FvH4_3g43631

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018015
Physical Location & Seq
Reverse (-)
36347922 .. 36352141
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43631.t1

Sequence Viewer

Length: 3705 bp
ATGGCCTCCAGCTCCCAAATACCCTGTGCATATCCTCCTCCTTCAGATCCATGTTGGAGGTATGATGTGTTTTTGAGTTTCAGGGGCGTTGACACTCGCAAGGGTATCACAATCGACATATACGATGGACTCAATCGGAGAGGCATTAAAACATTCATGGATGATCCAGATCTTAAAGTCGGGGACACTATTTCTCGCACGCTCATAGAAGCAATTGAAGGGTCAAGGTTTGCAATTGTTGTTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATCCATGGAAGACAACAGAATTTTTCCACTTTTTTATCATGTTGAGCCTACTGATGTTCGATATCAGAAGCAGAGTTTCGAAGAAGCTTTCTCTAAACATGAAAGCTCTGGGAGATATGAATCAGAGAAGGTGAATCATTGGAAAGCTGCTTTAAACAAAGTGGCCAATATATCCGGGTGGAATACGCAGAATTATAAGACTCACAAAGAACTTGTTAACGCCATTGTGGATGTTCTCATCAGTAGGGCAGTACCTGAGGCAATTGAGTCCACAGCAGATTTCCAAGCATTTGAAGCAACAAGAGTAGCCATGGATGAGGTTATGAAGGCCCTTAAAGATGACGAGGTCACTGCCGTTGGTGTCTACGGCATGGGAGGTGTGGGCAAGACAACCTTGGTGGAACATGTCGGTGCACAAGCTCGGAAAAGTGGGAGTTTTGATTATGTGACTAAGTGTGTCGTATCCCAAAATCCTGGTTTTGAAATCCTTCAAGACGCATTGGCAGAGCAGTTGGGCTTCAAATTACATGAGGAAACAGAAATTGGAAGATCCACTAGGTTGCATAAGGAGATAATGAGACGAACAAAGATTCTTATAATCCTGGACGATATTTGGGAGGGAATGAAGTTATCAAGAGTAGGAATTCCAAGCCACAAGGAACTTCAAAAGTGTAATTCCAAAATCCTACTCACCACAAGGATAAGGAATGTTTGTCATGCCATGGAGTGCCAAAGAAAGATCACACTCAACATTCTCTCAGAACAAGATTCTTGGGCCTTGTTTGTGAGTAAAGTAAGAAGGTCTTTTGAGTCGTCCACTTTCGAGGGTGTGGCAAAGAAGGTTGCTGGAGAATGTAGAGGTCTACCGATTGCATTGGTCACAGTTGCAAGGGCGCTCGGAGATAAAGAGTTGGTGGAATGGGAGATAGCAGCTCTCCGGCTAAAGAAGTCGCGAACTGCCAACCCTGACGATTATGGAGAAGCAACAAAATGCATAAAATTAAGCTATGATTACTTGAAAAATGAGGACTACAAGTCATACTTCTTACTTTGTTGCTTATTCCCAGAAGACTATGGCATCCCTATAAAAGACTTGTTCAGGTATGGGATCAGGACAAGATTGTTTCGAGATGCCGAAACACTCTTGGAGGCAAGAAAGCAAGTTGATTCCGTTGTCAAGCATCTTAAATATTCTTGCTTGCTTTTGGATAGTAAAACAGATGGATTTGTAAGGATGCATGAAGTTGTCCGGGATGCAGCCATTCAAATTGTGCTATCTGAAGATGAGTTTTTTGTGAAAGCCGGCTGTCGTTTAGAGGATTGGCCACGTAATTTACATAAAGGCTACCTTGCAATCTCACTAATGAGGAATTATATCAGGGAGCTTCCCAAAAATCTGGTATGTCCAAAACTCCAGATTTTGTTACTAAACCATAATATCTTTTTAGAACATATACCAGAAACATTCTTCCAATGTCCAAATGAATTAAGGGTATTAGATCTTAGCTGGACTACTAGCCTTTCATTACTGCCCAAATCATTAGGTCTCCTAACCAACCTCACAGCTTTGTATTTAGATAATTGTGACCATCTAAGCATTGACACTTCCATACTGGGAAAACTTAAAAAGCTTGAGATGCTGAGTATGAGAAATTCACGTCTGTATGAGTTGTCGGTAGAAATAGGAGGTTTGACCAATCTGAGGATGTTGGATATTACTGGTGAACATATTTCTAGAATTCCCTCCAAACTGTTATCAAAGTTGCATAATTTAGAAGAACTGTATATGCAAGGATTTTGGGAGTGGGAGCGCTTGGAGCCTAACGCTGAGGGAGAAGAAGGAGAAGTTAATGATACCTTTACTAATGCTAGCTTTGACGAGGTAACTATGCAAGGATTTTGGGAGTGGGAGTGCTTGGAGCTTAACGCTGAGGGAGAAGAAGGAGAAGTCAATGATACCTTTACTAATGCTAGCTTTGACGAGGTAACTAGCTTGTCAAAATTAAGAGTTTTGAAGGTTACTATCAGGGGTGTTGAACATATCCCTAAATATGTTGAGCAGAAAATGAATTTGGTTGAGTTTGATATATGTATCGGTGGTTTATACGAGCCATACAAGACGAAGTTATATGATCGACATCGTCATATCTCAATACTCTTGGATGTCATGATCAGTCACTTACCGGAGTGGTTTCGTGACGAGATAATGAAGAAAGCAGAGACATTTTCCGACTCTTGGTGCCACAGGTTAATCGACATATTTGTGGAATCTGAGCATGGGAGGCTACAGAGACTGAAGCATCTGCATATAGTGGGTTACAGCAGTCGAGACATCACGGAGGTGGATCCTGCAATAACATGGGTTCCAAGAAAGCCTGTGTTCGAGAATTTGGAAGAGTTGTATATGGAGGGACTGGATTGTGCAGAGTTACGTGCCGTTCAATTTCTACCATGTGGGTCTCTATTCAAGTTGAAGGTATTGAAGGTAGAATATTGTTACAACTGGTGGAATATACTTTTACCGTCAACATTGTTACATAGACTACCAAATTTGGAAGTATTAATTGTTTGTCAGACGGACGGGAGTGAATATATGTTTGGGTATGAAGCCTTGCTTGTGTTGCAACAATTAAAGTTGAAAAAGATAGAATTGTTTGCTCTAGCAACAGTAAGCATATGTGAGGGACCTGCTCCGCCTGCGATGCTCCAGAATCTTCAAAGTTTGTCCATTACCAAATGCAAGTTGGAGGGAAGTCTCTTCACCTCTGATGTTGCTCAGTGTCTTTTTCAGTTGAATTCCTTAGAATTAGCAGTATGCCCCTTGTTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGATAATCCTTCCAAAGTTGGAGCAATTAAGTTTGACGGAACTTCCAATGTTATACAAAAGTGCTACTTTTGATATTGAGTGTCCTTCATTGGAGAACATGTCTCTGAAGGGTTGTTGGTTTCCATGGGAACAATGGAGACTGAGAAAGATAGCATTGTCCACAGTTAGCATATGTGATGGACCTTCTCGACTCCCACCTGCAATGTTCCAGAATCTTCAAAGTTTGTCCATAGAGTGGTGCAAGTCAGATGGAAGTCTCTTCACCTATGATGTTGCTCAGTGTCTTTCCCAACTGAATTCCTTAAAATTCAAGAATTGCCCTCTGTTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGACCGTCCTTCCAAAATTGAAGCGATTACATTTGGAGGTACTTCCAATGTTGTACTATGAAAGTGATACTTTTGATATTGTGTGTCCTGAATTGGAAGAGTTCTTTGTGAGTAGCTGCCCCAAGTTTTCAGCCTCTTCATCTGACTTCCACAGCAGGAAACAAGTCCAATTCATATGGAGGCCGAGTTGA

Protein Analysis

1235

Amino Acids

141.49

Weight (kDa)

5.75

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 183 5.7e-43 TIR domain
TIR_2 PF13676 23 - 117 6.7e-15 TIR domain
NB-ARC PF00931 204 - 367 3.4e-32 NB-ARC domain
LRR_14 PF23598 597 - 698 4.3e-09 Leucine-rich repeat region
LRR_8 PF13855 619 - 678 2.3e-06 Leucine rich repeat
LRR_RPS2 PF23247 920 - 1040 3.7e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 991 - 1071 4.2e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1107 - 1214 1.8e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 498, 899
AarI CACCTGC 1 cut(s) 3364
Acc36I ACCTGC 2 cut(s) 2998, 3364
AccB1I GGYRCC 1 cut(s) 2541
AccB7I CCANNNNNTGG 1 cut(s) 3393
AccI GTMKAC 2 cut(s) 666, 1165
AccII CGCG 1 cut(s) 1255
AciI CCGC 1 cut(s) 2996
AclWI GGATC 6 cut(s) 41, 158, 846, 1418, 2642, 2655
AcoI YGGCCR 2 cut(s) 465, 1625
AcuI CTGAAG 4 cut(s) 27, 1602, 2618, 3284
AfaI GTAC 3 cut(s) 555, 3555, 3569
AfeI AGCGCT 1 cut(s) 2114
AfiI CCNNNNNNNGG 5 cut(s) 2004, 2538, 3127, 3393, 3484
AflIII ACRYGT 2 cut(s) 706, 3255
AhdI GACNNNNNGTC 1 cut(s) 1336
AjiI CACGTC 1 cut(s) 1961
AjnI CCWGG 2 cut(s) 775, 903
AjuI GAANNNNNNNTTGG 6 cut(s) 828, 860, 2357, 2389, 2883, 2915
AleI CACNNNNGTG 1 cut(s) 2642
Alw21I GWGCWC 1 cut(s) 718
Alw44I GTGCAC 1 cut(s) 714
AlwI GGATC 6 cut(s) 41, 158, 846, 1418, 2642, 2655
AlwNI CAGNNNCTG 2 cut(s) 557, 2596
Aor51HI AGCGCT 1 cut(s) 2114
AoxI GGCC 6 cut(s) 3, 465, 630, 1077, 1625, 3695
ApaLI GTGCAC 1 cut(s) 714
ApeKI GCWGC 4 cut(s) 449, 1232, 1559, 3630
AseI ATTAAT 1 cut(s) 2864
Asp700I GAANNNNTTC 1 cut(s) 789
AspLEI GCGC 2 cut(s) 1198, 2115
AspS9I GGNCC 4 cut(s) 631, 1077, 2987, 3338
AsuC2I CCSGG 2 cut(s) 478, 1553
AsuHPI GGTGA 5 cut(s) 445, 985, 2036, 3055, 3412
AsuII TTCGAA 1 cut(s) 381
AsuNHI GCTAGC 2 cut(s) 2171, 2273
AvaII GGWCC 2 cut(s) 2987, 3338
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 718
BalI TGGCCA 2 cut(s) 467, 1627
BamHI GGATCC 1 cut(s) 2647
BanI GGYRCC 1 cut(s) 2541
BbsI GAAGAC 3 cut(s) 318, 1377, 3521
Bbv12I GWGCWC 1 cut(s) 718
BbvCI CCTCAGC 2 cut(s) 2130, 2232
BbvI GCAGC 4 cut(s) 436, 1244, 1571, 3617
BccI CCATC 5 cut(s) 119, 1517, 1899, 3329, 3401
BceAI ACGGC 3 cut(s) 641, 685, 2723
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 2 cut(s) 777, 905
BciVI GTATCC 1 cut(s) 775
BclI TGATCA 1 cut(s) 2472
BcnI CCSGG 2 cut(s) 478, 1553
BfaI CTAG 7 cut(s) 858, 1818, 2037, 2172, 2274, 2292, 2963
BfmI CTRYAG 1 cut(s) 2588
BfoI RGCGCY 2 cut(s) 1199, 2116
BfuAI ACCTGC 2 cut(s) 2998, 3364
BfuI GTATCC 1 cut(s) 775
BglII AGATCT 2 cut(s) 169, 1801
BisI GCNGC 4 cut(s) 450, 1233, 1560, 3631
BlsI GCNGC 4 cut(s) 451, 1234, 1561, 3632
Bme1390I CCNGG 4 cut(s) 478, 777, 905, 1553
Bme18I GGWCC 2 cut(s) 2987, 3338
BmeRI GACNNNNNGTC 1 cut(s) 1336
BmgBI CACGTC 1 cut(s) 1961
BmgT120I GGNCC 4 cut(s) 631, 1077, 2987, 3338
BmiI GGNNCC 5 cut(s) 2121, 2543, 2649, 2667, 2988
BmrFI CCNGG 4 cut(s) 478, 777, 905, 1553
BmrI ACTGGG 1 cut(s) 1925
BmsI GCATC 8 cut(s) 1389, 1423, 1492, 1527, 1546, 1929, 2611, 2994
BmtI GCTAGC 2 cut(s) 2175, 2277
BmuI ACTGGG 1 cut(s) 1925
BpiI GAAGAC 3 cut(s) 318, 1377, 3521
BpmI CTGGAG 3 cut(s) 1170, 1700, 2993
Bpu10I CCTNAGC 2 cut(s) 2130, 2232
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 1955
BpuMI CCSGG 2 cut(s) 478, 1553
BsaAI YACGTR 2 cut(s) 1631, 2735
BsaBI GATNNNNATC 3 cut(s) 168, 421, 2439
BsaI GGTCTC 2 cut(s) 1853, 2766
BsaJI CCNNGG 5 cut(s) 306, 612, 696, 1023, 3281
BsaWI WCCGGW 1 cut(s) 2485
BsaXI ACNNNNNCTCC 5 cut(s) 2198, 2228, 3242, 3272, 3685
Bsc4I CCNNNNNNNGG 5 cut(s) 2004, 2538, 3127, 3393, 3484
Bse118I RCCGGY 1 cut(s) 1604
Bse1I ACTGG 4 cut(s) 1920, 2026, 2721, 2810
Bse21I CCTNAGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 3366
Bse8I GATNNNNATC 3 cut(s) 168, 421, 2439
BseBI CCWGG 2 cut(s) 777, 905
BseDI CCNNGG 5 cut(s) 306, 612, 696, 1023, 3281
BseGI GGATG 8 cut(s) 166, 538, 622, 1380, 1542, 1561, 2013, 2470
BseJI GATNNNNATC 3 cut(s) 168, 421, 2439
BseLI CCNNNNNNNGG 5 cut(s) 2004, 2538, 3127, 3393, 3484
BseMI GCAATG 1 cut(s) 3366
BseNI ACTGG 4 cut(s) 1920, 2026, 2721, 2810
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 4 cut(s) 436, 1244, 1571, 3617
BsgI GTGCAG 1 cut(s) 2745
Bsh1236I CGCG 1 cut(s) 1255
BshFI GGCC 6 cut(s) 5, 467, 632, 1079, 1627, 3697
BshNI GGYRCC 1 cut(s) 2541
BsiHKAI GWGCWC 1 cut(s) 718
BsiSI CCGG 5 cut(s) 477, 1240, 1552, 1605, 2486
BslFI GGGAC 3 cut(s) 197, 2727, 3000
BslI CCNNNNNNNGG 5 cut(s) 2004, 2538, 3127, 3393, 3484
BsmBI CGTCTC 1 cut(s) 874
BsmFI GGGAC 3 cut(s) 197, 2727, 3000
BsnI GGCC 6 cut(s) 5, 467, 632, 1079, 1627, 3697
Bso31I GGTCTC 2 cut(s) 1853, 2766
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 718
Bsp19I CCATGG 4 cut(s) 306, 612, 1023, 3281
Bsp68I TCGCGA 1 cut(s) 1255
BspACI CCGC 1 cut(s) 2996
BspANI GGCC 6 cut(s) 5, 467, 632, 1079, 1627, 3697
BspFNI CGCG 1 cut(s) 1255
BspHI TCATGA 1 cut(s) 2469
BspLI GGNNCC 5 cut(s) 2121, 2543, 2649, 2667, 2988
BspMI ACCTGC 2 cut(s) 2998, 3364
BspOI GCTAGC 2 cut(s) 2175, 2277
BspPI GGATC 6 cut(s) 41, 158, 846, 1418, 2642, 2655
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 2541
BspTNI GGTCTC 2 cut(s) 1853, 2766
BsrDI GCAATG 1 cut(s) 3366
BsrFI RCCGGY 1 cut(s) 1604
BsrI ACTGG 4 cut(s) 1920, 2026, 2721, 2810
BssAI RCCGGY 1 cut(s) 1604
BssECI CCNNGG 5 cut(s) 306, 612, 696, 1023, 3281
BssT1I CCWWGG 5 cut(s) 306, 612, 696, 1023, 3281
Bst2UI CCWGG 2 cut(s) 777, 905
Bst4CI ACNGT 7 cut(s) 1186, 2055, 2085, 2826, 2971, 3322, 3520
Bst6I CTCTTC 5 cut(s) 2691, 3065, 3422, 3606, 3655
BstBAI YACGTR 2 cut(s) 1631, 2735
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 8 cut(s) 200, 1502, 1606, 2173, 2275, 3000, 3148, 3505
BstDSI CCRYGG 4 cut(s) 306, 612, 1023, 3281
BstF5I GGATG 8 cut(s) 166, 538, 622, 1380, 1542, 1561, 2013, 2470
BstFNI CGCG 1 cut(s) 1255
BstH2I RGCGCY 2 cut(s) 1199, 2116
BstHHI GCGC 2 cut(s) 1198, 2115
BstMWI GCNNNNNNNGC 7 cut(s) 596, 1939, 2119, 2584, 2923, 2999, 3004
BstNI CCWGG 2 cut(s) 777, 905
BstNSI RCATGY 2 cut(s) 710, 3259
BstSCI CCNGG 4 cut(s) 476, 775, 903, 1551
BstSFI CTRYAG 1 cut(s) 2588
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 1 cut(s) 1255
BstV1I GCAGC 4 cut(s) 436, 1244, 1571, 3617
BstV2I GAAGAC 3 cut(s) 318, 1377, 3521
BstX2I RGATCY 5 cut(s) 46, 169, 851, 1801, 2647
BstXI CCANNNNNNTGG 2 cut(s) 776, 1699
BstYI RGATCY 5 cut(s) 46, 169, 851, 1801, 2647
Bsu36I CCTNAGG 1 cut(s) 558
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 6 cut(s) 5, 467, 632, 1079, 1627, 3697
BtgI CCRYGG 4 cut(s) 306, 612, 1023, 3281
BtgZI GCGATG 1 cut(s) 3017
BtrI CACGTC 1 cut(s) 1961
BtsCI GGATG 8 cut(s) 166, 538, 622, 1380, 1542, 1561, 2013, 2470
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 3 cut(s) 651, 3086, 3443
BtuMI TCGCGA 1 cut(s) 1255
BveI ACCTGC 2 cut(s) 2998, 3364
Cac8I GCNNGC 8 cut(s) 200, 1502, 1606, 2173, 2275, 3000, 3148, 3505
CaiI CAGNNNCTG 2 cut(s) 557, 2596
CciI TCATGA 1 cut(s) 2469
CfoI GCGC 2 cut(s) 1198, 2115
Cfr10I RCCGGY 1 cut(s) 1604
Cfr13I GGNCC 4 cut(s) 631, 1077, 2987, 3338
CseI GACGC 1 cut(s) 806
Csp6I GTAC 3 cut(s) 554, 3554, 3568
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviQI GTAC 3 cut(s) 554, 3554, 3568
DraI TTTAAA 1 cut(s) 456
DriI GACNNNNNGTC 1 cut(s) 1336
EaeI YGGCCR 2 cut(s) 465, 1625
Eam1104I CTCTTC 5 cut(s) 2691, 3065, 3422, 3606, 3655
Eam1105I GACNNNNNGTC 1 cut(s) 1336
EarI CTCTTC 5 cut(s) 2691, 3065, 3422, 3606, 3655
EciI GGCGGA 1 cut(s) 2985
Eco130I CCWWGG 5 cut(s) 306, 612, 696, 1023, 3281
Eco31I GGTCTC 2 cut(s) 1853, 2766
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 2 cut(s) 2987, 3338
Eco47III AGCGCT 1 cut(s) 2114
Eco57I CTGAAG 4 cut(s) 27, 1602, 2618, 3284
Eco81I CCTNAGG 1 cut(s) 558
EcoO109I RGGNCCY 2 cut(s) 631, 2987
EcoRI GAATTC 4 cut(s) 945, 2040, 3097, 3454
EcoRII CCWGG 2 cut(s) 775, 903
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 5 cut(s) 306, 612, 696, 1023, 3281
EcoT22I ATGCAT 2 cut(s) 1298, 1542
ErhI CCWWGG 5 cut(s) 306, 612, 696, 1023, 3281
Esp3I CGTCTC 1 cut(s) 874
FaqI GGGAC 3 cut(s) 197, 2727, 3000
FauNDI CATATG 3 cut(s) 2978, 3329, 3689
FbaI TGATCA 1 cut(s) 2472
FblI GTMKAC 2 cut(s) 666, 1165
Fnu4HI GCNGC 4 cut(s) 450, 1233, 1560, 3631
FokI GGATG 8 cut(s) 173, 545, 629, 1367, 1549, 1568, 2020, 2477
Fsp4HI GCNGC 4 cut(s) 450, 1233, 1560, 3631
FspBI CTAG 7 cut(s) 858, 1818, 2037, 2172, 2274, 2292, 2963
GlaI GCGC 2 cut(s) 1197, 2114
GluI GCNGC 4 cut(s) 450, 1233, 1560, 3631
GsuI CTGGAG 3 cut(s) 1170, 1700, 2993
HaeII RGCGCY 2 cut(s) 1199, 2116
HaeIII GGCC 6 cut(s) 5, 467, 632, 1079, 1627, 3697
HapII CCGG 5 cut(s) 477, 1240, 1552, 1605, 2486
HgaI GACGC 1 cut(s) 806
HhaI GCGC 2 cut(s) 1198, 2115
Hin6I GCGC 2 cut(s) 1196, 2113
HinP1I GCGC 2 cut(s) 1196, 2113
HincII GTYRAC 3 cut(s) 91, 520, 2829
HindII GTYRAC 3 cut(s) 91, 520, 2829
HindIII AAGCTT 2 cut(s) 387, 1931
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 5 cut(s) 477, 1240, 1552, 1605, 2486
HphI GGTGA 5 cut(s) 445, 985, 2036, 3055, 3412
HpyCH4III ACNGT 7 cut(s) 1186, 2055, 2085, 2826, 2971, 3322, 3520
HpyCH4IV ACGT 3 cut(s) 1630, 1960, 2734
HpyF10VI GCNNNNNNNGC 7 cut(s) 596, 1939, 2119, 2584, 2923, 2999, 3004
HpySE526I ACGT 3 cut(s) 1630, 1960, 2734
HspAI GCGC 2 cut(s) 1196, 2113
KroI GCCGGC 1 cut(s) 1604
KroNI GCCGGC 1 cut(s) 1606
Ksp22I TGATCA 1 cut(s) 2472
KspAI GTTAAC 1 cut(s) 520
LmnI GCTCC 8 cut(s) 17, 1684, 2110, 2119, 2221, 2998, 3012, 3178
Lsp1109I GCAGC 4 cut(s) 436, 1244, 1571, 3617
LweI GCATC 8 cut(s) 1389, 1423, 1492, 1527, 1546, 1929, 2611, 2994
MaeI CTAG 7 cut(s) 858, 1818, 2037, 2172, 2274, 2292, 2963
MaeII ACGT 3 cut(s) 1630, 1960, 2734
MfeI CAATTG 3 cut(s) 213, 234, 564
MflI RGATCY 5 cut(s) 46, 169, 851, 1801, 2647
MhlI GDGCHC 1 cut(s) 718
MlsI TGGCCA 2 cut(s) 467, 1627
MluNI TGGCCA 2 cut(s) 467, 1627
MlyI GAGTC 6 cut(s) 123, 496, 578, 1121, 2528, 3342
MmeI TCCRAC 7 cut(s) 35, 1992, 2556, 3027, 3108, 3156, 3465
Mox20I TGGCCA 2 cut(s) 467, 1627
Mph1103I ATGCAT 2 cut(s) 1298, 1542
MroNI GCCGGC 1 cut(s) 1604
MroXI GAANNNNTTC 1 cut(s) 789
MscI TGGCCA 2 cut(s) 467, 1627
MslI CAYNNNNRTG 3 cut(s) 711, 2564, 2642
Msp20I TGGCCA 2 cut(s) 467, 1627
MspI CCGG 5 cut(s) 477, 1240, 1552, 1605, 2486
MspR9I CCNGG 4 cut(s) 478, 777, 905, 1553
MunI CAATTG 3 cut(s) 213, 234, 564
MvaI CCWGG 2 cut(s) 777, 905
MvnI CGCG 1 cut(s) 1255
MwoI GCNNNNNNNGC 7 cut(s) 596, 1939, 2119, 2584, 2923, 2999, 3004
NaeI GCCGGC 1 cut(s) 1606
NciI CCSGG 2 cut(s) 478, 1553
NcoI CCATGG 4 cut(s) 306, 612, 1023, 3281
NdeI CATATG 3 cut(s) 2978, 3329, 3689
NgoMIV GCCGGC 1 cut(s) 1604
NheI GCTAGC 2 cut(s) 2171, 2273
NlaIV GGNNCC 5 cut(s) 2121, 2543, 2649, 2667, 2988
NmuCI GTSAC 6 cut(s) 649, 748, 1180, 1886, 2477, 2498
NruI TCGCGA 1 cut(s) 1255
NsiI ATGCAT 2 cut(s) 1298, 1542
NspI RCATGY 2 cut(s) 710, 3259
NspV TTCGAA 1 cut(s) 381
OliI CACNNNNGTG 1 cut(s) 2642
PagI TCATGA 1 cut(s) 2469
PaqCI CACCTGC 1 cut(s) 3364
PciI ACATGT 2 cut(s) 706, 3255
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdiI GCCGGC 1 cut(s) 1606
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 8 cut(s) 422, 436, 892, 1070, 1469, 2570, 3013, 3370
PflFI GACNNNGTC 2 cut(s) 647, 2442
PflMI CCANNNNNTGG 1 cut(s) 3393
PfoI TCCNGGA 2 cut(s) 903, 1551
PkrI GCNGC 4 cut(s) 451, 1234, 1561, 3632
PleI GAGTC 6 cut(s) 123, 496, 577, 1120, 2528, 3342
PpsI GAGTC 6 cut(s) 123, 496, 577, 1120, 2528, 3342
Ppu21I YACGTR 2 cut(s) 1631, 2735
PpuMI RGGWCCY 1 cut(s) 2987
PscI ACATGT 2 cut(s) 706, 3255
PshBI ATTAAT 1 cut(s) 2864
PsiI TTATAA 2 cut(s) 498, 899
Psp5II RGGWCCY 1 cut(s) 2987
Psp6I CCWGG 2 cut(s) 775, 903
PspGI CCWGG 2 cut(s) 775, 903
PspN4I GGNNCC 5 cut(s) 2121, 2543, 2649, 2667, 2988
PspPI GGNCC 4 cut(s) 631, 1077, 2987, 3338
PspPPI RGGWCCY 1 cut(s) 2987
PstNI CAGNNNCTG 2 cut(s) 557, 2596
PsuI RGATCY 5 cut(s) 46, 169, 851, 1801, 2647
PsyI GACNNNGTC 2 cut(s) 647, 2442
RruI TCGCGA 1 cut(s) 1255
RsaI GTAC 3 cut(s) 555, 3555, 3569
RsaNI GTAC 3 cut(s) 554, 3554, 3568
RseI CAYNNNNRTG 3 cut(s) 711, 2564, 2642
SatI GCNGC 4 cut(s) 450, 1233, 1560, 3631
Sau96I GGNCC 4 cut(s) 631, 1077, 2987, 3338
SchI GAGTC 6 cut(s) 123, 496, 578, 1121, 2528, 3342
ScrFI CCNGG 4 cut(s) 478, 777, 905, 1553
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 8 cut(s) 1389, 1423, 1492, 1527, 1546, 1929, 2611, 2994
SfcI CTRYAG 1 cut(s) 2588
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 2 cut(s) 2987, 3338
SmiMI CAYNNNNRTG 3 cut(s) 711, 2564, 2642
SmlI CTYRAG 1 cut(s) 1934
SmoI CTYRAG 1 cut(s) 1934
SsiI CCGC 1 cut(s) 2996
SspI AATATT 2 cut(s) 1493, 2795
SspMI CTAG 7 cut(s) 858, 1818, 2037, 2172, 2274, 2292, 2963
StyD4I CCNGG 4 cut(s) 476, 775, 903, 1551
StyI CCWWGG 5 cut(s) 306, 612, 696, 1023, 3281
TaaI ACNGT 7 cut(s) 1186, 2055, 2085, 2826, 2971, 3322, 3520
TaiI ACGT 3 cut(s) 1633, 1963, 2737
TatI WGTACW 1 cut(s) 3567
TfiI GAWTC 8 cut(s) 422, 436, 892, 1070, 1469, 2570, 3013, 3370
TscAI CASTG 3 cut(s) 658, 3086, 3443
TseFI GTSAC 6 cut(s) 649, 748, 1180, 1886, 2477, 2498
TseI GCWGC 4 cut(s) 449, 1232, 1559, 3630
Tsp45I GTSAC 6 cut(s) 649, 748, 1180, 1886, 2477, 2498
TspGWI ACGGA 4 cut(s) 1462, 2654, 2894, 3209
TspRI CASTG 3 cut(s) 658, 3086, 3443
Tth111I GACNNNGTC 2 cut(s) 647, 2442
Van91I CCANNNNNTGG 1 cut(s) 3393
VneI GTGCAC 1 cut(s) 714
VpaK11BI GGWCC 2 cut(s) 2987, 3338
VspI ATTAAT 1 cut(s) 2864
XbaI TCTAGA 1 cut(s) 2036
XceI RCATGY 2 cut(s) 710, 3259
XcmI CCANNNNNNNNNTGG 2 cut(s) 3043, 3288
XmiI GTMKAC 2 cut(s) 666, 1165
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 7 cut(s) 858, 1818, 2037, 2172, 2274, 2292, 2963
Zsp2I ATGCAT 2 cut(s) 1298, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.