MD16G1236000.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010648
Physical Location & Seq
Forward (+)
24622603 .. 24626539
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1236000.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGCGGTGTTTGATAGTAAGAAGAAATGGGTCGGTGCGTTCATGATGGTGCTTCTCATGTTCTACTTCGCTGCTGCTGCAGTGTCTGAGATCTCTCGCAACAGGAATGGAGGAAGAGACAAGTTGCAGAGCTCGAGCGACTCATCAATGGCAGCCAGAGTGGCTGAGGATGACCAAGGATTCAACAAGCATGCAGTTGAAGACCCAGAAGAGATTGCTTCCATGGTTGACATGAGTATCCGAAACAGCACTGAGAGGAGGAAGCTGGGCTACTTCTCCTGTGGAACTGGCAATCCAGTCGATGACTGCTGGCGCTGTGATCCCAACTGGCACAAAAACCGCAAGCGCCTCGCAGACTGTGGCATTGGTTTTGGCAGGAATGCCATTGGTGGTCGCGATGGACGCTTCTATGTTGTCACCGACCCTAATGATGATGACCCCGTTAACCCCAGGGCTGGCACTCTTCGCCATGCTGTCATCCAGAGCGAGCCTCTCTGGATTGTGTTCAAGCGAGACATGGTGATACAGTTGAAGCAGGAGCTCATCATGAACAGCTTCAAGACCATTGATGGACGCGGAGTCAATGTTCACATTGCTAACGGAGGATGCATCACAATCCAATATGTTACAAATATTATCATTCACGGTCTCCACATCCACGACTGCAAGCCCACAGGAAATGCTTTGGTGAGGAGCTCGCCTACTCACTTTGGGTGGCGGACAATGGCTGATGGCGATGCTGTCTCCATCTTCGGGTCAAGCCATATTTGGGTTGATCACAATTCCCTCTCCAACTGCGCTGACGGTCTAGTTGATGCTGTCATGGGCTCAACTGCAATCACCATTTCCAACAACCACATGACCCACCACAATGAGGTGATGCTGCTGGGCCACAGTGATAGCTACACCAGAGACAAGCAAATGCAAGTCACAATTGCTTACAACCACTTTGGAGAGGGACTTATCCAAAGAATGCCAAGGTGCAGGCACGGGTATTTCCATGTTGTGAACAACGACTACACTCACTGGGAAATGTATGCCATTGGTGGAAGTGGAAATCCCACCATCAACAGCCAAGGCAACAGATATGCTGCTCCAACCAACCCTTTTGCAAAGGAGGTTACCAAGAGGGTTGAGACGGCTACCACTGAATGGAAGAACTGGAACTGGAGATCAGAAGGAGACCTGCTGCTCAATGGTGCCTACTTCACTCCATCTGGAGCTGGAGCTTCAGCAAGCTACGCCAGAGCCTCAAGCTTGGGAGCCAAGTCATCTGCCATGGTTGGATCCATAACTTCAGGTTCCGGTGCACTTCCCTGCCGCAGAGGCCACCCCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

447

Amino Acids

49.14

Weight (kDa)

8.28

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 179 - 382 5.7e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24230 AT4G13710 AT4G13710
fragaria_vesca FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760 FvH4_4g05760
malus_domestica MD13G1231000.v1.1 MD16G1236000.v1.1
prunus_persica Prupe.1G060900_v2.0.a1
pyrus_communis pycom13g20360 pycom16g19790
rosa_chinensis RchiOBHm_Chr4g0396921
rosa_laevigata RLG00000009489
rosa_roxburghii Rroxscaffold_5G00341840
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075300 Rh4DG069100
rosa_wichuraiana Rw4G006040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1194
AccB1I GGYRCC 1 cut(s) 1199
AccB7I CCANNNNNTGG 1 cut(s) 1152
AccII CGCG 2 cut(s) 396, 576
AciI CCGC 5 cut(s) 5, 340, 576, 718, 1321
AclWI GGATC 3 cut(s) 314, 1281, 1294
AcuI CTGAAG 2 cut(s) 1215, 1281
AfiI CCNNNNNNNGG 5 cut(s) 455, 753, 769, 874, 1152
AgsI TTSAA 5 cut(s) 184, 200, 508, 532, 559
AhdI GACNNNNNGTC 1 cut(s) 578
AjnI CCWGG 1 cut(s) 449
AloI GAACNNNNNNTCC 4 cut(s) 277, 309, 570, 602
Alw21I GWGCWC 4 cut(s) 134, 543, 698, 1312
Alw26I GTCTC 7 cut(s) 111, 507, 653, 748, 906, 1130, 1176
Alw44I GTGCAC 1 cut(s) 1308
AlwI GGATC 3 cut(s) 314, 1281, 1294
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 2 cut(s) 889, 1327
ApaLI GTGCAC 1 cut(s) 1308
ApeKI GCWGC 7 cut(s) 71, 74, 77, 152, 883, 1091, 1189
Asp700I GAANNNNTTC 1 cut(s) 554
AspLEI GCGC 3 cut(s) 315, 348, 800
AspS9I GGNCC 1 cut(s) 889
AsuHPI GGTGA 5 cut(s) 409, 532, 700, 832, 889
AvaI CYCGRG 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1312
BaeI ACNNNNGTAYC 2 cut(s) 220, 253
BamHI GGATCC 1 cut(s) 1286
BanI GGYRCC 1 cut(s) 1199
BanII GRGCYC 4 cut(s) 134, 543, 698, 830
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 4 cut(s) 134, 543, 698, 1312
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 7 cut(s) 58, 61, 64, 164, 870, 1078, 1176
BccI CCATC 7 cut(s) 40, 392, 563, 725, 755, 1073, 1222
BceAI ACGGC 1 cut(s) 1155
BcgI CGANNNNNNTGC 4 cut(s) 280, 314, 331, 365
BciT130I CCWGG 1 cut(s) 451
BciVI GTATCC 1 cut(s) 248
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 7 cut(s) 111, 507, 653, 748, 906, 1130, 1176
BfaI CTAG 2 cut(s) 809, 1339
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 2 cut(s) 316, 349
BfuAI ACCTGC 1 cut(s) 1194
BfuI GTATCC 1 cut(s) 248
BglI GCCNNNNNGGC 2 cut(s) 161, 1326
BglII AGATCT 1 cut(s) 90
BisI GCNGC 8 cut(s) 72, 75, 78, 153, 884, 1092, 1190, 1321
BlsI GCNGC 8 cut(s) 73, 76, 79, 154, 885, 1093, 1191, 1322
Bme1390I CCNGG 1 cut(s) 451
BmeRI GACNNNNNGTC 1 cut(s) 578
BmeT110I CYCGRG 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
BmrFI CCNGG 1 cut(s) 451
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 5 cut(s) 596, 618, 727, 805, 870
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 475, 507
BpmI CTGGAG 3 cut(s) 1189, 1239, 1245
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 1237
BsaI GGTCTC 2 cut(s) 653, 1176
BsaJI CCNNGG 7 cut(s) 175, 222, 449, 450, 977, 1075, 1278
BsaWI WCCGGW 1 cut(s) 1304
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 5 cut(s) 455, 753, 769, 874, 1152
Bse1I ACTGG 6 cut(s) 292, 296, 332, 1031, 1166, 1172
Bse3DI GCAATG 1 cut(s) 591
BseBI CCWGG 1 cut(s) 451
BseDI CCNNGG 7 cut(s) 175, 222, 449, 450, 977, 1075, 1278
BseGI GGATG 4 cut(s) 175, 477, 611, 654
BseLI CCNNNNNNNGG 5 cut(s) 455, 753, 769, 874, 1152
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 3 cut(s) 78, 156, 243
BseNI ACTGG 6 cut(s) 292, 296, 332, 1031, 1166, 1172
BseRI GAGGAG 2 cut(s) 271, 706
BseSI GKGCMC 1 cut(s) 1312
BseXI GCAGC 7 cut(s) 58, 61, 64, 164, 870, 1078, 1176
BseYI CCCAGC 2 cut(s) 265, 886
BsgI GTGCAG 1 cut(s) 1003
Bsh1236I CGCG 2 cut(s) 396, 576
BshFI GGCC 2 cut(s) 891, 1329
BshNI GGYRCC 1 cut(s) 1199
BsiHKAI GWGCWC 4 cut(s) 134, 543, 698, 1312
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 1 cut(s) 1305
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 5 cut(s) 455, 753, 769, 874, 1152
BsmAI GTCTC 7 cut(s) 111, 507, 653, 748, 906, 1130, 1176
BsmBI CGTCTC 1 cut(s) 1130
BsmFI GGGAC 1 cut(s) 972
BsmI GAATGC 2 cut(s) 385, 978
BsnI GGCC 2 cut(s) 891, 1329
Bso31I GGTCTC 2 cut(s) 653, 1176
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
Bsp143I GATC 5 cut(s) 90, 319, 775, 1172, 1286
Bsp19I CCATGG 2 cut(s) 222, 1278
Bsp68I TCGCGA 1 cut(s) 396
BspACI CCGC 5 cut(s) 5, 340, 576, 718, 1321
BspANI GGCC 2 cut(s) 891, 1329
BspCNI CTCAG 3 cut(s) 79, 157, 244
BspFNI CGCG 2 cut(s) 396, 576
BspHI TCATGA 2 cut(s) 42, 546
BspLI GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
BspMAI CTGCAG 1 cut(s) 82
BspMI ACCTGC 1 cut(s) 1194
BspPI GGATC 3 cut(s) 314, 1281, 1294
BspT107I GGYRCC 1 cut(s) 1199
BspTNI GGTCTC 2 cut(s) 653, 1176
BsrDI GCAATG 1 cut(s) 591
BsrI ACTGG 6 cut(s) 292, 296, 332, 1031, 1166, 1172
BssECI CCNNGG 7 cut(s) 175, 222, 449, 450, 977, 1075, 1278
BssMI GATC 5 cut(s) 90, 319, 775, 1172, 1286
BssT1I CCWWGG 5 cut(s) 175, 222, 977, 1075, 1278
Bst2UI CCWGG 1 cut(s) 451
Bst4CI ACNGT 5 cut(s) 359, 528, 647, 806, 896
Bst6I CTCTTC 3 cut(s) 109, 204, 468
BstC8I GCNNGC 9 cut(s) 192, 311, 344, 457, 488, 668, 698, 986, 1237
BstDEI CTNAG 3 cut(s) 87, 165, 252
BstDSI CCRYGG 2 cut(s) 222, 1278
BstEII GGTNACC 1 cut(s) 1120
BstF5I GGATG 4 cut(s) 175, 477, 611, 654
BstFNI CGCG 2 cut(s) 396, 576
BstH2I RGCGCY 2 cut(s) 316, 349
BstHHI GCGC 3 cut(s) 315, 348, 800
BstKTI GATC 5 cut(s) 93, 322, 778, 1175, 1289
BstMAI GTCTC 7 cut(s) 111, 507, 653, 748, 906, 1130, 1176
BstMBI GATC 5 cut(s) 90, 319, 775, 1172, 1286
BstMWI GCNNNNNNNGC 7 cut(s) 77, 161, 402, 465, 1241, 1326, 1335
BstNI CCWGG 1 cut(s) 451
BstNSI RCATGY 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 1120
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 1312
BstUI CGCG 2 cut(s) 396, 576
BstV1I GCAGC 7 cut(s) 58, 61, 64, 164, 870, 1078, 1176
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 2 cut(s) 90, 1286
BstYI RGATCY 2 cut(s) 90, 1286
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 2 cut(s) 891, 1329
BtgI CCRYGG 2 cut(s) 222, 1278
BtgZI GCGATG 2 cut(s) 411, 750
BtsCI GGATG 4 cut(s) 175, 477, 611, 654
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 5 cut(s) 87, 249, 901, 1024, 1146
BtuMI TCGCGA 1 cut(s) 396
BveI ACCTGC 1 cut(s) 1194
Cac8I GCNNGC 9 cut(s) 192, 311, 344, 457, 488, 668, 698, 986, 1237
CaiI CAGNNNCTG 1 cut(s) 86
CciI TCATGA 2 cut(s) 42, 546
CfoI GCGC 3 cut(s) 315, 348, 800
Cfr13I GGNCC 1 cut(s) 889
CseI GACGC 2 cut(s) 411, 582
DdeI CTNAG 3 cut(s) 87, 165, 252
DpnI GATC 5 cut(s) 92, 321, 777, 1174, 1288
DpnII GATC 5 cut(s) 90, 319, 775, 1172, 1286
DriI GACNNNNNGTC 1 cut(s) 578
Eam1104I CTCTTC 3 cut(s) 109, 204, 468
Eam1105I GACNNNNNGTC 1 cut(s) 578
EarI CTCTTC 3 cut(s) 109, 204, 468
EciI GGCGGA 1 cut(s) 733
Ecl136II GAGCTC 3 cut(s) 132, 541, 696
Eco130I CCWWGG 5 cut(s) 175, 222, 977, 1075, 1278
Eco24I GRGCYC 4 cut(s) 134, 543, 698, 830
Eco31I GGTCTC 2 cut(s) 653, 1176
Eco53kI GAGCTC 3 cut(s) 132, 541, 696
Eco57I CTGAAG 2 cut(s) 1215, 1281
Eco88I CYCGRG 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 1120
EcoICRI GAGCTC 3 cut(s) 132, 541, 696
EcoO65I GGTNACC 1 cut(s) 1120
EcoRII CCWGG 1 cut(s) 449
EcoT14I CCWWGG 5 cut(s) 175, 222, 977, 1075, 1278
EcoT22I ATGCAT 1 cut(s) 611
EcoT38I GRGCYC 4 cut(s) 134, 543, 698, 830
ErhI CCWWGG 5 cut(s) 175, 222, 977, 1075, 1278
Esp3I CGTCTC 1 cut(s) 1130
FaqI GGGAC 1 cut(s) 972
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 8 cut(s) 72, 75, 78, 153, 884, 1092, 1190, 1321
FokI GGATG 4 cut(s) 182, 464, 618, 641
FriOI GRGCYC 4 cut(s) 134, 543, 698, 830
Fsp4HI GCNGC 8 cut(s) 72, 75, 78, 153, 884, 1092, 1190, 1321
FspBI CTAG 2 cut(s) 809, 1339
GlaI GCGC 3 cut(s) 314, 347, 799
GluI GCNGC 8 cut(s) 72, 75, 78, 153, 884, 1092, 1190, 1321
GsaI CCCAGC 2 cut(s) 269, 890
GsuI CTGGAG 3 cut(s) 1189, 1239, 1245
HaeII RGCGCY 2 cut(s) 316, 349
HaeIII GGCC 2 cut(s) 891, 1329
HapII CCGG 1 cut(s) 1305
HgaI GACGC 2 cut(s) 411, 582
HhaI GCGC 3 cut(s) 315, 348, 800
Hin6I GCGC 3 cut(s) 313, 346, 798
HinP1I GCGC 3 cut(s) 313, 346, 798
HincII GTYRAC 2 cut(s) 229, 445
HindII GTYRAC 2 cut(s) 229, 445
HindIII AAGCTT 1 cut(s) 1255
HinfI GANTC 3 cut(s) 140, 180, 579
HpaI GTTAAC 1 cut(s) 445
HpaII CCGG 1 cut(s) 1305
HphI GGTGA 5 cut(s) 409, 532, 700, 832, 889
Hpy166II GTNNAC 5 cut(s) 229, 445, 589, 1009, 1310
Hpy188I TCNGA 3 cut(s) 88, 242, 1177
Hpy188III TCNNGA 7 cut(s) 43, 395, 481, 496, 547, 559, 1218
Hpy8I GTNNAC 5 cut(s) 229, 445, 589, 1009, 1310
HpyAV CCTTC 1 cut(s) 1172
HpyCH4III ACNGT 5 cut(s) 359, 528, 647, 806, 896
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 161, 402, 465, 1241, 1326, 1335
HpyF3I CTNAG 3 cut(s) 87, 165, 252
HspAI GCGC 3 cut(s) 313, 346, 798
Ksp22I TGATCA 1 cut(s) 775
KspAI GTTAAC 1 cut(s) 445
Kzo9I GATC 5 cut(s) 90, 319, 775, 1172, 1286
LmnI GCTCC 6 cut(s) 538, 693, 1099, 1220, 1226, 1262
Lsp1109I GCAGC 7 cut(s) 58, 61, 64, 164, 870, 1078, 1176
LweI GCATC 5 cut(s) 596, 618, 727, 805, 870
MaeI CTAG 2 cut(s) 809, 1339
MaeIII GTNAC 4 cut(s) 415, 625, 928, 1120
MalI GATC 5 cut(s) 92, 321, 777, 1174, 1288
MboI GATC 5 cut(s) 90, 319, 775, 1172, 1286
MboII GAAGA 7 cut(s) 34, 126, 212, 221, 455, 742, 1168
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 2 cut(s) 90, 1286
MhlI GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
MluCI AATT 2 cut(s) 781, 933
MlyI GAGTC 2 cut(s) 134, 588
MmeI TCCRAC 4 cut(s) 816, 873, 1121, 1264
Mph1103I ATGCAT 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 2 cut(s) 47, 870
MspI CCGG 1 cut(s) 1305
MspR9I CCNGG 1 cut(s) 451
MunI CAATTG 1 cut(s) 933
Mva1269I GAATGC 2 cut(s) 385, 978
MvaI CCWGG 1 cut(s) 451
MvnI CGCG 2 cut(s) 396, 576
MwoI GCNNNNNNNGC 7 cut(s) 77, 161, 402, 465, 1241, 1326, 1335
NcoI CCATGG 2 cut(s) 222, 1278
NdeII GATC 5 cut(s) 90, 319, 775, 1172, 1286
NlaIV GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
NmuCI GTSAC 2 cut(s) 415, 928
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 611
NspI RCATGY 1 cut(s) 194
PaeI GCATGC 1 cut(s) 194
PaeR7I CTCGAG 1 cut(s) 133
PagI TCATGA 2 cut(s) 42, 546
PasI CCCWGGG 1 cut(s) 450
PctI GAATGC 2 cut(s) 385, 978
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 180
PflMI CCANNNNNTGG 1 cut(s) 1152
PkrI GCNGC 8 cut(s) 73, 76, 79, 154, 885, 1093, 1191, 1322
PleI GAGTC 2 cut(s) 134, 587
PpsI GAGTC 2 cut(s) 134, 587
Psp124BI GAGCTC 3 cut(s) 134, 543, 698
Psp6I CCWGG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 1120
PspFI CCCAGC 2 cut(s) 265, 886
PspGI CCWGG 1 cut(s) 449
PspN4I GGNNCC 4 cut(s) 1201, 1264, 1288, 1303
PspPI GGNCC 1 cut(s) 889
PspXI VCTCGAGB 1 cut(s) 133
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PstI CTGCAG 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 2 cut(s) 90, 1286
RruI TCGCGA 1 cut(s) 396
RseI CAYNNNNRTG 2 cut(s) 47, 870
SacI GAGCTC 3 cut(s) 134, 543, 698
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 8 cut(s) 72, 75, 78, 153, 884, 1092, 1190, 1321
Sau3AI GATC 5 cut(s) 90, 319, 775, 1172, 1286
Sau96I GGNCC 1 cut(s) 889
SchI GAGTC 2 cut(s) 134, 588
ScrFI CCNGG 1 cut(s) 451
SduI GDGCHC 5 cut(s) 134, 543, 698, 830, 1312
SfaNI GCATC 5 cut(s) 596, 618, 727, 805, 870
SfcI CTRYAG 1 cut(s) 78
Sfr274I CTCGAG 1 cut(s) 133
SlaI CTCGAG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 47, 870
SmlI CTYRAG 2 cut(s) 133, 1252
SmoI CTYRAG 2 cut(s) 133, 1252
SphI GCATGC 1 cut(s) 194
Sse9I AATT 2 cut(s) 781, 933
SsiI CCGC 5 cut(s) 5, 340, 576, 718, 1321
SspI AATATT 1 cut(s) 634
SspMI CTAG 2 cut(s) 809, 1339
SstI GAGCTC 3 cut(s) 134, 543, 698
StyD4I CCNGG 1 cut(s) 449
StyI CCWWGG 5 cut(s) 175, 222, 977, 1075, 1278
TaaI ACNGT 5 cut(s) 359, 528, 647, 806, 896
TaqI TCGA 2 cut(s) 134, 300
TasI AATT 2 cut(s) 781, 933
TauI GCSGC 1 cut(s) 1323
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 5 cut(s) 87, 256, 901, 1031, 1153
TseFI GTSAC 2 cut(s) 415, 928
TseI GCWGC 7 cut(s) 71, 74, 77, 152, 883, 1091, 1189
Tsp45I GTSAC 2 cut(s) 415, 928
TspDTI ATGAA 2 cut(s) 31, 563
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 5 cut(s) 87, 256, 901, 1031, 1153
Van91I CCANNNNNTGG 1 cut(s) 1152
VneI GTGCAC 1 cut(s) 1308
XceI RCATGY 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 2 cut(s) 809, 1339
Zsp2I ATGCAT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.