MD16G1225000.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017281
Physical Location & Seq
Reverse (-)
22706530 .. 22707396
867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1225000.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCATCACTAGCAACCCATTTCTCCGCCTTCGCCCTCCTCTTCCCCATAGGCCTCCGCCGCCTCATCTGCTCCTCCGCCCTCTACCTCAAGAACCCATCTCTCTACCGATCCAAAGCTTGGTACTTTTCAGAACCCAAATGGAAAAACTTCGATTTGTACTCTCTCACCATCGCCCTCCCCATCGCCTCCTTCTCCGAAATCTTCATCTTTCTGGCCTTTTCCGGCCACCCCACCTACCGCTTCGCCTTCTTCCAGCAATCAGCCGCCATTTTCATGTTCTGGGCACTCGTCCTTCTGATCATCTTCCGTGAAAACATCGACCCTTTGAATTTCAATGAAGGGTTCGTCTTCGTTTTTGCAGCGGTTTCCTTTTTCGTTGAGTACTCTGTGATCGGCAAGGGAATTTCGGGTCTTGGTGGCGCTGTGTATGATATGCTAGCTGCATTGACTCTAATTTGTGCATTTTCTTGCCTGTATTTGTCGATTAGGCCATGTTCGTTTTTCGCCGAGTTTTTCTTGTCCTCTGGATTGGTTTTTAAGGGCACTTGGATTTTGCAACTGGGGTTGTCCTTGTACACTGATGCATTTGGGTTAAACGGGTGTAAGAAAATGTCAGTGTGGCCTAACCAGGACAATGCTGAGTGGAAATGTAACCTTGAGGAGGACGGGTTAAGGGGTGTTGCTGTGATGAAGCTCTTGTTCATCGGGCATGCGATTGCGGTCTTGGTTGGCAAGGGCAGTGGTCAGTTGCTAGCACAGCTAGATTCGGAGCATGTGTTGATGCCTCCTGGCGGTGAGATTGGGCTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

272

Amino Acids

30.09

Weight (kDa)

6.43

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 115 - 244 1.3e-21 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017281)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04390
malus_domestica MD13G1219500.v1.1 MD16G1225000.v1.1
prunus_persica Prupe.1G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394961
rosa_laevigata RLG00000009655
rosa_multiflora Rmu_sc0005802.1_g000027
rosa_roxburghii Rroxscaffold_5G00339930
rosa_rugosa Rorug03G0360800.1
rosa_samantha Rh4BG054700 Rh4CG060600 Rh4DG052000
rosa_wichuraiana Rw4G004470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AciI CCGC 9 cut(s) 27, 58, 61, 78, 241, 267, 365, 722, 795
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 331, 405
AfaI GTAC 4 cut(s) 125, 161, 386, 578
AfiI CCNNNNNNNGG 3 cut(s) 120, 664, 794
AgsI TTSAA 3 cut(s) 331, 337, 812
AjnI CCWGG 2 cut(s) 630, 790
AluBI AGCT 4 cut(s) 119, 443, 697, 763
AluI AGCT 4 cut(s) 119, 443, 697, 763
AlwI GGATC 1 cut(s) 105
AoxI GGCC 5 cut(s) 52, 216, 226, 491, 623
ApeKI GCWGC 2 cut(s) 362, 443
ApoI RAATTY 2 cut(s) 331, 405
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 160, 809
AsuNHI GCTAGC 2 cut(s) 439, 754
BaeGI GKGCMC 2 cut(s) 289, 548
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 2 cut(s) 374, 430
BccI CCATC 3 cut(s) 106, 179, 191
BciT130I CCWGG 2 cut(s) 632, 792
BclI TGATCA 1 cut(s) 300
BfaI CTAG 4 cut(s) 11, 440, 755, 764
BfoI RGCGCY 1 cut(s) 426
BisI GCNGC 4 cut(s) 61, 267, 363, 444
BlsI GCNGC 4 cut(s) 62, 268, 364, 445
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 632, 792
BmrFI CCNGG 2 cut(s) 632, 792
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 3 cut(s) 14, 574, 774
BmtI GCTAGC 2 cut(s) 443, 758
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 1 cut(s) 343
BpuEI CTTGAG 2 cut(s) 74, 680
BsaXI ACNNNNNCTCC 4 cut(s) 656, 686, 764, 794
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 664, 794
Bse1I ACTGG 1 cut(s) 567
BseBI CCWGG 2 cut(s) 632, 792
BseLI CCNNNNNNNGG 3 cut(s) 120, 664, 794
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 567
BseRI GAGGAG 3 cut(s) 29, 64, 677
BseSI GKGCMC 2 cut(s) 289, 548
BseXI GCAGC 2 cut(s) 374, 430
BshFI GGCC 5 cut(s) 54, 218, 228, 493, 625
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 3 cut(s) 120, 664, 794
BsnI GGCC 5 cut(s) 54, 218, 228, 493, 625
Bsp1286I GDGCHC 2 cut(s) 289, 548
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 3 cut(s) 110, 300, 393
BspACI CCGC 9 cut(s) 27, 58, 61, 78, 241, 267, 365, 722, 795
BspANI GGCC 5 cut(s) 54, 218, 228, 493, 625
BspCNI CTCAG 1 cut(s) 634
BspOI GCTAGC 2 cut(s) 443, 758
BspPI GGATC 1 cut(s) 105
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 1 cut(s) 567
BssMI GATC 3 cut(s) 110, 300, 393
Bst2UI CCWGG 2 cut(s) 632, 792
Bst6I CTCTTC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 441, 714, 756
BstDEI CTNAG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 662
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 3 cut(s) 113, 303, 396
BstMBI GATC 3 cut(s) 110, 300, 393
BstMWI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 760
BstNI CCWGG 2 cut(s) 632, 792
BstNSI RCATGY 2 cut(s) 716, 779
BstSCI CCNGG 2 cut(s) 630, 790
BstSLI GKGCMC 2 cut(s) 289, 548
BstV1I GCAGC 2 cut(s) 374, 430
BstV2I GAAGAC 1 cut(s) 343
BsuRI GGCC 5 cut(s) 54, 218, 228, 493, 625
BtgZI GCGATG 2 cut(s) 157, 169
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 3 cut(s) 579, 624, 748
Cac8I GCNNGC 3 cut(s) 441, 714, 756
CfoI GCGC 1 cut(s) 425
Csp6I GTAC 4 cut(s) 124, 160, 385, 577
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviAII CATG 4 cut(s) 277, 495, 713, 776
CviQI GTAC 4 cut(s) 124, 160, 385, 577
DdeI CTNAG 1 cut(s) 642
DpnI GATC 3 cut(s) 112, 302, 395
DpnII GATC 3 cut(s) 110, 300, 393
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 3 cut(s) 16, 47, 67
Eco147I AGGCCT 1 cut(s) 54
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoRII CCWGG 2 cut(s) 630, 790
EcoT22I ATGCAT 1 cut(s) 589
FaeI CATG 4 cut(s) 280, 498, 716, 779
FaiI YATR 7 cut(s) 50, 278, 432, 437, 496, 714, 777
FatI CATG 4 cut(s) 276, 494, 712, 775
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 4 cut(s) 61, 267, 363, 444
Fsp4HI GCNGC 4 cut(s) 61, 267, 363, 444
FspBI CTAG 4 cut(s) 11, 440, 755, 764
GlaI GCGC 1 cut(s) 424
GluI GCNGC 4 cut(s) 61, 267, 363, 444
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 5 cut(s) 54, 218, 228, 493, 625
HapII CCGG 1 cut(s) 225
HhaI GCGC 1 cut(s) 425
Hin1II CATG 4 cut(s) 280, 498, 716, 779
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 2 cut(s) 451, 767
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 160, 809
Hpy166II GTNNAC 1 cut(s) 579
Hpy188I TCNGA 4 cut(s) 133, 199, 300, 772
Hpy188III TCNNGA 2 cut(s) 91, 528
Hpy8I GTNNAC 1 cut(s) 579
HpyAV CCTTC 5 cut(s) 40, 202, 259, 305, 335
HpyCH4V TGCA 5 cut(s) 362, 446, 464, 559, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 760
HpyF3I CTNAG 1 cut(s) 642
Hsp92II CATG 4 cut(s) 280, 498, 716, 779
HspAI GCGC 1 cut(s) 423
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 3 cut(s) 110, 300, 393
LmnI GCTCC 2 cut(s) 77, 772
Lsp1109I GCAGC 2 cut(s) 374, 430
LweI GCATC 3 cut(s) 14, 574, 774
MaeI CTAG 4 cut(s) 11, 440, 755, 764
MaeIII GTNAC 1 cut(s) 653
MalI GATC 3 cut(s) 112, 302, 395
MboI GATC 3 cut(s) 110, 300, 393
MboII GAAGA 5 cut(s) 34, 196, 244, 298, 343
MhlI GDGCHC 2 cut(s) 289, 548
MluCI AATT 3 cut(s) 331, 405, 456
MlyI GAGTC 1 cut(s) 445
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 3 cut(s) 540, 596, 674
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 2 cut(s) 632, 792
MvaI CCWGG 2 cut(s) 632, 792
MwoI GCNNNNNNNGC 4 cut(s) 11, 60, 69, 760
NdeII GATC 3 cut(s) 110, 300, 393
NheI GCTAGC 2 cut(s) 439, 754
NlaIII CATG 4 cut(s) 280, 498, 716, 779
NmeAIII GCCGAG 1 cut(s) 535
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 2 cut(s) 716, 779
PaeI GCATGC 1 cut(s) 716
PceI AGGCCT 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 767
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 4 cut(s) 62, 268, 364, 445
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
Psp6I CCWGG 2 cut(s) 630, 790
PspGI CCWGG 2 cut(s) 630, 790
RsaI GTAC 4 cut(s) 125, 161, 386, 578
RsaNI GTAC 4 cut(s) 124, 160, 385, 577
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 3 cut(s) 540, 596, 674
SatI GCNGC 4 cut(s) 61, 267, 363, 444
Sau3AI GATC 3 cut(s) 110, 300, 393
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 632, 792
SduI GDGCHC 2 cut(s) 289, 548
SetI ASST 7 cut(s) 90, 121, 239, 445, 660, 699, 765
SfaNI GCATC 3 cut(s) 14, 574, 774
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 89, 659
SmoI CTYRAG 2 cut(s) 89, 659
SphI GCATGC 1 cut(s) 716
Sse9I AATT 3 cut(s) 331, 405, 456
SseBI AGGCCT 1 cut(s) 54
SsiI CCGC 9 cut(s) 27, 58, 61, 78, 241, 267, 365, 722, 795
SspMI CTAG 4 cut(s) 11, 440, 755, 764
StuI AGGCCT 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 630, 790
TaqI TCGA 3 cut(s) 153, 321, 485
TasI AATT 3 cut(s) 331, 405, 456
TatI WGTACW 3 cut(s) 159, 384, 576
TauI GCSGC 2 cut(s) 63, 269
TfiI GAWTC 1 cut(s) 767
Tru1I TTAA 3 cut(s) 540, 596, 674
Tru9I TTAA 3 cut(s) 540, 596, 674
TscAI CASTG 3 cut(s) 586, 624, 748
TseI GCWGC 2 cut(s) 362, 443
TspDTI ATGAA 5 cut(s) 196, 265, 354, 694, 707
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 3 cut(s) 586, 624, 748
Van91I CCANNNNNTGG 1 cut(s) 120
XagI CCTNNNNNAGG 1 cut(s) 662
XapI RAATTY 2 cut(s) 331, 405
XceI RCATGY 2 cut(s) 716, 779
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 4 cut(s) 11, 440, 755, 764
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.