 cut(s) 145
BfaI CTAG 1 cut(s) 24
BisI GCNGC 2 cut(s) 338, 371
BlsI GCNGC 2 cut(s) 339, 372
Bme1390I CCNGG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 3 cut(s) 93, 273, 357
BoxI GACNNNNGTC 1 cut(s) 278
BpmI CTGGAG 2 cut(s) 77, 315
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 402
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 349, 382
BshFI GGCC 1 cut(s) 165
BsiSI CCGG 2 cut(s) 18, 403
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 1 cut(s) 165
Bsp143I GATC 2 cut(s) 20, 304
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 1 cut(s) 340
BspANI GGCC 1 cut(s) 165
BspHI TCATGA 1 cut(s) 286
BspPI GGATC 2 cut(s) 28, 299
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 2 cut(s) 20, 304
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 100, 178
BstC8I GCNNGC 1 cut(s) 327
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 121
BstHHI GCGC 2 cut(s) 141, 192
BstKTI GATC 2 cut(s) 23, 307
BstMBI GATC 2 cut(s) 20, 304
BstNI CCWGG 1 cut(s) 145
BstPAI GACNNNNGTC 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 349, 382
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BsuRI GGCC 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 327
CciI TCATGA 1 cut(s) 286
CfoI GCGC 2 cut(s) 141, 192
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 381
Csp6I GTAC 4 cut(s) 96, 153, 248, 429
CviAII CATG 2 cut(s) 287, 352
CviJI RGCY 3 cut(s) 165, 238, 373
CviKI_1 RGCY 3 cut(s) 165, 238, 373
CviQI GTAC 4 cut(s) 96, 153, 248, 429
DpnI GATC 2 cut(s) 22, 306
DpnII GATC 2 cut(s) 20, 304
DraIII CACNNNGTG 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 290, 355
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 286, 351
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 338, 371
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 338, 371
FspBI CTAG 1 cut(s) 24
GlaI GCGC 2 cut(s) 140, 191
GluI GCNGC 2 cut(s) 338, 371
GsuI CTGGAG 2 cut(s) 77, 315
HaeIII GGCC 1 cut(s) 165
HapII CCGG 2 cut(s) 18, 403
HgaI GACGC 1 cut(s) 381
HhaI GCGC 2 cut(s) 141, 192
Hin1II CATG 2 cut(s) 290, 355
Hin6I GCGC 2 cut(s) 139, 190
HinP1I GCGC 2 cut(s) 139, 190
HpaII CCGG 2 cut(s) 18, 403
Hpy166II GTNNAC 2 cut(s) 181, 248
Hpy188I TCNGA 2 cut(s) 89, 320
Hpy188III TCNNGA 3 cut(s) 182, 230, 287
Hpy8I GTNNAC 2 cut(s) 181, 248
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 2 cut(s) 373, 419
HpyCH4III ACNGT 2 cut(s) 100, 178
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 1 cut(s) 370
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 2 cut(s) 290, 355
HspAI GCGC 2 cut(s) 139, 190
Kzo9I GATC 2 cut(s) 20, 304
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 349, 382
LweI GCATC 3 cut(s) 93, 273, 357
MaeI CTAG 1 cut(s) 24
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 393
MalI GATC 2 cut(s) 22, 306
MboI GATC 2 cut(s) 20, 304
MboII GAAGA 1 cut(s) 388
MflI RGATCY 1 cut(s) 20
MluCI AATT 4 cut(s) 43, 159, 206, 331
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 2 cut(s) 52, 178
MslI CAYNNNNRTG 3 cut(s) 134, 269, 365
MspA1I CMGCKG 2 cut(s) 340, 373
MspI CCGG 2 cut(s) 18, 403
MspR9I CCNGG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 1 cut(s) 145
NcoI CCATGG 1 cut(s) 351
NdeII GATC 2 cut(s) 20, 304
NlaIII CATG 2 cut(s) 290, 355
NmuCI GTSAC 1 cut(s) 393
PagI TCATGA 1 cut(s) 286
PctI GAATGC 1 cut(s) 327
PflFI GACNNNGTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 143
PkrI GCNGC 2 cut(s) 339, 372
PshAI GACNNNNGTC 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 20
PsyI GACNNNGTC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 4 cut(s) 97, 154, 249, 430
RsaNI GTAC 4 cut(s) 96, 153, 248, 429
RseI CAYNNNNRTG 3 cut(s) 134, 269, 365
SatI GCNGC 2 cut(s) 338, 371
Sau3AI GATC 2 cut(s) 20, 304
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 145
SetI ASST 5 cut(s) 53, 73, 375, 424, 430
SfaNI GCATC 3 cut(s) 93, 273, 357
SmiMI CAYNNNNRTG 3 cut(s) 134, 269, 365
Sse9I AATT 4 cut(s) 43, 159, 206, 331
SsiI CCGC 1 cut(s) 340
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 100, 178
TaiI ACGT 1 cut(s) 73
TasI AATT 4 cut(s) 43, 159, 206, 331
TseFI GTSAC 1 cut(s) 393
TseI GCWGC 2 cut(s) 337, 370
Tsp45I GTSAC 1 cut(s) 393
TspDTI ATGAA 2 cut(s) 245, 303
Tth111I GACNNNGTC 1 cut(s) 391
XapI RAATTY 1 cut(s) 43
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.