Rroxscaffold_5G00339930

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017281
Physical Location & Seq
Reverse (-)
8332985 .. 8333863
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00339930.1

Sequence Viewer

Length: 879 bp
ATGGCATCACTAGCAACCCATTTCTCAGCGTTCGTGCTTCTCTCCCCGGTGGGCCTCCGCCGCCTGCTCTGCTCCTCCTCCCTGTACCTGAGAAACCCATCTCAGTTCCGCGCCAAAACTTGGTACTTCTCCGACCCCAAATGGAAAAACTTCGATCTCTACTCTCTCATCGTCGCCCTCCCCATCGCCTCCTTCTCCGAGATCTTCCTCTTCCTCGCCTTCTCCGGCCACCCCACCTACCGCTTCGCCTTCTTCCAGCAGTCCGCCACCATCTTCCTCTTCTGGCTTCTCCTCCTTTTGATCATCTGCCGCGAAAACATAGACCCCATGCACATCGACGAGGGCTTCGTCTTCGTCTTGGCCGCGATCACGTTCTTCATCGAGTATTCCGTCATCGGAAAAGGTATCTCCGGTTTGGGTGGGACCATGTACGACATGTCGGGTGCACTCACTCTCGCATGTGCCTTTTCTTGCTTGTACTTGTCGTTCCGCCCGTGGTCGTTTTTCGCCGAGTTTTTCTTGTCGTCCGGGTTGGTTTTTAAAGGTACGTGGATGTTGCAGCTAGGGTTGTCATTGTACACTGACACGTTTGGGCTGAAAGGGTGTAAGAAGATGGCTGTGTGGCCCCATAAGTCTGAGAATGCCGAGTTGAAGTGTGATCTTGAGGAGGATGGGTTGAGGGGTTTGGCTGTGATGACATTGTTGTTTATTGGGCATGCAATTGGGGTAATGGTGTTGAGTTTTGTGTTGTTTGGGTGGCTAGCAAGAAACTGGAACTGGCGGTCCGGCGAGGCTAGTGGACCGTTGCTGCAACAGCTGGAATCGGAGCACCAACTTGTGTTGATGCGTGCTACGAGTCAAGAGATTGAGCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

292

Amino Acids

33.05

Weight (kDa)

6.31

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 115 - 251 2.1e-21 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04390
malus_domestica MD13G1219500.v1.1 MD16G1225000.v1.1
prunus_persica Prupe.1G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394961
rosa_laevigata RLG00000009655
rosa_multiflora Rmu_sc0005802.1_g000027
rosa_roxburghii Rroxscaffold_5G00339930
rosa_rugosa Rorug03G0360800.1
rosa_samantha Rh4BG054700 Rh4CG060600 Rh4DG052000
rosa_wichuraiana Rw4G004470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AccII CGCG 3 cut(s) 111, 312, 365
AciI CCGC 9 cut(s) 58, 61, 109, 241, 264, 310, 363, 490, 781
AcoI YGGCCR 2 cut(s) 226, 360
AfaI GTAC 6 cut(s) 86, 125, 431, 479, 547, 578
AfiI CCNNNNNNNGG 1 cut(s) 120
AflIII ACRYGT 2 cut(s) 435, 585
AgsI TTSAA 1 cut(s) 652
AloI GAACNNNNNNTCC 2 cut(s) 767, 799
AluBI AGCT 3 cut(s) 562, 817, 871
AluI AGCT 3 cut(s) 562, 817, 871
Alw21I GWGCWC 2 cut(s) 448, 831
Alw44I GTGCAC 1 cut(s) 444
AoxI GGCC 4 cut(s) 52, 226, 360, 623
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 559, 808
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 5 cut(s) 52, 423, 624, 783, 800
AsuC2I CCSGG 2 cut(s) 47, 529
AsuNHI GCTAGC 1 cut(s) 760
AvaII GGWCC 3 cut(s) 423, 783, 800
BaeGI GKGCMC 1 cut(s) 448
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 2 cut(s) 448, 831
BbvI GCAGC 2 cut(s) 571, 795
BccI CCATC 5 cut(s) 106, 191, 278, 607, 665
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 2 cut(s) 47, 529
BfaI CTAG 4 cut(s) 11, 563, 761, 795
BglII AGATCT 1 cut(s) 201
BisI GCNGC 5 cut(s) 61, 310, 363, 560, 809
BlsI GCNGC 5 cut(s) 62, 311, 364, 561, 810
Bme1390I CCNGG 2 cut(s) 47, 529
Bme18I GGWCC 3 cut(s) 423, 783, 800
BmgT120I GGNCC 5 cut(s) 52, 423, 624, 783, 800
BmiI GGNNCC 2 cut(s) 424, 626
BmrFI CCNGG 2 cut(s) 47, 529
BmsI GCATC 2 cut(s) 14, 834
BmtI GCTAGC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 343
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 2 cut(s) 47, 529
BsaAI YACGTR 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 45, 494
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 776, 782
BseDI CCNNGG 2 cut(s) 45, 494
BseGI GGATG 2 cut(s) 558, 676
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMII CTCAG 4 cut(s) 39, 80, 116, 627
BseNI ACTGG 2 cut(s) 776, 782
BseRI GAGGAG 4 cut(s) 64, 67, 281, 680
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 2 cut(s) 571, 795
Bsh1236I CGCG 3 cut(s) 111, 312, 365
BshFI GGCC 4 cut(s) 54, 228, 362, 625
BsiHKAI GWGCWC 2 cut(s) 448, 831
BsiSI CCGG 5 cut(s) 47, 225, 411, 528, 786
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 54, 228, 362, 625
Bsp1286I GDGCHC 2 cut(s) 448, 831
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 5 cut(s) 154, 201, 300, 366, 658
BspACI CCGC 9 cut(s) 58, 61, 109, 241, 264, 310, 363, 490, 781
BspANI GGCC 4 cut(s) 54, 228, 362, 625
BspCNI CTCAG 4 cut(s) 38, 81, 115, 628
BspFNI CGCG 3 cut(s) 111, 312, 365
BspLI GGNNCC 2 cut(s) 424, 626
BspOI GCTAGC 1 cut(s) 764
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 2 cut(s) 776, 782
BssECI CCNNGG 2 cut(s) 45, 494
BssMI GATC 5 cut(s) 154, 201, 300, 366, 658
Bst4CI ACNGT 1 cut(s) 804
Bst6I CTCTTC 2 cut(s) 215, 284
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 549
BstC8I GCNNGC 4 cut(s) 65, 717, 762, 849
BstDEI CTNAG 4 cut(s) 25, 89, 102, 636
BstDSI CCRYGG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 558, 676
BstFNI CGCG 3 cut(s) 111, 312, 365
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 5 cut(s) 157, 204, 303, 369, 661
BstMBI GATC 5 cut(s) 154, 201, 300, 366, 658
BstMWI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 814
BstNSI RCATGY 3 cut(s) 439, 462, 719
BstSCI CCNGG 2 cut(s) 45, 527
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 3 cut(s) 111, 312, 365
BstV1I GCAGC 2 cut(s) 571, 795
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BsuRI GGCC 4 cut(s) 54, 228, 362, 625
BtgI CCRYGG 1 cut(s) 494
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 2 cut(s) 558, 676
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 4 cut(s) 65, 717, 762, 849
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 5 cut(s) 52, 423, 624, 783, 800
CpoI CGGWCCG 1 cut(s) 783
Csp6I GTAC 6 cut(s) 85, 124, 430, 478, 546, 577
CspI CGGWCCG 1 cut(s) 783
CviAII CATG 5 cut(s) 328, 427, 436, 459, 716
CviQI GTAC 6 cut(s) 85, 124, 430, 478, 546, 577
DdeI CTNAG 4 cut(s) 25, 89, 102, 636
DpnI GATC 5 cut(s) 156, 203, 302, 368, 660
DpnII GATC 5 cut(s) 154, 201, 300, 366, 658
DraI TTTAAA 1 cut(s) 541
EaeI YGGCCR 2 cut(s) 226, 360
Eam1104I CTCTTC 2 cut(s) 215, 284
EarI CTCTTC 2 cut(s) 215, 284
EciI GGCGGA 3 cut(s) 47, 253, 479
Eco47I GGWCC 3 cut(s) 423, 783, 800
FaeI CATG 5 cut(s) 331, 430, 439, 462, 719
FaiI YATR 7 cut(s) 320, 329, 428, 437, 460, 630, 717
FaqI GGGAC 1 cut(s) 436
FatI CATG 5 cut(s) 327, 426, 435, 458, 715
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 61, 310, 363, 560, 809
FokI GGATG 2 cut(s) 565, 683
Fsp4HI GCNGC 5 cut(s) 61, 310, 363, 560, 809
FspBI CTAG 4 cut(s) 11, 563, 761, 795
GlaI GCGC 1 cut(s) 112
GluI GCNGC 5 cut(s) 61, 310, 363, 560, 809
HaeIII GGCC 4 cut(s) 54, 228, 362, 625
HapII CCGG 5 cut(s) 47, 225, 411, 528, 786
HhaI GCGC 1 cut(s) 113
Hin1II CATG 5 cut(s) 331, 430, 439, 462, 719
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HinfI GANTC 2 cut(s) 821, 856
HpaII CCGG 5 cut(s) 47, 225, 411, 528, 786
Hpy166II GTNNAC 3 cut(s) 446, 579, 800
Hpy188I TCNGA 5 cut(s) 133, 199, 398, 637, 826
Hpy188III TCNNGA 2 cut(s) 662, 860
Hpy8I GTNNAC 3 cut(s) 446, 579, 800
Hpy99I CGWCG 2 cut(s) 176, 341
HpyAV CCTTC 3 cut(s) 202, 229, 259
HpyCH4III ACNGT 1 cut(s) 804
HpyCH4IV ACGT 3 cut(s) 371, 548, 587
HpyCH4V TGCA 5 cut(s) 331, 446, 559, 719, 811
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 814
HpyF3I CTNAG 4 cut(s) 25, 89, 102, 636
HpySE526I ACGT 3 cut(s) 371, 548, 587
Hsp92II CATG 5 cut(s) 331, 430, 439, 462, 719
HspAI GCGC 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 5 cut(s) 154, 201, 300, 366, 658
LmnI GCTCC 2 cut(s) 77, 826
Lsp1109I GCAGC 2 cut(s) 571, 795
LweI GCATC 2 cut(s) 14, 834
MaeI CTAG 4 cut(s) 11, 563, 761, 795
MaeII ACGT 3 cut(s) 371, 548, 587
MalI GATC 5 cut(s) 156, 203, 302, 368, 660
MboI GATC 5 cut(s) 154, 201, 300, 366, 658
MboII GAAGA 8 cut(s) 196, 202, 244, 265, 271, 343, 367, 622
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 448, 831
MluCI AATT 1 cut(s) 720
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 5 cut(s) 47, 225, 411, 528, 786
MspR9I CCNGG 2 cut(s) 47, 529
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 1 cut(s) 646
MvnI CGCG 3 cut(s) 111, 312, 365
MwoI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 814
NciI CCSGG 2 cut(s) 47, 529
NdeII GATC 5 cut(s) 154, 201, 300, 366, 658
NheI GCTAGC 1 cut(s) 760
NlaIII CATG 5 cut(s) 331, 430, 439, 462, 719
NlaIV GGNNCC 2 cut(s) 424, 626
NmeAIII GCCGAG 2 cut(s) 535, 670
NspI RCATGY 3 cut(s) 439, 462, 719
PaeI GCATGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 3 cut(s) 345, 387, 491
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 821
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 5 cut(s) 62, 311, 364, 561, 810
PleI GAGTC 1 cut(s) 864
PpsI GAGTC 1 cut(s) 864
Ppu21I YACGTR 1 cut(s) 549
PscI ACATGT 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 424, 626
PspPI GGNCC 5 cut(s) 52, 423, 624, 783, 800
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PsuI RGATCY 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 6 cut(s) 86, 125, 431, 479, 547, 578
RsaNI GTAC 6 cut(s) 85, 124, 430, 478, 546, 577
Rsr2I CGGWCCG 1 cut(s) 783
RsrII CGGWCCG 1 cut(s) 783
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 5 cut(s) 61, 310, 363, 560, 809
Sau3AI GATC 5 cut(s) 154, 201, 300, 366, 658
Sau96I GGNCC 5 cut(s) 52, 423, 624, 783, 800
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 2 cut(s) 47, 529
SduI GDGCHC 2 cut(s) 448, 831
SfaNI GCATC 2 cut(s) 14, 834
SinI GGWCC 3 cut(s) 423, 783, 800
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SphI GCATGC 1 cut(s) 719
Sse9I AATT 1 cut(s) 720
SsiI CCGC 9 cut(s) 58, 61, 109, 241, 264, 310, 363, 490, 781
SspMI CTAG 4 cut(s) 11, 563, 761, 795
StyD4I CCNGG 2 cut(s) 45, 527
TaaI ACNGT 1 cut(s) 804
TaiI ACGT 3 cut(s) 374, 551, 590
TaqI TCGA 3 cut(s) 153, 336, 381
TasI AATT 1 cut(s) 720
TatI WGTACW 2 cut(s) 477, 576
TauI GCSGC 3 cut(s) 63, 312, 365
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 586
TseI GCWGC 2 cut(s) 559, 808
TspDTI ATGAA 1 cut(s) 367
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 120
VneI GTGCAC 1 cut(s) 444
VpaK11BI GGWCC 3 cut(s) 423, 783, 800
XceI RCATGY 3 cut(s) 439, 462, 719
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 4 cut(s) 11, 563, 761, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.