Rw5G047690

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018015
Physical Location & Seq
Reverse (-)
82581119 .. 82597578
16460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047690.1

Sequence Viewer

Length: 6396 bp
ATGGCTGGGCATCTCCTAATTTTAAGCAAATCCCCTCTATTAGAGCTGTTTTGTGACTGGGTAATCTTGAATTGCAGGGGTATAGACACTCGCAAGGGGATCACAGTCGACATACACGATCGGCTAAATGGGAGTGGAATCAAAACATTCATGGATGAGCAGGACCTTCAAGTAGGGGATGCTATTTCTCCCACTCTCTTAGCAGCAATCAAAGAATCAAGGCTTGCAATTGTTGTTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTTTGCCACTTTTCTATCACGTGGAGCCGTCTGATATTCGATATCAGAAGAAGAGGAGTTTCGAAGAAGCTTTCTTTAAGCATGAAACCTCTGGTCGACATGAATTAGAGAAGGTGAAGCAGTGGAGGGATGCTTTATACAAAGTGGCAAATTTCTCTGGGTGGAATACATCTGATTATAAGACTCACAAAAAACTTGTTGACGCCATTGAGGAATTTCTCCGCAGTAAAGTTCTAACTGATGCAATTGGGTCCACAGGAGATTTCAAAACATTTGAAGCAACAAGAGAAGCCATGGATGAGGTTATGAAGGCGCTAAAAGATGACGAGGTCACTACCATTGGTGTCTACGGCATGGGAGGAGTTGGAAAGACAACCATGGTAAGACATGTCGGTGCACAAGCCCAAAAAAAAGGGATTTTTCATCATGTGATTATGGCAGTCATATCCCAAAGCCCTGACTTGAAAAAAATTCAAGGAACATTGGCAGAACTGTTGGGATTCGAATTGAAGAAGAAGACAGAAATTGGAAGAGCAGCTAAATTGAGTATGGAGATAATGAGAAGAACAAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATAGACATATCAAGAATAGGAATACCTAGCTACAAGGAACTTCAAAAGTGCAATTCCAAAGTACTACTCACCACAAGGATAAGGAATGTCTGTCATGTCATGAGATGCCAAAGAAAGATCACCCTCAGTATCCTATCAAAAGATGATTCTTGGACCTTATTTGTGAGAAATGCAGGAAGGTCTTTAGAATCCACCAATTTCGAGGATGTAGCGAGGAGGGTAGCTAGAGAATGCTGTGGTCTACCGATTGCATTGATAACAGTTGCGAGGGCACTCGGAGATAAAGACTTGGTGGAATGGAAAAGAGCAGTTCAACGACTAGAGAAGTCGCAAACTGCCAACCCTGATGATTACGGAGAAGCATCTAAATGTATAAAATTAAGCTATAATTACTTGAAAGATGAGGACTACAAGTCATACTTCTTGCTCTGTTGCTTATTTCCAGAAGACTCTGAAATCGAAATGGAGAGCTTGTTCAGGTATGCGATCGGGAAAGGATTGTTTCGAGATACCGACACACTCGAAGAAGCTAGAGAGAGAGCTGATTCCGTGGCCACGCACCTTAAACATTCTTGCTTGCTTTTGGAGGGAGAAAGATTTAGACACGCAGAAAGAATTAGATGTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAAATTGCGCAATCTGAAGATGGGTTTTTGGTGAAAGCTGGCTGTGATTTAAAGAATTGGCCACGTCAATTACATGAAGGCTACACTGCAGTCTCACTAATGCGGAATTTTATTTGCAAGCTACCCGAAAAGTTGGTATGTCCAAAACTCCAAATTTTGTTGCTAAACTCTAATTTCTCAATAGAAGAGATCCCAGAAACTTTTTTACAAAGTCCCAATGAATTAACGGTCTTAGATCTAAGCCGGACTAGTATTTCGTTACTACCCCGATCATTCAGTCTCCTAACCAACCTCCAAGCTTTGTATTTGGATCATTGTTTCAAATTTATTGACATTTCTATGCTCGGAAAACTTGAAAAGCTTGAGATTCTTAGTATGAGAAACTCACGTTTGAAGGAGTTGTCGAGAGAAATAGCGGGTTTGACCAATCTTAAGATATTGGATATCACCGGTAAAAGTATTGATAAAATTCCATCCAAAGTGATATCCAAGTTGCATAAATTAGAAGAACTGTACATGCAGGGATTTTGGAACTGGGGGAGTAACGATGTAGGCGAAGGAGAAGAAACTAATGCTGGCTTTGACGAGGTGACTAGCTTGTCAAATTTAAGAGTTTTGGAGGTTTGTATCTCCAATGCAGAATGTATCCCTAAAGATGTTAAGTTCAATCCGAATTGGGTTAGGTTTAAAATATGTATCAGCATATCCATCAAGATGAAAGCCACCGATCAACATGGTCATAATTCAATATCCTTGGAGGTTGACACCATCAGTAGCTTACCGGACTGGTTTTGCAACGCGGTGATGACCATAGCAGAGAGGCTACAGTGGGTAGGGTGCAAAAGGCTTACTGACAGTTTTGTGGAATATGAGCATAGGAGGTTACATGAACTGAAGCATCTCTTTGTATATGGTGCCAATAGCAACGAGCTGATGAACACAAAAACATGGGTTCCAAAAGAACCTGTGTTCATGAACTTGGAAGAGTTGTATCTGATTGTCGTAGATTGCCAGGAATTATGTGTTGGTGAATTACCACCTAGGTCTTTATTCAATTTGAAGTTATTGAATGTAGAATCTTGTTATAACTGGGGGAATGTACTTTTACCATCAAAATCGTTAGAGAGACTGCCAAATATGGAAGAACTAACTTGTAGTTCCATGGAGGGGATTGAATATGTGTTTGGGCATGAAGCAAAACAATCAAATTTGAGAGAGATAAAGTTGTCTGATCTAGCTGCAGTAAGAGGTATATGTGAGGGACCTGCTCCATCTGCAATGTTCCAAACACTTGTGCGGTTGTTCATTGACCGTTGCCACTTGCATGGAAGTCTCTTCACTTCTGATGTTGCTCAATGTCTTTTTCAATTGAAGTACTTTGATGTACGACATTGCCCCTTTTTGGAAAGAATAGTGGAAGTAAGCAATAGGAAGATCATTCTTCCAAAATTGAAGGAATTAACTTTGTTACGACTTCAAATGTTGTCCTATGAAAGTGCTACTATTGATATTGAGTGTCCTTCATTGGAAAGCTTGTGTGTGTTGGACTGCCCGAAGTTTTCAGCCTCTTATTCCGATTTCCACAGCAGGAAACAAGTCGTACTCAATAGGTATCCTGTGCATCTCCTCCTTCATCAGATCGATGTGTTTTTGAGTTTCAGGGGTGCAGACACTCGCAAGGGGATCACAGTCGACATACACGATCGGCTAAATAGGAGTGGAATCAAAACATTCATGGATGAGCAGGACCTTCAAGTAGGAGATGCTATTTCTCCCACTCTCTTAGCAGCAATCAAAGATTCAAGGTTTGCAATTGTTGTTCTCTCGCAAAATTATGCTTCTTCTGCTTGGTGTCTGGAGGAACTTAGAGAGATTTGTCTGTCCATGGAGGACAACAGAATTTTGCCACTTTTTTATCATGTTGATCCTACTGATGTTCGGTATCAGAAGAGGAGTTTCGAAAAAGATTTCTCTAAGCATGAAACGTCTGGGCGACATGAATCAGAGAAAGTGAAGCAATGGAAAGCTGCTTTAAACAAAGTGGCCGGTTTTTCTGGGTGGAATACAAATGATTATAAGACTCACAAAGAGCTTGTTGACGTCATTGTGGGATTTCTCCTCAGTAAAGTAGTACCTGACGCAATTGAGTCCGCAGGAGATTTTGAAGCATTTGAAGCAACAAGACAAGCCATGAATGAGGTTATGAAGGCGCTAACAGATGACGAGGTCACTGCCGTCGGTGTCTACGGCATGGGAGGCGTTGGAAAGACAACCATGGTGAAACATGTCGGTGCACATGCCCGAAAAAGTGGGATTTTTGATCGTGTGATTATGGGTGTCGTATCCCAAAGCCCTGACTACAGAAAAATTCAAGGCGCATTAGCAGACCTGTTGGGCGTCAAATTGAAGGGAGAGACAGAAATTGGAAGAGCAGCTGGATTGAGTAAGGAGATGAAGAGAAGAAATAAGATCCTTATAATCCTGGACGACATTTGGGGGAGAACAGAGTTGTCAAAGATAGGGATTCCCAGCTACGAGGAGCTTCAGAAGTGCAAATCCAAAGTCATACTTACCACAAGGATATGGAATGTGTGTCATGTCATGAGATGCCAAGAAAAGATCACCCTCAGTACCCTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTCGAGGATGTAGCGAGGTGGGTAGCTGGAGAGTGTTGTGGTTTACCGATTGCATTGATAGCCGTCGCAAGGGCACTTGGAGATAAAGAACTGGTGGAATGGAAAAGAGCAGCTCAACGACTAGAGAAGTCGCAATCTGCCAACCCTGACGATTATGGGGAAGCATCCAAATGTATTAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAATCATACTTCTTGCTCTGTTGCCTATTCCCAGAAGACTGTGACATCCCAAAAGAAGGCTTGTTCAGGTATGCGATCGGGAAAGGATTGTTTCGAGATGCTGACACACTCGAAGAAGCCAGAGAGAGAGCTGATTCCGTGGCCACGCACCTTAAACATTCTTGCTTGCTTTTGGATAGTGAAGAAGAATATGGATGTGTAAAGATGCATGATGTTGTCCGAGATACAGCCATTCAAATTGCGCAATCTGAACATGGGTTTTTGGTGAGAGCTGGCTGTGGTTTAAAGGACTGGCCACGTCAATTACATGAAGGCTATTCTTCACTCTCACTAATGCGCAATCATATTACCAAGCTACCCGAAAAGTTTGTATGTCCAGAACTCCAGATTTTGTTACTAAACAAGGATAGGTTAATAGGTACGTGGATGCCTATAGATAAGATCTCAGATACCTTTTTTCAAACTCCCAATGAATTAAGGGTCTTAGATCTTAGCTGGACTGCTATTTCATTACTACCCCAATCGTTGTCTCCCCTAACCAACCTCCGAGCTTTGTATTTAGATGGTTGTTTCATTCTTGACACTTCCATGGTCGGAAAACTTAAGAAGCTTGAGATTCTTAGTATGAGACACAATTTTTTGATGGAGTTGTCCAGAGAAATTGGAGATTTGACCAATCTAAAGATGTTGGATTTCACTGGTGCAAGTATTCAGACAATTACATCCAAAGTGATCATGTCAAAGTTGCATAATTTAGAAGAACTGTACATGGACCGATTCTTGAACTGGGCGAAAAAAGTTGAGGGGGACAGAGAAGAAACAAATGTTGGTTTTGACGAGGTAACTAGCTTGTCAAATTTAAGAGTTTTAGAGGTTTGTTTGTTAGATGCAGAATGTATACCCAAAAATGTTGAGTTCAATCCGAATTGCGCTAAATTTGATATATATGTGGGCAGTATCGGCAGGAAGATGGAAGTCAATAATCGATATGGTCATAAATCAATGTCCTTGAGGCTTGGCACCTTAAGTAGCTTACCGGACTGGTTTTGCAATGCGGTGATGAACATAACAGAGAGGCTACGGTGGGAAGGTTGCCGAAGGTTAATTGACATATTTGTGGAGTCTGAGCATGGGAGATTACATGGACTGAAGCTTCTCTATATAATTGGTTCCAAGGAGTACAAGCTTATGAACCCAATAACATGGGTTCCCAAAAAACCTGTATTCGAGAACTTGGAAGAGTTGCATCTGGAATACGTGGATTGCCAGGAGTTATGTGTTGGTGAATTACCACCTGGGTCTCTATTCAATTTGAAGTTATTGAAGGTTCACTCTTGTAACAACTGGGGGAATGTACTTTTACCATCAACACTGTTGCAGAGACTACCAAATCTGGAAGAACTACATTGTCTGTACTTGGAGATTGAATATGTGTTTGGGTATGAAGCGTTATTTGAGCCAGAACAATCACATTTGAGAAAGATAGACTTGTATTTTCTAGATACAATAACAAGCATATGTGATGGACCTGCTCCACCTGCAATGTTCCAAAATCTTCAGAGTTTGTCCATTAGTTGTTGCGGCTTGCTGGGAAGTCTCTTCACCTCTGATGTTGCTCAATATCTTTTTCAATTGGAATACATTGGTTTACTAGGTTGCCCCTTTTTGCAAAGAATAGTTGAAGCAAGCAACAAGAAGATCATTCTTCCAAAATTGAAGGAATTAGTTTTGAAAGAACTACCAATGTTATACTATGAAAGTGCTTCTTTTGATATCGAGTGTCCTTCACTGGAAAACCTATATGTGATGGACTGCCCCATGTTTTTAGCCTCTTCTCTTGACTTCCACAGCAGGAAACAAGTCCAATTCAAGAGGTATGTTGCTGTATCAAAAACTTGA

Protein Analysis

2131

Amino Acids

243.05

Weight (kDa)

6.21

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 186 1.1e-37 TIR domain
TIR_2 PF13676 36 - 121 3.7e-13 TIR domain
NB-ARC PF00931 203 - 367 6.9e-31 NB-ARC domain
LRR_8 PF13855 576 - 634 1.1e-08 Leucine rich repeat
LRR_14 PF23598 639 - 736 3.2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 855 - 993 4e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 932 - 1048 2.1e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
TIR PF01582 1076 - 1234 2.2e-41 TIR domain
TIR_2 PF13676 1077 - 1172 4.2e-16 TIR domain
NB-ARC PF00931 1257 - 1420 1.2e-29 NB-ARC domain
LRR_14 PF23598 1658 - 1791 1.5e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 1911 - 2049 2.3e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 498, 899, 2670, 3660, 4061
AarI CACCTGC 1 cut(s) 6043
AasI GACNNNNNNGTC 1 cut(s) 2182
AatII GACGTC 1 cut(s) 3687
Acc16I TGCGCA 3 cut(s) 1593, 4740, 4835
Acc36I ACCTGC 3 cut(s) 2857, 6034, 6043
AccB1I GGYRCC 2 cut(s) 2498, 5516
AccI GTMKAC 7 cut(s) 108, 415, 666, 1165, 3276, 3828, 5395
AccII CGCG 1 cut(s) 2384
AciI CCGC 8 cut(s) 541, 1687, 2000, 2384, 2881, 3735, 5552, 6078
AclWI GGATC 7 cut(s) 107, 886, 1768, 1902, 3275, 3503, 4048
AcoI YGGCCR 5 cut(s) 1476, 1643, 3627, 4638, 4790
AcuI CTGAAG 5 cut(s) 1620, 2498, 4112, 5666, 6038
AcvI CACGTG 1 cut(s) 340
AcyI GRCGYC 3 cut(s) 522, 3684, 3981
AfiI CCNNNNNNNGG 4 cut(s) 2751, 2986, 4353, 4553
AflII CTTAAG 3 cut(s) 2015, 5099, 5521
AflIII ACRYGT 2 cut(s) 706, 3868
AgeI ACCGGT 1 cut(s) 2033
AhdI GACNNNNNGTC 1 cut(s) 1336
AhlI ACTAGT 1 cut(s) 1832
AjiI CACGTC 2 cut(s) 1649, 4796
AjnI CCWGG 5 cut(s) 903, 2595, 4065, 5763, 5791
AjuI GAANNNNNNNTTGG 8 cut(s) 2592, 2624, 2751, 2783, 5307, 5339, 5916, 5948
AloI GAACNNNNNNTCC 2 cut(s) 1382, 1414
Alw21I GWGCWC 2 cut(s) 718, 3880
Alw44I GTGCAC 2 cut(s) 714, 3876
AlwI GGATC 7 cut(s) 107, 886, 1768, 1902, 3275, 3503, 4048
AoxI GGCC 5 cut(s) 1476, 1643, 3627, 4638, 4790
ApaLI GTGCAC 2 cut(s) 714, 3876
ApeKI GCWGC 7 cut(s) 203, 854, 2822, 3371, 3611, 4016, 4394
ArsI GACNNNNNNTTYG 4 cut(s) 1822, 1854, 4131, 4163
AsiGI ACCGGT 1 cut(s) 2033
AspA2I CCTAGG 1 cut(s) 2624
AspLEI GCGC 7 cut(s) 634, 1594, 3796, 3962, 4741, 4836, 5429
AspS9I GGNCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
AsuC2I CCSGG 1 cut(s) 1571
AsuII TTCGAA 3 cut(s) 381, 822, 3543
AvaII GGWCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
AvrII CCTAGG 1 cut(s) 2624
BaeGI GKGCMC 4 cut(s) 718, 1198, 3880, 4360
BalI TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
BanI GGYRCC 2 cut(s) 2498, 5516
BbrPI CACGTG 1 cut(s) 340
BbsI GAAGAC 3 cut(s) 842, 1377, 4539
Bbv12I GWGCWC 2 cut(s) 718, 3880
BbvI GCAGC 7 cut(s) 215, 866, 2809, 3383, 3598, 4028, 4406
BceAI ACGGC 5 cut(s) 331, 685, 3803, 3847, 4331
BcgI CGANNNNNNTGC 2 cut(s) 3797, 3831
BciT130I CCWGG 5 cut(s) 905, 2597, 4067, 5765, 5793
BciVI GTATCC 5 cut(s) 1064, 1567, 2240, 3207, 3937
BclI TGATCA 1 cut(s) 5229
BcnI CCSGG 1 cut(s) 1571
BcuI ACTAGT 1 cut(s) 1832
BfmI CTRYAG 5 cut(s) 1671, 2408, 2823, 3943, 4929
BfoI RGCGCY 2 cut(s) 635, 3797
BfrI CTTAAG 3 cut(s) 2015, 5099, 5521
BfuAI ACCTGC 3 cut(s) 2857, 6034, 6043
BfuI GTATCC 5 cut(s) 1064, 1567, 2240, 3207, 3937
BglII AGATCT 4 cut(s) 288, 1819, 4938, 4984
BisI GCNGC 8 cut(s) 204, 855, 2823, 3372, 3612, 4017, 4395, 6079
BlnI CCTAGG 1 cut(s) 2624
BlsI GCNGC 8 cut(s) 205, 856, 2824, 3373, 3613, 4018, 4396, 6080
BmcAI AGTACT 2 cut(s) 987, 2960
Bme1390I CCNGG 6 cut(s) 905, 1571, 2597, 4067, 5765, 5793
Bme18I GGWCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
BmeRI GACNNNNNGTC 1 cut(s) 1336
BmgBI CACGTC 2 cut(s) 1649, 4796
BmgT120I GGNCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
BmiI GGNNCC 8 cut(s) 345, 571, 2500, 2538, 2847, 5518, 5668, 5706
BmrFI CCNGG 6 cut(s) 905, 1571, 2597, 4067, 5765, 5793
BmrI ACTGGG 5 cut(s) 67, 2128, 2683, 5293, 5851
BmuI ACTGGG 5 cut(s) 67, 2128, 2683, 5293, 5851
BpiI GAAGAC 3 cut(s) 842, 1377, 4539
BpmI CTGGAG 3 cut(s) 3461, 4332, 4865
Bpu14I TTCGAA 3 cut(s) 381, 822, 3543
BpuEI CTTGAG 3 cut(s) 1967, 5129, 5527
BpuMI CCSGG 1 cut(s) 1571
Bsa29I ATCGAT 2 cut(s) 3225, 5482
BsaAI YACGTR 3 cut(s) 340, 4920, 5755
BsaHI GRCGYC 3 cut(s) 522, 3684, 3981
BsaI GGTCTC 1 cut(s) 5802
BsaWI WCCGGW 3 cut(s) 2033, 2365, 5533
BsaXI ACNNNNNCTCC 2 cut(s) 1382, 1412
Bsc4I CCNNNNNNNGG 4 cut(s) 2751, 2986, 4353, 4553
Bse118I RCCGGY 2 cut(s) 2033, 3629
Bse3DI GCAATG 5 cut(s) 2868, 2974, 3608, 5554, 6045
BseBI CCWGG 5 cut(s) 905, 2597, 4067, 5765, 5793
BseCI ATCGAT 2 cut(s) 3225, 5482
BseLI CCNNNNNNNGG 4 cut(s) 2751, 2986, 4353, 4553
BseMI GCAATG 5 cut(s) 2868, 2974, 3608, 5554, 6045
BseMII CTCAG 5 cut(s) 1063, 3718, 4225, 4956, 5613
BseRI GAGGAG 7 cut(s) 388, 693, 1153, 3200, 3550, 3692, 4136
BseSI GKGCMC 4 cut(s) 718, 1198, 3880, 4360
BseXI GCAGC 7 cut(s) 215, 866, 2809, 3383, 3598, 4028, 4406
BseYI CCCAGC 3 cut(s) 5, 4112, 6085
BsgI GTGCAG 1 cut(s) 3270
Bsh1236I CGCG 1 cut(s) 2384
Bsh1285I CGRYCG 4 cut(s) 121, 1413, 3289, 4575
BshFI GGCC 5 cut(s) 1478, 1645, 3629, 4640, 4792
BshNI GGYRCC 2 cut(s) 2498, 5516
BshTI ACCGGT 1 cut(s) 2033
BshVI ATCGAT 2 cut(s) 3225, 5482
BsiEI CGRYCG 4 cut(s) 121, 1413, 3289, 4575
BsiHKAI GWGCWC 2 cut(s) 718, 3880
BsiSI CCGG 6 cut(s) 1570, 1828, 2034, 2366, 3630, 5534
BslFI GGGAC 3 cut(s) 1782, 2859, 5318
BslI CCNNNNNNNGG 4 cut(s) 2751, 2986, 4353, 4553
BsmFI GGGAC 3 cut(s) 1782, 2859, 5318
BsmI GAATGC 1 cut(s) 1160
BsnI GGCC 5 cut(s) 1478, 1645, 3629, 4640, 4792
Bso31I GGTCTC 1 cut(s) 5802
Bsp119I TTCGAA 3 cut(s) 381, 822, 3543
Bsp1286I GDGCHC 4 cut(s) 718, 1198, 3880, 4360
Bsp1407I TGTACA 2 cut(s) 2097, 5262
Bsp19I CCATGG 6 cut(s) 612, 696, 2745, 3468, 3858, 5085
BspACI CCGC 8 cut(s) 541, 1687, 2000, 2384, 2881, 3735, 5552, 6078
BspANI GGCC 5 cut(s) 1478, 1645, 3629, 4640, 4792
BspCNI CTCAG 5 cut(s) 1062, 3717, 4224, 4955, 5614
BspDI ATCGAT 2 cut(s) 3225, 5482
BspFNI CGCG 1 cut(s) 2384
BspHI TCATGA 3 cut(s) 1023, 2556, 4185
BspLI GGNNCC 8 cut(s) 345, 571, 2500, 2538, 2847, 5518, 5668, 5706
BspMAI CTGCAG 2 cut(s) 1675, 2827
BspMI ACCTGC 3 cut(s) 2857, 6034, 6043
BspPI GGATC 7 cut(s) 107, 886, 1768, 1902, 3275, 3503, 4048
BspQI GCTCTTC 2 cut(s) 844, 4006
BspT104I TTCGAA 3 cut(s) 381, 822, 3543
BspT107I GGYRCC 2 cut(s) 2498, 5516
BspTI CTTAAG 3 cut(s) 2015, 5099, 5521
BspTNI GGTCTC 1 cut(s) 5802
BsrDI GCAATG 5 cut(s) 2868, 2974, 3608, 5554, 6045
BsrFI RCCGGY 2 cut(s) 2033, 3629
BsrGI TGTACA 2 cut(s) 2097, 5262
BssAI RCCGGY 2 cut(s) 2033, 3629
BssNAI GTATAC 1 cut(s) 5396
BssNI GRCGYC 3 cut(s) 522, 3684, 3981
BssT1I CCWWGG 9 cut(s) 612, 696, 2337, 2624, 2745, 3468, 3858, 5085, 5670
Bst1107I GTATAC 1 cut(s) 5396
Bst2UI CCWGG 5 cut(s) 905, 2597, 4067, 5765, 5793
BstACI GRCGYC 3 cut(s) 522, 3684, 3981
BstAFI CTTAAG 3 cut(s) 2015, 5099, 5521
BstAUI TGTACA 2 cut(s) 2097, 5262
BstBAI YACGTR 3 cut(s) 340, 4920, 5755
BstBI TTCGAA 3 cut(s) 381, 822, 3543
BstC8I GCNNGC 9 cut(s) 225, 1502, 1624, 1703, 2161, 4664, 4771, 6083, 6184
BstDSI CCRYGG 8 cut(s) 612, 696, 1473, 2745, 3468, 3858, 4635, 5085
BstFNI CGCG 1 cut(s) 2384
BstH2I RGCGCY 2 cut(s) 635, 3797
BstHHI GCGC 7 cut(s) 634, 1594, 3796, 3962, 4741, 4836, 5429
BstMCI CGRYCG 4 cut(s) 121, 1413, 3289, 4575
BstMWI GCNNNNNNNGC 7 cut(s) 2858, 3428, 3758, 3840, 4343, 5457, 6035
BstNI CCWGG 5 cut(s) 905, 2597, 4067, 5765, 5793
BstNSI RCATGY 4 cut(s) 710, 2104, 3872, 3884
BstSCI CCNGG 6 cut(s) 903, 1569, 2595, 4065, 5763, 5791
BstSFI CTRYAG 5 cut(s) 1671, 2408, 2823, 3943, 4929
BstSLI GKGCMC 4 cut(s) 718, 1198, 3880, 4360
BstUI CGCG 1 cut(s) 2384
BstV1I GCAGC 7 cut(s) 215, 866, 2809, 3383, 3598, 4028, 4406
BstV2I GAAGAC 3 cut(s) 842, 1377, 4539
BstX2I RGATCY 7 cut(s) 288, 891, 1773, 1819, 4053, 4938, 4984
BstXI CCANNNNNNTGG 2 cut(s) 2909, 5700
BstYI RGATCY 7 cut(s) 288, 891, 1773, 1819, 4053, 4938, 4984
BstZ17I GTATAC 1 cut(s) 5396
Bsu15I ATCGAT 2 cut(s) 3225, 5482
BsuI GTATCC 5 cut(s) 1064, 1567, 2240, 3207, 3937
BsuRI GGCC 5 cut(s) 1478, 1645, 3629, 4640, 4792
BsuTUI ATCGAT 2 cut(s) 3225, 5482
BtgI CCRYGG 8 cut(s) 612, 696, 1473, 2745, 3468, 3858, 4635, 5085
BtrI CACGTC 2 cut(s) 1649, 4796
BtsI GCAGTG 3 cut(s) 446, 1668, 3813
BtsIMutI CAGTG 7 cut(s) 446, 1668, 2417, 3813, 5193, 5867, 6284
BveI ACCTGC 3 cut(s) 2857, 6034, 6043
Cac8I GCNNGC 9 cut(s) 225, 1502, 1624, 1703, 2161, 4664, 4771, 6083, 6184
CciI TCATGA 3 cut(s) 1023, 2556, 4185
CfoI GCGC 7 cut(s) 634, 1594, 3796, 3962, 4741, 4836, 5429
Cfr10I RCCGGY 2 cut(s) 2033, 3629
Cfr13I GGNCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
ClaI ATCGAT 2 cut(s) 3225, 5482
CseI GACGC 3 cut(s) 530, 3731, 3970
CspAI ACCGGT 1 cut(s) 2033
DraI TTTAAA 4 cut(s) 1635, 2272, 3618, 4782
DrdI GACNNNNNNGTC 1 cut(s) 2182
DriI GACNNNNNGTC 1 cut(s) 1336
DseDI GACNNNNNNGTC 1 cut(s) 2182
EaeI YGGCCR 5 cut(s) 1476, 1643, 3627, 4638, 4790
Eam1105I GACNNNNNGTC 1 cut(s) 1336
Eco130I CCWWGG 9 cut(s) 612, 696, 2337, 2624, 2745, 3468, 3858, 5085, 5670
Eco31I GGTCTC 1 cut(s) 5802
Eco32I GATATC 4 cut(s) 362, 2029, 2070, 6271
Eco47I GGWCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
Eco57I CTGAAG 5 cut(s) 1620, 2498, 4112, 5666, 6038
Eco72I CACGTG 1 cut(s) 340
EcoO109I RGGNCCY 3 cut(s) 163, 2846, 3331
EcoRII CCWGG 5 cut(s) 903, 2595, 4065, 5763, 5791
EcoRV GATATC 4 cut(s) 362, 2029, 2070, 6271
EcoT14I CCWWGG 9 cut(s) 612, 696, 2337, 2624, 2745, 3468, 3858, 5085, 5670
EcoT22I ATGCAT 2 cut(s) 1560, 4707
ErhI CCWWGG 9 cut(s) 612, 696, 2337, 2624, 2745, 3468, 3858, 5085, 5670
FaqI GGGAC 3 cut(s) 1782, 2859, 5318
FauI CCCGC 1 cut(s) 1993
FauNDI CATATG 1 cut(s) 6014
FbaI TGATCA 1 cut(s) 5229
FblI GTMKAC 7 cut(s) 108, 415, 666, 1165, 3276, 3828, 5395
Fnu4HI GCNGC 8 cut(s) 204, 855, 2823, 3372, 3612, 4017, 4395, 6079
Fsp4HI GCNGC 8 cut(s) 204, 855, 2823, 3372, 3612, 4017, 4395, 6079
FspI TGCGCA 3 cut(s) 1593, 4740, 4835
GlaI GCGC 7 cut(s) 633, 1593, 3795, 3961, 4740, 4835, 5428
GluI GCNGC 8 cut(s) 204, 855, 2823, 3372, 3612, 4017, 4395, 6079
GsaI CCCAGC 3 cut(s) 9, 4116, 6089
GsuI CTGGAG 3 cut(s) 3461, 4332, 4865
HaeII RGCGCY 2 cut(s) 635, 3797
HaeIII GGCC 5 cut(s) 1478, 1645, 3629, 4640, 4792
HapII CCGG 6 cut(s) 1570, 1828, 2034, 2366, 3630, 5534
HgaI GACGC 3 cut(s) 530, 3731, 3970
HhaI GCGC 7 cut(s) 634, 1594, 3796, 3962, 4741, 4836, 5429
Hin1I GRCGYC 3 cut(s) 522, 3684, 3981
Hin6I GCGC 7 cut(s) 632, 1592, 3794, 3960, 4739, 4834, 5427
HinP1I GCGC 7 cut(s) 632, 1592, 3794, 3960, 4739, 4834, 5427
HincII GTYRAC 6 cut(s) 109, 416, 520, 2347, 3277, 3682
HindII GTYRAC 6 cut(s) 109, 416, 520, 2347, 3277, 3682
HindIII AAGCTT 7 cut(s) 387, 1881, 1943, 3115, 5105, 5648, 5681
HpaII CCGG 6 cut(s) 1570, 1828, 2034, 2366, 3630, 5534
Hpy99I CGWCG 2 cut(s) 3824, 4352
HpyCH4IV ACGT 8 cut(s) 339, 1648, 1972, 3569, 3684, 4795, 4919, 5754
HpyF10VI GCNNNNNNNGC 7 cut(s) 2858, 3428, 3758, 3840, 4343, 5457, 6035
HpySE526I ACGT 8 cut(s) 339, 1648, 1972, 3569, 3684, 4795, 4919, 5754
Hsp92I GRCGYC 3 cut(s) 522, 3684, 3981
HspAI GCGC 7 cut(s) 632, 1592, 3794, 3960, 4739, 4834, 5427
Ksp22I TGATCA 1 cut(s) 5229
LguI GCTCTTC 2 cut(s) 844, 4006
LmnI GCTCC 4 cut(s) 343, 2857, 4123, 6034
Lsp1109I GCAGC 7 cut(s) 215, 866, 2809, 3383, 3598, 4028, 4406
MaeII ACGT 8 cut(s) 339, 1648, 1972, 3569, 3684, 4795, 4919, 5754
MfeI CAATTG 6 cut(s) 228, 564, 2951, 3396, 3726, 6128
MflI RGATCY 7 cut(s) 288, 891, 1773, 1819, 4053, 4938, 4984
MhlI GDGCHC 4 cut(s) 718, 1198, 3880, 4360
MlsI TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
MluNI TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
MlyI GAGTC 5 cut(s) 496, 1367, 3658, 3740, 5627
MmeI TCCRAC 5 cut(s) 664, 3108, 3826, 5071, 5166
Mox20I TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
Mph1103I ATGCAT 2 cut(s) 1560, 4707
MscI TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
MslI CAYNNNNRTG 9 cut(s) 711, 1291, 1922, 2297, 2907, 3873, 4453, 5084, 5612
Msp20I TGGCCA 4 cut(s) 1478, 1645, 4640, 4792
MspA1I CMGCKG 1 cut(s) 4019
MspCI CTTAAG 3 cut(s) 2015, 5099, 5521
MspI CCGG 6 cut(s) 1570, 1828, 2034, 2366, 3630, 5534
MspR9I CCNGG 6 cut(s) 905, 1571, 2597, 4067, 5765, 5793
MunI CAATTG 6 cut(s) 228, 564, 2951, 3396, 3726, 6128
Mva1269I GAATGC 1 cut(s) 1160
MvaI CCWGG 5 cut(s) 905, 2597, 4067, 5765, 5793
MvnI CGCG 1 cut(s) 2384
MwoI GCNNNNNNNGC 7 cut(s) 2858, 3428, 3758, 3840, 4343, 5457, 6035
NciI CCSGG 1 cut(s) 1571
NcoI CCATGG 6 cut(s) 612, 696, 2745, 3468, 3858, 5085
NdeI CATATG 1 cut(s) 6014
NlaIV GGNNCC 8 cut(s) 345, 571, 2500, 2538, 2847, 5518, 5668, 5706
NmuCI GTSAC 5 cut(s) 53, 649, 2173, 3811, 4538
NsbI TGCGCA 3 cut(s) 1593, 4740, 4835
NsiI ATGCAT 2 cut(s) 1560, 4707
NspI RCATGY 4 cut(s) 710, 2104, 3872, 3884
NspV TTCGAA 3 cut(s) 381, 822, 3543
PagI TCATGA 3 cut(s) 1023, 2556, 4185
PaqCI CACCTGC 1 cut(s) 6043
PciI ACATGT 2 cut(s) 706, 3868
PciSI GCTCTTC 2 cut(s) 844, 4006
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 2136, 3282
PctI GAATGC 1 cut(s) 1160
PflFI GACNNNGTC 2 cut(s) 647, 3809
PfoI TCCNGGA 3 cut(s) 903, 1569, 4065
PinAI ACCGGT 1 cut(s) 2033
PkrI GCNGC 8 cut(s) 205, 856, 2824, 3373, 3613, 4018, 4396, 6080
Ple19I CGATCG 4 cut(s) 121, 1413, 3289, 4575
PleI GAGTC 5 cut(s) 496, 1367, 3658, 3739, 5626
PmaCI CACGTG 1 cut(s) 340
PmlI CACGTG 1 cut(s) 340
PpsI GAGTC 5 cut(s) 496, 1367, 3658, 3739, 5626
Ppu21I YACGTR 3 cut(s) 340, 4920, 5755
PpuMI RGGWCCY 3 cut(s) 163, 2846, 3331
PscI ACATGT 2 cut(s) 706, 3868
PsiI TTATAA 5 cut(s) 498, 899, 2670, 3660, 4061
Psp5II RGGWCCY 3 cut(s) 163, 2846, 3331
Psp6I CCWGG 5 cut(s) 903, 2595, 4065, 5763, 5791
PspCI CACGTG 1 cut(s) 340
PspFI CCCAGC 3 cut(s) 5, 4112, 6085
PspGI CCWGG 5 cut(s) 903, 2595, 4065, 5763, 5791
PspN4I GGNNCC 8 cut(s) 345, 571, 2500, 2538, 2847, 5518, 5668, 5706
PspPI GGNCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
PspPPI RGGWCCY 3 cut(s) 163, 2846, 3331
PstI CTGCAG 2 cut(s) 1675, 2827
PsuI RGATCY 7 cut(s) 288, 891, 1773, 1819, 4053, 4938, 4984
PsyI GACNNNGTC 2 cut(s) 647, 3809
PvuI CGATCG 4 cut(s) 121, 1413, 3289, 4575
PvuII CAGCTG 1 cut(s) 4019
RseI CAYNNNNRTG 9 cut(s) 711, 1291, 1922, 2297, 2907, 3873, 4453, 5084, 5612
SalI GTCGAC 3 cut(s) 107, 414, 3275
SapI GCTCTTC 2 cut(s) 844, 4006
SatI GCNGC 8 cut(s) 204, 855, 2823, 3372, 3612, 4017, 4395, 6079
Sau96I GGNCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
ScaI AGTACT 2 cut(s) 987, 2960
SchI GAGTC 5 cut(s) 496, 1367, 3658, 3740, 5627
ScrFI CCNGG 6 cut(s) 905, 1571, 2597, 4067, 5765, 5793
SduI GDGCHC 4 cut(s) 718, 1198, 3880, 4360
SfcI CTRYAG 5 cut(s) 1671, 2408, 2823, 3943, 4929
SfuI TTCGAA 3 cut(s) 381, 822, 3543
SinI GGWCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
SmiMI CAYNNNNRTG 9 cut(s) 711, 1291, 1922, 2297, 2907, 3873, 4453, 5084, 5612
SmlI CTYRAG 6 cut(s) 1946, 2015, 5099, 5108, 5506, 5521
SmoI CTYRAG 6 cut(s) 1946, 2015, 5099, 5108, 5506, 5521
SpeI ACTAGT 1 cut(s) 1832
SsiI CCGC 8 cut(s) 541, 1687, 2000, 2384, 2881, 3735, 5552, 6078
StyD4I CCNGG 6 cut(s) 903, 1569, 2595, 4065, 5763, 5791
StyI CCWWGG 9 cut(s) 612, 696, 2337, 2624, 2745, 3468, 3858, 5085, 5670
TaiI ACGT 8 cut(s) 342, 1651, 1975, 3572, 3687, 4798, 4922, 5757
TaqII GACCGA 1 cut(s) 5286
TatI WGTACW 8 cut(s) 985, 2097, 2683, 2958, 5262, 5676, 5851, 5910
TauI GCSGC 1 cut(s) 6081
TscAI CASTG 7 cut(s) 446, 1675, 2417, 3820, 5200, 5874, 6291
TseFI GTSAC 5 cut(s) 53, 649, 2173, 3811, 4538
TseI GCWGC 7 cut(s) 203, 854, 2822, 3371, 3611, 4016, 4394
Tsp45I GTSAC 5 cut(s) 53, 649, 2173, 3811, 4538
TspGWI ACGGA 3 cut(s) 1293, 1462, 4624
TspRI CASTG 7 cut(s) 446, 1675, 2417, 3820, 5200, 5874, 6291
Tth111I GACNNNGTC 2 cut(s) 647, 3809
Vha464I CTTAAG 3 cut(s) 2015, 5099, 5521
VneI GTGCAC 2 cut(s) 714, 3876
VpaK11BI GGWCC 8 cut(s) 163, 570, 1077, 2846, 3331, 4239, 5269, 6023
XbaI TCTAGA 1 cut(s) 5995
XceI RCATGY 4 cut(s) 710, 2104, 3872, 3884
XmaJI CCTAGG 1 cut(s) 2624
XmiI GTMKAC 7 cut(s) 108, 415, 666, 1165, 3276, 3828, 5395
ZraI GACGTC 1 cut(s) 3685
ZrmI AGTACT 2 cut(s) 987, 2960
Zsp2I ATGCAT 2 cut(s) 1560, 4707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.