FvH4_4g04390

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017281
Physical Location & Seq
Reverse (-)
3759919 .. 3760755
837 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04390.t1

Sequence Viewer

Length: 837 bp
ATGGCATCACTAGCCACCCATTTCTCAGCCTTCGTGCTTCTCTCCCCGGTTGGCCTCCGCCGCCTGCTCTGCTCCTCCTCCCTTTACCTTAAAAACCCTTCACTTTTCCGCGCCAAAACTTGGTACTTCTCCGACCCTAAATGGAAAAACATCGATCTCTACGCCCTCCTCATCGCCCTCCCCATCGCCTCCTTCTCCGAAATCTTCATCTTCCTCGCCTTCTCCGGCCACCCCACTTACCGCTTCGCCTTCTTCCAGCAGTCCGCCACCATCTTCTTCTTCTGGCTTCTCCTCCTCCTCATCATCCTCCGTGAAAACCTAGACTACCCCATGCACATCGACCAGGCCTTTATCTTCGTCTTGGCCGCAGTCACGTTCTTCGTTGAATATTCCGTCATCGGTAAAGGTATCTCCGGCTTGGGAGGAACCATGTACGACATGTTAGGTGTACTCACTCTTGCATGCGCCTTTTCTTGCTTGTACTTGTCGTTCCGGCCTAGGTCGTTTTTCGCAGAGTTTTTCTTGTCGTCCGGTTTGGTTTTTAAGGGTACGTGGATGTTGCAGCTAGGCTTGTCGTTGTACACTGACACGTTTGGGTTGAAAGGGTGTAAGAAGATGGCTGTGTGGCCTAAGGAGTCTGAGAATGCTGAGTTTAAGTGTGATCTTGAGGAGGATGGGTTGAGGGGTGTGGCTGTGATGACGTTGTTGTTTATAGGGCATGCGATTGGGATAATGATGTCGAGCTTTGTGATGTTTGGCTGGCTTGGAAGGAGCTTGAACTGTTGGTGCGGCGAGGCGAGTGGACCGTTGTTGCAAGAGCTGGAATCGGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

279

Amino Acids

31.35

Weight (kDa)

6.42

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 117 - 252 3.2e-23 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04390
malus_domestica MD13G1219500.v1.1 MD16G1225000.v1.1
prunus_persica Prupe.1G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394961
rosa_laevigata RLG00000009655
rosa_multiflora Rmu_sc0005802.1_g000027
rosa_roxburghii Rroxscaffold_5G00339930
rosa_rugosa Rorug03G0360800.1
rosa_samantha Rh4BG054700 Rh4CG060600 Rh4DG052000
rosa_wichuraiana Rw4G004470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AccII CGCG 1 cut(s) 111
AciI CCGC 7 cut(s) 58, 61, 109, 241, 264, 366, 789
AcoI YGGCCR 2 cut(s) 226, 363
AfaI GTAC 6 cut(s) 125, 434, 450, 482, 550, 581
AfiI CCNNNNNNNGG 1 cut(s) 120
AflIII ACRYGT 2 cut(s) 438, 588
AgsI TTSAA 3 cut(s) 386, 601, 778
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 4 cut(s) 565, 744, 774, 820
AluI AGCT 4 cut(s) 565, 744, 774, 820
AoxI GGCC 6 cut(s) 52, 226, 345, 363, 494, 626
ApeKI GCWGC 1 cut(s) 562
AspA2I CCTAGG 1 cut(s) 497
AspLEI GCGC 2 cut(s) 113, 467
AspS9I GGNCC 1 cut(s) 803
AsuC2I CCSGG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 803
AvrII CCTAGG 1 cut(s) 497
AxyI CCTNAGG 1 cut(s) 630
BbvI GCAGC 1 cut(s) 574
BccI CCATC 4 cut(s) 191, 278, 610, 668
BciT130I CCWGG 1 cut(s) 344
BcnI CCSGG 1 cut(s) 47
BfaI CTAG 4 cut(s) 11, 320, 498, 566
BisI GCNGC 4 cut(s) 61, 366, 563, 790
BlnI CCTAGG 1 cut(s) 497
BlsI GCNGC 4 cut(s) 62, 367, 564, 791
Bme1390I CCNGG 2 cut(s) 47, 344
Bme18I GGWCC 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 803
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 2 cut(s) 47, 344
BmsI GCATC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 45, 497
BsaWI WCCGGW 1 cut(s) 530
BsaXI ACNNNNNCTCC 2 cut(s) 662, 692
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse21I CCTNAGG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 344
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 45, 497
BseGI GGATG 3 cut(s) 303, 561, 679
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 39, 630, 639
BseRI GAGGAG 7 cut(s) 64, 67, 158, 281, 284, 287, 683
BseXI GCAGC 1 cut(s) 574
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 6 cut(s) 54, 228, 347, 365, 496, 628
BshVI ATCGAT 1 cut(s) 153
BsiSI CCGG 5 cut(s) 47, 225, 414, 493, 531
BslI CCNNNNNNNGG 1 cut(s) 120
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 6 cut(s) 54, 228, 347, 365, 496, 628
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 2 cut(s) 154, 661
BspACI CCGC 7 cut(s) 58, 61, 109, 241, 264, 366, 789
BspANI GGCC 6 cut(s) 54, 228, 347, 365, 496, 628
BspCNI CTCAG 3 cut(s) 38, 631, 640
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 427
BsrGI TGTACA 1 cut(s) 579
BssECI CCNNGG 2 cut(s) 45, 497
BssMI GATC 2 cut(s) 154, 661
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 782, 807
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 552
BstC8I GCNNGC 4 cut(s) 65, 463, 720, 761
BstDEI CTNAG 4 cut(s) 25, 630, 639, 648
BstF5I GGATG 3 cut(s) 303, 561, 679
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 2 cut(s) 113, 467
BstKTI GATC 2 cut(s) 157, 664
BstMBI GATC 2 cut(s) 154, 661
BstMWI GCNNNNNNNGC 3 cut(s) 11, 60, 69
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 3 cut(s) 442, 465, 722
BstSCI CCNGG 2 cut(s) 45, 342
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 574
Bsu15I ATCGAT 1 cut(s) 153
Bsu36I CCTNAGG 1 cut(s) 630
BsuRI GGCC 6 cut(s) 54, 228, 347, 365, 496, 628
BsuTUI ATCGAT 1 cut(s) 153
BtgZI GCGATG 2 cut(s) 157, 169
BtsCI GGATG 3 cut(s) 303, 561, 679
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 4 cut(s) 65, 463, 720, 761
CfoI GCGC 2 cut(s) 113, 467
Cfr13I GGNCC 1 cut(s) 803
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 6 cut(s) 124, 433, 449, 481, 549, 580
CviAII CATG 5 cut(s) 331, 430, 439, 462, 719
CviQI GTAC 6 cut(s) 124, 433, 449, 481, 549, 580
DdeI CTNAG 4 cut(s) 25, 630, 639, 648
DpnI GATC 2 cut(s) 156, 663
DpnII GATC 2 cut(s) 154, 661
EaeI YGGCCR 2 cut(s) 226, 363
EciI GGCGGA 2 cut(s) 47, 253
Eco130I CCWWGG 1 cut(s) 497
Eco147I AGGCCT 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 803
Eco81I CCTNAGG 1 cut(s) 630
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 5 cut(s) 334, 433, 442, 465, 722
FaiI YATR 6 cut(s) 332, 431, 440, 463, 713, 720
FatI CATG 5 cut(s) 330, 429, 438, 461, 718
Fnu4HI GCNGC 4 cut(s) 61, 366, 563, 790
FokI GGATG 3 cut(s) 290, 568, 686
Fsp4HI GCNGC 4 cut(s) 61, 366, 563, 790
FspBI CTAG 4 cut(s) 11, 320, 498, 566
GlaI GCGC 2 cut(s) 112, 466
GluI GCNGC 4 cut(s) 61, 366, 563, 790
HaeIII GGCC 6 cut(s) 54, 228, 347, 365, 496, 628
HapII CCGG 5 cut(s) 47, 225, 414, 493, 531
HhaI GCGC 2 cut(s) 113, 467
Hin1II CATG 5 cut(s) 334, 433, 442, 465, 722
Hin6I GCGC 2 cut(s) 111, 465
HinP1I GCGC 2 cut(s) 111, 465
HinfI GANTC 2 cut(s) 635, 824
HpaII CCGG 5 cut(s) 47, 225, 414, 493, 531
Hpy166II GTNNAC 3 cut(s) 449, 582, 803
Hpy188I TCNGA 4 cut(s) 133, 199, 640, 829
Hpy188III TCNNGA 1 cut(s) 665
Hpy8I GTNNAC 3 cut(s) 449, 582, 803
HpyAV CCTTC 6 cut(s) 40, 108, 202, 229, 259, 762
HpyCH4III ACNGT 2 cut(s) 782, 807
HpyCH4IV ACGT 4 cut(s) 374, 551, 590, 701
HpyCH4V TGCA 4 cut(s) 334, 461, 562, 814
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 60, 69
HpyF3I CTNAG 4 cut(s) 25, 630, 639, 648
HpySE526I ACGT 4 cut(s) 374, 551, 590, 701
Hsp92II CATG 5 cut(s) 334, 433, 442, 465, 722
HspAI GCGC 2 cut(s) 111, 465
Kzo9I GATC 2 cut(s) 154, 661
LmnI GCTCC 3 cut(s) 77, 771, 829
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 1 cut(s) 14
MaeI CTAG 4 cut(s) 11, 320, 498, 566
MaeII ACGT 4 cut(s) 374, 551, 590, 701
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 156, 663
MboI GATC 2 cut(s) 154, 661
MboII GAAGA 9 cut(s) 196, 202, 244, 265, 268, 271, 346, 370, 625
MlyI GAGTC 1 cut(s) 644
MmeI TCCRAC 1 cut(s) 156
MseI TTAA 3 cut(s) 90, 543, 654
MspI CCGG 5 cut(s) 47, 225, 414, 493, 531
MspR9I CCNGG 2 cut(s) 47, 344
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 3 cut(s) 11, 60, 69
NciI CCSGG 1 cut(s) 47
NdeII GATC 2 cut(s) 154, 661
NlaIII CATG 5 cut(s) 334, 433, 442, 465, 722
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 1 cut(s) 370
NspI RCATGY 3 cut(s) 442, 465, 722
PaeI GCATGC 2 cut(s) 465, 722
PceI AGGCCT 1 cut(s) 347
PciI ACATGT 1 cut(s) 438
PctI GAATGC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 824
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 4 cut(s) 62, 367, 564, 791
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
Ppu21I YACGTR 1 cut(s) 552
PscI ACATGT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 1 cut(s) 803
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
RsaI GTAC 6 cut(s) 125, 434, 450, 482, 550, 581
RsaNI GTAC 6 cut(s) 124, 433, 449, 481, 549, 580
SaqAI TTAA 3 cut(s) 90, 543, 654
SatI GCNGC 4 cut(s) 61, 366, 563, 790
Sau3AI GATC 2 cut(s) 154, 661
Sau96I GGNCC 1 cut(s) 803
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 2 cut(s) 47, 344
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 1 cut(s) 803
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SphI GCATGC 2 cut(s) 465, 722
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 7 cut(s) 58, 61, 109, 241, 264, 366, 789
SspI AATATT 1 cut(s) 389
SspMI CTAG 4 cut(s) 11, 320, 498, 566
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 2 cut(s) 45, 342
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 2 cut(s) 782, 807
TaiI ACGT 4 cut(s) 377, 554, 593, 704
TaqI TCGA 3 cut(s) 153, 339, 740
TatI WGTACW 3 cut(s) 448, 480, 579
TauI GCSGC 3 cut(s) 63, 368, 792
TfiI GAWTC 1 cut(s) 824
Tru1I TTAA 3 cut(s) 90, 543, 654
Tru9I TTAA 3 cut(s) 90, 543, 654
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 1 cut(s) 196
TspGWI ACGGA 2 cut(s) 299, 382
TspRI CASTG 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 803
XceI RCATGY 3 cut(s) 442, 465, 722
XmaJI CCTAGG 1 cut(s) 497
XspI CTAG 4 cut(s) 11, 320, 498, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.