FvH4_3g43630

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018015
Physical Location & Seq
Reverse (-)
36338028 .. 36346160
8133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43630.t1

Sequence Viewer

Length: 6177 bp
ATGGCGGAGGCGAGGATCGGTTGCATAGCCATTATTAAGTGGATATGGACTCAAGTGAAGCGTCGGTTCAGTTACATGGTCGAAAATTCCCAGGATAATCAAGTATTGGTGGACATGGATGCTTCTGCATCTCTTCCTTCTTCATCATCACCTGTTGAATGGAAGTATGATGTTTTTTTGAGTTTCAGGGGTTCTGACACTCGCAAGGGGATCACAGTCGACATATACGATGGACTCAATCGGGGAGGCATTAAAGCATTCATGGATGATCCAGATCTTGTAGTCGGAGATTCCATTTCTCCCACTCTCATAGCAGCAATTAAAGGGTCAAGGTATGCAATTGTTGTTCTCTCCCAAAATTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTGTCCATGGAAGACAACAGAATTTTGCCACTTTTTTATCATGTTGATCCTACTGATGTTCGATATCAGAAGAAGGGAAGTTTCAAAGAAGCTTTCTATAAGCATGAAACCTCTGGGCAGCACAGAATGGAAAAGGTGAAGGAGTGGAAAGCTGCCTTGAACAAAGTAGCCAATATCTCTGGGTGGAATACAACTGATCATAAAACTGAGAGAGCACTTACCGAAGCCATTGTGAAACTTCTCAGCAGTAAAATTGTACCTGATGCAATTGAGTCCACTAGAGATTTCCAAGCATTTGAAGCAACAAGAATAGCCATGGATGAGGTTATGAAGGCCCTTAAAGATGACGAGGTCACTGCTGTTGGTGTCTACGGCATGGGAGGTGTGGGCAAGACAACCTTGGTGGAACATGTTGGCGCACATGCCCGGAAAAGTGGAATTTTTGATCATGTGATTATGGGTGTCCTATCCCAAACGCCTGACTTGGCAAAAATTCAAGGCACATTGTCAGAACTATTGAGCTTCAAATTTGAGGAGGATACAGAAATTGGAAGAGCAGCTAGATTGAGTAAGGAGATAAGGAGAAGACAAAGGATTCTTATAATCCTGGACGACATTTGGGAGAGAACAGAATTATCAAGAATAGGAATTCCAAGCTACAAGGAACTTCAAAATTGCAATTCCAAAATCCTACTCACCACAAGGATAAGGAATGTTTGTCATGCCATGGAGTGCCAAAGAAAGATCATCCTCAACGTTCTCTCAGAACAAGATTCTTGGGTCTTGTTTGTGAGAAAAGCAAGAAGGTATTTTGAATCCCCCACTTTCGAGGGTGTGGCAAAGAAGGTAGCCGGAGAATGTAGGGGCTTACCGATTGCATTGATAACAGTTGCAAGGGCACTCGGAGACAAAGACTTGGTGGATTGGAGGAGAGCAGCTCAGCGACTAGAGAAGTCGCAAACTGCCAACCCTGATGATAAGGGAGAAGCATCTAAATGTATAAAATTAAGCTATGATTACTTGGAAGATGAGGACTACAGGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTATGACATCCCTATAGAAGACTTGTTCAGGTATGGGATCAGGACAAGATTGTTTCGAGATGCCGAAACACTCATGGAGGCAAGAGAGCAAGCTGATTCCGTTATCAAGCATCTTAAATATTCTTGCTTGCTTTTGGATAGTGAAGAAGATAGATGTGTAAGGATGCATGATGTTGTCCGGGATGCAGCCATTCAAATTGTGCAATCTGAAGATGAGTTTTTTGTGAAAGCCGGCTGTGGTTTAGAGGATTTGCCACGTAAATTACATAAAGGCTACCTTGCAATCTCACTAATGAGGAATTATATTAGCAAGCTTCCCAAAAATCTGGTATGTCCAAAACTCCAGATCTTGTTACTAAACCGTAATTTCTCTTTAGAAAACATACCAGAAACATTCTTCCAATGTCCAAATGAATTAAGGGTATTAGATCTTAGCTTGACTAGCCTTTCATTACTGCCCAAATCATTCGGTGTCCTAACCAACCTCACAGCTTTGTATTTAGATGATTGTACCCATCTAAGAATTGACACTTCCATACTGGGAAAACTTAAAAAGCTTGAGATTCTGAGTATGAGAAATTCACGTCTGTATGAGTTGTCGGTAGAAATAGGAGGTTTGACCAATCTGAGGATGTTGGATATTACTGGTGAACATATTTCTAGAATTCCTTCCAAACTGATATCAAAGTTGCATGATTTAGAAGAACTGTATATGCAAGGATTTTGGGAGTGGGAACGCTTGTGGCCTTACAGACGAGGGGAAGTCAATGATACCTTTACTAATGCTAGCTTTGACGAGGTAACTAGCTTGTCAAAATTAAGAGTTTTGAAGGTTACTATCATGGGTGATGAATATATCCCTAAATATGTTGAGTTGAAAATGAATTTGGTTGAGTTTGATATATGTATTGGTGGTTCATATGAGCCTGGTTTATACGAGCCATACAAGACGAAGTTATATGATCGACATCGTCATATCTCAATACTCTTGTTTGTCATGATCAGTCACTTACCGGAGTGGTTTCGTGACGAGATAATAGAGAATGCGGAGACGCTTTACCACTCTTGGTGCCAAAGGTTAATCGACATATTTGTGGAATCTGAGAATGGGAGGTTACAGAGACTGAAGCATCTGCATATAGTGGGTTCCTACGATCAAGACATCACGGAGGTGGATCCTGCAATAACATGGGTTCCAAAAAAGCCTGTGTTCGAGAATTTGGAAGAGTTGTATCTGGCTGAACTGGATTGTGCAGAGTTGTGTGCCGTTCAATTTCTACCACCTGGATCTCTATTCAAGTTAAAGGTATTGAAAGTAAAAGATTGTTACAACTGGTGGAATATGATTTTACCATCAACATTGTTACATAGACTACCAAATTTGGAAAAATTAATTGTTCATCGGACGGACGGGAGTGAATTTATGTTTGGGTATGAAGCCTTGCTTGTGTTGCGACAATTAAAGTTGAAAAAGATAAAATTGTATGATCTAGCAACAGTAAGCATATGTGAGGGACCTGCTCCTCCTGCGATGCTCCAGAATCTTCGAAGTTTGTCCATTCATCGTTGCAAGCAGTTGCGGGGAAGTCTCTTCACCTCTGATGTTGCTCAGTGTCTTTCTCAGTTGAATTCCTTAGAATTAGAGAGATGCCCCTTGTTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGACCGTCCTTCCAGAATTGAAGCGATTACTTTTGGTGGAACTTCCATTGTTGTACTATGAAAGTGCTACTTTTGATATCGTGTGTCCTGAATTGGAAGAGTTCTTTGTGAGTAGCTGCCCCAAGTTTTCAGCCTCTTCATCTGACTTCCACAGCAGGAAACAAGTCCAATTCAGCTGGACTCACAAACAACTTGTTGACCACATTGTGGAAACTCTCCGCAAGAAAGTACCACCGGATGCAATTGAATCCACAGATGATTTCCAATCATATGAAGCAACAACAAAAGCCATGGATGAGGTTAAGGAGGCGCTAAGAGATGATGAGATCACTGCCGTTGGTGTCTACGGCATGGGCGGCGTTGGAAAGACAACCATGGTGACACATGTAGCTGCACAAGCCTGCAGAAATGGCACTTTTAACCATTGGATTGTAGCTTCCATATCCCAAAGCCCCGACTTCACACAAATCCAAGACACATTAGCAGATCTGCTAGGCTTCAAATTAAAAGAGAAAACAGAAGCTGGAAGAGCTGCTAGGTTGCATAAGGAGATCATGAGAAGAGAAAAACTCCTTATAATCTTGGACGATGTATGGGATATAATAGAGTTGTCCAAGATAGGGATCCCCAACTATAAAGAGCTTCAAACTGTCAATTCCAAAGTCCTACTAACAACAAGGAGAAGGAATGTCTGTCATGTTATGGATTGCGAAAAAAAGATCACCCTCAACATTCTATCAAAAGAAGATTCTTGGGCGTTGTTTGTGAGAAAAGCAAGAAAGTCTTTTGAACAACGAAAGTTCGTGGATGTGGCAAAGAAGGTAGCTGGAGAATGTGGGGGTCTACCGATTGCATTGATAACAGTTGCAAGGGCACTCGGAGATAAAGAGTTGGTGGAATGGGAGAGAGCAGCTAAACGACTAGAGAAGTCACAAACTGCTAATCCTGACGATAACCGAGAAGCATCCCAATGTATAAAATTAAGCTATGATTACTTGAAAGATAAGGAAAAGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTCTTTTATCCCAACAGAAAAACTGTTCATGTATGCGATTGGGAAAGGATTGTTTAGAGATGCTGAGACACTCGAAGAAGCCAGAGAAAACGCTAGTTCAGTGGCCAACCACCTTATACATTCTTGCTTGCTGTTGGAGAGTAGTGATGATACATTTGTAAGGATTCATGATGTCGTCCGGGATACTGCCCTTCAAATTGCGGAATCTGAAGATGGGCATGGGTTTTTGGTGAAAGCTGGCTCTCGTTTAAAGGATTGGCCACGTCAAGTACATGAAGGCTACACTGCAATCTCACTAATGAGGAGTCCTATGAGCAAGCTACCCGAGAATCTGGTATGTCCAAAACTCGAGATTTTGTTACTGAACGGTAATTCCAGTTTAAATGAGATCCCAGAAACCTTTTTTCAAAGTCCATATGAATTAAGGGTGTTAGATCTTAGCTGGACAAGTATTTCATCACTACCCCAATCCTTCAGTCTCCTAACCAACCTCCAAGCTTTGTATTTAGACAACTGTCATGGTCTCAATCTAATAACTGACTATTCCAGCCTAGGAAACCTTGAAAAGCTTGAGATTCTGAGTATAAGAAAGTCAATTGTGAAGGAGTGGTCGATAGAAATAGGAGGTTTGACCAATCTAAGGATGTTGGATCACAACGGTGGACTTATGGCTAGAATTCCATCCAAGTTGATATCAAAGTTGCATAATTTAGAAGAACTGTACTTGCCAGGATTTTGGTTGTGGGGGGTTAAAGTTAAGGGAGAAGGAGAAGAAACCAATGCAGGCTTTGACGAGGTAACTAGCTTGACAAAATTAAGAGTTTTGAAGGTTAGTATGTTAGATGTAGAATGTATCCCCAAGTATGTTGAGTCCATACCGGATTGGGTTGAGTTTCATATACGTATAGGTAAATACGACCCAATCAAGATTCCATCCCATGATGAGTATGATCGATACGGTCATATTAACTCAATATGCTTGAAGCTTGACATCATCAGTCGCTTACCGGATTGGTTTCGCAACGAGATAATGAAGAAAACAGAGCATCTAGTATGGGAAGAGGGCCGAAGGTTAATTGACATATTTGTGGAATCTGAGCATGGGAGATTAGAGGAGCTGAAGCAGCTTGAAATAGTGGGTCCCGAGAGTATCATGGAGCCGACGAACACAATAACATGGGTTCCAGAGAAACCTGTGTTCGAGAATTTGGAAGCATTGCATCTGCTTGAGCTGGATTGTGCGGAGTTATGTGCTCTTGAAGTTCTACCACCTGGGTCTTTTTTCAATTTGAAGTTATTGAAGGTAGAAACTTGTTACAACTGGGGGAATATACTTTTACCATCTACATTGTTACAGAGACTGCTAAATTTGGAAGAACTATATTGTAAAACAATGTACCAGAGTGAATATGTGTTTGGGTTTGAAGAAGCGTCACATATTGTGTCAAAACAATTAAAACTGAGAAAGATAGAATTGTCTGATACGGATATAGTAAGCCTATGTGATGGACCTGCTCCACCTGCAATGTTCCAGAATCTTCAAAGTTTCTCCATTACAAACTGCATGTTGCTGGGAAGTCTCTTCACATATGATGTTGCTCAATGTCTTTCTCAGCTGAATTGCTTGGTATTAGAGGGATGCCTCTATTTGGAAAAGATTGTTGAAGCAAACAACGAGAAGATCATCCTTCCAAAATTGGAGAAATTAAGTTTGATAGAACTTCCAATGTTGGACTATGAAAGTGCTACTTTTGATATTCAGTGTCCTTCATTGGAATACTTGGAATTGTGTGACTGCCCCAAGTTTTCAGTCTCTTCCTCTGACTTCCACAGCTGGAAACAAGTCCAAGTCAAACTCAATAGGGAGGCCTGTGGCCTCAGAATGCCTAGTATATGGAGGGAGCCAGTTGCATTTTGTGTGGAGGAAGCAAAGGGAAGAAAACGAGTTGGTAATGAATGA

Protein Analysis

2059

Amino Acids

235.49

Weight (kDa)

5.71

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 55 - 220 1.2e-43 TIR domain
TIR_2 PF13676 58 - 152 2.7e-13 TIR domain
NB-ARC PF00931 241 - 402 3.7e-29 NB-ARC domain
LRR_8 PF13855 606 - 664 8.4e-07 Leucine rich repeat
LRR_14 PF23598 632 - 730 5e-08 Leucine-rich repeat region
LRR_14 PF23598 659 - 794 1.9e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 901 - 1044 5.7e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 984 - 1098 7.3e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
NB-ARC PF00931 1151 - 1315 2.1e-31 NB-ARC domain
LRR_8 PF13855 1520 - 1579 2.2e-09 Leucine rich repeat
LRR_14 PF23598 1546 - 1707 3.3e-12 Leucine-rich repeat region
LRR_14 PF23598 1590 - 1755 2.2e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 1810 - 1953 8.9e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1894 - 2007 4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1007, 3746
AarI CACCTGC 1 cut(s) 5746
Acc36I ACCTGC 3 cut(s) 3007, 5737, 5746
AccB1I GGYRCC 1 cut(s) 2550
AccB7I CCANNNNNTGG 1 cut(s) 3376
AccI GTMKAC 4 cut(s) 219, 774, 3513, 4012
AciI CCGC 7 cut(s) 5, 2528, 3061, 3388, 3525, 4418, 5459
AclI AACGTT 1 cut(s) 1161
AcoI YGGCCR 2 cut(s) 4320, 4475
AcuI CTGAAG 5 cut(s) 1710, 2627, 4446, 4675, 5357
AdeI CACNNNGTG 1 cut(s) 3376
AfaI GTAC 7 cut(s) 663, 1993, 3224, 3399, 4488, 4940, 5615
AfiI CCNNNNNNNGG 6 cut(s) 2109, 3139, 3376, 3789, 4857, 5866
AflIII ACRYGT 2 cut(s) 814, 3553
AhdI GACNNNNNGTC 1 cut(s) 1444
AjiI CACGTC 2 cut(s) 2066, 4481
AjnI CCWGG 6 cut(s) 90, 1011, 2407, 2764, 4945, 5488
AjuI GAANNNNNNNTTGG 6 cut(s) 2351, 2383, 2892, 2924, 5616, 5648
AleI CACNNNNGTG 2 cut(s) 2651, 4875
AloI GAACNNNNNNTCC 2 cut(s) 4574, 4606
Alw21I GWGCWC 2 cut(s) 622, 5473
AlwNI CAGNNNCTG 3 cut(s) 2605, 3692, 5578
Ama87I CYCGRG 3 cut(s) 4541, 4565, 5360
AoxI GGCC 7 cut(s) 738, 2225, 4320, 4475, 5281, 6084, 6091
AseI ATTAAT 1 cut(s) 2873
Asp700I GAANNNNTTC 1 cut(s) 2149
AspA2I CCTAGG 1 cut(s) 4768
AspLEI GCGC 2 cut(s) 824, 3481
AspS9I GGNCC 5 cut(s) 739, 2996, 5281, 5357, 5726
AsuC2I CCSGG 3 cut(s) 832, 1661, 4397
AsuHPI GGTGA 9 cut(s) 141, 553, 1093, 2141, 2339, 3067, 3559, 3883, 4459
AsuII TTCGAA 1 cut(s) 3028
AsuNHI GCTAGC 1 cut(s) 2267
AvaI CYCGRG 3 cut(s) 4541, 4565, 5360
AvaII GGWCC 3 cut(s) 2996, 5357, 5726
AvrII CCTAGG 1 cut(s) 4768
BaeGI GKGCMC 2 cut(s) 1306, 4045
BaeI ACNNNNGTAYC 2 cut(s) 4359, 4392
BalI TGGCCA 2 cut(s) 4322, 4477
BamHI GGATCC 2 cut(s) 2656, 3792
BanI GGYRCC 1 cut(s) 2550
BbsI GAAGAC 6 cut(s) 423, 997, 1485, 1506, 3176, 4221
Bbv12I GWGCWC 2 cut(s) 622, 5473
BccI CCATC 8 cut(s) 224, 2004, 2842, 4424, 4906, 5158, 5566, 5717
BceAI ACGGC 4 cut(s) 793, 2732, 3488, 3532
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 6 cut(s) 92, 1013, 2409, 2766, 4947, 5490
BciVI GTATCC 3 cut(s) 937, 4393, 5081
BclI TGATCA 3 cut(s) 601, 850, 2481
BcnI CCSGG 3 cut(s) 832, 1661, 4397
BfmI CTRYAG 3 cut(s) 1441, 1494, 3571
BfoI RGCGCY 1 cut(s) 3482
BfuAI ACCTGC 3 cut(s) 3007, 5737, 5746
BfuI GTATCC 3 cut(s) 937, 4393, 5081
BglII AGATCT 5 cut(s) 274, 1827, 1909, 3655, 4651
BlnI CCTAGG 1 cut(s) 4768
BlpI GCTNAGC 1 cut(s) 1344
Bme1390I CCNGG 9 cut(s) 92, 832, 1013, 1661, 2409, 2766, 4397, 4947, 5490
Bme18I GGWCC 3 cut(s) 2996, 5357, 5726
BmeRI GACNNNNNGTC 1 cut(s) 1444
BmeT110I CYCGRG 3 cut(s) 4541, 4565, 5360
BmgBI CACGTC 2 cut(s) 2066, 4481
BmgT120I GGNCC 5 cut(s) 739, 2996, 5281, 5357, 5726
BmrFI CCNGG 9 cut(s) 92, 832, 1013, 1661, 2409, 2766, 4397, 4947, 5490
BmrI ACTGGG 2 cut(s) 2030, 5548
BmtI GCTAGC 1 cut(s) 2271
BmuI ACTGGG 2 cut(s) 2030, 5548
BoxI GACNNNNGTC 1 cut(s) 4731
BpiI GAAGAC 6 cut(s) 423, 997, 1485, 1506, 3176, 4221
BplI GAGNNNNNCTC 4 cut(s) 1327, 1359, 3723, 3755
BpmI CTGGAG 3 cut(s) 1808, 3002, 4017
Bpu1102I GCTNAGC 1 cut(s) 1344
Bpu14I TTCGAA 1 cut(s) 3028
BpuEI CTTGAG 4 cut(s) 36, 2060, 4808, 5465
BpuMI CCSGG 3 cut(s) 832, 1661, 4397
Bsa29I ATCGAT 1 cut(s) 5170
BsaAI YACGTR 2 cut(s) 1739, 5120
BsaBI GATNNNNATC 3 cut(s) 273, 2448, 3791
BsaI GGTCTC 1 cut(s) 4745
BsaJI CCNNGG 9 cut(s) 90, 411, 720, 804, 1131, 3459, 3543, 4768, 5489
BsaWI WCCGGW 4 cut(s) 2494, 3403, 5095, 5224
Bsc4I CCNNNNNNNGG 6 cut(s) 2109, 3139, 3376, 3789, 4857, 5866
Bse118I RCCGGY 1 cut(s) 1712
Bse1I ACTGG 7 cut(s) 2025, 2131, 2730, 2819, 4593, 5543, 6122
Bse3DI GCAATG 2 cut(s) 5432, 5748
Bse8I GATNNNNATC 3 cut(s) 273, 2448, 3791
BseBI CCWGG 6 cut(s) 92, 1013, 2409, 2766, 4947, 5490
BseCI ATCGAT 1 cut(s) 5170
BseDI CCNNGG 9 cut(s) 90, 411, 720, 804, 1131, 3459, 3543, 4768, 5489
BseJI GATNNNNATC 3 cut(s) 273, 2448, 3791
BseLI CCNNNNNNNGG 6 cut(s) 2109, 3139, 3376, 3789, 4857, 5866
BseMI GCAATG 2 cut(s) 5432, 5748
BseNI ACTGG 7 cut(s) 2025, 2131, 2730, 2819, 4593, 5543, 6122
BseRI GAGGAG 5 cut(s) 953, 1348, 2994, 4534, 5345
BseSI GKGCMC 2 cut(s) 1306, 4045
BseYI CCCAGC 1 cut(s) 5788
BsgI GTGCAG 2 cut(s) 2754, 3546
BshFI GGCC 7 cut(s) 740, 2227, 4322, 4477, 5283, 6086, 6093
BshNI GGYRCC 1 cut(s) 2550
BshVI ATCGAT 1 cut(s) 5170
BsiHKAI GWGCWC 2 cut(s) 622, 5473
BsiHKCI CYCGRG 3 cut(s) 4541, 4565, 5360
BsiSI CCGG 9 cut(s) 832, 1257, 1660, 1713, 2495, 3404, 4396, 5096, 5225
BslFI GGGAC 2 cut(s) 3009, 5343
BslI CCNNNNNNNGG 6 cut(s) 2109, 3139, 3376, 3789, 4857, 5866
BsmBI CGTCTC 1 cut(s) 2525
BsmFI GGGAC 2 cut(s) 3009, 5343
BsmI GAATGC 3 cut(s) 257, 2530, 6105
BsnI GGCC 7 cut(s) 740, 2227, 4322, 4477, 5283, 6086, 6093
Bso31I GGTCTC 1 cut(s) 4745
BsoBI CYCGRG 3 cut(s) 4541, 4565, 5360
Bsp119I TTCGAA 1 cut(s) 3028
Bsp1286I GDGCHC 4 cut(s) 622, 1306, 4045, 5473
Bsp1720I GCTNAGC 1 cut(s) 1344
Bsp19I CCATGG 5 cut(s) 411, 720, 1131, 3459, 3543
BspACI CCGC 7 cut(s) 5, 2528, 3061, 3388, 3525, 4418, 5459
BspANI GGCC 7 cut(s) 740, 2227, 4322, 4477, 5283, 6086, 6093
BspDI ATCGAT 1 cut(s) 5170
BspHI TCATGA 3 cut(s) 2478, 3723, 4384
BspMAI CTGCAG 1 cut(s) 3575
BspMI ACCTGC 3 cut(s) 3007, 5737, 5746
BspOI GCTAGC 1 cut(s) 2271
BspQI GCTCTTC 2 cut(s) 952, 3691
BspT104I TTCGAA 1 cut(s) 3028
BspT107I GGYRCC 1 cut(s) 2550
BspTNI GGTCTC 1 cut(s) 4745
BsrDI GCAATG 2 cut(s) 5432, 5748
BsrFI RCCGGY 1 cut(s) 1712
BsrI ACTGG 7 cut(s) 2025, 2131, 2730, 2819, 4593, 5543, 6122
BssAI RCCGGY 1 cut(s) 1712
BssECI CCNNGG 9 cut(s) 90, 411, 720, 804, 1131, 3459, 3543, 4768, 5489
BssT1I CCWWGG 7 cut(s) 411, 720, 804, 1131, 3459, 3543, 4768
Bst2UI CCWGG 6 cut(s) 92, 1013, 2409, 2766, 4947, 5490
BstBAI YACGTR 2 cut(s) 1739, 5120
BstBI TTCGAA 1 cut(s) 3028
BstDSI CCRYGG 5 cut(s) 411, 720, 1131, 3459, 3543
BstH2I RGCGCY 1 cut(s) 3482
BstHHI GCGC 2 cut(s) 824, 3481
BstNI CCWGG 6 cut(s) 92, 1013, 2409, 2766, 4947, 5490
BstNSI RCATGY 4 cut(s) 818, 830, 3557, 5785
BstPAI GACNNNNGTC 1 cut(s) 4731
BstSCI CCNGG 9 cut(s) 90, 830, 1011, 1659, 2407, 2764, 4395, 4945, 5488
BstSFI CTRYAG 3 cut(s) 1441, 1494, 3571
BstSLI GKGCMC 2 cut(s) 1306, 4045
BstSNI TACGTA 1 cut(s) 5120
BstV2I GAAGAC 6 cut(s) 423, 997, 1485, 1506, 3176, 4221
BstX2I RGATCY 9 cut(s) 274, 1827, 1909, 2656, 2768, 3655, 3792, 4605, 4651
BstXI CCANNNNNNTGG 2 cut(s) 1807, 4953
BstYI RGATCY 9 cut(s) 274, 1827, 1909, 2656, 2768, 3655, 3792, 4605, 4651
Bsu15I ATCGAT 1 cut(s) 5170
BsuI GTATCC 3 cut(s) 937, 4393, 5081
BsuRI GGCC 7 cut(s) 740, 2227, 4322, 4477, 5283, 6086, 6093
BsuTUI ATCGAT 1 cut(s) 5170
BtgI CCRYGG 5 cut(s) 411, 720, 1131, 3459, 3543
BtgZI GCGATG 1 cut(s) 3026
BtrI CACGTC 2 cut(s) 2066, 4481
BtsI GCAGTG 3 cut(s) 759, 3498, 4500
BtsIMutI CAGTG 6 cut(s) 759, 3098, 3498, 4323, 4500, 5984
BveI ACCTGC 3 cut(s) 3007, 5737, 5746
CaiI CAGNNNCTG 3 cut(s) 2605, 3692, 5578
CciI TCATGA 3 cut(s) 2478, 3723, 4384
CfoI GCGC 2 cut(s) 824, 3481
Cfr10I RCCGGY 1 cut(s) 1712
Cfr13I GGNCC 5 cut(s) 739, 2996, 5281, 5357, 5726
ClaI ATCGAT 1 cut(s) 5170
CseI GACGC 3 cut(s) 50, 2542, 5637
Csp6I GTAC 7 cut(s) 662, 1992, 3223, 3398, 4487, 4939, 5614
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviQI GTAC 7 cut(s) 662, 1992, 3223, 3398, 4487, 4939, 5614
DraI TTTAAA 2 cut(s) 4467, 4599
DraIII CACNNNGTG 1 cut(s) 3376
DriI GACNNNNNGTC 1 cut(s) 1444
EaeI YGGCCR 2 cut(s) 4320, 4475
Eam1105I GACNNNNNGTC 1 cut(s) 1444
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 5120
Eco130I CCWWGG 7 cut(s) 411, 720, 804, 1131, 3459, 3543, 4768
Eco147I AGGCCT 1 cut(s) 6086
Eco31I GGTCTC 1 cut(s) 4745
Eco32I GATATC 4 cut(s) 470, 2163, 3247, 4911
Eco47I GGWCC 3 cut(s) 2996, 5357, 5726
Eco57I CTGAAG 5 cut(s) 1710, 2627, 4446, 4675, 5357
Eco88I CYCGRG 3 cut(s) 4541, 4565, 5360
EcoO109I RGGNCCY 3 cut(s) 739, 2996, 5357
EcoRI GAATTC 4 cut(s) 1053, 2145, 3109, 4893
EcoRII CCWGG 6 cut(s) 90, 1011, 2407, 2764, 4945, 5488
EcoRV GATATC 4 cut(s) 470, 2163, 3247, 4911
EcoT14I CCWWGG 7 cut(s) 411, 720, 804, 1131, 3459, 3543, 4768
EcoT22I ATGCAT 1 cut(s) 1650
ErhI CCWWGG 7 cut(s) 411, 720, 804, 1131, 3459, 3543, 4768
Esp3I CGTCTC 1 cut(s) 2525
FaqI GGGAC 2 cut(s) 3009, 5343
FauI CCCGC 1 cut(s) 3054
FauNDI CATATG 5 cut(s) 2401, 2987, 3439, 4633, 5806
FbaI TGATCA 3 cut(s) 601, 850, 2481
FblI GTMKAC 4 cut(s) 219, 774, 3513, 4012
GlaI GCGC 2 cut(s) 823, 3480
GsaI CCCAGC 1 cut(s) 5792
GsuI CTGGAG 3 cut(s) 1808, 3002, 4017
HaeII RGCGCY 1 cut(s) 3482
HaeIII GGCC 7 cut(s) 740, 2227, 4322, 4477, 5283, 6086, 6093
HapII CCGG 9 cut(s) 832, 1257, 1660, 1713, 2495, 3404, 4396, 5096, 5225
HgaI GACGC 3 cut(s) 50, 2542, 5637
HhaI GCGC 2 cut(s) 824, 3481
Hin6I GCGC 2 cut(s) 822, 3479
HinP1I GCGC 2 cut(s) 822, 3479
HincII GTYRAC 2 cut(s) 220, 3367
HindII GTYRAC 2 cut(s) 220, 3367
HindIII AAGCTT 6 cut(s) 495, 1793, 2036, 4713, 4784, 5201
HpaII CCGG 9 cut(s) 832, 1257, 1660, 1713, 2495, 3404, 4396, 5096, 5225
HphI GGTGA 9 cut(s) 141, 553, 1093, 2141, 2339, 3067, 3559, 3883, 4459
Hpy166II GTNNAC 9 cut(s) 112, 220, 681, 775, 2132, 3367, 3514, 4013, 4880
Hpy8I GTNNAC 9 cut(s) 112, 220, 681, 775, 2132, 3367, 3514, 4013, 4880
Hpy99I CGWCG 2 cut(s) 66, 5383
HpyCH4IV ACGT 5 cut(s) 1161, 1738, 2065, 4480, 5119
HpySE526I ACGT 5 cut(s) 1161, 1738, 2065, 4480, 5119
HspAI GCGC 2 cut(s) 822, 3479
KflI GGGWCCC 1 cut(s) 5357
KroI GCCGGC 1 cut(s) 1712
KroNI GCCGGC 1 cut(s) 1714
Ksp22I TGATCA 3 cut(s) 601, 850, 2481
LguI GCTCTTC 2 cut(s) 952, 3691
LmnI GCTCC 6 cut(s) 3007, 3021, 5332, 5374, 5737, 6118
MaeII ACGT 5 cut(s) 1161, 1738, 2065, 4480, 5119
MfeI CAATTG 4 cut(s) 339, 672, 3411, 4812
MflI RGATCY 9 cut(s) 274, 1827, 1909, 2656, 2768, 3655, 3792, 4605, 4651
MhlI GDGCHC 4 cut(s) 622, 1306, 4045, 5473
MlsI TGGCCA 2 cut(s) 4322, 4477
MluNI TGGCCA 2 cut(s) 4322, 4477
MlyI GAGTC 7 cut(s) 43, 228, 686, 3343, 4211, 4531, 5096
MmeI TCCRAC 7 cut(s) 265, 2097, 3120, 3511, 4332, 4845, 5928
Mox20I TGGCCA 2 cut(s) 4322, 4477
Mph1103I ATGCAT 1 cut(s) 1650
MroNI GCCGGC 1 cut(s) 1712
MroXI GAANNNNTTC 1 cut(s) 2149
MscI TGGCCA 2 cut(s) 4322, 4477
MslI CAYNNNNRTG 6 cut(s) 1399, 2573, 2651, 4138, 4875, 5303
Msp20I TGGCCA 2 cut(s) 4322, 4477
MspA1I CMGCKG 3 cut(s) 3345, 5833, 6051
MspI CCGG 9 cut(s) 832, 1257, 1660, 1713, 2495, 3404, 4396, 5096, 5225
MspR9I CCNGG 9 cut(s) 92, 832, 1013, 1661, 2409, 2766, 4397, 4947, 5490
MunI CAATTG 4 cut(s) 339, 672, 3411, 4812
Mva1269I GAATGC 3 cut(s) 257, 2530, 6105
MvaI CCWGG 6 cut(s) 92, 1013, 2409, 2766, 4947, 5490
NaeI GCCGGC 1 cut(s) 1714
NciI CCSGG 3 cut(s) 832, 1661, 4397
NcoI CCATGG 5 cut(s) 411, 720, 1131, 3459, 3543
NdeI CATATG 5 cut(s) 2401, 2987, 3439, 4633, 5806
NgoMIV GCCGGC 1 cut(s) 1712
NheI GCTAGC 1 cut(s) 2267
NmuCI GTSAC 7 cut(s) 757, 2486, 2507, 3547, 4098, 5649, 6008
NsiI ATGCAT 1 cut(s) 1650
NspI RCATGY 4 cut(s) 818, 830, 3557, 5785
NspV TTCGAA 1 cut(s) 3028
OliI CACNNNNGTG 2 cut(s) 2651, 4875
PaeR7I CTCGAG 1 cut(s) 4565
PagI TCATGA 3 cut(s) 2478, 3723, 4384
PaqCI CACCTGC 1 cut(s) 5746
PceI AGGCCT 1 cut(s) 6086
PciI ACATGT 2 cut(s) 814, 3553
PciSI GCTCTTC 2 cut(s) 952, 3691
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 3 cut(s) 257, 2530, 6105
PdiI GCCGGC 1 cut(s) 1714
PdmI GAANNNNTTC 1 cut(s) 2149
PflFI GACNNNGTC 2 cut(s) 755, 2451
PflMI CCANNNNNTGG 1 cut(s) 3376
PfoI TCCNGGA 3 cut(s) 1011, 1659, 4395
PleI GAGTC 7 cut(s) 43, 228, 685, 3343, 4211, 4530, 5095
PpsI GAGTC 7 cut(s) 43, 228, 685, 3343, 4211, 4530, 5095
Ppu21I YACGTR 2 cut(s) 1739, 5120
PpuMI RGGWCCY 2 cut(s) 2996, 5357
PscI ACATGT 2 cut(s) 814, 3553
PshAI GACNNNNGTC 1 cut(s) 4731
PshBI ATTAAT 1 cut(s) 2873
PsiI TTATAA 2 cut(s) 1007, 3746
Psp1406I AACGTT 1 cut(s) 1161
Psp5II RGGWCCY 2 cut(s) 2996, 5357
Psp6I CCWGG 6 cut(s) 90, 1011, 2407, 2764, 4945, 5488
PspFI CCCAGC 1 cut(s) 5788
PspGI CCWGG 6 cut(s) 90, 1011, 2407, 2764, 4945, 5488
PspPI GGNCC 5 cut(s) 739, 2996, 5281, 5357, 5726
PspPPI RGGWCCY 2 cut(s) 2996, 5357
PsrI GAACNNNNNNTAC 2 cut(s) 2380, 2412
PstI CTGCAG 1 cut(s) 3575
PstNI CAGNNNCTG 3 cut(s) 2605, 3692, 5578
PsuI RGATCY 9 cut(s) 274, 1827, 1909, 2656, 2768, 3655, 3792, 4605, 4651
PsyI GACNNNGTC 2 cut(s) 755, 2451
PvuII CAGCTG 3 cut(s) 3345, 5833, 6051
RsaI GTAC 7 cut(s) 663, 1993, 3224, 3399, 4488, 4940, 5615
RsaNI GTAC 7 cut(s) 662, 1992, 3223, 3398, 4487, 4939, 5614
RseI CAYNNNNRTG 6 cut(s) 1399, 2573, 2651, 4138, 4875, 5303
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 2 cut(s) 952, 3691
Sau96I GGNCC 5 cut(s) 739, 2996, 5281, 5357, 5726
SchI GAGTC 7 cut(s) 43, 228, 686, 3343, 4211, 4531, 5096
ScrFI CCNGG 9 cut(s) 92, 832, 1013, 1661, 2409, 2766, 4397, 4947, 5490
SduI GDGCHC 4 cut(s) 622, 1306, 4045, 5473
SfcI CTRYAG 3 cut(s) 1441, 1494, 3571
Sfr274I CTCGAG 1 cut(s) 4565
SfuI TTCGAA 1 cut(s) 3028
SinI GGWCC 3 cut(s) 2996, 5357, 5726
SlaI CTCGAG 1 cut(s) 4565
SmiMI CAYNNNNRTG 6 cut(s) 1399, 2573, 2651, 4138, 4875, 5303
SmlI CTYRAG 5 cut(s) 51, 2039, 4565, 4787, 5444
SmoI CTYRAG 5 cut(s) 51, 2039, 4565, 4787, 5444
SnaBI TACGTA 1 cut(s) 5120
SseBI AGGCCT 1 cut(s) 6086
SsiI CCGC 7 cut(s) 5, 2528, 3061, 3388, 3525, 4418, 5459
SspI AATATT 1 cut(s) 1601
StuI AGGCCT 1 cut(s) 6086
StyD4I CCNGG 9 cut(s) 90, 830, 1011, 1659, 2407, 2764, 4395, 4945, 5488
StyI CCWWGG 7 cut(s) 411, 720, 804, 1131, 3459, 3543, 4768
TaiI ACGT 5 cut(s) 1164, 1741, 2068, 4483, 5122
TatI WGTACW 3 cut(s) 3222, 4486, 4938
TauI GCSGC 1 cut(s) 3528
TscAI CASTG 6 cut(s) 766, 3098, 3505, 4323, 4507, 5984
TseFI GTSAC 7 cut(s) 757, 2486, 2507, 3547, 4098, 5649, 6008
Tsp45I GTSAC 7 cut(s) 757, 2486, 2507, 3547, 4098, 5649, 6008
TspGWI ACGGA 4 cut(s) 1570, 2663, 2903, 5717
TspRI CASTG 6 cut(s) 766, 3098, 3505, 4323, 4507, 5984
Tth111I GACNNNGTC 2 cut(s) 755, 2451
Van91I CCANNNNNTGG 1 cut(s) 3376
VpaK11BI GGWCC 3 cut(s) 2996, 5357, 5726
VspI ATTAAT 1 cut(s) 2873
XbaI TCTAGA 1 cut(s) 2141
XceI RCATGY 4 cut(s) 818, 830, 3557, 5785
XhoI CTCGAG 1 cut(s) 4565
XmaJI CCTAGG 1 cut(s) 4768
XmiI GTMKAC 4 cut(s) 219, 774, 3513, 4012
XmnI GAANNNNTTC 1 cut(s) 2149
Zsp2I ATGCAT 1 cut(s) 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.