Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008775

Physical Location & Seq

Genomic Coordinates Reverse (-)

5168749 .. 5176349

Gene Structure 7601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1064800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGGGACTGGTGCGTCCAAGAGGACGGATGACGGCTCCCACGGCGGCGAGGAGCCGGAGGAGGAGGAGCATTTGGACCAGGGAGGAGGACAGCTGTACGTGTCGTTGAAGATGGAGAACAATTACAAGCTCAAGAAAAGCGAGCTTCTTCCTCACATCTTCGGCTCCGTCCCGCTCGTCGGCTCTTGGGATGCTTCCAAAGCTCTTTCAATGGAGCGGGAATCGACTTCCATGTGGGAATTGAGCTTTGTTGTTCCTCCGAATCACGAAACTTTGGACTTCAAGTTTCTTTTGAAGCCAAGGAACAACAACGCACCTGCACCTTGTGTTGTGGAGGAGGGTCCAAACCGCCAACTCATTGGAGGGGCCTTGCAAGGGGATCAAAGGCTGGCCATATTTAGGCTTAACAGTGATGAGGTTCTCGAGTACCGGGTGTTCAATAAGGCGGATAGAGTATCGCCGTTTGATCTTGCAGCCAGTTGGAGAGCTTATCAGGATAACCTTAGGCCTTCTGCTGTTCGCGGAATTCCTGATGTCAGTTTAGATACAGTGCCAGAGATAGGTGCTGAGAATGGCTCTTCAGCTAGTTTGGAGCTTGACCTTGAACATTACGTTGTTCCAGCTCCTTCAACTTCTGCCAATTCAGGTCATATGTATGCAGCTAACATGACAGAGACTCCAAGGTCATTAAATCTTTCTGGAATTTTTACCAAAGCAGATGGCTCTACCAGTGCTTCATATACCTTCAAGGATAGTGGTGTTTTATCCGATCGACATGCAACTACAAAGGAGATGGAGGTTGATATCCCAGATTCATCCAAGCTCTATTCTCCCTCTGGGATGGTTGAATCAAAGTCAGTGGGAACGTTTTCACCTTTCCAAAAGCAAGATAGTCACAGGGGTCTCTTTGTGGATAGGGGTGTTGGATCTCCTAGGCTAGTTAAATCTTCTAGTACTAGTACTTTCTCCGAGTCCAGATTTGATACAGAAAAAAAGAATTCGATGCCAGAAGCTGCTGGAGCGGTTGCGGCTGCAGCCGTAGCTGATCAAATGCTTGGACCAAAGGAAGATAAACATTTGGCAATTGTCCTGGTTGGTTTGCCTGCTCGTGGAAAAACTTTTACAGCAGCTAAGCTTACAAGGTATCTCCGCTGGTTGGGTCATGATACCAAGCACTTCAATATTGGAAAGTACCGCCGTCTTAAGCATGGAGCTAATCAGACTGCTGATTTTTTCCGACCTGACAACCCTGAAGGCATGGAGGCACGTAATGAGGTAGCAGCCCTGGCATTTGATGACATGGTTTCTTGGATGCAAGAAGGTGGCCAGGTAGGAATATTTGATGCCACAAACAGTTCAAGGAAACGGAGAAATATGCTCATGAAGTTGGCTGAAGGAAAGTGCAAGATTATTTTTCTGGAAACGTTATGCAACGATGAACGCATTATAGAAAGAAATATACGTCTTAAAATTCAGCAAAGTCCCGATTATGCAGAACAGACAGATTTTGAGGCTGGATTACAGGACTTCAAAAGTCGATTGGCTTATTATGAAAAAGTTTATGAGCCAGTAGAGGAAGGGTCGTACATCAAAATGATTGATATGGTCAGTGGGCATGGAGGCCAAATACAAGTGAACAACATCAGTGGCTATCTTCCTGGACGAATTGTTTTCTTTCTGGTGAATACACACCTCACGCCCCGCCCAATATTACTTACTAGGCATGGAGAGAGTATGGATAATGTTAGAGGCAGAATTGGAGGAGACAATCCCTTGAGTGATACAGGAGAAATTTATGCAAAGAAACTTGCTAACTTTGTTGAAAAGCGACTGAAATCAGAACGTGCTGCTTCTATTTGGACTAGCACGCTGCAGCGAACAATTTTGACAGCGAGTCCCATTGTAGGATTTCCCAAGATACAATGGCGTGCACTGGATGAGATAAATGCTGGAGCCTGCGATGGAATGACGTATGAAGAGATACAGAAAAACATGCCGAACGAAGCACGTAATAAAGACAAACTGAGGTATCGGTATCCTCGTGGAGAATCTTATTTAGATGTTATCCAAAGGCTAGAGCCAGTAATCATTGAACTCGAACGACAACGTGCACCTGTTGTGGTTATATCTCACCAGGCTGTTTTGAGAGCATTATATGCTTACTTTGCTGATAGGCCTCTGAAAGAGGTTCCACACATTGAGATGCCACTTCACACCATCATAGAGATTCAAATGGGAGTTACAGGCGTACAGGAGAAAAGATACAAGCTCATGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006000 GO:0006002 GO:0006003 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0019318 GO:0019637 GO:0043609 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0065007 GO:0071704 GO:0071944 GO:1901135

KEGG Pathways

Metabolic & Signaling

ko00051 ko04066 ko04152 map00051 map04066 map04152

Pfam Domains

Protein Families

6PF2K CBM_20 His_Phos_1

Protein Analysis

760

Amino Acids

84.71

Weight (kDa)

6.28

Isoelectric Point (pI)

43.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
6PF2K	PF01591	351 - 567	1.1e-82	6-phosphofructo-2-kinase
AAA_33	PF13671	362 - 493	2e-06	AAA domain
His_Phos_1	PF00300	570 - 750	4.9e-36	Histidine phosphatase superfamily (branch 1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07110
fragaria_vesca	FvH4_3g39080 FvH4_3g39080 FvH4_3g39080 FvH4_3g39080
malus_domestica	MD03G1064800.v1.1 MD11G1068800.v1.1
prunus_persica	Prupe.6G053800_v2.0.a1 Prupe.6G053800_v2.0.a1 Prupe.6G053800_v2.0.a1
pyrus_communis	pycom03g05190 pycom11g05730
rosa_chinensis	RchiOBHm_Chr5g0070401
rosa_laevigata	RLG00000036130
rosa_multiflora	Rmu_sc0007961.1_g000013
rosa_roxburghii	Rroxscaffold_1G00010800
rosa_rugosa	Rorug05G0405600 Rorug05G0405700.1
rosa_samantha	Rh5AG461100 Rh5BG479400 Rh5CG503600 Rh5DG491400
rosa_wichuraiana	Rw5G042980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	325
AasI	GACNNNNNNGTC	2 cut(s)	13, 682
Acc36I	ACCTGC	1 cut(s)	325
AccBSI	CCGCTC	3 cut(s)	175, 217, 1022
AccII	CGCG	1 cut(s)	522
AclI	AACGTT	2 cut(s)	866, 1424
AclWI	GGATC	2 cut(s)	387, 934
AcoI	YGGCCR	2 cut(s)	390, 1324
AcsI	RAATTY	5 cut(s)	525, 702, 997, 1470, 1791
AcuI	CTGAAG	3 cut(s)	564, 1272, 1413
AdeI	CACNNNGTG	1 cut(s)	326
AfaI	GTAC	7 cut(s)	98, 428, 955, 961, 1193, 1586, 2250
AfiI	CCNNNNNNNGG	7 cut(s)	179, 375, 399, 560, 1109, 1156, 1904
AflII	CTTAAG	1 cut(s)	1202
AflIII	ACRYGT	1 cut(s)	99
AhdI	GACNNNNNGTC	1 cut(s)	1893
AhlI	ACTAGT	1 cut(s)	956
AjnI	CCWGG	6 cut(s)	78, 1089, 1284, 1326, 1657, 2133
Alw21I	GWGCWC	2 cut(s)	1933, 2113
Alw26I	GTCTC	3 cut(s)	668, 908, 1758
Alw44I	GTGCAC	2 cut(s)	1929, 2109
AlwI	GGATC	2 cut(s)	387, 934
AlwNI	CAGNNNCTG	1 cut(s)	1013
Ama87I	CYCGRG	1 cut(s)	422
AoxI	GGCC	6 cut(s)	366, 390, 506, 1324, 1621, 2174
ApaLI	GTGCAC	2 cut(s)	1929, 2109
ApoI	RAATTY	5 cut(s)	525, 702, 997, 1470, 1791
Asp700I	GAANNNNTTC	1 cut(s)	868
AspA2I	CCTAGG	1 cut(s)	932
AspS9I	GGNCC	4 cut(s)	76, 341, 366, 1058
AsuC2I	CCSGG	1 cut(s)	431
AsuHPI	GGTGA	3 cut(s)	864, 1693, 2123
AvaI	CYCGRG	1 cut(s)	422
AvaII	GGWCC	3 cut(s)	76, 341, 1058
AvrII	CCTAGG	1 cut(s)	932
AxyI	CCTNAGG	1 cut(s)	503
BaeGI	GKGCMC	2 cut(s)	1933, 2113
BaeI	ACNNNNGTAYC	2 cut(s)	2012, 2045
BalI	TGGCCA	2 cut(s)	392, 1326
BauI	CACGAG	2 cut(s)	1107, 2040
Bbv12I	GWGCWC	2 cut(s)	1933, 2113
BccI	CCATC	6 cut(s)	106, 713, 787, 835, 1955, 2225
BceAI	ACGGC	5 cut(s)	49, 58, 445, 1022, 1182
BcgI	CGANNNNNNTGC	2 cut(s)	758, 792
BciT130I	CCWGG	6 cut(s)	80, 1091, 1286, 1328, 1659, 2135
BciVI	GTATCC	1 cut(s)	2046
BclI	TGATCA	1 cut(s)	1045
BcnI	CCSGG	1 cut(s)	431
BcoDI	GTCTC	3 cut(s)	668, 908, 1758
BcuI	ACTAGT	1 cut(s)	956
BfaI	CTAG	8 cut(s)	585, 933, 938, 951, 957, 1719, 1863, 2075
BfmI	CTRYAG	2 cut(s)	1032, 1871
BfrI	CTTAAG	1 cut(s)	1202
BfuAI	ACCTGC	1 cut(s)	325
BfuI	GTATCC	1 cut(s)	2046
BlnI	CCTAGG	1 cut(s)	932
BlpI	GCTNAGC	1 cut(s)	1131
BmcAI	AGTACT	2 cut(s)	955, 961
Bme1390I	CCNGG	7 cut(s)	80, 431, 1091, 1286, 1328, 1659, 2135
Bme18I	GGWCC	3 cut(s)	76, 341, 1058
BmeRI	GACNNNNNGTC	1 cut(s)	1893
BmeT110I	CYCGRG	1 cut(s)	422
BmgT120I	GGNCC	4 cut(s)	76, 341, 366, 1058
BmiI	GGNNCC	7 cut(s)	37, 54, 166, 342, 367, 1954, 2190
BmrFI	CCNGG	7 cut(s)	80, 431, 1091, 1286, 1328, 1659, 2135
BmsI	GCATC	5 cut(s)	181, 993, 1302, 1333, 2193
BplI	GAGNNNNNCTC	2 cut(s)	560, 592
BpmI	CTGGAG	2 cut(s)	1038, 1971
Bpu1102I	GCTNAGC	1 cut(s)	1131
BpuEI	CTTGAG	2 cut(s)	116, 1795
BpuMI	CCSGG	1 cut(s)	431
BsaAI	YACGTR	3 cut(s)	100, 1268, 2009
BsaI	GGTCTC	1 cut(s)	908
BsaJI	CCNNGG	6 cut(s)	40, 79, 299, 680, 932, 1284
BsaXI	ACNNNNNCTCC	2 cut(s)	661, 691
Bsc4I	CCNNNNNNNGG	7 cut(s)	179, 375, 399, 560, 1109, 1156, 1904
Bse1I	ACTGG	6 cut(s)	13, 477, 729, 1568, 1938, 2081
Bse21I	CCTNAGG	1 cut(s)	503
BseBI	CCWGG	6 cut(s)	80, 1091, 1286, 1328, 1659, 2135
BseDI	CCNNGG	6 cut(s)	40, 79, 299, 680, 932, 1284
BseGI	GGATG	6 cut(s)	34, 196, 815, 846, 1317, 1942
BseLI	CCNNNNNNNGG	7 cut(s)	179, 375, 399, 560, 1109, 1156, 1904
BseMII	CTCAG	2 cut(s)	558, 2015
BseNI	ACTGG	6 cut(s)	13, 477, 729, 1568, 1938, 2081
BseRI	GAGGAG	7 cut(s)	65, 74, 77, 80, 99, 350, 1776
BseSI	GKGCMC	2 cut(s)	1933, 2113
BsgI	GTGCAG	1 cut(s)	303
Bsh1236I	CGCG	1 cut(s)	522
Bsh1285I	CGRYCG	1 cut(s)	772
BshFI	GGCC	6 cut(s)	368, 392, 508, 1326, 1623, 2176
BsiEI	CGRYCG	1 cut(s)	772
BsiHKAI	GWGCWC	2 cut(s)	1933, 2113
BsiHKCI	CYCGRG	1 cut(s)	422
BsiSI	CCGG	2 cut(s)	56, 430
BslFI	GGGAC	4 cut(s)	19, 155, 1467, 1881
BslI	CCNNNNNNNGG	7 cut(s)	179, 375, 399, 560, 1109, 1156, 1904
BsmAI	GTCTC	3 cut(s)	668, 908, 1758
BsmFI	GGGAC	4 cut(s)	19, 155, 1467, 1881
BsnI	GGCC	6 cut(s)	368, 392, 508, 1326, 1623, 2176
Bso31I	GGTCTC	1 cut(s)	908
BsoBI	CYCGRG	1 cut(s)	422
Bsp1286I	GDGCHC	2 cut(s)	1933, 2113
Bsp143I	GATC	5 cut(s)	379, 466, 769, 926, 1045
Bsp1720I	GCTNAGC	1 cut(s)	1131
BspANI	GGCC	6 cut(s)	368, 392, 508, 1326, 1623, 2176
BspCNI	CTCAG	2 cut(s)	559, 2016
BspFNI	CGCG	1 cut(s)	522
BspHI	TCATGA	2 cut(s)	1162, 1380
BspLI	GGNNCC	7 cut(s)	37, 54, 166, 342, 367, 1954, 2190
BspMAI	CTGCAG	2 cut(s)	1036, 1875
BspMI	ACCTGC	1 cut(s)	325
BspPI	GGATC	2 cut(s)	387, 934
BspQI	GCTCTTC	1 cut(s)	583
BspTI	CTTAAG	1 cut(s)	1202
BspTNI	GGTCTC	1 cut(s)	908
BsrBI	CCGCTC	3 cut(s)	175, 217, 1022
BsrI	ACTGG	6 cut(s)	13, 477, 729, 1568, 1938, 2081
BssECI	CCNNGG	6 cut(s)	40, 79, 299, 680, 932, 1284
BssMI	GATC	5 cut(s)	379, 466, 769, 926, 1045
BssSI	CACGAG	2 cut(s)	1107, 2040
BssT1I	CCWWGG	3 cut(s)	299, 680, 932
Bst2BI	CACGAG	2 cut(s)	1107, 2040
Bst2UI	CCWGG	6 cut(s)	80, 1091, 1286, 1328, 1659, 2135
Bst4CI	ACNGT	3 cut(s)	410, 550, 1355
Bst6I	CTCTTC	2 cut(s)	583, 1971
BstAFI	CTTAAG	1 cut(s)	1202
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstBAI	YACGTR	3 cut(s)	100, 1268, 2009
BstC8I	GCNNGC	6 cut(s)	143, 390, 1104, 1868, 1929, 1957
BstDEI	CTNAG	4 cut(s)	503, 567, 1131, 2024
BstDSI	CCRYGG	1 cut(s)	40
BstF5I	GGATG	6 cut(s)	34, 196, 815, 846, 1317, 1942
BstFNI	CGCG	1 cut(s)	522
BstKTI	GATC	5 cut(s)	382, 469, 772, 929, 1048
BstMAI	GTCTC	3 cut(s)	668, 908, 1758
BstMBI	GATC	5 cut(s)	379, 466, 769, 926, 1045
BstMCI	CGRYCG	1 cut(s)	772
BstMWI	GCNNNNNNNGC	9 cut(s)	42, 200, 1019, 1028, 1034, 1040, 1286, 2156, 2165
BstNI	CCWGG	6 cut(s)	80, 1091, 1286, 1328, 1659, 2135
BstNSI	RCATGY	2 cut(s)	779, 1996
BstSCI	CCNGG	7 cut(s)	78, 429, 1089, 1284, 1326, 1657, 2133
BstSFI	CTRYAG	2 cut(s)	1032, 1871
BstSLI	GKGCMC	2 cut(s)	1933, 2113
BstUI	CGCG	1 cut(s)	522
BstX2I	RGATCY	1 cut(s)	926
BstXI	CCANNNNNNTGG	1 cut(s)	359
BstYI	RGATCY	1 cut(s)	926
Bsu36I	CCTNAGG	1 cut(s)	503
BsuI	GTATCC	1 cut(s)	2046
BsuRI	GGCC	6 cut(s)	368, 392, 508, 1326, 1623, 2176
BtgI	CCRYGG	1 cut(s)	40
BtgZI	GCGATG	1 cut(s)	1974
BtsCI	GGATG	6 cut(s)	34, 196, 815, 846, 1317, 1942
BtsIMutI	CAGTG	7 cut(s)	415, 555, 736, 864, 1615, 1651, 1931
BveI	ACCTGC	1 cut(s)	325
Cac8I	GCNNGC	6 cut(s)	143, 390, 1104, 1868, 1929, 1957
CaiI	CAGNNNCTG	1 cut(s)	1013
CciI	TCATGA	2 cut(s)	1162, 1380
Cfr13I	GGNCC	4 cut(s)	76, 341, 366, 1058
CseI	GACGC	1 cut(s)	3
Csp6I	GTAC	7 cut(s)	97, 427, 954, 960, 1192, 1585, 2249
CspCI	CAANNNNNGTGG	6 cut(s)	736, 771, 840, 875, 1627, 1662
CviQI	GTAC	7 cut(s)	97, 427, 954, 960, 1192, 1585, 2249
DdeI	CTNAG	4 cut(s)	503, 567, 1131, 2024
DpnI	GATC	5 cut(s)	381, 468, 771, 928, 1047
DpnII	GATC	5 cut(s)	379, 466, 769, 926, 1045
DraIII	CACNNNGTG	1 cut(s)	326
DrdI	GACNNNNNNGTC	2 cut(s)	13, 682
DriI	GACNNNNNGTC	1 cut(s)	1893
DseDI	GACNNNNNNGTC	2 cut(s)	13, 682
EaeI	YGGCCR	2 cut(s)	390, 1324
Eam1104I	CTCTTC	2 cut(s)	583, 1971
Eam1105I	GACNNNNNGTC	1 cut(s)	1893
EarI	CTCTTC	2 cut(s)	583, 1971
EciI	GGCGGA	1 cut(s)	461
Eco130I	CCWWGG	3 cut(s)	299, 680, 932
Eco147I	AGGCCT	2 cut(s)	508, 2176
Eco31I	GGTCTC	1 cut(s)	908
Eco32I	GATATC	1 cut(s)	805
Eco47I	GGWCC	3 cut(s)	76, 341, 1058
Eco57I	CTGAAG	3 cut(s)	564, 1272, 1413
Eco81I	CCTNAGG	1 cut(s)	503
Eco88I	CYCGRG	1 cut(s)	422
EcoO109I	RGGNCCY	1 cut(s)	366
EcoRI	GAATTC	2 cut(s)	525, 997
EcoRII	CCWGG	6 cut(s)	78, 1089, 1284, 1326, 1657, 2133
EcoRV	GATATC	1 cut(s)	805
EcoT14I	CCWWGG	3 cut(s)	299, 680, 932
ErhI	CCWWGG	3 cut(s)	299, 680, 932
FaqI	GGGAC	4 cut(s)	19, 155, 1467, 1881
FauI	CCCGC	3 cut(s)	180, 210, 1709
FauNDI	CATATG	1 cut(s)	651
FbaI	TGATCA	1 cut(s)	1045
FokI	GGATG	6 cut(s)	41, 203, 802, 853, 1324, 1949
FspBI	CTAG	8 cut(s)	585, 933, 938, 951, 957, 1719, 1863, 2075
GsuI	CTGGAG	2 cut(s)	1038, 1971
HaeIII	GGCC	6 cut(s)	368, 392, 508, 1326, 1623, 2176
HapII	CCGG	2 cut(s)	56, 430
HgaI	GACGC	1 cut(s)	3
HindIII	AAGCTT	1 cut(s)	1133
HinfI	GANTC	9 cut(s)	221, 262, 676, 812, 848, 971, 1894, 2048, 2227
HpaII	CCGG	2 cut(s)	56, 430
HphI	GGTGA	3 cut(s)	864, 1693, 2123
Hpy166II	GTNNAC	3 cut(s)	1636, 1931, 2111
Hpy188I	TCNGA	7 cut(s)	261, 769, 970, 1221, 1238, 1840, 2181
Hpy8I	GTNNAC	3 cut(s)	1636, 1931, 2111
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	7 cut(s)	519, 636, 754, 1247, 1313, 1388, 1571
HpyCH4III	ACNGT	3 cut(s)	410, 550, 1355
HpyF10VI	GCNNNNNNNGC	9 cut(s)	42, 200, 1019, 1028, 1034, 1040, 1286, 2156, 2165
HpyF3I	CTNAG	4 cut(s)	503, 567, 1131, 2024
Ksp22I	TGATCA	1 cut(s)	1045
Kzo9I	GATC	5 cut(s)	379, 466, 769, 926, 1045
LguI	GCTCTTC	1 cut(s)	583
LweI	GCATC	5 cut(s)	181, 993, 1302, 1333, 2193
MaeI	CTAG	8 cut(s)	585, 933, 938, 951, 957, 1719, 1863, 2075
MaeIII	GTNAC	2 cut(s)	893, 2239
MalI	GATC	5 cut(s)	381, 468, 771, 928, 1047
MbiI	CCGCTC	3 cut(s)	175, 217, 1022
MboI	GATC	5 cut(s)	379, 466, 769, 926, 1045
MboII	GAAGA	8 cut(s)	121, 140, 151, 570, 939, 1079, 1646, 1988
MfeI	CAATTG	1 cut(s)	1083
MflI	RGATCY	1 cut(s)	926
MhlI	GDGCHC	2 cut(s)	1933, 2113
MlsI	TGGCCA	2 cut(s)	392, 1326
MluNI	TGGCCA	2 cut(s)	392, 1326
MlyI	GAGTC	3 cut(s)	670, 980, 1903
MmeI	TCCRAC	3 cut(s)	461, 904, 1261
Mox20I	TGGCCA	2 cut(s)	392, 1326
MroXI	GAANNNNTTC	1 cut(s)	868
MscI	TGGCCA	2 cut(s)	392, 1326
MseI	TTAA	5 cut(s)	405, 689, 942, 1203, 1467
MslI	CAYNNNNRTG	3 cut(s)	654, 1592, 2201
Msp20I	TGGCCA	2 cut(s)	392, 1326
MspA1I	CMGCKG	2 cut(s)	94, 1152
MspCI	CTTAAG	1 cut(s)	1202
MspI	CCGG	2 cut(s)	56, 430
MspR9I	CCNGG	7 cut(s)	80, 431, 1091, 1286, 1328, 1659, 2135
MunI	CAATTG	1 cut(s)	1083
MvaI	CCWGG	6 cut(s)	80, 1091, 1286, 1328, 1659, 2135
MvnI	CGCG	1 cut(s)	522
MwoI	GCNNNNNNNGC	9 cut(s)	42, 200, 1019, 1028, 1034, 1040, 1286, 2156, 2165
NciI	CCSGG	1 cut(s)	431
NdeI	CATATG	1 cut(s)	651
NdeII	GATC	5 cut(s)	379, 466, 769, 926, 1045
NlaIV	GGNNCC	7 cut(s)	37, 54, 166, 342, 367, 1954, 2190
NmuCI	GTSAC	1 cut(s)	893
NspI	RCATGY	2 cut(s)	779, 1996
PaeR7I	CTCGAG	1 cut(s)	422
PagI	TCATGA	2 cut(s)	1162, 1380
PaqCI	CACCTGC	1 cut(s)	325
PceI	AGGCCT	2 cut(s)	508, 2176
PciSI	GCTCTTC	1 cut(s)	583
PcsI	WCGNNNNNNNCGW	1 cut(s)	2038
PdmI	GAANNNNTTC	1 cut(s)	868
PfeI	GAWTC	6 cut(s)	221, 262, 812, 848, 2048, 2227
PfoI	TCCNGGA	1 cut(s)	1657
Ple19I	CGATCG	1 cut(s)	772
PleI	GAGTC	3 cut(s)	670, 979, 1902
PpsI	GAGTC	3 cut(s)	670, 979, 1902
Ppu21I	YACGTR	3 cut(s)	100, 1268, 2009
Psp1406I	AACGTT	2 cut(s)	866, 1424
Psp6I	CCWGG	6 cut(s)	78, 1089, 1284, 1326, 1657, 2133
PspGI	CCWGG	6 cut(s)	78, 1089, 1284, 1326, 1657, 2133
PspN4I	GGNNCC	7 cut(s)	37, 54, 166, 342, 367, 1954, 2190
PspPI	GGNCC	4 cut(s)	76, 341, 366, 1058
PsrI	GAACNNNNNNTAC	2 cut(s)	419, 451
PstI	CTGCAG	2 cut(s)	1036, 1875
PstNI	CAGNNNCTG	1 cut(s)	1013
PsuI	RGATCY	1 cut(s)	926
PvuI	CGATCG	1 cut(s)	772
PvuII	CAGCTG	1 cut(s)	94
RsaI	GTAC	7 cut(s)	98, 428, 955, 961, 1193, 1586, 2250
RsaNI	GTAC	7 cut(s)	97, 427, 954, 960, 1192, 1585, 2249
RseI	CAYNNNNRTG	3 cut(s)	654, 1592, 2201
SapI	GCTCTTC	1 cut(s)	583
SaqAI	TTAA	5 cut(s)	405, 689, 942, 1203, 1467
Sau3AI	GATC	5 cut(s)	379, 466, 769, 926, 1045
Sau96I	GGNCC	4 cut(s)	76, 341, 366, 1058
ScaI	AGTACT	2 cut(s)	955, 961
SchI	GAGTC	3 cut(s)	670, 980, 1903
ScrFI	CCNGG	7 cut(s)	80, 431, 1091, 1286, 1328, 1659, 2135
SduI	GDGCHC	2 cut(s)	1933, 2113
SfaNI	GCATC	5 cut(s)	181, 993, 1302, 1333, 2193
SfcI	CTRYAG	2 cut(s)	1032, 1871
Sfr274I	CTCGAG	1 cut(s)	422
SinI	GGWCC	3 cut(s)	76, 341, 1058
SlaI	CTCGAG	1 cut(s)	422
SmiMI	CAYNNNNRTG	3 cut(s)	654, 1592, 2201
SmlI	CTYRAG	4 cut(s)	131, 422, 1202, 1774
SmoI	CTYRAG	4 cut(s)	131, 422, 1202, 1774
SpeI	ACTAGT	1 cut(s)	956
SseBI	AGGCCT	2 cut(s)	508, 2176
SspI	AATATT	3 cut(s)	1183, 1338, 1710
SspMI	CTAG	8 cut(s)	585, 933, 938, 951, 957, 1719, 1863, 2075
StuI	AGGCCT	2 cut(s)	508, 2176
StyD4I	CCNGG	7 cut(s)	78, 429, 1089, 1284, 1326, 1657, 2133
StyI	CCWWGG	3 cut(s)	299, 680, 932
TaaI	ACNGT	3 cut(s)	410, 550, 1355
TaqI	TCGA	6 cut(s)	224, 423, 772, 1001, 1537, 2097
TatI	WGTACW	2 cut(s)	953, 959
TauI	GCSGC	2 cut(s)	48, 1031
TfiI	GAWTC	6 cut(s)	221, 262, 812, 848, 2048, 2227
Tru1I	TTAA	5 cut(s)	405, 689, 942, 1203, 1467
Tru9I	TTAA	5 cut(s)	405, 689, 942, 1203, 1467
TscAI	CASTG	7 cut(s)	415, 555, 736, 864, 1615, 1651, 1938
TseFI	GTSAC	1 cut(s)	893
Tsp45I	GTSAC	1 cut(s)	893
TspDTI	ATGAA	6 cut(s)	726, 804, 1397, 1452, 1566, 1989
TspGWI	ACGGA	3 cut(s)	41, 157, 1381
TspRI	CASTG	7 cut(s)	415, 555, 736, 864, 1615, 1651, 1938
Vha464I	CTTAAG	1 cut(s)	1202
VneI	GTGCAC	2 cut(s)	1929, 2109
VpaK11BI	GGWCC	3 cut(s)	76, 341, 1058
XapI	RAATTY	5 cut(s)	525, 702, 997, 1470, 1791
XceI	RCATGY	2 cut(s)	779, 1996
XhoI	CTCGAG	1 cut(s)	422
XmaJI	CCTAGG	1 cut(s)	932
XmnI	GAANNNNTTC	1 cut(s)	868
XspI	CTAG	8 cut(s)	585, 933, 938, 951, 957, 1719, 1863, 2075
ZrmI	AGTACT	2 cut(s)	955, 961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.