MD13G1231000.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010648
Physical Location & Seq
Reverse (-)
22877590 .. 22880954
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1231000.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGCGGTGTTTGAGAGTAAGAAGAAATGGGTCGGTGCGTTCGTGACGGTGCTTCTCGTGTGTTGCTTCGCAGCTGCTGTGGTCTCCGAGATCTCTCGCAACAGCAATGGAGGAACAGAGGAGTTGCAGAGCTCGAGCAACTCGTCAATGGCGGCCAGAGTGGCTGAGGATGATGAAGGATTCAACAAGCATGCAGTTGAAGACCCAGAAGAGATTGCTTCTATGGTTGACATGAGTATCCGCAACAGCACGGAGAGGAGGAAGCTGGGCTACTTCTCGTGTGGAACTGGCAATCCAATCGATGACTGCTGGCGCTGTGATCCCAACTGGCACAAAAACCGCAAGCGTCTTGCAGACTGTGGCATTGGTTTTGGGAGAAATGCCATTGGTGGCCGCGATGGACGCTTCTATGTTGTCACCGACCCTAATGATGATGATCCTGTTAACCCCAGGCCTGGTACTCTTCGCCATGCTGTCATCCAGACCAAGCCTCTCTGGATTGTGTTCAAGCGAGACATGGTGATACGGTTGAAGCAGGAGCTCATCATGAACAGCTTCAAGACCATTGATGGTCGCGGAGTCAATGTTCACATTGCTAATGGAGGATGCATCACAATCCAATTTGTTACAAATATTATCATCCACGGCCTGCACATCCATGACTGCAAGCCCACTGGCAATGCTTTGGTGAGGAGCTCGCCTACTCACTTTGGGTGGCGGACAATGGCTGATGGAGATGCTGTCTCCATCTTCGGGTCAAGCCATATTTGGGTTGATCACAATTCCCTCTCCAACTGCGCTGATGGTCTCGTTGATGCTGTCATGGGCTCAACTGCAATTACCATTTCCAACAACCACATGACCCACCACAATGAGGTGATGCTGCTGGGCCACAGTGATAGCTATACCAGAGACAAGCAAATGCAAGTCACAATTGCTTACAACCACTTTGGAGAGGGACTTATCCAAAGAATGCCAAGGTGCAGGCACGGGTATTTCCATGTCGTGAACAATGACTACACTCACTGGGAAATGTATGCCATTGGTGGGAGTGCAGAACCCACTATCAACAGCCAAGGCAACAGATATGCTGCTCCAACCAACCCCTTTGCAAAGGAGGTTACCAAGAGGGTCGAGACGGCTGCCACTAAATGGAAGAACTGGAACTGGAGATCAGAAGGAGACCTGCTGCTCAATGGTGCCTACTTCACTCCATCTGGAGCTGGAGCTTCAGCAAGCTACGCCAGGGCCTCAAGCTTGGGAGCCAAGTCATCTGCCATGGTTGGAGCCATAACTTCAGGTTCCGGTGCACTTCCCTGCCGCAGAGGCCACCCCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

447

Amino Acids

48.92

Weight (kDa)

8.22

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 180 - 380 1.7e-19 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24230 AT4G13710 AT4G13710
fragaria_vesca FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760
malus_domestica MD13G1231000.v1.1 MD16G1236000.v1.1
prunus_persica Prupe.1G060900_v2.0.a1
pyrus_communis pycom13g20360 pycom16g19790
rosa_chinensis RchiOBHm_Chr4g0396921
rosa_laevigata RLG00000009489
rosa_roxburghii Rroxscaffold_5G00341840
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075300 Rh4DG069100
rosa_wichuraiana Rw4G006040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1194
AccB1I GGYRCC 1 cut(s) 1199
AccB7I CCANNNNNTGG 1 cut(s) 1152
AccII CGCG 2 cut(s) 396, 576
AciI CCGC 8 cut(s) 5, 152, 241, 340, 394, 576, 718, 1321
AclWI GGATC 2 cut(s) 314, 431
AcoI YGGCCR 2 cut(s) 153, 391
AcuI CTGAAG 2 cut(s) 1215, 1281
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 6 cut(s) 455, 753, 769, 874, 1047, 1152
AgsI TTSAA 5 cut(s) 184, 200, 508, 532, 559
AjnI CCWGG 3 cut(s) 449, 454, 1244
AloI GAACNNNNNNTCC 4 cut(s) 277, 309, 570, 602
Alw21I GWGCWC 4 cut(s) 134, 543, 698, 1312
Alw26I GTCTC 7 cut(s) 88, 507, 748, 812, 906, 1130, 1176
Alw44I GTGCAC 1 cut(s) 1308
AlwI GGATC 2 cut(s) 314, 431
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 7 cut(s) 153, 391, 452, 646, 889, 1248, 1327
ApaLI GTGCAC 1 cut(s) 1308
ApeKI GCWGC 6 cut(s) 71, 74, 883, 1091, 1142, 1189
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 2 cut(s) 315, 800
AspS9I GGNCC 2 cut(s) 889, 1248
AsuHPI GGTGA 4 cut(s) 409, 532, 700, 889
AvaI CYCGRG 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1312
BaeI ACNNNNGTAYC 2 cut(s) 220, 253
BanI GGYRCC 1 cut(s) 1199
BanII GRGCYC 4 cut(s) 134, 543, 698, 830
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BauI CACGAG 2 cut(s) 56, 277
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 4 cut(s) 134, 543, 698, 1312
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 6 cut(s) 61, 83, 870, 1078, 1129, 1176
BccI CCATC 6 cut(s) 392, 563, 725, 755, 797, 1222
BceAI ACGGC 2 cut(s) 661, 1155
BcgI CGANNNNNNTGC 4 cut(s) 280, 314, 1124, 1158
BciT130I CCWGG 3 cut(s) 451, 456, 1246
BciVI GTATCC 1 cut(s) 248
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 7 cut(s) 88, 507, 748, 812, 906, 1130, 1176
BfaI CTAG 1 cut(s) 1339
BfoI RGCGCY 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 1194
BfuI GTATCC 1 cut(s) 248
BglI GCCNNNNNGGC 2 cut(s) 161, 1326
BglII AGATCT 1 cut(s) 90
BisI GCNGC 9 cut(s) 72, 75, 153, 394, 884, 1092, 1143, 1190, 1321
BlsI GCNGC 9 cut(s) 73, 76, 154, 395, 885, 1093, 1144, 1191, 1322
Bme1390I CCNGG 3 cut(s) 451, 456, 1246
BmeT110I CYCGRG 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 889, 1248
BmiI GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
BmrFI CCNGG 3 cut(s) 451, 456, 1246
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 5 cut(s) 596, 618, 727, 805, 870
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 207
BpmI CTGGAG 3 cut(s) 1189, 1239, 1245
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 1237
Bsa29I ATCGAT 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 435
BsaI GGTCTC 3 cut(s) 88, 812, 1176
BsaJI CCNNGG 6 cut(s) 449, 643, 977, 1075, 1245, 1278
BsaWI WCCGGW 1 cut(s) 1304
BsaXI ACNNNNNCTCC 4 cut(s) 570, 600, 726, 756
Bsc4I CCNNNNNNNGG 6 cut(s) 455, 753, 769, 874, 1047, 1152
Bse1I ACTGG 6 cut(s) 292, 332, 679, 1031, 1166, 1172
Bse3DI GCAATG 3 cut(s) 112, 591, 685
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 3 cut(s) 451, 456, 1246
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 6 cut(s) 449, 643, 977, 1075, 1245, 1278
BseGI GGATG 5 cut(s) 175, 477, 611, 639, 654
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 6 cut(s) 455, 753, 769, 874, 1047, 1152
BseMI GCAATG 3 cut(s) 112, 591, 685
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 6 cut(s) 292, 332, 679, 1031, 1166, 1172
BseRI GAGGAG 3 cut(s) 134, 271, 706
BseSI GKGCMC 1 cut(s) 1312
BseXI GCAGC 6 cut(s) 61, 83, 870, 1078, 1129, 1176
BseYI CCCAGC 2 cut(s) 265, 886
BsgI GTGCAG 3 cut(s) 635, 1003, 1074
Bsh1236I CGCG 2 cut(s) 396, 576
BshFI GGCC 7 cut(s) 155, 393, 454, 648, 891, 1250, 1329
BshNI GGYRCC 1 cut(s) 1199
BshVI ATCGAT 1 cut(s) 300
BsiHKAI GWGCWC 4 cut(s) 134, 543, 698, 1312
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 1 cut(s) 1305
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 6 cut(s) 455, 753, 769, 874, 1047, 1152
BsmAI GTCTC 7 cut(s) 88, 507, 748, 812, 906, 1130, 1176
BsmBI CGTCTC 1 cut(s) 1130
BsmFI GGGAC 1 cut(s) 972
BsmI GAATGC 1 cut(s) 978
BsnI GGCC 7 cut(s) 155, 393, 454, 648, 891, 1250, 1329
Bso31I GGTCTC 3 cut(s) 88, 812, 1176
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
Bsp143I GATC 5 cut(s) 90, 319, 436, 775, 1172
Bsp19I CCATGG 1 cut(s) 1278
BspACI CCGC 8 cut(s) 5, 152, 241, 340, 394, 576, 718, 1321
BspANI GGCC 7 cut(s) 155, 393, 454, 648, 891, 1250, 1329
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 2 cut(s) 396, 576
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
BspMI ACCTGC 1 cut(s) 1194
BspPI GGATC 2 cut(s) 314, 431
BspT107I GGYRCC 1 cut(s) 1199
BspTNI GGTCTC 3 cut(s) 88, 812, 1176
BsrDI GCAATG 3 cut(s) 112, 591, 685
BsrI ACTGG 6 cut(s) 292, 332, 679, 1031, 1166, 1172
BssECI CCNNGG 6 cut(s) 449, 643, 977, 1075, 1245, 1278
BssMI GATC 5 cut(s) 90, 319, 436, 775, 1172
BssSI CACGAG 2 cut(s) 56, 277
BssT1I CCWWGG 3 cut(s) 977, 1075, 1278
Bst2BI CACGAG 2 cut(s) 56, 277
Bst2UI CCWGG 3 cut(s) 451, 456, 1246
Bst4CI ACNGT 4 cut(s) 49, 359, 528, 896
Bst6I CTCTTC 2 cut(s) 204, 468
BstC8I GCNNGC 8 cut(s) 192, 311, 344, 650, 668, 698, 986, 1237
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 2 cut(s) 643, 1278
BstEII GGTNACC 1 cut(s) 1120
BstF5I GGATG 5 cut(s) 175, 477, 611, 639, 654
BstFNI CGCG 2 cut(s) 396, 576
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 2 cut(s) 315, 800
BstKTI GATC 5 cut(s) 93, 322, 439, 778, 1175
BstMAI GTCTC 7 cut(s) 88, 507, 748, 812, 906, 1130, 1176
BstMBI GATC 5 cut(s) 90, 319, 436, 775, 1172
BstMWI GCNNNNNNNGC 5 cut(s) 161, 402, 1241, 1326, 1335
BstNI CCWGG 3 cut(s) 451, 456, 1246
BstNSI RCATGY 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 1120
BstSCI CCNGG 3 cut(s) 449, 454, 1244
BstSLI GKGCMC 1 cut(s) 1312
BstUI CGCG 2 cut(s) 396, 576
BstV1I GCAGC 6 cut(s) 61, 83, 870, 1078, 1129, 1176
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
Bsu15I ATCGAT 1 cut(s) 300
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 7 cut(s) 155, 393, 454, 648, 891, 1250, 1329
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 2 cut(s) 643, 1278
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 5 cut(s) 175, 477, 611, 639, 654
BtsIMutI CAGTG 3 cut(s) 672, 901, 1024
BveI ACCTGC 1 cut(s) 1194
Cac8I GCNNGC 8 cut(s) 192, 311, 344, 650, 668, 698, 986, 1237
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 1 cut(s) 546
CfoI GCGC 2 cut(s) 315, 800
Cfr13I GGNCC 2 cut(s) 889, 1248
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 2 cut(s) 335, 411
Csp6I GTAC 1 cut(s) 459
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 165
DpnI GATC 5 cut(s) 92, 321, 438, 777, 1174
DpnII GATC 5 cut(s) 90, 319, 436, 775, 1172
EaeI YGGCCR 2 cut(s) 153, 391
Eam1104I CTCTTC 2 cut(s) 204, 468
EarI CTCTTC 2 cut(s) 204, 468
EciI GGCGGA 1 cut(s) 733
Ecl136II GAGCTC 3 cut(s) 132, 541, 696
Eco130I CCWWGG 3 cut(s) 977, 1075, 1278
Eco147I AGGCCT 1 cut(s) 454
Eco24I GRGCYC 4 cut(s) 134, 543, 698, 830
Eco31I GGTCTC 3 cut(s) 88, 812, 1176
Eco53kI GAGCTC 3 cut(s) 132, 541, 696
Eco57I CTGAAG 2 cut(s) 1215, 1281
Eco88I CYCGRG 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 1120
EcoICRI GAGCTC 3 cut(s) 132, 541, 696
EcoO109I RGGNCCY 1 cut(s) 1248
EcoO65I GGTNACC 1 cut(s) 1120
EcoRII CCWGG 3 cut(s) 449, 454, 1244
EcoT14I CCWWGG 3 cut(s) 977, 1075, 1278
EcoT22I ATGCAT 1 cut(s) 611
EcoT38I GRGCYC 4 cut(s) 134, 543, 698, 830
ErhI CCWWGG 3 cut(s) 977, 1075, 1278
Esp3I CGTCTC 1 cut(s) 1130
FaqI GGGAC 1 cut(s) 972
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 9 cut(s) 72, 75, 153, 394, 884, 1092, 1143, 1190, 1321
FokI GGATG 5 cut(s) 182, 464, 618, 626, 641
FriOI GRGCYC 4 cut(s) 134, 543, 698, 830
Fsp4HI GCNGC 9 cut(s) 72, 75, 153, 394, 884, 1092, 1143, 1190, 1321
FspBI CTAG 1 cut(s) 1339
GlaI GCGC 2 cut(s) 314, 799
GluI GCNGC 9 cut(s) 72, 75, 153, 394, 884, 1092, 1143, 1190, 1321
GsaI CCCAGC 2 cut(s) 269, 890
GsuI CTGGAG 3 cut(s) 1189, 1239, 1245
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 7 cut(s) 155, 393, 454, 648, 891, 1250, 1329
HapII CCGG 1 cut(s) 1305
HgaI GACGC 2 cut(s) 335, 411
HhaI GCGC 2 cut(s) 315, 800
Hin6I GCGC 2 cut(s) 313, 798
HinP1I GCGC 2 cut(s) 313, 798
HincII GTYRAC 2 cut(s) 229, 445
HindII GTYRAC 2 cut(s) 229, 445
HindIII AAGCTT 1 cut(s) 1255
HinfI GANTC 2 cut(s) 180, 579
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 1 cut(s) 1305
HphI GGTGA 4 cut(s) 409, 532, 700, 889
Hpy166II GTNNAC 5 cut(s) 229, 445, 589, 1009, 1310
Hpy188I TCNGA 2 cut(s) 88, 1177
Hpy188III TCNNGA 8 cut(s) 43, 481, 496, 547, 559, 1006, 1135, 1218
Hpy8I GTNNAC 5 cut(s) 229, 445, 589, 1009, 1310
HpyAV CCTTC 2 cut(s) 170, 1172
HpyCH4III ACNGT 4 cut(s) 49, 359, 528, 896
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 402, 1241, 1326, 1335
HpyF3I CTNAG 1 cut(s) 165
HspAI GCGC 2 cut(s) 313, 798
Ksp22I TGATCA 1 cut(s) 775
KspAI GTTAAC 1 cut(s) 445
Kzo9I GATC 5 cut(s) 90, 319, 436, 775, 1172
LmnI GCTCC 7 cut(s) 538, 693, 1099, 1220, 1226, 1262, 1286
Lsp1109I GCAGC 6 cut(s) 61, 83, 870, 1078, 1129, 1176
LweI GCATC 5 cut(s) 596, 618, 727, 805, 870
MaeI CTAG 1 cut(s) 1339
MaeIII GTNAC 5 cut(s) 43, 415, 625, 928, 1120
MalI GATC 5 cut(s) 92, 321, 438, 777, 1174
MboI GATC 5 cut(s) 90, 319, 436, 775, 1172
MboII GAAGA 6 cut(s) 34, 212, 221, 455, 742, 1168
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
MluCI AATT 4 cut(s) 620, 781, 837, 933
MlyI GAGTC 1 cut(s) 588
MmeI TCCRAC 4 cut(s) 816, 873, 1121, 1264
Mph1103I ATGCAT 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 2 cut(s) 657, 870
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 1305
MspR9I CCNGG 3 cut(s) 451, 456, 1246
MunI CAATTG 1 cut(s) 933
Mva1269I GAATGC 1 cut(s) 978
MvaI CCWGG 3 cut(s) 451, 456, 1246
MvnI CGCG 2 cut(s) 396, 576
MwoI GCNNNNNNNGC 5 cut(s) 161, 402, 1241, 1326, 1335
NcoI CCATGG 1 cut(s) 1278
NdeII GATC 5 cut(s) 90, 319, 436, 775, 1172
NlaIV GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
NmuCI GTSAC 3 cut(s) 43, 415, 928
NsiI ATGCAT 1 cut(s) 611
NspI RCATGY 1 cut(s) 194
PaeI GCATGC 1 cut(s) 194
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 1 cut(s) 546
PceI AGGCCT 1 cut(s) 454
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 140
PctI GAATGC 1 cut(s) 978
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 180
PflMI CCANNNNNTGG 1 cut(s) 1152
PkrI GCNGC 9 cut(s) 73, 76, 154, 395, 885, 1093, 1144, 1191, 1322
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp124BI GAGCTC 3 cut(s) 134, 543, 698
Psp6I CCWGG 3 cut(s) 449, 454, 1244
PspEI GGTNACC 1 cut(s) 1120
PspFI CCCAGC 2 cut(s) 265, 886
PspGI CCWGG 3 cut(s) 449, 454, 1244
PspN4I GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
PspPI GGNCC 2 cut(s) 889, 1248
PspXI VCTCGAGB 1 cut(s) 133
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
RseI CAYNNNNRTG 2 cut(s) 657, 870
SacI GAGCTC 3 cut(s) 134, 543, 698
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 9 cut(s) 72, 75, 153, 394, 884, 1092, 1143, 1190, 1321
Sau3AI GATC 5 cut(s) 90, 319, 436, 775, 1172
Sau96I GGNCC 2 cut(s) 889, 1248
SchI GAGTC 1 cut(s) 588
ScrFI CCNGG 3 cut(s) 451, 456, 1246
SduI GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
SfaNI GCATC 5 cut(s) 596, 618, 727, 805, 870
Sfr274I CTCGAG 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 657, 870
SmlI CTYRAG 2 cut(s) 133, 1252
SmoI CTYRAG 2 cut(s) 133, 1252
SphI GCATGC 1 cut(s) 194
Sse9I AATT 4 cut(s) 620, 781, 837, 933
SseBI AGGCCT 1 cut(s) 454
SsiI CCGC 8 cut(s) 5, 152, 241, 340, 394, 576, 718, 1321
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 1339
SstI GAGCTC 3 cut(s) 134, 543, 698
StuI AGGCCT 1 cut(s) 454
StyD4I CCNGG 3 cut(s) 449, 454, 1244
StyI CCWWGG 3 cut(s) 977, 1075, 1278
TaaI ACNGT 4 cut(s) 49, 359, 528, 896
TaqI TCGA 3 cut(s) 134, 300, 1134
TasI AATT 4 cut(s) 620, 781, 837, 933
TauI GCSGC 3 cut(s) 155, 396, 1323
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 3 cut(s) 679, 901, 1031
TseFI GTSAC 3 cut(s) 43, 415, 928
TseI GCWGC 6 cut(s) 71, 74, 883, 1091, 1142, 1189
Tsp45I GTSAC 3 cut(s) 43, 415, 928
TspDTI ATGAA 2 cut(s) 189, 563
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 3 cut(s) 679, 901, 1031
Van91I CCANNNNNTGG 1 cut(s) 1152
VneI GTGCAC 1 cut(s) 1308
XceI RCATGY 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 1339
Zsp2I ATGCAT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.