MD04G1027600.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016035
Physical Location & Seq
Forward (+)
3158664 .. 3162428
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1027600.v1.1.491

Sequence Viewer

Length: 3030 bp
ATGGCTTCTCTCGGATTCCTCCAACATATGCTGCCATCAATATTGATATTAGCATCTCTTCACGCCTGCATGGGAGACGCCACAGTTCCTTCACAGCTCAACAACGACGTTCTAGGCCTACTCGTCTTCAAATCCGACATCCATGATCCTTCCTCGTACCTCGCTTCATGGAATGAAGATGAGAACTCCCCTTGTTCTTGGGAATATGTACAATGCAATCCAGCCACCGGAAGAGTCTCTCAGCTGTCACTCGAAGGCCTAGGCCTAAAGGGAAAAATCGGAAAAGGGCTTCAGAACTTGCAAAATTTGAAGGTACTTTCTTTGTCAAGCAACAATTTCAGCGGAGACATTAGTCCGGAGAAGCTTGCTCTGCCTCCGAATCTCGAAAAGCTTAATTTAAGCCACAACAGTTTATCAGGATTAATTCCCACTAATCTTTTCAACATGAGCTCCATCAAATTTCTTGATCTTTCCGAGAACTCACTCTCAGGGCCACTCCCTGATAACCTGTTTGATAACTGCTTTTCGCTTCGCTATCTTTCGTTATCTGGTAACTTGCTTGAAGGTCCTTTGCCTAGCACACTGCCTAGATGCTCTTCTTTGAACAGTCTCAATCTCTCAAACAACCATTTCTCCGGTAACCCTGATTTCGCTTCTGGGATTTGGTCTTTGACAAGATTGCGCACTCTAGATCTTTCGAACAATGCCTTTTCCGGGTATGTGTCACAAGGGATATCAGCCGTACACAACTTGAAAGAGCTTCTGTTACAAAGCAATCACTTTTCGGGATCTTTGCCAGCGGATATAGGACTATGTCCTCACTTAGAGAAGATTGATTTGAGCTATAATATGTTCACTGATGCATTACAAGACTCACTTCAGAGGTTGAATTCTTTGACATTTTTCAGTCTGTCAGATAACATGTTCTCTGGTGATTTCCCTCAGTGGATTGGTAGCATGAGCAGCCTCAAATATTTGGATTTTTCGAACAATGGCTTCACCGGAAGCCTCCCACCTTCAATGAGTGACCTCAGATCACTCAATTATTTGAGTTTGTCAAACAACAAACTTGTTGGTACTATTCCAACTTCTCTTGCTTACTGCAATGATTTGTCGGTGATTCGGTTGAGGGGTAACACTTTCACTGGAAGCATACCAGAGGGCTTGTTTAATCTGGGGTTGGAGGAAATTGACTTCTCCCACATGGGACTTACAGGTTCTATTCCTCCGGGTTCCAGCAAACTCTTCGAATCTTTAAGGATGTTGGATTTGTCAAGCAACAATCTCAAAGGAAATATTCCGGCAGAAGTGGGACTTTTCTCCAACTTGAGGTACTTGAATTTGTCATGGAACAACCTTCAGTCAAGGATGCCTCCAGAGCTCGGCTTCTTTCAGAACTTAACGGTGCTAGATCTTCGAAACAGCGCCTTGTTCGGTTCTATCCCAGAGGATGTATGTGATTCAGCAAGCCTGGCAATTCTCCAATTGGATGGAAACTCGTTGACTGGCTCAATTCCTGATGAAATTGGCAACTGCTCATCTCTCTATTTGATGAGCTTGTCACATAACAATTTAAGTGGTACTATTCCGAAATCCATTTCGAAGCTAAATAAGCTCAAGATTCTTAAATTGGAGTTCAATGAGTTGAGTGGAGAGATACCCCAAGAACTTGGAAAACTAGAAAATCTACTTGCTGTGAACATATCATATAACAGGTTGGTAGGGAGGCTTCCTGCTGGGAGCGTATTTCAGAGCTTGGATCAAACTGCGCTGCAAGGAAATCTGGGAATTTGCTCACCATTGTTAAAGGGACCATGTACGATGAATGTGCCTAAGCCTCTTGTTCTAGATCCAACTGCGTACAACAACCAAATGGGCGGTCATAGACACCATAACAAATCTGCAACATCCACAAAAGACCAGCGCCGCACCTTCCTTAGCTTATCTGCCATTGTGGCAATTTCAGCAGCCAGCCTGATTGTTTTGGGAGTGATTGTTATTAGTCTTCTGAATGCATCCGCAAGGAGGAGGCCTGCATTTGTGGAAACTGCCTTGGAGAGCATGTGTTCAAGCTCTTCACGTTCAGGTAGTTTGGCATCGGGGAAGCTCATTCTCTTCGACTCAAGGTCATCCCCGGAATGGATTAGCAGTCCTGAATCCCTACTCAACAAGGCCTCAGAGATCGGCGAGGGAGTCTTTGGAACAGTTTACAAAATTCCACTTGGAGCTCAAGGAAGAGTAGTTGCTATCAAGAGGTTGGTCACATCAAATATAATCCAGTGCCTTGAAGATTTTGATAGAGAGGTTAGAATTCTGGGGAAAGCAAGACACCCGAATCTAATCGCTCTAAAAGGGTACTATTGGACTCCACAGATGCAGCTGTTGGTAACAGAGTTTGCCACCAATGGCAGCCTTCAATCCAAACTCCATGAAAGACCTCATTCAAGTCCACCTCTTTCATGGGCTAATAGGTTCAAAATCTTGCTTGGAACAGCAAAGGGACTTGCTCAATTGCACCACTCATACCGCCCGCCAATCATCCACTACAACATCAAACCTAGTAACATCTTGCTCGATGAGAGTTACAACGCGAAAATATCAGACTTTGCACTGGCAAGGCTCCTGACAAAGATAGACCAGCATGTAGTGAGTAACAGATTTCAGAGTGCACTAGGTTATGTGGCGCCGGAACTGGCATGCCAGAGCTTAAGGGTGAATGAAAAATGTGATGTGTATGGTTTCGGCGTGTTGATTCTTGAGATCGTGACTGGGAGGAGACCTGTGGAGTATGGGGAGGACAATGTGGTAATACTGAATGACCATGTTAGGGTTTTGCTTGAGCAAGGGAATGCGTTGGGGTGCATTGATGTTAGCATGGGGTATTACCCAGAGGATGAAGTCCTACCAGTGCTTAAGTTGGCTTTGGTTTGCACCTCACAGATTCCATCCTGCAGGCCTACCATGGCAGAAGTTGTTCAAATTATGCAGATTATCAAGACCCCAATTCCACAAACAATGGAGGCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1010

Amino Acids

110.12

Weight (kDa)

6.26

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 73 1.8e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 126 - 187 2.3e-08 Leucine rich repeat
LRR_8 PF13855 303 - 357 1.9e-07 Leucine rich repeat
LRR_14 PF23598 415 - 571 3.3e-16 Leucine-rich repeat region
Pkinase PF00069 724 - 993 3.6e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 724 - 993 2.4e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28040
fragaria_vesca FvH4_4g12350
malus_domestica MD04G1027600.v1.1
prunus_persica Prupe.1G177000_v2.0.a1
pyrus_communis pycom04g02420
rosa_chinensis RchiOBHm_Chr4g0410441
rosa_laevigata RLG00000008430
rosa_multiflora Rmu_sc0002111.1_g000005
rosa_roxburghii Rroxscaffold_5G00355250
rosa_rugosa Rorug04G0102700
rosa_samantha Rh4AG160700 Rh4BG159900 Rh4CG172800 Rh4DG155600
rosa_wichuraiana Rw4G013390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 2683
AccII CGCG 1 cut(s) 2591
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 7 cut(s) 342, 800, 1878, 1927, 2019, 2527, 2531
AclWI GGATC 4 cut(s) 140, 796, 1767, 1844
AcsI RAATTY 7 cut(s) 304, 458, 889, 1339, 1788, 2214, 2310
AcuI CTGAAG 3 cut(s) 275, 863, 1343
AcyI GRCGYC 2 cut(s) 78, 2684
AfiI CCNNNNNNNGG 7 cut(s) 227, 714, 1329, 1382, 2025, 2139, 2827
AflII CTTAAG 2 cut(s) 2707, 2912
AflIII ACRYGT 1 cut(s) 921
AhdI GACNNNNNGTC 1 cut(s) 2125
AjnI CCWGG 1 cut(s) 1470
Alw21I GWGCWC 4 cut(s) 452, 1383, 2230, 2671
Alw26I GTCTC 5 cut(s) 69, 241, 339, 614, 2770
Alw44I GTGCAC 1 cut(s) 2667
AlwI GGATC 4 cut(s) 140, 796, 1767, 1844
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 7 cut(s) 115, 256, 262, 491, 2030, 2172, 2954
ApaLI GTGCAC 1 cut(s) 2667
ApeKI GCWGC 6 cut(s) 31, 963, 1771, 1967, 2377, 2409
ApoI RAATTY 7 cut(s) 304, 458, 889, 1339, 1788, 2214, 2310
ArsI GACNNNNNNTTYG 2 cut(s) 1864, 1896
AseI ATTAAT 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 2973
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 5 cut(s) 684, 1427, 1771, 1926, 2686
AspS9I GGNCC 3 cut(s) 491, 566, 1811
AsuC2I CCSGG 3 cut(s) 715, 1230, 2135
AsuHPI GGTGA 5 cut(s) 944, 991, 1129, 1788, 2725
AsuII TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
AvaII GGWCC 2 cut(s) 566, 1811
AvrII CCTAGG 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 2671
BanI GGYRCC 1 cut(s) 2683
BanII GRGCYC 3 cut(s) 452, 1383, 2230
BarI GAAGNNNNNNTAC 2 cut(s) 1713, 1745
BbsI GAAGAC 2 cut(s) 118, 1997
Bbv12I GWGCWC 4 cut(s) 452, 1383, 2230, 2671
BbvI GCAGC 6 cut(s) 18, 975, 1758, 1979, 2389, 2421
BccI CCATC 4 cut(s) 43, 461, 1484, 2953
BceAI ACGGC 1 cut(s) 725
BcgI CGANNNNNNTGC 4 cut(s) 1228, 1262, 1810, 1844
BciT130I CCWGG 1 cut(s) 1472
BcnI CCSGG 3 cut(s) 715, 1230, 2135
BcoDI GTCTC 5 cut(s) 69, 241, 339, 614, 2770
BfmI CTRYAG 1 cut(s) 2950
BfoI RGCGCY 3 cut(s) 1428, 1927, 2687
BfrI CTTAAG 2 cut(s) 2707, 2912
BglI GCCNNNNNGGC 1 cut(s) 1955
BglII AGATCT 2 cut(s) 691, 1411
BisI GCNGC 7 cut(s) 32, 964, 1772, 1927, 1968, 2378, 2410
BlnI CCTAGG 1 cut(s) 259
BlsI GCNGC 7 cut(s) 33, 965, 1773, 1928, 1969, 2379, 2411
Bme1390I CCNGG 4 cut(s) 715, 1230, 1472, 2135
Bme18I GGWCC 2 cut(s) 566, 1811
BmeRI GACNNNNNGTC 1 cut(s) 2125
BmgT120I GGNCC 3 cut(s) 491, 566, 1811
BmiI GGNNCC 4 cut(s) 1234, 1812, 2621, 2685
BmrFI CCNGG 4 cut(s) 715, 1230, 1472, 2135
BmrI ACTGGG 1 cut(s) 2778
BmsI GCATC 7 cut(s) 62, 581, 850, 1359, 2024, 2105, 2364
BmuI ACTGGG 1 cut(s) 2778
BoxI GACNNNNGTC 1 cut(s) 351
BpiI GAAGAC 2 cut(s) 118, 1997
BpmI CTGGAG 1 cut(s) 1359
Bpu10I CCTNAGC 2 cut(s) 1833, 1937
Bpu14I TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
BpuEI CTTGAG 6 cut(s) 1348, 1601, 2107, 2214, 2777, 2858
BpuMI CCSGG 3 cut(s) 715, 1230, 2135
BsaHI GRCGYC 2 cut(s) 78, 2684
BsaI GGTCTC 1 cut(s) 2770
BsaJI CCNNGG 4 cut(s) 259, 2052, 2133, 2961
BsaWI WCCGGW 4 cut(s) 227, 355, 635, 1001
BsaXI ACNNNNNCTCC 8 cut(s) 434, 464, 617, 647, 1980, 2010, 2048, 2078
Bsc4I CCNNNNNNNGG 7 cut(s) 227, 714, 1329, 1382, 2025, 2139, 2827
Bse1I ACTGG 7 cut(s) 1150, 1510, 2278, 2616, 2697, 2773, 2906
Bse3DI GCAATG 1 cut(s) 1111
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 1 cut(s) 1472
BseDI CCNNGG 4 cut(s) 259, 2052, 2133, 2961
BseLI CCNNNNNNNGG 7 cut(s) 227, 714, 1329, 1382, 2025, 2139, 2827
BseMI GCAATG 1 cut(s) 1111
BseMII CTCAG 5 cut(s) 254, 501, 956, 1045, 2190
BseNI ACTGG 7 cut(s) 1150, 1510, 2278, 2616, 2697, 2773, 2906
BseRI GAGGAG 2 cut(s) 2041, 2788
BseSI GKGCMC 1 cut(s) 2671
BseXI GCAGC 6 cut(s) 18, 975, 1758, 1979, 2389, 2421
BseYI CCCAGC 1 cut(s) 1736
Bsh1236I CGCG 1 cut(s) 2591
BshFI GGCC 7 cut(s) 117, 258, 264, 493, 2032, 2174, 2956
BshNI GGYRCC 1 cut(s) 2683
BsiHKAI GWGCWC 4 cut(s) 452, 1383, 2230, 2671
BsiSI CCGG 9 cut(s) 228, 356, 636, 714, 1002, 1229, 1301, 2135, 2687
BslFI GGGAC 4 cut(s) 1221, 1326, 1824, 2514
BslI CCNNNNNNNGG 7 cut(s) 227, 714, 1329, 1382, 2025, 2139, 2827
BsmAI GTCTC 5 cut(s) 69, 241, 339, 614, 2770
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 4 cut(s) 1221, 1326, 1824, 2514
BsmI GAATGC 2 cut(s) 2017, 2854
BsnI GGCC 7 cut(s) 117, 258, 264, 493, 2032, 2174, 2956
Bso31I GGTCTC 1 cut(s) 2770
Bsp119I TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
Bsp1286I GDGCHC 4 cut(s) 452, 1383, 2230, 2671
Bsp13I TCCGGA 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 2961
BspACI CCGC 7 cut(s) 342, 800, 1878, 1927, 2019, 2527, 2531
BspANI GGCC 7 cut(s) 117, 258, 264, 493, 2032, 2174, 2956
BspCNI CTCAG 5 cut(s) 253, 500, 955, 1044, 2189
BspEI TCCGGA 1 cut(s) 355
BspFNI CGCG 1 cut(s) 2591
BspLI GGNNCC 4 cut(s) 1234, 1812, 2621, 2685
BspMAI CTGCAG 1 cut(s) 2954
BspPI GGATC 4 cut(s) 140, 796, 1767, 1844
BspQI GCTCTTC 2 cut(s) 601, 2080
BspT104I TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
BspT107I GGYRCC 1 cut(s) 2683
BspTI CTTAAG 2 cut(s) 2707, 2912
BspTNI GGTCTC 1 cut(s) 2770
BsrDI GCAATG 1 cut(s) 1111
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 7 cut(s) 1150, 1510, 2278, 2616, 2697, 2773, 2906
BssECI CCNNGG 4 cut(s) 259, 2052, 2133, 2961
BssNI GRCGYC 2 cut(s) 78, 2684
BssT1I CCWWGG 3 cut(s) 259, 2052, 2961
Bst2UI CCWGG 1 cut(s) 1472
Bst4CI ACNGT 5 cut(s) 85, 410, 608, 1405, 2206
Bst6I CTCTTC 7 cut(s) 63, 226, 601, 1250, 2080, 2120, 2230
BstACI GRCGYC 2 cut(s) 78, 2684
BstAFI CTTAAG 2 cut(s) 2707, 2912
BstAUI TGTACA 1 cut(s) 208
BstBI TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
BstC8I GCNNGC 9 cut(s) 67, 366, 798, 1468, 1972, 2034, 2531, 2698, 2954
BstDEI CTNAG 8 cut(s) 240, 487, 823, 942, 1031, 1833, 1937, 2176
BstDSI CCRYGG 1 cut(s) 2961
BstEII GGTNACC 1 cut(s) 638
BstFNI CGCG 1 cut(s) 2591
BstH2I RGCGCY 3 cut(s) 1428, 1927, 2687
BstHHI GCGC 5 cut(s) 684, 1427, 1771, 1926, 2686
BstMAI GTCTC 5 cut(s) 69, 241, 339, 614, 2770
BstMWI GCNNNNNNNGC 7 cut(s) 370, 963, 1378, 1472, 1612, 1955, 1964
BstNI CCWGG 1 cut(s) 1472
BstNSI RCATGY 4 cut(s) 925, 2065, 2645, 2700
BstPAI GACNNNNGTC 1 cut(s) 351
BstPI GGTNACC 1 cut(s) 638
BstSCI CCNGG 4 cut(s) 713, 1228, 1470, 2133
BstSFI CTRYAG 1 cut(s) 2950
BstSLI GKGCMC 1 cut(s) 2671
BstUI CGCG 1 cut(s) 2591
BstV1I GCAGC 6 cut(s) 18, 975, 1758, 1979, 2389, 2421
BstV2I GAAGAC 2 cut(s) 118, 1997
BstX2I RGATCY 4 cut(s) 691, 788, 1411, 1849
BstXI CCANNNNNNTGG 2 cut(s) 1490, 1670
BstYI RGATCY 4 cut(s) 691, 788, 1411, 1849
BsuRI GGCC 7 cut(s) 117, 258, 264, 493, 2032, 2174, 2956
BtgI CCRYGG 1 cut(s) 2961
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 7 cut(s) 581, 855, 950, 1143, 2285, 2609, 2913
Cac8I GCNNGC 9 cut(s) 67, 366, 798, 1468, 1972, 2034, 2531, 2698, 2954
CfoI GCGC 5 cut(s) 684, 1427, 1771, 1926, 2686
Cfr13I GGNCC 3 cut(s) 491, 566, 1811
CseI GACGC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 1558, 1593
DdeI CTNAG 8 cut(s) 240, 487, 823, 942, 1031, 1833, 1937, 2176
DinI GGCGCC 1 cut(s) 2685
DriI GACNNNNNGTC 1 cut(s) 2125
Eam1104I CTCTTC 7 cut(s) 63, 226, 601, 1250, 2080, 2120, 2230
Eam1105I GACNNNNNGTC 1 cut(s) 2125
EarI CTCTTC 7 cut(s) 63, 226, 601, 1250, 2080, 2120, 2230
Ecl136II GAGCTC 3 cut(s) 450, 1381, 2228
Eco130I CCWWGG 3 cut(s) 259, 2052, 2961
Eco147I AGGCCT 6 cut(s) 117, 258, 264, 2032, 2174, 2956
Eco24I GRGCYC 3 cut(s) 452, 1383, 2230
Eco31I GGTCTC 1 cut(s) 2770
Eco32I GATATC 1 cut(s) 735
Eco47I GGWCC 2 cut(s) 566, 1811
Eco53kI GAGCTC 3 cut(s) 450, 1381, 2228
Eco57I CTGAAG 3 cut(s) 275, 863, 1343
Eco91I GGTNACC 1 cut(s) 638
EcoICRI GAGCTC 3 cut(s) 450, 1381, 2228
EcoO109I RGGNCCY 1 cut(s) 566
EcoO65I GGTNACC 1 cut(s) 638
EcoRI GAATTC 2 cut(s) 889, 2310
EcoRII CCWGG 1 cut(s) 1470
EcoRV GATATC 1 cut(s) 735
EcoT14I CCWWGG 3 cut(s) 259, 2052, 2961
EcoT22I ATGCAT 2 cut(s) 865, 2017
EcoT38I GRGCYC 3 cut(s) 452, 1383, 2230
EgeI GGCGCC 1 cut(s) 2685
EheI GGCGCC 1 cut(s) 2685
ErhI CCWWGG 3 cut(s) 259, 2052, 2961
Esp3I CGTCTC 1 cut(s) 69
FalI AAGNNNNNCTT 4 cut(s) 861, 893, 1299, 1331
FaqI GGGAC 4 cut(s) 1221, 1326, 1824, 2514
FauI CCCGC 1 cut(s) 2538
FauNDI CATATG 1 cut(s) 27
Fnu4HI GCNGC 7 cut(s) 32, 964, 1772, 1927, 1968, 2378, 2410
FriOI GRGCYC 3 cut(s) 452, 1383, 2230
Fsp4HI GCNGC 7 cut(s) 32, 964, 1772, 1927, 1968, 2378, 2410
FspI TGCGCA 1 cut(s) 683
GlaI GCGC 5 cut(s) 683, 1426, 1770, 1925, 2685
GluI GCNGC 7 cut(s) 32, 964, 1772, 1927, 1968, 2378, 2410
GsaI CCCAGC 1 cut(s) 1740
GsuI CTGGAG 1 cut(s) 1359
HaeII RGCGCY 3 cut(s) 1428, 1927, 2687
HaeIII GGCC 7 cut(s) 117, 258, 264, 493, 2032, 2174, 2956
HapII CCGG 9 cut(s) 228, 356, 636, 714, 1002, 1229, 1301, 2135, 2687
HgaI GACGC 1 cut(s) 86
HhaI GCGC 5 cut(s) 684, 1427, 1771, 1926, 2686
Hin1I GRCGYC 2 cut(s) 78, 2684
Hin6I GCGC 5 cut(s) 682, 1425, 1769, 1924, 2684
HinP1I GCGC 5 cut(s) 682, 1425, 1769, 1924, 2684
HincII GTYRAC 1 cut(s) 1503
HindII GTYRAC 1 cut(s) 1503
HindIII AAGCTT 2 cut(s) 362, 389
HpaII CCGG 9 cut(s) 228, 356, 636, 714, 1002, 1229, 1301, 2135, 2687
HphI GGTGA 5 cut(s) 944, 991, 1129, 1788, 2725
Hpy166II GTNNAC 7 cut(s) 745, 855, 1503, 1699, 2209, 2450, 2669
Hpy8I GTNNAC 7 cut(s) 745, 855, 1503, 1699, 2209, 2450, 2669
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 9 cut(s) 99, 159, 248, 304, 557, 1026, 1367, 1942, 2423
HpyCH4III ACNGT 5 cut(s) 85, 410, 608, 1405, 2206
HpyCH4IV ACGT 2 cut(s) 108, 2080
HpyF10VI GCNNNNNNNGC 7 cut(s) 370, 963, 1378, 1472, 1612, 1955, 1964
HpyF3I CTNAG 8 cut(s) 240, 487, 823, 942, 1031, 1833, 1937, 2176
HpySE526I ACGT 2 cut(s) 108, 2080
Hsp92I GRCGYC 2 cut(s) 78, 2684
HspAI GCGC 5 cut(s) 682, 1425, 1769, 1924, 2684
KasI GGCGCC 1 cut(s) 2683
Kpn2I TCCGGA 1 cut(s) 355
LguI GCTCTTC 2 cut(s) 601, 2080
LmnI GCTCC 4 cut(s) 455, 1740, 2225, 2625
Lsp1109I GCAGC 6 cut(s) 18, 975, 1758, 1979, 2389, 2421
LweI GCATC 7 cut(s) 62, 581, 850, 1359, 2024, 2105, 2364
MaeII ACGT 2 cut(s) 108, 2080
MfeI CAATTG 2 cut(s) 1484, 2510
MflI RGATCY 4 cut(s) 691, 788, 1411, 1849
MhlI GDGCHC 4 cut(s) 452, 1383, 2230, 2671
Mly113I GGCGCC 1 cut(s) 2684
MlyI GAGTC 5 cut(s) 243, 866, 2114, 2202, 2359
MmeI TCCRAC 7 cut(s) 46, 159, 1109, 1161, 1245, 1347, 1877
Mph1103I ATGCAT 2 cut(s) 865, 2017
MroI TCCGGA 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 2973
MspA1I CMGCKG 4 cut(s) 244, 342, 800, 2380
MspCI CTTAAG 2 cut(s) 2707, 2912
MspI CCGG 9 cut(s) 228, 356, 636, 714, 1002, 1229, 1301, 2135, 2687
MspR9I CCNGG 4 cut(s) 715, 1230, 1472, 2135
MunI CAATTG 2 cut(s) 1484, 2510
Mva1269I GAATGC 2 cut(s) 2017, 2854
MvaI CCWGG 1 cut(s) 1472
MvnI CGCG 1 cut(s) 2591
MwoI GCNNNNNNNGC 7 cut(s) 370, 963, 1378, 1472, 1612, 1955, 1964
NarI GGCGCC 1 cut(s) 2684
NciI CCSGG 3 cut(s) 715, 1230, 2135
NcoI CCATGG 1 cut(s) 2961
NdeI CATATG 1 cut(s) 27
NlaIV GGNNCC 4 cut(s) 1234, 1812, 2621, 2685
NmeAIII GCCGAG 1 cut(s) 1362
NmuCI GTSAC 6 cut(s) 246, 723, 1025, 1560, 2260, 2764
NsbI TGCGCA 1 cut(s) 683
NsiI ATGCAT 2 cut(s) 865, 2017
NspI RCATGY 4 cut(s) 925, 2065, 2645, 2700
NspV TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
PaeI GCATGC 1 cut(s) 2700
PceI AGGCCT 6 cut(s) 117, 258, 264, 2032, 2174, 2956
PciI ACATGT 1 cut(s) 921
PciSI GCTCTTC 2 cut(s) 601, 2080
PctI GAATGC 2 cut(s) 2017, 2854
PdmI GAANNNNTTC 1 cut(s) 2973
PflFI GACNNNGTC 1 cut(s) 813
PkrI GCNGC 7 cut(s) 33, 965, 1773, 1928, 1969, 2379, 2411
PleI GAGTC 5 cut(s) 242, 866, 2114, 2201, 2359
PluTI GGCGCC 1 cut(s) 2687
PpsI GAGTC 5 cut(s) 242, 866, 2114, 2201, 2359
PpuMI RGGWCCY 1 cut(s) 566
PscI ACATGT 1 cut(s) 921
PshAI GACNNNNGTC 1 cut(s) 351
PshBI ATTAAT 1 cut(s) 422
Psp124BI GAGCTC 3 cut(s) 452, 1383, 2230
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 1470
PspEI GGTNACC 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 1736
PspGI CCWGG 1 cut(s) 1470
PspN4I GGNNCC 4 cut(s) 1234, 1812, 2621, 2685
PspPI GGNCC 3 cut(s) 491, 566, 1811
PspPPI RGGWCCY 1 cut(s) 566
PstI CTGCAG 1 cut(s) 2954
PsuI RGATCY 4 cut(s) 691, 788, 1411, 1849
PsyI GACNNNGTC 1 cut(s) 813
PvuII CAGCTG 2 cut(s) 244, 2380
SacI GAGCTC 3 cut(s) 452, 1383, 2230
SapI GCTCTTC 2 cut(s) 601, 2080
SatI GCNGC 7 cut(s) 32, 964, 1772, 1927, 1968, 2378, 2410
Sau96I GGNCC 3 cut(s) 491, 566, 1811
SbfI CCTGCAGG 1 cut(s) 2954
SchI GAGTC 5 cut(s) 243, 866, 2114, 2202, 2359
ScrFI CCNGG 4 cut(s) 715, 1230, 1472, 2135
SdaI CCTGCAGG 1 cut(s) 2954
SduI GDGCHC 4 cut(s) 452, 1383, 2230, 2671
SfaNI GCATC 7 cut(s) 62, 581, 850, 1359, 2024, 2105, 2364
SfcI CTRYAG 1 cut(s) 2950
SfoI GGCGCC 1 cut(s) 2685
SfuI TTCGAA 5 cut(s) 698, 986, 1248, 1417, 1601
SinI GGWCC 2 cut(s) 566, 1811
SmlI CTYRAG 8 cut(s) 1327, 1616, 2122, 2229, 2707, 2756, 2837, 2912
SmoI CTYRAG 8 cut(s) 1327, 1616, 2122, 2229, 2707, 2756, 2837, 2912
SphI GCATGC 1 cut(s) 2700
Sse8387I CCTGCAGG 1 cut(s) 2954
SseBI AGGCCT 6 cut(s) 117, 258, 264, 2032, 2174, 2956
SsiI CCGC 7 cut(s) 342, 800, 1878, 1927, 2019, 2527, 2531
SspDI GGCGCC 1 cut(s) 2683
SspI AATATT 3 cut(s) 42, 974, 1297
SstI GAGCTC 3 cut(s) 452, 1383, 2230
StuI AGGCCT 6 cut(s) 117, 258, 264, 2032, 2174, 2956
StyD4I CCNGG 4 cut(s) 713, 1228, 1470, 2133
StyI CCWWGG 3 cut(s) 259, 2052, 2961
TaaI ACNGT 5 cut(s) 85, 410, 608, 1405, 2206
TaiI ACGT 2 cut(s) 111, 2083
TaqI TCGA 9 cut(s) 252, 384, 698, 986, 1248, 1417, 1601, 2118, 2574
TatI WGTACW 1 cut(s) 208
TauI GCSGC 1 cut(s) 1929
TscAI CASTG 7 cut(s) 588, 862, 950, 1150, 2285, 2616, 2913
TseFI GTSAC 6 cut(s) 246, 723, 1025, 1560, 2260, 2764
TseI GCWGC 6 cut(s) 31, 963, 1771, 1967, 2377, 2409
Tsp45I GTSAC 6 cut(s) 246, 723, 1025, 1560, 2260, 2764
TspDTI ATGAA 8 cut(s) 156, 189, 1536, 1838, 2445, 2448, 2733, 2910
TspRI CASTG 7 cut(s) 588, 862, 950, 1150, 2285, 2616, 2913
Tth111I GACNNNGTC 1 cut(s) 813
Vha464I CTTAAG 2 cut(s) 2707, 2912
VneI GTGCAC 1 cut(s) 2667
VpaK11BI GGWCC 2 cut(s) 566, 1811
VspI ATTAAT 1 cut(s) 422
XapI RAATTY 7 cut(s) 304, 458, 889, 1339, 1788, 2214, 2310
XbaI TCTAGA 2 cut(s) 688, 1846
XceI RCATGY 4 cut(s) 925, 2065, 2645, 2700
XcmI CCANNNNNNNNNTGG 1 cut(s) 2457
XmaJI CCTAGG 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 2973
Zsp2I ATGCAT 2 cut(s) 865, 2017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.