MD03G1151600.v1.1

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009910
Physical Location & Seq
Forward (+)
17131404 .. 17136518
5115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1151600.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACGATTGGTTCAGCCGGATCCAGCATCGTTGTGCCGAGAAATTTTAGGTTGCTGGAAGAACTCGAGCGTGGAGAGAAGGGTATAGGAGATGGTACTGTAAGCTATGGAATGGATGATGGAGATGACATTTACATGCGCTCTTGGACGGGTACCATAATTGGTCCACTCAATACTGTTCATGAAGGACGCATATATCAACTTAAGCTTTTCTGTGACAAAGATTATCCTGAGAAGCCACCAACTGTACGATTTCATACACGCATCAAAATGGCTTGTGTCAACCATGAAACTGGCATGGTTGAAGTAAAGAAGTTTGGAATGCTGGCAAATTGGCAGCGGGAGTTCACCATGGAAAACATACTGATACAGCTGAAGAAGGAAATGGCAGCCTCGTACAACCGGAAGCTTGCCCAACCACCGGAAGGTACATACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

147

Amino Acids

16.67

Weight (kDa)

6.83

Isoelectric Point (pI)

26.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 44 - 133 4.1e-15 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 152
AciI CCGC 1 cut(s) 340
AclWI GGATC 2 cut(s) 15, 28
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 395
AfaI GTAC 5 cut(s) 97, 154, 249, 398, 430
AfiI CCNNNNNNNGG 2 cut(s) 421, 425
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 1 cut(s) 305
AluBI AGCT 4 cut(s) 105, 208, 373, 409
AluI AGCT 4 cut(s) 105, 208, 373, 409
AlwI GGATC 2 cut(s) 15, 28
Ama87I CYCGRG 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 337, 389
ApoI RAATTY 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 164
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 349, 401
BccI CCATC 2 cut(s) 86, 113
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 2 cut(s) 338, 390
BlsI GCNGC 2 cut(s) 339, 391
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 22, 154
BmsI GCATC 2 cut(s) 36, 273
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 2 cut(s) 402, 421
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 425
Bse1I ACTGG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 421, 425
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 349, 401
BshNI GGYRCC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 3 cut(s) 18, 403, 422
BslI CCNNNNNNNGG 2 cut(s) 421, 425
BsmI GAATGC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 65
Bsp143I GATC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 1 cut(s) 340
BspCNI CTCAG 1 cut(s) 223
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 22, 154
BspPI GGATC 2 cut(s) 15, 28
BspT107I GGYRCC 1 cut(s) 152
BspTI CTTAAG 1 cut(s) 203
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 3 cut(s) 100, 178, 247
BstAFI CTTAAG 1 cut(s) 203
BstC8I GCNNGC 2 cut(s) 327, 411
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 121
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 1 cut(s) 23
BstMBI GATC 1 cut(s) 20
BstNSI RCATGY 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 349, 401
BstX2I RGATCY 1 cut(s) 20
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 327, 411
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 198
Csp6I GTAC 5 cut(s) 96, 153, 248, 397, 429
CviAII CATG 5 cut(s) 136, 182, 287, 298, 352
CviJI RGCY 8 cut(s) 17, 105, 208, 238, 275, 373, 392, 409
CviKI_1 RGCY 8 cut(s) 17, 105, 208, 238, 275, 373, 392, 409
CviQI GTAC 5 cut(s) 96, 153, 248, 397, 429
DdeI CTNAG 1 cut(s) 231
DpnI GATC 1 cut(s) 22
DpnII GATC 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 5 cut(s) 139, 185, 290, 301, 355
FatI CATG 5 cut(s) 135, 181, 286, 297, 351
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 338, 390
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 338, 390
GlaI GCGC 1 cut(s) 140
GluI GCNGC 2 cut(s) 338, 390
HapII CCGG 3 cut(s) 18, 403, 422
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 141
Hin1II CATG 5 cut(s) 139, 185, 290, 301, 355
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 206, 407
HpaII CCGG 3 cut(s) 18, 403, 422
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 3 cut(s) 167, 283, 348
Hpy188III TCNNGA 2 cut(s) 182, 230
Hpy8I GTNNAC 3 cut(s) 167, 283, 348
HpyAV CCTTC 4 cut(s) 73, 179, 373, 419
HpyCH4III ACNGT 3 cut(s) 100, 178, 247
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 5 cut(s) 139, 185, 290, 301, 355
HspAI GCGC 1 cut(s) 139
KpnI GGTACC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 20
LpnPI CCDG 8 cut(s) 31, 37, 41, 243, 279, 311, 416, 435
Lsp1109I GCAGC 2 cut(s) 349, 401
LweI GCATC 2 cut(s) 36, 273
MaeIII GTNAC 1 cut(s) 215
MalI GATC 1 cut(s) 22
MboI GATC 1 cut(s) 20
MboII GAAGA 2 cut(s) 71, 388
MflI RGATCY 1 cut(s) 20
MluCI AATT 3 cut(s) 43, 159, 331
MnlI CCTC 1 cut(s) 403
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 3 cut(s) 32, 134, 269
MspA1I CMGCKG 2 cut(s) 340, 373
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 3 cut(s) 18, 403, 422
Mva1269I GAATGC 1 cut(s) 327
NcoI CCATGG 1 cut(s) 351
NdeII GATC 1 cut(s) 20
NlaIII CATG 5 cut(s) 139, 185, 290, 301, 355
NlaIV GGNNCC 2 cut(s) 22, 154
NmeAIII GCCGAG 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 215
NspI RCATGY 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 181
PctI GAATGC 1 cut(s) 327
PkrI GCNGC 2 cut(s) 339, 391
PspN4I GGNNCC 2 cut(s) 22, 154
PspPI GGNCC 1 cut(s) 164
PspXI VCTCGAGB 1 cut(s) 65
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 5 cut(s) 97, 154, 249, 398, 430
RsaNI GTAC 5 cut(s) 96, 153, 248, 397, 429
RseI CAYNNNNRTG 3 cut(s) 32, 134, 269
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 338, 390
Sau3AI GATC 1 cut(s) 20
Sau96I GGNCC 1 cut(s) 164
SetI ASST 6 cut(s) 53, 107, 210, 375, 411, 430
SfaNI GCATC 2 cut(s) 36, 273
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 3 cut(s) 32, 134, 269
SmlI CTYRAG 2 cut(s) 65, 203
SmoI CTYRAG 2 cut(s) 65, 203
Sse9I AATT 3 cut(s) 43, 159, 331
SsiI CCGC 1 cut(s) 340
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 100, 178, 247
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 43, 159, 331
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 2 cut(s) 337, 389
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 4 cut(s) 170, 198, 245, 303
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 43
XceI RCATGY 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.