Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0004739

Physical Location & Seq

Genomic Coordinates Reverse (-)

971635 .. 980222

Gene Structure 8588 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00000520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGGCATCAAGAACAAGAAAGCCTCTCCACAACCTTGCCGAAACAATTCATTCCCTGCTAGGACAAAAGTCGCATCTTACTTCCAGTTGGGTTAAATCTGTTTGTCATATAATAATAAACCTACCTTCTGAAGAGCAATCAACCGACTTAAGAACCACAAATATAGAGACAGTGAGTGCTTCAGAGAATCCTGATGATGAATTAGGCAATCGTACCTCAAAGATTACAGATGAACTTGCTATCTTGACTGCCCGGATAAATGAATTGAACAGACAGCGAAAGCAGTTTCTAAATGAATTTCTGGATTTGAAAGGTAATATTCGAGTATTTTGTCGTATAAGACCCATTGAAATGGGGGAGAACTTTGGCCGTTTTAGGCCTGTTGTAGCTTTGGATTCAAGTACTGTTCTTCTAAGATTTTCGGATAATAAGAATAAAAGTTACAGTTTTGACAAGGTTTTCCACCCTGGCTCATCGCAAGATGAAGTCTTTTCAGAAGTTGAACCAGTTATCAAATCAGCACTGGATGGCTATAACGCTTGCATCTTTGCATATGGACAGACAGGGACAGGGAAAACCTTCACCATGGAAGGAATTCCAGAAGATCCTGGAATGGTGCCCCGTGCAATTGAAGCACTGTTTAAGCAAGCCACGGATAGCAACCATGTGTATCTCTTTAGTTTCAGTATGCTTGAGATTTACATGGGAAATCTGAGAGACCTGCTAATCTCTCAACCAACAAAGCCAATGGATCCAATGCCATGCCTGTCAATCCACACAGATCCTAAGGGTGAGATTGAAATTGAAAACCTTGTGGCAATTCGAGTGAGTGGCTTGACCCAAGCTATAAAACTATACAGATTAGGGTGCCGGTTCAGGTCAACTGCATCGACAAACTGTAACATTACCTCCAGCAGATCCCACTGGGGAAGTGAGCGTTTGCTGAAGACAAAGGCATCCGGGAGAAGACTAGAAGAAGGAAAAGCCATTAACTTGTCCCTTTCCTCTCTGGGAGATGTCATCAATGCCCTTCAACTCAGAAAGGGTCATGTACCTTATAGGAACAGCAAGCTGACACAAGTTCTTAAAGACTCCCTTGGAAGGGACTCAAAAACTCTGATGCTTGTCCATGCAAGTCCTAAAGAAGAAGATCTCTGTGAAACAGTATGTTCTTTGAACTTTGCAACAAGAGCGAGAACTATCCATCTGGGGAATACAGACTCCACTGAACAAAGGGAGAAGGAAGTTGCAATGATGAATCTGCAAGAAATGATGAACAAGATTGAAGTTGAGCGCCAGGATGTTAGGATGAGAATCAAGCAGTCAACTCAGGAACTTCAAATTCTAAGGAGTACAGCTGCCTGTTCCATTGAGCAACTGGATAACTCAGTGGATGCTTTCCAGTTAATGAATGAAGTGCCCCAACCTAATCTTGAAACAAACAGGAACAATACTGCATGTATCTCAGCACCTCCCTTATCCCAGGTACCAAGGTTTATGAGACCTACTGTTTGCAGCAGAAGAAAATCTGGATCAGACCACCTCCTAACTACTGAAGAGAAACTGGAATTCCCTGCAAGAAGGAGAAGACCATTCAATCATTATGCTAAATCCGTGAATTTCCCTGTAAAGGGTATTTCAGGAAACAATTCAGAAGGCAGTCTTTCCAGAAATAGCTGTTTAGTGCCTTTGACTGTGAACAAAAGTGCAGATGTTGAGACAAATTACAGTCAAGATCTATCAGAATGTGATATTAAAGAGGTTGTTTCCCCAGACCAGGTAACATCACCAAAATGCTTGAATCAGCACAGATATCACGTCGTTCATGGTGAGGGAAGTGAAAATGGAGATAAAAGTAATGCTTGCTCTGGAAAACATTTGAAGGTCGATAAGTGGCTATGCCAGAAGAATGCGCCTGCTAGCAGGAGTCATGTTTATAGGAACAAACGAGTTCTAGCTATTCCTACCCCAGAGAAGAAACTAATGTGTAAAGGACAAAAACAAACAGAAAAATTCCAGGATGAGAAAGTCAATAATTACAAGCTTGCTGTGGAACAGATTGTCAACCACAACAAATCGGGGAAACACGTGGATGTGAAGGAAATGTTCATGCAAGAAGTGATTATTAGCAAACCTCCCGCTAATATGAACGTCAATGACAATATTGACCCAAAATGTCACTCTGATTCTTTATCGGATGGAATAGTTAAAGAGACAATGATAAATGTACAATACAAACTAGATGACGAGCTGACAGAGAAACACATGCTTAGCAGTATCTATCCACCTGATATTTGGTGCTGCAATTTCAATTCAACTCAAGATGATAATGGAGTCAACTGTTTATCCACAAAGCAGGCAGTATATGGTGAAACAGAATGCTCAGACAGTTCCTTGTCTAACAGCAGCAATTGGTGTCAAATTTCTACCATTGTTTTAGCTGATAGTATTTGGGACGCAAGACAAGATTCTAATAACTCAATTTCAAGAATTGAACAAAAGTCAGGCTGCCAACGGTTGCCCATCGTAATAGGTGTGGATGATAGTGAGAAGGAAGACTTGGAAAAATTATCTCAGATCTCTGGAGAAGGTACATGCCTACAAAAAGTGAGATCTCAAAGAGCTTTATTAATGAAAAATGTAATTCTAGAGGAACCAGTCAAGCCACAACAACTGACAAGATCTCAAGACAATAATGTGCACCTAGGAATATGCCATCTTCTTCAACAGAAAATTCAGATATTATGGACCGGTGCTCTTCTAGGATTAGGATTTCAAAACTTGGGACTTGAGCAGGAATTCTTCCTTGGTTTAATGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

940

Amino Acids

105.65

Weight (kDa)

7.28

Isoelectric Point (pI)

51.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	90 - 242	1.9e-38	Microtubule binding
Kinesin	PF00225	140 - 308	4.3e-49	Kinesin motor domain
Kinesin	PF00225	310 - 401	1.2e-31	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55550
fragaria_vesca	FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040
malus_domestica	MD03G1001300.v1.1
prunus_persica	Prupe.6G000800_v2.0.a1
pyrus_communis	pycom03g00110
rosa_chinensis	RchiOBHm_Chr5g0084121
rosa_laevigata	RLG00000037083
rosa_multiflora	Rmu_ssc0000372.1_g000094
rosa_roxburghii	Rroxscaffold_1G00000520
rosa_rugosa	Rorug05G0489200 Rorug05G0489200 Rorug05G0489200 Rorug05G0489300
rosa_samantha	Rh5AG541800 Rh5BG567400 Rh5CG589200 Rh5DG575600
rosa_wichuraiana	Rw5G050400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	729
Acc65I	GGTACC	1 cut(s)	1486
AccB1I	GGYRCC	3 cut(s)	616, 867, 1486
AciI	CCGC	1 cut(s)	2139
AclWI	GGATC	6 cut(s)	599, 746, 759, 776, 912, 1540
AcoI	YGGCCR	1 cut(s)	367
AcsI	RAATTY	9 cut(s)	296, 594, 1341, 1568, 1618, 2012, 2421, 2733, 2798
AcuI	CTGAAG	4 cut(s)	150, 165, 965, 1575
AcvI	CACGTG	1 cut(s)	2089
AfaI	GTAC	7 cut(s)	214, 403, 1053, 1354, 1488, 2229, 2593
AfiI	CCNNNNNNNGG	5 cut(s)	1101, 1102, 1630, 1631, 1777
AflII	CTTAAG	1 cut(s)	148
AflIII	ACRYGT	1 cut(s)	2086
AgeI	ACCGGT	1 cut(s)	2750
AjiI	CACGTC	1 cut(s)	1819
AjnI	CCWGG	6 cut(s)	466, 607, 1296, 1482, 1776, 2016
AjuI	GAANNNNNNNTTGG	4 cut(s)	2543, 2575, 2790, 2822
Alw21I	GWGCWC	2 cut(s)	2703, 2758
Alw26I	GTCTC	5 cut(s)	161, 711, 1495, 1712, 2207
Alw44I	GTGCAC	1 cut(s)	2699
AlwI	GGATC	6 cut(s)	599, 746, 759, 776, 912, 1540
AoxI	GGCC	2 cut(s)	367, 377
ApaLI	GTGCAC	1 cut(s)	2699
ApeKI	GCWGC	5 cut(s)	1358, 1515, 2301, 2406, 2508
ApoI	RAATTY	9 cut(s)	296, 594, 1341, 1568, 1618, 2012, 2421, 2733, 2798
ArsI	GACNNNNNNTTYG	2 cut(s)	1494, 1526
AseI	ATTAAT	1 cut(s)	2630
AsiGI	ACCGGT	1 cut(s)	2750
Asp700I	GAANNNNTTC	4 cut(s)	45, 578, 594, 1648
Asp718I	GGTACC	1 cut(s)	1486
AspA2I	CCTAGG	1 cut(s)	2704
AspLEI	GCGC	2 cut(s)	1296, 1915
AspS9I	GGNCC	1 cut(s)	2748
AsuC2I	CCSGG	2 cut(s)	253, 961
AsuHPI	GGTGA	5 cut(s)	574, 803, 1779, 1841, 2381
AsuNHI	GCTAGC	1 cut(s)	1919
AvaII	GGWCC	1 cut(s)	2748
AvrII	CCTAGG	1 cut(s)	2704
AxyI	CCTNAGG	1 cut(s)	786
BaeGI	GKGCMC	3 cut(s)	621, 1422, 2703
BaeI	ACNNNNGTAYC	2 cut(s)	1478, 1511
BamHI	GGATCC	1 cut(s)	751
BanI	GGYRCC	3 cut(s)	616, 867, 1486
BbrPI	CACGTG	1 cut(s)	2089
BbsI	GAAGAC	4 cut(s)	953, 973, 1594, 2562
Bbv12I	GWGCWC	2 cut(s)	2703, 2758
BbvI	GCAGC	5 cut(s)	1345, 1527, 2288, 2418, 2495
BccI	CCATC	5 cut(s)	521, 1212, 2192, 2531, 2724
BceAI	ACGGC	1 cut(s)	354
BciT130I	CCWGG	6 cut(s)	468, 609, 1298, 1484, 1778, 2018
BcnI	CCSGG	2 cut(s)	253, 961
BcoDI	GTCTC	5 cut(s)	161, 711, 1495, 1712, 2207
BfaI	CTAG	8 cut(s)	59, 971, 1920, 1955, 2240, 2648, 2705, 2762
BfoI	RGCGCY	1 cut(s)	1297
BfrI	CTTAAG	1 cut(s)	148
BfuAI	ACCTGC	1 cut(s)	729
BglII	AGATCT	5 cut(s)	1150, 1735, 2577, 2612, 2681
BisI	GCNGC	5 cut(s)	1359, 1516, 2302, 2407, 2509
BlnI	CCTAGG	1 cut(s)	2704
BlpI	GCTNAGC	1 cut(s)	2270
BlsI	GCNGC	5 cut(s)	1360, 1517, 2303, 2408, 2510
BmcAI	AGTACT	1 cut(s)	403
Bme1390I	CCNGG	8 cut(s)	253, 468, 609, 961, 1298, 1484, 1778, 2018
Bme18I	GGWCC	1 cut(s)	2748
BmgBI	CACGTC	1 cut(s)	1819
BmgT120I	GGNCC	1 cut(s)	2748
BmiI	GGNNCC	5 cut(s)	618, 753, 869, 1488, 2655
BmrFI	CCNGG	8 cut(s)	253, 468, 609, 961, 1298, 1484, 1778, 2018
BmrI	ACTGGG	1 cut(s)	934
BmsI	GCATC	7 cut(s)	14, 82, 552, 896, 965, 1110, 1384
BmtI	GCTAGC	1 cut(s)	1923
BmuI	ACTGGG	1 cut(s)	934
BoxI	GACNNNNGTC	1 cut(s)	67
BpiI	GAAGAC	4 cut(s)	953, 973, 1594, 2562
BpmI	CTGGAG	2 cut(s)	895, 2604
Bpu1102I	GCTNAGC	1 cut(s)	2270
BpuEI	CTTGAG	4 cut(s)	713, 2304, 2670, 2810
BpuMI	CCSGG	2 cut(s)	253, 961
BsaAI	YACGTR	1 cut(s)	2089
BsaBI	GATNNNNATC	1 cut(s)	1313
BsaI	GGTCTC	2 cut(s)	711, 1495
BsaJI	CCNNGG	8 cut(s)	466, 585, 651, 1096, 1482, 1490, 2704, 2806
BsaWI	WCCGGW	1 cut(s)	2750
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bsc4I	CCNNNNNNNGG	5 cut(s)	1101, 1102, 1630, 1631, 1777
Bse118I	RCCGGY	2 cut(s)	870, 2750
Bse1I	ACTGG	8 cut(s)	84, 506, 528, 929, 1383, 1402, 1569, 2657
Bse21I	CCTNAGG	1 cut(s)	786
Bse3DI	GCAATG	1 cut(s)	1257
Bse8I	GATNNNNATC	1 cut(s)	1313
BseBI	CCWGG	6 cut(s)	468, 609, 1298, 1484, 1778, 2018
BseDI	CCNNGG	8 cut(s)	466, 585, 651, 1096, 1482, 1490, 2704, 2806
BseGI	GGATG	9 cut(s)	532, 956, 1306, 1314, 1399, 2026, 2098, 2203, 2545
BseJI	GATNNNNATC	1 cut(s)	1313
BseLI	CCNNNNNNNGG	5 cut(s)	1101, 1102, 1630, 1631, 1777
BseMI	GCAATG	1 cut(s)	1257
BseMII	CTCAG	7 cut(s)	704, 1051, 1343, 1401, 1479, 2397, 2588
BseNI	ACTGG	8 cut(s)	84, 506, 528, 929, 1383, 1402, 1569, 2657
BseSI	GKGCMC	3 cut(s)	621, 1422, 2703
BseXI	GCAGC	5 cut(s)	1345, 1527, 2288, 2418, 2495
BsgI	GTGCAG	1 cut(s)	1728
BshFI	GGCC	2 cut(s)	369, 379
BshNI	GGYRCC	3 cut(s)	616, 867, 1486
BshTI	ACCGGT	1 cut(s)	2750
BsiHKAI	GWGCWC	2 cut(s)	2703, 2758
BsiSI	CCGG	4 cut(s)	253, 871, 960, 2751
BslFI	GGGAC	5 cut(s)	580, 982, 1118, 2468, 2799
BslI	CCNNNNNNNGG	5 cut(s)	1101, 1102, 1630, 1631, 1777
BsmAI	GTCTC	5 cut(s)	161, 711, 1495, 1712, 2207
BsmFI	GGGAC	5 cut(s)	580, 982, 1118, 2468, 2799
BsmI	GAATGC	2 cut(s)	1915, 2384
BsnI	GGCC	2 cut(s)	369, 379
Bso31I	GGTCTC	2 cut(s)	711, 1495
Bsp1286I	GDGCHC	4 cut(s)	621, 1422, 2703, 2758
Bsp1407I	TGTACA	1 cut(s)	2227
Bsp1720I	GCTNAGC	1 cut(s)	2270
Bsp19I	CCATGG	1 cut(s)	585
BspACI	CCGC	1 cut(s)	2139
BspANI	GGCC	2 cut(s)	369, 379
BspCNI	CTCAG	7 cut(s)	705, 1050, 1342, 1400, 1478, 2396, 2587
BspLI	GGNNCC	5 cut(s)	618, 753, 869, 1488, 2655
BspMI	ACCTGC	1 cut(s)	729
BspOI	GCTAGC	1 cut(s)	1923
BspPI	GGATC	6 cut(s)	599, 746, 759, 776, 912, 1540
BspQI	GCTCTTC	2 cut(s)	126, 2763
BspT107I	GGYRCC	3 cut(s)	616, 867, 1486
BspTI	CTTAAG	1 cut(s)	148
BspTNI	GGTCTC	2 cut(s)	711, 1495
BsrDI	GCAATG	1 cut(s)	1257
BsrFI	RCCGGY	2 cut(s)	870, 2750
BsrGI	TGTACA	1 cut(s)	2227
BsrI	ACTGG	8 cut(s)	84, 506, 528, 929, 1383, 1402, 1569, 2657
BssAI	RCCGGY	2 cut(s)	870, 2750
BssECI	CCNNGG	8 cut(s)	466, 585, 651, 1096, 1482, 1490, 2704, 2806
BssT1I	CCWWGG	5 cut(s)	585, 1096, 1490, 2704, 2806
Bst2UI	CCWGG	6 cut(s)	468, 609, 1298, 1484, 1778, 2018
Bst6I	CTCTTC	3 cut(s)	126, 1551, 2763
BstAFI	CTTAAG	1 cut(s)	148
BstAUI	TGTACA	1 cut(s)	2227
BstBAI	YACGTR	1 cut(s)	2089
BstC8I	GCNNGC	8 cut(s)	541, 648, 1070, 1864, 1917, 1921, 2046, 2358
BstDSI	CCRYGG	2 cut(s)	585, 651
BstF5I	GGATG	9 cut(s)	532, 956, 1306, 1314, 1399, 2026, 2098, 2203, 2545
BstH2I	RGCGCY	1 cut(s)	1297
BstHHI	GCGC	2 cut(s)	1296, 1915
BstMAI	GTCTC	5 cut(s)	161, 711, 1495, 1712, 2207
BstMWI	GCNNNNNNNGC	2 cut(s)	632, 1190
BstNI	CCWGG	6 cut(s)	468, 609, 1298, 1484, 1778, 2018
BstNSI	RCATGY	3 cut(s)	1461, 2269, 2598
BstPAI	GACNNNNGTC	1 cut(s)	67
BstSCI	CCNGG	8 cut(s)	251, 466, 607, 959, 1296, 1482, 1776, 2016
BstSLI	GKGCMC	3 cut(s)	621, 1422, 2703
BstV1I	GCAGC	5 cut(s)	1345, 1527, 2288, 2418, 2495
BstV2I	GAAGAC	4 cut(s)	953, 973, 1594, 2562
BstX2I	RGATCY	9 cut(s)	604, 751, 781, 917, 1150, 1735, 2577, 2612, 2681
BstXI	CCANNNNNNTGG	1 cut(s)	352
BstYI	RGATCY	9 cut(s)	604, 751, 781, 917, 1150, 1735, 2577, 2612, 2681
Bsu36I	CCTNAGG	1 cut(s)	786
BsuRI	GGCC	2 cut(s)	369, 379
BtgI	CCRYGG	2 cut(s)	585, 651
BtgZI	GCGATG	1 cut(s)	459
BtrI	CACGTC	1 cut(s)	1819
BtsCI	GGATG	9 cut(s)	532, 956, 1306, 1314, 1399, 2026, 2098, 2203, 2545
BtsIMutI	CAGTG	6 cut(s)	177, 521, 635, 922, 1224, 1395
BveI	ACCTGC	1 cut(s)	729
Cac8I	GCNNGC	8 cut(s)	541, 648, 1070, 1864, 1917, 1921, 2046, 2358
CfoI	GCGC	2 cut(s)	1296, 1915
Cfr10I	RCCGGY	2 cut(s)	870, 2750
Cfr13I	GGNCC	1 cut(s)	2748
CseI	GACGC	1 cut(s)	2465
CsiI	ACCWGGT	1 cut(s)	1776
Csp6I	GTAC	7 cut(s)	213, 402, 1052, 1353, 1487, 2228, 2592
CspAI	ACCGGT	1 cut(s)	2750
CviQI	GTAC	7 cut(s)	213, 402, 1052, 1353, 1487, 2228, 2592
EaeI	YGGCCR	1 cut(s)	367
Eam1104I	CTCTTC	3 cut(s)	126, 1551, 2763
EarI	CTCTTC	3 cut(s)	126, 1551, 2763
Eco130I	CCWWGG	5 cut(s)	585, 1096, 1490, 2704, 2806
Eco147I	AGGCCT	1 cut(s)	379
Eco31I	GGTCTC	2 cut(s)	711, 1495
Eco32I	GATATC	1 cut(s)	1814
Eco47I	GGWCC	1 cut(s)	2748
Eco57I	CTGAAG	4 cut(s)	150, 165, 965, 1575
Eco72I	CACGTG	1 cut(s)	2089
Eco81I	CCTNAGG	1 cut(s)	786
EcoRI	GAATTC	3 cut(s)	594, 1568, 2798
EcoRII	CCWGG	6 cut(s)	466, 607, 1296, 1482, 1776, 2016
EcoRV	GATATC	1 cut(s)	1814
EcoT14I	CCWWGG	5 cut(s)	585, 1096, 1490, 2704, 2806
ErhI	CCWWGG	5 cut(s)	585, 1096, 1490, 2704, 2806
FalI	AAGNNNNNCTT	8 cut(s)	1080, 1112, 1647, 1679, 1846, 1878, 2543, 2575
FaqI	GGGAC	5 cut(s)	580, 982, 1118, 2468, 2799
FauI	CCCGC	1 cut(s)	2146
FauNDI	CATATG	1 cut(s)	553
Fnu4HI	GCNGC	5 cut(s)	1359, 1516, 2302, 2407, 2509
FokI	GGATG	9 cut(s)	539, 943, 1313, 1321, 1406, 2033, 2105, 2210, 2552
Fsp4HI	GCNGC	5 cut(s)	1359, 1516, 2302, 2407, 2509
FspBI	CTAG	8 cut(s)	59, 971, 1920, 1955, 2240, 2648, 2705, 2762
GlaI	GCGC	2 cut(s)	1295, 1914
GluI	GCNGC	5 cut(s)	1359, 1516, 2302, 2407, 2509
GsuI	CTGGAG	2 cut(s)	895, 2604
HaeII	RGCGCY	1 cut(s)	1297
HaeIII	GGCC	2 cut(s)	369, 379
HapII	CCGG	4 cut(s)	253, 871, 960, 2751
HgaI	GACGC	1 cut(s)	2465
HhaI	GCGC	2 cut(s)	1296, 1915
Hin6I	GCGC	2 cut(s)	1294, 1913
HinP1I	GCGC	2 cut(s)	1294, 1913
HincII	GTYRAC	4 cut(s)	882, 1326, 2065, 2338
HindII	GTYRAC	4 cut(s)	882, 1326, 2065, 2338
HindIII	AAGCTT	1 cut(s)	2042
HpaII	CCGG	4 cut(s)	253, 871, 960, 2751
HphI	GGTGA	5 cut(s)	574, 803, 1779, 1841, 2381
Hpy166II	GTNNAC	6 cut(s)	882, 1326, 1699, 2065, 2338, 2701
Hpy8I	GTNNAC	6 cut(s)	882, 1326, 1699, 2065, 2338, 2701
Hpy99I	CGWCG	1 cut(s)	1823
HpyCH4IV	ACGT	3 cut(s)	1818, 2088, 2151
HpyF10VI	GCNNNNNNNGC	2 cut(s)	632, 1190
HpySE526I	ACGT	3 cut(s)	1818, 2088, 2151
HspAI	GCGC	2 cut(s)	1294, 1913
KpnI	GGTACC	1 cut(s)	1490
LguI	GCTCTTC	2 cut(s)	126, 2763
Lsp1109I	GCAGC	5 cut(s)	1345, 1527, 2288, 2418, 2495
LweI	GCATC	7 cut(s)	14, 82, 552, 896, 965, 1110, 1384
MabI	ACCWGGT	1 cut(s)	1776
MaeI	CTAG	8 cut(s)	59, 971, 1920, 1955, 2240, 2648, 2705, 2762
MaeII	ACGT	3 cut(s)	1818, 2088, 2151
MaeIII	GTNAC	4 cut(s)	440, 899, 1780, 2177
MfeI	CAATTG	2 cut(s)	627, 2410
MflI	RGATCY	9 cut(s)	604, 751, 781, 917, 1150, 1735, 2577, 2612, 2681
MhlI	GDGCHC	4 cut(s)	621, 1422, 2703, 2758
MlyI	GAGTC	5 cut(s)	1085, 1100, 1214, 1936, 2343
MroXI	GAANNNNTTC	4 cut(s)	45, 578, 594, 1648
MslI	CAYNNNNRTG	3 cut(s)	350, 1792, 2091
MspA1I	CMGCKG	1 cut(s)	1358
MspCI	CTTAAG	1 cut(s)	148
MspI	CCGG	4 cut(s)	253, 871, 960, 2751
MspR9I	CCNGG	8 cut(s)	253, 468, 609, 961, 1298, 1484, 1778, 2018
MunI	CAATTG	2 cut(s)	627, 2410
Mva1269I	GAATGC	2 cut(s)	1915, 2384
MvaI	CCWGG	6 cut(s)	468, 609, 1298, 1484, 1778, 2018
MwoI	GCNNNNNNNGC	2 cut(s)	632, 1190
NciI	CCSGG	2 cut(s)	253, 961
NcoI	CCATGG	1 cut(s)	585
NdeI	CATATG	1 cut(s)	553
NheI	GCTAGC	1 cut(s)	1919
NlaIV	GGNNCC	5 cut(s)	618, 753, 869, 1488, 2655
NmuCI	GTSAC	1 cut(s)	2177
NspI	RCATGY	3 cut(s)	1461, 2269, 2598
PceI	AGGCCT	1 cut(s)	379
PciSI	GCTCTTC	2 cut(s)	126, 2763
PctI	GAATGC	2 cut(s)	1915, 2384
PdmI	GAANNNNTTC	4 cut(s)	45, 578, 594, 1648
PfeI	GAWTC	7 cut(s)	187, 395, 1258, 1314, 1801, 2186, 2468
PfoI	TCCNGGA	3 cut(s)	607, 959, 2016
PinAI	ACCGGT	1 cut(s)	2750
PkrI	GCNGC	5 cut(s)	1360, 1517, 2303, 2408, 2510
PleI	GAGTC	5 cut(s)	1085, 1100, 1214, 1935, 2342
PmaCI	CACGTG	1 cut(s)	2089
PmlI	CACGTG	1 cut(s)	2089
PpsI	GAGTC	5 cut(s)	1085, 1100, 1214, 1935, 2342
Ppu21I	YACGTR	1 cut(s)	2089
PshAI	GACNNNNGTC	1 cut(s)	67
PshBI	ATTAAT	1 cut(s)	2630
Psp6I	CCWGG	6 cut(s)	466, 607, 1296, 1482, 1776, 2016
PspCI	CACGTG	1 cut(s)	2089
PspGI	CCWGG	6 cut(s)	466, 607, 1296, 1482, 1776, 2016
PspN4I	GGNNCC	5 cut(s)	618, 753, 869, 1488, 2655
PspPI	GGNCC	1 cut(s)	2748
PsuI	RGATCY	9 cut(s)	604, 751, 781, 917, 1150, 1735, 2577, 2612, 2681
PvuII	CAGCTG	1 cut(s)	1358
RsaI	GTAC	7 cut(s)	214, 403, 1053, 1354, 1488, 2229, 2593
RsaNI	GTAC	7 cut(s)	213, 402, 1052, 1353, 1487, 2228, 2592
RseI	CAYNNNNRTG	3 cut(s)	350, 1792, 2091
SapI	GCTCTTC	2 cut(s)	126, 2763
SatI	GCNGC	5 cut(s)	1359, 1516, 2302, 2407, 2509
Sau96I	GGNCC	1 cut(s)	2748
ScaI	AGTACT	1 cut(s)	403
SchI	GAGTC	5 cut(s)	1085, 1100, 1214, 1936, 2343
ScrFI	CCNGG	8 cut(s)	253, 468, 609, 961, 1298, 1484, 1778, 2018
SduI	GDGCHC	4 cut(s)	621, 1422, 2703, 2758
SexAI	ACCWGGT	1 cut(s)	1776
SfaNI	GCATC	7 cut(s)	14, 82, 552, 896, 965, 1110, 1384
SinI	GGWCC	1 cut(s)	2748
SmiMI	CAYNNNNRTG	3 cut(s)	350, 1792, 2091
SmlI	CTYRAG	5 cut(s)	148, 692, 2319, 2685, 2789
SmoI	CTYRAG	5 cut(s)	148, 692, 2319, 2685, 2789
SseBI	AGGCCT	1 cut(s)	379
SsiI	CCGC	1 cut(s)	2139
SspI	AATATT	2 cut(s)	319, 2164
SspMI	CTAG	8 cut(s)	59, 971, 1920, 1955, 2240, 2648, 2705, 2762
StuI	AGGCCT	1 cut(s)	379
StyD4I	CCNGG	8 cut(s)	251, 466, 607, 959, 1296, 1482, 1776, 2016
StyI	CCWWGG	5 cut(s)	585, 1096, 1490, 2704, 2806
TaiI	ACGT	3 cut(s)	1821, 2091, 2154
TaqI	TCGA	4 cut(s)	322, 823, 890, 1887
TatI	WGTACW	3 cut(s)	401, 1352, 2227
TfiI	GAWTC	7 cut(s)	187, 395, 1258, 1314, 1801, 2186, 2468
TscAI	CASTG	6 cut(s)	177, 528, 642, 929, 1231, 1395
TseFI	GTSAC	1 cut(s)	2177
TseI	GCWGC	5 cut(s)	1358, 1515, 2301, 2406, 2508
Tsp45I	GTSAC	1 cut(s)	2177
TspGWI	ACGGA	2 cut(s)	668, 1603
TspRI	CASTG	6 cut(s)	177, 528, 642, 929, 1231, 1395
Vha464I	CTTAAG	1 cut(s)	148
VneI	GTGCAC	1 cut(s)	2699
VpaK11BI	GGWCC	1 cut(s)	2748
VspI	ATTAAT	1 cut(s)	2630
XapI	RAATTY	9 cut(s)	296, 594, 1341, 1568, 1618, 2012, 2421, 2733, 2798
XbaI	TCTAGA	1 cut(s)	2647
XceI	RCATGY	3 cut(s)	1461, 2269, 2598
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1375, 2292
XmaJI	CCTAGG	1 cut(s)	2704
XmnI	GAANNNNTTC	4 cut(s)	45, 578, 594, 1648
XspI	CTAG	8 cut(s)	59, 971, 1920, 1955, 2240, 2648, 2705, 2762
ZrmI	AGTACT	1 cut(s)	403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.