FvH4_4g13580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017648
Physical Location & Seq
Reverse (-)
17154708 .. 17157083
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13580.t1

Sequence Viewer

Length: 486 bp
ATGGCAACCCCTCGCTCACTCTGCCGTACCCTCCGCCCCTTCTCCACCACCACCAGACCCTCTCACCGCCATTTCTCCGATCCCACCTCCTTAATTGGGAGCCGCGTCCCCCAGGACAAGGCAAAGCTAGCAGAGCTGCGGAGATATTACAACCTACTGGCGCTGCGCAGAGACTACGTGAAGCATGTGAAGGAGGTCCGCATCAAGTCCCTCATGGAGGTTGAGCTCATGAGGCTCGAGATGCTCCGCGTCGACGAGGCCCGTCAAGAAGCCCTGAACGAGCAGACCAGGAGGCTCAAGGCTGAGGCCGCTCGGCTTCGCGCCCAAAAGCGGGAAGCCGCCAAGCAGCAGCAGGAACAAACCCTCTTGAAACAGAAAGCTGAAAAACTTGAGAATTTGAAGAAGAAGGGAATGCTAAGGGAAGAGAAGAAAAAGGAGAAGAATCAACTGCTGCGCCAGAGTTCCTCTGGGATCAAGGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.99

Weight (kDa)

10.47

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017648)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13580
rosa_chinensis RchiOBHm_Chr4g0414331
rosa_laevigata RLG00000008157
rosa_multiflora Rmu_sc0005196.1_g000009 Rmu_sc0041188.1_g000001
rosa_roxburghii Rroxscaffold_5G00358200
rosa_rugosa Rorug04G0126900
rosa_samantha Rh4AG185000 Rh4BG184600 Rh4CG197900 Rh4DG182200
rosa_wichuraiana Rw4G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 252
AccII CGCG 3 cut(s) 105, 249, 321
AciI CCGC 9 cut(s) 34, 67, 103, 139, 199, 247, 309, 331, 339
AclWI GGATC 2 cut(s) 74, 479
AcsI RAATTY 1 cut(s) 394
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 5 cut(s) 96, 118, 217, 330, 331
AgsI TTSAA 2 cut(s) 370, 400
AjnI CCWGG 2 cut(s) 111, 287
AluBI AGCT 4 cut(s) 127, 136, 226, 380
AluI AGCT 4 cut(s) 127, 136, 226, 380
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 74, 479
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 2 cut(s) 258, 306
ApeKI GCWGC 5 cut(s) 136, 163, 346, 349, 451
ApoI RAATTY 1 cut(s) 394
AspLEI GCGC 4 cut(s) 163, 168, 323, 456
AspS9I GGNCC 2 cut(s) 196, 259
AsuHPI GGTGA 1 cut(s) 56
AsuNHI GCTAGC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 303
BbvI GCAGC 5 cut(s) 123, 150, 358, 361, 438
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 113, 289
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 8 cut(s) 103, 137, 164, 309, 339, 347, 350, 452
BlsI GCNGC 8 cut(s) 104, 138, 165, 310, 340, 348, 351, 453
Bme1390I CCNGG 2 cut(s) 113, 289
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 196, 259
BmiI GGNNCC 2 cut(s) 101, 480
BmrFI CCNGG 2 cut(s) 113, 289
BmsI GCATC 2 cut(s) 210, 231
BmtI GCTAGC 1 cut(s) 131
Bpu10I CCTNAGC 2 cut(s) 303, 416
BpuEI CTTGAG 2 cut(s) 281, 410
BsaAI YACGTR 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 118, 217, 330, 331
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 2 cut(s) 113, 289
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 5 cut(s) 96, 118, 217, 330, 331
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 5 cut(s) 123, 150, 358, 361, 438
Bsh1236I CGCG 3 cut(s) 105, 249, 321
BshFI GGCC 2 cut(s) 260, 308
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 236
BslFI GGGAC 2 cut(s) 92, 193
BslI CCNNNNNNNGG 5 cut(s) 96, 118, 217, 330, 331
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 92, 193
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 2 cut(s) 260, 308
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 2 cut(s) 79, 471
BspACI CCGC 9 cut(s) 34, 67, 103, 139, 199, 247, 309, 331, 339
BspANI GGCC 2 cut(s) 260, 308
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 3 cut(s) 105, 249, 321
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 101, 480
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 74, 479
BsrBI CCGCTC 1 cut(s) 311
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 2 cut(s) 79, 471
Bst2UI CCWGG 2 cut(s) 113, 289
Bst6I CTCTTC 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 303, 416
BstENI CCTNNNNNAGG 1 cut(s) 215
BstFNI CGCG 3 cut(s) 105, 249, 321
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 4 cut(s) 163, 168, 323, 456
BstKTI GATC 2 cut(s) 82, 474
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 79, 471
BstMWI GCNNNNNNNGC 6 cut(s) 21, 128, 133, 232, 241, 308
BstNI CCWGG 2 cut(s) 113, 289
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 111, 287
BstUI CGCG 3 cut(s) 105, 249, 321
BstV1I GCAGC 5 cut(s) 123, 150, 358, 361, 438
BsuRI GGCC 2 cut(s) 260, 308
Cac8I GCNNGC 1 cut(s) 129
CciI TCATGA 1 cut(s) 228
CfoI GCGC 4 cut(s) 163, 168, 323, 456
Cfr13I GGNCC 2 cut(s) 196, 259
CseI GACGC 2 cut(s) 94, 238
Csp6I GTAC 1 cut(s) 27
CviAII CATG 3 cut(s) 185, 214, 229
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 2 cut(s) 303, 416
DpnI GATC 2 cut(s) 81, 473
DpnII GATC 2 cut(s) 79, 471
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 111, 287
EcoT38I GRGCYC 1 cut(s) 228
FaeI CATG 3 cut(s) 188, 217, 232
FaiI YATR 4 cut(s) 186, 215, 230, 484
FaqI GGGAC 2 cut(s) 92, 193
FatI CATG 3 cut(s) 184, 213, 228
FauI CCCGC 1 cut(s) 324
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 8 cut(s) 103, 137, 164, 309, 339, 347, 350, 452
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 8 cut(s) 103, 137, 164, 309, 339, 347, 350, 452
FspBI CTAG 1 cut(s) 128
FspI TGCGCA 1 cut(s) 167
GlaI GCGC 4 cut(s) 162, 167, 322, 455
GluI GCNGC 8 cut(s) 103, 137, 164, 309, 339, 347, 350, 452
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 260, 308
HgaI GACGC 2 cut(s) 94, 238
HhaI GCGC 4 cut(s) 163, 168, 323, 456
Hin1II CATG 3 cut(s) 188, 217, 232
Hin6I GCGC 4 cut(s) 161, 166, 321, 454
HinP1I GCGC 4 cut(s) 161, 166, 321, 454
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 442
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 4 cut(s) 229, 238, 266, 367
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 2 cut(s) 254, 257
HpyAV CCTTC 3 cut(s) 49, 184, 400
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 128, 133, 232, 241, 308
HpyF3I CTNAG 2 cut(s) 303, 416
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 3 cut(s) 188, 217, 232
HspAI GCGC 4 cut(s) 161, 166, 321, 454
Kzo9I GATC 2 cut(s) 79, 471
LmnI GCTCC 2 cut(s) 99, 249
Lsp1109I GCAGC 5 cut(s) 123, 150, 358, 361, 438
LweI GCATC 2 cut(s) 210, 231
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 177
MalI GATC 2 cut(s) 81, 473
MbiI CCGCTC 1 cut(s) 311
MboI GATC 2 cut(s) 79, 471
MboII GAAGA 5 cut(s) 412, 415, 434, 439, 451
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 93, 394
MseI TTAA 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 113, 289
MteI GCGCNGCGC 1 cut(s) 164
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 2 cut(s) 113, 289
MvnI CGCG 3 cut(s) 105, 249, 321
MwoI GCNNNNNNNGC 6 cut(s) 21, 128, 133, 232, 241, 308
NdeII GATC 2 cut(s) 79, 471
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 3 cut(s) 188, 217, 232
NlaIV GGNNCC 2 cut(s) 101, 480
NmeAIII GCCGAG 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 167
NspI RCATGY 1 cut(s) 188
PaeR7I CTCGAG 1 cut(s) 236
PagI TCATGA 1 cut(s) 228
PctI GAATGC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 442
PkrI GCNGC 8 cut(s) 104, 138, 165, 310, 340, 348, 351, 453
Ppu21I YACGTR 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 111, 287
PspGI CCWGG 2 cut(s) 111, 287
PspN4I GGNNCC 2 cut(s) 101, 480
PspPI GGNCC 2 cut(s) 196, 259
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 8 cut(s) 103, 137, 164, 309, 339, 347, 350, 452
Sau3AI GATC 2 cut(s) 79, 471
Sau96I GGNCC 2 cut(s) 196, 259
ScrFI CCNGG 2 cut(s) 113, 289
SduI GDGCHC 1 cut(s) 228
SetI ASST 9 cut(s) 89, 129, 138, 156, 180, 198, 222, 228, 382
SfaNI GCATC 2 cut(s) 210, 231
Sfr274I CTCGAG 1 cut(s) 236
SgrDI CGTCGACG 1 cut(s) 251
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 236
SmlI CTYRAG 3 cut(s) 236, 296, 389
SmoI CTYRAG 3 cut(s) 236, 296, 389
Sse9I AATT 2 cut(s) 93, 394
SsiI CCGC 9 cut(s) 34, 67, 103, 139, 199, 247, 309, 331, 339
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 111, 287
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 237, 252
TasI AATT 2 cut(s) 93, 394
TauI GCSGC 3 cut(s) 105, 311, 341
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TseI GCWGC 5 cut(s) 136, 163, 346, 349, 451
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 1 cut(s) 215
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.