Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0004739

Physical Location & Seq

Genomic Coordinates Forward (+)

38213920 .. 38220459

Gene Structure 6540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g46040.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

ATGGCATTAATAAAAAGAAAGCCTCTGCACAACCTTGCTGAAACAGTTCATTCCCTGCTAGGACAAAAGTCACATCTTACTTCCAGTTGGGTTAAATCTGTTTGTCATATAATAATAAACCTGCCTTCTGAAGAGCAATCAATCGACTTAAGAACCACAGATTTAGACACAGTGAGTACTTCTGAGGATCCTGATAACGATTTAGGCAATCGTACCTCAAAGATTACAGATGAACTTGCTGTTTTGACTGCCCGGATAAATGAATTGAACAGACAGCGAAAGCAGCTTCTAAATGAATTTCTGGATTTGAAAGGTAATATTCGAGTGTTATGTCGTATAAGACCCATTGAAATGGGGGAGAACTTCGGCCGTTTTAGGCCTGTTGTAGCTTTGGATTCAAGTAATGTTCTTCTAAGATTTTCTGATAATAAGAACAAAAATTATACATTCGATAAGGTTTTCCACCACGGCTCATCACAAGATGAAGTCTTTTCAGAAGTTGAACCAGTAATCAAATCAGCACTGGATGGCTATAACGCTTGCATCTTTGCATACGGACAGACAGGGACAGGGAAAACCTTTACCATGGAAGGAATTCCAGAATCTCCTGGAGTGGTGCCCCGTGCAATTGAAGCACTGTTTAAGCAAGCAATGGATAGCAACCATGTGCATCTCTTTAGCTTCAGTATGCTAGAGATTTACATGGGGAATCTAAGAGACCTGCTAATCCCTCAACCAAGAAAGCCAATGGATCCAATGCCATGCCTCTTGATCCACACAGATCCAAAGGGTGAGATTGAAATTGAAAATCTTGTGGCTATTCGAGTGAGTGACTTGACCCAAGCTCTAAAGCTATACCGATTAGGGTGCCGGTTCAGGTCAACAGCATTGACAAATTGTAACATTACCTCTAGCAGATCCCACTGCTTGATTCGCATATCAATCACATGTAGCGGAACGCCTGAGAGGCGAAGGGAAACAAATAAAATTTGGTTTGTCGATCTTGGGGGAAGTGAGCGTTTGCTGAAGACAAAGGCATCCGGGAGAAGACTAGAAGAAGGAAAAGCCATTAACTTGTCACTTTCCTCTCTGGGAGATGTCATCAATGCCCTTCAACTCAGAAAGGGTCATGTACCTTATAGGAACAGCAAGCTGACACAAGTTCTGAAGGACTCTCTTGGAAGGGACTCAAAAACTCTGATGCTTGTCCATGTAAGTCCTAAAGAAGAAGATCTCTGTGAAACAGTATGTTCTTTGAACTTTGCAACAAGAGCGAGAAGTATTCATCTAGGGAATACAGACTCAACTGAACAAAGGGAGAAGGAAGTTGCAATGTTGAATCTGCAAGAAATGATGAACAAGATTGAAGTTGAGAGCCAGGATGTTAGGATGAGTATCAAGCAGTCAACTCAGGAGCTTCAAAATCTAAGGAAGACAGCTGCCTGTTCCACTGAGCAACTGGATAATTCAGTGGATGCATACCAGTTAGTGAACGAAGTGCCCCAGTCTAATCTTGAAACAAACAGGAAAAACACTGGATATATCTCAGCACCTCCCTTGTCCCAGGTACCAAGGTTTATGAGACCTACTGTTTGCAGCAGAAGAAAATCCGGATCAGACCAGCTCCTAAGTACTGATGAGAAACTGAGATTCCCTGCAAGAAGGAGAAGACCATTCAATTCCTATGCTAAATCTGTGAATTTTCCTGTAAAGAGTATTGCGGGAAACAATTCAGAAGGCAGTATTTCCAGAAATAGCTGTTCATTGCCTTTGACTGTGAACAACAGTGCAGATGTTGAGACAAATTACAGTCAAGATATATCAGAATATGATATTAAAGAGTTTGTTTCCTCAGACCAGGTACCATCACCAAAATGCTTGAATGAGCACAAATATCACCTCTTTGATGGTGAGGGAAGTGAAAAGGGAGATAAAAACAATGCTTGCTCTTCAAAACATTTGGAGGTTGATAAGTGGTTATGCCAGAAGAATGCATCAGCTATCAGGAGTCATGTTCATAGGAACAAAAGAGTTCTAGATATTCCTACCCCAGAGAAGAAACTAATGTGTAAAGGACACAAAGAAACAGAAAATTTCCAGGATGAGAAAGTTAATAATTACAAGTTTGCGGCGGAGCAGATTGTCAACCACAACAAATCGGAGAAACATGTGCATGTGGATTGTTCTCAAATGTTCATGCACGAAATGATTATTAGCAAGCCTCCTGCAAATATGATTGTCAATGACAATATGGACACAAAATGTAACTCTGATTTTTCATTGGATGGAATATTTAAGGAGACAAGGAAAAATATAAAAGACAAACTAGATGATGTGCTGATAGAGAAACACATACTCAGCAGTATCTATTCACCTGATATTTGGTGCAGCAATCTCAATTCGAATCAAGATGATAATGGAGTCGACTGTTTATCCACAATGCAGGCAGTATTTGGTGAAGCAGAATGCTCAGACAGTTCCTTATCTAACAACAGCAATTGGTGTCAAATTTCTAACGTTGATTTAGCTTCTAGTATTTTGGACTCGACACAAGATTCTAATATCTCAATTTCAAGAATTGAACAAAAGTCAGGCTGCCAACAGTTGCCTACCGCAACAGGTGTGGACAATAGTGAAAAGGAAGACCTAGAAAAATTATCTCAGAGGTCTGGAGAAGGTACAATGCCTGGCCAACAAAAAGTGAGGTATCAAAAAGCTTTGCTAATGAAAATTGAAATCCCTGAGGAACCAGTCAAGCCACAACAGCGGACAAAATCACAAGAAAATGATGTGCACTTAGGAACGTGCCGGCTTCTTCAACAGAAAATTCAGATATTATGGGCCAGTGCTCTTCTAGGATTAGGATTTCATGACTTGGGATTTGAGCAGGAATTCTTCCTTGGTTTAATGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

972

Amino Acids

109.42

Weight (kDa)

6.72

Isoelectric Point (pI)

51.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	31 - 183	3.6e-36	Microtubule binding
Kinesin	PF00225	80 - 369	1.1e-95	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55550
fragaria_vesca	FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040
malus_domestica	MD03G1001300.v1.1
prunus_persica	Prupe.6G000800_v2.0.a1
pyrus_communis	pycom03g00110
rosa_chinensis	RchiOBHm_Chr5g0084121
rosa_laevigata	RLG00000037083
rosa_multiflora	Rmu_ssc0000372.1_g000094
rosa_roxburghii	Rroxscaffold_1G00000520
rosa_rugosa	Rorug05G0489200 Rorug05G0489200 Rorug05G0489200 Rorug05G0489300
rosa_samantha	Rh5AG541800 Rh5BG567400 Rh5CG589200 Rh5DG575600
rosa_wichuraiana	Rw5G050400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	129, 729
Acc65I	GGTACC	2 cut(s)	1567, 1861
AccB1I	GGYRCC	4 cut(s)	616, 867, 1567, 1861
AccI	GTMKAC	1 cut(s)	2424
AccIII	TCCGGA	1 cut(s)	1610
AciI	CCGC	6 cut(s)	954, 1721, 2129, 2132, 2613, 2767
AclI	AACGTT	1 cut(s)	2517
AclWI	GGATC	8 cut(s)	182, 195, 746, 759, 766, 776, 912, 1621
AcoI	YGGCCR	2 cut(s)	367, 2689
AcsI	RAATTY	8 cut(s)	296, 594, 987, 1699, 2092, 2508, 2826, 2891
AcuI	CTGAAG	4 cut(s)	150, 667, 1046, 1187
AfaI	GTAC	7 cut(s)	178, 214, 1134, 1569, 1633, 1863, 2680
AflII	CTTAAG	1 cut(s)	148
AflIII	ACRYGT	2 cut(s)	947, 2167
AjnI	CCWGG	6 cut(s)	607, 1377, 1563, 1857, 2097, 2686
AjuI	GAANNNNNNNTTGG	2 cut(s)	2883, 2915
Alw21I	GWGCWC	3 cut(s)	1890, 2796, 2851
Alw26I	GTCTC	4 cut(s)	711, 1576, 1793, 2294
Alw44I	GTGCAC	1 cut(s)	2792
AlwI	GGATC	8 cut(s)	182, 195, 746, 759, 766, 776, 912, 1621
Aor13HI	TCCGGA	1 cut(s)	1610
AoxI	GGCC	4 cut(s)	367, 377, 2689, 2841
ApaLI	GTGCAC	1 cut(s)	2792
ApeKI	GCWGC	5 cut(s)	283, 1439, 1596, 2388, 2595
ApoI	RAATTY	8 cut(s)	296, 594, 987, 1699, 2092, 2508, 2826, 2891
ArsI	GACNNNNNNTTYG	4 cut(s)	1575, 1607, 2864, 2896
AseI	ATTAAT	1 cut(s)	8
Asp700I	GAANNNNTTC	3 cut(s)	45, 594, 1729
Asp718I	GGTACC	2 cut(s)	1567, 1861
AspS9I	GGNCC	1 cut(s)	2841
AsuC2I	CCSGG	2 cut(s)	253, 1042
AsuHPI	GGTGA	6 cut(s)	803, 1860, 1889, 1922, 2364, 2468
AsuII	TTCGAA	1 cut(s)	2402
AxyI	CCTNAGG	1 cut(s)	2742
BaeGI	GKGCMC	3 cut(s)	621, 1503, 2796
BaeI	ACNNNNGTAYC	2 cut(s)	1559, 1592
BalI	TGGCCA	1 cut(s)	2691
BamHI	GGATCC	2 cut(s)	187, 751
BanI	GGYRCC	4 cut(s)	616, 867, 1567, 1861
BbsI	GAAGAC	5 cut(s)	1034, 1054, 1439, 1675, 2649
Bbv12I	GWGCWC	3 cut(s)	1890, 2796, 2851
BbvI	GCAGC	5 cut(s)	295, 1426, 1608, 2400, 2582
BccI	CCATC	4 cut(s)	521, 1873, 1901, 2279
BceAI	ACGGC	2 cut(s)	354, 484
BcgI	CGANNNNNNTGC	2 cut(s)	849, 883
BciT130I	CCWGG	6 cut(s)	609, 1379, 1565, 1859, 2099, 2688
BcnI	CCSGG	2 cut(s)	253, 1042
BcoDI	GTCTC	4 cut(s)	711, 1576, 1793, 2294
BfrI	CTTAAG	1 cut(s)	148
BfuAI	ACCTGC	2 cut(s)	129, 729
BglI	GCCNNNNNGGC	1 cut(s)	967
BglII	AGATCT	1 cut(s)	1231
BisI	GCNGC	6 cut(s)	284, 1440, 1597, 2130, 2389, 2596
BlsI	GCNGC	6 cut(s)	285, 1441, 1598, 2131, 2390, 2597
BmcAI	AGTACT	2 cut(s)	178, 1633
Bme1390I	CCNGG	8 cut(s)	253, 609, 1042, 1379, 1565, 1859, 2099, 2688
BmgT120I	GGNCC	1 cut(s)	2841
BmiI	GGNNCC	7 cut(s)	189, 618, 753, 869, 1569, 1863, 2748
BmrFI	CCNGG	8 cut(s)	253, 609, 1042, 1379, 1565, 1859, 2099, 2688
BmrI	ACTGGG	1 cut(s)	1498
BmsI	GCATC	6 cut(s)	552, 679, 1046, 1191, 1465, 2003
BmuI	ACTGGG	1 cut(s)	1498
BoxI	GACNNNNGTC	1 cut(s)	67
BpiI	GAAGAC	5 cut(s)	1034, 1054, 1439, 1675, 2649
BpmI	CTGGAG	2 cut(s)	630, 2691
Bpu14I	TTCGAA	1 cut(s)	2402
BpuMI	CCSGG	2 cut(s)	253, 1042
BsaBI	GATNNNNATC	1 cut(s)	1394
BsaI	GGTCTC	2 cut(s)	711, 1576
BsaJI	CCNNGG	5 cut(s)	466, 585, 1563, 1571, 2899
BsaWI	WCCGGW	1 cut(s)	1610
Bse118I	RCCGGY	2 cut(s)	870, 2808
Bse1I	ACTGG	9 cut(s)	84, 506, 528, 1464, 1483, 1504, 1540, 2750, 2844
Bse21I	CCTNAGG	1 cut(s)	2742
Bse3DI	GCAATG	3 cut(s)	657, 1338, 1763
Bse8I	GATNNNNATC	1 cut(s)	1394
BseAI	TCCGGA	1 cut(s)	1610
BseBI	CCWGG	6 cut(s)	609, 1379, 1565, 1859, 2099, 2688
BseDI	CCNNGG	5 cut(s)	466, 585, 1563, 1571, 2899
BseGI	GGATG	7 cut(s)	532, 1037, 1387, 1395, 1480, 2107, 2290
BseJI	GATNNNNATC	1 cut(s)	1394
BseMI	GCAATG	3 cut(s)	657, 1338, 1763
BseNI	ACTGG	9 cut(s)	84, 506, 528, 1464, 1483, 1504, 1540, 2750, 2844
BseSI	GKGCMC	3 cut(s)	621, 1503, 2796
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	5 cut(s)	295, 1426, 1608, 2400, 2582
BsgI	GTGCAG	3 cut(s)	11, 1809, 2407
Bsh1285I	CGRYCG	1 cut(s)	370
BshFI	GGCC	4 cut(s)	369, 379, 2691, 2843
BshNI	GGYRCC	4 cut(s)	616, 867, 1567, 1861
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	3 cut(s)	1890, 2796, 2851
BsiSI	CCGG	5 cut(s)	253, 871, 1041, 1611, 2809
BslFI	GGGAC	3 cut(s)	580, 1199, 1546
BsmAI	GTCTC	4 cut(s)	711, 1576, 1793, 2294
BsmFI	GGGAC	3 cut(s)	580, 1199, 1546
BsmI	GAATGC	2 cut(s)	1996, 2471
BsnI	GGCC	4 cut(s)	369, 379, 2691, 2843
Bso31I	GGTCTC	2 cut(s)	711, 1576
Bsp119I	TTCGAA	1 cut(s)	2402
Bsp1286I	GDGCHC	5 cut(s)	621, 1503, 1890, 2796, 2851
Bsp13I	TCCGGA	1 cut(s)	1610
Bsp143I	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
Bsp19I	CCATGG	1 cut(s)	585
BspACI	CCGC	6 cut(s)	954, 1721, 2129, 2132, 2613, 2767
BspANI	GGCC	4 cut(s)	369, 379, 2691, 2843
BspEI	TCCGGA	1 cut(s)	1610
BspHI	TCATGA	1 cut(s)	2869
BspLI	GGNNCC	7 cut(s)	189, 618, 753, 869, 1569, 1863, 2748
BspMI	ACCTGC	2 cut(s)	129, 729
BspPI	GGATC	8 cut(s)	182, 195, 746, 759, 766, 776, 912, 1621
BspQI	GCTCTTC	3 cut(s)	126, 1954, 2856
BspT104I	TTCGAA	1 cut(s)	2402
BspT107I	GGYRCC	4 cut(s)	616, 867, 1567, 1861
BspTI	CTTAAG	1 cut(s)	148
BspTNI	GGTCTC	2 cut(s)	711, 1576
BsrDI	GCAATG	3 cut(s)	657, 1338, 1763
BsrFI	RCCGGY	2 cut(s)	870, 2808
BsrI	ACTGG	9 cut(s)	84, 506, 528, 1464, 1483, 1504, 1540, 2750, 2844
BssAI	RCCGGY	2 cut(s)	870, 2808
BssECI	CCNNGG	5 cut(s)	466, 585, 1563, 1571, 2899
BssMI	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
BssT1I	CCWWGG	3 cut(s)	585, 1571, 2899
Bst2UI	CCWGG	6 cut(s)	609, 1379, 1565, 1859, 2099, 2688
Bst6I	CTCTTC	3 cut(s)	126, 1954, 2856
BstAFI	CTTAAG	1 cut(s)	148
BstBI	TTCGAA	1 cut(s)	2402
BstC8I	GCNNGC	7 cut(s)	541, 648, 1151, 1945, 2219, 2445, 2810
BstDSI	CCRYGG	2 cut(s)	466, 585
BstF5I	GGATG	7 cut(s)	532, 1037, 1387, 1395, 1480, 2107, 2290
BstKTI	GATC	8 cut(s)	190, 754, 774, 784, 920, 1003, 1234, 1616
BstMAI	GTCTC	4 cut(s)	711, 1576, 1793, 2294
BstMBI	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
BstMCI	CGRYCG	1 cut(s)	370
BstMWI	GCNNNNNNNGC	6 cut(s)	283, 632, 933, 967, 1271, 2764
BstNI	CCWGG	6 cut(s)	609, 1379, 1565, 1859, 2099, 2688
BstNSI	RCATGY	3 cut(s)	951, 2171, 2177
BstPAI	GACNNNNGTC	1 cut(s)	67
BstSCI	CCNGG	8 cut(s)	251, 607, 1040, 1377, 1563, 1857, 2097, 2686
BstSLI	GKGCMC	3 cut(s)	621, 1503, 2796
BstV1I	GCAGC	5 cut(s)	295, 1426, 1608, 2400, 2582
BstV2I	GAAGAC	5 cut(s)	1034, 1054, 1439, 1675, 2649
BstX2I	RGATCY	5 cut(s)	187, 751, 781, 917, 1231
BstXI	CCANNNNNNTGG	1 cut(s)	352
BstYI	RGATCY	5 cut(s)	187, 751, 781, 917, 1231
BstZI	CGGCCG	1 cut(s)	367
Bsu36I	CCTNAGG	1 cut(s)	2742
BsuRI	GGCC	4 cut(s)	369, 379, 2691, 2843
BtgI	CCRYGG	2 cut(s)	466, 585
BtsCI	GGATG	7 cut(s)	532, 1037, 1387, 1395, 1480, 2107, 2290
BtsI	GCAGTG	1 cut(s)	922
BtsIMutI	CAGTG	9 cut(s)	177, 521, 635, 922, 1449, 1476, 1533, 1792, 2851
BveI	ACCTGC	2 cut(s)	129, 729
Cac8I	GCNNGC	7 cut(s)	541, 648, 1151, 1945, 2219, 2445, 2810
CciI	TCATGA	1 cut(s)	2869
Cfr10I	RCCGGY	2 cut(s)	870, 2808
Cfr13I	GGNCC	1 cut(s)	2841
CsiI	ACCWGGT	1 cut(s)	1857
Csp6I	GTAC	7 cut(s)	177, 213, 1133, 1568, 1632, 1862, 2679
CspCI	CAANNNNNGTGG	2 cut(s)	2604, 2639
CviQI	GTAC	7 cut(s)	177, 213, 1133, 1568, 1632, 1862, 2679
DpnI	GATC	8 cut(s)	189, 753, 773, 783, 919, 1002, 1233, 1615
DpnII	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
EaeI	YGGCCR	2 cut(s)	367, 2689
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	3 cut(s)	126, 1954, 2856
EarI	CTCTTC	3 cut(s)	126, 1954, 2856
EciI	GGCGGA	1 cut(s)	2147
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	3 cut(s)	585, 1571, 2899
Eco147I	AGGCCT	1 cut(s)	379
Eco31I	GGTCTC	2 cut(s)	711, 1576
Eco52I	CGGCCG	1 cut(s)	367
Eco57I	CTGAAG	4 cut(s)	150, 667, 1046, 1187
Eco81I	CCTNAGG	1 cut(s)	2742
EcoRI	GAATTC	2 cut(s)	594, 2891
EcoRII	CCWGG	6 cut(s)	607, 1377, 1563, 1857, 2097, 2686
EcoT14I	CCWWGG	3 cut(s)	585, 1571, 2899
EcoT22I	ATGCAT	2 cut(s)	1480, 1996
ErhI	CCWWGG	3 cut(s)	585, 1571, 2899
FaqI	GGGAC	3 cut(s)	580, 1199, 1546
FauI	CCCGC	1 cut(s)	1714
FblI	GTMKAC	1 cut(s)	2424
Fnu4HI	GCNGC	6 cut(s)	284, 1440, 1597, 2130, 2389, 2596
FokI	GGATG	7 cut(s)	539, 1024, 1394, 1402, 1487, 2114, 2297
Fsp4HI	GCNGC	6 cut(s)	284, 1440, 1597, 2130, 2389, 2596
GluI	GCNGC	6 cut(s)	284, 1440, 1597, 2130, 2389, 2596
GsuI	CTGGAG	2 cut(s)	630, 2691
HaeIII	GGCC	4 cut(s)	369, 379, 2691, 2843
HapII	CCGG	5 cut(s)	253, 871, 1041, 1611, 2809
HincII	GTYRAC	4 cut(s)	882, 1407, 2146, 2425
HindII	GTYRAC	4 cut(s)	882, 1407, 2146, 2425
HindIII	AAGCTT	1 cut(s)	2715
HpaII	CCGG	5 cut(s)	253, 871, 1041, 1611, 2809
HphI	GGTGA	6 cut(s)	803, 1860, 1889, 1922, 2364, 2468
Hpy166II	GTNNAC	8 cut(s)	882, 1407, 1492, 1780, 2146, 2425, 2626, 2794
Hpy8I	GTNNAC	8 cut(s)	882, 1407, 1492, 1780, 2146, 2425, 2626, 2794
HpyCH4IV	ACGT	2 cut(s)	2517, 2804
HpyF10VI	GCNNNNNNNGC	6 cut(s)	283, 632, 933, 967, 1271, 2764
HpySE526I	ACGT	2 cut(s)	2517, 2804
Kpn2I	TCCGGA	1 cut(s)	1610
KpnI	GGTACC	2 cut(s)	1571, 1865
KroI	GCCGGC	1 cut(s)	2808
KroNI	GCCGGC	1 cut(s)	2810
Kzo9I	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
LguI	GCTCTTC	3 cut(s)	126, 1954, 2856
LmnI	GCTCC	3 cut(s)	1414, 1629, 2134
Lsp1109I	GCAGC	5 cut(s)	295, 1426, 1608, 2400, 2582
LweI	GCATC	6 cut(s)	552, 679, 1046, 1191, 1465, 2003
MabI	ACCWGGT	1 cut(s)	1857
MaeII	ACGT	2 cut(s)	2517, 2804
MaeIII	GTNAC	5 cut(s)	69, 830, 899, 1077, 2264
MalI	GATC	8 cut(s)	189, 753, 773, 783, 919, 1002, 1233, 1615
MboI	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
MfeI	CAATTG	2 cut(s)	627, 2497
MflI	RGATCY	5 cut(s)	187, 751, 781, 917, 1231
MhlI	GDGCHC	5 cut(s)	621, 1503, 1890, 2796, 2851
MlsI	TGGCCA	1 cut(s)	2691
MluNI	TGGCCA	1 cut(s)	2691
MlyI	GAGTC	6 cut(s)	1166, 1181, 1295, 2017, 2430, 2537
Mox20I	TGGCCA	1 cut(s)	2691
Mph1103I	ATGCAT	2 cut(s)	1480, 1996
MroI	TCCGGA	1 cut(s)	1610
MroNI	GCCGGC	1 cut(s)	2808
MroXI	GAANNNNTTC	3 cut(s)	45, 594, 1729
MscI	TGGCCA	1 cut(s)	2691
MseI	TTAA	9 cut(s)	8, 93, 149, 642, 1071, 1836, 2112, 2295, 2906
MslI	CAYNNNNRTG	3 cut(s)	350, 1873, 2172
Msp20I	TGGCCA	1 cut(s)	2691
MspA1I	CMGCKG	2 cut(s)	1439, 2767
MspCI	CTTAAG	1 cut(s)	148
MspI	CCGG	5 cut(s)	253, 871, 1041, 1611, 2809
MspR9I	CCNGG	8 cut(s)	253, 609, 1042, 1379, 1565, 1859, 2099, 2688
MunI	CAATTG	2 cut(s)	627, 2497
Mva1269I	GAATGC	2 cut(s)	1996, 2471
MvaI	CCWGG	6 cut(s)	609, 1379, 1565, 1859, 2099, 2688
MwoI	GCNNNNNNNGC	6 cut(s)	283, 632, 933, 967, 1271, 2764
NaeI	GCCGGC	1 cut(s)	2810
NciI	CCSGG	2 cut(s)	253, 1042
NcoI	CCATGG	1 cut(s)	585
NdeII	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
NgoMIV	GCCGGC	1 cut(s)	2808
NlaIV	GGNNCC	7 cut(s)	189, 618, 753, 869, 1569, 1863, 2748
NmuCI	GTSAC	3 cut(s)	69, 830, 1077
NsiI	ATGCAT	2 cut(s)	1480, 1996
NspI	RCATGY	3 cut(s)	951, 2171, 2177
NspV	TTCGAA	1 cut(s)	2402
PagI	TCATGA	1 cut(s)	2869
PceI	AGGCCT	1 cut(s)	379
PciI	ACATGT	2 cut(s)	947, 2167
PciSI	GCTCTTC	3 cut(s)	126, 1954, 2856
PctI	GAATGC	2 cut(s)	1996, 2471
PdiI	GCCGGC	1 cut(s)	2810
PdmI	GAANNNNTTC	3 cut(s)	45, 594, 1729
PfeI	GAWTC	8 cut(s)	395, 602, 709, 931, 1339, 1650, 2404, 2555
PfoI	TCCNGGA	3 cut(s)	607, 1040, 2097
PkrI	GCNGC	6 cut(s)	285, 1441, 1598, 2131, 2390, 2597
PleI	GAGTC	6 cut(s)	1166, 1181, 1295, 2016, 2429, 2537
PpsI	GAGTC	6 cut(s)	1166, 1181, 1295, 2016, 2429, 2537
PscI	ACATGT	2 cut(s)	947, 2167
PshAI	GACNNNNGTC	1 cut(s)	67
PshBI	ATTAAT	1 cut(s)	8
Psp1406I	AACGTT	1 cut(s)	2517
Psp6I	CCWGG	6 cut(s)	607, 1377, 1563, 1857, 2097, 2686
PspGI	CCWGG	6 cut(s)	607, 1377, 1563, 1857, 2097, 2686
PspN4I	GGNNCC	7 cut(s)	189, 618, 753, 869, 1569, 1863, 2748
PspPI	GGNCC	1 cut(s)	2841
PsuI	RGATCY	5 cut(s)	187, 751, 781, 917, 1231
PvuII	CAGCTG	1 cut(s)	1439
RsaI	GTAC	7 cut(s)	178, 214, 1134, 1569, 1633, 1863, 2680
RsaNI	GTAC	7 cut(s)	177, 213, 1133, 1568, 1632, 1862, 2679
RseI	CAYNNNNRTG	3 cut(s)	350, 1873, 2172
SalI	GTCGAC	1 cut(s)	2423
SapI	GCTCTTC	3 cut(s)	126, 1954, 2856
SaqAI	TTAA	9 cut(s)	8, 93, 149, 642, 1071, 1836, 2112, 2295, 2906
SatI	GCNGC	6 cut(s)	284, 1440, 1597, 2130, 2389, 2596
Sau3AI	GATC	8 cut(s)	187, 751, 771, 781, 917, 1000, 1231, 1613
Sau96I	GGNCC	1 cut(s)	2841
ScaI	AGTACT	2 cut(s)	178, 1633
SchI	GAGTC	6 cut(s)	1166, 1181, 1295, 2017, 2430, 2537
ScrFI	CCNGG	8 cut(s)	253, 609, 1042, 1379, 1565, 1859, 2099, 2688
SduI	GDGCHC	5 cut(s)	621, 1503, 1890, 2796, 2851
SexAI	ACCWGGT	1 cut(s)	1857
SfaNI	GCATC	6 cut(s)	552, 679, 1046, 1191, 1465, 2003
SfuI	TTCGAA	1 cut(s)	2402
SmiMI	CAYNNNNRTG	3 cut(s)	350, 1873, 2172
SmlI	CTYRAG	1 cut(s)	148
SmoI	CTYRAG	1 cut(s)	148
SseBI	AGGCCT	1 cut(s)	379
SsiI	CCGC	6 cut(s)	954, 1721, 2129, 2132, 2613, 2767
SspI	AATATT	2 cut(s)	319, 2292
StuI	AGGCCT	1 cut(s)	379
StyD4I	CCNGG	8 cut(s)	251, 607, 1040, 1377, 1563, 1857, 2097, 2686
StyI	CCWWGG	3 cut(s)	585, 1571, 2899
TaiI	ACGT	2 cut(s)	2520, 2807
TaqI	TCGA	8 cut(s)	144, 322, 450, 823, 999, 2402, 2424, 2546
TatI	WGTACW	2 cut(s)	176, 1631
TauI	GCSGC	1 cut(s)	2132
TfiI	GAWTC	8 cut(s)	395, 602, 709, 931, 1339, 1650, 2404, 2555
Tru1I	TTAA	9 cut(s)	8, 93, 149, 642, 1071, 1836, 2112, 2295, 2906
Tru9I	TTAA	9 cut(s)	8, 93, 149, 642, 1071, 1836, 2112, 2295, 2906
TscAI	CASTG	9 cut(s)	177, 528, 642, 929, 1456, 1476, 1540, 1792, 2851
TseFI	GTSAC	3 cut(s)	69, 830, 1077
TseI	GCWGC	5 cut(s)	283, 1439, 1596, 2388, 2595
Tsp45I	GTSAC	3 cut(s)	69, 830, 1077
TspGWI	ACGGA	1 cut(s)	570
TspRI	CASTG	9 cut(s)	177, 528, 642, 929, 1456, 1476, 1540, 1792, 2851
Vha464I	CTTAAG	1 cut(s)	148
VneI	GTGCAC	1 cut(s)	2792
VspI	ATTAAT	1 cut(s)	8
XapI	RAATTY	8 cut(s)	296, 594, 987, 1699, 2092, 2508, 2826, 2891
XbaI	TCTAGA	1 cut(s)	2035
XceI	RCATGY	3 cut(s)	951, 2171, 2177
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1456
XmiI	GTMKAC	1 cut(s)	2424
XmnI	GAANNNNTTC	3 cut(s)	45, 594, 1729
ZrmI	AGTACT	2 cut(s)	178, 1633
Zsp2I	ATGCAT	2 cut(s)	1480, 1996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.