Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0008775

Physical Location & Seq

Genomic Coordinates Forward (+)

33302188 .. 33310757

Gene Structure 8570 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g39080.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGGGCACCGGCGCGTCCTCCTCCTCCCACGGCGGCGGCGACGAGCCCGAGGAGGAGGAAGAGTACCTCGACCAAGGCGGCGGCGGCCAACTCTACGTCTCCTTGAAGATGGAGAACAATTACAAGCTCAAGAAAGGCGAGCTCCTCCCTCACGTCTTCGGCTCCGTCCCTCTCGTCGGCTCCTGGGACTCCTCCAAAGCCCTTTCTATGGAACGTGAATCCACCTCCATGTGGGAGCTCAGCTTCGTCGTTCCTCCCAATCACGAGACTCTGGATTTTAAGTTCCTGTTGAAGTCCAAGTACAACAGCAATGCACTATGTGTGGTGGAGGAAGGTCCGAATCGCCAGCTCATCGGGGGAGCCTTGCAGGGGGACGCTAGGCAGGCTCTGTTTAGGATTAGTAGTGATGAGGTGCTTGAGTATCGAGTGTTTATTAAGGCGGATAGAGTTTCGCCTTTTGATCTTGCAGCTAGTTGGAGGGCTTATCAGGAGAATCTCCGGCCGTCTGCGGTTCGGGGGATTCCTGATGTCAGTATAGGCTCAGTGCCGGAGACCGGTGGCGAGAATGGCTCTTCTTCAGCGAGTTTGGAGCTTGATCTCGAACATTATGTTGTTCCAGCTCCTCCGTCTTCTGGTCCATTTTATGCAGCTAACAATACAGAGACTCCAAGGTCATTAAGTCTTACTGGGGTCTTTACTAAATCCGATGGCTCTGTCAATTCCTCATATACCTACAAGGATAGCGGTATCCCTATTGATCGATCAAGTAAAAAGGAGATGGAGGTTGTTATTCCAGATTTATCAAAGATGTATTCTTCCTCTGGGATGGTTGAATCAAAGTCCGTGGGAACATTCTCACCTTTCCAAAAGCAAGACAGTCAAAGGGGTCTCTTTGTGGATAGAGGTGTTGGGTCTCCTAGGCTAGTTAAATCATCCAGCTCCAGTGCATTCTCTCCCGAGGTCAGGAAAGAGAGCCCAGAAACAAAGAACTCAATGCCAGAAGCTGCTGGAGCTGTTGCAGCTGCAGCTGTAGCTGATCAAATGCTTGGACCAAAGGAAGATGTGCATTTGGCAATTGTCCTGGTTGGTTTGCCAGCTCGTGGAAAAACATTTACGGCAGCTAAACTTACAAGGTATCTTCGATGGTTGGGTCATGATACCAAACACTTCAATGTTGGAAAGTACCGCCGTCTTAAGCATGGGACCAATCAGACTGCTGACTTTTTCCGAGCTGACAACCCCGAAGGCATGGAGGCACGTAACGAGGTAGCAGCCTTGGCATTTGATGACATGGTTTCTTGGATGCAAGAAGGTGGCCAGGTAGGAATATTTGATGCCACAAACAGTACAAAGAAACGGAGGAATATGCTCATGAAAATGGCTGAAGGAAAATGCAAGATTATCTTTCTGGAAACAATATGCAATGATGAACGAATTATAGAAAGAAATATACGTCTTAAAATTCAACAAAGTCCTGACTATGCAGAAGAGCCAGATTTTGAGGCTGGATGCAAGGACTTCAAAGATCGACTTTCTTATTATGAAAAAGTTTATGATCCAGTAGAAGAAGGGTCGTATATCAAGATGATTGACATGGTCAGTGGACATGGGGGTCAAATACAAGTGAACAACATCAGTGGGTATCTTCCTGGACGAATTGTTTTTTTCCTGGTGAATACACATCTCACACCCCGGCCAATCTTACTTACAAGGCATGGTGAGAGCATGGATAATCTCAGAGGAAGAATAGGTGGAGACCATGCTTTGAGTGATGCCGGAGAACTTTATGCCAAGAAACTTGGTAACTTTGTTGAAAAGCGATTGAAATCAGAGCGAGCTGCTTCGATTTGGACAAGCACGCTGGAGCGAACAATTCTGACAGCAGGTCCCATTTCAGGATTTCCCAAGATACAATGGCGTGCACTGGATGAGATATATGCTGGAGTGTGTGATGGAATGACGTATGACGAGATAAAGAAAAACATGCCAGAAGAATACGAGGCGCGTAAAAAAGATAAGCTGAGGTATCGGTATCCTCGTGGAGAATCTTACTTAGATGTTATCCAAAGGCTAGAACCTGTAATCATTGAACTCGAACGACAACGTGCACCTGTTGTAGTGATATCTCACCAGGCTGTTTTGAGAGCATTATATGCTTACTTTGCTGATAGGCCGCTGAAAGAAATTCCGCATATTGAGATGCCGCTTCATACTATAATAGAGATTCAAATGGGAGTTACAGGCGTCCAAGAGAAAAGATACAAGCTGATGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006000 GO:0006002 GO:0006003 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0019318 GO:0019637 GO:0043609 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0065007 GO:0071704 GO:0071944 GO:1901135

KEGG Pathways

Metabolic & Signaling

ko00051 ko04066 ko04152 map00051 map04066 map04152

Pfam Domains

Protein Families

6PF2K CBM_20 His_Phos_1

Protein Analysis

759

Amino Acids

84.39

Weight (kDa)

6.11

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
6PF2K	PF01591	279 - 495	4.3e-83	6-phosphofructo-2-kinase
AAA_33	PF13671	290 - 415	8e-06	AAA domain
His_Phos_1	PF00300	498 - 680	4e-37	Histidine phosphatase superfamily (branch 1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07110
fragaria_vesca	FvH4_3g39080 FvH4_3g39080 FvH4_3g39080 FvH4_3g39080
malus_domestica	MD03G1064800.v1.1 MD11G1068800.v1.1
prunus_persica	Prupe.6G053800_v2.0.a1 Prupe.6G053800_v2.0.a1 Prupe.6G053800_v2.0.a1
pyrus_communis	pycom03g05190 pycom11g05730
rosa_chinensis	RchiOBHm_Chr5g0070401
rosa_laevigata	RLG00000036130
rosa_multiflora	Rmu_sc0007961.1_g000013
rosa_roxburghii	Rroxscaffold_1G00010800
rosa_rugosa	Rorug05G0405600 Rorug05G0405700.1
rosa_samantha	Rh5AG461100 Rh5BG479400 Rh5CG503600 Rh5DG491400
rosa_wichuraiana	Rw5G042980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	670, 1611
Acc36I	ACCTGC	1 cut(s)	1874
AccB1I	GGYRCC	1 cut(s)	5
AccB7I	CCANNNNNTGG	1 cut(s)	1100
AccII	CGCG	2 cut(s)	14, 2005
AclWI	GGATC	1 cut(s)	1550
AcoI	YGGCCR	4 cut(s)	85, 500, 1315, 1694
AcsI	RAATTY	2 cut(s)	1461, 2184
AcuI	CTGAAG	2 cut(s)	561, 1404
AcyI	GRCGYC	1 cut(s)	2244
AdeI	CACNNNGTG	1 cut(s)	320
AfaI	GTAC	4 cut(s)	65, 302, 1184, 1348
AfiI	CCNNNNNNNGG	9 cut(s)	176, 208, 231, 369, 554, 632, 963, 1100, 1895
AflII	CTTAAG	1 cut(s)	1193
AgeI	ACCGGT	1 cut(s)	554
AhdI	GACNNNNNGTC	1 cut(s)	1884
AjiI	CACGTC	1 cut(s)	154
AjnI	CCWGG	6 cut(s)	182, 1080, 1317, 1648, 1668, 2130
AloI	GAACNNNNNNTCC	2 cut(s)	266, 298
Alw21I	GWGCWC	4 cut(s)	144, 240, 1924, 2110
Alw26I	GTCTC	7 cut(s)	103, 260, 545, 656, 893, 918, 1749
Alw44I	GTGCAC	2 cut(s)	1920, 2106
AlwI	GGATC	1 cut(s)	1550
AlwNI	CAGNNNCTG	2 cut(s)	388, 1004
Ama87I	CYCGRG	2 cut(s)	47, 956
AoxI	GGCC	5 cut(s)	85, 500, 1315, 1694, 2171
ApaLI	GTGCAC	2 cut(s)	1920, 2106
ApeKI	GCWGC	9 cut(s)	467, 647, 1004, 1019, 1022, 1025, 1118, 1271, 1838
ApoI	RAATTY	2 cut(s)	1461, 2184
AsiGI	ACCGGT	1 cut(s)	554
AspA2I	CCTAGG	1 cut(s)	917
AspLEI	GCGC	2 cut(s)	14, 2005
AspS9I	GGNCC	5 cut(s)	335, 635, 1049, 1203, 1886
AsuC2I	CCSGG	1 cut(s)	1693
AsuHPI	GGTGA	4 cut(s)	849, 1684, 1730, 2120
AvaI	CYCGRG	2 cut(s)	47, 956
AvaII	GGWCC	5 cut(s)	335, 635, 1049, 1203, 1886
AvrII	CCTAGG	1 cut(s)	917
BaeGI	GKGCMC	3 cut(s)	8, 1924, 2110
BalI	TGGCCA	1 cut(s)	1317
BanI	GGYRCC	1 cut(s)	5
BanII	GRGCYC	4 cut(s)	48, 144, 240, 977
BarI	GAAGNNNNNNTAC	2 cut(s)	284, 316
BauI	CACGAG	3 cut(s)	263, 1098, 2037
BbsI	GAAGAC	2 cut(s)	148, 621
Bbv12I	GWGCWC	4 cut(s)	144, 240, 1924, 2110
BbvCI	CCTCAGC	1 cut(s)	2021
BbvI	GCAGC	9 cut(s)	479, 659, 991, 1009, 1031, 1037, 1130, 1283, 1825
BccI	CCATC	7 cut(s)	103, 701, 772, 820, 1137, 1946, 2263
BceAI	ACGGC	4 cut(s)	46, 487, 1131, 1173
BciT130I	CCWGG	6 cut(s)	184, 1082, 1319, 1650, 1670, 2132
BciVI	GTATCC	2 cut(s)	758, 2043
BclI	TGATCA	1 cut(s)	1036
BcnI	CCSGG	1 cut(s)	1693
BcoDI	GTCTC	7 cut(s)	103, 260, 545, 656, 893, 918, 1749
BfaI	CTAG	5 cut(s)	378, 471, 918, 923, 2072
BfmI	CTRYAG	2 cut(s)	1023, 1029
BfrI	CTTAAG	1 cut(s)	1193
BfuAI	ACCTGC	1 cut(s)	1874
BfuI	GTATCC	2 cut(s)	758, 2043
BlnI	CCTAGG	1 cut(s)	917
BlpI	GCTNAGC	1 cut(s)	239
Bme1390I	CCNGG	7 cut(s)	184, 1082, 1319, 1650, 1670, 1693, 2132
Bme18I	GGWCC	5 cut(s)	335, 635, 1049, 1203, 1886
BmeRI	GACNNNNNGTC	1 cut(s)	1884
BmeT110I	CYCGRG	2 cut(s)	47, 956
BmgBI	CACGTC	1 cut(s)	154
BmgT120I	GGNCC	5 cut(s)	335, 635, 1049, 1203, 1886
BmiI	GGNNCC	6 cut(s)	7, 163, 181, 361, 1204, 1888
BmrFI	CCNGG	7 cut(s)	184, 1082, 1319, 1650, 1670, 1693, 2132
BmrI	ACTGGG	1 cut(s)	696
BmsI	GCATC	5 cut(s)	1293, 1324, 1499, 1762, 2190
BmuI	ACTGGG	1 cut(s)	696
BpiI	GAAGAC	2 cut(s)	148, 621
BplI	GAGNNNNNCTC	2 cut(s)	554, 586
BpmI	CTGGAG	4 cut(s)	925, 1029, 1883, 1962
Bpu10I	CCTNAGC	1 cut(s)	2021
Bpu1102I	GCTNAGC	1 cut(s)	239
BpuEI	CTTGAG	2 cut(s)	113, 437
BpuMI	CCSGG	1 cut(s)	1693
Bsa29I	ATCGAT	1 cut(s)	760
BsaAI	YACGTR	1 cut(s)	1259
BsaBI	GATNNNNATC	1 cut(s)	1825
BsaHI	GRCGYC	1 cut(s)	2244
BsaI	GGTCTC	4 cut(s)	545, 893, 918, 1749
BsaWI	WCCGGW	1 cut(s)	554
BsaXI	ACNNNNNCTCC	4 cut(s)	47, 77, 649, 679
Bsc4I	CCNNNNNNNGG	9 cut(s)	176, 208, 231, 369, 554, 632, 963, 1100, 1895
Bse118I	RCCGGY	2 cut(s)	8, 554
Bse1I	ACTGG	4 cut(s)	691, 942, 1559, 1929
Bse3DI	GCAATG	2 cut(s)	316, 1429
Bse8I	GATNNNNATC	1 cut(s)	1825
BseBI	CCWGG	6 cut(s)	184, 1082, 1319, 1650, 1670, 2132
BseCI	ATCGAT	1 cut(s)	760
BseGI	GGATG	5 cut(s)	831, 932, 1308, 1514, 1933
BseJI	GATNNNNATC	1 cut(s)	1825
BseLI	CCNNNNNNNGG	9 cut(s)	176, 208, 231, 369, 554, 632, 963, 1100, 1895
BseMI	GCAATG	2 cut(s)	316, 1429
BseMII	CTCAG	4 cut(s)	253, 555, 1750, 2012
BseNI	ACTGG	4 cut(s)	691, 942, 1559, 1929
BseRI	GAGGAG	7 cut(s)	10, 13, 65, 68, 134, 181, 612
BseSI	GKGCMC	3 cut(s)	8, 1924, 2110
BseX3I	CGGCCG	1 cut(s)	500
BseXI	GCAGC	9 cut(s)	479, 659, 991, 1009, 1031, 1037, 1130, 1283, 1825
Bsh1236I	CGCG	2 cut(s)	14, 2005
Bsh1285I	CGRYCG	1 cut(s)	503
BshFI	GGCC	5 cut(s)	87, 502, 1317, 1696, 2173
BshNI	GGYRCC	1 cut(s)	5
BshTI	ACCGGT	1 cut(s)	554
BshVI	ATCGAT	1 cut(s)	760
BsiEI	CGRYCG	1 cut(s)	503
BsiHKAI	GWGCWC	4 cut(s)	144, 240, 1924, 2110
BsiHKCI	CYCGRG	2 cut(s)	47, 956
BsiSI	CCGG	6 cut(s)	9, 499, 548, 555, 1693, 1776
BslFI	GGGAC	5 cut(s)	152, 200, 386, 1216, 1872
BslI	CCNNNNNNNGG	9 cut(s)	176, 208, 231, 369, 554, 632, 963, 1100, 1895
BsmAI	GTCTC	7 cut(s)	103, 260, 545, 656, 893, 918, 1749
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	5 cut(s)	152, 200, 386, 1216, 1872
BsmI	GAATGC	1 cut(s)	947
BsnI	GGCC	5 cut(s)	87, 502, 1317, 1696, 2173
Bso31I	GGTCTC	4 cut(s)	545, 893, 918, 1749
BsoBI	CYCGRG	2 cut(s)	47, 956
Bsp1286I	GDGCHC	7 cut(s)	8, 48, 144, 240, 977, 1924, 2110
Bsp143I	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
Bsp1720I	GCTNAGC	1 cut(s)	239
BspANI	GGCC	5 cut(s)	87, 502, 1317, 1696, 2173
BspCNI	CTCAG	4 cut(s)	252, 554, 1749, 2013
BspDI	ATCGAT	1 cut(s)	760
BspFNI	CGCG	2 cut(s)	14, 2005
BspHI	TCATGA	2 cut(s)	1153, 1371
BspLI	GGNNCC	6 cut(s)	7, 163, 181, 361, 1204, 1888
BspMAI	CTGCAG	1 cut(s)	1027
BspMI	ACCTGC	1 cut(s)	1874
BspPI	GGATC	1 cut(s)	1550
BspQI	GCTCTTC	2 cut(s)	577, 1482
BspT107I	GGYRCC	1 cut(s)	5
BspTI	CTTAAG	1 cut(s)	1193
BspTNI	GGTCTC	4 cut(s)	545, 893, 918, 1749
BsrDI	GCAATG	2 cut(s)	316, 1429
BsrFI	RCCGGY	2 cut(s)	8, 554
BsrI	ACTGG	4 cut(s)	691, 942, 1559, 1929
BssAI	RCCGGY	2 cut(s)	8, 554
BssMI	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
BssNI	GRCGYC	1 cut(s)	2244
BssSI	CACGAG	3 cut(s)	263, 1098, 2037
BssT1I	CCWWGG	4 cut(s)	73, 668, 917, 1275
Bst2BI	CACGAG	3 cut(s)	263, 1098, 2037
Bst2UI	CCWGG	6 cut(s)	184, 1082, 1319, 1650, 1670, 2132
Bst4CI	ACNGT	2 cut(s)	878, 1346
Bst6I	CTCTTC	3 cut(s)	54, 577, 1482
BstACI	GRCGYC	1 cut(s)	2244
BstAFI	CTTAAG	1 cut(s)	1193
BstAPI	GCANNNNNTGC	1 cut(s)	2153
BstBAI	YACGTR	1 cut(s)	1259
BstC8I	GCNNGC	7 cut(s)	140, 347, 384, 1095, 1836, 1859, 1920
BstDEI	CTNAG	5 cut(s)	239, 541, 1736, 2021, 2053
BstDSI	CCRYGG	2 cut(s)	28, 843
BstF5I	GGATG	5 cut(s)	831, 932, 1308, 1514, 1933
BstFNI	CGCG	2 cut(s)	14, 2005
BstHHI	GCGC	2 cut(s)	14, 2005
BstKTI	GATC	7 cut(s)	463, 598, 760, 764, 1039, 1528, 1558
BstMAI	GTCTC	7 cut(s)	103, 260, 545, 656, 893, 918, 1749
BstMBI	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
BstMCI	CGRYCG	1 cut(s)	503
BstNI	CCWGG	6 cut(s)	184, 1082, 1319, 1650, 1670, 2132
BstNSI	RCATGY	1 cut(s)	1987
BstSCI	CCNGG	7 cut(s)	182, 1080, 1317, 1648, 1668, 1691, 2130
BstSFI	CTRYAG	2 cut(s)	1023, 1029
BstSLI	GKGCMC	3 cut(s)	8, 1924, 2110
BstUI	CGCG	2 cut(s)	14, 2005
BstV1I	GCAGC	9 cut(s)	479, 659, 991, 1009, 1031, 1037, 1130, 1283, 1825
BstV2I	GAAGAC	2 cut(s)	148, 621
BstZI	CGGCCG	1 cut(s)	500
Bsu15I	ATCGAT	1 cut(s)	760
BsuI	GTATCC	2 cut(s)	758, 2043
BsuRI	GGCC	5 cut(s)	87, 502, 1317, 1696, 2173
BsuTUI	ATCGAT	1 cut(s)	760
BtgI	CCRYGG	2 cut(s)	28, 843
BtrI	CACGTC	1 cut(s)	154
BtsCI	GGATG	5 cut(s)	831, 932, 1308, 1514, 1933
BtsIMutI	CAGTG	5 cut(s)	549, 949, 1606, 1642, 1922
BveI	ACCTGC	1 cut(s)	1874
Cac8I	GCNNGC	7 cut(s)	140, 347, 384, 1095, 1836, 1859, 1920
CaiI	CAGNNNCTG	2 cut(s)	388, 1004
CciI	TCATGA	2 cut(s)	1153, 1371
CfoI	GCGC	2 cut(s)	14, 2005
Cfr10I	RCCGGY	2 cut(s)	8, 554
Cfr13I	GGNCC	5 cut(s)	335, 635, 1049, 1203, 1886
ClaI	ATCGAT	1 cut(s)	760
CseI	GACGC	3 cut(s)	3, 383, 2233
Csp6I	GTAC	4 cut(s)	64, 301, 1183, 1347
CspAI	ACCGGT	1 cut(s)	554
CspCI	CAANNNNNGTGG	4 cut(s)	825, 860, 1618, 1653
CviQI	GTAC	4 cut(s)	64, 301, 1183, 1347
DdeI	CTNAG	5 cut(s)	239, 541, 1736, 2021, 2053
DpnI	GATC	7 cut(s)	462, 597, 759, 763, 1038, 1527, 1557
DpnII	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
DraIII	CACNNNGTG	1 cut(s)	320
DrdI	GACNNNNNNGTC	2 cut(s)	670, 1611
DriI	GACNNNNNGTC	1 cut(s)	1884
DseDI	GACNNNNNNGTC	2 cut(s)	670, 1611
EaeI	YGGCCR	4 cut(s)	85, 500, 1315, 1694
EagI	CGGCCG	1 cut(s)	500
Eam1104I	CTCTTC	3 cut(s)	54, 577, 1482
Eam1105I	GACNNNNNGTC	1 cut(s)	1884
EarI	CTCTTC	3 cut(s)	54, 577, 1482
EciI	GGCGGA	1 cut(s)	455
Ecl136II	GAGCTC	2 cut(s)	142, 238
EclXI	CGGCCG	1 cut(s)	500
Eco130I	CCWWGG	4 cut(s)	73, 668, 917, 1275
Eco24I	GRGCYC	4 cut(s)	48, 144, 240, 977
Eco31I	GGTCTC	4 cut(s)	545, 893, 918, 1749
Eco32I	GATATC	1 cut(s)	2124
Eco47I	GGWCC	5 cut(s)	335, 635, 1049, 1203, 1886
Eco52I	CGGCCG	1 cut(s)	500
Eco53kI	GAGCTC	2 cut(s)	142, 238
Eco57I	CTGAAG	2 cut(s)	561, 1404
Eco88I	CYCGRG	2 cut(s)	47, 956
EcoICRI	GAGCTC	2 cut(s)	142, 238
EcoO109I	RGGNCCY	1 cut(s)	1886
EcoRII	CCWGG	6 cut(s)	182, 1080, 1317, 1648, 1668, 2130
EcoRV	GATATC	1 cut(s)	2124
EcoT14I	CCWWGG	4 cut(s)	73, 668, 917, 1275
EcoT38I	GRGCYC	4 cut(s)	48, 144, 240, 977
ErhI	CCWWGG	4 cut(s)	73, 668, 917, 1275
Esp3I	CGTCTC	1 cut(s)	103
FalI	AAGNNNNNCTT	4 cut(s)	1388, 1420, 1515, 1547
FaqI	GGGAC	5 cut(s)	152, 200, 386, 1216, 1872
FbaI	TGATCA	1 cut(s)	1036
FokI	GGATG	5 cut(s)	838, 919, 1315, 1521, 1940
FriOI	GRGCYC	4 cut(s)	48, 144, 240, 977
FspBI	CTAG	5 cut(s)	378, 471, 918, 923, 2072
GlaI	GCGC	2 cut(s)	13, 2004
GsuI	CTGGAG	4 cut(s)	925, 1029, 1883, 1962
HaeIII	GGCC	5 cut(s)	87, 502, 1317, 1696, 2173
HapII	CCGG	6 cut(s)	9, 499, 548, 555, 1693, 1776
HgaI	GACGC	3 cut(s)	3, 383, 2233
HhaI	GCGC	2 cut(s)	14, 2005
Hin1I	GRCGYC	1 cut(s)	2244
Hin6I	GCGC	2 cut(s)	12, 2003
HinP1I	GCGC	2 cut(s)	12, 2003
HpaII	CCGG	6 cut(s)	9, 499, 548, 555, 1693, 1776
HphI	GGTGA	4 cut(s)	849, 1684, 1730, 2120
Hpy166II	GTNNAC	4 cut(s)	1604, 1627, 1922, 2108
Hpy188I	TCNGA	7 cut(s)	339, 706, 1212, 1229, 1739, 1831, 1878
Hpy8I	GTNNAC	4 cut(s)	1604, 1627, 1922, 2108
Hpy99I	CGWCG	3 cut(s)	44, 179, 251
HpyAV	CCTTC	5 cut(s)	326, 1238, 1304, 1379, 1562
HpyCH4III	ACNGT	2 cut(s)	878, 1346
HpyCH4IV	ACGT	7 cut(s)	96, 153, 214, 1258, 1453, 1961, 2104
HpyF3I	CTNAG	5 cut(s)	239, 541, 1736, 2021, 2053
HpySE526I	ACGT	7 cut(s)	96, 153, 214, 1258, 1453, 1961, 2104
Hsp92I	GRCGYC	1 cut(s)	2244
HspAI	GCGC	2 cut(s)	12, 2003
Ksp22I	TGATCA	1 cut(s)	1036
Kzo9I	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
LguI	GCTCTTC	2 cut(s)	577, 1482
Lsp1109I	GCAGC	9 cut(s)	479, 659, 991, 1009, 1031, 1037, 1130, 1283, 1825
LweI	GCATC	5 cut(s)	1293, 1324, 1499, 1762, 2190
MaeI	CTAG	5 cut(s)	378, 471, 918, 923, 2072
MaeII	ACGT	7 cut(s)	96, 153, 214, 1258, 1453, 1961, 2104
MaeIII	GTNAC	3 cut(s)	1259, 1802, 2236
MalI	GATC	7 cut(s)	462, 597, 759, 763, 1038, 1527, 1557
MboI	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
MfeI	CAATTG	1 cut(s)	1074
MhlI	GDGCHC	7 cut(s)	8, 48, 144, 240, 977, 1924, 2110
MlsI	TGGCCA	1 cut(s)	1317
MluCI	AATT	8 cut(s)	118, 718, 1074, 1434, 1461, 1656, 1872, 2184
MluNI	TGGCCA	1 cut(s)	1317
MlyI	GAGTC	3 cut(s)	182, 262, 658
MmeI	TCCRAC	2 cut(s)	455, 1156
Mox20I	TGGCCA	1 cut(s)	1317
MscI	TGGCCA	1 cut(s)	1317
MseI	TTAA	6 cut(s)	279, 435, 677, 927, 1194, 1458
MslI	CAYNNNNRTG	3 cut(s)	227, 1170, 1376
Msp20I	TGGCCA	1 cut(s)	1317
MspA1I	CMGCKG	3 cut(s)	1022, 1028, 2176
MspCI	CTTAAG	1 cut(s)	1193
MspI	CCGG	6 cut(s)	9, 499, 548, 555, 1693, 1776
MspR9I	CCNGG	7 cut(s)	184, 1082, 1319, 1650, 1670, 1693, 2132
MunI	CAATTG	1 cut(s)	1074
Mva1269I	GAATGC	1 cut(s)	947
MvaI	CCWGG	6 cut(s)	184, 1082, 1319, 1650, 1670, 2132
MvnI	CGCG	2 cut(s)	14, 2005
NciI	CCSGG	1 cut(s)	1693
NdeII	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
NlaIV	GGNNCC	6 cut(s)	7, 163, 181, 361, 1204, 1888
NspI	RCATGY	1 cut(s)	1987
PagI	TCATGA	2 cut(s)	1153, 1371
PciSI	GCTCTTC	2 cut(s)	577, 1482
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 2035
PctI	GAATGC	1 cut(s)	947
PfeI	GAWTC	7 cut(s)	218, 340, 493, 520, 833, 2045, 2224
PflFI	GACNNNGTC	1 cut(s)	1595
PflMI	CCANNNNNTGG	1 cut(s)	1100
PfoI	TCCNGGA	1 cut(s)	1648
PinAI	ACCGGT	1 cut(s)	554
PleI	GAGTC	3 cut(s)	182, 262, 658
PpsI	GAGTC	3 cut(s)	182, 262, 658
Ppu21I	YACGTR	1 cut(s)	1259
PpuMI	RGGWCCY	1 cut(s)	1886
Psp124BI	GAGCTC	2 cut(s)	144, 240
Psp5II	RGGWCCY	1 cut(s)	1886
Psp6I	CCWGG	6 cut(s)	182, 1080, 1317, 1648, 1668, 2130
PspGI	CCWGG	6 cut(s)	182, 1080, 1317, 1648, 1668, 2130
PspN4I	GGNNCC	6 cut(s)	7, 163, 181, 361, 1204, 1888
PspPI	GGNCC	5 cut(s)	335, 635, 1049, 1203, 1886
PspPPI	RGGWCCY	1 cut(s)	1886
PstI	CTGCAG	1 cut(s)	1027
PstNI	CAGNNNCTG	2 cut(s)	388, 1004
PsyI	GACNNNGTC	1 cut(s)	1595
PvuII	CAGCTG	2 cut(s)	1022, 1028
RsaI	GTAC	4 cut(s)	65, 302, 1184, 1348
RsaNI	GTAC	4 cut(s)	64, 301, 1183, 1347
RseI	CAYNNNNRTG	3 cut(s)	227, 1170, 1376
SacI	GAGCTC	2 cut(s)	144, 240
SapI	GCTCTTC	2 cut(s)	577, 1482
SaqAI	TTAA	6 cut(s)	279, 435, 677, 927, 1194, 1458
Sau3AI	GATC	7 cut(s)	460, 595, 757, 761, 1036, 1525, 1555
Sau96I	GGNCC	5 cut(s)	335, 635, 1049, 1203, 1886
SchI	GAGTC	3 cut(s)	182, 262, 658
ScrFI	CCNGG	7 cut(s)	184, 1082, 1319, 1650, 1670, 1693, 2132
SduI	GDGCHC	7 cut(s)	8, 48, 144, 240, 977, 1924, 2110
SfaNI	GCATC	5 cut(s)	1293, 1324, 1499, 1762, 2190
SfcI	CTRYAG	2 cut(s)	1023, 1029
SgrAI	CRCCGGYG	1 cut(s)	8
SinI	GGWCC	5 cut(s)	335, 635, 1049, 1203, 1886
SmiMI	CAYNNNNRTG	3 cut(s)	227, 1170, 1376
SmlI	CTYRAG	3 cut(s)	128, 416, 1193
SmoI	CTYRAG	3 cut(s)	128, 416, 1193
Sse9I	AATT	8 cut(s)	118, 718, 1074, 1434, 1461, 1656, 1872, 2184
SspI	AATATT	1 cut(s)	1329
SspMI	CTAG	5 cut(s)	378, 471, 918, 923, 2072
SstI	GAGCTC	2 cut(s)	144, 240
StyD4I	CCNGG	7 cut(s)	182, 1080, 1317, 1648, 1668, 1691, 2130
StyI	CCWWGG	4 cut(s)	73, 668, 917, 1275
TaaI	ACNGT	2 cut(s)	878, 1346
TaiI	ACGT	7 cut(s)	99, 156, 217, 1261, 1456, 1964, 2107
TaqI	TCGA	8 cut(s)	69, 424, 600, 760, 1141, 1528, 1844, 2094
TasI	AATT	8 cut(s)	118, 718, 1074, 1434, 1461, 1656, 1872, 2184
TatI	WGTACW	2 cut(s)	300, 1346
TauI	GCSGC	7 cut(s)	36, 39, 81, 84, 87, 2176, 2206
TfiI	GAWTC	7 cut(s)	218, 340, 493, 520, 833, 2045, 2224
Tru1I	TTAA	6 cut(s)	279, 435, 677, 927, 1194, 1458
Tru9I	TTAA	6 cut(s)	279, 435, 677, 927, 1194, 1458
TscAI	CASTG	5 cut(s)	549, 949, 1606, 1642, 1929
TseI	GCWGC	9 cut(s)	467, 647, 1004, 1019, 1022, 1025, 1118, 1271, 1838
TspDTI	ATGAA	4 cut(s)	1388, 1443, 1557, 2198
TspGWI	ACGGA	4 cut(s)	154, 615, 832, 1372
TspRI	CASTG	5 cut(s)	549, 949, 1606, 1642, 1929
Tth111I	GACNNNGTC	1 cut(s)	1595
Van91I	CCANNNNNTGG	1 cut(s)	1100
Vha464I	CTTAAG	1 cut(s)	1193
VneI	GTGCAC	2 cut(s)	1920, 2106
VpaK11BI	GGWCC	5 cut(s)	335, 635, 1049, 1203, 1886
XapI	RAATTY	2 cut(s)	1461, 2184
XceI	RCATGY	1 cut(s)	1987
XmaJI	CCTAGG	1 cut(s)	917
XspI	CTAG	5 cut(s)	378, 471, 918, 923, 2072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.