Rh4AG185000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017648
Physical Location & Seq
Reverse (-)
46016380 .. 46018899
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG185000.1

Sequence Viewer

Length: 375 bp
ATGGCGACCCCTCGCTCACTCTACCGCACCCTCCGCCCCTTCTCCACAACCACTAGACCCTCTCGTCATTTCTCCGATCCCAAAGAGGAGGCGAAGCTAGCAGCGCTGCGCAGGTACTACAATCTACTGGTGCTGCGCAGAGACTACGCGAAGCATGTGAAGGAGGTCCGCGTCAAGTCCATCATGGAGGTGGAGCTCATGAGGCTCGAGATGCTCCGCGTCGACGAGGCCCGTAAGGAAGCCCAGAATGAGCAGACCAGGAGGCTCAAGGCTGAGGCCGCCAGGGTTCGCGCCCAAAAGTGGGAGGCTGCCAAGCACGAGACGGAACAAGCACTAGTATGTCATTTGTTTCCTCTCTGTTTTCTCTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

14.84

Weight (kDa)

10.0

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017648)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13580
rosa_chinensis RchiOBHm_Chr4g0414331
rosa_laevigata RLG00000008157
rosa_multiflora Rmu_sc0005196.1_g000009 Rmu_sc0041188.1_g000001
rosa_roxburghii Rroxscaffold_5G00358200
rosa_rugosa Rorug04G0126900
rosa_samantha Rh4AG185000 Rh4BG184600 Rh4CG197900 Rh4DG182200
rosa_wichuraiana Rw4G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 63
Acc16I TGCGCA 2 cut(s) 110, 137
Acc36I ACCTGC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 222
AccII CGCG 4 cut(s) 149, 171, 219, 291
AciI CCGC 5 cut(s) 25, 34, 169, 217, 279
AclWI GGATC 1 cut(s) 71
AfaI GTAC 1 cut(s) 116
AfeI AGCGCT 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 300, 301
AhlI ACTAGT 1 cut(s) 334
AjnI CCWGG 2 cut(s) 257, 281
AluBI AGCT 2 cut(s) 97, 196
AluI AGCT 2 cut(s) 97, 196
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 135, 314
AlwI GGATC 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 206
Aor51HI AGCGCT 1 cut(s) 105
AoxI GGCC 2 cut(s) 228, 276
ApeKI GCWGC 4 cut(s) 101, 106, 133, 308
AspLEI GCGC 4 cut(s) 106, 111, 138, 293
AspS9I GGNCC 2 cut(s) 166, 229
AsuNHI GCTAGC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 166
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 4 cut(s) 93, 113, 120, 295
BccI CCATC 1 cut(s) 188
BciT130I CCWGG 2 cut(s) 259, 283
BcoDI GTCTC 2 cut(s) 135, 314
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 3 cut(s) 54, 98, 335
BfoI RGCGCY 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 102
BisI GCNGC 5 cut(s) 102, 107, 134, 279, 309
BlsI GCNGC 5 cut(s) 103, 108, 135, 280, 310
Bme1390I CCNGG 2 cut(s) 259, 283
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 166, 229
BmrFI CCNGG 2 cut(s) 259, 283
BmsI GCATC 1 cut(s) 201
BmtI GCTAGC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 300, 301
Bse1I ACTGG 1 cut(s) 132
BseBI CCWGG 2 cut(s) 259, 283
BseDI CCNNGG 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 300, 301
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 4 cut(s) 93, 113, 120, 295
Bsh1236I CGCG 4 cut(s) 149, 171, 219, 291
BshFI GGCC 2 cut(s) 230, 278
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 300, 301
BsmAI GTCTC 2 cut(s) 135, 314
BsmBI CGTCTC 1 cut(s) 314
BsnI GGCC 2 cut(s) 230, 278
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 5 cut(s) 25, 34, 169, 217, 279
BspANI GGCC 2 cut(s) 230, 278
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 4 cut(s) 149, 171, 219, 291
BspHI TCATGA 1 cut(s) 198
BspMI ACCTGC 1 cut(s) 102
BspOI GCTAGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 2 cut(s) 259, 283
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 273
BstFNI CGCG 4 cut(s) 149, 171, 219, 291
BstH2I RGCGCY 1 cut(s) 107
BstHHI GCGC 4 cut(s) 106, 111, 138, 293
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 135, 314
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 6 cut(s) 33, 98, 103, 202, 211, 278
BstNI CCWGG 2 cut(s) 259, 283
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 257, 281
BstUI CGCG 4 cut(s) 149, 171, 219, 291
BstV1I GCAGC 4 cut(s) 93, 113, 120, 295
BsuRI GGCC 2 cut(s) 230, 278
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 99
CciI TCATGA 1 cut(s) 198
CfoI GCGC 4 cut(s) 106, 111, 138, 293
Cfr13I GGNCC 2 cut(s) 166, 229
CseI GACGC 2 cut(s) 160, 208
Csp6I GTAC 1 cut(s) 115
CviAII CATG 3 cut(s) 155, 184, 199
CviJI RGCY 9 cut(s) 97, 196, 205, 230, 242, 265, 272, 278, 308
CviKI_1 RGCY 9 cut(s) 97, 196, 205, 230, 242, 265, 272, 278, 308
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
DrdI GACNNNNNNGTC 1 cut(s) 63
DseDI GACNNNNNNGTC 1 cut(s) 63
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 166
Eco47III AGCGCT 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 257, 281
EcoT38I GRGCYC 1 cut(s) 198
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 3 cut(s) 158, 187, 202
FaiI YATR 4 cut(s) 156, 185, 200, 340
FatI CATG 3 cut(s) 154, 183, 198
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 5 cut(s) 102, 107, 134, 279, 309
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 5 cut(s) 102, 107, 134, 279, 309
FspBI CTAG 3 cut(s) 54, 98, 335
FspI TGCGCA 2 cut(s) 110, 137
GlaI GCGC 4 cut(s) 105, 110, 137, 292
GluI GCNGC 5 cut(s) 102, 107, 134, 279, 309
HaeII RGCGCY 1 cut(s) 107
HaeIII GGCC 2 cut(s) 230, 278
HgaI GACGC 2 cut(s) 160, 208
HhaI GCGC 4 cut(s) 106, 111, 138, 293
Hin1II CATG 3 cut(s) 158, 187, 202
Hin6I GCGC 4 cut(s) 104, 109, 136, 291
HinP1I GCGC 4 cut(s) 104, 109, 136, 291
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 2 cut(s) 199, 208
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 2 cut(s) 49, 154
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 98, 103, 202, 211, 278
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 3 cut(s) 158, 187, 202
HspAI GCGC 4 cut(s) 104, 109, 136, 291
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 193, 219
LpnPI CCDG 7 cut(s) 97, 113, 244, 257, 268, 271, 295
Lsp1109I GCAGC 4 cut(s) 93, 113, 120, 295
LweI GCATC 1 cut(s) 201
MaeI CTAG 3 cut(s) 54, 98, 335
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 198
MslI CAYNNNNRTG 2 cut(s) 188, 337
MspR9I CCNGG 2 cut(s) 259, 283
MteI GCGCNGCGC 1 cut(s) 107
MvaI CCWGG 2 cut(s) 259, 283
MvnI CGCG 4 cut(s) 149, 171, 219, 291
MwoI GCNNNNNNNGC 6 cut(s) 33, 98, 103, 202, 211, 278
NdeII GATC 1 cut(s) 76
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 3 cut(s) 158, 187, 202
NsbI TGCGCA 2 cut(s) 110, 137
NspI RCATGY 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 198
PkrI GCNGC 5 cut(s) 103, 108, 135, 280, 310
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 257, 281
PspGI CCWGG 2 cut(s) 257, 281
PspPI GGNCC 2 cut(s) 166, 229
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 2 cut(s) 188, 337
SacI GAGCTC 1 cut(s) 198
SalI GTCGAC 1 cut(s) 221
SatI GCNGC 5 cut(s) 102, 107, 134, 279, 309
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 2 cut(s) 166, 229
ScrFI CCNGG 2 cut(s) 259, 283
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 99, 116, 168, 192, 198
SfaNI GCATC 1 cut(s) 201
Sfr274I CTCGAG 1 cut(s) 206
SgrDI CGTCGACG 1 cut(s) 221
SinI GGWCC 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 188, 337
SmlI CTYRAG 2 cut(s) 206, 266
SmoI CTYRAG 2 cut(s) 206, 266
SpeI ACTAGT 1 cut(s) 334
SsiI CCGC 5 cut(s) 25, 34, 169, 217, 279
SspMI CTAG 3 cut(s) 54, 98, 335
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 257, 281
TaqI TCGA 2 cut(s) 207, 222
TauI GCSGC 1 cut(s) 281
TseI GCWGC 4 cut(s) 101, 106, 133, 308
TspGWI ACGGA 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 166
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 3 cut(s) 54, 98, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.