Rorug03G0371400

ENTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0019693
Physical Location & Seq
Reverse (-)
45528195 .. 45530223
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0371400.1

Sequence Viewer

Length: 1566 bp
ATGGCTCTGTTTCTGTTCTTGAATCAAATGTGTGAAGAGCTGCAATCGAGCACTCTCTTCTATGTTTTCTTGATTTCTATTCTCTTTCTTTGTTCATTCATCTTATTTTCACAAATTAGATCGGGTGAGAAACTCAAATCTCCTCCATCCCCAACAAAGTTACCTTTAATCGGAAACCTTCACCAACTAGGCAAAATCCCACACCGGTCTCTTCAGGCCCTCTCAAAGAAGTATGGCTCTCTAATGCTACTGCACTTGGGCCAAGTTCCGACACTAGTAGTTTCATCTGCTGACATGGCCAAGGAAATAATGACGAAGCATGATGCAGTTTTCTCTGGGAGGCCCGAATCTATAGCTACAAATATATTAGCCTATGGATGCCAGAACATAGCCTTTTCTCCTTATGGAGAGTACTGGAAAAAAGTTCGGAAAGTTTGTGCTGTTGAACTTCTGAGCCTGAAAAGAGTGCAACAATTTCAGTATACAAGGGAAGAAGAAGTTGCAGAATTGGTTAGGAAGATACGCAAAGAGTGCTTCAGTGAGTCTCCTATTGATCTAAGTGACCTGCTGAGAAAAACCTCCAACAACATCCTGTCTAGGTGTGTTATTGGAAAAAGATTTATAGAGGAAAATGATAATTGGTTCGAAAAGGCATCAAGAAGTTGGTTGATTCAACTAACTTCTTTCAGTTTCGGAGATTACTTCCCTGGTTTGAGATGGATTGACTATCTTAGAGGATTCATTGCGCGTTTGAAAGCAACTTTTGCTGAATTAGATCAATTCTTTGATCAGTTGGTTGAATATCATAAGACTACAGGGGGAGAATATAAACTCAAAACAAGAGATTTTGTGGATATTCTCCTTAAATATGGAAAGGATAACGAGCTTAACTCGGAGCTCACTCGAGATGACATAAAAGCAATCCTACAGGACATGTTTGTTGGTGGAAGTGATACAACATCAACAACTTTGGAATGGTTAATGGCAGAGCTTGTGAGAAATCCACATGTGATGGAGAAGGTCCAACAAGAGGTAAGAAGAGTGGTAGGACAAAAGGCAAAGGTAGATGTGGATGATATCAATCAAATGGACTACTTAAAATGTGTCATCAAAGAAACTCTAAGACTACATCCTCCAGGAGCTCTTGTACCACGAGAAACAATCGCAGGTGTAACACTGGGAGGTTACTATATACCAGAGAAAACAAGAGTTTTTGTGAATATATTTGCCATCCAAAGGGACCCAAAGCTCTGGGACAAGCCAGATGAGTTCCTTCCAGACCGTTTCATGGATGACTCAATTAATTTCAAACCCCAGGATTTCCAATTTTTCCCGTTTGGTGGTGGGAGAAGACGCTGCCCCGGACAGGATTTTGCACATGCAGTAAATGAATATGTGATTGCCAATCTTCTGTATTGGTTTGATTGGAAGTTGCCAAGTGGTAATCTATCGGCCGAGGCATTAGACATGAGTGAACTCAATGGAATTGCTATCATGAAGAAATCTCCTGTTCTCCTTGTCCCCATACCATACTCCCTTAATCGTCCTCTATCCACAACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ENTH

Protein Analysis

521

Amino Acids

59.79

Weight (kDa)

8.24

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 48 - 482 1.5e-107 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g03860
prunus_persica Prupe.1G040800_v2.0.a1
rosa_laevigata RLG00000009735
rosa_multiflora Rmu_sc0000928.1_g000001
rosa_roxburghii Rroxscaffold_5G00338880
rosa_rugosa Rorug03G0371400
rosa_samantha Rh4DG045800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1157
Acc36I ACCTGC 2 cut(s) 573, 1157
AccI GTMKAC 1 cut(s) 482
AccII CGCG 1 cut(s) 748
AcoI YGGCCR 2 cut(s) 297, 1452
AcuI CTGAAG 2 cut(s) 197, 520
AfaI GTAC 2 cut(s) 413, 1149
AfiI CCNNNNNNNGG 6 cut(s) 170, 1030, 1236, 1288, 1340, 1366
AflIII ACRYGT 2 cut(s) 933, 1006
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 6 cut(s) 22, 446, 674, 754, 800, 1309
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 3 cut(s) 706, 1135, 1314
AluBI AGCT 7 cut(s) 40, 356, 886, 898, 991, 1142, 1249
AluI AGCT 7 cut(s) 40, 356, 886, 898, 991, 1142, 1249
Alw21I GWGCWC 3 cut(s) 53, 900, 1144
Alw26I GTCTC 2 cut(s) 213, 549
Ama87I CYCGRG 1 cut(s) 903
AoxI GGCC 5 cut(s) 216, 259, 297, 341, 1452
ApeKI GCWGC 2 cut(s) 40, 1356
AseI ATTAAT 1 cut(s) 1302
AsiGI ACCGGT 1 cut(s) 204
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 5 cut(s) 217, 259, 342, 1021, 1240
AsuC2I CCSGG 1 cut(s) 1362
AsuHPI GGTGA 2 cut(s) 137, 173
AsuII TTCGAA 1 cut(s) 645
AvaI CYCGRG 1 cut(s) 903
AvaII GGWCC 2 cut(s) 1021, 1240
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 2 cut(s) 900, 1144
BauI CACGAG 1 cut(s) 1152
BbsI GAAGAC 1 cut(s) 1357
Bbv12I GWGCWC 3 cut(s) 53, 900, 1144
BbvI GCAGC 2 cut(s) 27, 1343
BccI CCATC 4 cut(s) 154, 711, 1006, 1238
BciT130I CCWGG 3 cut(s) 708, 1137, 1316
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 1362
BcoDI GTCTC 2 cut(s) 213, 549
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 3 cut(s) 188, 275, 597
BfmI CTRYAG 3 cut(s) 351, 813, 926
BfuAI ACCTGC 2 cut(s) 573, 1157
BisI GCNGC 2 cut(s) 41, 1357
BlsI GCNGC 2 cut(s) 42, 1358
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 4 cut(s) 708, 1137, 1316, 1362
Bme18I GGWCC 2 cut(s) 1021, 1240
BmeT110I CYCGRG 1 cut(s) 903
BmgT120I GGNCC 5 cut(s) 217, 259, 342, 1021, 1240
BmiI GGNNCC 2 cut(s) 1241, 1242
BmrFI CCNGG 4 cut(s) 708, 1137, 1316, 1362
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 3 cut(s) 313, 368, 662
BmuI ACTGGG 1 cut(s) 1187
BpiI GAAGAC 1 cut(s) 1357
BplI GAGNNNNNCTC 2 cut(s) 875, 907
BpmI CTGGAG 1 cut(s) 1119
Bpu14I TTCGAA 1 cut(s) 645
BpuMI CCSGG 1 cut(s) 1362
BsaBI GATNNNNATC 1 cut(s) 1080
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 5 cut(s) 300, 706, 1314, 1360, 1455
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bsc4I CCNNNNNNNGG 6 cut(s) 170, 1030, 1236, 1288, 1340, 1366
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 2 cut(s) 419, 1182
Bse3DI GCAATG 1 cut(s) 741
Bse8I GATNNNNATC 1 cut(s) 1080
BseBI CCWGG 3 cut(s) 708, 1137, 1316
BseDI CCNNGG 5 cut(s) 300, 706, 1314, 1360, 1455
BseGI GGATG 7 cut(s) 146, 383, 588, 1078, 1129, 1230, 1297
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 6 cut(s) 170, 1030, 1236, 1288, 1340, 1366
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 443, 560
BseNI ACTGG 2 cut(s) 419, 1182
BseRI GAGGAG 1 cut(s) 132
BseX3I CGGCCG 1 cut(s) 1452
BseXI GCAGC 2 cut(s) 27, 1343
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 748
Bsh1285I CGRYCG 1 cut(s) 1455
BshFI GGCC 5 cut(s) 218, 261, 299, 343, 1454
BshTI ACCGGT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 1455
BsiHKAI GWGCWC 3 cut(s) 53, 900, 1144
BsiHKCI CYCGRG 1 cut(s) 903
BsiSI CCGG 2 cut(s) 205, 1362
BslFI GGGAC 3 cut(s) 1253, 1268, 1505
BslI CCNNNNNNNGG 6 cut(s) 170, 1030, 1236, 1288, 1340, 1366
BsmAI GTCTC 2 cut(s) 213, 549
BsmFI GGGAC 3 cut(s) 1253, 1268, 1505
BsnI GGCC 5 cut(s) 218, 261, 299, 343, 1454
Bso31I GGTCTC 1 cut(s) 213
BsoBI CYCGRG 1 cut(s) 903
Bsp119I TTCGAA 1 cut(s) 645
Bsp1286I GDGCHC 3 cut(s) 53, 900, 1144
Bsp143I GATC 4 cut(s) 119, 553, 775, 787
BspANI GGCC 5 cut(s) 218, 261, 299, 343, 1454
BspCNI CTCAG 2 cut(s) 444, 561
BspFNI CGCG 1 cut(s) 748
BspHI TCATGA 2 cut(s) 1494, 1562
BspLI GGNNCC 2 cut(s) 1241, 1242
BspMI ACCTGC 2 cut(s) 573, 1157
BspQI GCTCTTC 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 645
BspTNI GGTCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 2 cut(s) 419, 1182
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 5 cut(s) 300, 706, 1314, 1360, 1455
BssMI GATC 4 cut(s) 119, 553, 775, 787
BssNAI GTATAC 1 cut(s) 483
BssSI CACGAG 1 cut(s) 1152
BssT1I CCWWGG 1 cut(s) 300
Bst1107I GTATAC 1 cut(s) 483
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 3 cut(s) 708, 1137, 1316
Bst4CI ACNGT 1 cut(s) 1283
Bst6I CTCTTC 4 cut(s) 30, 62, 216, 1033
BstAPI GCANNNNNTGC 2 cut(s) 531, 764
BstBI TTCGAA 1 cut(s) 645
BstDEI CTNAG 5 cut(s) 452, 557, 569, 731, 1121
BstF5I GGATG 7 cut(s) 146, 383, 588, 1078, 1129, 1230, 1297
BstFNI CGCG 1 cut(s) 748
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 4 cut(s) 122, 556, 778, 790
BstMAI GTCTC 2 cut(s) 213, 549
BstMBI GATC 4 cut(s) 119, 553, 775, 787
BstMCI CGRYCG 1 cut(s) 1455
BstMWI GCNNNNNNNGC 3 cut(s) 296, 531, 764
BstNI CCWGG 3 cut(s) 708, 1137, 1316
BstNSI RCATGY 3 cut(s) 937, 1010, 1382
BstSCI CCNGG 4 cut(s) 706, 1135, 1314, 1360
BstSFI CTRYAG 3 cut(s) 351, 813, 926
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 2 cut(s) 27, 1343
BstV2I GAAGAC 1 cut(s) 1357
BstXI CCANNNNNNTGG 1 cut(s) 1251
BstZ17I GTATAC 1 cut(s) 483
BstZI CGGCCG 1 cut(s) 1452
BsuRI GGCC 5 cut(s) 218, 261, 299, 343, 1454
BtsCI GGATG 7 cut(s) 146, 383, 588, 1078, 1129, 1230, 1297
BtsIMutI CAGTG 2 cut(s) 544, 1175
BveI ACCTGC 2 cut(s) 573, 1157
CciI TCATGA 2 cut(s) 1494, 1562
CfoI GCGC 1 cut(s) 748
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 5 cut(s) 217, 259, 342, 1021, 1240
CseI GACGC 1 cut(s) 1362
Csp6I GTAC 2 cut(s) 412, 1148
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 9 cut(s) 295, 320, 934, 1007, 1288, 1379, 1468, 1495, 1563
CviQI GTAC 2 cut(s) 412, 1148
DdeI CTNAG 5 cut(s) 452, 557, 569, 731, 1121
DpnI GATC 4 cut(s) 121, 555, 777, 789
DpnII GATC 4 cut(s) 119, 553, 775, 787
EaeI YGGCCR 2 cut(s) 297, 1452
EagI CGGCCG 1 cut(s) 1452
Eam1104I CTCTTC 4 cut(s) 30, 62, 216, 1033
EarI CTCTTC 4 cut(s) 30, 62, 216, 1033
Ecl136II GAGCTC 2 cut(s) 898, 1142
EclXI CGGCCG 1 cut(s) 1452
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 2 cut(s) 900, 1144
Eco31I GGTCTC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 2 cut(s) 1021, 1240
Eco52I CGGCCG 1 cut(s) 1452
Eco53kI GAGCTC 2 cut(s) 898, 1142
Eco57I CTGAAG 2 cut(s) 197, 520
Eco88I CYCGRG 1 cut(s) 903
EcoICRI GAGCTC 2 cut(s) 898, 1142
EcoO109I RGGNCCY 2 cut(s) 217, 1240
EcoRII CCWGG 3 cut(s) 706, 1135, 1314
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 900, 1144
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 9 cut(s) 298, 323, 937, 1010, 1291, 1382, 1471, 1498, 1566
FaqI GGGAC 3 cut(s) 1253, 1268, 1505
FatI CATG 9 cut(s) 294, 319, 933, 1006, 1287, 1378, 1467, 1494, 1562
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 2 cut(s) 41, 1357
FokI GGATG 7 cut(s) 133, 390, 575, 1085, 1116, 1217, 1304
FriOI GRGCYC 2 cut(s) 900, 1144
Fsp4HI GCNGC 2 cut(s) 41, 1357
FspBI CTAG 3 cut(s) 188, 275, 597
GlaI GCGC 1 cut(s) 747
GluI GCNGC 2 cut(s) 41, 1357
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 5 cut(s) 218, 261, 299, 343, 1454
HapII CCGG 2 cut(s) 205, 1362
HgaI GACGC 1 cut(s) 1362
HhaI GCGC 1 cut(s) 748
Hin1II CATG 9 cut(s) 298, 323, 937, 1010, 1291, 1382, 1471, 1498, 1566
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HinfI GANTC 6 cut(s) 22, 347, 542, 670, 738, 1295
HpaII CCGG 2 cut(s) 205, 1362
HphI GGTGA 2 cut(s) 137, 173
Hpy166II GTNNAC 2 cut(s) 483, 1475
Hpy188I TCNGA 6 cut(s) 173, 270, 429, 453, 695, 895
Hpy188III TCNNGA 7 cut(s) 19, 70, 657, 905, 1277, 1495, 1563
Hpy8I GTNNAC 2 cut(s) 483, 1475
HpyAV CCTTC 3 cut(s) 188, 1012, 1283
HpyCH4III ACNGT 1 cut(s) 1283
HpyCH4V TGCA 7 cut(s) 43, 253, 326, 469, 503, 1376, 1382
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 531, 764
HpyF3I CTNAG 5 cut(s) 452, 557, 569, 731, 1121
Hsp92II CATG 9 cut(s) 298, 323, 937, 1010, 1291, 1382, 1471, 1498, 1566
HspAI GCGC 1 cut(s) 746
KflI GGGWCCC 1 cut(s) 1240
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 4 cut(s) 119, 553, 775, 787
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 2 cut(s) 895, 1139
Lsp1109I GCAGC 2 cut(s) 27, 1343
LweI GCATC 3 cut(s) 313, 368, 662
MaeI CTAG 3 cut(s) 188, 275, 597
MaeIII GTNAC 4 cut(s) 159, 560, 1171, 1184
MalI GATC 4 cut(s) 121, 555, 777, 789
MboI GATC 4 cut(s) 119, 553, 775, 787
MhlI GDGCHC 3 cut(s) 53, 900, 1144
MlsI TGGCCA 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 2 cut(s) 551, 1289
MmeI TCCRAC 3 cut(s) 293, 606, 1048
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 7 cut(s) 167, 864, 888, 980, 1097, 1302, 1539
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 2 cut(s) 205, 1362
MspR9I CCNGG 4 cut(s) 708, 1137, 1316, 1362
MvaI CCWGG 3 cut(s) 708, 1137, 1316
MvnI CGCG 1 cut(s) 748
MwoI GCNNNNNNNGC 3 cut(s) 296, 531, 764
NciI CCSGG 1 cut(s) 1362
NdeII GATC 4 cut(s) 119, 553, 775, 787
NlaIII CATG 9 cut(s) 298, 323, 937, 1010, 1291, 1382, 1471, 1498, 1566
NlaIV GGNNCC 2 cut(s) 1241, 1242
NmeAIII GCCGAG 1 cut(s) 1480
NmuCI GTSAC 1 cut(s) 560
NspI RCATGY 3 cut(s) 937, 1010, 1382
NspV TTCGAA 1 cut(s) 645
PaeR7I CTCGAG 1 cut(s) 903
PagI TCATGA 2 cut(s) 1494, 1562
PaqCI CACCTGC 1 cut(s) 1157
PciI ACATGT 2 cut(s) 933, 1006
PciSI GCTCTTC 1 cut(s) 30
PfeI GAWTC 4 cut(s) 22, 347, 670, 738
PfoI TCCNGGA 1 cut(s) 1135
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 2 cut(s) 42, 1358
PleI GAGTC 2 cut(s) 550, 1289
PpsI GAGTC 2 cut(s) 550, 1289
PpuMI RGGWCCY 1 cut(s) 1240
PscI ACATGT 2 cut(s) 933, 1006
PshBI ATTAAT 1 cut(s) 1302
Psp124BI GAGCTC 2 cut(s) 900, 1144
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 3 cut(s) 706, 1135, 1314
PspGI CCWGG 3 cut(s) 706, 1135, 1314
PspN4I GGNNCC 2 cut(s) 1241, 1242
PspPI GGNCC 5 cut(s) 217, 259, 342, 1021, 1240
PspPPI RGGWCCY 1 cut(s) 1240
RsaI GTAC 2 cut(s) 413, 1149
RsaNI GTAC 2 cut(s) 412, 1148
SacI GAGCTC 2 cut(s) 900, 1144
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 7 cut(s) 167, 864, 888, 980, 1097, 1302, 1539
SatI GCNGC 2 cut(s) 41, 1357
Sau3AI GATC 4 cut(s) 119, 553, 775, 787
Sau96I GGNCC 5 cut(s) 217, 259, 342, 1021, 1240
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 2 cut(s) 551, 1289
ScrFI CCNGG 4 cut(s) 708, 1137, 1316, 1362
SduI GDGCHC 3 cut(s) 53, 900, 1144
SfaNI GCATC 3 cut(s) 313, 368, 662
SfcI CTRYAG 3 cut(s) 351, 813, 926
Sfr274I CTCGAG 1 cut(s) 903
SfuI TTCGAA 1 cut(s) 645
SinI GGWCC 2 cut(s) 1021, 1240
SlaI CTCGAG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 903
SmoI CTYRAG 1 cut(s) 903
SpeI ACTAGT 1 cut(s) 274
SspMI CTAG 3 cut(s) 188, 275, 597
SstI GAGCTC 2 cut(s) 900, 1144
StyD4I CCNGG 4 cut(s) 706, 1135, 1314, 1360
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 1283
TaqI TCGA 3 cut(s) 47, 645, 904
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 4 cut(s) 22, 347, 670, 738
Tru1I TTAA 7 cut(s) 167, 864, 888, 980, 1097, 1302, 1539
Tru9I TTAA 7 cut(s) 167, 864, 888, 980, 1097, 1302, 1539
TscAI CASTG 2 cut(s) 544, 1182
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 2 cut(s) 40, 1356
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 8 cut(s) 84, 88, 273, 730, 1276, 1404, 1511, 1551
TspRI CASTG 2 cut(s) 544, 1182
VpaK11BI GGWCC 2 cut(s) 1021, 1240
VspI ATTAAT 1 cut(s) 1302
XceI RCATGY 3 cut(s) 937, 1010, 1382
XhoI CTCGAG 1 cut(s) 903
XmiI GTMKAC 1 cut(s) 482
XspI CTAG 3 cut(s) 188, 275, 597
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.