Rh4AG257400

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016309
Physical Location & Seq
Reverse (-)
57201834 .. 57204242
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG257400.1

Sequence Viewer

Length: 1332 bp
ATGAATCAAATTGAGATTTTAAAAAACATGGAAAGCAGTAGCAGATCAGAGGGACAGTCACAAACCAAAGACCACATCTCTCTTGAAATCTCTCCTGAAAATGGTGAACTTGTATCTCATATCAAAGAAAAGATGGAAGACGTTGCTGTATCAGTCTCTATCTTCAGAGTCCCTAGCGAAAAAATATGTGCCCCGGATTTTGTCTCCATTGGCCCTTTGCACTATAAACAAGTACGTGATAGCAAAGTCTCTGAAGACGATAAGTGGCGCTACTGCTATGCACTCCTCAGTCGAAAACCAAATCTAGGAGGAAGCCTTGACACCTGCGTGAAAGCCCTAAAACAAATGGAGCACAAGGCACGAAGATGCTACAGTGAAGACATCAAAGTCCCTAGTGATGAATTTGTGCAGTTGATGCTAATTGACAGCTGCTTCATCATTGAGCTATTTCTCAAGTATTCATACAAGAGCCTTAGGTCTCGCCGTGATCCCATCTTCAATAGGCCTGGTATGCTCATAGACTTGAGATGTAACATGGTGTTGCTCGAAAACCAAATTCCCTTCTTTGTTATTCAGAGGTTATTTCAATTAGTACCACTTCCAACACAATGTACCGAGTCCCTCAGCGAACTTGCTGCACGTTTCTTCAAGTACCTTATACCAGGAGAGTATCATCGCGAGCAAGAAGGTTATCATTTACTCGATCTAATTCGACACTGCATCCTCCCAACATATCCTAAGCTACAATCGACAGGCAAGAAAACTCCAGACTACTTGGATTGTGCAAAGAAGCTAAAACAAGCAGGGGTTAAGTTCCAGTGTGCTACCGCGGTACACAGTTTCTTGGACATCAAGTTTACTAATGGTGTGTTCAAAATGCCACCCCTTTTAATCCATCACTGTACCGAAACACTCCTCAAGAACCTCATTGCACTCGAGGCGCGTCACATTGGGGATGATCCTGTGCAGCATGTCACATCTTACGCATACCTAATGGGGTGCCTAATTGGTTCCGAGAAAGATGTGAAATGGCTGCGGCGAAAACAGATTCTTGTGCATGAGGAGGAGAAGGACAAGGAGGTTTTTGAAGTGTTGAAGAAGTTGTGCGATCAGATCGATGTGAAGGATTTTTACTATGTGAAGCTTTTCGATGAGGTGGGTGAATTCGTGAACAGAAAAAGCTGGCACACAAAGAAGCAAAAGTTGAAGAGCAAATACCATCTCAACACTCCTTCGGCGGTTACGGTGCTTGTTGTTGCCGTTTTGGCTCTTCTTCTCACATTTGTTGGAGCTTTTTTTTCTATACTCACATTCGCTCGCCACCATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

443

Amino Acids

51.19

Weight (kDa)

8.7

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 57 - 426 2.7e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19590
malus_domestica MD16G1154900.v1.1
prunus_persica Prupe.1G118600_v2.0.a1
pyrus_communis pycom16g13130
rosa_chinensis RchiOBHm_Chr4g0424851
rosa_laevigata RLG00000007415
rosa_multiflora Rmu_sc0006754.1_g000008
rosa_roxburghii Rroxscaffold_5G00367070
rosa_rugosa Rorug04G0199600 Rorug04G0199700
rosa_samantha Rh4AG257400 Rh4BG262800 Rh4CG273700 Rh4DG258500
rosa_wichuraiana Rw4G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 332
AasI GACNNNNNNGTC 1 cut(s) 386
Acc36I ACCTGC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 999
AccII CGCG 3 cut(s) 678, 830, 943
AciI CCGC 4 cut(s) 828, 830, 1036, 1238
AclWI GGATC 2 cut(s) 482, 953
AcsI RAATTY 3 cut(s) 401, 555, 1163
AcuI CTGAAG 2 cut(s) 148, 273
AfaI GTAC 6 cut(s) 234, 594, 613, 653, 834, 904
AfiI CCNNNNNNNGG 2 cut(s) 101, 305
AgsI TTSAA 8 cut(s) 86, 499, 587, 649, 874, 1088, 1096, 1207
AjnI CCWGG 2 cut(s) 505, 661
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 7 cut(s) 429, 445, 742, 793, 1144, 1182, 1292
AluI AGCT 7 cut(s) 429, 445, 742, 793, 1144, 1182, 1292
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 4 cut(s) 160, 208, 253, 483
AlwI GGATC 2 cut(s) 482, 953
Ama87I CYCGRG 1 cut(s) 935
AoxI GGCC 2 cut(s) 211, 503
ApeKI GCWGC 4 cut(s) 429, 635, 967, 1033
ApoI RAATTY 3 cut(s) 401, 555, 1163
Asp700I GAANNNNTTC 1 cut(s) 1145
AspLEI GCGC 2 cut(s) 270, 943
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 116, 1172
AvaI CYCGRG 1 cut(s) 935
AxyI CCTNAGG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 999
BarI GAAGNNNNNNTAC 4 cut(s) 1115, 1147, 1199, 1231
BbsI GAAGAC 3 cut(s) 144, 261, 384
Bbv12I GWGCWC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 623
BbvI GCAGC 4 cut(s) 416, 622, 979, 1020
BccI CCATC 4 cut(s) 127, 500, 903, 1227
BceAI ACGGC 2 cut(s) 468, 1244
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciT130I CCWGG 2 cut(s) 507, 663
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 4 cut(s) 160, 208, 253, 483
BfaI CTAG 3 cut(s) 174, 305, 393
BfmI CTRYAG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 1265
BisI GCNGC 5 cut(s) 430, 636, 968, 1034, 1037
BlsI GCNGC 5 cut(s) 431, 637, 969, 1035, 1038
Bme1390I CCNGG 3 cut(s) 194, 507, 663
BmeT110I CYCGRG 1 cut(s) 935
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 1001, 1012
BmrFI CCNGG 3 cut(s) 194, 507, 663
BmsI GCATC 3 cut(s) 356, 405, 729
BpiI GAAGAC 3 cut(s) 144, 261, 384
BpmI CTGGAG 1 cut(s) 750
Bpu10I CCTNAGC 2 cut(s) 623, 738
BpuEI CTTGAG 3 cut(s) 437, 544, 902
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 1116
BsaAI YACGTR 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 192, 828
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 305
Bse1I ACTGG 1 cut(s) 817
Bse21I CCTNAGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 2 cut(s) 507, 663
BseCI ATCGAT 1 cut(s) 1116
BseDI CCNNGG 2 cut(s) 192, 828
BseGI GGATG 2 cut(s) 720, 961
BseLI CCNNNNNNNGG 2 cut(s) 101, 305
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 2 cut(s) 301, 637
BseNI ACTGG 1 cut(s) 817
BseRI GAGGAG 4 cut(s) 275, 905, 1076, 1079
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 4 cut(s) 416, 622, 979, 1020
BsgI GTGCAG 3 cut(s) 428, 621, 986
Bsh1236I CGCG 3 cut(s) 678, 830, 943
BshFI GGCC 2 cut(s) 213, 505
BshNI GGYRCC 1 cut(s) 999
BshVI ATCGAT 1 cut(s) 1116
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 4 cut(s) 66, 155, 374, 604
BslI CCNNNNNNNGG 2 cut(s) 101, 305
BsmAI GTCTC 4 cut(s) 160, 208, 253, 483
BsmFI GGGAC 4 cut(s) 66, 155, 374, 604
BsnI GGCC 2 cut(s) 213, 505
Bso31I GGTCTC 1 cut(s) 483
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 2 cut(s) 193, 354
Bsp143I GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
Bsp68I TCGCGA 1 cut(s) 678
BspACI CCGC 4 cut(s) 828, 830, 1036, 1238
BspANI GGCC 2 cut(s) 213, 505
BspCNI CTCAG 2 cut(s) 300, 636
BspDI ATCGAT 1 cut(s) 1116
BspFNI CGCG 3 cut(s) 678, 830, 943
BspLI GGNNCC 2 cut(s) 1001, 1012
BspMI ACCTGC 1 cut(s) 332
BspPI GGATC 2 cut(s) 482, 953
BspQI GCTCTTC 2 cut(s) 1202, 1275
BspT107I GGYRCC 1 cut(s) 999
BspTNI GGTCTC 1 cut(s) 483
BsrDI GCAATG 1 cut(s) 927
BsrI ACTGG 1 cut(s) 817
BssECI CCNNGG 2 cut(s) 192, 828
BssMI GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
Bst2UI CCWGG 2 cut(s) 507, 663
Bst4CI ACNGT 5 cut(s) 57, 374, 839, 902, 1246
Bst6I CTCTTC 2 cut(s) 1202, 1275
BstAPI GCANNNNNTGC 1 cut(s) 415
BstBAI YACGTR 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 680, 1184, 1318
BstDEI CTNAG 4 cut(s) 287, 473, 623, 738
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 2 cut(s) 720, 961
BstFNI CGCG 3 cut(s) 678, 830, 943
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 2 cut(s) 270, 943
BstKTI GATC 6 cut(s) 47, 490, 706, 961, 1111, 1116
BstMAI GTCTC 4 cut(s) 160, 208, 253, 483
BstMBI GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
BstMWI GCNNNNNNNGC 4 cut(s) 415, 511, 938, 1265
BstNI CCWGG 2 cut(s) 507, 663
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 3 cut(s) 192, 505, 661
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 3 cut(s) 678, 830, 943
BstV1I GCAGC 4 cut(s) 416, 622, 979, 1020
BstV2I GAAGAC 3 cut(s) 144, 261, 384
Bsu15I ATCGAT 1 cut(s) 1116
Bsu36I CCTNAGG 1 cut(s) 473
BsuRI GGCC 2 cut(s) 213, 505
BsuTUI ATCGAT 1 cut(s) 1116
BtgI CCRYGG 1 cut(s) 828
BtgZI GCGATG 1 cut(s) 659
BtsCI GGATG 2 cut(s) 720, 961
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 4 cut(s) 379, 715, 824, 898
BtuMI TCGCGA 1 cut(s) 678
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 3 cut(s) 680, 1184, 1318
CfoI GCGC 2 cut(s) 270, 943
Cfr13I GGNCC 1 cut(s) 212
Cfr42I CCGCGG 1 cut(s) 831
ClaI ATCGAT 1 cut(s) 1116
CseI GACGC 1 cut(s) 932
Csp6I GTAC 6 cut(s) 233, 593, 612, 652, 833, 903
CviAII CATG 5 cut(s) 28, 535, 971, 1058, 1325
CviQI GTAC 6 cut(s) 233, 593, 612, 652, 833, 903
DdeI CTNAG 4 cut(s) 287, 473, 623, 738
DpnI GATC 6 cut(s) 46, 489, 705, 960, 1110, 1115
DpnII GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
DraI TTTAAA 1 cut(s) 21
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 2 cut(s) 1202, 1275
EarI CTCTTC 2 cut(s) 1202, 1275
Eco147I AGGCCT 1 cut(s) 505
Eco31I GGTCTC 1 cut(s) 483
Eco57I CTGAAG 2 cut(s) 148, 273
Eco81I CCTNAGG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 1163
EcoRII CCWGG 2 cut(s) 505, 661
FaeI CATG 5 cut(s) 31, 538, 974, 1061, 1328
FaqI GGGAC 4 cut(s) 66, 155, 374, 604
FatI CATG 5 cut(s) 27, 534, 970, 1057, 1324
Fnu4HI GCNGC 5 cut(s) 430, 636, 968, 1034, 1037
FokI GGATG 2 cut(s) 707, 968
Fsp4HI GCNGC 5 cut(s) 430, 636, 968, 1034, 1037
FspBI CTAG 3 cut(s) 174, 305, 393
GlaI GCGC 2 cut(s) 269, 942
GluI GCNGC 5 cut(s) 430, 636, 968, 1034, 1037
GsuI CTGGAG 1 cut(s) 750
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 2 cut(s) 213, 505
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 932
HhaI GCGC 2 cut(s) 270, 943
Hin1II CATG 5 cut(s) 31, 538, 974, 1061, 1328
Hin6I GCGC 2 cut(s) 268, 941
HinP1I GCGC 2 cut(s) 268, 941
HindIII AAGCTT 1 cut(s) 1142
HinfI GANTC 4 cut(s) 4, 168, 617, 1048
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 116, 1172
Hpy166II GTNNAC 4 cut(s) 107, 835, 858, 1171
Hpy188I TCNGA 6 cut(s) 49, 167, 253, 576, 1015, 1113
Hpy188III TCNNGA 6 cut(s) 83, 95, 677, 767, 919, 1168
Hpy8I GTNNAC 4 cut(s) 107, 835, 858, 1171
HpyAV CCTTC 5 cut(s) 571, 680, 1063, 1117, 1242
HpyCH4III ACNGT 5 cut(s) 57, 374, 839, 902, 1246
HpyCH4IV ACGT 3 cut(s) 141, 235, 640
HpyCH4V TGCA 9 cut(s) 220, 281, 409, 638, 720, 785, 932, 967, 1057
HpyF10VI GCNNNNNNNGC 4 cut(s) 415, 511, 938, 1265
HpyF3I CTNAG 4 cut(s) 287, 473, 623, 738
HpySE526I ACGT 3 cut(s) 141, 235, 640
Hsp92II CATG 5 cut(s) 31, 538, 974, 1061, 1328
HspAI GCGC 2 cut(s) 268, 941
KspI CCGCGG 1 cut(s) 831
Kzo9I GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
LguI GCTCTTC 2 cut(s) 1202, 1275
LmnI GCTCC 2 cut(s) 349, 1289
Lsp1109I GCAGC 4 cut(s) 416, 622, 979, 1020
LweI GCATC 3 cut(s) 356, 405, 729
MaeI CTAG 3 cut(s) 174, 305, 393
MaeII ACGT 3 cut(s) 141, 235, 640
MaeIII GTNAC 5 cut(s) 57, 530, 944, 973, 1240
MalI GATC 6 cut(s) 46, 489, 705, 960, 1110, 1115
MboI GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
MhlI GDGCHC 2 cut(s) 193, 354
MluCI AATT 8 cut(s) 9, 401, 420, 555, 587, 708, 1005, 1163
MlyI GAGTC 2 cut(s) 177, 626
MmeI TCCRAC 2 cut(s) 626, 1267
MroXI GAANNNNTTC 1 cut(s) 1145
MseI TTAA 3 cut(s) 20, 810, 890
MslI CAYNNNNRTG 2 cut(s) 326, 364
MspA1I CMGCKG 2 cut(s) 429, 830
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 194, 507, 663
MvaI CCWGG 2 cut(s) 507, 663
MvnI CGCG 3 cut(s) 678, 830, 943
MwoI GCNNNNNNNGC 4 cut(s) 415, 511, 938, 1265
NciI CCSGG 1 cut(s) 194
NdeII GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
NlaIII CATG 5 cut(s) 31, 538, 974, 1061, 1328
NlaIV GGNNCC 2 cut(s) 1001, 1012
NmuCI GTSAC 3 cut(s) 57, 944, 973
NruI TCGCGA 1 cut(s) 678
NspI RCATGY 1 cut(s) 974
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 935
PaqCI CACCTGC 1 cut(s) 332
PceI AGGCCT 1 cut(s) 505
PciSI GCTCTTC 2 cut(s) 1202, 1275
PdmI GAANNNNTTC 1 cut(s) 1145
PfeI GAWTC 2 cut(s) 4, 1048
PkrI GCNGC 5 cut(s) 431, 637, 969, 1035, 1038
PleI GAGTC 2 cut(s) 176, 625
PpsI GAGTC 2 cut(s) 176, 625
Ppu21I YACGTR 1 cut(s) 236
Psp6I CCWGG 2 cut(s) 505, 661
PspGI CCWGG 2 cut(s) 505, 661
PspN4I GGNNCC 2 cut(s) 1001, 1012
PspPI GGNCC 1 cut(s) 212
PspXI VCTCGAGB 1 cut(s) 935
PvuII CAGCTG 1 cut(s) 429
RruI TCGCGA 1 cut(s) 678
RsaI GTAC 6 cut(s) 234, 594, 613, 653, 834, 904
RsaNI GTAC 6 cut(s) 233, 593, 612, 652, 833, 903
RseI CAYNNNNRTG 2 cut(s) 326, 364
SacII CCGCGG 1 cut(s) 831
SapI GCTCTTC 2 cut(s) 1202, 1275
SaqAI TTAA 3 cut(s) 20, 810, 890
SatI GCNGC 5 cut(s) 430, 636, 968, 1034, 1037
Sau3AI GATC 6 cut(s) 44, 487, 703, 958, 1108, 1113
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 2 cut(s) 177, 626
ScrFI CCNGG 3 cut(s) 194, 507, 663
SduI GDGCHC 2 cut(s) 193, 354
SfaNI GCATC 3 cut(s) 356, 405, 729
SfcI CTRYAG 1 cut(s) 370
Sfr274I CTCGAG 1 cut(s) 935
Sfr303I CCGCGG 1 cut(s) 831
SgrBI CCGCGG 1 cut(s) 831
SlaI CTCGAG 1 cut(s) 935
SmiMI CAYNNNNRTG 2 cut(s) 326, 364
SmlI CTYRAG 4 cut(s) 452, 523, 917, 935
SmoI CTYRAG 4 cut(s) 452, 523, 917, 935
Sse9I AATT 8 cut(s) 9, 401, 420, 555, 587, 708, 1005, 1163
SseBI AGGCCT 1 cut(s) 505
SsiI CCGC 4 cut(s) 828, 830, 1036, 1238
SspMI CTAG 3 cut(s) 174, 305, 393
StuI AGGCCT 1 cut(s) 505
StyD4I CCNGG 3 cut(s) 192, 505, 661
TaaI ACNGT 5 cut(s) 57, 374, 839, 902, 1246
TaiI ACGT 3 cut(s) 144, 238, 643
TaqI TCGA 8 cut(s) 292, 546, 702, 712, 749, 936, 1116, 1149
TasI AATT 8 cut(s) 9, 401, 420, 555, 587, 708, 1005, 1163
TauI GCSGC 1 cut(s) 1039
TfiI GAWTC 2 cut(s) 4, 1048
Tru1I TTAA 3 cut(s) 20, 810, 890
Tru9I TTAA 3 cut(s) 20, 810, 890
TscAI CASTG 4 cut(s) 379, 722, 824, 905
TseFI GTSAC 3 cut(s) 57, 944, 973
TseI GCWGC 4 cut(s) 429, 635, 967, 1033
Tsp45I GTSAC 3 cut(s) 57, 944, 973
TspDTI ATGAA 4 cut(s) 17, 414, 424, 450
TspRI CASTG 4 cut(s) 379, 722, 824, 905
XapI RAATTY 3 cut(s) 401, 555, 1163
XceI RCATGY 1 cut(s) 974
XhoI CTCGAG 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 1145
XspI CTAG 3 cut(s) 174, 305, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.