RchiOBHm_Chr4g0405771

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009910
Physical Location & Seq
Forward (+)
26507694 .. 26508362
669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37723

Sequence Viewer

Length: 240 bp
ATGCTCATGGTTTGCTCGTTGAATTTGTTTCAGACTGTTCATGAAGGGTGCATATATCAACTAAAACTGTTCTATGACAAAGATTATCCAGAGAAGCCACCAAGTGTACGATTTCATACACGCATCAAGATGACTTGTGTCAATCATGAAACTGGAGTGGTTGATTCAAAGAAGTTTGGAATGCTGGCAAATTGGCAGCGGGATTTCACCATGGAAAACATATTGATGCAGTTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

79

Amino Acids

9.39

Weight (kDa)

8.51

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 11 - 78 4.1e-09 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 199
AcsI RAATTY 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 1 cut(s) 108
AgsI TTSAA 3 cut(s) 22, 168, 235
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 199
BbvI GCAGC 1 cut(s) 208
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
BmsI GCATC 2 cut(s) 132, 216
BoxI GACNNNNGTC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 157
BseXI GCAGC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 186
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 1 cut(s) 199
BspHI TCATGA 2 cut(s) 40, 145
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 37, 69
BstC8I GCNNGC 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 210
BstPAI GACNNNNGTC 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 186
CciI TCATGA 2 cut(s) 40, 145
Csp6I GTAC 1 cut(s) 107
CviAII CATG 4 cut(s) 7, 41, 146, 211
CviJI RGCY 1 cut(s) 97
CviKI_1 RGCY 1 cut(s) 97
CviQI GTAC 1 cut(s) 107
DraIII CACNNNGTG 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 4 cut(s) 10, 44, 149, 214
FaiI YATR 9 cut(s) 8, 42, 53, 55, 75, 117, 147, 212, 221
FatI CATG 4 cut(s) 6, 40, 145, 210
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 197
GsuI CTGGAG 1 cut(s) 174
Hin1II CATG 4 cut(s) 10, 44, 149, 214
HinfI GANTC 1 cut(s) 164
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 4 cut(s) 41, 89, 127, 146
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 2 cut(s) 37, 69
HpyCH4V TGCA 2 cut(s) 51, 229
Hsp92II CATG 4 cut(s) 10, 44, 149, 214
LpnPI CCDG 3 cut(s) 102, 138, 170
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 2 cut(s) 132, 216
MluCI AATT 2 cut(s) 22, 190
MslI CAYNNNNRTG 2 cut(s) 128, 224
MspA1I CMGCKG 1 cut(s) 199
Mva1269I GAATGC 1 cut(s) 186
NcoI CCATGG 1 cut(s) 210
NlaIII CATG 4 cut(s) 10, 44, 149, 214
PagI TCATGA 2 cut(s) 40, 145
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 198
PshAI GACNNNNGTC 1 cut(s) 137
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 2 cut(s) 128, 224
SatI GCNGC 1 cut(s) 197
SfaNI GCATC 2 cut(s) 132, 216
SmiMI CAYNNNNRTG 2 cut(s) 128, 224
Sse9I AATT 2 cut(s) 22, 190
SsiI CCGC 1 cut(s) 199
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 2 cut(s) 37, 69
TasI AATT 2 cut(s) 22, 190
TfiI GAWTC 1 cut(s) 164
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 4 cut(s) 29, 57, 104, 162
XapI RAATTY 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.