FvH4_4g05760

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010648
Physical Location & Seq
Reverse (-)
5130961 .. 5136618
5658 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05760.t1

Sequence Viewer

Length: 1356 bp
ATGGCGGTTTCGGAGAATAAGAAATGGATTACGGCCTGCGTGTCGGTGCTTCTTCTGTTCTGCTTCGTCGCTGCAATGTCCGGTCGTGTCAGCCTCGACGGTACCCCTATCCTCAGGAATGGTGGAACGGAGGAGTTGCAGAGCTCGAGCAATTCATCAATGGCGCCCAGGGTGAATGAGAATGATGATGGAGGGTTGAATAAGCATGCAGTTGAGAACCCAGATGAAATTGCTGCCATGGTTGACATGAGTATCCGCAACAGCACTGAAAGGAGAAAGCTGGGCTACTTCTCATGTGGAACTGGAAATCCCATTGATGATTGCTGGCGTTGTGATCCCAACTGGCAAAAGAACCGCAAGCGTCTTGCAGACTGTGGAATTGGTTTTGGCAGAAATGCCATTGGTGGTCGTGATGGACGCTTCTATGTTGTCACTGATCCTAATGATGATGATCCCGTTAACCCCAGACCCGGCACTCTGCGCCATGCTGTCATCCAGGATGAGCCCCTCTGGATTGTGTTCAAACGTGACATGGTGATCCAATTGAAGCAGGAGCTTATCATGAATAGCTTCAAGACCATTGATGGCCGTGGAGTCAATGTCCACATTGCTAATGGAGCATGCATTACAATCCAGTTTGTCACAAATGTTATAGTTCATGGTCTGCATATCCATGACTGCAAGCCCACAGGAAATGCTTTGGTGAGGAGCTCCCCATCTCACTTTGGATGGAGGACAATGGCTGATGGTGATGCTATCTCCATCTTCGGGTCAAGCCATATCTGGGTTGATCACAATTCCCTCTCCAATTGTGCTGATGGTCTTGTTGATGCTGTCATGGGGTCAACTGCTATTACCATCTCCAACAACCACCTTACCCACCACAATGAAGTAATGCTGTTGGGCCACAGCGATTCTTATACCAGGGACAAGCAAATGCAAGTGACTATTGCCTACAACCACTTTGGAGAGGGACTTATCCAGAGAATGCCAAGGTGCAGACATGGATATTTCCATGTGGTGAACAACGACTACACTCACTGGGAAATGTATGCCATCGGTGGAAGTGCAGACCCCACTATCAACAGCCAAGGCAACAGATATGCAGCTCCAACCAACCCCTTTGCAAAGGAGGTTACCAAGAGAGTGGAGACATCGCAAACTCAGTGGAGGGGCTGGAACTGGAGGTCAGAAGGTGATCTTCTACTCAATGGTGCCTTCTTCACTCCATCTGGAGCTGGAGCGTCAGCTGTCTATGCCAGGGCCTCAAGCTTGGGAGCCAAGTCATCGGCCATGGTGGGAACCATAACTGCTGGTGCTGGTGCACTTGGATGCCGCAGAGGACGTACCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

452

Amino Acids

49.36

Weight (kDa)

7.63

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 184 - 386 5e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24230 AT4G13710 AT4G13710
fragaria_vesca FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760
malus_domestica MD13G1231000.v1.1 MD16G1236000.v1.1
prunus_persica Prupe.1G060900_v2.0.a1
pyrus_communis pycom13g20360 pycom16g19790
rosa_chinensis RchiOBHm_Chr4g0396921
rosa_laevigata RLG00000009489
rosa_roxburghii Rroxscaffold_5G00341840
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075300 Rh4DG069100
rosa_wichuraiana Rw4G006040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 3 cut(s) 101, 163, 1214
AciI CCGC 4 cut(s) 5, 256, 355, 1336
AclWI GGATC 4 cut(s) 329, 431, 446, 532
AcoI YGGCCR 2 cut(s) 586, 1290
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 2 cut(s) 103, 1348
AfiI CCNNNNNNNGG 3 cut(s) 470, 768, 784
AgsI TTSAA 4 cut(s) 199, 523, 547, 574
AjnI CCWGG 4 cut(s) 167, 495, 923, 1259
AloI GAACNNNNNNTCC 2 cut(s) 292, 324
AluBI AGCT 9 cut(s) 144, 280, 556, 570, 711, 1109, 1238, 1250, 1272
AluI AGCT 9 cut(s) 144, 280, 556, 570, 711, 1109, 1238, 1250, 1272
Alw21I GWGCWC 3 cut(s) 146, 713, 1327
Alw26I GTCTC 1 cut(s) 1145
Alw44I GTGCAC 1 cut(s) 1323
AlwI GGATC 4 cut(s) 329, 431, 446, 532
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 5 cut(s) 33, 586, 904, 1263, 1290
ApaLI GTGCAC 1 cut(s) 1323
ApeKI GCWGC 3 cut(s) 71, 233, 1106
Asp700I GAANNNNTTC 1 cut(s) 569
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 166, 483
AspS9I GGNCC 2 cut(s) 904, 1263
AsuC2I CCSGG 1 cut(s) 471
AsuHPI GGTGA 6 cut(s) 184, 547, 715, 761, 1033, 1208
AvaI CYCGRG 1 cut(s) 145
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 1327
BaeI ACNNNNGTAYC 2 cut(s) 235, 268
BanI GGYRCC 3 cut(s) 101, 163, 1214
BanII GRGCYC 3 cut(s) 146, 507, 713
Bbv12I GWGCWC 3 cut(s) 146, 713, 1327
BbvI GCAGC 3 cut(s) 58, 220, 1118
BceAI ACGGC 2 cut(s) 48, 573
BciT130I CCWGG 4 cut(s) 169, 497, 925, 1261
BciVI GTATCC 1 cut(s) 263
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 1145
BfaI CTAG 1 cut(s) 1354
BfoI RGCGCY 1 cut(s) 167
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 4 cut(s) 72, 234, 1107, 1336
BlsI GCNGC 4 cut(s) 73, 235, 1108, 1337
Bme1390I CCNGG 5 cut(s) 169, 471, 497, 925, 1261
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 904, 1263
BmiI GGNNCC 5 cut(s) 103, 165, 1216, 1279, 1303
BmrFI CCNGG 5 cut(s) 169, 471, 497, 925, 1261
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 742, 820, 1322
BmuI ACTGGG 1 cut(s) 1051
BpmI CTGGAG 3 cut(s) 1204, 1254, 1260
BpuEI CTTGAG 1 cut(s) 1252
BpuMI CCSGG 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 9 cut(s) 167, 168, 237, 589, 924, 992, 1090, 1260, 1293
BsaWI WCCGGW 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 3 cut(s) 470, 768, 784
Bse1I ACTGG 5 cut(s) 307, 347, 634, 1046, 1187
Bse21I CCTNAGG 1 cut(s) 113
Bse3DI GCAATG 2 cut(s) 81, 606
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 4 cut(s) 169, 497, 925, 1261
BseDI CCNNGG 9 cut(s) 167, 168, 237, 589, 924, 992, 1090, 1260, 1293
BseGI GGATG 4 cut(s) 492, 505, 734, 1337
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 3 cut(s) 470, 768, 784
BseMI GCAATG 2 cut(s) 81, 606
BseMII CTCAG 2 cut(s) 127, 1178
BseNI ACTGG 5 cut(s) 307, 347, 634, 1046, 1187
BseRI GAGGAG 2 cut(s) 146, 721
BseSI GKGCMC 1 cut(s) 1327
BseXI GCAGC 3 cut(s) 58, 220, 1118
BseYI CCCAGC 1 cut(s) 280
BsgI GTGCAG 2 cut(s) 1018, 1089
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 5 cut(s) 35, 588, 906, 1265, 1292
BshNI GGYRCC 3 cut(s) 101, 163, 1214
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 3 cut(s) 146, 713, 1327
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 2 cut(s) 81, 471
BslFI GGGAC 2 cut(s) 941, 987
BslI CCNNNNNNNGG 3 cut(s) 470, 768, 784
BsmAI GTCTC 1 cut(s) 1145
BsmFI GGGAC 2 cut(s) 941, 987
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 5 cut(s) 35, 588, 906, 1265, 1292
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 4 cut(s) 146, 507, 713, 1327
Bsp143I GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
Bsp19I CCATGG 2 cut(s) 237, 1293
BspACI CCGC 4 cut(s) 5, 256, 355, 1336
BspANI GGCC 5 cut(s) 35, 588, 906, 1265, 1292
BspCNI CTCAG 2 cut(s) 126, 1177
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 5 cut(s) 103, 165, 1216, 1279, 1303
BspPI GGATC 4 cut(s) 329, 431, 446, 532
BspT107I GGYRCC 3 cut(s) 101, 163, 1214
BsrDI GCAATG 2 cut(s) 81, 606
BsrI ACTGG 5 cut(s) 307, 347, 634, 1046, 1187
BssECI CCNNGG 9 cut(s) 167, 168, 237, 589, 924, 992, 1090, 1260, 1293
BssMI GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 4 cut(s) 237, 992, 1090, 1293
Bst2UI CCWGG 4 cut(s) 169, 497, 925, 1261
Bst4CI ACNGT 2 cut(s) 101, 374
BstACI GRCGYC 1 cut(s) 164
BstC8I GCNNGC 6 cut(s) 37, 207, 326, 359, 622, 683
BstDEI CTNAG 2 cut(s) 113, 1164
BstDSI CCRYGG 3 cut(s) 237, 589, 1293
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 4 cut(s) 492, 505, 734, 1337
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 2 cut(s) 166, 483
BstKTI GATC 6 cut(s) 337, 439, 454, 540, 793, 1201
BstMAI GTCTC 1 cut(s) 1145
BstMBI GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 480, 617, 1256
BstNI CCWGG 4 cut(s) 169, 497, 925, 1261
BstNSI RCATGY 2 cut(s) 209, 624
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 5 cut(s) 167, 469, 495, 923, 1259
BstSLI GKGCMC 1 cut(s) 1327
BstV1I GCAGC 3 cut(s) 58, 220, 1118
BstXI CCANNNNNNTGG 1 cut(s) 1147
Bsu36I CCTNAGG 1 cut(s) 113
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 5 cut(s) 35, 588, 906, 1265, 1292
BtgI CCRYGG 3 cut(s) 237, 589, 1293
BtgZI GCGATG 1 cut(s) 1140
BtsCI GGATG 4 cut(s) 492, 505, 734, 1337
BtsIMutI CAGTG 4 cut(s) 264, 432, 1039, 1172
Cac8I GCNNGC 6 cut(s) 37, 207, 326, 359, 622, 683
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 166, 483
Cfr13I GGNCC 2 cut(s) 904, 1263
CseI GACGC 3 cut(s) 350, 426, 1233
Csp6I GTAC 2 cut(s) 102, 1347
CspCI CAANNNNNGTGG 2 cut(s) 1148, 1183
CviQI GTAC 2 cut(s) 102, 1347
DdeI CTNAG 2 cut(s) 113, 1164
DinI GGCGCC 1 cut(s) 165
DpnI GATC 6 cut(s) 336, 438, 453, 539, 792, 1200
DpnII GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
EaeI YGGCCR 2 cut(s) 586, 1290
Ecl136II GAGCTC 2 cut(s) 144, 711
Eco130I CCWWGG 4 cut(s) 237, 992, 1090, 1293
Eco24I GRGCYC 3 cut(s) 146, 507, 713
Eco53kI GAGCTC 2 cut(s) 144, 711
Eco81I CCTNAGG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 145
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 2 cut(s) 144, 711
EcoO109I RGGNCCY 1 cut(s) 1263
EcoO65I GGTNACC 1 cut(s) 1135
EcoRII CCWGG 4 cut(s) 167, 495, 923, 1259
EcoT14I CCWWGG 4 cut(s) 237, 992, 1090, 1293
EcoT22I ATGCAT 1 cut(s) 626
EcoT38I GRGCYC 3 cut(s) 146, 507, 713
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 4 cut(s) 237, 992, 1090, 1293
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 2 cut(s) 941, 987
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 4 cut(s) 72, 234, 1107, 1336
FokI GGATG 4 cut(s) 479, 512, 741, 1344
FriOI GRGCYC 3 cut(s) 146, 507, 713
Fsp4HI GCNGC 4 cut(s) 72, 234, 1107, 1336
FspBI CTAG 1 cut(s) 1354
GlaI GCGC 2 cut(s) 165, 482
GluI GCNGC 4 cut(s) 72, 234, 1107, 1336
GsaI CCCAGC 1 cut(s) 284
GsuI CTGGAG 3 cut(s) 1204, 1254, 1260
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 5 cut(s) 35, 588, 906, 1265, 1292
HapII CCGG 2 cut(s) 81, 471
HgaI GACGC 3 cut(s) 350, 426, 1233
HhaI GCGC 2 cut(s) 166, 483
Hin1I GRCGYC 1 cut(s) 164
Hin6I GCGC 2 cut(s) 164, 481
HinP1I GCGC 2 cut(s) 164, 481
HincII GTYRAC 3 cut(s) 244, 460, 846
HindII GTYRAC 3 cut(s) 244, 460, 846
HindIII AAGCTT 1 cut(s) 1270
HinfI GANTC 2 cut(s) 594, 914
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 2 cut(s) 81, 471
HphI GGTGA 6 cut(s) 184, 547, 715, 761, 1033, 1208
Hpy166II GTNNAC 6 cut(s) 244, 460, 604, 846, 1024, 1325
Hpy188I TCNGA 2 cut(s) 13, 1192
Hpy188III TCNNGA 7 cut(s) 115, 410, 511, 562, 574, 982, 1233
Hpy8I GTNNAC 6 cut(s) 244, 460, 604, 846, 1024, 1325
Hpy99I CGWCG 2 cut(s) 71, 101
HpyAV CCTTC 2 cut(s) 1187, 1228
HpyCH4III ACNGT 2 cut(s) 101, 374
HpyCH4IV ACGT 2 cut(s) 526, 1345
HpyF10VI GCNNNNNNNGC 3 cut(s) 480, 617, 1256
HpyF3I CTNAG 2 cut(s) 113, 1164
HpySE526I ACGT 2 cut(s) 526, 1345
Hsp92I GRCGYC 1 cut(s) 164
HspAI GCGC 2 cut(s) 164, 481
KasI GGCGCC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 790
KspAI GTTAAC 1 cut(s) 460
Kzo9I GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
LmnI GCTCC 8 cut(s) 553, 617, 708, 716, 1114, 1235, 1241, 1277
Lsp1109I GCAGC 3 cut(s) 58, 220, 1118
LweI GCATC 3 cut(s) 742, 820, 1322
MaeI CTAG 1 cut(s) 1354
MaeII ACGT 2 cut(s) 526, 1345
MaeIII GTNAC 5 cut(s) 430, 527, 640, 943, 1135
MalI GATC 6 cut(s) 336, 438, 453, 539, 792, 1200
MboI GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
MboII GAAGA 4 cut(s) 44, 757, 1193, 1213
MfeI CAATTG 2 cut(s) 542, 808
MhlI GDGCHC 4 cut(s) 146, 507, 713, 1327
MluCI AATT 6 cut(s) 151, 228, 378, 542, 796, 808
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 1 cut(s) 603
MmeI TCCRAC 2 cut(s) 888, 1136
Mph1103I ATGCAT 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 569
MseI TTAA 1 cut(s) 459
MslI CAYNNNNRTG 3 cut(s) 672, 885, 1330
MspA1I CMGCKG 1 cut(s) 1250
MspI CCGG 2 cut(s) 81, 471
MspR9I CCNGG 5 cut(s) 169, 471, 497, 925, 1261
MunI CAATTG 2 cut(s) 542, 808
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 4 cut(s) 169, 497, 925, 1261
MwoI GCNNNNNNNGC 3 cut(s) 480, 617, 1256
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 1 cut(s) 471
NcoI CCATGG 2 cut(s) 237, 1293
NdeII GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
NlaIV GGNNCC 5 cut(s) 103, 165, 1216, 1279, 1303
NmuCI GTSAC 4 cut(s) 430, 527, 640, 943
NsiI ATGCAT 1 cut(s) 626
NspI RCATGY 2 cut(s) 209, 624
PaeI GCATGC 2 cut(s) 209, 624
PaeR7I CTCGAG 1 cut(s) 145
PagI TCATGA 1 cut(s) 561
PasI CCCWGGG 1 cut(s) 168
PctI GAATGC 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 1 cut(s) 914
PfoI TCCNGGA 1 cut(s) 495
PkrI GCNGC 4 cut(s) 73, 235, 1108, 1337
PleI GAGTC 1 cut(s) 602
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 602
Psp124BI GAGCTC 2 cut(s) 146, 713
Psp6I CCWGG 4 cut(s) 167, 495, 923, 1259
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 4 cut(s) 167, 495, 923, 1259
PspN4I GGNNCC 5 cut(s) 103, 165, 1216, 1279, 1303
PspPI GGNCC 2 cut(s) 904, 1263
PspXI VCTCGAGB 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 1016, 1048
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 2 cut(s) 103, 1348
RsaNI GTAC 2 cut(s) 102, 1347
RseI CAYNNNNRTG 3 cut(s) 672, 885, 1330
SacI GAGCTC 2 cut(s) 146, 713
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 4 cut(s) 72, 234, 1107, 1336
Sau3AI GATC 6 cut(s) 334, 436, 451, 537, 790, 1198
Sau96I GGNCC 2 cut(s) 904, 1263
SchI GAGTC 1 cut(s) 603
ScrFI CCNGG 5 cut(s) 169, 471, 497, 925, 1261
SduI GDGCHC 4 cut(s) 146, 507, 713, 1327
SfaNI GCATC 3 cut(s) 742, 820, 1322
SfoI GGCGCC 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 145
SlaI CTCGAG 1 cut(s) 145
SmiMI CAYNNNNRTG 3 cut(s) 672, 885, 1330
SmlI CTYRAG 2 cut(s) 145, 1267
SmoI CTYRAG 2 cut(s) 145, 1267
SphI GCATGC 2 cut(s) 209, 624
Sse9I AATT 6 cut(s) 151, 228, 378, 542, 796, 808
SsiI CCGC 4 cut(s) 5, 256, 355, 1336
SspDI GGCGCC 1 cut(s) 163
SspMI CTAG 1 cut(s) 1354
SstI GAGCTC 2 cut(s) 146, 713
StyD4I CCNGG 5 cut(s) 167, 469, 495, 923, 1259
StyI CCWWGG 4 cut(s) 237, 992, 1090, 1293
TaaI ACNGT 2 cut(s) 101, 374
TaiI ACGT 2 cut(s) 529, 1348
TaqI TCGA 2 cut(s) 96, 146
TasI AATT 6 cut(s) 151, 228, 378, 542, 796, 808
TauI GCSGC 1 cut(s) 1338
TfiI GAWTC 1 cut(s) 914
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 4 cut(s) 271, 439, 1046, 1172
TseFI GTSAC 4 cut(s) 430, 527, 640, 943
TseI GCWGC 3 cut(s) 71, 233, 1106
Tsp45I GTSAC 4 cut(s) 430, 527, 640, 943
TspDTI ATGAA 5 cut(s) 144, 240, 578, 647, 903
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 4 cut(s) 271, 439, 1046, 1172
VneI GTGCAC 1 cut(s) 1323
XceI RCATGY 2 cut(s) 209, 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XhoI CTCGAG 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 569
XspI CTAG 1 cut(s) 1354
Zsp2I ATGCAT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.