FvH4_4g03860

ENTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019693
Physical Location & Seq
Forward (+)
3385324 .. 3386314
991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03860.t1

Sequence Viewer

Length: 630 bp
ATGTCTAGCGCTAGAAAAACCTTAATCGCGTGTAGAGTGTGTGACAGTGTGTTCCTGAGCACCAAAGCCCTCATAAACCATTTAGATTCTCACTTGGTGGATGAGGAAGCTACTGCAAGAATCAGAAAACAACTTCAGTTAAGTCACCTCATGTCTTCTCAAAAATCTAATCCATTTTCGAACCCTCAGATCCAACCTGCTCCGGCTACTGCTCCTCTTCCTGTCCCTCCCTCACCACAAATCCAGCCTTATTTCAACCACAACAAGCAGTTCTACCCGCCAGTTCAAGTGCCACCACTCCCACCAGTGCCATATTTACAAAGAAACTCATTTGATATTCTACAAAATCCAAACCCAAACCTATATCCAAATCCATATCCTAATACAAAGGCTTATAATTCTTCACCGGCAAGAGATTTGAACGCAGGATTTGGATCTGGATTTGGGAAACACTCTTCGGTGCCATTTTCCGGACAAAGAACACAGCGTATGGCAGCAACGGAGGATTCTCGTGATAATTCAAGGCCGTTTCTGAGTCTCTTGGATTATCCTATTCCGCAAATGATGAACAGCAATTGTAACGGAAACTGCTCAGATGATTATCAGTTGGATTTGACCTTGAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ENTH

Protein Analysis

210

Amino Acids

23.28

Weight (kDa)

8.91

Isoelectric Point (pI)

69.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g03860
prunus_persica Prupe.1G040800_v2.0.a1
rosa_laevigata RLG00000009735
rosa_multiflora Rmu_sc0000928.1_g000001
rosa_roxburghii Rroxscaffold_5G00338880
rosa_rugosa Rorug03G0371400
rosa_samantha Rh4DG045800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
Acc36I ACCTGC 1 cut(s) 205
AccB1I GGYRCC 1 cut(s) 460
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 470
AciI CCGC 2 cut(s) 278, 557
AclWI GGATC 2 cut(s) 184, 442
AcuI CTGAAG 1 cut(s) 119
AdeI CACNNNGTG 1 cut(s) 97
AfeI AGCGCT 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 5 cut(s) 256, 287, 421, 522, 622
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 2 cut(s) 184, 442
Aor13HI TCCGGA 1 cut(s) 470
Aor51HI AGCGCT 1 cut(s) 10
AoxI GGCC 1 cut(s) 524
ApeKI GCWGC 1 cut(s) 494
AspLEI GCGC 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 137, 225, 396
AsuII TTCGAA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 460
BauI CACGAG 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 506
BceAI ACGGC 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 2 cut(s) 6, 12
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 1 cut(s) 495
BlsI GCNGC 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 147
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 179
BsaBI GATNNNNATC 2 cut(s) 433, 600
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 2 cut(s) 281, 305
Bse8I GATNNNNATC 2 cut(s) 433, 600
BseAI TCCGGA 1 cut(s) 470
BseGI GGATG 1 cut(s) 106
BseJI GATNNNNATC 2 cut(s) 433, 600
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 4 cut(s) 47, 200, 524, 606
BseNI ACTGG 2 cut(s) 281, 305
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 526
BshNI GGYRCC 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 3 cut(s) 203, 407, 471
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 1 cut(s) 526
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 62
Bsp13I TCCGGA 1 cut(s) 470
Bsp143I GATC 2 cut(s) 189, 434
BspACI CCGC 2 cut(s) 278, 557
BspANI GGCC 1 cut(s) 526
BspCNI CTCAG 4 cut(s) 48, 199, 525, 605
BspEI TCCGGA 1 cut(s) 470
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 462
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 2 cut(s) 184, 442
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 2 cut(s) 281, 305
BssAI RCCGGY 1 cut(s) 406
BssMI GATC 2 cut(s) 189, 434
BssSI CACGAG 1 cut(s) 510
Bst2BI CACGAG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 2 cut(s) 222, 460
BstBI TTCGAA 1 cut(s) 179
BstDEI CTNAG 4 cut(s) 56, 186, 533, 592
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 2 cut(s) 192, 437
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 2 cut(s) 189, 434
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 506
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 189, 434
BstYI RGATCY 2 cut(s) 189, 434
BsuRI GGCC 1 cut(s) 526
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 52, 312
BveI ACCTGC 1 cut(s) 205
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 406
CviAII CATG 1 cut(s) 151
CviJI RGCY 6 cut(s) 68, 110, 206, 247, 392, 526
CviKI_1 RGCY 6 cut(s) 68, 110, 206, 247, 392, 526
DdeI CTNAG 4 cut(s) 56, 186, 533, 592
DpnI GATC 2 cut(s) 191, 436
DpnII GATC 2 cut(s) 189, 434
DraIII CACNNNGTG 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 222, 460
EarI CTCTTC 2 cut(s) 222, 460
Eco47III AGCGCT 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 119
FaeI CATG 1 cut(s) 154
FaiI YATR 7 cut(s) 74, 152, 313, 364, 376, 396, 491
FaqI GGGAC 1 cut(s) 209
FatI CATG 1 cut(s) 150
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 495
FspBI CTAG 2 cut(s) 6, 12
GlaI GCGC 1 cut(s) 10
GluI GCNGC 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 526
HapII CCGG 3 cut(s) 203, 407, 471
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 4 cut(s) 86, 120, 506, 535
HpaII CCGG 3 cut(s) 203, 407, 471
HphI GGTGA 3 cut(s) 137, 225, 396
Hpy188I TCNGA 4 cut(s) 125, 189, 534, 595
Hpy188III TCNNGA 4 cut(s) 55, 438, 471, 512
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4V TGCA 1 cut(s) 116
HpyF3I CTNAG 4 cut(s) 56, 186, 533, 592
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 1 cut(s) 9
Kpn2I TCCGGA 1 cut(s) 470
Kzo9I GATC 2 cut(s) 189, 434
LmnI GCTCC 2 cut(s) 205, 217
Lsp1109I GCAGC 1 cut(s) 506
MaeI CTAG 2 cut(s) 6, 12
MaeIII GTNAC 3 cut(s) 41, 143, 578
MalI GATC 2 cut(s) 191, 436
MboI GATC 2 cut(s) 189, 434
MboII GAAGA 4 cut(s) 147, 209, 393, 447
MfeI CAATTG 1 cut(s) 574
MflI RGATCY 2 cut(s) 189, 434
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 3 cut(s) 397, 517, 574
MlyI GAGTC 1 cut(s) 544
MmeI TCCRAC 2 cut(s) 217, 588
MnlI CCTC 8 cut(s) 80, 97, 158, 195, 225, 237, 241, 496
MroI TCCGGA 1 cut(s) 470
MseI TTAA 2 cut(s) 23, 140
MspI CCGG 3 cut(s) 203, 407, 471
MunI CAATTG 1 cut(s) 574
MvnI CGCG 1 cut(s) 29
NdeII GATC 2 cut(s) 189, 434
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 2 cut(s) 41, 143
NspV TTCGAA 1 cut(s) 179
PfeI GAWTC 3 cut(s) 86, 120, 506
PkrI GCNGC 1 cut(s) 496
PleI GAGTC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 543
PsiI TTATAA 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 462
PsuI RGATCY 2 cut(s) 189, 434
SaqAI TTAA 2 cut(s) 23, 140
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 2 cut(s) 189, 434
SchI GAGTC 1 cut(s) 544
SduI GDGCHC 1 cut(s) 62
SetI ASST 6 cut(s) 23, 112, 150, 199, 363, 620
SfuI TTCGAA 1 cut(s) 179
Sse9I AATT 3 cut(s) 397, 517, 574
SsiI CCGC 2 cut(s) 278, 557
SspMI CTAG 2 cut(s) 6, 12
TaaI ACNGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 179
TasI AATT 3 cut(s) 397, 517, 574
TfiI GAWTC 3 cut(s) 86, 120, 506
Tru1I TTAA 2 cut(s) 23, 140
Tru9I TTAA 2 cut(s) 23, 140
TscAI CASTG 2 cut(s) 52, 312
TseFI GTSAC 2 cut(s) 41, 143
TseI GCWGC 1 cut(s) 494
Tsp45I GTSAC 2 cut(s) 41, 143
TspDTI ATGAA 1 cut(s) 581
TspGWI ACGGA 2 cut(s) 515, 597
TspRI CASTG 2 cut(s) 52, 312
XspI CTAG 2 cut(s) 6, 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.