Rh4DG155600
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016035
Physical Location & Seq
Reverse (-)
31078209 .. 31081852
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG155600.1

Sequence Viewer

Length: 3033 bp
ATGGGTTTTCTCAGATTCCTCATATACACGCTGGCAATAACGTCCATGGTTTCTCTGCAACGCTGCATGGGCGACGACCCCATCCCGGCACAGCTAAACTACGACGTTTTGGGCCTCCTCGTCTTCAAATCCGACCTCCACGACCCTTCTTCCTACCTCGGTTCCTGGAACGAAGACGACGACTCCCCATGTTCATGGAACTTCATCCAATGCAACCCTACCACCGGAAGAGTCTCTCACCTCTCCCTCGACGGCTTAGGCCTTGAGGGCAAAATCGGAAAAGGTCTTCAGAATCTTCAGCACCTCAAGGTACTCTCTTTATCCAACAACAACTTCACTGGTGAGCTTAGTCCAGAAAAACTTGCTCTTCCTCCAAATCTCCAAACACTCAACTTAAGCCGAAACAGTTTCTCGGGACTAGTTCCCACTACTCTTGTGAACTTCAGCTCCATCAGATTTCTTGATCTTTCTGAGAACTCACTCTCTGGTTCACTCCCTGATTCGCTCTTTGATGCTTGTTTTTCGCTTCGCTACCTTTCTTTATCTGGAAACTTGCTAGAAGGGAACTTGCCTAGTACTCTTTCCAGATGCTCTTCTTTGAATGGCCTCAACATCTCCAACAACCGCTTCTCCGGTAACCCGGATTTCGAATCCGGGATTTGGTCCTTGGAACGGCTTCGTTCTTTGGATCTTTCAAACAATGCTCTTTCCGGGTTTGTGCCAAAAGGGATATCAGCCACACATAATTTGAAGGAGCTTTTAATCCAGAGGAACCACTTTTCTGGGCCAATGCCTTTTGATATTGGACTGTGTCCGCACTTGGGTTGGATTGATTTCAGTGAAAATCTGTTCACTGGTGAGTTACCGCAGTCACTTCAGAAGTTGAATTCCTTGACATTTATGAGTCTTTCAGATAACATGTTCAATGGTGATTTTCCTCAATGGATTGGGAACATGAGCAGCCTACAGTACTTGGACTTGTCCAACAATGGCTTCACGGGAAGGCTCCCACCATCAATGGGTGACCTTACATCTCTCAGTTATCTAAGTCTGTCTAACAACAAACTTGTTGGGACACTTCCAACTTCTCTTGTCTACTGCAACAAGTTGTCTGTGATTCGGTTGAGGGGTAATGATTTCAGTGGGAGCATACCAGAAGGTTTGTTTGGTCTGGGCTTGGAAGAGATAGATTTTTCGGATATGGGACTTACAGGTTCAATTCCACCCTGTTCGAGTAAATTGTTTGAGTCTTTGAAGATGTTGGATCTATCAAGGAACAATCTCAAAGGCAACATTCCAGCAGAGGTGGGACTTCTATCCAACTTGAGATACTTGAATCTTTCATGGAACAATCTTCAATCAAGAATTCCCCCAGAGCTTGGATTCTTTTCGAACCTAACCGTGCTAGATCTCCGCAATAGCGCATTGTATGGTTCAATCCCAGGAGATATATGTGATTCTGGGAGTTTGGATATTCTCCAATTGGATGGTAATTCATTGACTGGCCCAATTCCTGATGAGATTGGGAATTGCTCATCCCTCTATTTGATGAGTTTGTCACATAACAATTTAAGTGGTGCTATTCCAAAATCCATTTCGAAGTTGAGCAAGCTTGAGATTCTCAAGTTAGAGTTCAATCAGTTGGGTGGAGAGATACCGCAAGAGCTTGGAAAATTGGAAAATCTTCTTGCTGTGAACATTTCATACAACAGATTAACAGGGAGGCTTCCTGTAGGGAGTGTGTTTCAGAGCTTGGATCAAAGTGCTTTGCAAGGAAACTTGGGCATTTGTTCACCATTGTTGAAGGGACCATGTACAATGAATGTACCTAAGCCTCTTGTTCTTGATCCAAATGCCTACCCCAACCAAATGGGTGGTGGGGATCATAGATTCCAAGGCGAATCTTCAGGGTCCCGAAAAGGACATCACCATATGTTCCTTAGCATATCTGCTATTGTGGCAATTTCAGCTGCTACATTGATCGCTGTTGGAGTAATTGTCATTACCCTTCTAAATGCATCTGCAAGAAGGAGGCCTGCATTTGTGGAAACTGCCTTGGAAAGCATGTGTTCGAGCTCTTCGCGATCGGGTAGTTTGGCATCAGGGAAGCTCATTCTGTTTGACTCAAGGTCATCGCCGGATTGGATCAGCAGTCCTGAATCACTACTAAACAAGGCCTCTGAGCTCGGGGAGGGAGTCTTTGGGACAGTCTACAAAGTTCCACTGGGAGCTCAGGGAAGAATGGTGGCAATCAAAAAGCTTGTGACAACAAACATACTCCAATGTCTTGAAGACTTTGACAGAGAAGTTCGAGTCCTTGGAAAGGCGAGGCACCCGAATCTAGTAGCCCTGAAAGGATATTACTGGACTCCACAGATGCAGCTACTTGTAAATGAGTATGCCCCCAATGGCAACCTACAATCCAAACTACATGATAGGCTTCATTCAAGTTCACCTCTTCCATGGGATGACAGGTTCAAAATCCTGCTTGGAATAGCAAGGGGACTTTCCCATTTGCACCATTCTTTCCGCCCGCCCATCATTCACTACAACATAAAACCAAGTAACATATTGCTCGATGAAAACCTCAACCCGAAGATTTCTGATTTTGCACTCGCAAGGCTACTAACCAAGATCGACAGGCACGTAGTGAGTAACAGATTTCAGAGTGCATTGGGCTATGTGGCACCTGAACTAGCTTGCCAGAGCCTAAGGGTAAACGAGAAATGCGATGTGTATGGTTTCGGTGTCTTGATCCTTGAGCTTGTCACTGGTAGAAGGCCAGTGGAATATGGGGAAGACAATGTGGTGATATTGACTGATCATGTGAGGGTTTTGCTTGAACAAGGCAATGTGTTGGGATGCATTGATCATAGCATGGGGGAGTACCCAGAGGATGAAGTCCTACCAGTGTTCAAGTTGGCCTTGGTGTGCACTTCTCAGATACCATCTTGCAGGCCAACCATGGCAGAAGTGGTGCAAATTCTTCAGATTATCAAGACTCCACTTCCACAGAGAATTGAGCGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1010

Amino Acids

110.6

Weight (kDa)

6.06

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 72 5.3e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 101 - 161 2.5e-08 Leucine rich repeat
LRR_8 PF13855 313 - 356 1.9e-06 Leucine rich repeat
LRR_8 PF13855 321 - 380 1.9e-06 Leucine rich repeat
LRR_4 PF12799 321 - 360 6e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 413 - 570 2.6e-14 Leucine-rich repeat region
Pkinase PF00069 725 - 994 2.3e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 725 - 995 5.3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28040
fragaria_vesca FvH4_4g12350
malus_domestica MD04G1027600.v1.1
prunus_persica Prupe.1G177000_v2.0.a1
pyrus_communis pycom04g02420
rosa_chinensis RchiOBHm_Chr4g0410441
rosa_laevigata RLG00000008430
rosa_multiflora Rmu_sc0002111.1_g000005
rosa_roxburghii Rroxscaffold_5G00355250
rosa_rugosa Rorug04G0102700
rosa_samantha Rh4AG160700 Rh4BG159900 Rh4CG172800 Rh4DG155600
rosa_wichuraiana Rw4G013390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2331, 2686
AccB7I CCANNNNNTGG 1 cut(s) 1379
AccI GTMKAC 2 cut(s) 1095, 2211
AccII CGCG 1 cut(s) 2083
AciI CCGC 7 cut(s) 625, 815, 866, 1414, 1658, 2530, 2534
AclWI GGATC 7 cut(s) 696, 1272, 1764, 1841, 1890, 2153, 2749
AcsI RAATTY 3 cut(s) 886, 1365, 2982
AcuI CTGAAG 6 cut(s) 272, 281, 427, 860, 1890, 2972
AdeI CACNNNGTG 1 cut(s) 2650
AfaI GTAC 6 cut(s) 312, 577, 971, 1816, 1827, 2888
AfiI CCNNNNNNNGG 8 cut(s) 85, 224, 660, 672, 821, 1019, 1379, 2323
AflII CTTAAG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 918
AhdI GACNNNNNGTC 1 cut(s) 2128
AhlI ACTAGT 1 cut(s) 418
AjnI CCWGG 2 cut(s) 164, 1441
AloI GAACNNNNNNTCC 4 cut(s) 431, 463, 2459, 2491
Alw21I GWGCWC 4 cut(s) 2078, 2187, 2233, 2936
Alw26I GTCTC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 2932
AlwI GGATC 7 cut(s) 696, 1272, 1764, 1841, 1890, 2153, 2749
Ama87I CYCGRG 2 cut(s) 412, 2186
ApaLI GTGCAC 1 cut(s) 2932
ApeKI GCWGC 4 cut(s) 63, 960, 1970, 2380
ApoI RAATTY 3 cut(s) 886, 1365, 2982
Asp700I GAANNNNTTC 2 cut(s) 675, 1683
AspLEI GCGC 1 cut(s) 1424
AspS9I GGNCC 6 cut(s) 112, 663, 785, 1505, 1808, 1911
AsuC2I CCSGG 4 cut(s) 86, 641, 655, 712
AsuHPI GGTGA 9 cut(s) 230, 353, 869, 941, 1034, 1785, 1919, 2445, 2821
AsuII TTCGAA 3 cut(s) 648, 1391, 1598
AvaI CYCGRG 2 cut(s) 412, 2186
AvaII GGWCC 3 cut(s) 663, 1808, 1911
AxyI CCTNAGG 1 cut(s) 2711
BaeGI GKGCMC 1 cut(s) 2936
BanI GGYRCC 2 cut(s) 2331, 2686
BanII GRGCYC 3 cut(s) 2078, 2187, 2233
BbsI GAAGAC 5 cut(s) 115, 180, 278, 2298, 2805
Bbv12I GWGCWC 4 cut(s) 2078, 2187, 2233, 2936
BbvI GCAGC 4 cut(s) 50, 972, 1957, 2392
BccI CCATC 6 cut(s) 89, 458, 1021, 1481, 2546, 2956
BceAI ACGGC 2 cut(s) 268, 689
BciT130I CCWGG 2 cut(s) 166, 1443
BclI TGATCA 2 cut(s) 2821, 2869
BcnI CCSGG 4 cut(s) 86, 641, 655, 712
BcoDI GTCTC 1 cut(s) 238
BcuI ACTAGT 1 cut(s) 418
BfaI CTAG 6 cut(s) 419, 557, 573, 1406, 2342, 2696
BfmI CTRYAG 2 cut(s) 965, 1731
BfrI CTTAAG 1 cut(s) 394
BglI GCCNNNNNGGC 1 cut(s) 267
BglII AGATCT 1 cut(s) 1408
BisI GCNGC 4 cut(s) 64, 961, 1971, 2381
BlsI GCNGC 4 cut(s) 65, 962, 1972, 2382
BmcAI AGTACT 2 cut(s) 577, 971
Bme1390I CCNGG 6 cut(s) 86, 166, 641, 655, 712, 1443
Bme18I GGWCC 3 cut(s) 663, 1808, 1911
BmeRI GACNNNNNGTC 1 cut(s) 2128
BmeT110I CYCGRG 2 cut(s) 412, 2186
BmgT120I GGNCC 6 cut(s) 112, 663, 785, 1505, 1808, 1911
BmiI GGNNCC 8 cut(s) 163, 773, 1007, 1809, 1912, 1913, 2333, 2688
BmrFI CCNGG 6 cut(s) 86, 166, 641, 655, 712, 1443
BmrI ACTGGG 1 cut(s) 2234
BmsI GCATC 6 cut(s) 502, 578, 2027, 2108, 2367, 2852
BmuI ACTGGG 1 cut(s) 2234
BpiI GAAGAC 5 cut(s) 115, 180, 278, 2298, 2805
Bpu10I CCTNAGC 4 cut(s) 256, 1830, 1940, 2232
Bpu14I TTCGAA 3 cut(s) 648, 1391, 1598
BpuEI CTTGAG 7 cut(s) 284, 290, 1345, 1607, 1634, 2110, 2780
BpuMI CCSGG 4 cut(s) 86, 641, 655, 712
BsaAI YACGTR 1 cut(s) 2647
BsaWI WCCGGW 2 cut(s) 224, 632
BsaXI ACNNNNNCTCC 8 cut(s) 167, 197, 431, 461, 614, 644, 1983, 2013
Bsc4I CCNNNNNNNGG 8 cut(s) 85, 224, 660, 672, 821, 1019, 1379, 2323
Bse1I ACTGG 8 cut(s) 343, 859, 1507, 2229, 2369, 2776, 2783, 2909
Bse21I CCTNAGG 1 cut(s) 2711
Bse3DI GCAATG 1 cut(s) 2857
BseBI CCWGG 2 cut(s) 166, 1443
BseGI GGATG 7 cut(s) 81, 204, 1492, 1535, 2473, 2867, 2902
BseLI CCNNNNNNNGG 8 cut(s) 85, 224, 660, 672, 821, 1019, 1379, 2323
BseMI GCAATG 1 cut(s) 2857
BseMII CTCAG 6 cut(s) 25, 462, 1051, 2172, 2246, 2954
BseNI ACTGG 8 cut(s) 343, 859, 1507, 2229, 2369, 2776, 2783, 2909
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 2936
BseXI GCAGC 4 cut(s) 50, 972, 1957, 2392
Bsh1236I CGCG 1 cut(s) 2083
Bsh1285I CGRYCG 1 cut(s) 2087
BshNI GGYRCC 2 cut(s) 2331, 2686
BsiEI CGRYCG 1 cut(s) 2087
BsiHKAI GWGCWC 4 cut(s) 2078, 2187, 2233, 2936
BsiHKCI CYCGRG 2 cut(s) 412, 2186
BsiSI CCGG 7 cut(s) 86, 225, 633, 641, 654, 711, 2138
BslFI GGGAC 8 cut(s) 429, 1087, 1218, 1323, 1821, 1897, 2218, 2517
BslI CCNNNNNNNGG 8 cut(s) 85, 224, 660, 672, 821, 1019, 1379, 2323
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 8 cut(s) 429, 1087, 1218, 1323, 1821, 1897, 2218, 2517
BsoBI CYCGRG 2 cut(s) 412, 2186
Bsp119I TTCGAA 3 cut(s) 648, 1391, 1598
Bsp1286I GDGCHC 4 cut(s) 2078, 2187, 2233, 2936
Bsp1407I TGTACA 1 cut(s) 1814
Bsp19I CCATGG 3 cut(s) 45, 2462, 2964
Bsp68I TCGCGA 1 cut(s) 2083
BspACI CCGC 7 cut(s) 625, 815, 866, 1414, 1658, 2530, 2534
BspCNI CTCAG 6 cut(s) 24, 463, 1050, 2173, 2245, 2953
BspFNI CGCG 1 cut(s) 2083
BspLI GGNNCC 8 cut(s) 163, 773, 1007, 1809, 1912, 1913, 2333, 2688
BspPI GGATC 7 cut(s) 696, 1272, 1764, 1841, 1890, 2153, 2749
BspQI GCTCTTC 3 cut(s) 372, 598, 2083
BspT104I TTCGAA 3 cut(s) 648, 1391, 1598
BspT107I GGYRCC 2 cut(s) 2331, 2686
BspTI CTTAAG 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 2857
BsrGI TGTACA 1 cut(s) 1814
BsrI ACTGG 8 cut(s) 343, 859, 1507, 2229, 2369, 2776, 2783, 2909
BssT1I CCWWGG 8 cut(s) 45, 666, 1894, 2055, 2317, 2462, 2925, 2964
Bst2UI CCWGG 2 cut(s) 166, 1443
Bst4CI ACNGT 5 cut(s) 407, 810, 969, 1402, 2209
Bst6I CTCTTC 6 cut(s) 223, 372, 598, 1176, 2083, 2463
BstAFI CTTAAG 1 cut(s) 394
BstAUI TGTACA 1 cut(s) 1814
BstBAI YACGTR 1 cut(s) 2647
BstBI TTCGAA 3 cut(s) 648, 1391, 1598
BstC8I GCNNGC 6 cut(s) 33, 1610, 2037, 2534, 2701, 2957
BstDSI CCRYGG 3 cut(s) 45, 2462, 2964
BstEII GGTNACC 2 cut(s) 635, 1022
BstENI CCTNNNNNAGG 1 cut(s) 2321
BstF5I GGATG 7 cut(s) 81, 204, 1492, 1535, 2473, 2867, 2902
BstFNI CGCG 1 cut(s) 2083
BstHHI GCGC 1 cut(s) 1424
BstMAI GTCTC 1 cut(s) 238
BstMCI CGRYCG 1 cut(s) 2087
BstMWI GCNNNNNNNGC 4 cut(s) 69, 267, 1958, 1967
BstNI CCWGG 2 cut(s) 166, 1443
BstNSI RCATGY 2 cut(s) 922, 2068
BstPI GGTNACC 2 cut(s) 635, 1022
BstSCI CCNGG 6 cut(s) 84, 164, 639, 653, 710, 1441
BstSFI CTRYAG 2 cut(s) 965, 1731
BstSLI GKGCMC 1 cut(s) 2936
BstUI CGCG 1 cut(s) 2083
BstV1I GCAGC 4 cut(s) 50, 972, 1957, 2392
BstV2I GAAGAC 5 cut(s) 115, 180, 278, 2298, 2805
BstX2I RGATCY 3 cut(s) 688, 1264, 1408
BstXI CCANNNNNNTGG 5 cut(s) 195, 782, 1487, 1870, 1874
BstYI RGATCY 3 cut(s) 688, 1264, 1408
Bsu36I CCTNAGG 1 cut(s) 2711
BtgI CCRYGG 3 cut(s) 45, 2462, 2964
BtgZI GCGATG 2 cut(s) 2118, 2745
BtsCI GGATG 7 cut(s) 81, 204, 1492, 1535, 2473, 2867, 2902
BtsIMutI CAGTG 8 cut(s) 336, 844, 852, 1147, 2222, 2769, 2790, 2916
BtuMI TCGCGA 1 cut(s) 2083
Cac8I GCNNGC 6 cut(s) 33, 1610, 2037, 2534, 2701, 2957
CfoI GCGC 1 cut(s) 1424
Cfr13I GGNCC 6 cut(s) 112, 663, 785, 1505, 1808, 1911
Csp6I GTAC 6 cut(s) 311, 576, 970, 1815, 1826, 2887
CspCI CAANNNNNGTGG 4 cut(s) 415, 450, 1555, 1590
CviQI GTAC 6 cut(s) 311, 576, 970, 1815, 1826, 2887
DraIII CACNNNGTG 1 cut(s) 2650
DriI GACNNNNNGTC 1 cut(s) 2128
Eam1104I CTCTTC 6 cut(s) 223, 372, 598, 1176, 2083, 2463
Eam1105I GACNNNNNGTC 1 cut(s) 2128
EarI CTCTTC 6 cut(s) 223, 372, 598, 1176, 2083, 2463
EciI GGCGGA 1 cut(s) 2519
Ecl136II GAGCTC 3 cut(s) 2076, 2185, 2231
Eco130I CCWWGG 8 cut(s) 45, 666, 1894, 2055, 2317, 2462, 2925, 2964
Eco147I AGGCCT 3 cut(s) 261, 2035, 2177
Eco24I GRGCYC 3 cut(s) 2078, 2187, 2233
Eco32I GATATC 1 cut(s) 732
Eco47I GGWCC 3 cut(s) 663, 1808, 1911
Eco53kI GAGCTC 3 cut(s) 2076, 2185, 2231
Eco57I CTGAAG 6 cut(s) 272, 281, 427, 860, 1890, 2972
Eco81I CCTNAGG 1 cut(s) 2711
Eco88I CYCGRG 2 cut(s) 412, 2186
Eco91I GGTNACC 2 cut(s) 635, 1022
EcoICRI GAGCTC 3 cut(s) 2076, 2185, 2231
EcoNI CCTNNNNNAGG 1 cut(s) 2321
EcoO109I RGGNCCY 1 cut(s) 1911
EcoO65I GGTNACC 2 cut(s) 635, 1022
EcoRI GAATTC 2 cut(s) 886, 1365
EcoRII CCWGG 2 cut(s) 164, 1441
EcoRV GATATC 1 cut(s) 732
EcoT14I CCWWGG 8 cut(s) 45, 666, 1894, 2055, 2317, 2462, 2925, 2964
EcoT22I ATGCAT 2 cut(s) 2020, 2867
EcoT38I GRGCYC 3 cut(s) 2078, 2187, 2233
ErhI CCWWGG 8 cut(s) 45, 666, 1894, 2055, 2317, 2462, 2925, 2964
FalI AAGNNNNNCTT 2 cut(s) 2909, 2941
FaqI GGGAC 8 cut(s) 429, 1087, 1218, 1323, 1821, 1897, 2218, 2517
FauI CCCGC 1 cut(s) 2541
FauNDI CATATG 1 cut(s) 1932
FbaI TGATCA 2 cut(s) 2821, 2869
FblI GTMKAC 2 cut(s) 1095, 2211
Fnu4HI GCNGC 4 cut(s) 64, 961, 1971, 2381
FokI GGATG 7 cut(s) 68, 191, 1499, 1522, 2480, 2874, 2909
FriOI GRGCYC 3 cut(s) 2078, 2187, 2233
Fsp4HI GCNGC 4 cut(s) 64, 961, 1971, 2381
FspBI CTAG 6 cut(s) 419, 557, 573, 1406, 2342, 2696
GlaI GCGC 1 cut(s) 1423
GluI GCNGC 4 cut(s) 64, 961, 1971, 2381
HapII CCGG 7 cut(s) 86, 225, 633, 641, 654, 711, 2138
HhaI GCGC 1 cut(s) 1424
Hin6I GCGC 1 cut(s) 1422
HinP1I GCGC 1 cut(s) 1422
HindIII AAGCTT 2 cut(s) 1610, 2258
HpaII CCGG 7 cut(s) 86, 225, 633, 641, 654, 711, 2138
HphI GGTGA 9 cut(s) 230, 353, 869, 941, 1034, 1785, 1919, 2445, 2821
Hpy99I CGWCG 4 cut(s) 77, 107, 182, 254
HpyAV CCTTC 9 cut(s) 156, 554, 745, 996, 1151, 1798, 2018, 2022, 2772
HpyCH4III ACNGT 5 cut(s) 407, 810, 969, 1402, 2209
HpyCH4IV ACGT 3 cut(s) 41, 105, 2646
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 267, 1958, 1967
HpySE526I ACGT 3 cut(s) 41, 105, 2646
HspAI GCGC 1 cut(s) 1422
KflI GGGWCCC 1 cut(s) 1911
Ksp22I TGATCA 2 cut(s) 2821, 2869
LguI GCTCTTC 3 cut(s) 372, 598, 2083
LmnI GCTCC 5 cut(s) 452, 754, 1011, 1146, 2228
Lsp1109I GCAGC 4 cut(s) 50, 972, 1957, 2392
LweI GCATC 6 cut(s) 502, 578, 2027, 2108, 2367, 2852
MaeI CTAG 6 cut(s) 419, 557, 573, 1406, 2342, 2696
MaeII ACGT 3 cut(s) 41, 105, 2646
MaeIII GTNAC 9 cut(s) 635, 861, 870, 1022, 1557, 2263, 2564, 2654, 2767
MfeI CAATTG 1 cut(s) 1481
MflI RGATCY 3 cut(s) 688, 1264, 1408
MhlI GDGCHC 4 cut(s) 2078, 2187, 2233, 2936
MlyI GAGTC 9 cut(s) 176, 240, 913, 1256, 2117, 2205, 2322, 2362, 2995
MmeI TCCRAC 9 cut(s) 156, 348, 642, 806, 1008, 1106, 1242, 1344, 1969
Mph1103I ATGCAT 2 cut(s) 2020, 2867
MroXI GAANNNNTTC 2 cut(s) 675, 1683
MseI TTAA 4 cut(s) 395, 761, 1571, 1715
MslI CAYNNNNRTG 1 cut(s) 193
MspA1I CMGCKG 1 cut(s) 1970
MspCI CTTAAG 1 cut(s) 394
MspI CCGG 7 cut(s) 86, 225, 633, 641, 654, 711, 2138
MspR9I CCNGG 6 cut(s) 86, 166, 641, 655, 712, 1443
MunI CAATTG 1 cut(s) 1481
MvaI CCWGG 2 cut(s) 166, 1443
MvnI CGCG 1 cut(s) 2083
MwoI GCNNNNNNNGC 4 cut(s) 69, 267, 1958, 1967
NciI CCSGG 4 cut(s) 86, 641, 655, 712
NcoI CCATGG 3 cut(s) 45, 2462, 2964
NdeI CATATG 1 cut(s) 1932
NlaIV GGNNCC 8 cut(s) 163, 773, 1007, 1809, 1912, 1913, 2333, 2688
NmuCI GTSAC 5 cut(s) 870, 1022, 1557, 2263, 2767
NruI TCGCGA 1 cut(s) 2083
NsiI ATGCAT 2 cut(s) 2020, 2867
NspI RCATGY 2 cut(s) 922, 2068
NspV TTCGAA 3 cut(s) 648, 1391, 1598
PceI AGGCCT 3 cut(s) 261, 2035, 2177
PciI ACATGT 1 cut(s) 918
PciSI GCTCTTC 3 cut(s) 372, 598, 2083
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 2643, 2727
PdmI GAANNNNTTC 2 cut(s) 675, 1683
PflFI GACNNNGTC 1 cut(s) 810
PflMI CCANNNNNTGG 1 cut(s) 1379
PfoI TCCNGGA 2 cut(s) 164, 653
PkrI GCNGC 4 cut(s) 65, 962, 1972, 2382
Ple19I CGATCG 1 cut(s) 2087
PleI GAGTC 9 cut(s) 176, 239, 912, 1255, 2117, 2204, 2321, 2362, 2995
PpsI GAGTC 9 cut(s) 176, 239, 912, 1255, 2117, 2204, 2321, 2362, 2995
Ppu21I YACGTR 1 cut(s) 2647
PpuMI RGGWCCY 1 cut(s) 1911
PscI ACATGT 1 cut(s) 918
Psp124BI GAGCTC 3 cut(s) 2078, 2187, 2233
Psp5II RGGWCCY 1 cut(s) 1911
Psp6I CCWGG 2 cut(s) 164, 1441
PspEI GGTNACC 2 cut(s) 635, 1022
PspGI CCWGG 2 cut(s) 164, 1441
PspN4I GGNNCC 8 cut(s) 163, 773, 1007, 1809, 1912, 1913, 2333, 2688
PspPI GGNCC 6 cut(s) 112, 663, 785, 1505, 1808, 1911
PspPPI RGGWCCY 1 cut(s) 1911
PsrI GAACNNNNNNTAC 2 cut(s) 1688, 1720
PsuI RGATCY 3 cut(s) 688, 1264, 1408
PsyI GACNNNGTC 1 cut(s) 810
PvuI CGATCG 1 cut(s) 2087
PvuII CAGCTG 1 cut(s) 1970
RruI TCGCGA 1 cut(s) 2083
RsaI GTAC 6 cut(s) 312, 577, 971, 1816, 1827, 2888
RsaNI GTAC 6 cut(s) 311, 576, 970, 1815, 1826, 2887
RseI CAYNNNNRTG 1 cut(s) 193
SacI GAGCTC 3 cut(s) 2078, 2187, 2233
SapI GCTCTTC 3 cut(s) 372, 598, 2083
SaqAI TTAA 4 cut(s) 395, 761, 1571, 1715
SatI GCNGC 4 cut(s) 64, 961, 1971, 2381
Sau96I GGNCC 6 cut(s) 112, 663, 785, 1505, 1808, 1911
ScaI AGTACT 2 cut(s) 577, 971
SchI GAGTC 9 cut(s) 176, 240, 913, 1256, 2117, 2205, 2322, 2362, 2995
ScrFI CCNGG 6 cut(s) 86, 166, 641, 655, 712, 1443
SduI GDGCHC 4 cut(s) 2078, 2187, 2233, 2936
SfaNI GCATC 6 cut(s) 502, 578, 2027, 2108, 2367, 2852
SfcI CTRYAG 2 cut(s) 965, 1731
SfuI TTCGAA 3 cut(s) 648, 1391, 1598
SinI GGWCC 3 cut(s) 663, 1808, 1911
SmiMI CAYNNNNRTG 1 cut(s) 193
SmlI CTYRAG 8 cut(s) 263, 305, 394, 1324, 1613, 1622, 2125, 2759
SmoI CTYRAG 8 cut(s) 263, 305, 394, 1324, 1613, 1622, 2125, 2759
SpeI ACTAGT 1 cut(s) 418
SseBI AGGCCT 3 cut(s) 261, 2035, 2177
SsiI CCGC 7 cut(s) 625, 815, 866, 1414, 1658, 2530, 2534
SspMI CTAG 6 cut(s) 419, 557, 573, 1406, 2342, 2696
SstI GAGCTC 3 cut(s) 2078, 2187, 2233
StuI AGGCCT 3 cut(s) 261, 2035, 2177
StyD4I CCNGG 6 cut(s) 84, 164, 639, 653, 710, 1441
StyI CCWWGG 8 cut(s) 45, 666, 1894, 2055, 2317, 2462, 2925, 2964
TaaI ACNGT 5 cut(s) 407, 810, 969, 1402, 2209
TaiI ACGT 3 cut(s) 44, 108, 2649
TaqI TCGA 9 cut(s) 249, 648, 1232, 1391, 1598, 2072, 2311, 2577, 2637
TatI WGTACW 3 cut(s) 575, 969, 1814
Tru1I TTAA 4 cut(s) 395, 761, 1571, 1715
Tru9I TTAA 4 cut(s) 395, 761, 1571, 1715
TscAI CASTG 8 cut(s) 343, 844, 859, 1147, 2229, 2776, 2790, 2916
TseFI GTSAC 5 cut(s) 870, 1022, 1557, 2263, 2767
TseI GCWGC 4 cut(s) 63, 960, 1970, 2380
Tsp45I GTSAC 5 cut(s) 870, 1022, 1557, 2263, 2767
TspDTI ATGAA 9 cut(s) 183, 193, 1332, 1485, 1692, 1835, 2432, 2595, 2913
TspRI CASTG 8 cut(s) 343, 844, 859, 1147, 2229, 2776, 2790, 2916
Tth111I GACNNNGTC 1 cut(s) 810
Van91I CCANNNNNTGG 1 cut(s) 1379
Vha464I CTTAAG 1 cut(s) 394
VneI GTGCAC 1 cut(s) 2932
VpaK11BI GGWCC 3 cut(s) 663, 1808, 1911
XagI CCTNNNNNAGG 1 cut(s) 2321
XapI RAATTY 3 cut(s) 886, 1365, 2982
XceI RCATGY 2 cut(s) 922, 2068
XcmI CCANNNNNNNNNTGG 2 cut(s) 1874, 2971
XmiI GTMKAC 2 cut(s) 1095, 2211
XmnI GAANNNNTTC 2 cut(s) 675, 1683
XspI CTAG 6 cut(s) 419, 557, 573, 1406, 2342, 2696
ZrmI AGTACT 2 cut(s) 577, 971
Zsp2I ATGCAT 2 cut(s) 2020, 2867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.