FvH4_4g13980

S-adenosylmethionine decarboxylase proenzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016174
Physical Location & Seq
Reverse (-)
17515604 .. 17516632
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13980.t1

Sequence Viewer

Length: 1029 bp
ATGGCTCTCTCTGGCTTTGAAGGTTTTGAAAAGCGTCTCGAGCTCCACTTCTTCGGGGACGACCCTAAAAATGTAGGGCTCGGCCTCAGACTCCTCGACTTCGAGATCCTAGACGAGCAAATCCTTCAGGCTGTGCAGTGCACCGTGGTCTCCGCCGTAGCCAATCACTACTTCGACGCCTACGTGTTGTCGGAGTCAAGTCTCTTTGTTTACCCTACCAAGATCATCATCAAGACATGTGGCACCACCGAGCTTCTCAAGTCAGTGGCGCCGCTGCTCCGCCACGCGTCGCTTCTCGGCCTCACTCTCACCTCCTGTCGCTACAGTCGCGGCAACTTTATCTTCCCCGCCGCACAGCCTTTTCCCCACGACACCTTCCAAAACGAGGTCGTTTACTTGGAAGACTCCCTCCCTGACTCCCTCTGCTACCGCAAGGCCTCCGTCATGCCCTCCAAGACACCTTCACACTCGTGGCATGTGTTTTCTGCTTCAACTGAAGCGACGTCGTATCCGCTCAACGGCGACGTAATGTACACCATTGAGATATGCATGACGGAGCTGGACCGTGACCTGGCGCGGAGCTTTTTTCAACATCCGGGGGATGGAAAGAACGGTGACACCGCCGGAAAAGAGATGACGGAGATAACCGGAATCGGAGAAATCATTCCCGGTGCTTTGATCTGCGACTTTGCTTTCGACCCTTGTGGATACTCCATGAATGGTATCGACGGCGATCGGCACTCCACCATTCATGTCACTCCTGAAGACGGGTTTAGTTACGCGAGCTATGAGTGCGTGGGGTCGGTGTACGACTGCGACGACGTCGTTCGGGTGCTGAAGAGGGTGTTCAAGGTGTTCCGGCCGGCGACCATGTCGGTATCCACCACGTGCGTGAGCCACGATGTGTGGACGCTGGTGGCCGGGGCTCTGGAGCCGCTAGGAATGAAGTGCCGGAGTTGCGCCGTGGATGAGTTCCCGGCTGCGGGGAATGTGGTGTACCAGACGTTCACGGCTCGCCGGAATAAGTAG

Protein Analysis

343

Amino Acids

37.75

Weight (kDa)

5.12

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 5 - 336 9.9e-104 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18930
fragaria_vesca FvH4_4g13980
malus_domestica MD13G1178800.v1.1 MD16G1180000.v1.1
prunus_persica Prupe.1G147600_v2.0.a1
pyrus_communis pycom16g15110
rosa_chinensis RchiOBHm_Chr4g0419671
rosa_laevigata RLG00000007803
rosa_multiflora Rmu_sc0003265.1_g000008
rosa_roxburghii Rroxscaffold_5G00362510
rosa_rugosa Rorug04G0159600
rosa_samantha Rh4BG223000 Rh4CG234300 Rh4DG220000
rosa_wichuraiana Rw4G019020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 506, 825
AccB1I GGYRCC 2 cut(s) 242, 268
AccBSI CCGCTC 1 cut(s) 514
AccII CGCG 4 cut(s) 287, 330, 577, 782
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 2 cut(s) 860, 918
AcuI CTGAAG 4 cut(s) 110, 516, 783, 857
AcvI CACGTG 1 cut(s) 888
AcyI GRCGYC 4 cut(s) 177, 269, 503, 822
AdeI CACNNNGTG 2 cut(s) 888, 904
AfaI GTAC 3 cut(s) 533, 809, 998
AfiI CCNNNNNNNGG 7 cut(s) 385, 518, 571, 602, 767, 982, 983
AflIII ACRYGT 3 cut(s) 183, 236, 285
AgsI TTSAA 5 cut(s) 20, 29, 492, 590, 850
AjnI CCWGG 1 cut(s) 570
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AleI CACNNNNGTG 2 cut(s) 469, 890
AluBI AGCT 5 cut(s) 43, 253, 559, 582, 786
AluI AGCT 5 cut(s) 43, 253, 559, 582, 786
Alw21I GWGCWC 2 cut(s) 45, 143
Alw26I GTCTC 3 cut(s) 41, 154, 206
Alw44I GTGCAC 1 cut(s) 139
AlwI GGATC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 5 cut(s) 82, 298, 435, 860, 918
ApaLI GTGCAC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 274, 980
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 3 cut(s) 271, 577, 962
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 4 cut(s) 597, 669, 922, 977
AsuHPI GGTGA 2 cut(s) 301, 626
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 562
BaeGI GKGCMC 1 cut(s) 143
BanI GGYRCC 2 cut(s) 242, 268
BanII GRGCYC 3 cut(s) 45, 81, 928
BauI CACGAG 1 cut(s) 469
BbrPI CACGTG 1 cut(s) 888
BbsI GAAGAC 2 cut(s) 408, 771
Bbv12I GWGCWC 2 cut(s) 45, 143
BbvI GCAGC 2 cut(s) 261, 967
BccI CCATC 1 cut(s) 596
BceAI ACGGC 5 cut(s) 140, 535, 745, 947, 1026
BciT130I CCWGG 1 cut(s) 572
BciVI GTATCC 3 cut(s) 519, 701, 889
BcnI CCSGG 4 cut(s) 597, 669, 922, 977
BcoDI GTCTC 3 cut(s) 41, 154, 206
BfaI CTAG 2 cut(s) 110, 938
BfmI CTRYAG 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 272
BfuI GTATCC 3 cut(s) 519, 701, 889
BisI GCNGC 6 cut(s) 272, 275, 331, 351, 935, 981
BlsI GCNGC 6 cut(s) 273, 276, 332, 352, 936, 982
Bme1390I CCNGG 5 cut(s) 572, 597, 669, 922, 977
Bme18I GGWCC 1 cut(s) 562
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 3 cut(s) 244, 270, 933
BmrFI CCNGG 5 cut(s) 572, 597, 669, 922, 977
BpiI GAAGAC 2 cut(s) 408, 771
BpmI CTGGAG 1 cut(s) 950
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 4 cut(s) 597, 669, 922, 977
BsaAI YACGTR 2 cut(s) 184, 888
BsaBI GATNNNNATC 1 cut(s) 227
BsaHI GRCGYC 4 cut(s) 177, 269, 503, 822
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 4 cut(s) 144, 596, 921, 963
BsaWI WCCGGW 1 cut(s) 647
Bsc4I CCNNNNNNNGG 7 cut(s) 385, 518, 571, 602, 767, 982, 983
Bse118I RCCGGY 1 cut(s) 862
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 4 cut(s) 144, 596, 921, 963
BseGI GGATG 3 cut(s) 592, 607, 973
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 7 cut(s) 385, 518, 571, 602, 767, 982, 983
BseMII CTCAG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 143
BseX3I CGGCCG 1 cut(s) 860
BseXI GCAGC 2 cut(s) 261, 967
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 4 cut(s) 287, 330, 577, 782
Bsh1285I CGRYCG 2 cut(s) 736, 863
BshFI GGCC 5 cut(s) 84, 300, 437, 862, 920
BshNI GGYRCC 2 cut(s) 242, 268
BsiEI CGRYCG 2 cut(s) 736, 863
BsiHKAI GWGCWC 2 cut(s) 45, 143
BsiHKCI CYCGRG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 7 cut(s) 385, 518, 571, 602, 767, 982, 983
BsmAI GTCTC 3 cut(s) 41, 154, 206
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 5 cut(s) 84, 300, 437, 862, 920
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 4 cut(s) 45, 81, 143, 928
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 4 cut(s) 105, 222, 678, 733
BspANI GGCC 5 cut(s) 84, 300, 437, 862, 920
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 4 cut(s) 287, 330, 577, 782
BspLI GGNNCC 3 cut(s) 244, 270, 933
BspPI GGATC 1 cut(s) 100
BspT107I GGYRCC 2 cut(s) 242, 268
BspTNI GGTCTC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 862
BsrGI TGTACA 1 cut(s) 531
BssAI RCCGGY 1 cut(s) 862
BssECI CCNNGG 4 cut(s) 144, 596, 921, 963
BssMI GATC 4 cut(s) 105, 222, 678, 733
BssNI GRCGYC 4 cut(s) 177, 269, 503, 822
BssSI CACGAG 1 cut(s) 469
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 4 cut(s) 145, 326, 566, 614
Bst6I CTCTTC 1 cut(s) 833
BstACI GRCGYC 4 cut(s) 177, 269, 503, 822
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 2 cut(s) 184, 888
BstC8I GCNNGC 3 cut(s) 784, 864, 1015
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 2 cut(s) 144, 963
BstF5I GGATG 3 cut(s) 592, 607, 973
BstFNI CGCG 4 cut(s) 287, 330, 577, 782
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 3 cut(s) 271, 577, 962
BstKTI GATC 4 cut(s) 108, 225, 681, 736
BstMAI GTCTC 3 cut(s) 41, 154, 206
BstMBI GATC 4 cut(s) 105, 222, 678, 733
BstMCI CGRYCG 2 cut(s) 736, 863
BstMWI GCNNNNNNNGC 4 cut(s) 40, 327, 792, 957
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 240, 479
BstSCI CCNGG 5 cut(s) 570, 595, 667, 920, 975
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 4 cut(s) 287, 330, 577, 782
BstV1I GCAGC 2 cut(s) 261, 967
BstV2I GAAGAC 2 cut(s) 408, 771
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BstZI CGGCCG 1 cut(s) 860
BsuI GTATCC 3 cut(s) 519, 701, 889
BsuRI GGCC 5 cut(s) 84, 300, 437, 862, 920
BtgI CCRYGG 2 cut(s) 144, 963
BtsCI GGATG 3 cut(s) 592, 607, 973
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 2 cut(s) 143, 270
Cac8I GCNNGC 3 cut(s) 784, 864, 1015
CfoI GCGC 3 cut(s) 271, 577, 962
Cfr10I RCCGGY 1 cut(s) 862
Cfr13I GGNCC 1 cut(s) 562
CseI GACGC 4 cut(s) 23, 185, 276, 919
Csp6I GTAC 3 cut(s) 532, 808, 997
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 7 cut(s) 237, 445, 476, 550, 715, 752, 871
CviQI GTAC 3 cut(s) 532, 808, 997
DdeI CTNAG 1 cut(s) 86
DinI GGCGCC 1 cut(s) 270
DpnI GATC 4 cut(s) 107, 224, 680, 735
DpnII GATC 4 cut(s) 105, 222, 678, 733
DraIII CACNNNGTG 2 cut(s) 888, 904
EaeI YGGCCR 2 cut(s) 860, 918
EagI CGGCCG 1 cut(s) 860
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
EciI GGCGGA 2 cut(s) 142, 269
Ecl136II GAGCTC 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 860
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 3 cut(s) 45, 81, 928
Eco31I GGTCTC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 562
Eco52I CGGCCG 1 cut(s) 860
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 4 cut(s) 110, 516, 783, 857
Eco72I CACGTG 1 cut(s) 888
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 3 cut(s) 45, 81, 928
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 7 cut(s) 240, 448, 479, 553, 718, 755, 874
FaiI YATR 9 cut(s) 238, 446, 477, 547, 551, 716, 753, 789, 872
FaqI GGGAC 1 cut(s) 71
FatI CATG 7 cut(s) 236, 444, 475, 549, 714, 751, 870
FauI CCCGC 2 cut(s) 355, 976
Fnu4HI GCNGC 6 cut(s) 272, 275, 331, 351, 935, 981
FokI GGATG 3 cut(s) 579, 614, 980
FriOI GRGCYC 3 cut(s) 45, 81, 928
Fsp4HI GCNGC 6 cut(s) 272, 275, 331, 351, 935, 981
FspBI CTAG 2 cut(s) 110, 938
GlaI GCGC 3 cut(s) 270, 576, 961
GluI GCNGC 6 cut(s) 272, 275, 331, 351, 935, 981
GsuI CTGGAG 1 cut(s) 950
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 5 cut(s) 84, 300, 437, 862, 920
HgaI GACGC 4 cut(s) 23, 185, 276, 919
HhaI GCGC 3 cut(s) 271, 577, 962
Hin1I GRCGYC 4 cut(s) 177, 269, 503, 822
Hin1II CATG 7 cut(s) 240, 448, 479, 553, 718, 755, 874
Hin6I GCGC 3 cut(s) 269, 575, 960
HinP1I GCGC 3 cut(s) 269, 575, 960
HinfI GANTC 5 cut(s) 90, 194, 404, 416, 651
HphI GGTGA 2 cut(s) 301, 626
Hpy166II GTNNAC 8 cut(s) 141, 211, 394, 534, 808, 909, 997, 1008
Hpy188I TCNGA 3 cut(s) 89, 193, 656
Hpy188III TCNNGA 5 cut(s) 38, 103, 232, 761, 929
Hpy8I GTNNAC 8 cut(s) 141, 211, 394, 534, 808, 909, 997, 1008
Hpy99I CGWCG 9 cut(s) 179, 292, 505, 508, 527, 731, 821, 824, 827
HpyAV CCTTC 4 cut(s) 14, 134, 385, 471
HpyCH4III ACNGT 4 cut(s) 145, 326, 566, 614
HpyCH4IV ACGT 6 cut(s) 183, 503, 525, 822, 887, 1004
HpyCH4V TGCA 3 cut(s) 136, 141, 549
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 327, 792, 957
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 6 cut(s) 183, 503, 525, 822, 887, 1004
Hsp92I GRCGYC 4 cut(s) 177, 269, 503, 822
Hsp92II CATG 7 cut(s) 240, 448, 479, 553, 718, 755, 874
HspAI GCGC 3 cut(s) 269, 575, 960
KasI GGCGCC 1 cut(s) 268
KroI GCCGGC 1 cut(s) 862
KroNI GCCGGC 1 cut(s) 864
Kzo9I GATC 4 cut(s) 105, 222, 678, 733
LmnI GCTCC 5 cut(s) 48, 282, 556, 579, 931
Lsp1109I GCAGC 2 cut(s) 261, 967
MaeI CTAG 2 cut(s) 110, 938
MaeII ACGT 6 cut(s) 183, 503, 525, 822, 887, 1004
MaeIII GTNAC 4 cut(s) 566, 614, 754, 776
MalI GATC 4 cut(s) 107, 224, 680, 735
MbiI CCGCTC 1 cut(s) 514
MboI GATC 4 cut(s) 105, 222, 678, 733
MboII GAAGA 5 cut(s) 43, 334, 413, 776, 850
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 4 cut(s) 45, 81, 143, 928
MluI ACGCGT 1 cut(s) 285
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 4 cut(s) 84, 203, 398, 410
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 551
MroNI GCCGGC 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 663
MslI CAYNNNNRTG 2 cut(s) 469, 890
MspA1I CMGCKG 1 cut(s) 274
MspR9I CCNGG 5 cut(s) 572, 597, 669, 922, 977
MvaI CCWGG 1 cut(s) 572
MvnI CGCG 4 cut(s) 287, 330, 577, 782
MwoI GCNNNNNNNGC 4 cut(s) 40, 327, 792, 957
NaeI GCCGGC 1 cut(s) 864
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 4 cut(s) 597, 669, 922, 977
NdeII GATC 4 cut(s) 105, 222, 678, 733
NgoMIV GCCGGC 1 cut(s) 862
NlaIII CATG 7 cut(s) 240, 448, 479, 553, 718, 755, 874
NlaIV GGNNCC 3 cut(s) 244, 270, 933
NmeAIII GCCGAG 2 cut(s) 60, 276
NmuCI GTSAC 3 cut(s) 566, 614, 754
NsiI ATGCAT 1 cut(s) 551
NspI RCATGY 2 cut(s) 240, 479
OliI CACNNNNGTG 2 cut(s) 469, 890
PaeR7I CTCGAG 1 cut(s) 38
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 816
PdiI GCCGGC 1 cut(s) 864
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 651
PflFI GACNNNGTC 2 cut(s) 821, 871
PkrI GCNGC 6 cut(s) 273, 276, 332, 352, 936, 982
Ple19I CGATCG 1 cut(s) 736
PleI GAGTC 4 cut(s) 84, 202, 398, 410
PluTI GGCGCC 1 cut(s) 272
PmaCI CACGTG 1 cut(s) 888
PmlI CACGTG 1 cut(s) 888
PpsI GAGTC 4 cut(s) 84, 202, 398, 410
Ppu21I YACGTR 2 cut(s) 184, 888
PscI ACATGT 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 570
PspCI CACGTG 1 cut(s) 888
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 3 cut(s) 244, 270, 933
PspPI GGNCC 1 cut(s) 562
PsrI GAACNNNNNNTAC 2 cut(s) 989, 1021
PsuI RGATCY 1 cut(s) 105
PsyI GACNNNGTC 2 cut(s) 821, 871
PvuI CGATCG 1 cut(s) 736
RsaI GTAC 3 cut(s) 533, 809, 998
RsaNI GTAC 3 cut(s) 532, 808, 997
RseI CAYNNNNRTG 2 cut(s) 469, 890
SacI GAGCTC 1 cut(s) 45
SatI GCNGC 6 cut(s) 272, 275, 331, 351, 935, 981
Sau3AI GATC 4 cut(s) 105, 222, 678, 733
Sau96I GGNCC 1 cut(s) 562
SchI GAGTC 4 cut(s) 84, 203, 398, 410
ScrFI CCNGG 5 cut(s) 572, 597, 669, 922, 977
SduI GDGCHC 4 cut(s) 45, 81, 143, 928
SfcI CTRYAG 1 cut(s) 322
SfoI GGCGCC 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 1 cut(s) 562
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 2 cut(s) 469, 890
SmlI CTYRAG 2 cut(s) 38, 257
SmoI CTYRAG 2 cut(s) 38, 257
SseBI AGGCCT 1 cut(s) 437
SspDI GGCGCC 1 cut(s) 268
SspMI CTAG 2 cut(s) 110, 938
SstI GAGCTC 1 cut(s) 45
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 5 cut(s) 570, 595, 667, 920, 975
TaaI ACNGT 4 cut(s) 145, 326, 566, 614
TaiI ACGT 6 cut(s) 186, 506, 528, 825, 890, 1007
TaqI TCGA 6 cut(s) 39, 96, 102, 174, 696, 726
TatI WGTACW 1 cut(s) 531
TauI GCSGC 4 cut(s) 274, 333, 353, 937
TfiI GAWTC 1 cut(s) 651
TscAI CASTG 2 cut(s) 143, 270
TseFI GTSAC 3 cut(s) 566, 614, 754
TseI GCWGC 2 cut(s) 274, 980
Tsp45I GTSAC 3 cut(s) 566, 614, 754
TspDTI ATGAA 3 cut(s) 731, 740, 959
TspGWI ACGGA 3 cut(s) 430, 569, 653
TspRI CASTG 2 cut(s) 143, 270
Tth111I GACNNNGTC 2 cut(s) 821, 871
VneI GTGCAC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 562
XceI RCATGY 2 cut(s) 240, 479
XhoI CTCGAG 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 2 cut(s) 110, 938
ZraI GACGTC 2 cut(s) 504, 823
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.