FvH4_4g12350
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016035
Physical Location & Seq
Reverse (-)
16038563 .. 16042271
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12350.t1

Sequence Viewer

Length: 3021 bp
ATGGCTTTTCTCAGATTTCTCATATACACACTAATGGCTTCTCTGCAACTCTGCATGGGCGACGACCCCATCCCGGTGCAGCTCAACTACGACGTGCTCGGCCTCCTCGTCTTCAAATCCGACCTCCACGACCCTTCTTCCTACCTCTCTTCCTGGAACGAAGACGACGACTCCCCTTGCTCATGGAACTTCATCCAATGCAACCCTTCCACCGGAAGAGTCTCCCAACTCAACCTCGACGGCTTAGGCCTCAAGGGCAAACCCGGTAAAGGTCTTCAGAATCTTCAGCACCTCAAGGTACTCTCTTTATCCGGCAACAACTTCACTGGTGAGCTTAGTCCAGAGAAGCTTGCTCTCCCTCCAAGTCTCCAAACACTCAACTTAAGCCGGAACAGTTTCTCGGGACTAGTTCCCACTACTCTTGTCAACTTCAGCTCCATTAGATTTCTTGACCTGTCTCAAAACTCACTGTCTGGTTCACTCCCAGATTCTCTCTTTGGTGCTTGCTCTTCGCTTCGCTACCTTTCCTTATCTGGAAACTTGCTAGAAGGAAACTTGCCTAGTACTCTTTCTAAATGCTCCTCTTTGAATAGCCTCAACATCTCCAACAACCGTTTCTCCGGTAACCCGGATTTCGAATCCGGGATTTGGTCCTTGAAACGCCTTCGTTCTCTGGATCTTTCAAACAATGCTCTATCCGGGTTCGTGCCGAAAGGGATATCATCTATACATAATTTGAAAGAGATTTTGATTCAGAGGAACCATTTTTCTGGGACAATCCCTTTTGATATTGGACTGTGTCCTCACTTGGGTCGGATCGATTTCAGTGAAAATCTCTTCACTGGTGAGTTGCCACAGTCACTTCAGATGCTGAATTTCCTGACATTTATGAGTCTTTCAGATAATATGTTCAATGGTGATTTTCCTCAATGGATTGGGAACTTGAGTAGCCTTGAGTACTTGGACTTTTCCAACAATGGCTTCACTGGAAGCCTCCCACCATCAATGGGTGACCTTAGATCTCTCAGTTATTTAAGTTTGTCTAACAACAAACTTGTTGGGACACTTCCAACTTCTCTTGTCTACTGCAACAAGTTGTCTGTGATTCGGTTGAGGGGTAATGATTTCAGTGGGAGCATACCAGAAGGTTTGTTTGATCTTGGGTTGGAAGAGATAGACTTTTCGAATATGGGACTAACAGGTTCAATCCCACCCGGTTCGAGTAAATTGTTCGAGTCTTTGAAGATGTTGGATCTGTCAAGGAACAATCTCAAAGGCAACATTCCAGCAGAGGTGGGACTTTTCTCCAACTTGAGGTACTTGAATTTTTCATGGAACAATCTTCAATCAAGAATTCCACCAGAGCTAGGATTTTTCTCGAATCTCACTGTGTTAGATCTACGGAATAGTGCATTGTCTGGTCCAATCCCAGGAGAAATATGTGACTCTGGTAGTTTGGATATTCTCCAATTGGATGGAAATTCATTGACTGGCCCAATTCCTGATGAGATTGGGAATTGCTCATCTCTCTATTTGATGAGTTTGTCACATAACAATTTAAGTGGTGTTATTCCAAAATCCATTTCGAAGTTGGGCAAGCTCGTGATTCTCAAGTTAGAGTTCAATGAGTTGAGTGGAGAGATACCACTAGAGCTTGGAAAATTGGAAAATCTTCTTGCTGTGAACATTTCATATAACAGATTAACAGGGAGGCTTCCTGTTGGGAGTGTGTTTCAGAGTTTGGATCAAAGTGCTTTGCAAGGAAACTTGGGTATTTGTTCACCATTGTTGAAGGGTCCATGTACAATGAATGTACCTAAGCCTCTTGTTCTTGATCCAAATGCCTACCCTAACCAAATGGGTGGTGGAGATCATAGATACCATGGCGACTCATCAGAGTCTCGAAAAGGACATCACCATATGTTCCTTAGCATATCTGCTATTGTGGCGATTTCAGCTGCTACTCTGATTGCTGTTGGAGTAATAGTCATTAGCCTTCTGAATGCATCTGCAAGAAGGAGGCCTGCATTTGTGGAAACTGCCTTGGAAAGCATGTGCTCGATGTCTTCGCGATCGGGTAGTTTGGCCTCAGGGAAGCTCATTCTGTTTGATTCAAGGTCATCCCCGGATTGGATCAGCAGTCCTGAATCACTATTGAACAAGGCCTCTGAGCTTGGGGAGGGAGTCTTTGGAACAGTCTACAAAGTTCCACTGGGAGCTCAGGGAAGAATGGTGGCAATCAAAAAGCTTGTGACAACAAACATACTCCAATGTCTTGAAGACTTTGACAGAGAAGTTCGAGTCCTTGGAAAGGCAAGGCACCCAAATCTAGTAGCCCTGAAAGGATACTACTGGACTCCACAGATGCAGCTACTTGTGAATGAGTATGCCCCCAATGGCAGCCTACAATCCAAACTCCATGATAGGCTTTATTCAAGTTCACCTCTTTCATGGGATGATAGGTTTAAAATCCTGCTAGGAACAGCAAAGGGACTTTCCCACTTGCACCATTCTTTTCGCCCTCCTATCATTCACTACAACGTAAAACCAAGCAACATACTGCTCGATGAAGACCTCAACCCGAAGATTTCAGATTTTGCACTTGCAAGGCTACTAACCAAGATCGACAGGCATGTAGTCAGTAACCGATTTCAGAGTGCATTGGGCTATGTGGCGCCGGAACTAGCTTGCCAGAGCCTAAGGGTGAATGAGAAATGCGATGTGTATGGTTTTGGCGTGTTGATCCTTGAGCTTGTGACTGGTAGAAGGCCAGTGGAATATGGGGAAGACAATGTGGTGATATTAAACGATCATGTGAAGGTTTTACTTGAACAAGGCAATGTGCTAGGATGCATTGATGTTAGCATGGGGGAGTACCCAGAAGATGAAGTCCTACCAGTGCTCAAGCTGGCCTTGGTGTGCACTTCTCAGATACCATCTTGTAGGCCAACCATGGCAGAAGTGGTGCAAATTCTTCAGATTATCAAGACTCCACTTCCACAAAGAATTGAGCGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1007

Amino Acids

110.04

Weight (kDa)

6.13

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 1.7e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 76 - 132 5.5e-06 Leucine rich repeat
LRR_14 PF23598 90 - 298 1.9e-07 Leucine-rich repeat region
LRR_8 PF13855 97 - 157 2e-08 Leucine rich repeat
LRR_8 PF13855 160 - 206 5.1e-06 Leucine rich repeat
LRR_8 PF13855 297 - 352 4.7e-07 Leucine rich repeat
LRR_14 PF23598 409 - 564 5.7e-11 Leucine-rich repeat region
Pkinase PF00069 721 - 990 3.5e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 721 - 991 2.5e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28040
fragaria_vesca FvH4_4g12350
malus_domestica MD04G1027600.v1.1
prunus_persica Prupe.1G177000_v2.0.a1
pyrus_communis pycom04g02420
rosa_chinensis RchiOBHm_Chr4g0410441
rosa_laevigata RLG00000008430
rosa_multiflora Rmu_sc0002111.1_g000005
rosa_roxburghii Rroxscaffold_5G00355250
rosa_rugosa Rorug04G0102700
rosa_samantha Rh4AG160700 Rh4BG159900 Rh4CG172800 Rh4DG155600
rosa_wichuraiana Rw4G013390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2319, 2674
AccI GTMKAC 2 cut(s) 1083, 2199
AccII CGCG 1 cut(s) 2071
AclWI GGATC 7 cut(s) 684, 824, 1260, 1752, 1829, 2141, 2737
AcsI RAATTY 5 cut(s) 874, 1324, 1353, 1480, 2970
AcuI CTGAAG 5 cut(s) 260, 269, 415, 848, 2960
AcyI GRCGYC 1 cut(s) 2675
AfaI GTAC 7 cut(s) 300, 565, 959, 1319, 1804, 1815, 2876
AflII CTTAAG 1 cut(s) 382
AhlI ACTAGT 1 cut(s) 406
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 2 cut(s) 152, 1429
AjuI GAANNNNNNNTTGG 6 cut(s) 599, 631, 965, 997, 1567, 1599
Alw21I GWGCWC 5 cut(s) 99, 2060, 2221, 2904, 2924
Alw26I GTCTC 4 cut(s) 226, 371, 462, 1905
Alw44I GTGCAC 1 cut(s) 2920
AlwI GGATC 7 cut(s) 684, 824, 1260, 1752, 1829, 2141, 2737
AlwNI CAGNNNCTG 1 cut(s) 871
Ama87I CYCGRG 1 cut(s) 400
AoxI GGCC 9 cut(s) 100, 247, 1492, 2021, 2085, 2163, 2768, 2910, 2945
ApaLI GTGCAC 1 cut(s) 2920
ApeKI GCWGC 4 cut(s) 79, 1958, 2368, 2400
ApoI RAATTY 5 cut(s) 874, 1324, 1353, 1480, 2970
Asp700I GAANNNNTTC 2 cut(s) 395, 1671
AspLEI GCGC 1 cut(s) 2677
AspS9I GGNCC 4 cut(s) 651, 1421, 1493, 1796
AsuC2I CCSGG 7 cut(s) 74, 264, 629, 643, 700, 1215, 2126
AsuHPI GGTGA 9 cut(s) 341, 857, 929, 1022, 1773, 1907, 2433, 2716, 2809
AsuII TTCGAA 3 cut(s) 636, 1184, 1586
AvaI CYCGRG 1 cut(s) 400
AvaII GGWCC 3 cut(s) 651, 1421, 1796
AxyI CCTNAGG 2 cut(s) 2089, 2699
BaeGI GKGCMC 1 cut(s) 2924
BanI GGYRCC 2 cut(s) 2319, 2674
BanII GRGCYC 1 cut(s) 2221
BauI CACGAG 1 cut(s) 1601
BbsI GAAGAC 7 cut(s) 103, 168, 266, 2058, 2286, 2577, 2793
Bbv12I GWGCWC 5 cut(s) 99, 2060, 2221, 2904, 2924
BbvI GCAGC 4 cut(s) 91, 1945, 2380, 2412
BccI CCATC 4 cut(s) 77, 1009, 1469, 2944
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 2 cut(s) 154, 1431
BciVI GTATCC 1 cut(s) 2339
BcnI CCSGG 7 cut(s) 74, 264, 629, 643, 700, 1215, 2126
BcoDI GTCTC 4 cut(s) 226, 371, 462, 1905
BcuI ACTAGT 1 cut(s) 406
BfaI CTAG 9 cut(s) 407, 545, 561, 1367, 1649, 2330, 2477, 2684, 2846
BfoI RGCGCY 1 cut(s) 2678
BfrI CTTAAG 1 cut(s) 382
BfuI GTATCC 1 cut(s) 2339
BglI GCCNNNNNGGC 1 cut(s) 255
BglII AGATCT 2 cut(s) 1019, 1396
BisI GCNGC 4 cut(s) 80, 1959, 2369, 2401
BlsI GCNGC 4 cut(s) 81, 1960, 2370, 2402
BmcAI AGTACT 2 cut(s) 565, 959
Bme1390I CCNGG 9 cut(s) 74, 154, 264, 629, 643, 700, 1215, 1431, 2126
Bme18I GGWCC 3 cut(s) 651, 1421, 1796
BmeT110I CYCGRG 1 cut(s) 400
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 4 cut(s) 651, 1421, 1493, 1796
BmiI GGNNCC 4 cut(s) 761, 1797, 2321, 2676
BmrFI CCNGG 9 cut(s) 74, 154, 264, 629, 643, 700, 1215, 1431, 2126
BmrI ACTGGG 1 cut(s) 2222
BmsI GCATC 4 cut(s) 858, 2015, 2355, 2840
BmuI ACTGGG 1 cut(s) 2222
BpiI GAAGAC 7 cut(s) 103, 168, 266, 2058, 2286, 2577, 2793
Bpu10I CCTNAGC 4 cut(s) 244, 1818, 1928, 2220
Bpu14I TTCGAA 3 cut(s) 636, 1184, 1586
BpuEI CTTGAG 8 cut(s) 236, 278, 964, 974, 1333, 1595, 2768, 2888
BpuMI CCSGG 7 cut(s) 74, 264, 629, 643, 700, 1215, 2126
Bsa29I ATCGAT 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 2675
BsaJI CCNNGG 7 cut(s) 1429, 1882, 2043, 2124, 2305, 2913, 2952
BsaWI WCCGGW 2 cut(s) 212, 620
BsaXI ACNNNNNCTCC 8 cut(s) 155, 185, 419, 449, 602, 632, 1971, 2001
Bse1I ACTGG 9 cut(s) 331, 847, 991, 1495, 2217, 2357, 2764, 2771, 2897
Bse21I CCTNAGG 2 cut(s) 2089, 2699
Bse3DI GCAATG 1 cut(s) 2845
BseBI CCWGG 2 cut(s) 154, 1431
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 7 cut(s) 1429, 1882, 2043, 2124, 2305, 2913, 2952
BseGI GGATG 6 cut(s) 69, 192, 1480, 2120, 2461, 2855
BseMI GCAATG 1 cut(s) 2845
BseMII CTCAG 6 cut(s) 25, 1039, 2103, 2160, 2234, 2942
BseNI ACTGG 9 cut(s) 331, 847, 991, 1495, 2217, 2357, 2764, 2771, 2897
BseRI GAGGAG 2 cut(s) 95, 571
BseSI GKGCMC 1 cut(s) 2924
BseXI GCAGC 4 cut(s) 91, 1945, 2380, 2412
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 2071
Bsh1285I CGRYCG 1 cut(s) 2075
BshFI GGCC 9 cut(s) 102, 249, 1494, 2023, 2087, 2165, 2770, 2912, 2947
BshNI GGYRCC 2 cut(s) 2319, 2674
BshVI ATCGAT 1 cut(s) 819
BsiEI CGRYCG 1 cut(s) 2075
BsiHKAI GWGCWC 5 cut(s) 99, 2060, 2221, 2904, 2924
BsiHKCI CYCGRG 1 cut(s) 400
BslFI GGGAC 6 cut(s) 417, 787, 1075, 1206, 1311, 2505
BsmAI GTCTC 4 cut(s) 226, 371, 462, 1905
BsmFI GGGAC 6 cut(s) 417, 787, 1075, 1206, 1311, 2505
BsmI GAATGC 1 cut(s) 2008
BsnI GGCC 9 cut(s) 102, 249, 1494, 2023, 2087, 2165, 2770, 2912, 2947
BsoBI CYCGRG 1 cut(s) 400
Bsp119I TTCGAA 3 cut(s) 636, 1184, 1586
Bsp1286I GDGCHC 5 cut(s) 99, 2060, 2221, 2904, 2924
Bsp1407I TGTACA 1 cut(s) 1802
Bsp19I CCATGG 2 cut(s) 1882, 2952
Bsp68I TCGCGA 1 cut(s) 2071
BspANI GGCC 9 cut(s) 102, 249, 1494, 2023, 2087, 2165, 2770, 2912, 2947
BspCNI CTCAG 6 cut(s) 24, 1038, 2102, 2161, 2233, 2941
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 1 cut(s) 2071
BspLI GGNNCC 4 cut(s) 761, 1797, 2321, 2676
BspPI GGATC 7 cut(s) 684, 824, 1260, 1752, 1829, 2141, 2737
BspQI GCTCTTC 1 cut(s) 514
BspT104I TTCGAA 3 cut(s) 636, 1184, 1586
BspT107I GGYRCC 2 cut(s) 2319, 2674
BspTI CTTAAG 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 2845
BsrGI TGTACA 1 cut(s) 1802
BsrI ACTGG 9 cut(s) 331, 847, 991, 1495, 2217, 2357, 2764, 2771, 2897
BssECI CCNNGG 7 cut(s) 1429, 1882, 2043, 2124, 2305, 2913, 2952
BssNI GRCGYC 1 cut(s) 2675
BssSI CACGAG 1 cut(s) 1601
BssT1I CCWWGG 5 cut(s) 1882, 2043, 2305, 2913, 2952
Bst2BI CACGAG 1 cut(s) 1601
Bst2UI CCWGG 2 cut(s) 154, 1431
Bst4CI ACNGT 7 cut(s) 395, 471, 614, 798, 858, 1390, 2197
Bst6I CTCTTC 5 cut(s) 154, 211, 514, 842, 1164
BstACI GRCGYC 1 cut(s) 2675
BstAFI CTTAAG 1 cut(s) 382
BstAUI TGTACA 1 cut(s) 1802
BstBI TTCGAA 3 cut(s) 636, 1184, 1586
BstC8I GCNNGC 6 cut(s) 351, 505, 1598, 2025, 2689, 2910
BstDSI CCRYGG 2 cut(s) 1882, 2952
BstEII GGTNACC 2 cut(s) 623, 1010
BstENI CCTNNNNNAGG 1 cut(s) 2309
BstF5I GGATG 6 cut(s) 69, 192, 1480, 2120, 2461, 2855
BstFNI CGCG 1 cut(s) 2071
BstH2I RGCGCY 1 cut(s) 2678
BstHHI GCGC 1 cut(s) 2677
BstMAI GTCTC 4 cut(s) 226, 371, 462, 1905
BstMCI CGRYCG 1 cut(s) 2075
BstMWI GCNNNNNNNGC 3 cut(s) 255, 1946, 1955
BstNI CCWGG 2 cut(s) 154, 1431
BstNSI RCATGY 2 cut(s) 2056, 2636
BstPI GGTNACC 2 cut(s) 623, 1010
BstSCI CCNGG 9 cut(s) 72, 152, 262, 627, 641, 698, 1213, 1429, 2124
BstSLI GKGCMC 1 cut(s) 2924
BstUI CGCG 1 cut(s) 2071
BstV1I GCAGC 4 cut(s) 91, 1945, 2380, 2412
BstV2I GAAGAC 7 cut(s) 103, 168, 266, 2058, 2286, 2577, 2793
BstX2I RGATCY 4 cut(s) 676, 1019, 1252, 1396
BstXI CCANNNNNNTGG 3 cut(s) 770, 1475, 1862
BstYI RGATCY 4 cut(s) 676, 1019, 1252, 1396
Bsu15I ATCGAT 1 cut(s) 819
Bsu36I CCTNAGG 2 cut(s) 2089, 2699
BsuI GTATCC 1 cut(s) 2339
BsuRI GGCC 9 cut(s) 102, 249, 1494, 2023, 2087, 2165, 2770, 2912, 2947
BsuTUI ATCGAT 1 cut(s) 819
BtgI CCRYGG 2 cut(s) 1882, 2952
BtgZI GCGATG 1 cut(s) 2733
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 6 cut(s) 69, 192, 1480, 2120, 2461, 2855
BtuMI TCGCGA 1 cut(s) 2071
Cac8I GCNNGC 6 cut(s) 351, 505, 1598, 2025, 2689, 2910
CaiI CAGNNNCTG 1 cut(s) 871
CfoI GCGC 1 cut(s) 2677
Cfr13I GGNCC 4 cut(s) 651, 1421, 1493, 1796
ClaI ATCGAT 1 cut(s) 819
Csp6I GTAC 7 cut(s) 299, 564, 958, 1318, 1803, 1814, 2875
CspCI CAANNNNNGTGG 4 cut(s) 403, 438, 1543, 1578
CviQI GTAC 7 cut(s) 299, 564, 958, 1318, 1803, 1814, 2875
DinI GGCGCC 1 cut(s) 2676
DraI TTTAAA 1 cut(s) 2467
Eam1104I CTCTTC 5 cut(s) 154, 211, 514, 842, 1164
EarI CTCTTC 5 cut(s) 154, 211, 514, 842, 1164
Ecl136II GAGCTC 1 cut(s) 2219
Eco130I CCWWGG 5 cut(s) 1882, 2043, 2305, 2913, 2952
Eco147I AGGCCT 3 cut(s) 249, 2023, 2165
Eco24I GRGCYC 1 cut(s) 2221
Eco32I GATATC 1 cut(s) 720
Eco47I GGWCC 3 cut(s) 651, 1421, 1796
Eco53kI GAGCTC 1 cut(s) 2219
Eco57I CTGAAG 5 cut(s) 260, 269, 415, 848, 2960
Eco81I CCTNAGG 2 cut(s) 2089, 2699
Eco88I CYCGRG 1 cut(s) 400
Eco91I GGTNACC 2 cut(s) 623, 1010
EcoICRI GAGCTC 1 cut(s) 2219
EcoNI CCTNNNNNAGG 1 cut(s) 2309
EcoO65I GGTNACC 2 cut(s) 623, 1010
EcoRI GAATTC 1 cut(s) 1353
EcoRII CCWGG 2 cut(s) 152, 1429
EcoRV GATATC 1 cut(s) 720
EcoT14I CCWWGG 5 cut(s) 1882, 2043, 2305, 2913, 2952
EcoT22I ATGCAT 2 cut(s) 2008, 2855
EcoT38I GRGCYC 1 cut(s) 2221
EgeI GGCGCC 1 cut(s) 2676
EheI GGCGCC 1 cut(s) 2676
ErhI CCWWGG 5 cut(s) 1882, 2043, 2305, 2913, 2952
FalI AAGNNNNNCTT 2 cut(s) 2897, 2929
FaqI GGGAC 6 cut(s) 417, 787, 1075, 1206, 1311, 2505
FauNDI CATATG 1 cut(s) 1920
FblI GTMKAC 2 cut(s) 1083, 2199
Fnu4HI GCNGC 4 cut(s) 80, 1959, 2369, 2401
FokI GGATG 6 cut(s) 56, 179, 1487, 2107, 2468, 2862
FriOI GRGCYC 1 cut(s) 2221
Fsp4HI GCNGC 4 cut(s) 80, 1959, 2369, 2401
FspBI CTAG 9 cut(s) 407, 545, 561, 1367, 1649, 2330, 2477, 2684, 2846
GlaI GCGC 1 cut(s) 2676
GluI GCNGC 4 cut(s) 80, 1959, 2369, 2401
HaeII RGCGCY 1 cut(s) 2678
HaeIII GGCC 9 cut(s) 102, 249, 1494, 2023, 2087, 2165, 2770, 2912, 2947
HhaI GCGC 1 cut(s) 2677
Hin1I GRCGYC 1 cut(s) 2675
Hin6I GCGC 1 cut(s) 2675
HinP1I GCGC 1 cut(s) 2675
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 2 cut(s) 347, 2246
HphI GGTGA 9 cut(s) 341, 857, 929, 1022, 1773, 1907, 2433, 2716, 2809
Hpy166II GTNNAC 8 cut(s) 427, 479, 1084, 1684, 1781, 2200, 2441, 2922
Hpy8I GTNNAC 8 cut(s) 427, 479, 1084, 1684, 1781, 2200, 2441, 2922
Hpy99I CGWCG 4 cut(s) 65, 95, 170, 242
HpyCH4III ACNGT 7 cut(s) 395, 471, 614, 798, 858, 1390, 2197
HpyCH4IV ACGT 2 cut(s) 93, 2541
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 1946, 1955
HpySE526I ACGT 2 cut(s) 93, 2541
Hsp92I GRCGYC 1 cut(s) 2675
HspAI GCGC 1 cut(s) 2675
KasI GGCGCC 1 cut(s) 2674
LguI GCTCTTC 1 cut(s) 514
LmnI GCTCC 4 cut(s) 440, 584, 1134, 2216
Lsp1109I GCAGC 4 cut(s) 91, 1945, 2380, 2412
LweI GCATC 4 cut(s) 858, 2015, 2355, 2840
MaeI CTAG 9 cut(s) 407, 545, 561, 1367, 1649, 2330, 2477, 2684, 2846
MaeII ACGT 2 cut(s) 93, 2541
MaeIII GTNAC 8 cut(s) 623, 858, 1010, 1442, 1545, 2251, 2642, 2755
MfeI CAATTG 1 cut(s) 1469
MflI RGATCY 4 cut(s) 676, 1019, 1252, 1396
MhlI GDGCHC 5 cut(s) 99, 2060, 2221, 2904, 2924
Mly113I GGCGCC 1 cut(s) 2675
MmeI TCCRAC 9 cut(s) 144, 630, 794, 996, 1094, 1146, 1230, 1332, 1957
Mph1103I ATGCAT 2 cut(s) 2008, 2855
MroXI GAANNNNTTC 2 cut(s) 395, 1671
MseI TTAA 6 cut(s) 383, 1034, 1559, 1703, 2466, 2804
MslI CAYNNNNRTG 2 cut(s) 32, 74
MspA1I CMGCKG 1 cut(s) 1958
MspCI CTTAAG 1 cut(s) 382
MspR9I CCNGG 9 cut(s) 74, 154, 264, 629, 643, 700, 1215, 1431, 2126
MunI CAATTG 1 cut(s) 1469
Mva1269I GAATGC 1 cut(s) 2008
MvaI CCWGG 2 cut(s) 154, 1431
MvnI CGCG 1 cut(s) 2071
MwoI GCNNNNNNNGC 3 cut(s) 255, 1946, 1955
NarI GGCGCC 1 cut(s) 2675
NciI CCSGG 7 cut(s) 74, 264, 629, 643, 700, 1215, 2126
NcoI CCATGG 2 cut(s) 1882, 2952
NdeI CATATG 1 cut(s) 1920
NlaIV GGNNCC 4 cut(s) 761, 1797, 2321, 2676
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 6 cut(s) 858, 1010, 1442, 1545, 2251, 2755
NruI TCGCGA 1 cut(s) 2071
NsiI ATGCAT 2 cut(s) 2008, 2855
NspI RCATGY 2 cut(s) 2056, 2636
NspV TTCGAA 3 cut(s) 636, 1184, 1586
PceI AGGCCT 3 cut(s) 249, 2023, 2165
PciSI GCTCTTC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 165
PctI GAATGC 1 cut(s) 2008
PdmI GAANNNNTTC 2 cut(s) 395, 1671
PfeI GAWTC 9 cut(s) 280, 488, 638, 751, 1105, 1381, 1606, 2111, 2147
PflFI GACNNNGTC 1 cut(s) 798
PfoI TCCNGGA 2 cut(s) 152, 641
PkrI GCNGC 4 cut(s) 81, 1960, 2370, 2402
Ple19I CGATCG 1 cut(s) 2075
PluTI GGCGCC 1 cut(s) 2678
Psp124BI GAGCTC 1 cut(s) 2221
Psp6I CCWGG 2 cut(s) 152, 1429
PspEI GGTNACC 2 cut(s) 623, 1010
PspGI CCWGG 2 cut(s) 152, 1429
PspN4I GGNNCC 4 cut(s) 761, 1797, 2321, 2676
PspPI GGNCC 4 cut(s) 651, 1421, 1493, 1796
PstNI CAGNNNCTG 1 cut(s) 871
PsuI RGATCY 4 cut(s) 676, 1019, 1252, 1396
PsyI GACNNNGTC 1 cut(s) 798
PvuI CGATCG 1 cut(s) 2075
PvuII CAGCTG 1 cut(s) 1958
RruI TCGCGA 1 cut(s) 2071
RsaI GTAC 7 cut(s) 300, 565, 959, 1319, 1804, 1815, 2876
RsaNI GTAC 7 cut(s) 299, 564, 958, 1318, 1803, 1814, 2875
RseI CAYNNNNRTG 2 cut(s) 32, 74
SacI GAGCTC 1 cut(s) 2221
SapI GCTCTTC 1 cut(s) 514
SaqAI TTAA 6 cut(s) 383, 1034, 1559, 1703, 2466, 2804
SatI GCNGC 4 cut(s) 80, 1959, 2369, 2401
Sau96I GGNCC 4 cut(s) 651, 1421, 1493, 1796
ScaI AGTACT 2 cut(s) 565, 959
ScrFI CCNGG 9 cut(s) 74, 154, 264, 629, 643, 700, 1215, 1431, 2126
SduI GDGCHC 5 cut(s) 99, 2060, 2221, 2904, 2924
SfaNI GCATC 4 cut(s) 858, 2015, 2355, 2840
SfoI GGCGCC 1 cut(s) 2676
SfuI TTCGAA 3 cut(s) 636, 1184, 1586
SinI GGWCC 3 cut(s) 651, 1421, 1796
SmiMI CAYNNNNRTG 2 cut(s) 32, 74
SmlI CTYRAG 9 cut(s) 251, 293, 382, 943, 953, 1312, 1610, 2747, 2903
SmoI CTYRAG 9 cut(s) 251, 293, 382, 943, 953, 1312, 1610, 2747, 2903
SpeI ACTAGT 1 cut(s) 406
SseBI AGGCCT 3 cut(s) 249, 2023, 2165
SspDI GGCGCC 1 cut(s) 2674
SspMI CTAG 9 cut(s) 407, 545, 561, 1367, 1649, 2330, 2477, 2684, 2846
SstI GAGCTC 1 cut(s) 2221
StuI AGGCCT 3 cut(s) 249, 2023, 2165
StyD4I CCNGG 9 cut(s) 72, 152, 262, 627, 641, 698, 1213, 1429, 2124
StyI CCWWGG 5 cut(s) 1882, 2043, 2305, 2913, 2952
TaaI ACNGT 7 cut(s) 395, 471, 614, 798, 858, 1390, 2197
TaiI ACGT 2 cut(s) 96, 2544
TatI WGTACW 3 cut(s) 563, 957, 1802
TfiI GAWTC 9 cut(s) 280, 488, 638, 751, 1105, 1381, 1606, 2111, 2147
Tru1I TTAA 6 cut(s) 383, 1034, 1559, 1703, 2466, 2804
Tru9I TTAA 6 cut(s) 383, 1034, 1559, 1703, 2466, 2804
TseFI GTSAC 6 cut(s) 858, 1010, 1442, 1545, 2251, 2755
TseI GCWGC 4 cut(s) 79, 1958, 2368, 2400
Tsp45I GTSAC 6 cut(s) 858, 1010, 1442, 1545, 2251, 2755
TspDTI ATGAA 8 cut(s) 181, 1320, 1473, 1680, 1823, 2439, 2583, 2901
TspGWI ACGGA 1 cut(s) 1417
Tth111I GACNNNGTC 1 cut(s) 798
Vha464I CTTAAG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 2920
VpaK11BI GGWCC 3 cut(s) 651, 1421, 1796
XagI CCTNNNNNAGG 1 cut(s) 2309
XapI RAATTY 5 cut(s) 874, 1324, 1353, 1480, 2970
XceI RCATGY 2 cut(s) 2056, 2636
XcmI CCANNNNNNNNNTGG 3 cut(s) 1588, 1862, 2959
XmiI GTMKAC 2 cut(s) 1083, 2199
XmnI GAANNNNTTC 2 cut(s) 395, 1671
XspI CTAG 9 cut(s) 407, 545, 561, 1367, 1649, 2330, 2477, 2684, 2846
ZrmI AGTACT 2 cut(s) 565, 959
Zsp2I ATGCAT 2 cut(s) 2008, 2855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.