FvH4_3g43390

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018015
Physical Location & Seq
Forward (+)
36183336 .. 36188898
5563 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43390.t1

Sequence Viewer

Length: 3717 bp
ATGGCGGAGGCGAGGATCGGTTGCATTGCCATTCTTACCAGGCTATGGGATCAAGTGAAGCGTCGGTTCAGTTACATGGTCGAAAATTCGCAGGAGAATCAAGTATTGGTTGACATGGATGCCTCTGCATCTCTTCCTTCTTCATCGTCAGCAGTACTTGAATGCAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGGATTACATTCGACATATACGATCGACTTAATACGAGAGGAATTAAAACATTCATGGATGATAGGGATCTTGAAGTAGGGGATGCTATTTCTCCCACTCTCATAGCAGCAATCAAAGAATCAAGGTTTGCAATTGTTGTTCTCTCGCAAAATTATGCTTTTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTGTCCATGGAAGACAACAGAATTTTACCACTTTTTTATAATGTTGATCCTACTGATGTTCGATATCATAAGAAGGGAAGTTTCAAAGAAGCTTTCTCTAAGCATGTAAAATCTGGGAGACACAGACCAGAGAAGGTGAAGGAGTGGAAAGCTGCGTTAAACAAAGTAGCCAATATCTCTGGGTGGAATACAACTGATCATAAAACTGAGAGAGCACTTACCGAAGCCATTCTGGAACTTCTCAGCAGTAAAATTGTACCTGATGCAATTGAGTCCACTGGAGATTTCCAAGCATTTGAAGCAACAAGAGTAGCCATGGATGAGGTTTGGGATGCCTTTAAAGACAACAAGGTCACTGCCATTGGTGTCTACGGCATGGGAGGCGTTGGCAAGACAACCTTAGTGGAACATGTTGGTGCACAAGCCCGAAAAGGTGGGATTTTCTATTATGTGACAAAAAGTGTCGTGTCCCAGAATCCCGATTTCAAAAAACTTCAAGATACATTGGCAGAGCAGCTGGGCTTCGAATTACCTAAGGAGACAGAAACCGGAAGAGCAGCTAGATTGAAAAAGGAGATAAAGAAAAGGGAAAAAATACTTATAATCTTGGACGACATTTGGGAGAGAACGGACTTATCAAGAATAGGAATTCCAAGCAACAAGGAACTTCAAAATTGCAATTCCAAAGTCGTACTGACCACAAGGATAAGGAATGTTTGTCATGCCATGGAGTGCCAAAGAAAGATCACACTCAACATTCTCTCAGAACAAGATTCTTGGGCCTTGTTTGTGAGTAAAGCAGGAAGGTCTTTTGAGTCGTCCACTTTCGACGGTGTGGCAAAGAAGGTTTCTGGAGAATGTAGAGGTCTACCGATTGCATTGGTCACAGTTGCAAGGGCGCTCGGAGATAAAGACTTGCTGGAATGGAAGAGAGCAGCTCTTCAGCTAAAGAAGTCGCAAACTGCCAACCCTGACGATTATGGAGAAGCAACAAAATGCATAAAATTAAGCTATGATTACTTGAAAAATGAGGACTACAAGTCATACTTCTTACTTTGTTGCTTATTCCCAGAAGACTTTGACATCCTAACAAAAGACTTGTTCAGGTACGCGATCAGGACAAGATTGTTTCGAGATGCCGAAACACTCCTGGAGGCAAGAGAGCAAGCTGATTCCGTTGTCAAGCATCTTAAATATTCTTGCTTGCTTTTGGATAGTGAAGAAGATGGATGTGTAAGGATGCATGATGTTGTCCGGGATGCAGCCATTCAAATTGTGCAATCTGAAGATGAGTTTTTTGTGAAAGCCGGCTGTCGTTTAGAGGATTGGCCACGTAAATTACATAAAGGCTACTTTGCAATCTCGCTAATGAGGAATTTTATTAGCAAGCTTCCCAAAAATCTGGTATGTCCAAAACTCCAGATTTTGTTACTAAACTGTAATTTCTCTTTAGAAAATATACCAGAAACATTCTTCCAATGTCCAAATGAATTAAGGGTATTAGATCTTAGCTTGACTAGCCTTTCATTACTGCCCAAATCATTCGGTCTCCTAACCAACCTCACAGCTTTGTATTTAGATGATTGTGACTGTCTAAGATTTGACACTTCCATACTGGGAAAACTTAAAAAGCTTGAGATGCTGAGTATGAGAAATTCACGTCTGTATGAGTTGTCAGTAGAAATAGGAGGTTTGACCAATCTGAGGATGTTGGATATTACTGGTAAACATATTTTTAGAATTCCCTCCAAACTGATATCAAAGTTGCATGATTTAGAAGAACTGTACATGCAAGGATTTTGGGCGTGGGAGCGCTTGGAGCCTAACGCTGAGGGAGAAGAAGGAGAAGTCAATGATACCTTTACTAATGCTAGCTTTGACGAGGTAACTAGCTTGTCAAAATTAAGAGTTTTGGAGGTTACTATCAAGGGTGATGACTATATCCCTAAATATGTTGAGTTGAAAATGAATTTGGTTGAGTTTGATGTATGTATTGGTGGTTTCTACGAGCCATACAAGATGAAGTTACATGATCGACATCGTCATATCTCAATGCGCTCGCGTGTCATGATCAGTCACTTCCCGGAGTGGTTTCGCGACGAGATAATGAAGAAAGCAGAGACGCTTCGCCACTCTTGGTGCCAAAGGTTAATCGACATATTTGTGGAATCTGAGCATGGGAGGTTACAGGGACTGAAGCATCTGCATATAGTGGGTCCCAGGAGTCGAGACGTCACGGAGGCGGATCCTGCAATAACATGGGTTCCAAGAAAGCCTGTGTTCGAGAATTTGGAAGAGTTTCATCTGGATAAACTAGATTGTGCAGAGTTGTGTGCCGTTCAATTTCTACCACCTGGGTCTCTATTCAAGTTGAAGGTACTGAAGGTAGAATATTGTTACAACTGGTGGAATATACTTTTACCATCAACATTGTTACATAGACTACCAAATTTGGAAGAATTAACTGTTCGTCGGACGGACAGGAGTGAATATATATTTGGGTGTGAAGCCTTGCTTGTGTTGCAACAATTAAAGTTGAAAAAGATAGAATTGTTTGCTCTAGCAACAGTAAGCATATGTGATGGACCTGCTCCGCGTGCGATGCTCCAGAATCTTCAAAGTTTGTCCATTAAGTGGTGCAAACAGTTGCAGGGAAGTCTCTTCACCTCTGATGTTGCTCAGTGTCTTTCTCAGTTGAAATACTTGGCATTAGAGGGATGCCTTTTGTTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGACCGTCCTTCCAAAATTGAAGCAATTACATTTGAAGGAACTTCCAATGTTATACGAAAGTGCTACTTTTGATATTGAGTGTCCTTCATTGGAGAAAGTGTCTCTGAAGGGCTGTCTTCCACCGGAACAATTGGGATTGAGAAAGATAGCATTGTCCACAGTTAGCATATGTGATGGACCTTCTCGACTACCACGTGCAATGTTCCATAATCTTCAAAGTTTGTTCATAGAGTGGTGCAAGTCAGACGGAAGTCTCTTCACCTATGATGTTGCTCAGTGTCTTTCCCAGCTGAATTCCTTAAAATTAGAGAATTCCCCACTGTTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGACCGTCTTTCCAAAATTGAAGATATTACATTTGGAGGGACTTCCAATGTTGTACTATGAAAGTGCTACTTTTGATATTGTGTGTCCTGAATTGGAAGAGTTCTTTGTGAGTAGCTGCCCCAAGTTTTCAGCCTCTTCATCTGACTTCCACAGCAGGAAACAAGTCCAATTCAGCTGGTGGAAACGCAAAGACTAA

Protein Analysis

1239

Amino Acids

141.79

Weight (kDa)

7.08

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 56 - 220 3.9e-44 TIR domain
TIR_2 PF13676 59 - 153 3.1e-14 TIR domain
NB-ARC PF00931 242 - 405 2e-29 NB-ARC domain
LRR_8 PF13855 607 - 665 5.5e-08 Leucine rich repeat
LRR_14 PF23598 669 - 961 6.6e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 900 - 1043 2.1e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 993 - 1074 2.9e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1110 - 1216 3.1e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 447, 1010
AasI GACNNNNNNGTC 1 cut(s) 758
AatII GACGTC 1 cut(s) 2643
Acc36I ACCTGC 1 cut(s) 3004
AccB1I GGYRCC 1 cut(s) 2547
AccB7I CCANNNNNTGG 2 cut(s) 45, 3042
AccI GTMKAC 2 cut(s) 777, 1276
AccII CGCG 4 cut(s) 1520, 2470, 2505, 3004
AciI CCGC 3 cut(s) 5, 2651, 3002
AclWI GGATC 6 cut(s) 23, 57, 285, 449, 2648, 2661
AcoI YGGCCR 1 cut(s) 1736
AcuI CTGAAG 5 cut(s) 1334, 1713, 2624, 2810, 3293
AcvI CACGTG 1 cut(s) 3362
AcyI GRCGYC 1 cut(s) 2640
AfaI GTAC 7 cut(s) 156, 666, 1101, 1517, 2195, 2787, 3575
AfeI AGCGCT 1 cut(s) 2222
AfiI CCNNNNNNNGG 5 cut(s) 45, 2112, 3042, 3136, 3490
AflIII ACRYGT 1 cut(s) 817
AhdI GACNNNNNGTC 1 cut(s) 1447
AjiI CACGTC 1 cut(s) 2069
AjnI CCWGG 4 cut(s) 38, 1557, 2627, 2761
AjuI GAANNNNNNNTTGG 6 cut(s) 89, 121, 2363, 2395, 2889, 2921
Alw21I GWGCWC 2 cut(s) 625, 829
Alw26I GTCTC 9 cut(s) 520, 941, 1961, 2522, 2631, 2772, 3071, 3273, 3425
Alw44I GTGCAC 1 cut(s) 825
AlwI GGATC 6 cut(s) 23, 57, 285, 449, 2648, 2661
AlwNI CAGNNNCTG 1 cut(s) 2602
Aor51HI AGCGCT 1 cut(s) 2222
AoxI GGCC 2 cut(s) 1188, 1736
ApaLI GTGCAC 1 cut(s) 825
ApeKI GCWGC 7 cut(s) 317, 560, 922, 965, 1343, 1670, 3636
Asp700I GAANNNNTTC 2 cut(s) 636, 2706
AspLEI GCGC 3 cut(s) 1309, 2223, 2466
AspS9I GGNCC 5 cut(s) 194, 1188, 2624, 2993, 3344
AsuC2I CCSGG 2 cut(s) 1664, 2492
AsuHPI GGTGA 4 cut(s) 556, 2351, 3064, 3418
AsuII TTCGAA 1 cut(s) 933
AsuNHI GCTAGC 1 cut(s) 2279
AvaII GGWCC 4 cut(s) 194, 2624, 2993, 3344
AxyI CCTNAGG 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 829
BalI TGGCCA 1 cut(s) 1738
BamHI GGATCC 1 cut(s) 2653
BanI GGYRCC 1 cut(s) 2547
BbrPI CACGTG 1 cut(s) 3362
BbsI GAAGAC 5 cut(s) 426, 1488, 3173, 3275, 3527
Bbv12I GWGCWC 2 cut(s) 625, 829
BbvCI CCTCAGC 1 cut(s) 2238
BbvI GCAGC 7 cut(s) 329, 547, 934, 977, 1355, 1682, 3623
BccI CCATC 4 cut(s) 1628, 2839, 2984, 3335
BceAI ACGGC 2 cut(s) 796, 2729
BciT130I CCWGG 4 cut(s) 40, 1559, 2629, 2763
BclI TGATCA 2 cut(s) 604, 2478
BcnI CCSGG 2 cut(s) 1664, 2492
BcoDI GTCTC 9 cut(s) 520, 941, 1961, 2522, 2631, 2772, 3071, 3273, 3425
BfaI CTAG 6 cut(s) 969, 1926, 2280, 2298, 2723, 2969
BfoI RGCGCY 2 cut(s) 1310, 2224
BfuAI ACCTGC 1 cut(s) 3004
BglII AGATCT 1 cut(s) 1912
BisI GCNGC 7 cut(s) 318, 561, 923, 966, 1344, 1671, 3637
BlsI GCNGC 7 cut(s) 319, 562, 924, 967, 1345, 1672, 3638
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 6 cut(s) 40, 1559, 1664, 2492, 2629, 2763
Bme18I GGWCC 4 cut(s) 194, 2624, 2993, 3344
BmeRI GACNNNNNGTC 1 cut(s) 1447
BmgBI CACGTC 1 cut(s) 2069
BmgT120I GGNCC 5 cut(s) 194, 1188, 2624, 2993, 3344
BmiI GGNNCC 7 cut(s) 195, 2229, 2549, 2625, 2626, 2655, 2673
BmrFI CCNGG 6 cut(s) 40, 1559, 1664, 2492, 2629, 2763
BmrI ACTGGG 1 cut(s) 2033
BmtI GCTAGC 1 cut(s) 2283
BmuI ACTGGG 1 cut(s) 2033
BoxI GACNNNNGTC 1 cut(s) 3417
BpiI GAAGAC 5 cut(s) 426, 1488, 3173, 3275, 3527
BplI GAGNNNNNCTC 2 cut(s) 1330, 1362
BpmI CTGGAG 5 cut(s) 708, 1281, 1580, 1811, 2999
Bpu10I CCTNAGC 1 cut(s) 2238
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 2063
BpuMI CCSGG 2 cut(s) 1664, 2492
BsaAI YACGTR 2 cut(s) 1742, 3362
BsaBI GATNNNNATC 2 cut(s) 276, 2445
BsaHI GRCGYC 1 cut(s) 2640
BsaI GGTCTC 2 cut(s) 1961, 2772
BsaJI CCNNGG 5 cut(s) 414, 723, 1134, 2627, 2762
BsaWI WCCGGW 2 cut(s) 956, 3289
BsaXI ACNNNNNCTCC 2 cut(s) 3251, 3281
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 2112, 3042, 3136, 3490
Bse118I RCCGGY 1 cut(s) 1715
Bse1I ACTGG 4 cut(s) 691, 2028, 2134, 2816
Bse21I CCTNAGG 1 cut(s) 942
Bse3DI GCAATG 2 cut(s) 24, 3372
Bse8I GATNNNNATC 2 cut(s) 276, 2445
BseBI CCWGG 4 cut(s) 40, 1559, 2629, 2763
BseDI CCNNGG 5 cut(s) 414, 723, 1134, 2627, 2762
BseJI GATNNNNATC 2 cut(s) 276, 2445
BseLI CCNNNNNNNGG 5 cut(s) 45, 2112, 3042, 3136, 3490
BseMI GCAATG 2 cut(s) 24, 3372
BseNI ACTGG 4 cut(s) 691, 2028, 2134, 2816
BseSI GKGCMC 1 cut(s) 829
BseXI GCAGC 7 cut(s) 329, 547, 934, 977, 1355, 1682, 3623
BseYI CCCAGC 2 cut(s) 925, 3453
BsgI GTGCAG 1 cut(s) 2751
Bsh1236I CGCG 4 cut(s) 1520, 2470, 2505, 3004
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 1190, 1738
BshNI GGYRCC 1 cut(s) 2547
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 625, 829
BsiSI CCGG 5 cut(s) 957, 1663, 1716, 2492, 3290
BslFI GGGAC 4 cut(s) 862, 2610, 2613, 3573
BslI CCNNNNNNNGG 5 cut(s) 45, 2112, 3042, 3136, 3490
BsmAI GTCTC 9 cut(s) 520, 941, 1961, 2522, 2631, 2772, 3071, 3273, 3425
BsmBI CGTCTC 2 cut(s) 2522, 2631
BsmFI GGGAC 4 cut(s) 862, 2610, 2613, 3573
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 2 cut(s) 1190, 1738
Bso31I GGTCTC 2 cut(s) 1961, 2772
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 625, 829
Bsp1407I TGTACA 1 cut(s) 2193
Bsp19I CCATGG 3 cut(s) 414, 723, 1134
Bsp68I TCGCGA 1 cut(s) 2505
BspACI CCGC 3 cut(s) 5, 2651, 3002
BspANI GGCC 2 cut(s) 1190, 1738
BspFNI CGCG 4 cut(s) 1520, 2470, 2505, 3004
BspHI TCATGA 1 cut(s) 2475
BspLI GGNNCC 7 cut(s) 195, 2229, 2549, 2625, 2626, 2655, 2673
BspMI ACCTGC 1 cut(s) 3004
BspOI GCTAGC 1 cut(s) 2283
BspPI GGATC 6 cut(s) 23, 57, 285, 449, 2648, 2661
BspQI GCTCTTC 2 cut(s) 955, 1353
BspT104I TTCGAA 1 cut(s) 933
BspT107I GGYRCC 1 cut(s) 2547
BspTNI GGTCTC 2 cut(s) 1961, 2772
BsrDI GCAATG 2 cut(s) 24, 3372
BsrFI RCCGGY 1 cut(s) 1715
BsrGI TGTACA 1 cut(s) 2193
BsrI ACTGG 4 cut(s) 691, 2028, 2134, 2816
BssAI RCCGGY 1 cut(s) 1715
BssECI CCNNGG 5 cut(s) 414, 723, 1134, 2627, 2762
BssNI GRCGYC 1 cut(s) 2640
BssT1I CCWWGG 3 cut(s) 414, 723, 1134
Bst2UI CCWGG 4 cut(s) 40, 1559, 2629, 2763
Bst6I CTCTTC 9 cut(s) 138, 955, 1331, 1353, 2697, 3074, 3428, 3612, 3661
BstACI GRCGYC 1 cut(s) 2640
BstAUI TGTACA 1 cut(s) 2193
BstBAI YACGTR 2 cut(s) 1742, 3362
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 9 cut(s) 1575, 1613, 1717, 1796, 2281, 2468, 3006, 3157, 3511
BstDSI CCRYGG 3 cut(s) 414, 723, 1134
BstFNI CGCG 4 cut(s) 1520, 2470, 2505, 3004
BstH2I RGCGCY 2 cut(s) 1310, 2224
BstHHI GCGC 3 cut(s) 1309, 2223, 2466
BstMAI GTCTC 9 cut(s) 520, 941, 1961, 2522, 2631, 2772, 3071, 3273, 3425
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 9 cut(s) 707, 789, 1926, 2047, 2227, 2657, 2929, 3005, 3010
BstNI CCWGG 4 cut(s) 40, 1559, 2629, 2763
BstNSI RCATGY 3 cut(s) 515, 821, 2200
BstPAI GACNNNNGTC 1 cut(s) 3417
BstSCI CCNGG 6 cut(s) 38, 1557, 1662, 2490, 2627, 2761
BstSLI GKGCMC 1 cut(s) 829
BstUI CGCG 4 cut(s) 1520, 2470, 2505, 3004
BstV1I GCAGC 7 cut(s) 329, 547, 934, 977, 1355, 1682, 3623
BstV2I GAAGAC 5 cut(s) 426, 1488, 3173, 3275, 3527
BstX2I RGATCY 3 cut(s) 277, 1912, 2653
BstXI CCANNNNNNTGG 1 cut(s) 1810
BstYI RGATCY 3 cut(s) 277, 1912, 2653
Bsu36I CCTNAGG 1 cut(s) 942
BsuRI GGCC 2 cut(s) 1190, 1738
BtgI CCRYGG 3 cut(s) 414, 723, 1134
BtgZI GCGATG 1 cut(s) 3023
BtrI CACGTC 1 cut(s) 2069
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 5 cut(s) 684, 762, 3095, 3449, 3485
BtuMI TCGCGA 1 cut(s) 2505
BveI ACCTGC 1 cut(s) 3004
Cac8I GCNNGC 9 cut(s) 1575, 1613, 1717, 1796, 2281, 2468, 3006, 3157, 3511
CaiI CAGNNNCTG 1 cut(s) 2602
CciI TCATGA 1 cut(s) 2475
CfoI GCGC 3 cut(s) 1309, 2223, 2466
Cfr10I RCCGGY 1 cut(s) 1715
Cfr13I GGNCC 5 cut(s) 194, 1188, 2624, 2993, 3344
CseI GACGC 2 cut(s) 50, 2539
Csp6I GTAC 7 cut(s) 155, 665, 1100, 1516, 2194, 2786, 3574
CspCI CAANNNNNGTGG 2 cut(s) 792, 827
CviQI GTAC 7 cut(s) 155, 665, 1100, 1516, 2194, 2786, 3574
DraI TTTAAA 1 cut(s) 748
DrdI GACNNNNNNGTC 1 cut(s) 758
DriI GACNNNNNGTC 1 cut(s) 1447
DseDI GACNNNNNNGTC 1 cut(s) 758
EaeI YGGCCR 1 cut(s) 1736
Eam1104I CTCTTC 9 cut(s) 138, 955, 1331, 1353, 2697, 3074, 3428, 3612, 3661
Eam1105I GACNNNNNGTC 1 cut(s) 1447
EarI CTCTTC 9 cut(s) 138, 955, 1331, 1353, 2697, 3074, 3428, 3612, 3661
EciI GGCGGA 2 cut(s) 20, 2666
Eco130I CCWWGG 3 cut(s) 414, 723, 1134
Eco31I GGTCTC 2 cut(s) 1961, 2772
Eco32I GATATC 2 cut(s) 473, 2166
Eco47I GGWCC 4 cut(s) 194, 2624, 2993, 3344
Eco47III AGCGCT 1 cut(s) 2222
Eco57I CTGAAG 5 cut(s) 1334, 1713, 2624, 2810, 3293
Eco72I CACGTG 1 cut(s) 3362
Eco81I CCTNAGG 1 cut(s) 942
EcoO109I RGGNCCY 2 cut(s) 194, 2624
EcoRI GAATTC 4 cut(s) 1056, 2148, 3460, 3478
EcoRII CCWGG 4 cut(s) 38, 1557, 2627, 2761
EcoRV GATATC 2 cut(s) 473, 2166
EcoT14I CCWWGG 3 cut(s) 414, 723, 1134
EcoT22I ATGCAT 2 cut(s) 1409, 1653
ErhI CCWWGG 3 cut(s) 414, 723, 1134
Esp3I CGTCTC 2 cut(s) 2522, 2631
FaqI GGGAC 4 cut(s) 862, 2610, 2613, 3573
FauNDI CATATG 2 cut(s) 2984, 3335
FbaI TGATCA 2 cut(s) 604, 2478
FblI GTMKAC 2 cut(s) 777, 1276
Fnu4HI GCNGC 7 cut(s) 318, 561, 923, 966, 1344, 1671, 3637
Fsp4HI GCNGC 7 cut(s) 318, 561, 923, 966, 1344, 1671, 3637
FspBI CTAG 6 cut(s) 969, 1926, 2280, 2298, 2723, 2969
GlaI GCGC 3 cut(s) 1308, 2222, 2465
GluI GCNGC 7 cut(s) 318, 561, 923, 966, 1344, 1671, 3637
GsaI CCCAGC 2 cut(s) 929, 3457
GsuI CTGGAG 5 cut(s) 708, 1281, 1580, 1811, 2999
HaeII RGCGCY 2 cut(s) 1310, 2224
HaeIII GGCC 2 cut(s) 1190, 1738
HapII CCGG 5 cut(s) 957, 1663, 1716, 2492, 3290
HgaI GACGC 2 cut(s) 50, 2539
HhaI GCGC 3 cut(s) 1309, 2223, 2466
Hin1I GRCGYC 1 cut(s) 2640
Hin6I GCGC 3 cut(s) 1307, 2221, 2464
HinP1I GCGC 3 cut(s) 1307, 2221, 2464
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 3 cut(s) 498, 1796, 2039
HpaII CCGG 5 cut(s) 957, 1663, 1716, 2492, 3290
HphI GGTGA 4 cut(s) 556, 2351, 3064, 3418
Hpy166II GTNNAC 8 cut(s) 112, 684, 778, 827, 1230, 1277, 2135, 3324
Hpy8I GTNNAC 8 cut(s) 112, 684, 778, 827, 1230, 1277, 2135, 3324
Hpy99I CGWCG 4 cut(s) 66, 1241, 2510, 2883
HpyCH4IV ACGT 4 cut(s) 1741, 2068, 2640, 3361
HpyF10VI GCNNNNNNNGC 9 cut(s) 707, 789, 1926, 2047, 2227, 2657, 2929, 3005, 3010
HpySE526I ACGT 4 cut(s) 1741, 2068, 2640, 3361
Hsp92I GRCGYC 1 cut(s) 2640
HspAI GCGC 3 cut(s) 1307, 2221, 2464
KflI GGGWCCC 1 cut(s) 2624
KroI GCCGGC 1 cut(s) 1715
KroNI GCCGGC 1 cut(s) 1717
Ksp22I TGATCA 2 cut(s) 604, 2478
LguI GCTCTTC 2 cut(s) 955, 1353
LmnI GCTCC 4 cut(s) 2218, 2227, 3004, 3018
Lsp1109I GCAGC 7 cut(s) 329, 547, 934, 977, 1355, 1682, 3623
MaeI CTAG 6 cut(s) 969, 1926, 2280, 2298, 2723, 2969
MaeII ACGT 4 cut(s) 1741, 2068, 2640, 3361
MfeI CAATTG 3 cut(s) 342, 675, 3296
MflI RGATCY 3 cut(s) 277, 1912, 2653
MhlI GDGCHC 2 cut(s) 625, 829
MlsI TGGCCA 1 cut(s) 1738
MluNI TGGCCA 1 cut(s) 1738
MlyI GAGTC 3 cut(s) 689, 1232, 2641
MmeI TCCRAC 4 cut(s) 2100, 2861, 3117, 3471
Mox20I TGGCCA 1 cut(s) 1738
Mph1103I ATGCAT 2 cut(s) 1409, 1653
MroNI GCCGGC 1 cut(s) 1715
MroXI GAANNNNTTC 2 cut(s) 636, 2706
MscI TGGCCA 1 cut(s) 1738
MslI CAYNNNNRTG 2 cut(s) 822, 2570
Msp20I TGGCCA 1 cut(s) 1738
MspA1I CMGCKG 3 cut(s) 925, 3457, 3696
MspI CCGG 5 cut(s) 957, 1663, 1716, 2492, 3290
MspR9I CCNGG 6 cut(s) 40, 1559, 1664, 2492, 2629, 2763
MunI CAATTG 3 cut(s) 342, 675, 3296
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 4 cut(s) 40, 1559, 2629, 2763
MvnI CGCG 4 cut(s) 1520, 2470, 2505, 3004
MwoI GCNNNNNNNGC 9 cut(s) 707, 789, 1926, 2047, 2227, 2657, 2929, 3005, 3010
NaeI GCCGGC 1 cut(s) 1717
NciI CCSGG 2 cut(s) 1664, 2492
NcoI CCATGG 3 cut(s) 414, 723, 1134
NdeI CATATG 2 cut(s) 2984, 3335
NgoMIV GCCGGC 1 cut(s) 1715
NheI GCTAGC 1 cut(s) 2279
NlaIV GGNNCC 7 cut(s) 195, 2229, 2549, 2625, 2626, 2655, 2673
NmuCI GTSAC 6 cut(s) 760, 859, 1291, 1994, 2483, 2641
NruI TCGCGA 1 cut(s) 2505
NsiI ATGCAT 2 cut(s) 1409, 1653
NspI RCATGY 3 cut(s) 515, 821, 2200
NspV TTCGAA 1 cut(s) 933
PagI TCATGA 1 cut(s) 2475
PciI ACATGT 1 cut(s) 817
PciSI GCTCTTC 2 cut(s) 955, 1353
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 3358
PctI GAATGC 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 1717
PdmI GAANNNNTTC 2 cut(s) 636, 2706
PfeI GAWTC 7 cut(s) 97, 329, 883, 1181, 1580, 2576, 3019
PflFI GACNNNGTC 1 cut(s) 2448
PflMI CCANNNNNTGG 2 cut(s) 45, 3042
PfoI TCCNGGA 3 cut(s) 1557, 1662, 2490
PkrI GCNGC 7 cut(s) 319, 562, 924, 967, 1345, 1672, 3638
Ple19I CGATCG 1 cut(s) 235
PleI GAGTC 3 cut(s) 688, 1231, 2640
PmaCI CACGTG 1 cut(s) 3362
PmlI CACGTG 1 cut(s) 3362
PpsI GAGTC 3 cut(s) 688, 1231, 2640
Ppu21I YACGTR 2 cut(s) 1742, 3362
PpuMI RGGWCCY 2 cut(s) 194, 2624
PscI ACATGT 1 cut(s) 817
PshAI GACNNNNGTC 1 cut(s) 3417
PsiI TTATAA 2 cut(s) 447, 1010
Psp5II RGGWCCY 2 cut(s) 194, 2624
Psp6I CCWGG 4 cut(s) 38, 1557, 2627, 2761
PspCI CACGTG 1 cut(s) 3362
PspFI CCCAGC 2 cut(s) 925, 3453
PspGI CCWGG 4 cut(s) 38, 1557, 2627, 2761
PspN4I GGNNCC 7 cut(s) 195, 2229, 2549, 2625, 2626, 2655, 2673
PspPI GGNCC 5 cut(s) 194, 1188, 2624, 2993, 3344
PspPPI RGGWCCY 2 cut(s) 194, 2624
PstNI CAGNNNCTG 1 cut(s) 2602
PsuI RGATCY 3 cut(s) 277, 1912, 2653
PsyI GACNNNGTC 1 cut(s) 2448
PvuI CGATCG 1 cut(s) 235
PvuII CAGCTG 3 cut(s) 925, 3457, 3696
RruI TCGCGA 1 cut(s) 2505
RsaI GTAC 7 cut(s) 156, 666, 1101, 1517, 2195, 2787, 3575
RsaNI GTAC 7 cut(s) 155, 665, 1100, 1516, 2194, 2786, 3574
RseI CAYNNNNRTG 2 cut(s) 822, 2570
SapI GCTCTTC 2 cut(s) 955, 1353
SatI GCNGC 7 cut(s) 318, 561, 923, 966, 1344, 1671, 3637
Sau96I GGNCC 5 cut(s) 194, 1188, 2624, 2993, 3344
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 3 cut(s) 689, 1232, 2641
ScrFI CCNGG 6 cut(s) 40, 1559, 1664, 2492, 2629, 2763
SduI GDGCHC 2 cut(s) 625, 829
SfuI TTCGAA 1 cut(s) 933
SinI GGWCC 4 cut(s) 194, 2624, 2993, 3344
SmiMI CAYNNNNRTG 2 cut(s) 822, 2570
SmlI CTYRAG 1 cut(s) 2042
SmoI CTYRAG 1 cut(s) 2042
SsiI CCGC 3 cut(s) 5, 2651, 3002
SspI AATATT 2 cut(s) 1604, 2801
SspMI CTAG 6 cut(s) 969, 1926, 2280, 2298, 2723, 2969
StyD4I CCNGG 6 cut(s) 38, 1557, 1662, 2490, 2627, 2761
StyI CCWWGG 3 cut(s) 414, 723, 1134
TaiI ACGT 4 cut(s) 1744, 2071, 2643, 3364
TaqII GACCGA 1 cut(s) 1943
TatI WGTACW 3 cut(s) 154, 2193, 3573
TfiI GAWTC 7 cut(s) 97, 329, 883, 1181, 1580, 2576, 3019
TscAI CASTG 5 cut(s) 691, 769, 3095, 3449, 3492
TseFI GTSAC 6 cut(s) 760, 859, 1291, 1994, 2483, 2641
TseI GCWGC 7 cut(s) 317, 560, 922, 965, 1343, 1670, 3636
Tsp45I GTSAC 6 cut(s) 760, 859, 1291, 1994, 2483, 2641
TspGWI ACGGA 5 cut(s) 1052, 1573, 2660, 2900, 3429
TspRI CASTG 5 cut(s) 691, 769, 3095, 3449, 3492
Tth111I GACNNNGTC 1 cut(s) 2448
Van91I CCANNNNNTGG 2 cut(s) 45, 3042
VneI GTGCAC 1 cut(s) 825
VpaK11BI GGWCC 4 cut(s) 194, 2624, 2993, 3344
XceI RCATGY 3 cut(s) 515, 821, 2200
XmiI GTMKAC 2 cut(s) 777, 1276
XmnI GAANNNNTTC 2 cut(s) 636, 2706
XspI CTAG 6 cut(s) 969, 1926, 2280, 2298, 2723, 2969
ZraI GACGTC 1 cut(s) 2641
ZrmI AGTACT 1 cut(s) 156
Zsp2I ATGCAT 2 cut(s) 1409, 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.