FvH4_4g15932

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019698
Physical Location & Seq
Reverse (-)
19891556 .. 19893887
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15932.t1

Sequence Viewer

Length: 1008 bp
ATGGCGAGGTTGAGGATGCGATACATGTCGATGAGGTGCCCAAAAATGTGTCGTGCTTGTGCCAGCACTCACCCGCTCCAACTGACTCAAGGACTCACTCAAGGACTCGTACTAGCTTTGGCAGATTCTCGTCGATTGACTCGTACTAGATCGACTTGTGCCAGCACTGACCCGCTCCAGCTCACCGCGCAAGAATGGACGGCCAATTCTAGATCGACTCGTACTAGCTGTGTCACATCTGGAACACCCTCCCCTCGGCTTCCTTTTTCAGTTTTTTGTTGCTGTCTAAACGATGGCTATGTACTGCGGGGATGGTGGCTTCAACAAGGGCGCGCAACTAGTACCGGCGGTGGATTGAGGCTATTTCAGGTGTCATGTTGCTTGGGTTCAATGGCTACGGGTTGTTTGGATCGTGGCGAATTCGAGGTGCATGGTCATGGCGCTAATATCTGCTATGGGGTGTTCGATGTCGGATGTGTGGCTATTGTTTGCCCTGGCATGGCCGCATGGGATGTCGTAATGGTGGGATGTGGGGAGGTGGTCAAATCGACCGGACTCACCCCACATGCGGTGTCAACCGTCCTCCTTCTCACCGGCCTAGTCGGCGGTGGCGGGGAAGAAGCTTTCATAGCCCAGCGACGGGTTGCGGAAGCCAGTCTGGTTTTCCATCATCGATCGCTGATGGTGTTGCATAGAAACACAGAGAAGATGGTGGCCATTGCGATCGGTGCTGCTTCGGAATGGGAGGAACTTACAGTTTTAGATGAAGAGCTTCCATTCAAATCGCTCAAGAGTCTGGAAGAGTTGAAGGCTTTCGTCGATTCGACTGACAAAGCTTTGGTTCTATTTGAGTCTTGTGGCTGGACTCCGAAATTGATGGCCAGGAGGAAGATGAATGGGACTGATCACAGTGGTTTTGGTACACAATGTTCATTCTTCATTCTTTTGATGCAGTTAATTTGGAGAGTTTTAGCATTGTGGTATTGGATGGCGCATTATCTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

336

Amino Acids

36.83

Weight (kDa)

8.67

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g15891 FvH4_4g15932 FvH4_5g30901 FvH4_6g37553
rosa_chinensis RchiOBHm_MTg0498701
rosa_samantha Rh1CG096700 Rh6AG380000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 36
AccBSI CCGCTC 2 cut(s) 76, 175
AccII CGCG 2 cut(s) 188, 333
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 4 cut(s) 201, 501, 714, 879
AcsI RAATTY 1 cut(s) 419
AfaI GTAC 6 cut(s) 111, 145, 223, 303, 343, 922
AfiI CCNNNNNNNGG 5 cut(s) 255, 499, 568, 639, 640
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 4 cut(s) 323, 390, 781, 808
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 2 cut(s) 493, 881
AluBI AGCT 6 cut(s) 116, 181, 228, 623, 772, 836
AluI AGCT 6 cut(s) 116, 181, 228, 623, 772, 836
AlwI GGATC 1 cut(s) 417
AoxI GGCC 5 cut(s) 201, 501, 595, 714, 879
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 1 cut(s) 419
Asp700I GAANNNNTTC 2 cut(s) 771, 812
AspLEI GCGC 5 cut(s) 190, 333, 335, 443, 994
AsuHPI GGTGA 4 cut(s) 62, 175, 550, 583
BaeGI GKGCMC 1 cut(s) 41
BalI TGGCCA 2 cut(s) 716, 881
BanI GGYRCC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 718
BccI CCATC 7 cut(s) 287, 306, 675, 676, 703, 871, 982
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 1 cut(s) 983
BciT130I CCWGG 2 cut(s) 495, 883
BclI TGATCA 1 cut(s) 904
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 6 cut(s) 113, 147, 210, 225, 339, 599
BfoI RGCGCY 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 603
BisI GCNGC 2 cut(s) 504, 732
BlsI GCNGC 2 cut(s) 505, 733
Bme1390I CCNGG 2 cut(s) 495, 883
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 2 cut(s) 495, 883
BmsI GCATC 2 cut(s) 6, 939
BpmI CTGGAG 1 cut(s) 161
BpuEI CTTGAG 3 cut(s) 72, 84, 773
Bsa29I ATCGAT 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 254, 493
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 499, 568, 639, 640
Bse118I RCCGGY 2 cut(s) 344, 593
Bse1I ACTGG 1 cut(s) 654
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 2 cut(s) 495, 883
BseCI ATCGAT 1 cut(s) 673
BseDI CCNNGG 2 cut(s) 254, 493
BseGI GGATG 6 cut(s) 21, 317, 479, 517, 533, 993
BseLI CCNNNNNNNGG 5 cut(s) 255, 499, 568, 639, 640
BseMI GCAATG 1 cut(s) 717
BseNI ACTGG 1 cut(s) 654
BsePI GCGCGC 1 cut(s) 331
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 1 cut(s) 718
BseYI CCCAGC 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 188, 333
Bsh1285I CGRYCG 3 cut(s) 552, 677, 726
BshFI GGCC 5 cut(s) 203, 503, 597, 716, 881
BshNI GGYRCC 1 cut(s) 36
BshVI ATCGAT 1 cut(s) 673
BsiEI CGRYCG 3 cut(s) 552, 677, 726
BsiSI CCGG 3 cut(s) 345, 552, 594
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 5 cut(s) 255, 499, 568, 639, 640
BsmFI GGGAC 1 cut(s) 913
BsnI GGCC 5 cut(s) 203, 503, 597, 716, 881
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 6 cut(s) 149, 212, 409, 674, 723, 904
BspANI GGCC 5 cut(s) 203, 503, 597, 716, 881
BspDI ATCGAT 1 cut(s) 673
BspFNI CGCG 2 cut(s) 188, 333
BspLI GGNNCC 1 cut(s) 38
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 762
BspT107I GGYRCC 1 cut(s) 36
BsrBI CCGCTC 2 cut(s) 76, 175
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 2 cut(s) 344, 593
BsrI ACTGG 1 cut(s) 654
BssAI RCCGGY 2 cut(s) 344, 593
BssECI CCNNGG 2 cut(s) 254, 493
BssHII GCGCGC 1 cut(s) 331
BssMI GATC 6 cut(s) 149, 212, 409, 674, 723, 904
Bst2UI CCWGG 2 cut(s) 495, 883
Bst4CI ACNGT 3 cut(s) 580, 757, 911
Bst6I CTCTTC 2 cut(s) 762, 795
BstC8I GCNNGC 3 cut(s) 64, 163, 333
BstF5I GGATG 6 cut(s) 21, 317, 479, 517, 533, 993
BstFNI CGCG 2 cut(s) 188, 333
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 5 cut(s) 190, 333, 335, 443, 994
BstKTI GATC 6 cut(s) 152, 215, 412, 677, 726, 907
BstMBI GATC 6 cut(s) 149, 212, 409, 674, 723, 904
BstMCI CGRYCG 3 cut(s) 552, 677, 726
BstMWI GCNNNNNNNGC 4 cut(s) 187, 603, 629, 728
BstNI CCWGG 2 cut(s) 495, 883
BstNSI RCATGY 2 cut(s) 28, 569
BstSCI CCNGG 2 cut(s) 493, 881
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 2 cut(s) 188, 333
BstV1I GCAGC 1 cut(s) 718
Bsu15I ATCGAT 1 cut(s) 673
BsuRI GGCC 5 cut(s) 203, 503, 597, 716, 881
BsuTUI ATCGAT 1 cut(s) 673
BtsCI GGATG 6 cut(s) 21, 317, 479, 517, 533, 993
BtsIMutI CAGTG 2 cut(s) 165, 916
Cac8I GCNNGC 3 cut(s) 64, 163, 333
CfoI GCGC 5 cut(s) 190, 333, 335, 443, 994
Cfr10I RCCGGY 2 cut(s) 344, 593
ClaI ATCGAT 1 cut(s) 673
Csp6I GTAC 6 cut(s) 110, 144, 222, 302, 342, 921
CviAII CATG 7 cut(s) 25, 375, 431, 437, 499, 507, 566
CviQI GTAC 6 cut(s) 110, 144, 222, 302, 342, 921
DpnI GATC 6 cut(s) 151, 214, 411, 676, 725, 906
DpnII GATC 6 cut(s) 149, 212, 409, 674, 723, 904
EaeI YGGCCR 4 cut(s) 201, 501, 714, 879
Eam1104I CTCTTC 2 cut(s) 762, 795
EarI CTCTTC 2 cut(s) 762, 795
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 493, 881
FaeI CATG 7 cut(s) 28, 378, 434, 440, 502, 510, 569
FaqI GGGAC 1 cut(s) 913
FatI CATG 7 cut(s) 24, 374, 430, 436, 498, 506, 565
FauI CCCGC 4 cut(s) 81, 180, 300, 605
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 2 cut(s) 504, 732
FokI GGATG 6 cut(s) 28, 324, 486, 524, 540, 1000
Fsp4HI GCNGC 2 cut(s) 504, 732
FspBI CTAG 6 cut(s) 113, 147, 210, 225, 339, 599
GlaI GCGC 5 cut(s) 189, 332, 334, 442, 993
GluI GCNGC 2 cut(s) 504, 732
GsaI CCCAGC 1 cut(s) 637
GsuI CTGGAG 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 5 cut(s) 203, 503, 597, 716, 881
HapII CCGG 3 cut(s) 345, 552, 594
HhaI GCGC 5 cut(s) 190, 333, 335, 443, 994
Hin1II CATG 7 cut(s) 28, 378, 434, 440, 502, 510, 569
Hin6I GCGC 5 cut(s) 188, 331, 333, 441, 992
HinP1I GCGC 5 cut(s) 188, 331, 333, 441, 992
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HindIII AAGCTT 2 cut(s) 621, 834
HpaII CCGG 3 cut(s) 345, 552, 594
HphI GGTGA 4 cut(s) 62, 175, 550, 583
Hpy166II GTNNAC 2 cut(s) 576, 923
Hpy188I TCNGA 3 cut(s) 473, 739, 870
Hpy188III TCNNGA 4 cut(s) 210, 240, 790, 797
Hpy8I GTNNAC 2 cut(s) 576, 923
Hpy99I CGWCG 3 cut(s) 135, 642, 821
HpyAV CCTTC 2 cut(s) 596, 802
HpyCH4III ACNGT 3 cut(s) 580, 757, 911
HpyCH4V TGCA 3 cut(s) 430, 691, 952
HpyF10VI GCNNNNNNNGC 4 cut(s) 187, 603, 629, 728
Hsp92II CATG 7 cut(s) 28, 378, 434, 440, 502, 510, 569
HspAI GCGC 5 cut(s) 188, 331, 333, 441, 992
Ksp22I TGATCA 1 cut(s) 904
Kzo9I GATC 6 cut(s) 149, 212, 409, 674, 723, 904
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 2 cut(s) 81, 180
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 2 cut(s) 6, 939
MaeI CTAG 6 cut(s) 113, 147, 210, 225, 339, 599
MaeIII GTNAC 1 cut(s) 232
MalI GATC 6 cut(s) 151, 214, 411, 676, 725, 906
MbiI CCGCTC 2 cut(s) 76, 175
MboI GATC 6 cut(s) 149, 212, 409, 674, 723, 904
MboII GAAGA 7 cut(s) 629, 718, 779, 812, 901, 928, 992
MhlI GDGCHC 1 cut(s) 41
MlsI TGGCCA 2 cut(s) 716, 881
MluCI AATT 4 cut(s) 205, 419, 872, 957
MluNI TGGCCA 2 cut(s) 716, 881
MlyI GAGTC 9 cut(s) 79, 87, 99, 133, 211, 549, 802, 859, 860
MmeI TCCRAC 2 cut(s) 103, 451
Mox20I TGGCCA 2 cut(s) 716, 881
MroXI GAANNNNTTC 2 cut(s) 771, 812
MscI TGGCCA 2 cut(s) 716, 881
MseI TTAA 1 cut(s) 956
MslI CAYNNNNRTG 2 cut(s) 29, 435
Msp20I TGGCCA 2 cut(s) 716, 881
MspI CCGG 3 cut(s) 345, 552, 594
MspR9I CCNGG 2 cut(s) 495, 883
MvaI CCWGG 2 cut(s) 495, 883
MvnI CGCG 2 cut(s) 188, 333
MwoI GCNNNNNNNGC 4 cut(s) 187, 603, 629, 728
NdeII GATC 6 cut(s) 149, 212, 409, 674, 723, 904
NlaIII CATG 7 cut(s) 28, 378, 434, 440, 502, 510, 569
NlaIV GGNNCC 1 cut(s) 38
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 2 cut(s) 28, 569
PauI GCGCGC 1 cut(s) 331
PciI ACATGT 1 cut(s) 24
PciSI GCTCTTC 1 cut(s) 762
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PdmI GAANNNNTTC 2 cut(s) 771, 812
PfeI GAWTC 2 cut(s) 125, 821
PkrI GCNGC 2 cut(s) 505, 733
Ple19I CGATCG 2 cut(s) 677, 726
PleI GAGTC 9 cut(s) 79, 87, 99, 133, 211, 549, 801, 859, 859
PpsI GAGTC 9 cut(s) 79, 87, 99, 133, 211, 549, 801, 859, 859
PscI ACATGT 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 493, 881
PspFI CCCAGC 1 cut(s) 633
PspGI CCWGG 2 cut(s) 493, 881
PspN4I GGNNCC 1 cut(s) 38
PsrI GAACNNNNNNTAC 2 cut(s) 913, 945
PteI GCGCGC 1 cut(s) 331
PvuI CGATCG 2 cut(s) 677, 726
RsaI GTAC 6 cut(s) 111, 145, 223, 303, 343, 922
RsaNI GTAC 6 cut(s) 110, 144, 222, 302, 342, 921
RseI CAYNNNNRTG 2 cut(s) 29, 435
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 1 cut(s) 956
SatI GCNGC 2 cut(s) 504, 732
Sau3AI GATC 6 cut(s) 149, 212, 409, 674, 723, 904
SchI GAGTC 9 cut(s) 79, 87, 99, 133, 211, 549, 802, 859, 860
ScrFI CCNGG 2 cut(s) 495, 883
SduI GDGCHC 1 cut(s) 41
SfaNI GCATC 2 cut(s) 6, 939
SmiMI CAYNNNNRTG 2 cut(s) 29, 435
SmlI CTYRAG 3 cut(s) 87, 99, 788
SmoI CTYRAG 3 cut(s) 87, 99, 788
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 4 cut(s) 205, 419, 872, 957
SspMI CTAG 6 cut(s) 113, 147, 210, 225, 339, 599
StyD4I CCNGG 2 cut(s) 493, 881
TaaI ACNGT 3 cut(s) 580, 757, 911
TasI AATT 4 cut(s) 205, 419, 872, 957
TatI WGTACW 1 cut(s) 301
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 2 cut(s) 125, 821
Tru1I TTAA 1 cut(s) 956
Tru9I TTAA 1 cut(s) 956
TscAI CASTG 2 cut(s) 172, 916
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 731
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 5 cut(s) 616, 780, 908, 921, 928
TspRI CASTG 2 cut(s) 172, 916
XapI RAATTY 1 cut(s) 419
XbaI TCTAGA 1 cut(s) 209
XceI RCATGY 2 cut(s) 28, 569
XmnI GAANNNNTTC 2 cut(s) 771, 812
XspI CTAG 6 cut(s) 113, 147, 210, 225, 339, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.