MD13G1219500.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017281
Physical Location & Seq
Reverse (-)
21206676 .. 21207539
864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1219500.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGCATCACTAGCAACCCATTTCTCCGCCTTCGTCCTCCTCTTTCCCATAGGCCTCCGCCGCTTCATCTGCTCCTCCTCCCTCTACCTCAAGAACCCATCTCTCTACCAATCCAAAGCATGGTACTGCTCAGAAACCAAATGGAGAAACTTCGATTTGTACTCTCTCACCATCGCCCTCCCCATCGCCTGCTTCTGCGAAATCTTCCTCTTTCTGGCCTTTTCCGGCCACCACCCCACCTACCGCTTCGGCTTCTTCCAGCAATCCGCCGCTATTTTCATGTTCTGGGCACTCGTACTTCTGATCATCTTCCGTGAAAACATCGACCCTTTGAATTTCAATGAAGGGTTCGTCTTTGTTTTCGCAGCGGTTTCGTTTTTCGTTGAGTACTCTGTGATCGGCAAGGGAATTTCGGGTCTTGGTGGCGCTGTGTATGATATGCTAGGTGCATTGACTCTAGTTTGTGCATTTTCTTGCCTGTATTTGTCGATTAGGCCCTGTTCGTATTTCGCCGAGTTTTTCTTGTCCTCTGGATTGGTTTTTAAGGGCACTTGGATTTTGCAACTGGGTTTGTCCTTGTACACTGATGTATTTGGGTTAAAAGGGTGTAAGAAAATGTCGGTTTGGCCTAACCAGGACAGTGCTGAGTGGAAATGTGACCTTGAGGAGGACGCTTTGAGGGGTTTCGCTGTGATGAAGCTCTTGTTCATCGGGCATGCGATTGCGATCCTGGTATTGAGTTTTGTGTTGTTTGCTTTGTTGGCAAGTAACTGCAACTTGCAGGCTGGCGAGGCCAGTGGCCCGTTGCTAGCGCAGCTGGAGCATGTATTGATTCATCCTGGCCCCGAGATTGAGCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

288

Amino Acids

32.08

Weight (kDa)

5.87

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 116 - 252 1.3e-20 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04390
malus_domestica MD13G1219500.v1.1 MD16G1225000.v1.1
prunus_persica Prupe.1G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394961
rosa_laevigata RLG00000009655
rosa_multiflora Rmu_sc0005802.1_g000027
rosa_roxburghii Rroxscaffold_5G00339930
rosa_rugosa Rorug03G0360800.1
rosa_samantha Rh4BG054700 Rh4CG060600 Rh4DG052000
rosa_wichuraiana Rw4G004470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AciI CCGC 7 cut(s) 27, 58, 61, 244, 267, 270, 368
AclWI GGATC 1 cut(s) 721
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 334, 408
AfaI GTAC 5 cut(s) 125, 161, 297, 389, 581
AfiI CCNNNNNNNGG 3 cut(s) 120, 214, 667
AgsI TTSAA 2 cut(s) 334, 340
AjnI CCWGG 3 cut(s) 633, 729, 838
AluBI AGCT 3 cut(s) 700, 817, 856
AluI AGCT 3 cut(s) 700, 817, 856
AlwI GGATC 1 cut(s) 721
Ama87I CYCGRG 1 cut(s) 845
AoxI GGCC 8 cut(s) 52, 216, 226, 494, 626, 792, 799, 841
ApeKI GCWGC 2 cut(s) 365, 814
ApoI RAATTY 2 cut(s) 334, 408
AspLEI GCGC 2 cut(s) 428, 814
AspS9I GGNCC 3 cut(s) 495, 800, 842
AsuHPI GGTGA 1 cut(s) 160
AsuNHI GCTAGC 1 cut(s) 808
AvaI CYCGRG 1 cut(s) 845
BaeGI GKGCMC 2 cut(s) 292, 551
BbvI GCAGC 2 cut(s) 377, 826
BccI CCATC 3 cut(s) 106, 179, 191
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BciT130I CCWGG 3 cut(s) 635, 731, 840
BclI TGATCA 1 cut(s) 303
BfaI CTAG 4 cut(s) 11, 443, 458, 809
BfoI RGCGCY 1 cut(s) 429
BisI GCNGC 4 cut(s) 61, 270, 366, 815
BlsI GCNGC 4 cut(s) 62, 271, 367, 816
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 3 cut(s) 635, 731, 840
BmeT110I CYCGRG 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 495, 800, 842
BmiI GGNNCC 1 cut(s) 844
BmrFI CCNGG 3 cut(s) 635, 731, 840
BmrI ACTGGG 1 cut(s) 575
BmsI GCATC 1 cut(s) 14
BmtI GCTAGC 1 cut(s) 812
BmuI ACTGGG 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 839
BpuEI CTTGAG 2 cut(s) 74, 683
BsaBI GATNNNNATC 1 cut(s) 725
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 214, 667
Bse1I ACTGG 2 cut(s) 570, 795
Bse8I GATNNNNATC 1 cut(s) 725
BseBI CCWGG 3 cut(s) 635, 731, 840
BseGI GGATG 1 cut(s) 835
BseJI GATNNNNATC 1 cut(s) 725
BseLI CCNNNNNNNGG 3 cut(s) 120, 214, 667
BseMII CTCAG 2 cut(s) 144, 636
BseNI ACTGG 2 cut(s) 570, 795
BseRI GAGGAG 4 cut(s) 29, 64, 67, 680
BseSI GKGCMC 2 cut(s) 292, 551
BseXI GCAGC 2 cut(s) 377, 826
BshFI GGCC 8 cut(s) 54, 218, 228, 496, 628, 794, 801, 843
BsiHKCI CYCGRG 1 cut(s) 845
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 3 cut(s) 120, 214, 667
BsnI GGCC 8 cut(s) 54, 218, 228, 496, 628, 794, 801, 843
BsoBI CYCGRG 1 cut(s) 845
Bsp1286I GDGCHC 2 cut(s) 292, 551
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 3 cut(s) 303, 396, 726
BspACI CCGC 7 cut(s) 27, 58, 61, 244, 267, 270, 368
BspANI GGCC 8 cut(s) 54, 218, 228, 496, 628, 794, 801, 843
BspCNI CTCAG 2 cut(s) 143, 637
BspLI GGNNCC 1 cut(s) 844
BspOI GCTAGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 721
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 2 cut(s) 570, 795
BssMI GATC 3 cut(s) 303, 396, 726
Bst2UI CCWGG 3 cut(s) 635, 731, 840
Bst4CI ACNGT 1 cut(s) 641
BstAUI TGTACA 1 cut(s) 579
BstC8I GCNNGC 5 cut(s) 190, 717, 783, 787, 810
BstDEI CTNAG 2 cut(s) 130, 645
BstENI CCTNNNNNAGG 1 cut(s) 665
BstF5I GGATG 1 cut(s) 835
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 2 cut(s) 428, 814
BstKTI GATC 3 cut(s) 306, 399, 729
BstMBI GATC 3 cut(s) 303, 396, 726
BstMWI GCNNNNNNNGC 7 cut(s) 11, 60, 69, 761, 791, 814, 820
BstNI CCWGG 3 cut(s) 635, 731, 840
BstNSI RCATGY 2 cut(s) 719, 827
BstSCI CCNGG 3 cut(s) 633, 729, 838
BstSLI GKGCMC 2 cut(s) 292, 551
BstV1I GCAGC 2 cut(s) 377, 826
BsuRI GGCC 8 cut(s) 54, 218, 228, 496, 628, 794, 801, 843
BtgZI GCGATG 2 cut(s) 157, 169
BtsCI GGATG 1 cut(s) 835
BtsIMutI CAGTG 3 cut(s) 582, 646, 802
Cac8I GCNNGC 5 cut(s) 190, 717, 783, 787, 810
CfoI GCGC 2 cut(s) 428, 814
Cfr13I GGNCC 3 cut(s) 495, 800, 842
CseI GACGC 1 cut(s) 680
Csp6I GTAC 5 cut(s) 124, 160, 296, 388, 580
CviAII CATG 4 cut(s) 120, 280, 716, 824
CviQI GTAC 5 cut(s) 124, 160, 296, 388, 580
DdeI CTNAG 2 cut(s) 130, 645
DpnI GATC 3 cut(s) 305, 398, 728
DpnII GATC 3 cut(s) 303, 396, 726
EaeI YGGCCR 1 cut(s) 226
EciI GGCGGA 3 cut(s) 16, 47, 256
Eco147I AGGCCT 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 845
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoO109I RGGNCCY 1 cut(s) 495
EcoRII CCWGG 3 cut(s) 633, 729, 838
FaeI CATG 4 cut(s) 123, 283, 719, 827
FaiI YATR 7 cut(s) 50, 121, 281, 435, 440, 717, 825
FatI CATG 4 cut(s) 119, 279, 715, 823
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 61, 270, 366, 815
FokI GGATG 1 cut(s) 822
Fsp4HI GCNGC 4 cut(s) 61, 270, 366, 815
FspBI CTAG 4 cut(s) 11, 443, 458, 809
GlaI GCGC 2 cut(s) 427, 813
GluI GCNGC 4 cut(s) 61, 270, 366, 815
GsuI CTGGAG 1 cut(s) 839
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 8 cut(s) 54, 218, 228, 496, 628, 794, 801, 843
HapII CCGG 1 cut(s) 225
HgaI GACGC 1 cut(s) 680
HhaI GCGC 2 cut(s) 428, 814
Hin1II CATG 4 cut(s) 123, 283, 719, 827
Hin6I GCGC 2 cut(s) 426, 812
HinP1I GCGC 2 cut(s) 426, 812
HinfI GANTC 2 cut(s) 454, 832
HpaII CCGG 1 cut(s) 225
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 582
Hpy188I TCNGA 2 cut(s) 133, 303
Hpy188III TCNNGA 2 cut(s) 91, 531
Hpy8I GTNNAC 1 cut(s) 582
HpyAV CCTTC 2 cut(s) 40, 338
HpyCH4III ACNGT 1 cut(s) 641
HpyCH4V TGCA 5 cut(s) 449, 467, 562, 774, 781
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 60, 69, 761, 791, 814, 820
HpyF3I CTNAG 2 cut(s) 130, 645
Hsp92II CATG 4 cut(s) 123, 283, 719, 827
HspAI GCGC 2 cut(s) 426, 812
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 3 cut(s) 303, 396, 726
LmnI GCTCC 2 cut(s) 77, 820
Lsp1109I GCAGC 2 cut(s) 377, 826
LweI GCATC 1 cut(s) 14
MaeI CTAG 4 cut(s) 11, 443, 458, 809
MaeIII GTNAC 2 cut(s) 656, 767
MalI GATC 3 cut(s) 305, 398, 728
MboI GATC 3 cut(s) 303, 396, 726
MboII GAAGA 3 cut(s) 196, 247, 301
MhlI GDGCHC 2 cut(s) 292, 551
MluCI AATT 2 cut(s) 334, 408
MlyI GAGTC 1 cut(s) 448
MseI TTAA 2 cut(s) 543, 599
MspA1I CMGCKG 2 cut(s) 368, 817
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 3 cut(s) 635, 731, 840
MvaI CCWGG 3 cut(s) 635, 731, 840
MwoI GCNNNNNNNGC 7 cut(s) 11, 60, 69, 761, 791, 814, 820
NdeII GATC 3 cut(s) 303, 396, 726
NheI GCTAGC 1 cut(s) 808
NlaIII CATG 4 cut(s) 123, 283, 719, 827
NlaIV GGNNCC 1 cut(s) 844
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 656
NspI RCATGY 2 cut(s) 719, 827
PaeI GCATGC 1 cut(s) 719
PceI AGGCCT 1 cut(s) 54
PfeI GAWTC 1 cut(s) 832
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 4 cut(s) 62, 271, 367, 816
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Psp6I CCWGG 3 cut(s) 633, 729, 838
PspGI CCWGG 3 cut(s) 633, 729, 838
PspN4I GGNNCC 1 cut(s) 844
PspPI GGNCC 3 cut(s) 495, 800, 842
PvuII CAGCTG 1 cut(s) 817
RsaI GTAC 5 cut(s) 125, 161, 297, 389, 581
RsaNI GTAC 5 cut(s) 124, 160, 296, 388, 580
SaqAI TTAA 2 cut(s) 543, 599
SatI GCNGC 4 cut(s) 61, 270, 366, 815
Sau3AI GATC 3 cut(s) 303, 396, 726
Sau96I GGNCC 3 cut(s) 495, 800, 842
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 3 cut(s) 635, 731, 840
SduI GDGCHC 2 cut(s) 292, 551
SetI ASST 7 cut(s) 90, 242, 448, 663, 702, 819, 858
SfaNI GCATC 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 89, 662
SmoI CTYRAG 2 cut(s) 89, 662
SphI GCATGC 1 cut(s) 719
Sse9I AATT 2 cut(s) 334, 408
SseBI AGGCCT 1 cut(s) 54
SsiI CCGC 7 cut(s) 27, 58, 61, 244, 267, 270, 368
SspMI CTAG 4 cut(s) 11, 443, 458, 809
StuI AGGCCT 1 cut(s) 54
StyD4I CCNGG 3 cut(s) 633, 729, 838
TaaI ACNGT 1 cut(s) 641
TaqI TCGA 3 cut(s) 153, 324, 488
TasI AATT 2 cut(s) 334, 408
TatI WGTACW 3 cut(s) 159, 387, 579
TauI GCSGC 2 cut(s) 63, 272
TfiI GAWTC 1 cut(s) 832
Tru1I TTAA 2 cut(s) 543, 599
Tru9I TTAA 2 cut(s) 543, 599
TscAI CASTG 3 cut(s) 589, 646, 802
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 2 cut(s) 365, 814
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 6 cut(s) 55, 268, 357, 697, 710, 824
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 3 cut(s) 589, 646, 802
Van91I CCANNNNNTGG 1 cut(s) 120
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 2 cut(s) 334, 408
XceI RCATGY 2 cut(s) 719, 827
XspI CTAG 4 cut(s) 11, 443, 458, 809
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.