FvH4_4g19590

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016309
Physical Location & Seq
Reverse (-)
23198091 .. 23200562
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19590.t1

Sequence Viewer

Length: 1332 bp
ATGGATCAAATTGAGGATTTTAAAAAGATGGAAGACATTAGCAGATCAGAGGGACAGTCACAAACCAGAGACCACATCTCTCTTGAAATCACTTCTGAAAGTGGTGGACTTGTATCTCAGATCAAAGAAAAAATGGAAAACATTGCTGTATCAGTATCTATCTTCAGAGTCCCTAACGAAAAAAAATGTGCCCCGGATTATGTCTCCATTGGCCCTTTGCACTATAAACAGTTACGTGATAGCAAAGTCTCGGAGGATGATAAGTGGCGCTACTGCTATGCACTCCTCAATCGAAAGCCAAATCTAGAAGCAAGCCTCGACACCTGCGTGAAAGCCCTGAAACAAATGGAGAACAAGGCACGAAGATGTTACAATGAAGACATCAGTCTCACTAGTGATGAATTTGTGCAGTTGATGCTAATTGACACCTGCTTCATCATTGAGCTATTTCTCAAGTATTCATACAAGAGCCTTAGGTCTCGCCGTGATCCCATCTTCAATAGCCCTGGTATGCTCATAGAATTGAGATGTAACATGGTGGTACTCGAAAACCAAATCCCCTTCTTTGTTGTTCAAAGGTTGTTCCAATTAGTACCACTTCCAACACAATGTACCGAGTCCCTCAGCGAACTTGCTACACGTTTCTTCAAGTACCTTCTACCAGGAGAGTATCGTCGTGAGCAAGAAGGGCATCATTTACTCGATCTAATTCGACACTGCATCCTCCCAACACATCATAAGCTACAATCGACAGGCAAGAAAACTCCGGACTACTTGGATTGCGTAAAGAAGCTAAAAAGAGCAGGGGTTAAATTCCAATGTGCTACCGTGGTACACAGTTTCTTGGATGTCAAGTTTACTAATGGTGTGTTCAAAATGCCACCTCTTTTAGTCCATCACTGCACTGAAACACTCCTCAAGAACCTCATTGCACTCGAGCAGCGACACATTGGGGATGATCCGGTGCAGCATATCACATCTTATGCATACTTAATGGGGTGCCTGATCCAGTCCGAGAAAGATGTGAAATTGCTGCGGAAAAAACAGATTCTTGTGCATGAGGAGAAGAATGACAAGGAGGTTTTTGAAGTGTTGAAGAAGTTGTGCGAGCAGATCGATTTGAAGGATTTTTACTATGTGAGGCTTTTTGATGAGGTGGGTGAATTTATGAAGAGAAAGAGCTGGCACACGAAGAAGCAAAAGTTGAAGAGTACATACCATCCCAAGACTCCTTCGGCCGTTGCGGTGCTTGTTGTTGCCGTTTTGGCTCTTCTTCTCACATTTGTTGGAGCTTTCTTTTCTATACTCACATTCGCTCGCCACCATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

444

Amino Acids

51.51

Weight (kDa)

8.84

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 54 - 426 1.4e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19590
malus_domestica MD16G1154900.v1.1
prunus_persica Prupe.1G118600_v2.0.a1
pyrus_communis pycom16g13130
rosa_chinensis RchiOBHm_Chr4g0424851
rosa_laevigata RLG00000007415
rosa_multiflora Rmu_sc0006754.1_g000008
rosa_roxburghii Rroxscaffold_5G00367070
rosa_rugosa Rorug04G0199600 Rorug04G0199700
rosa_samantha Rh4AG257400 Rh4BG262800 Rh4CG273700 Rh4DG258500
rosa_wichuraiana Rw4G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 332, 437
Acc36I ACCTGC 2 cut(s) 332, 437
AccB1I GGYRCC 1 cut(s) 999
AccIII TCCGGA 1 cut(s) 766
AciI CCGC 2 cut(s) 1036, 1244
AclWI GGATC 4 cut(s) 12, 482, 953, 1000
AcoI YGGCCR 1 cut(s) 1236
AcsI RAATTY 3 cut(s) 401, 812, 1163
AcuI CTGAAG 1 cut(s) 148
AfaI GTAC 6 cut(s) 543, 594, 613, 653, 834, 1213
AflIII ACRYGT 1 cut(s) 638
AgsI TTSAA 9 cut(s) 86, 499, 575, 649, 874, 1088, 1096, 1123, 1207
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 2 cut(s) 505, 661
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 5 cut(s) 445, 742, 793, 1182, 1292
AluI AGCT 5 cut(s) 445, 742, 793, 1182, 1292
Alw26I GTCTC 5 cut(s) 63, 208, 253, 392, 483
AlwI GGATC 4 cut(s) 12, 482, 953, 1000
Ama87I CYCGRG 1 cut(s) 935
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 2 cut(s) 211, 1236
ApeKI GCWGC 3 cut(s) 940, 967, 1033
ApoI RAATTY 3 cut(s) 401, 812, 1163
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 1172
AvaI CYCGRG 1 cut(s) 935
AxyI CCTNAGG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 999
BarI GAAGNNNNNNTAC 4 cut(s) 1115, 1147, 1199, 1231
BbsI GAAGAC 2 cut(s) 39, 384
BbvCI CCTCAGC 1 cut(s) 623
BbvI GCAGC 3 cut(s) 952, 979, 1020
BccI CCATC 4 cut(s) 22, 500, 903, 1227
BceAI ACGGC 3 cut(s) 468, 1223, 1244
BciT130I CCWGG 2 cut(s) 507, 663
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 5 cut(s) 63, 208, 253, 392, 483
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 305, 393
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 2 cut(s) 332, 437
BglI GCCNNNNNGGC 1 cut(s) 1265
BisI GCNGC 3 cut(s) 941, 968, 1034
BlsI GCNGC 3 cut(s) 942, 969, 1035
Bme1390I CCNGG 3 cut(s) 194, 507, 663
BmeT110I CYCGRG 1 cut(s) 935
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 1001
BmrFI CCNGG 3 cut(s) 194, 507, 663
BmsI GCATC 3 cut(s) 405, 700, 729
BoxI GACNNNNGTC 1 cut(s) 384
BpiI GAAGAC 2 cut(s) 39, 384
Bpu10I CCTNAGC 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 437, 902
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 1116
BsaAI YACGTR 1 cut(s) 236
BsaI GGTCTC 2 cut(s) 63, 483
BsaJI CCNNGG 3 cut(s) 192, 505, 828
BsaWI WCCGGW 2 cut(s) 766, 961
Bse1I ACTGG 1 cut(s) 1009
Bse21I CCTNAGG 1 cut(s) 473
Bse3DI GCAATG 2 cut(s) 141, 927
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 2 cut(s) 507, 663
BseCI ATCGAT 1 cut(s) 1116
BseDI CCNNGG 3 cut(s) 192, 505, 828
BseGI GGATG 5 cut(s) 262, 720, 853, 961, 1219
BseMI GCAATG 2 cut(s) 141, 927
BseMII CTCAG 2 cut(s) 131, 637
BseNI ACTGG 1 cut(s) 1009
BseRI GAGGAG 3 cut(s) 275, 905, 1076
BseSI GKGCMC 1 cut(s) 193
BseX3I CGGCCG 1 cut(s) 1236
BseXI GCAGC 3 cut(s) 952, 979, 1020
BsgI GTGCAG 3 cut(s) 428, 886, 986
Bsh1285I CGRYCG 1 cut(s) 1239
BshFI GGCC 2 cut(s) 213, 1238
BshNI GGYRCC 1 cut(s) 999
BshVI ATCGAT 1 cut(s) 1116
BsiEI CGRYCG 1 cut(s) 1239
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 3 cut(s) 194, 767, 962
BslFI GGGAC 3 cut(s) 66, 155, 604
BsmAI GTCTC 5 cut(s) 63, 208, 253, 392, 483
BsmFI GGGAC 3 cut(s) 66, 155, 604
BsnI GGCC 2 cut(s) 213, 1238
Bso31I GGTCTC 2 cut(s) 63, 483
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 766
Bsp143I GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
BspACI CCGC 2 cut(s) 1036, 1244
BspANI GGCC 2 cut(s) 213, 1238
BspCNI CTCAG 2 cut(s) 130, 636
BspDI ATCGAT 1 cut(s) 1116
BspEI TCCGGA 1 cut(s) 766
BspLI GGNNCC 1 cut(s) 1001
BspMI ACCTGC 2 cut(s) 332, 437
BspPI GGATC 4 cut(s) 12, 482, 953, 1000
BspQI GCTCTTC 1 cut(s) 1275
BspT107I GGYRCC 1 cut(s) 999
BspTNI GGTCTC 2 cut(s) 63, 483
BsrDI GCAATG 2 cut(s) 141, 927
BsrI ACTGG 1 cut(s) 1009
BssECI CCNNGG 3 cut(s) 192, 505, 828
BssMI GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
Bst2UI CCWGG 2 cut(s) 507, 663
Bst4CI ACNGT 4 cut(s) 57, 231, 829, 839
Bst6I CTCTTC 3 cut(s) 1166, 1202, 1275
BstAPI GCANNNNNTGC 1 cut(s) 415
BstBAI YACGTR 1 cut(s) 236
BstC8I GCNNGC 4 cut(s) 313, 1109, 1184, 1318
BstDEI CTNAG 3 cut(s) 117, 473, 623
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 5 cut(s) 262, 720, 853, 961, 1219
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 8 cut(s) 7, 47, 123, 490, 706, 961, 1008, 1116
BstMAI GTCTC 5 cut(s) 63, 208, 253, 392, 483
BstMBI GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
BstMCI CGRYCG 1 cut(s) 1239
BstMWI GCNNNNNNNGC 3 cut(s) 415, 688, 1265
BstNI CCWGG 2 cut(s) 507, 663
BstPAI GACNNNNGTC 1 cut(s) 384
BstSCI CCNGG 3 cut(s) 192, 505, 661
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 3 cut(s) 952, 979, 1020
BstV2I GAAGAC 2 cut(s) 39, 384
BstZI CGGCCG 1 cut(s) 1236
Bsu15I ATCGAT 1 cut(s) 1116
Bsu36I CCTNAGG 1 cut(s) 473
BsuRI GGCC 2 cut(s) 213, 1238
BsuTUI ATCGAT 1 cut(s) 1116
BtgI CCRYGG 1 cut(s) 828
BtsCI GGATG 5 cut(s) 262, 720, 853, 961, 1219
BtsI GCAGTG 2 cut(s) 715, 898
BtsIMutI CAGTG 3 cut(s) 715, 898, 903
BveI ACCTGC 2 cut(s) 332, 437
Cac8I GCNNGC 4 cut(s) 313, 1109, 1184, 1318
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 1116
Csp6I GTAC 6 cut(s) 542, 593, 612, 652, 833, 1212
CviAII CATG 2 cut(s) 535, 1058
CviQI GTAC 6 cut(s) 542, 593, 612, 652, 833, 1212
DdeI CTNAG 3 cut(s) 117, 473, 623
DpnI GATC 8 cut(s) 6, 46, 122, 489, 705, 960, 1007, 1115
DpnII GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
DraI TTTAAA 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 1236
EagI CGGCCG 1 cut(s) 1236
Eam1104I CTCTTC 3 cut(s) 1166, 1202, 1275
EarI CTCTTC 3 cut(s) 1166, 1202, 1275
EclXI CGGCCG 1 cut(s) 1236
Eco31I GGTCTC 2 cut(s) 63, 483
Eco52I CGGCCG 1 cut(s) 1236
Eco57I CTGAAG 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 935
EcoRII CCWGG 2 cut(s) 505, 661
EcoT22I ATGCAT 1 cut(s) 988
FaeI CATG 2 cut(s) 538, 1061
FaqI GGGAC 3 cut(s) 66, 155, 604
FatI CATG 2 cut(s) 534, 1057
Fnu4HI GCNGC 3 cut(s) 941, 968, 1034
FokI GGATG 5 cut(s) 269, 707, 860, 968, 1206
Fsp4HI GCNGC 3 cut(s) 941, 968, 1034
FspBI CTAG 2 cut(s) 305, 393
GlaI GCGC 1 cut(s) 269
GluI GCNGC 3 cut(s) 941, 968, 1034
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 2 cut(s) 213, 1238
HapII CCGG 3 cut(s) 194, 767, 962
HhaI GCGC 1 cut(s) 270
Hin1II CATG 2 cut(s) 538, 1061
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 4 cut(s) 168, 617, 1048, 1228
HpaII CCGG 3 cut(s) 194, 767, 962
HphI GGTGA 1 cut(s) 1172
Hpy166II GTNNAC 3 cut(s) 107, 835, 858
Hpy188I TCNGA 6 cut(s) 49, 97, 120, 167, 253, 1015
Hpy188III TCNNGA 5 cut(s) 83, 305, 677, 767, 919
Hpy8I GTNNAC 3 cut(s) 107, 835, 858
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 5 cut(s) 571, 665, 680, 1117, 1242
HpyCH4III ACNGT 4 cut(s) 57, 231, 829, 839
HpyCH4IV ACGT 2 cut(s) 235, 640
HpyCH4V TGCA 9 cut(s) 220, 281, 409, 720, 903, 932, 967, 986, 1057
HpyF10VI GCNNNNNNNGC 3 cut(s) 415, 688, 1265
HpyF3I CTNAG 3 cut(s) 117, 473, 623
HpySE526I ACGT 2 cut(s) 235, 640
Hsp92II CATG 2 cut(s) 538, 1061
HspAI GCGC 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 766
Kzo9I GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
LguI GCTCTTC 1 cut(s) 1275
LmnI GCTCC 1 cut(s) 1289
Lsp1109I GCAGC 3 cut(s) 952, 979, 1020
LweI GCATC 3 cut(s) 405, 700, 729
MaeI CTAG 2 cut(s) 305, 393
MaeII ACGT 2 cut(s) 235, 640
MaeIII GTNAC 4 cut(s) 57, 231, 368, 530
MalI GATC 8 cut(s) 6, 46, 122, 489, 705, 960, 1007, 1115
MboI GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 9 cut(s) 9, 401, 420, 521, 587, 708, 812, 1028, 1163
MlyI GAGTC 3 cut(s) 177, 626, 1222
MmeI TCCRAC 2 cut(s) 626, 1267
Mph1103I ATGCAT 1 cut(s) 988
MroI TCCGGA 1 cut(s) 766
MseI TTAA 3 cut(s) 21, 810, 992
MslI CAYNNNNRTG 2 cut(s) 326, 364
MspI CCGG 3 cut(s) 194, 767, 962
MspR9I CCNGG 3 cut(s) 194, 507, 663
MvaI CCWGG 2 cut(s) 507, 663
MwoI GCNNNNNNNGC 3 cut(s) 415, 688, 1265
NciI CCSGG 1 cut(s) 194
NdeII GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
NlaIII CATG 2 cut(s) 538, 1061
NlaIV GGNNCC 1 cut(s) 1001
NmuCI GTSAC 1 cut(s) 57
NsiI ATGCAT 1 cut(s) 988
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 935
PaqCI CACCTGC 2 cut(s) 332, 437
PciSI GCTCTTC 1 cut(s) 1275
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 1 cut(s) 1048
PkrI GCNGC 3 cut(s) 942, 969, 1035
PleI GAGTC 3 cut(s) 176, 625, 1222
PpsI GAGTC 3 cut(s) 176, 625, 1222
Ppu21I YACGTR 1 cut(s) 236
PshAI GACNNNNGTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 505, 661
PspGI CCWGG 2 cut(s) 505, 661
PspN4I GGNNCC 1 cut(s) 1001
PspPI GGNCC 1 cut(s) 212
PspXI VCTCGAGB 1 cut(s) 935
RsaI GTAC 6 cut(s) 543, 594, 613, 653, 834, 1213
RsaNI GTAC 6 cut(s) 542, 593, 612, 652, 833, 1212
RseI CAYNNNNRTG 2 cut(s) 326, 364
SapI GCTCTTC 1 cut(s) 1275
SaqAI TTAA 3 cut(s) 21, 810, 992
SatI GCNGC 3 cut(s) 941, 968, 1034
Sau3AI GATC 8 cut(s) 4, 44, 120, 487, 703, 958, 1005, 1113
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 3 cut(s) 177, 626, 1222
ScrFI CCNGG 3 cut(s) 194, 507, 663
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 3 cut(s) 405, 700, 729
Sfr274I CTCGAG 1 cut(s) 935
SlaI CTCGAG 1 cut(s) 935
SmiMI CAYNNNNRTG 2 cut(s) 326, 364
SmlI CTYRAG 3 cut(s) 452, 917, 935
SmoI CTYRAG 3 cut(s) 452, 917, 935
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 9 cut(s) 9, 401, 420, 521, 587, 708, 812, 1028, 1163
SsiI CCGC 2 cut(s) 1036, 1244
SspMI CTAG 2 cut(s) 305, 393
StyD4I CCNGG 3 cut(s) 192, 505, 661
TaaI ACNGT 4 cut(s) 57, 231, 829, 839
TaiI ACGT 2 cut(s) 238, 643
TaqI TCGA 8 cut(s) 292, 318, 546, 702, 712, 749, 936, 1116
TasI AATT 9 cut(s) 9, 401, 420, 521, 587, 708, 812, 1028, 1163
TatI WGTACW 1 cut(s) 1211
TfiI GAWTC 1 cut(s) 1048
Tru1I TTAA 3 cut(s) 21, 810, 992
Tru9I TTAA 3 cut(s) 21, 810, 992
TscAI CASTG 3 cut(s) 722, 905, 910
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 3 cut(s) 940, 967, 1033
Tsp45I GTSAC 1 cut(s) 57
TspDTI ATGAA 5 cut(s) 390, 414, 424, 450, 1184
TspRI CASTG 3 cut(s) 722, 905, 910
XapI RAATTY 3 cut(s) 401, 812, 1163
XbaI TCTAGA 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 935
XspI CTAG 2 cut(s) 305, 393
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.