Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004739

Physical Location & Seq

Genomic Coordinates Reverse (-)

82516 .. 89259

Gene Structure 6744 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1001300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGAGGAAAGTCATGGCATCAAGAGACCCTTCCCACCACTCTCTTTCTGAAACAATTCATTCCTTACTAGGACTGAAGTCACATCTCACTTCCACCTGGGTTCAATCTGTTTGTGATATAATAACAAACCTACCTCCTCACTGTCCTCAACCTCAAGGACAACCCACCGACTTAACAACAACAAATCTCGAGACAGACACTTCTTCAGACGCTGCCTCAAAAATTAGAGATGAACTTGCTATCTTGACTGCCCACATGAATGAATTTAACAAACGGAGAAGGCAGGTTCTAAATGAATTTCTAAATTTGAAAGGTAATATTAGAGTGCTTTGTCGTATAAGACCCATTGCAATAGGGGAGAACTTCGGCCGCTTTAGGCCTGTTGTAGCATTGGATTCAAGTAATGTTCTTCTAAGACTTACTGACAGTAAGAATAAAAGTTACAGTTTCGACAATGTTTTCCGCCCCGGCTCATCACAAGACGAAGTCTTTTCAGAAGTTGAGCCAGTCATCAAATCCGCACTTGACGGCTACAATGCATGCATATTCGCTTATGGACAGACAGGCACAGGGAAAACCTTCACCATGGAAGGAACTCCAGAATTTCCCGGAGTGGTGCCCCGTGCAATTGAAGCACTGTTTAGGCAAGCAGTTGATAGCAACCATGTGTTTCTATTCAGTTTCAGTATGCTTGAGATTTACATGGGAAATCTCAGAGACCTGCTAATTTCTCATCCAACAAAGGCAATGGATCCTCTGCCTCCCTGCCTATCAATCCAATCAGATCCAAATGGTGGGATTGAAATTGAAAACCTTGTGGCTATTCAAGTGAGTGACTTCAACCAAGCTTTGAAACTATATAAACTAGGGTGCCGGTTCAGGTCAACTGCATCAACAAATTGTAACAGTACTTCCAGCAGATCACACTGCTTGATTCGCATATCAATCACATGTAGTGGAGCACCTGAGAGGCGAAGGGAAACAAATAAAGTTTGGTTTGTTGATCTTGGAGGAAGCGAGCGTTTGTTGAAAACAAAGGCATGGGGTAGAAGACTAGAAGAAGGAAAGGCCATTAACTTGTCACTTTCCTCTCTTGGAGATGTCATCAGTGCCCTTCAAAATAGAAAAAGCCATGTACCTTATAGGAATAGCAAGCTGACGCAGGTTCTTAAGGATTCCCTTGGACAGGACTCAAAAACTCTAATGCTTGTCCATATCAGTCCTAATGAAGAAGATCTCTGTGAAACAGTGTGTTCTTTGAATTTTGCGACTAGGGTGAGAAGCGTTCATCTAGGGAAAACAGATTCGACTGTAGAAAGGGAGAAGGAAGTTGCAATGATCGATCTGCAACAAAAGATTAAACTGATTGAAGCTGAGCGCCAAGAAGCTAGAACGAGTATCAAGAAGTTGACTGAGGAATTGGATAAATTAACAGGGACATCCCCATCTTTCGGTGAGCAACTGGATGCTTACCATTTATATAAATATAACGAACGGCCCCAACCTAATCTTGAAATTAGTAGGAACAAGACTAGAAATATTGCAGCAGCTCCATTATCGCAGGTACCAAGGTTTATGAGGCCTACTGTTTGCAGTCGAAGAAAATCTGGACTGGAACACCTAACTTCTGAAGAAACAGTGAGAGTTCCTGCAAAAAGGAGAAGGCCAATGGACCATCATGCGAATTCTGTGAATTTCCCCTTAAAGGGTACTTCAGAAAGCAACTCAGAATGCAGTATTTCCAGAAATAGCTGTTTAGTGGCTTTGAAGGTGAAAAGTAGTGCAGATTGTGAAACAGAATACAGTCAGGATCTATCAGAGTCCGATACTAAAGAGGTTGTGCTACCAGGACAGGAAGCATCACCTAAACCCTATGTTGATAAGTGGTTACTCTTAAATAACAATGCAAATGTTACCAGGAGTCACTTTCACAGGAACAAATGGATTCCAGCCATTCCTACCCCAGAGAAGAAACATATATGTAATGGACAGAAAGAAACAGACAATTTGCAAGATAAGAAAGTCCATAATTACAAGTTTGCAACGAAACAGATTATCAACCACAACAAATTGGAGAAGCATGCTTGTGTGGAGGGATCTCAGAGGTCCGTGCAAGAAGTGGTTATTAATAAAACTCCCACAAATTTGAAGGATTATGACAGTATTTCCTCAAGATGTAACTATATAAAAGATAAACTTGATGGCATGCCAATGGAGAAACACATATGCAACACTATATATCCATCTGATATTTGGTGCAGCAGTTCTCATTTGAATCAAGATGATAATGGGGTCCACGCTTCATCCACAACGCAGGCACTACTTGGTGAAACAGAATGCTCAGACAGTTCCTTGTCAGGTGATAGTAATTGGTGCCAAATTTCTACCACCAATTCAGCTTATAGCGTTTTGGACTCAAGAAAAGAGTCTAGTATCTCAGTTTTGAGAATAGAACTAAAGTCATGCTGTCAACAATTGTGTACCGCAATATGTGAGGAAGATTGTGGAAAAGAAGACTCGGACACGCTATTTAAGAGGTCTGCTGAAGGGATAAGACCGGGCCTAGGAAAAATGAGATGTCAAAGAGCTTTATTTATGGAAAATGCAACTCCAAAGGAACCAACCAAGCCACAACAACTCATAAAGTCACAAGAAGATGATATGAACTCAGGAATATGCCACCATCTTAAACAGAAAATTCAAATTTTGTGGATCAGTACTCTTCTAGGGTTAGGATTACGAAATTTGGGACTAGAGCAGGAATTCTTCCTTGGCTTAATGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

929

Amino Acids

104.06

Weight (kDa)

8.36

Isoelectric Point (pI)

52.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	88 - 242	7.8e-35	Microtubule binding
Kinesin	PF00225	139 - 429	6.5e-96	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55550
fragaria_vesca	FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040 FvH4_3g46040
malus_domestica	MD03G1001300.v1.1
prunus_persica	Prupe.6G000800_v2.0.a1
pyrus_communis	pycom03g00110
rosa_chinensis	RchiOBHm_Chr5g0084121
rosa_laevigata	RLG00000037083
rosa_multiflora	Rmu_ssc0000372.1_g000094
rosa_roxburghii	Rroxscaffold_1G00000520
rosa_rugosa	Rorug05G0489200 Rorug05G0489200 Rorug05G0489200 Rorug05G0489300
rosa_samantha	Rh5AG541800 Rh5BG567400 Rh5CG589200 Rh5DG575600
rosa_wichuraiana	Rw5G050400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	4 cut(s)	274, 729, 1153, 1552
Acc65I	GGTACC	1 cut(s)	1564
AccB1I	GGYRCC	4 cut(s)	616, 870, 1564, 2373
AccB7I	CCANNNNNTGG	1 cut(s)	794
AciI	CCGC	4 cut(s)	370, 463, 519, 2484
AclWI	GGATC	6 cut(s)	746, 759, 779, 1818, 2104, 2720
AcoI	YGGCCR	1 cut(s)	367
AcuI	CTGAAG	5 cut(s)	95, 189, 1650, 1698, 2565
AfaI	GTAC	6 cut(s)	910, 1137, 1566, 1711, 2482, 2719
AfiI	CCNNNNNNNGG	6 cut(s)	794, 1186, 1451, 1705, 1706, 2564
AflII	CTTAAG	1 cut(s)	1169
AflIII	ACRYGT	1 cut(s)	950
AjnI	CCWGG	3 cut(s)	95, 1846, 1916
AjuI	GAANNNNNNNTTGG	2 cut(s)	2754, 2786
AluBI	AGCT	8 cut(s)	848, 1156, 1373, 1388, 1550, 1752, 2399, 2588
AluI	AGCT	8 cut(s)	848, 1156, 1373, 1388, 1550, 1752, 2399, 2588
Alw21I	GWGCWC	1 cut(s)	964
Alw26I	GTCTC	3 cut(s)	18, 185, 711
AlwI	GGATC	6 cut(s)	746, 759, 779, 1818, 2104, 2720
AlwNI	CAGNNNCTG	1 cut(s)	212
Ama87I	CYCGRG	1 cut(s)	188
AoxI	GGCC	7 cut(s)	367, 377, 1068, 1496, 1580, 1664, 2560
ApeKI	GCWGC	4 cut(s)	212, 1544, 1547, 2259
AseI	ATTAAT	1 cut(s)	2127
Asp700I	GAANNNNTTC	2 cut(s)	54, 578
Asp718I	GGTACC	1 cut(s)	1564
AspA2I	CCTAGG	1 cut(s)	2563
AspLEI	GCGC	1 cut(s)	1380
AspS9I	GGNCC	5 cut(s)	1497, 1672, 2106, 2293, 2560
AsuC2I	CCSGG	3 cut(s)	468, 609, 2559
AsuHPI	GGTGA	7 cut(s)	574, 1288, 1466, 1783, 1854, 2339, 2372
AvaI	CYCGRG	1 cut(s)	188
AvaII	GGWCC	3 cut(s)	1672, 2106, 2293
AvrII	CCTAGG	1 cut(s)	2563
BaeGI	GKGCMC	2 cut(s)	621, 1114
BaeI	ACNNNNGTAYC	2 cut(s)	1556, 1589
BamHI	GGATCC	1 cut(s)	751
BanI	GGYRCC	4 cut(s)	616, 870, 1564, 2373
BarI	GAAGNNNNNNTAC	2 cut(s)	895, 927
BbsI	GAAGAC	2 cut(s)	1057, 2520
Bbv12I	GWGCWC	1 cut(s)	964
BbvI	GCAGC	4 cut(s)	199, 1556, 1559, 2271
BccI	CCATC	5 cut(s)	1453, 1683, 2195, 2251, 2691
BceAI	ACGGC	2 cut(s)	544, 1511
BciT130I	CCWGG	3 cut(s)	97, 1848, 1918
BcnI	CCSGG	3 cut(s)	468, 609, 2559
BcoDI	GTCTC	3 cut(s)	18, 185, 711
BfmI	CTRYAG	1 cut(s)	1311
BfoI	RGCGCY	1 cut(s)	1381
BfrI	CTTAAG	1 cut(s)	1169
BfuAI	ACCTGC	4 cut(s)	274, 729, 1153, 1552
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	5 cut(s)	213, 370, 1545, 1548, 2260
BlnI	CCTAGG	1 cut(s)	2563
BlpI	GCTNAGC	1 cut(s)	1374
BlsI	GCNGC	5 cut(s)	214, 371, 1546, 1549, 2261
BmcAI	AGTACT	2 cut(s)	910, 2719
Bme1390I	CCNGG	6 cut(s)	97, 468, 609, 1848, 1918, 2559
Bme18I	GGWCC	3 cut(s)	1672, 2106, 2293
BmeT110I	CYCGRG	1 cut(s)	188
BmgT120I	GGNCC	5 cut(s)	1497, 1672, 2106, 2293, 2560
BmiI	GGNNCC	8 cut(s)	618, 753, 872, 1499, 1566, 2294, 2375, 2619
BmrFI	CCNGG	6 cut(s)	97, 468, 609, 1848, 1918, 2559
BmsI	GCATC	4 cut(s)	26, 899, 1456, 1868
BoxI	GACNNNNGTC	1 cut(s)	76
BpiI	GAAGAC	2 cut(s)	1057, 2520
BpmI	CTGGAG	1 cut(s)	582
Bpu1102I	GCTNAGC	1 cut(s)	1374
BpuEI	CTTGAG	4 cut(s)	138, 713, 2155, 2401
BpuMI	CCSGG	3 cut(s)	468, 609, 2559
Bsa29I	ATCGAT	1 cut(s)	1341
BsaI	GGTCTC	2 cut(s)	18, 711
BsaJI	CCNNGG	7 cut(s)	96, 466, 585, 1180, 1568, 2563, 2770
Bsc4I	CCNNNNNNNGG	6 cut(s)	794, 1186, 1451, 1705, 1706, 2564
Bse118I	RCCGGY	1 cut(s)	873
Bse1I	ACTGG	3 cut(s)	506, 1467, 1617
Bse3DI	GCAATG	3 cut(s)	345, 753, 1341
BseBI	CCWGG	3 cut(s)	97, 1848, 1918
BseCI	ATCGAT	1 cut(s)	1341
BseDI	CCNNGG	7 cut(s)	96, 466, 585, 1180, 1568, 2563, 2770
BseGI	GGATG	4 cut(s)	733, 1439, 1471, 2303
BseLI	CCNNNNNNNGG	6 cut(s)	794, 1186, 1451, 1705, 1706, 2564
BseMI	GCAATG	3 cut(s)	345, 753, 1341
BseMII	CTCAG	9 cut(s)	727, 957, 1365, 1404, 1740, 2114, 2355, 2451, 2682
BseNI	ACTGG	3 cut(s)	506, 1467, 1617
BseRI	GAGGAG	1 cut(s)	126
BseSI	GKGCMC	2 cut(s)	621, 1114
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	4 cut(s)	199, 1556, 1559, 2271
BsgI	GTGCAG	2 cut(s)	1803, 2278
Bsh1285I	CGRYCG	1 cut(s)	370
BshFI	GGCC	7 cut(s)	369, 379, 1070, 1498, 1582, 1666, 2562
BshNI	GGYRCC	4 cut(s)	616, 870, 1564, 2373
BshVI	ATCGAT	1 cut(s)	1341
BsiEI	CGRYCG	1 cut(s)	370
BsiHKAI	GWGCWC	1 cut(s)	964
BsiHKCI	CYCGRG	1 cut(s)	188
BsiSI	CCGG	4 cut(s)	468, 609, 874, 2558
BslFI	GGGAC	2 cut(s)	1450, 2763
BslI	CCNNNNNNNGG	6 cut(s)	794, 1186, 1451, 1705, 1706, 2564
BsmAI	GTCTC	3 cut(s)	18, 185, 711
BsmFI	GGGAC	2 cut(s)	1450, 2763
BsmI	GAATGC	2 cut(s)	1736, 2342
BsnI	GGCC	7 cut(s)	369, 379, 1070, 1498, 1582, 1666, 2562
Bso31I	GGTCTC	2 cut(s)	18, 711
BsoBI	CYCGRG	1 cut(s)	188
Bsp1286I	GDGCHC	3 cut(s)	621, 964, 1114
Bsp1720I	GCTNAGC	1 cut(s)	1374
Bsp19I	CCATGG	1 cut(s)	585
BspACI	CCGC	4 cut(s)	370, 463, 519, 2484
BspANI	GGCC	7 cut(s)	369, 379, 1070, 1498, 1582, 1666, 2562
BspCNI	CTCAG	9 cut(s)	726, 958, 1366, 1405, 1739, 2113, 2354, 2450, 2681
BspDI	ATCGAT	1 cut(s)	1341
BspLI	GGNNCC	8 cut(s)	618, 753, 872, 1499, 1566, 2294, 2375, 2619
BspMI	ACCTGC	4 cut(s)	274, 729, 1153, 1552
BspPI	GGATC	6 cut(s)	746, 759, 779, 1818, 2104, 2720
BspT107I	GGYRCC	4 cut(s)	616, 870, 1564, 2373
BspTI	CTTAAG	1 cut(s)	1169
BspTNI	GGTCTC	2 cut(s)	18, 711
BsrDI	GCAATG	3 cut(s)	345, 753, 1341
BsrFI	RCCGGY	1 cut(s)	873
BsrI	ACTGG	3 cut(s)	506, 1467, 1617
BssAI	RCCGGY	1 cut(s)	873
BssECI	CCNNGG	7 cut(s)	96, 466, 585, 1180, 1568, 2563, 2770
BssT1I	CCWWGG	5 cut(s)	585, 1180, 1568, 2563, 2770
Bst2UI	CCWGG	3 cut(s)	97, 1848, 1918
Bst6I	CTCTTC	1 cut(s)	2727
BstAFI	CTTAAG	1 cut(s)	1169
BstC8I	GCNNGC	7 cut(s)	541, 648, 1019, 1154, 2082, 2207, 2316
BstDSI	CCRYGG	1 cut(s)	585
BstENI	CCTNNNNNAGG	1 cut(s)	1184
BstF5I	GGATG	4 cut(s)	733, 1439, 1471, 2303
BstH2I	RGCGCY	1 cut(s)	1381
BstHHI	GCGC	1 cut(s)	1380
BstMAI	GTCTC	3 cut(s)	18, 185, 711
BstMCI	CGRYCG	1 cut(s)	370
BstMWI	GCNNNNNNNGC	2 cut(s)	632, 936
BstNI	CCWGG	3 cut(s)	97, 1848, 1918
BstNSI	RCATGY	4 cut(s)	543, 954, 2084, 2209
BstPAI	GACNNNNGTC	1 cut(s)	76
BstSCI	CCNGG	6 cut(s)	95, 466, 607, 1846, 1916, 2557
BstSFI	CTRYAG	1 cut(s)	1311
BstSLI	GKGCMC	2 cut(s)	621, 1114
BstV1I	GCAGC	4 cut(s)	199, 1556, 1559, 2271
BstV2I	GAAGAC	2 cut(s)	1057, 2520
BstX2I	RGATCY	5 cut(s)	751, 784, 1234, 1810, 2096
BstYI	RGATCY	5 cut(s)	751, 784, 1234, 1810, 2096
BstZI	CGGCCG	1 cut(s)	367
Bsu15I	ATCGAT	1 cut(s)	1341
BsuRI	GGCC	7 cut(s)	369, 379, 1070, 1498, 1582, 1666, 2562
BsuTUI	ATCGAT	1 cut(s)	1341
BtgI	CCRYGG	1 cut(s)	585
BtsCI	GGATG	4 cut(s)	733, 1439, 1471, 2303
BtsI	GCAGTG	1 cut(s)	925
BtsIMutI	CAGTG	6 cut(s)	139, 635, 925, 1114, 1254, 1644
BveI	ACCTGC	4 cut(s)	274, 729, 1153, 1552
Cac8I	GCNNGC	7 cut(s)	541, 648, 1019, 1154, 2082, 2207, 2316
CaiI	CAGNNNCTG	1 cut(s)	212
CfoI	GCGC	1 cut(s)	1380
Cfr10I	RCCGGY	1 cut(s)	873
Cfr13I	GGNCC	5 cut(s)	1497, 1672, 2106, 2293, 2560
ClaI	ATCGAT	1 cut(s)	1341
CseI	GACGC	2 cut(s)	218, 1168
Csp6I	GTAC	6 cut(s)	909, 1136, 1565, 1710, 2481, 2718
CspCI	CAANNNNNGTGG	2 cut(s)	2690, 2725
CviQI	GTAC	6 cut(s)	909, 1136, 1565, 1710, 2481, 2718
EaeI	YGGCCR	1 cut(s)	367
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	2727
EarI	CTCTTC	1 cut(s)	2727
EciI	GGCGGA	1 cut(s)	452
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	5 cut(s)	585, 1180, 1568, 2563, 2770
Eco147I	AGGCCT	2 cut(s)	379, 1582
Eco31I	GGTCTC	2 cut(s)	18, 711
Eco47I	GGWCC	3 cut(s)	1672, 2106, 2293
Eco52I	CGGCCG	1 cut(s)	367
Eco57I	CTGAAG	5 cut(s)	95, 189, 1650, 1698, 2565
Eco88I	CYCGRG	1 cut(s)	188
EcoNI	CCTNNNNNAGG	1 cut(s)	1184
EcoRI	GAATTC	2 cut(s)	1684, 2762
EcoRII	CCWGG	3 cut(s)	95, 1846, 1916
EcoT14I	CCWWGG	5 cut(s)	585, 1180, 1568, 2563, 2770
EcoT22I	ATGCAT	2 cut(s)	541, 545
ErhI	CCWWGG	5 cut(s)	585, 1180, 1568, 2563, 2770
FalI	AAGNNNNNCTT	4 cut(s)	13, 45, 2181, 2213
FaqI	GGGAC	2 cut(s)	1450, 2763
FauNDI	CATATG	1 cut(s)	2225
Fnu4HI	GCNGC	5 cut(s)	213, 370, 1545, 1548, 2260
FokI	GGATG	4 cut(s)	720, 1426, 1478, 2290
Fsp4HI	GCNGC	5 cut(s)	213, 370, 1545, 1548, 2260
GlaI	GCGC	1 cut(s)	1379
GluI	GCNGC	5 cut(s)	213, 370, 1545, 1548, 2260
GsuI	CTGGAG	1 cut(s)	582
HaeII	RGCGCY	1 cut(s)	1381
HaeIII	GGCC	7 cut(s)	369, 379, 1070, 1498, 1582, 1666, 2562
HapII	CCGG	4 cut(s)	468, 609, 874, 2558
HgaI	GACGC	2 cut(s)	218, 1168
HhaI	GCGC	1 cut(s)	1380
Hin6I	GCGC	1 cut(s)	1378
HinP1I	GCGC	1 cut(s)	1378
HincII	GTYRAC	3 cut(s)	885, 1410, 2471
HindII	GTYRAC	3 cut(s)	885, 1410, 2471
HindIII	AAGCTT	1 cut(s)	846
HpaII	CCGG	4 cut(s)	468, 609, 874, 2558
HphI	GGTGA	7 cut(s)	574, 1288, 1466, 1783, 1854, 2339, 2372
Hpy166II	GTNNAC	5 cut(s)	885, 1410, 2296, 2471, 2481
Hpy8I	GTNNAC	5 cut(s)	885, 1410, 2296, 2471, 2481
HpyF10VI	GCNNNNNNNGC	2 cut(s)	632, 936
HspAI	GCGC	1 cut(s)	1378
KpnI	GGTACC	1 cut(s)	1568
LmnI	GCTCC	2 cut(s)	959, 1555
Lsp1109I	GCAGC	4 cut(s)	199, 1556, 1559, 2271
LweI	GCATC	4 cut(s)	26, 899, 1456, 1868
MfeI	CAATTG	2 cut(s)	627, 2474
MflI	RGATCY	5 cut(s)	751, 784, 1234, 1810, 2096
MhlI	GDGCHC	3 cut(s)	621, 964, 1114
MlyI	GAGTC	6 cut(s)	1184, 1829, 1930, 2408, 2435, 2510
MmeI	TCCRAC	1 cut(s)	761
Mph1103I	ATGCAT	2 cut(s)	541, 545
MroXI	GAANNNNTTC	2 cut(s)	54, 578
MslI	CAYNNNNRTG	3 cut(s)	258, 2085, 2210
MspCI	CTTAAG	1 cut(s)	1169
MspI	CCGG	4 cut(s)	468, 609, 874, 2558
MspR9I	CCNGG	6 cut(s)	97, 468, 609, 1848, 1918, 2559
MunI	CAATTG	2 cut(s)	627, 2474
Mva1269I	GAATGC	2 cut(s)	1736, 2342
MvaI	CCWGG	3 cut(s)	97, 1848, 1918
MwoI	GCNNNNNNNGC	2 cut(s)	632, 936
NciI	CCSGG	3 cut(s)	468, 609, 2559
NcoI	CCATGG	1 cut(s)	585
NdeI	CATATG	1 cut(s)	2225
NlaIV	GGNNCC	8 cut(s)	618, 753, 872, 1499, 1566, 2294, 2375, 2619
NmuCI	GTSAC	5 cut(s)	78, 833, 1080, 1922, 2646
NsiI	ATGCAT	2 cut(s)	541, 545
NspI	RCATGY	4 cut(s)	543, 954, 2084, 2209
PaeI	GCATGC	3 cut(s)	543, 2084, 2209
PaeR7I	CTCGAG	1 cut(s)	188
PceI	AGGCCT	2 cut(s)	379, 1582
PciI	ACATGT	1 cut(s)	950
PctI	GAATGC	2 cut(s)	1736, 2342
PdmI	GAANNNNTTC	2 cut(s)	54, 578
PfeI	GAWTC	6 cut(s)	395, 934, 1175, 1304, 1945, 2275
PflFI	GACNNNGTC	1 cut(s)	485
PflMI	CCANNNNNTGG	1 cut(s)	794
PfoI	TCCNGGA	1 cut(s)	607
PkrI	GCNGC	5 cut(s)	214, 371, 1546, 1549, 2261
PleI	GAGTC	6 cut(s)	1184, 1828, 1929, 2408, 2434, 2510
PpsI	GAGTC	6 cut(s)	1184, 1828, 1929, 2408, 2434, 2510
PscI	ACATGT	1 cut(s)	950
PshAI	GACNNNNGTC	1 cut(s)	76
PshBI	ATTAAT	1 cut(s)	2127
Psp6I	CCWGG	3 cut(s)	95, 1846, 1916
PspGI	CCWGG	3 cut(s)	95, 1846, 1916
PspN4I	GGNNCC	8 cut(s)	618, 753, 872, 1499, 1566, 2294, 2375, 2619
PspPI	GGNCC	5 cut(s)	1497, 1672, 2106, 2293, 2560
PstNI	CAGNNNCTG	1 cut(s)	212
PsuI	RGATCY	5 cut(s)	751, 784, 1234, 1810, 2096
PsyI	GACNNNGTC	1 cut(s)	485
RsaI	GTAC	6 cut(s)	910, 1137, 1566, 1711, 2482, 2719
RsaNI	GTAC	6 cut(s)	909, 1136, 1565, 1710, 2481, 2718
RseI	CAYNNNNRTG	3 cut(s)	258, 2085, 2210
SatI	GCNGC	5 cut(s)	213, 370, 1545, 1548, 2260
Sau96I	GGNCC	5 cut(s)	1497, 1672, 2106, 2293, 2560
ScaI	AGTACT	2 cut(s)	910, 2719
SchI	GAGTC	6 cut(s)	1184, 1829, 1930, 2408, 2435, 2510
ScrFI	CCNGG	6 cut(s)	97, 468, 609, 1848, 1918, 2559
SduI	GDGCHC	3 cut(s)	621, 964, 1114
SfaNI	GCATC	4 cut(s)	26, 899, 1456, 1868
SfcI	CTRYAG	1 cut(s)	1311
Sfr274I	CTCGAG	1 cut(s)	188
SinI	GGWCC	3 cut(s)	1672, 2106, 2293
SlaI	CTCGAG	1 cut(s)	188
SmiMI	CAYNNNNRTG	3 cut(s)	258, 2085, 2210
SmlI	CTYRAG	6 cut(s)	153, 188, 692, 1169, 2170, 2416
SmoI	CTYRAG	6 cut(s)	153, 188, 692, 1169, 2170, 2416
SphI	GCATGC	3 cut(s)	543, 2084, 2209
SseBI	AGGCCT	2 cut(s)	379, 1582
SsiI	CCGC	4 cut(s)	370, 463, 519, 2484
SspI	AATATT	2 cut(s)	319, 1540
StuI	AGGCCT	2 cut(s)	379, 1582
StyD4I	CCNGG	6 cut(s)	95, 466, 607, 1846, 1916, 2557
StyI	CCWWGG	5 cut(s)	585, 1180, 1568, 2563, 2770
TaqI	TCGA	5 cut(s)	189, 450, 1307, 1341, 1597
TatI	WGTACW	2 cut(s)	908, 2717
TauI	GCSGC	1 cut(s)	372
TfiI	GAWTC	6 cut(s)	395, 934, 1175, 1304, 1945, 2275
TscAI	CASTG	6 cut(s)	146, 642, 932, 1114, 1254, 1644
TseFI	GTSAC	5 cut(s)	78, 833, 1080, 1922, 2646
TseI	GCWGC	4 cut(s)	212, 1544, 1547, 2259
Tsp45I	GTSAC	5 cut(s)	78, 833, 1080, 1922, 2646
TspDTI	ATGAA	9 cut(s)	47, 246, 272, 276, 309, 1242, 1277, 2292, 2678
TspGWI	ACGGA	2 cut(s)	289, 2098
TspRI	CASTG	6 cut(s)	146, 642, 932, 1114, 1254, 1644
Tth111I	GACNNNGTC	1 cut(s)	485
Van91I	CCANNNNNTGG	1 cut(s)	794
Vha464I	CTTAAG	1 cut(s)	1169
VpaK11BI	GGWCC	3 cut(s)	1672, 2106, 2293
VspI	ATTAAT	1 cut(s)	2127
XagI	CCTNNNNNAGG	1 cut(s)	1184
XceI	RCATGY	4 cut(s)	543, 954, 2084, 2209
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2250
XhoI	CTCGAG	1 cut(s)	188
XmaJI	CCTAGG	1 cut(s)	2563
XmnI	GAANNNNTTC	2 cut(s)	54, 578
ZrmI	AGTACT	2 cut(s)	910, 2719
Zsp2I	ATGCAT	2 cut(s)	541, 545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.