MD11G1170800.v1.1

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009910
Physical Location & Seq
Reverse (-)
18467971 .. 18483070
15100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1170800.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACGATTGGTTCTGCCGGATCCAGCATCGTTGTGCCGCGAAATTTTAGGTTGCTGGAAGAGCTCGAGCGTGGAGAGAAGGGTATAGGAGATGGAACTGTAAGCTATGGAATGGATGATGGAGATGACATTTACATGCGCTCTTGGACGGGTACCATAATTGGTCCACTCAATACTGTTCATGAAGGACGCATATACCAACTTAAGCTTTTCTGCGACAAAGATTATCCTGAGAAGCCACCAATTGTACGATTTCATACACGAATCAAAATGGCTTGTGTCAACCATGAAACTGGTGTGGTTGAAGCAAAGAAGTTTGGAATTCTGGCAAATTGGCAGCGGGAGTTCACCATGGAAAACATACTGATACAGCTGAAGAAGGAAATGGCAGCCTCGTACAACCGGAAGCTTGCCCAACCACCAGAAGGTACATACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

147

Amino Acids

16.6

Weight (kDa)

6.83

Isoelectric Point (pI)

26.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 44 - 133 2.8e-14 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 152
AccII CGCG 1 cut(s) 40
AciI CCGC 2 cut(s) 38, 340
AclWI GGATC 2 cut(s) 15, 28
AcsI RAATTY 2 cut(s) 43, 321
AcuI CTGAAG 1 cut(s) 395
AfaI GTAC 4 cut(s) 154, 249, 398, 430
AfiI CCNNNNNNNGG 1 cut(s) 425
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 1 cut(s) 305
AluBI AGCT 5 cut(s) 64, 105, 208, 373, 409
AluI AGCT 5 cut(s) 64, 105, 208, 373, 409
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 2 cut(s) 15, 28
Ama87I CYCGRG 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 337, 389
ApoI RAATTY 2 cut(s) 43, 321
Asp718I GGTACC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 164
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 349, 401
BccI CCATC 2 cut(s) 86, 113
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 3 cut(s) 38, 338, 390
BlsI GCNGC 3 cut(s) 39, 339, 391
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 22, 154
BmsI GCATC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 402
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 349, 401
Bsh1236I CGCG 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 18, 403
BslI CCNNNNNNNGG 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 2 cut(s) 38, 340
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 1 cut(s) 40
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 22, 154
BspPI GGATC 2 cut(s) 15, 28
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 152
BspTI CTTAAG 1 cut(s) 203
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 100, 178
Bst6I CTCTTC 1 cut(s) 54
BstAFI CTTAAG 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 121
BstFNI CGCG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 1 cut(s) 23
BstMBI GATC 1 cut(s) 20
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 139
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 349, 401
BstX2I RGATCY 1 cut(s) 20
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 411
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 198
Csp6I GTAC 4 cut(s) 153, 248, 397, 429
CviAII CATG 4 cut(s) 136, 182, 287, 352
CviJI RGCY 8 cut(s) 64, 105, 208, 238, 275, 373, 392, 409
CviKI_1 RGCY 8 cut(s) 64, 105, 208, 238, 275, 373, 392, 409
CviQI GTAC 4 cut(s) 153, 248, 397, 429
DdeI CTNAG 1 cut(s) 231
DpnI GATC 1 cut(s) 22
DpnII GATC 1 cut(s) 20
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 4 cut(s) 139, 185, 290, 355
FatI CATG 4 cut(s) 135, 181, 286, 351
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 38, 338, 390
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 3 cut(s) 38, 338, 390
GlaI GCGC 1 cut(s) 140
GluI GCNGC 3 cut(s) 38, 338, 390
HapII CCGG 2 cut(s) 18, 403
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 141
Hin1II CATG 4 cut(s) 139, 185, 290, 355
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 206, 407
HinfI GANTC 1 cut(s) 264
HpaII CCGG 2 cut(s) 18, 403
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 3 cut(s) 167, 283, 348
Hpy188III TCNNGA 2 cut(s) 182, 230
Hpy8I GTNNAC 3 cut(s) 167, 283, 348
HpyAV CCTTC 4 cut(s) 73, 179, 373, 419
HpyCH4III ACNGT 2 cut(s) 100, 178
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 4 cut(s) 139, 185, 290, 355
HspAI GCGC 1 cut(s) 139
KpnI GGTACC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 20
LguI GCTCTTC 1 cut(s) 54
LpnPI CCDG 8 cut(s) 31, 37, 41, 243, 279, 311, 416, 435
Lsp1109I GCAGC 2 cut(s) 349, 401
LweI GCATC 1 cut(s) 36
MalI GATC 1 cut(s) 22
MboI GATC 1 cut(s) 20
MboII GAAGA 2 cut(s) 71, 388
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 5 cut(s) 43, 159, 243, 321, 331
MnlI CCTC 1 cut(s) 403
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 2 cut(s) 32, 134
MspA1I CMGCKG 2 cut(s) 340, 373
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 2 cut(s) 18, 403
MunI CAATTG 1 cut(s) 243
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 1 cut(s) 61
NcoI CCATGG 1 cut(s) 351
NdeII GATC 1 cut(s) 20
NlaIII CATG 4 cut(s) 139, 185, 290, 355
NlaIV GGNNCC 2 cut(s) 22, 154
NspI RCATGY 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 3 cut(s) 39, 339, 391
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 22, 154
PspPI GGNCC 1 cut(s) 164
PspXI VCTCGAGB 1 cut(s) 65
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 4 cut(s) 154, 249, 398, 430
RsaNI GTAC 4 cut(s) 153, 248, 397, 429
RseI CAYNNNNRTG 2 cut(s) 32, 134
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 3 cut(s) 38, 338, 390
Sau3AI GATC 1 cut(s) 20
Sau96I GGNCC 1 cut(s) 164
SduI GDGCHC 1 cut(s) 66
SetI ASST 7 cut(s) 53, 66, 107, 210, 375, 411, 430
SfaNI GCATC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 65
SmiMI CAYNNNNRTG 2 cut(s) 32, 134
SmlI CTYRAG 2 cut(s) 65, 203
SmoI CTYRAG 2 cut(s) 65, 203
Sse9I AATT 5 cut(s) 43, 159, 243, 321, 331
SsiI CCGC 2 cut(s) 38, 340
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 100, 178
TaqI TCGA 1 cut(s) 66
TasI AATT 5 cut(s) 43, 159, 243, 321, 331
TauI GCSGC 1 cut(s) 40
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseI GCWGC 2 cut(s) 337, 389
TspDTI ATGAA 4 cut(s) 170, 198, 245, 303
Vha464I CTTAAG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 43, 321
XceI RCATGY 1 cut(s) 139
XhoI CTCGAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.