Prupe.1G060900_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010648
Physical Location & Seq
Reverse (-)
4287815 .. 4292890
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G060900.2

Sequence Viewer

Length: 1338 bp
ATGGCGGTGTTTGAGAGTAAGAGATGGGTTACTGGTGCTTTCTTGGCGGTGCTTCTCGTGCTTTGCTTCGTTGCTGCAATTGCTGAGATCTCTGGAAACAGAAATGGAGGAACAGAGGAGTTGCAGAGCTCAAGCAACTCATCAATGGCGGCCAGGGTGGCTGAGGATGATGAGAGCTTCAATAAGCATGCAGTTGATGACCCAGAGGAGGTTGTTGCCATGGTTGACATGAGTATCCGAAACAGCACTGAGAGAAGGAAGCTGGGCTTCTTCTCATGTGGAACTGGCAATCCAATTGATGACTGCTGGCGTTGTGATTCAAACTGGCAGAAAAACCGCAAGCGTCTTGCAGACTGCGGCATTGGTTTTGGCAGAAATGCCATTGGTGGTCGTGATGGTCGCTTCTATGTTGTCACTGACCCTGGTGATGATGATCCTGTTAACCCCAGACCCGGTACTCTTCGCCACGCTGTCATCCAGAACGAGCCTCTCTGGATTGTGTTCAAGCGAGACATGGTGATACAATTGAAGCAGGAGCTCATCATGAACAGCTTCAAGACCATTGATGGCCGCGGAGTCAATGTTCACATTGCTAATGGAGCATGCATCACAATCCAATTTGTTACAAATATCATAATTCATGGTCTACATATCCATGACTGCAAGCCCACAGGAAATGCTTTGGTGAGGAGCTCTCCATCTCATTTCGGGTGGCGGACAATGGCTGATGGTGATGCTGTCTCCATCTTCGGGTCCAGCCATATATGGGTTGACCACAATTCTCTCTCCAACTGTGCTGATGGTCTTGTTGATGCTGTCATGGGCTCAACTGCAATTACCATTTCCAACAACCACTTTACCCACCACAATGAGGTGATGCTGCTGGGCCACAGTGACTCTTATACCAGAGACAAGGCAATGCAAGTGACAATTGCTTACAACCACTTTGGGGAGGGACTTATCCAAAGAATGCCAAGGTGTAGGCACGGGTATTTCCATGTGGTGAACAACGACTACACTCACTGGGAAATGTATGCCATTGGTGGAAGTGCAGAGCCCACCATCAACAGCCAGGGCAACAGATATGCTGCTCCAACCAACCCCTTTGCAAAGGAGGTTACCAAGAGGGTCGAGACACCGACAACCCAATGGAAGAGCTGGAACTGGAGATCAGAAGGAGACCTGCTTCTGAATGGTGCCTACTTCACTCCATCCGGAGCTGGAGCTTCAGCAAGCTATGCCAGGGCCTCAAGCTTGGGAGCCAAGTCCTCTGCCATGGTTGGAGCCATAACTTCAGGTTCTGGTGCACTCCCCTGCCGCAGAGGCCATCCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

446

Amino Acids

48.71

Weight (kDa)

6.8

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24230 AT4G13710 AT4G13710
fragaria_vesca FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760
malus_domestica MD13G1231000.v1.1 MD16G1236000.v1.1
prunus_persica Prupe.1G060900_v2.0.a1
pyrus_communis pycom13g20360 pycom16g19790
rosa_chinensis RchiOBHm_Chr4g0396921
rosa_laevigata RLG00000009489
rosa_roxburghii Rroxscaffold_5G00341840
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075300 Rh4DG069100
rosa_wichuraiana Rw4G006040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1191
AccB1I GGYRCC 1 cut(s) 1196
AccI GTMKAC 1 cut(s) 646
AccII CGCG 1 cut(s) 573
AccIII TCCGGA 1 cut(s) 1214
AciI CCGC 9 cut(s) 5, 47, 149, 337, 357, 571, 573, 715, 1318
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 2 cut(s) 150, 568
AcuI CTGAAG 2 cut(s) 1212, 1278
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 6 cut(s) 208, 452, 750, 766, 871, 949
AgsI TTSAA 5 cut(s) 181, 321, 505, 529, 556
AjnI CCWGG 4 cut(s) 152, 421, 1071, 1241
AloI GAACNNNNNNTCC 4 cut(s) 274, 306, 567, 599
Alw21I GWGCWC 4 cut(s) 131, 540, 695, 1309
Alw26I GTCTC 5 cut(s) 504, 745, 903, 1127, 1173
Alw44I GTGCAC 1 cut(s) 1305
AlwI GGATC 1 cut(s) 428
AlwNI CAGNNNCTG 1 cut(s) 1301
Aor13HI TCCGGA 1 cut(s) 1214
AoxI GGCC 5 cut(s) 150, 568, 886, 1245, 1324
ApaLI GTGCAC 1 cut(s) 1305
ApeKI GCWGC 3 cut(s) 74, 880, 1088
Asp700I GAANNNNTTC 1 cut(s) 551
AspS9I GGNCC 3 cut(s) 753, 886, 1245
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 6 cut(s) 437, 529, 697, 743, 886, 1015
AvaII GGWCC 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 1309
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 5 cut(s) 131, 540, 695, 827, 1059
BauI CACGAG 1 cut(s) 56
Bbv12I GWGCWC 4 cut(s) 131, 540, 695, 1309
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 61, 867, 1075
BciT130I CCWGG 4 cut(s) 154, 423, 1073, 1243
BciVI GTATCC 1 cut(s) 245
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 5 cut(s) 504, 745, 903, 1127, 1173
BfaI CTAG 1 cut(s) 1336
BfuAI ACCTGC 1 cut(s) 1191
BfuI GTATCC 1 cut(s) 245
BglI GCCNNNNNGGC 2 cut(s) 158, 1323
BglII AGATCT 1 cut(s) 87
BisI GCNGC 7 cut(s) 75, 150, 358, 571, 881, 1089, 1318
BlsI GCNGC 7 cut(s) 76, 151, 359, 572, 882, 1090, 1319
Bme1390I CCNGG 5 cut(s) 154, 423, 453, 1073, 1243
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 3 cut(s) 753, 886, 1245
BmiI GGNNCC 4 cut(s) 754, 1198, 1261, 1285
BmrFI CCNGG 5 cut(s) 154, 423, 453, 1073, 1243
BmrI ACTGGG 1 cut(s) 1033
BmsI GCATC 4 cut(s) 615, 724, 802, 867
BmuI ACTGGG 1 cut(s) 1033
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpmI CTGGAG 2 cut(s) 1186, 1242
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 115, 1234
BpuMI CCSGG 1 cut(s) 453
BsaBI GATNNNNATC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 1173
BsaJI CCNNGG 8 cut(s) 153, 219, 421, 571, 974, 1072, 1242, 1275
BsaWI WCCGGW 1 cut(s) 1214
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 6 cut(s) 208, 452, 750, 766, 871, 949
Bse1I ACTGG 5 cut(s) 37, 289, 329, 1028, 1169
Bse3DI GCAATG 2 cut(s) 588, 924
Bse8I GATNNNNATC 1 cut(s) 432
BseAI TCCGGA 1 cut(s) 1214
BseBI CCWGG 4 cut(s) 154, 423, 1073, 1243
BseDI CCNNGG 8 cut(s) 153, 219, 421, 571, 974, 1072, 1242, 1275
BseGI GGATG 4 cut(s) 172, 474, 1211, 1327
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 6 cut(s) 208, 452, 750, 766, 871, 949
BseMI GCAATG 2 cut(s) 588, 924
BseMII CTCAG 3 cut(s) 75, 153, 240
BseNI ACTGG 5 cut(s) 37, 289, 329, 1028, 1169
BseRI GAGGAG 3 cut(s) 131, 221, 703
BseSI GKGCMC 1 cut(s) 1309
BseXI GCAGC 3 cut(s) 61, 867, 1075
BseYI CCCAGC 2 cut(s) 262, 883
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 1 cut(s) 573
BshFI GGCC 5 cut(s) 152, 570, 888, 1247, 1326
BshNI GGYRCC 1 cut(s) 1196
BsiHKAI GWGCWC 4 cut(s) 131, 540, 695, 1309
BsiSI CCGG 2 cut(s) 453, 1215
BslFI GGGAC 1 cut(s) 969
BslI CCNNNNNNNGG 6 cut(s) 208, 452, 750, 766, 871, 949
BsmAI GTCTC 5 cut(s) 504, 745, 903, 1127, 1173
BsmFI GGGAC 1 cut(s) 969
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 5 cut(s) 152, 570, 888, 1247, 1326
Bso31I GGTCTC 1 cut(s) 1173
Bsp1286I GDGCHC 6 cut(s) 131, 540, 695, 827, 1059, 1309
Bsp13I TCCGGA 1 cut(s) 1214
Bsp143I GATC 3 cut(s) 87, 433, 1169
Bsp19I CCATGG 2 cut(s) 219, 1275
BspACI CCGC 9 cut(s) 5, 47, 149, 337, 357, 571, 573, 715, 1318
BspANI GGCC 5 cut(s) 152, 570, 888, 1247, 1326
BspCNI CTCAG 3 cut(s) 76, 154, 241
BspEI TCCGGA 1 cut(s) 1214
BspFNI CGCG 1 cut(s) 573
BspHI TCATGA 1 cut(s) 543
BspLI GGNNCC 4 cut(s) 754, 1198, 1261, 1285
BspMI ACCTGC 1 cut(s) 1191
BspPI GGATC 1 cut(s) 428
BspQI GCTCTTC 1 cut(s) 1148
BspT107I GGYRCC 1 cut(s) 1196
BspTNI GGTCTC 1 cut(s) 1173
BsrDI GCAATG 2 cut(s) 588, 924
BsrI ACTGG 5 cut(s) 37, 289, 329, 1028, 1169
BssECI CCNNGG 8 cut(s) 153, 219, 421, 571, 974, 1072, 1242, 1275
BssMI GATC 3 cut(s) 87, 433, 1169
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 3 cut(s) 219, 974, 1275
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 4 cut(s) 154, 423, 1073, 1243
Bst4CI ACNGT 2 cut(s) 794, 893
Bst6I CTCTTC 2 cut(s) 465, 1148
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstC8I GCNNGC 6 cut(s) 189, 308, 341, 604, 665, 1234
BstDEI CTNAG 3 cut(s) 84, 162, 249
BstDSI CCRYGG 3 cut(s) 219, 571, 1275
BstEII GGTNACC 1 cut(s) 1117
BstF5I GGATG 4 cut(s) 172, 474, 1211, 1327
BstFNI CGCG 1 cut(s) 573
BstKTI GATC 3 cut(s) 90, 436, 1172
BstMAI GTCTC 5 cut(s) 504, 745, 903, 1127, 1173
BstMBI GATC 3 cut(s) 87, 433, 1169
BstMWI GCNNNNNNNGC 8 cut(s) 44, 58, 80, 158, 599, 1238, 1323, 1332
BstNI CCWGG 4 cut(s) 154, 423, 1073, 1243
BstNSI RCATGY 2 cut(s) 191, 606
BstPI GGTNACC 1 cut(s) 1117
BstSCI CCNGG 5 cut(s) 152, 421, 451, 1071, 1241
BstSLI GKGCMC 1 cut(s) 1309
BstUI CGCG 1 cut(s) 573
BstV1I GCAGC 3 cut(s) 61, 867, 1075
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 5 cut(s) 152, 570, 888, 1247, 1326
BtgI CCRYGG 3 cut(s) 219, 571, 1275
BtsCI GGATG 4 cut(s) 172, 474, 1211, 1327
BtsIMutI CAGTG 4 cut(s) 246, 414, 898, 1021
BveI ACCTGC 1 cut(s) 1191
Cac8I GCNNGC 6 cut(s) 189, 308, 341, 604, 665, 1234
CaiI CAGNNNCTG 1 cut(s) 1301
CciI TCATGA 1 cut(s) 543
Cfr13I GGNCC 3 cut(s) 753, 886, 1245
Cfr42I CCGCGG 1 cut(s) 574
CseI GACGC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 456
CviQI GTAC 1 cut(s) 456
DdeI CTNAG 3 cut(s) 84, 162, 249
DpnI GATC 3 cut(s) 89, 435, 1171
DpnII GATC 3 cut(s) 87, 433, 1169
EaeI YGGCCR 2 cut(s) 150, 568
Eam1104I CTCTTC 2 cut(s) 465, 1148
EarI CTCTTC 2 cut(s) 465, 1148
EciI GGCGGA 1 cut(s) 730
Ecl136II GAGCTC 3 cut(s) 129, 538, 693
Eco130I CCWWGG 3 cut(s) 219, 974, 1275
Eco24I GRGCYC 5 cut(s) 131, 540, 695, 827, 1059
Eco31I GGTCTC 1 cut(s) 1173
Eco47I GGWCC 1 cut(s) 753
Eco53kI GAGCTC 3 cut(s) 129, 538, 693
Eco57I CTGAAG 2 cut(s) 1212, 1278
Eco91I GGTNACC 1 cut(s) 1117
EcoICRI GAGCTC 3 cut(s) 129, 538, 693
EcoO109I RGGNCCY 1 cut(s) 1245
EcoO65I GGTNACC 1 cut(s) 1117
EcoRII CCWGG 4 cut(s) 152, 421, 1071, 1241
EcoT14I CCWWGG 3 cut(s) 219, 974, 1275
EcoT22I ATGCAT 1 cut(s) 608
EcoT38I GRGCYC 5 cut(s) 131, 540, 695, 827, 1059
ErhI CCWWGG 3 cut(s) 219, 974, 1275
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 1 cut(s) 969
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 7 cut(s) 75, 150, 358, 571, 881, 1089, 1318
FokI GGATG 4 cut(s) 179, 461, 1198, 1314
FriOI GRGCYC 5 cut(s) 131, 540, 695, 827, 1059
Fsp4HI GCNGC 7 cut(s) 75, 150, 358, 571, 881, 1089, 1318
FspBI CTAG 1 cut(s) 1336
GluI GCNGC 7 cut(s) 75, 150, 358, 571, 881, 1089, 1318
GsaI CCCAGC 2 cut(s) 266, 887
GsuI CTGGAG 2 cut(s) 1186, 1242
HaeIII GGCC 5 cut(s) 152, 570, 888, 1247, 1326
HapII CCGG 2 cut(s) 453, 1215
HgaI GACGC 1 cut(s) 332
HincII GTYRAC 3 cut(s) 226, 442, 772
HindII GTYRAC 3 cut(s) 226, 442, 772
HindIII AAGCTT 1 cut(s) 1252
HinfI GANTC 3 cut(s) 317, 576, 896
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 2 cut(s) 453, 1215
HphI GGTGA 6 cut(s) 437, 529, 697, 743, 886, 1015
Hpy166II GTNNAC 7 cut(s) 226, 442, 586, 647, 772, 1006, 1307
Hpy188I TCNGA 3 cut(s) 239, 1174, 1191
Hpy188III TCNNGA 8 cut(s) 93, 392, 478, 493, 544, 556, 1132, 1215
Hpy8I GTNNAC 7 cut(s) 226, 442, 586, 647, 772, 1006, 1307
HpyAV CCTTC 2 cut(s) 249, 1169
HpyCH4III ACNGT 2 cut(s) 794, 893
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 58, 80, 158, 599, 1238, 1323, 1332
HpyF3I CTNAG 3 cut(s) 84, 162, 249
Kpn2I TCCGGA 1 cut(s) 1214
KspAI GTTAAC 1 cut(s) 442
KspI CCGCGG 1 cut(s) 574
Kzo9I GATC 3 cut(s) 87, 433, 1169
LguI GCTCTTC 1 cut(s) 1148
LmnI GCTCC 8 cut(s) 535, 599, 690, 1096, 1217, 1223, 1259, 1283
Lsp1109I GCAGC 3 cut(s) 61, 867, 1075
LweI GCATC 4 cut(s) 615, 724, 802, 867
MaeI CTAG 1 cut(s) 1336
MaeIII GTNAC 6 cut(s) 28, 412, 622, 893, 925, 1117
MalI GATC 3 cut(s) 89, 435, 1171
MboI GATC 3 cut(s) 87, 433, 1169
MboII GAAGA 4 cut(s) 262, 452, 739, 1165
MfeI CAATTG 4 cut(s) 78, 294, 524, 930
MflI RGATCY 1 cut(s) 87
MhlI GDGCHC 6 cut(s) 131, 540, 695, 827, 1059, 1309
MluCI AATT 8 cut(s) 78, 294, 524, 617, 636, 778, 834, 930
MlyI GAGTC 2 cut(s) 585, 890
MmeI TCCRAC 4 cut(s) 813, 870, 1118, 1261
Mph1103I ATGCAT 1 cut(s) 608
MroI TCCGGA 1 cut(s) 1214
MroXI GAANNNNTTC 1 cut(s) 551
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 2 cut(s) 654, 867
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 2 cut(s) 453, 1215
MspR9I CCNGG 5 cut(s) 154, 423, 453, 1073, 1243
MunI CAATTG 4 cut(s) 78, 294, 524, 930
Mva1269I GAATGC 1 cut(s) 975
MvaI CCWGG 4 cut(s) 154, 423, 1073, 1243
MvnI CGCG 1 cut(s) 573
MwoI GCNNNNNNNGC 8 cut(s) 44, 58, 80, 158, 599, 1238, 1323, 1332
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 2 cut(s) 219, 1275
NdeII GATC 3 cut(s) 87, 433, 1169
NlaIV GGNNCC 4 cut(s) 754, 1198, 1261, 1285
NmuCI GTSAC 3 cut(s) 412, 893, 925
NsiI ATGCAT 1 cut(s) 608
NspI RCATGY 2 cut(s) 191, 606
PaeI GCATGC 2 cut(s) 191, 606
PagI TCATGA 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 1148
PctI GAATGC 1 cut(s) 975
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 7 cut(s) 76, 151, 359, 572, 882, 1090, 1319
PleI GAGTC 2 cut(s) 584, 890
PpsI GAGTC 2 cut(s) 584, 890
Psp124BI GAGCTC 3 cut(s) 131, 540, 695
Psp6I CCWGG 4 cut(s) 152, 421, 1071, 1241
PspEI GGTNACC 1 cut(s) 1117
PspFI CCCAGC 2 cut(s) 262, 883
PspGI CCWGG 4 cut(s) 152, 421, 1071, 1241
PspN4I GGNNCC 4 cut(s) 754, 1198, 1261, 1285
PspPI GGNCC 3 cut(s) 753, 886, 1245
PsrI GAACNNNNNNTAC 2 cut(s) 998, 1030
PstNI CAGNNNCTG 1 cut(s) 1301
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
RseI CAYNNNNRTG 2 cut(s) 654, 867
SacI GAGCTC 3 cut(s) 131, 540, 695
SacII CCGCGG 1 cut(s) 574
SapI GCTCTTC 1 cut(s) 1148
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 7 cut(s) 75, 150, 358, 571, 881, 1089, 1318
Sau3AI GATC 3 cut(s) 87, 433, 1169
Sau96I GGNCC 3 cut(s) 753, 886, 1245
SchI GAGTC 2 cut(s) 585, 890
ScrFI CCNGG 5 cut(s) 154, 423, 453, 1073, 1243
SduI GDGCHC 6 cut(s) 131, 540, 695, 827, 1059, 1309
SfaNI GCATC 4 cut(s) 615, 724, 802, 867
Sfr303I CCGCGG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 574
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 2 cut(s) 654, 867
SmlI CTYRAG 2 cut(s) 130, 1249
SmoI CTYRAG 2 cut(s) 130, 1249
SphI GCATGC 2 cut(s) 191, 606
Sse9I AATT 8 cut(s) 78, 294, 524, 617, 636, 778, 834, 930
SsiI CCGC 9 cut(s) 5, 47, 149, 337, 357, 571, 573, 715, 1318
SspMI CTAG 1 cut(s) 1336
SstI GAGCTC 3 cut(s) 131, 540, 695
StyD4I CCNGG 5 cut(s) 152, 421, 451, 1071, 1241
StyI CCWWGG 3 cut(s) 219, 974, 1275
TaaI ACNGT 2 cut(s) 794, 893
TaqI TCGA 1 cut(s) 1131
TasI AATT 8 cut(s) 78, 294, 524, 617, 636, 778, 834, 930
TauI GCSGC 4 cut(s) 152, 360, 573, 1320
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 4 cut(s) 253, 421, 898, 1028
TseFI GTSAC 3 cut(s) 412, 893, 925
TseI GCWGC 3 cut(s) 74, 880, 1088
Tsp45I GTSAC 3 cut(s) 412, 893, 925
TspDTI ATGAA 2 cut(s) 560, 629
TspRI CASTG 4 cut(s) 253, 421, 898, 1028
VneI GTGCAC 1 cut(s) 1305
VpaK11BI GGWCC 1 cut(s) 753
XceI RCATGY 2 cut(s) 191, 606
XmiI GTMKAC 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 1 cut(s) 1336
Zsp2I ATGCAT 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.