RchiOBHm_Chr4g0424851

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016309
Physical Location & Seq
Reverse (-)
50439230 .. 50440522
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39409

Sequence Viewer

Length: 1293 bp
ATGGAAAGCAGTAGCAGATCAGAGGGACAGTCACAAACCAAAGACCACATCTCTCTAGAAATCTCTCCTGAAAATGGTGAACTTGTATCTCATATCAAAGAAAAGATGGAAGACATTGCTGTATCAGTGTCTATCTTCACTGTCCCTAACGAAAAAAAATGTGCCCCGGATTTTGTCTCCATTGGCCCTTTGCACTATAAACAAGTACGTGATAGCAAAGTCTCTGAAGATGATAAGTGGCGCTACTGCTATGCACTCCTCAGTCGAAAACCAAATCTAGGAGGAAGCCTTGACACCTGCGTGAAAGCCCTAAAACAAATGGAGCACAAGGCACGAAGATGCTACAGTGAAGACATCAAAGTCCCTAGTGATGAATTTGTGCAGTTGATGCTAATTGACAGCTGCTTCATCATTGAGCTATTTCTCAAGTATTCATACAAGAGCCTTAGGTCTCGCCGTGATCCCATCTTCAATAGGCCTGGTATGCTCATAGACTTGAGATGTAACATGGTGTTACTCGAAAACCAAATTCCCTTCTTTGTTATTCAGAGGTTATTTCAATTAGTACCACTTCCAACACAATGTACCGAGTCCCTCGGCGAACTTGCTACACGTTTCTTCAAGTACCTTATACCAGGAGAGTATCATCGCGAGCAAGAAGGTTATCATTTACTCGATCTAATTCGACACTGCATCCTCCCAACACATCCTCAGCTACAATCGACAGGCAAGAAAACTCCGGACTACTTGGATTGCGCAAAGAAGCTAAAACAAGCCGGTGTTAAGTTCCAGTGTGCCACCGCAGTACACAGTTTCTTGGACATCAAGTTTACTAATGGTGTGTTCAAAATGCCACCCCTTTTAGTCCATCACTGTACCGAAACACTCCTCAAGAACCTCATTGCACTCGAGGCGCGTCACATTGGGGATGATCCGGTGCAGCATGTCACATCTTACGCATACCTACTGCGGTGCCTAATCGTTTCCGAGAAAGATGTGAAATTGCTGCGGCGAAAACAGATTCTTGTGCATGAGGAGGATAAGGACGAGGAGGTTTTTAAAGTGTTGAAGAAGTTGTGCGATCAGATCGATGTGAAGGATTTTTACTATGTGAGGCTTTTCGATGATGTCGGTGAATTCGTGAAGAGAACGAAGAAGCAAAAGTTGAAGAGCAAATACCATCTCAACACTCCTTCGGCCGTTACGGTGCTTGTTGTTGCCATTTTGGCTCTTCTCCTCACATTTGTTGGAGCTTTTTTTTCTATACTCACATTCGCTCGCCACCATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

430

Amino Acids

49.61

Weight (kDa)

8.71

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 47 - 413 4.3e-95 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19590
malus_domestica MD16G1154900.v1.1
prunus_persica Prupe.1G118600_v2.0.a1
pyrus_communis pycom16g13130
rosa_chinensis RchiOBHm_Chr4g0424851
rosa_laevigata RLG00000007415
rosa_multiflora Rmu_sc0006754.1_g000008
rosa_roxburghii Rroxscaffold_5G00367070
rosa_rugosa Rorug04G0199600 Rorug04G0199700
rosa_samantha Rh4AG257400 Rh4BG262800 Rh4CG273700 Rh4DG258500
rosa_wichuraiana Rw4G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
AasI GACNNNNNNGTC 1 cut(s) 359
Acc16I TGCGCA 1 cut(s) 757
Acc36I ACCTGC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 972
AccII CGCG 2 cut(s) 651, 916
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 3 cut(s) 801, 970, 1009
AclWI GGATC 2 cut(s) 455, 926
AcoI YGGCCR 1 cut(s) 1197
AcsI RAATTY 3 cut(s) 374, 528, 1136
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 6 cut(s) 207, 567, 586, 626, 807, 877
AfiI CCNNNNNNNGG 2 cut(s) 74, 278
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 6 cut(s) 472, 560, 622, 847, 1069, 1168
AjnI CCWGG 2 cut(s) 478, 634
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 5 cut(s) 402, 418, 715, 766, 1253
AluI AGCT 5 cut(s) 402, 418, 715, 766, 1253
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 3 cut(s) 181, 226, 456
AlwI GGATC 2 cut(s) 455, 926
Ama87I CYCGRG 1 cut(s) 908
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 184, 476, 1197
ApeKI GCWGC 3 cut(s) 402, 940, 1006
ApoI RAATTY 3 cut(s) 374, 528, 1136
AspLEI GCGC 3 cut(s) 243, 758, 916
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 89, 1145
AvaI CYCGRG 1 cut(s) 908
AxyI CCTNAGG 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 972
BarI GAAGNNNNNNTAC 4 cut(s) 1088, 1120, 1160, 1192
BbsI GAAGAC 2 cut(s) 117, 357
Bbv12I GWGCWC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 3 cut(s) 389, 952, 993
BccI CCATC 4 cut(s) 100, 473, 876, 1188
BceAI ACGGC 2 cut(s) 441, 1184
BciT130I CCWGG 2 cut(s) 480, 636
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 3 cut(s) 181, 226, 456
BfaI CTAG 3 cut(s) 56, 278, 366
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 305
BglI GCCNNNNNGGC 1 cut(s) 1226
BisI GCNGC 4 cut(s) 403, 941, 1007, 1010
BlsI GCNGC 4 cut(s) 404, 942, 1008, 1011
Bme1390I CCNGG 3 cut(s) 167, 480, 636
BmeT110I CYCGRG 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 974
BmrFI CCNGG 3 cut(s) 167, 480, 636
BmsI GCATC 3 cut(s) 329, 378, 702
BpiI GAAGAC 2 cut(s) 117, 357
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 3 cut(s) 410, 517, 875
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 1 cut(s) 1089
BsaAI YACGTR 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 165, 595
BsaWI WCCGGW 2 cut(s) 739, 934
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 278
Bse118I RCCGGY 1 cut(s) 776
Bse1I ACTGG 1 cut(s) 790
Bse21I CCTNAGG 1 cut(s) 446
Bse3DI GCAATG 2 cut(s) 114, 900
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 2 cut(s) 480, 636
BseCI ATCGAT 1 cut(s) 1089
BseDI CCNNGG 2 cut(s) 165, 595
BseGI GGATG 3 cut(s) 693, 706, 934
BseLI CCNNNNNNNGG 2 cut(s) 74, 278
BseMI GCAATG 2 cut(s) 114, 900
BseMII CTCAG 2 cut(s) 274, 725
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 5 cut(s) 248, 878, 1049, 1064, 1226
BseSI GKGCMC 1 cut(s) 166
BseX3I CGGCCG 1 cut(s) 1197
BseXI GCAGC 3 cut(s) 389, 952, 993
BsgI GTGCAG 2 cut(s) 401, 959
Bsh1236I CGCG 2 cut(s) 651, 916
Bsh1285I CGRYCG 1 cut(s) 1200
BshFI GGCC 3 cut(s) 186, 478, 1199
BshNI GGYRCC 1 cut(s) 972
BshVI ATCGAT 1 cut(s) 1089
BsiEI CGRYCG 1 cut(s) 1200
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 908
BsiSI CCGG 4 cut(s) 167, 740, 777, 935
BslFI GGGAC 4 cut(s) 39, 128, 347, 577
BslI CCNNNNNNNGG 2 cut(s) 74, 278
BsmAI GTCTC 3 cut(s) 181, 226, 456
BsmFI GGGAC 4 cut(s) 39, 128, 347, 577
BsnI GGCC 3 cut(s) 186, 478, 1199
Bso31I GGTCTC 1 cut(s) 456
BsoBI CYCGRG 1 cut(s) 908
Bsp1286I GDGCHC 2 cut(s) 166, 327
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 3 cut(s) 801, 970, 1009
BspANI GGCC 3 cut(s) 186, 478, 1199
BspCNI CTCAG 2 cut(s) 273, 724
BspDI ATCGAT 1 cut(s) 1089
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 2 cut(s) 651, 916
BspLI GGNNCC 1 cut(s) 974
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 2 cut(s) 455, 926
BspQI GCTCTTC 2 cut(s) 1163, 1236
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 1 cut(s) 456
BsrDI GCAATG 2 cut(s) 114, 900
BsrFI RCCGGY 1 cut(s) 776
BsrI ACTGG 1 cut(s) 790
BssAI RCCGGY 1 cut(s) 776
BssECI CCNNGG 2 cut(s) 165, 595
BssMI GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
Bst2UI CCWGG 2 cut(s) 480, 636
Bst4CI ACNGT 6 cut(s) 30, 142, 347, 812, 875, 1207
Bst6I CTCTTC 3 cut(s) 1139, 1163, 1236
BstAPI GCANNNNNTGC 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 2 cut(s) 653, 1279
BstDEI CTNAG 3 cut(s) 260, 446, 711
BstF5I GGATG 3 cut(s) 693, 706, 934
BstFNI CGCG 2 cut(s) 651, 916
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 3 cut(s) 243, 758, 916
BstKTI GATC 6 cut(s) 20, 463, 679, 934, 1084, 1089
BstMAI GTCTC 3 cut(s) 181, 226, 456
BstMBI GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
BstMCI CGRYCG 1 cut(s) 1200
BstMWI GCNNNNNNNGC 4 cut(s) 388, 484, 911, 1226
BstNI CCWGG 2 cut(s) 480, 636
BstNSI RCATGY 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 165, 478, 634
BstSFI CTRYAG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 2 cut(s) 651, 916
BstV1I GCAGC 3 cut(s) 389, 952, 993
BstV2I GAAGAC 2 cut(s) 117, 357
BstZI CGGCCG 1 cut(s) 1197
Bsu15I ATCGAT 1 cut(s) 1089
Bsu36I CCTNAGG 1 cut(s) 446
BsuRI GGCC 3 cut(s) 186, 478, 1199
BsuTUI ATCGAT 1 cut(s) 1089
BtgZI GCGATG 1 cut(s) 632
BtsCI GGATG 3 cut(s) 693, 706, 934
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 6 cut(s) 132, 138, 352, 688, 797, 871
BtuMI TCGCGA 1 cut(s) 651
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 2 cut(s) 653, 1279
CfoI GCGC 3 cut(s) 243, 758, 916
Cfr10I RCCGGY 1 cut(s) 776
Cfr13I GGNCC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 1089
CseI GACGC 1 cut(s) 905
Csp6I GTAC 6 cut(s) 206, 566, 585, 625, 806, 876
CviAII CATG 4 cut(s) 508, 944, 1031, 1286
CviQI GTAC 6 cut(s) 206, 566, 585, 625, 806, 876
DdeI CTNAG 3 cut(s) 260, 446, 711
DpnI GATC 6 cut(s) 19, 462, 678, 933, 1083, 1088
DpnII GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
DraI TTTAAA 1 cut(s) 1060
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 1197
EagI CGGCCG 1 cut(s) 1197
Eam1104I CTCTTC 3 cut(s) 1139, 1163, 1236
EarI CTCTTC 3 cut(s) 1139, 1163, 1236
EclXI CGGCCG 1 cut(s) 1197
Eco147I AGGCCT 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 456
Eco52I CGGCCG 1 cut(s) 1197
Eco57I CTGAAG 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 908
EcoRI GAATTC 1 cut(s) 1136
EcoRII CCWGG 2 cut(s) 478, 634
FaeI CATG 4 cut(s) 511, 947, 1034, 1289
FaqI GGGAC 4 cut(s) 39, 128, 347, 577
FatI CATG 4 cut(s) 507, 943, 1030, 1285
Fnu4HI GCNGC 4 cut(s) 403, 941, 1007, 1010
FokI GGATG 3 cut(s) 680, 693, 941
Fsp4HI GCNGC 4 cut(s) 403, 941, 1007, 1010
FspBI CTAG 3 cut(s) 56, 278, 366
FspI TGCGCA 1 cut(s) 757
GlaI GCGC 3 cut(s) 242, 757, 915
GluI GCNGC 4 cut(s) 403, 941, 1007, 1010
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 3 cut(s) 186, 478, 1199
HapII CCGG 4 cut(s) 167, 740, 777, 935
HgaI GACGC 1 cut(s) 905
HhaI GCGC 3 cut(s) 243, 758, 916
Hin1II CATG 4 cut(s) 511, 947, 1034, 1289
Hin6I GCGC 3 cut(s) 241, 756, 914
HinP1I GCGC 3 cut(s) 241, 756, 914
HinfI GANTC 2 cut(s) 590, 1021
HpaII CCGG 4 cut(s) 167, 740, 777, 935
HphI GGTGA 2 cut(s) 89, 1145
Hpy166II GTNNAC 3 cut(s) 80, 808, 831
Hpy188I TCNGA 5 cut(s) 22, 226, 549, 988, 1086
Hpy188III TCNNGA 6 cut(s) 56, 68, 650, 740, 892, 1141
Hpy8I GTNNAC 3 cut(s) 80, 808, 831
HpyAV CCTTC 4 cut(s) 544, 653, 1090, 1203
HpyCH4III ACNGT 6 cut(s) 30, 142, 347, 812, 875, 1207
HpyCH4IV ACGT 2 cut(s) 208, 613
HpyCH4V TGCA 7 cut(s) 193, 254, 382, 693, 905, 940, 1030
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 484, 911, 1226
HpyF3I CTNAG 3 cut(s) 260, 446, 711
HpySE526I ACGT 2 cut(s) 208, 613
Hsp92II CATG 4 cut(s) 511, 947, 1034, 1289
HspAI GCGC 3 cut(s) 241, 756, 914
Kpn2I TCCGGA 1 cut(s) 739
Kzo9I GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
LguI GCTCTTC 2 cut(s) 1163, 1236
LmnI GCTCC 2 cut(s) 322, 1250
Lsp1109I GCAGC 3 cut(s) 389, 952, 993
LweI GCATC 3 cut(s) 329, 378, 702
MaeI CTAG 3 cut(s) 56, 278, 366
MaeII ACGT 2 cut(s) 208, 613
MaeIII GTNAC 6 cut(s) 30, 503, 513, 917, 946, 1201
MalI GATC 6 cut(s) 19, 462, 678, 933, 1083, 1088
MboI GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
MhlI GDGCHC 2 cut(s) 166, 327
MluCI AATT 7 cut(s) 374, 393, 528, 560, 681, 1001, 1136
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 2 cut(s) 599, 1228
MroI TCCGGA 1 cut(s) 739
MseI TTAA 2 cut(s) 783, 1059
MslI CAYNNNNRTG 2 cut(s) 299, 337
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 4 cut(s) 167, 740, 777, 935
MspR9I CCNGG 3 cut(s) 167, 480, 636
MvaI CCWGG 2 cut(s) 480, 636
MvnI CGCG 2 cut(s) 651, 916
MwoI GCNNNNNNNGC 4 cut(s) 388, 484, 911, 1226
NciI CCSGG 1 cut(s) 167
NdeII GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
NlaIII CATG 4 cut(s) 511, 947, 1034, 1289
NlaIV GGNNCC 1 cut(s) 974
NmeAIII GCCGAG 1 cut(s) 576
NmuCI GTSAC 3 cut(s) 30, 917, 946
NruI TCGCGA 1 cut(s) 651
NsbI TGCGCA 1 cut(s) 757
NspI RCATGY 1 cut(s) 947
OliI CACNNNNGTG 1 cut(s) 299
PaeR7I CTCGAG 1 cut(s) 908
PaqCI CACCTGC 1 cut(s) 305
PceI AGGCCT 1 cut(s) 478
PciSI GCTCTTC 2 cut(s) 1163, 1236
PcsI WCGNNNNNNNCGW 1 cut(s) 1137
PfeI GAWTC 1 cut(s) 1021
PkrI GCNGC 4 cut(s) 404, 942, 1008, 1011
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
Ppu21I YACGTR 1 cut(s) 209
Psp6I CCWGG 2 cut(s) 478, 634
PspGI CCWGG 2 cut(s) 478, 634
PspN4I GGNNCC 1 cut(s) 974
PspPI GGNCC 1 cut(s) 185
PspXI VCTCGAGB 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 402
RruI TCGCGA 1 cut(s) 651
RsaI GTAC 6 cut(s) 207, 567, 586, 626, 807, 877
RsaNI GTAC 6 cut(s) 206, 566, 585, 625, 806, 876
RseI CAYNNNNRTG 2 cut(s) 299, 337
SapI GCTCTTC 2 cut(s) 1163, 1236
SaqAI TTAA 2 cut(s) 783, 1059
SatI GCNGC 4 cut(s) 403, 941, 1007, 1010
Sau3AI GATC 6 cut(s) 17, 460, 676, 931, 1081, 1086
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 167, 480, 636
SduI GDGCHC 2 cut(s) 166, 327
SfaNI GCATC 3 cut(s) 329, 378, 702
SfcI CTRYAG 1 cut(s) 343
Sfr274I CTCGAG 1 cut(s) 908
SlaI CTCGAG 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 299, 337
SmlI CTYRAG 4 cut(s) 425, 496, 890, 908
SmoI CTYRAG 4 cut(s) 425, 496, 890, 908
Sse9I AATT 7 cut(s) 374, 393, 528, 560, 681, 1001, 1136
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 3 cut(s) 801, 970, 1009
SspMI CTAG 3 cut(s) 56, 278, 366
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 3 cut(s) 165, 478, 634
TaaI ACNGT 6 cut(s) 30, 142, 347, 812, 875, 1207
TaiI ACGT 2 cut(s) 211, 616
TaqI TCGA 8 cut(s) 265, 519, 675, 685, 722, 909, 1089, 1122
TasI AATT 7 cut(s) 374, 393, 528, 560, 681, 1001, 1136
TatI WGTACW 1 cut(s) 805
TauI GCSGC 1 cut(s) 1012
TfiI GAWTC 1 cut(s) 1021
Tru1I TTAA 2 cut(s) 783, 1059
Tru9I TTAA 2 cut(s) 783, 1059
TscAI CASTG 6 cut(s) 132, 145, 352, 695, 797, 878
TseFI GTSAC 3 cut(s) 30, 917, 946
TseI GCWGC 3 cut(s) 402, 940, 1006
Tsp45I GTSAC 3 cut(s) 30, 917, 946
TspDTI ATGAA 3 cut(s) 387, 397, 423
TspRI CASTG 6 cut(s) 132, 145, 352, 695, 797, 878
XapI RAATTY 3 cut(s) 374, 528, 1136
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 947
XhoI CTCGAG 1 cut(s) 908
XspI CTAG 3 cut(s) 56, 278, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.